LOCUS       NM_022840               3684 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens methyltransferase 4, N6-adenosine (METTL4), transcript
            variant 1, mRNA.
ACCESSION   NM_022840
VERSION     NM_022840.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Liu,J., Zhang,N., Zeng,J., Wang,T., Shen,Y., Ma,C. and Yang,M.
  TITLE     N6 -methyladenosine-modified lncRNA ARHGAP5-AS1 stabilises CSDE1
            and coordinates oncogenic RNA regulons in hepatocellular carcinoma
  JOURNAL   Clin Transl Med 12 (11), e1107 (2022)
   PUBMED   36354136
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   van den Homberg,D.A.L., van der Kwast,R.V.C.T., Quax,P.H.A. and
            Nossent,A.Y.
  TITLE     N-6-Methyladenosine in Vasoactive microRNAs during Hypoxia; A Novel
            Role for METTL4
  JOURNAL   Int J Mol Sci 23 (3), 1057 (2022)
   PUBMED   35162982
  REMARK    GeneRIF: N-6-Methyladenosine in Vasoactive microRNAs during
            Hypoxia; A Novel Role for METTL4.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3684)
  AUTHORS   Goh,Y.T., Koh,C.W.Q., Sim,D.Y., Roca,X. and Goh,W.S.S.
  TITLE     METTL4 catalyzes m6Am methylation in U2 snRNA to regulate pre-mRNA
            splicing
  JOURNAL   Nucleic Acids Res 48 (16), 9250-9261 (2020)
   PUBMED   32813009
  REMARK    GeneRIF: METTL4 catalyzes m6Am methylation in U2 snRNA to regulate
            pre-mRNA splicing.
            GeneRIF: METTL4 methylates U2 snRNA Am at position 30 to generate
            m6Am in vivo. 'HMAGKD', where H = A/C/U, M = A/C, K = G/U, D =
            A/G/U, and A is the methylation site, is METTL4's in vivo preferred
            target motif.
REFERENCE   4  (bases 1 to 3684)
  AUTHORS   Chen,H., Gu,L., Orellana,E.A., Wang,Y., Guo,J., Liu,Q., Wang,L.,
            Shen,Z., Wu,H., Gregory,R.I., Xing,Y. and Shi,Y.
  TITLE     METTL4 is an snRNA m6Am methyltransferase that regulates RNA
            splicing
  JOURNAL   Cell Res 30 (6), 544-547 (2020)
   PUBMED   31913360
  REMARK    GeneRIF: METTL4 is an snRNA m(6)Am methyltransferase that regulates
            RNA splicing.
REFERENCE   5  (bases 1 to 3684)
  AUTHORS   Hao,Z., Wu,T., Cui,X., Zhu,P., Tan,C., Dou,X., Hsu,K.W., Lin,Y.T.,
            Peng,P.H., Zhang,L.S., Gao,Y., Hu,L., Sun,H.L., Zhu,A., Liu,J.,
            Wu,K.J. and He,C.
  TITLE     N6-Deoxyadenosine Methylation in Mammalian Mitochondrial DNA
  JOURNAL   Mol Cell 78 (3), 382-395 (2020)
   PUBMED   32183942
  REMARK    GeneRIF: N(6)-Deoxyadenosine Methylation in Mammalian Mitochondrial
            DNA.
REFERENCE   6  (bases 1 to 3684)
  AUTHORS   Kweon,S.M., Chen,Y., Moon,E., Kvederaviciute,K., Klimasauskas,S.
            and Feldman,D.E.
  TITLE     An Adversarial DNA N6-Methyladenine-Sensor Network Preserves
            Polycomb Silencing
  JOURNAL   Mol Cell 74 (6), 1138-1147 (2019)
   PUBMED   30982744
REFERENCE   7  (bases 1 to 3684)
  AUTHORS   Wang,P., Doxtader,K.A. and Nam,Y.
  TITLE     Structural Basis for Cooperative Function of Mettl3 and Mettl14
            Methyltransferases
  JOURNAL   Mol Cell 63 (2), 306-317 (2016)
   PUBMED   27373337
  REMARK    GeneRIF: For the methylation of adenosine in RNA, Mettl3 is the
            catalytically active subunit, while Mettl14 plays a structural role
            critical for substrate recognition.
REFERENCE   8  (bases 1 to 3684)
  AUTHORS   Cai,D.C., Fonteijn,H., Guadalupe,T., Zwiers,M., Wittfeld,K.,
            Teumer,A., Hoogman,M., Arias-Vasquez,A., Yang,Y., Buitelaar,J.,
            Fernandez,G., Brunner,H.G., van Bokhoven,H., Franke,B.,
            Hegenscheid,K., Homuth,G., Fisher,S.E., Grabe,H.J., Francks,C. and
            Hagoort,P.
