LOCUS       NM_152396               2618 bp    mRNA    linear   PRI 08-DEC-2024
DEFINITION  Homo sapiens methyltransferase 6, tRNA N3-cytidine (METTL6),
            transcript variant 1, mRNA.
ACCESSION   NM_152396
VERSION     NM_152396.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Li,S., Zhou,H., Liao,S., Wang,X., Zhu,Z., Zhang,J. and Xu,C.
  TITLE     Structural basis for METTL6-mediated m3C RNA methylation
  JOURNAL   Biochem Biophys Res Commun 589, 159-164 (2022)
   PUBMED   34922197
  REMARK    GeneRIF: Structural basis for METTL6-mediated m3C RNA methylation.
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Chen,R., Zhou,J., Liu,L., Mao,X.L., Zhou,X. and Xie,W.
  TITLE     Crystal structure of human METTL6, the m3C methyltransferase
  JOURNAL   Commun Biol 4 (1), 1361 (2021)
   PUBMED   34862464
  REMARK    GeneRIF: Crystal structure of human METTL6, the m(3)C
            methyltransferase.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2618)
  AUTHORS   Mao,X.L., Li,Z.H., Huang,M.H., Wang,J.T., Zhou,J.B., Li,Q.R.,
            Xu,H., Wang,X.J. and Zhou,X.L.
  TITLE     Mutually exclusive substrate selection strategy by human m3C RNA
            transferases METTL2A and METTL6
  JOURNAL   Nucleic Acids Res 49 (14), 8309-8323 (2021)
   PUBMED   34268557
  REMARK    GeneRIF: Mutually exclusive substrate selection strategy by human
            m3C RNA transferases METTL2A and METTL6.
REFERENCE   4  (bases 1 to 2618)
  AUTHORS   Ignatova,V.V., Kaiser,S., Ho,J.S.Y., Bing,X., Stolz,P., Tan,Y.X.,
            Lee,C.L., Gay,F.P.H., Lastres,P.R., Gerlini,R., Rathkolb,B.,
            Aguilar-Pimentel,A., Sanz-Moreno,A., Klein-Rodewald,T.,
            Calzada-Wack,J., Ibragimov,E., Valenta,M., Lukauskas,S., Pavesi,A.,
            Marschall,S., Leuchtenberger,S., Fuchs,H., Gailus-Durner,V., de
            Angelis,M.H., Bultmann,S., Rando,O.J., Guccione,E., Kellner,S.M.
            and Schneider,R.
  TITLE     METTL6 is a tRNA m3C methyltransferase that regulates pluripotency
            and tumor cell growth
  JOURNAL   Sci Adv 6 (35), eaaz4551 (2020)
   PUBMED   32923617
  REMARK    GeneRIF: METTL6 is a tRNA m(3)C methyltransferase that regulates
            pluripotency and tumor cell growth.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2618)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 2618)
  AUTHORS   Xu,L., Liu,X., Sheng,N., Oo,K.S., Liang,J., Chionh,Y.H., Xu,J.,
            Ye,F., Gao,Y.G., Dedon,P.C. and Fu,X.Y.
  TITLE     Three distinct 3-methylcytidine (m3C) methyltransferases modify
            tRNA and mRNA in mice and humans
  JOURNAL   J Biol Chem 292 (35), 14695-14703 (2017)
   PUBMED   28655767
  REMARK    GeneRIF: Data, including data from studies using mutant/knockout
            mice and cell lines from such mice, suggest that METTL2 and METTL6
            (but not METTL8) contribute to post-transcriptional methylation of
            cytidine (formation of 3-methylcytidine) in tRNA; METTL2 methylates
            tRNA(Thr) and tRNA(Arg); METTL6 interacts with seryl-tRNA
            synthetase and methylates tRNA(Ser); METTL8 catalyzes
            post-transcriptional methylation of cytidine in mRNA.
REFERENCE   7  (bases 1 to 2618)
  AUTHORS   Fleming,I.M., Paris,Z., Gaston,K.W., Balakrishnan,R., Fredrick,K.,
            Rubio,M.A. and Alfonzo,J.D.
  TITLE     A tRNA methyltransferase paralog is important for ribosome
            stability and cell division in Trypanosoma brucei
  JOURNAL   Sci Rep 6, 21438 (2016)
   PUBMED   26888608
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 2618)
  AUTHORS   McArdle,P.F., Parsa,A., Chang,Y.P., Weir,M.R., O'Connell,J.R.,
            Mitchell,B.D. and Shuldiner,A.R.