  TITLE     A genome-wide search for quantitative trait loci affecting the
            cortical surface area and thickness of Heschl's gyrus
  JOURNAL   Genes Brain Behav 13 (7), 675-685 (2014)
   PUBMED   25130324
REFERENCE   9  (bases 1 to 3684)
  AUTHORS   Kobayashi,D., Nishizawa,D., Takasaki,Y., Kasai,S., Kakizawa,T.,
            Ikeda,K. and Fukuda,K.
  TITLE     Genome-wide association study of sensory disturbances in the
            inferior alveolar nerve after bilateral sagittal split ramus
            osteotomy
  JOURNAL   Mol Pain 9, 34 (2013)
   PUBMED   23834954
  REMARK    GeneRIF: associations revealed between genetic polymorphisms
            located in the flanking region of the ARID1B genes and hypoesthesia
            Publication Status: Online-Only
REFERENCE   10 (bases 1 to 3684)
  AUTHORS   Oh,J.H., Yang,J.O., Hahn,Y., Kim,M.R., Byun,S.S., Jeon,Y.J.,
            Kim,J.M., Song,K.S., Noh,S.M., Kim,S., Yoo,H.S., Kim,Y.S. and
            Kim,N.S.
  TITLE     Transcriptome analysis of human gastric cancer
  JOURNAL   Mamm Genome 16 (12), 942-954 (2005)
   PUBMED   16341674
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AP005136.2, HY026088.1, BC111020.1 and BU741018.1.
            
            On Nov 22, 2018 this sequence version replaced NM_022840.4.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.135717.1,
                                           SRR1803612.133169.1 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000574538.2/ ENSP00000458290.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 AP005136.2         146959-146960       c
            3-19                HY026088.1         2-18
            20-1722             BC111020.1         1-1703
            1723-1723           AP005136.2         122956-122956       c
            1724-2196           BC111020.1         1705-2177
            2197-3098           AP005136.2         113571-114472       c
            3099-3684           BU741018.1         23-608              c
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18p11.32"
     gene            1..3684
                     /gene="METTL4"
                     /gene_synonym="HsT661"
                     /note="methyltransferase 4, N6-adenosine"
                     /db_xref="GeneID:64863"
                     /db_xref="HGNC:HGNC:24726"
                     /db_xref="MIM:619626"
     exon            1..357
                     /gene="METTL4"
                     /gene_synonym="HsT661"
                     /inference="alignment:Splign:2.1.0"
     exon            358..1191
                     /gene="METTL4"
                     /gene_synonym="HsT661"
                     /inference="alignment:Splign:2.1.0"
     CDS             796..2214
                     /gene="METTL4"
                     /gene_synonym="HsT661"
                     /EC_number="2.1.1.72"
                     /note="isoform 1 is encoded by transcript variant 1;
                     methyltransferase-like protein 4; snRNA
                     (2'-O-methyladenosine-N(6)-)-methyltransferase METTL4;
                     N(6)-adenine-specific methyltransferase METTL4;
                     N(6)-adenine-specific DNA methyltransferase METTL4;
                     methyltransferase like 4"
                     /codon_start=1
                     /product="N(6)-adenine-specific methyltransferase METTL4
                     isoform 1"
                     /protein_id="NP_073751.3"
                     /db_xref="CCDS:CCDS11826.1"
                     /db_xref="GeneID:64863"
                     /db_xref="HGNC:HGNC:24726"
                     /db_xref="MIM:619626"
                     /translation="MSVVHQLSAGWLLDHLSFINKINYQLHQHHEPCCRKKEFTTSVH
                     FESLQMDSVSSSGVCAAFIASDSSTKPENDDGGNYEMFTRKFVFRPELFDVTKPYITP
                     AVHKECQQSNEKEDLMNGVKKEISISIIGKKRKRCVVFNQGELDAMEYHTKIRELILD
                     GSLQLIQEGLKSGFLYPLFEKQDKGSKPITLPLDACSLSELCEMAKHLPSLNEMEHQT
                     LQLVEEDTSVTEQDLFLRVVENNSSFTKVITLMGQKYLLPPKSSFLLSDISCMQPLLN
                     YRKTFDVIVIDPPWQNKSVKRSNRYSYLSPLQIQQIPIPKLAAPNCLLVTWVTNRQKH
                     LRFIKEELYPSWSVEVVAEWHWVKITNSGEFVFPLDSPHKKPYEGLILGRVQEKTALP
                     LRNADVNVLPIPDHKLIVSVPCTLHSHKPPLAEVLKDYIKPDGEYLELFARNLQPGWT
                     SWGNEVLKFQHVDYFIAVESGS"
     exon            1192..1254
                     /gene="METTL4"
                     /gene_synonym="HsT661"