  TITLE     Association of a common nonsynonymous variant in GLUT9 with serum
            uric acid levels in old order amish
  JOURNAL   Arthritis Rheum 58 (9), 2874-2881 (2008)
   PUBMED   18759275
REFERENCE   9  (bases 1 to 2618)
  AUTHORS   Auffray,C., Behar,G., Bois,F., Bouchier,C., Da Silva,C.,
            Devignes,M.D., Duprat,S., Houlgatte,R., Jumeau,M.N., Lamy,B. et al.
  TITLE     [IMAGE: molecular integration of the analysis of the human genome
            and its expression]
  JOURNAL   C R Acad Sci III 318 (2), 263-272 (1995)
   PUBMED   7757816
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BG701912.1, AC027125.5, BC022400.1, CX872865.1 and Z44406.1.
            
            On Feb 27, 2020 this sequence version replaced NM_152396.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853563.22212.1, BC022400.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000383790.8/ ENSP00000373300.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4                 BG701912.1         6-9
            5-5                 AC027125.5         65492-65492
            6-75                BG701912.1         11-80
            76-1033             BC022400.1         36-993
            1034-1330           CX872865.1         406-702
            1331-2506           AC027125.5         81985-83160
            2507-2618           Z44406.1           103-214
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p25.1"
     gene            1..2618
                     /gene="METTL6"
                     /gene_synonym="hMETTL6"
                     /note="methyltransferase 6, tRNA N3-cytidine"
                     /db_xref="GeneID:131965"
                     /db_xref="HGNC:HGNC:28343"
                     /db_xref="MIM:618903"
     exon            1..143
                     /gene="METTL6"
                     /gene_synonym="hMETTL6"
                     /inference="alignment:Splign:2.1.0"
     exon            144..492
                     /gene="METTL6"
                     /gene_synonym="hMETTL6"
                     /inference="alignment:Splign:2.1.0"
     CDS             268..1122
                     /gene="METTL6"
                     /gene_synonym="hMETTL6"
                     /EC_number="2.1.1.-"
                     /note="isoform 1 is encoded by transcript variant 1;
                     methyltransferase-like protein 6; tRNA N(3)-methylcytidine
                     methyltransferase METTL6; methyltransferase like 6;
                     methyltransferase 6, methylcytidine; methyltransferase 6,
                     RNA N3-cytidine; tRNA N(3)-cytidine methyltransferase
                     METTL6"
                     /codon_start=1
                     /product="tRNA N(3)-cytidine methyltransferase METTL6
                     isoform 1"
                     /protein_id="NP_689609.2"
                     /db_xref="CCDS:CCDS43056.1"
                     /db_xref="GeneID:131965"
                     /db_xref="HGNC:HGNC:28343"
                     /db_xref="MIM:618903"
                     /translation="MASLQRKGLQARILTSEEEEKLKRDQTLVSDFKQQKLEQEAQKN
                     WDLFYKRNSTNFFKDRHWTTREFEELRSCREFEDQKLTMLEAGCGVGNCLFPLLEEDP
                     NIFAYACDFSPRAIEYVKQNPLYDTERCKVFQCDLTKDDLLDHVPPESVDVVMLIFVL
                     SAVHPDKMHLVLQNIYKVLKPGKSVLFRDYGLYDHAMLRFKASSKLGENFYVRQDGTR
                     SYFFTDDFLAQLFMDTGYEEVVNEYVFRETVNKKEGLCVPRVFLQSKFLKPPKNPSPV
                     VLGLDPKS"
     exon            493..627
                     /gene="METTL6"
                     /gene_synonym="hMETTL6"
                     /inference="alignment:Splign:2.1.0"
     exon            628..798
                     /gene="METTL6"
                     /gene_synonym="hMETTL6"
                     /inference="alignment:Splign:2.1.0"
     exon            799..940
                     /gene="METTL6"
                     /gene_synonym="hMETTL6"
                     /inference="alignment:Splign:2.1.0"
     exon            941..2618
                     /gene="METTL6"
                     /gene_synonym="hMETTL6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2252..2257
                     /regulatory_class="polyA_signal_sequence"
                     /gene="METTL6"
                     /gene_synonym="hMETTL6"
                     /note="hexamer: AATAAA"
     polyA_site      2274
                     /gene="METTL6"
                     /gene_synonym="hMETTL6"
                     /note="major polyA site"
     regulatory      2457..2462
                     /regulatory_class="polyA_signal_sequence"
                     /gene="METTL6"
                     /gene_synonym="hMETTL6"
                     /note="hexamer: AATAAG"
     polyA_site      2488
                     /gene="METTL6"
                     /gene_synonym="hMETTL6"
ORIGIN      
        1 gttcctagcg gaagtggcgg gggcggtgcc atggcaaaag aactcccgga taatccgaat
       61 tcgtggtcct cagaattggt ttagggttgt ttcgcggttc ttcccgtatt ctgcctccga
      121 ggcccagtgg ggctggctgt gggccccctg tgctgtttga ggcgtgaaac tcacctcagg
      181 aaatgtatct gatccattag cgtggattca tattatttgg tgatggagaa tcttcagctg
      241 tgttaagtgt taccgtcagt ttcagagatg gcttctttgc aaaggaaagg gctgcaggca
      301 aggattctca cctctgaaga agaggagaaa ctgaaaagag accaaacttt ggtgtctgat
      361 tttaaacagc agaaattgga acaagaggct cagaaaaatt gggatctttt ttacaaaaga
      421 aatagcacta atttcttcaa agacagacac tggaccacca gagagtttga ggagctaaga
      481 tcatgtagag agtttgaaga tcaaaagtta acaatgcttg aagctggctg tggggttgga
      541 aactgtttat tcccactttt agaagaagat ccgaatatct ttgcctatgc ctgtgatttt
      601 tctccaagag ccattgaata tgttaagcaa aatcctttat atgatacaga aagatgcaag
      661 gtattccagt gtgatctgac taaagatgat cttctggatc atgtaccgcc agagtctgtg
      721 gatgttgtta tgttgatatt tgtgctgtca gctgttcatc ctgataagat gcaccttgtc
      781 ttacaaaaca tttacaaggt attaaaacca ggcaaaagtg tcttgtttcg tgactacgga
      841 ctgtatgatc atgccatgct taggtttaaa gccagcagca aacttggaga aaacttttat
      901 gttagacaag atgggaccag atcatatttt tttactgatg acttcctggc tcagctcttt
      961 atggacacag gttatgaaga agtggtaaac gagtatgtgt ttcgagagac ggtgaataaa
     1021 aaagaaggcc tgtgtgtgcc aagagttttc cttcagagca aatttctaaa gcctcctaag
     1081 aacccatctc ctgtggtcct gggcctggat cctaagtcct gacctttcat gaggttggca
     1141 tttatacctt cccttgaaga ggaaaattta aaatcactct ttcgtctggg tgcggtggct
     1201 catgcctgta atcccagcac ttcgggaggc cgaggttggg ggatcatctg aggtcaggag
     1261 tttgagacca gcctggccaa catggtgaga ccccatctct actaaaaata caaaaaaatt
     1321 agctgggcat agtggcgagt gcctgtaatc ccagctactc aggaggctga ggcaagagaa
     1381 tcgcttgaac ccaagaggcg gagactgcag tgagccaaga tcatgccatt gcactccagc
     1441 ctgggcgaca gagtgagacc ctgtttcata aataaataaa atcactcttt ttactttata
     1501 tattttttga gacaggatct tgtggtacgc aggctggagt gcagtggtgt gtgatcacag
     1561 ctcattgtag cctcgacctc ccctgggctc aagcagtctt cctgcctcag cctcctgagt
     1621 agctaggact acaggtgtaa gccaccatgc ctggccctta atttttttta atgagcatgt
     1681 acagtagtcc cgctttatcc atggtttcac ttaccacaat ttcaattacc tgttttcaac
     1741 tgcagtcagt tacttttctt aagatatttt gagggttata ggctcatgcc tataacccca
     1801 gcactttgag aggccaaggc cagcagatcc cttgagccca ggagttcaag accagtgtgg
     1861 gcaacatggt gaaactctgt ctctacagaa aatacaaaaa ttagccaaat gtggtggcat
     1921 gtgcctgtag tcccagctgg ggtgggaggt tagcttgagc ctggaaggtg gaggttgcag
     1981 taagtcgaga tcacgctact gcactccagc ctgggcgaca gagcaaaagt ctgtctcaaa
     2041 aaaaacaaaa aaaatttgaa agatcacatt cacataactt ttattacggt atattgttaa
     2101 agttgttctt ttttttttag agtttggtac catcttggtt tcaggcaacc actgggggtc
     2161 ttgaaacata ccacccgcag atacgggggg ctactatgta ctttttgtat taaaaaatag
     2221 gaagtgtatg tgtgtgtgtt agagacatgt aaataaaatt tcaaggcctg aaaagtagtc
     2281 aaagttttat agaataacat aatttagcca tattgttcac tgtaaaattc cacaaagaaa
     2341 agcctaagga ccctgtagca cgtaaagaga ttcttgtcat tactcatgtg actgtaagtg
     2401 ttcccccagg caacatgtca gacaaccatg gggaaaaatg aggccaagta gactaaaata
     2461 agacactcat atatatttct agcctaaagg agtgttctgt tttcttcttt ttcgagtgtc
     2521 atatttgccc attggtctcc cctttagttc agttatgtat ttcttcaaaa ccagtgttcc
     2581 acaaatacca cttttgctct gtctgtgctc aagaaccc
//