                     /inference="alignment:Splign:2.1.0"
     exon            1255..1624
                     /gene="METTL4"
                     /gene_synonym="HsT661"
                     /inference="alignment:Splign:2.1.0"
     exon            1625..1694
                     /gene="METTL4"
                     /gene_synonym="HsT661"
                     /inference="alignment:Splign:2.1.0"
     exon            1695..1869
                     /gene="METTL4"
                     /gene_synonym="HsT661"
                     /inference="alignment:Splign:2.1.0"
     exon            1870..1976
                     /gene="METTL4"
                     /gene_synonym="HsT661"
                     /inference="alignment:Splign:2.1.0"
     exon            1977..2068
                     /gene="METTL4"
                     /gene_synonym="HsT661"
                     /inference="alignment:Splign:2.1.0"
     exon            2069..3684
                     /gene="METTL4"
                     /gene_synonym="HsT661"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3054..3059
                     /regulatory_class="polyA_signal_sequence"
                     /gene="METTL4"
                     /gene_synonym="HsT661"
                     /note="hexamer: AATAAA"
     polyA_site      3079
                     /gene="METTL4"
                     /gene_synonym="HsT661"
                     /note="major polyA site"
     regulatory      3658..3663
                     /regulatory_class="polyA_signal_sequence"
                     /gene="METTL4"
                     /gene_synonym="HsT661"
                     /note="hexamer: ATTAAA"
     polyA_site      3684
                     /gene="METTL4"
                     /gene_synonym="HsT661"
                     /note="major polyA site"
ORIGIN      
        1 ccatgcgacc gcctcgtcgc tggaaggctg cgtgctggtc gcgcccagct gcgtcacccc
       61 aggaactggg gtctgtgggc cagtgtggcc gtctctacga agactggcac gacccctaaa
      121 gttaggtcgg aagacctgtg ggcagcttga gcgccgagga gtgccctgaa cgctcaactc
      181 gccctggaaa cgtttttccg tacagcaaca tggcggcgcc catggactct tagaaaagga
      241 gaaagctttt tctctgtgga ctggaagggg catttttcat gatcactatt tagatgggtg
      301 ctgttttcat gaggagagtc tgggaaggcg gcgtccgctt ttctgacaag ggaagaggct
      361 actttgtcct tttaaggatt caatgacttc ctgacttgga ggatgtggac ctagtggcta
      421 gacccaagga ccaaagcaag aagtcgtggg gggcccagga agacaggagg atcacattgg
      481 gattccagac ataagatcag gttttaaccc cctttggcca aattttggct gaaaatgttg
      541 aattatcaac tctgaaatta aaaagaaagt ttatattaaa acattgcaat tttccttaga
      601 atttctgtat atattaacat catgaatgat aaattctctt caatgtgcat gtcaggtttt
      661 tgtacttgta tatcaaatct atctgtgtgt atgaagtgta tgtttattga aatacaagat
      721 atttaagaag ctgatctgga aagttggatt ttcattctag ttcctaattc ccagaggctt
      781 ttttaaagga agggaatgtc tgtggtacac cagttgtcag ctgggtggtt actggatcat
      841 ctttctttta tcaacaagat aaactatcaa cttcaccagc atcatgaacc ttgttgccgt
      901 aaaaaggagt tcactacttc tgttcacttt gagtctcttc aaatggattc tgtgtcctcc
      961 tctggagtct gtgctgcatt tattgcttct gactcttcca ctaagccaga gaatgatgat
     1021 ggaggaaatt atgaaatgtt cacacgaaaa tttgtttttc gacctgaact gtttgatgtc
     1081 accaaacctt atataactcc agctgttcat aaagaatgcc agcaaagtaa tgaaaaggaa
     1141 gatctgatga atggtgttaa aaaagaaatc tccatttcta ttattgggaa gaagcgtaaa
     1201 agatgtgttg ttttcaatca aggtgaattg gatgctatgg aataccatac aaagatcagg
     1261 gagctgattt tggatggatc tttacagttg atccaggaag gtctcaaaag tggttttctt
     1321 tatccacttt ttgaaaaaca ggacaagggt agtaagccca ttactttacc acttgacgcc
     1381 tgcagtttgt cagaattatg tgaaatggca aagcatttgc cttctctgaa tgaaatggaa
     1441 catcagacat tacaattggt ggaagaggat acatctgtta cagaacagga tttatttttg
     1501 cgagttgttg aaaacaactc tagctttaca aaagtgatta ctttaatggg acagaaatac
     1561 ctgctaccac cgaaaagcag ttttctttta tctgacattt cttgtatgca accacttcta
     1621 aactatagga aaacatttga tgtaattgtg atagatccac catggcagaa caaatcagtt
     1681 aaaagaagta ataggtacag ttatttgtca cccctgcaaa tacagcaaat acctatccct
     1741 aaattggctg ctccaaactg tcttcttgtt acttgggtga ccaatagaca gaagcaccta
     1801 cgttttataa aggaagaact ttatccctct tggtctgtgg aggtagttgc tgagtggcac
     1861 tgggtaaaaa taaccaattc aggagaattt gtgttcccat tagattctcc acacaaaaag
     1921 ccctacgaag gtcttatact ggggagggtt caagaaaaaa ctgctctacc attgaggaat
     1981 gcagatgtaa acgtgctccc cattccagac cacaaattaa ttgtcagcgt gccctgtact
     2041 cttcactcac ataagccacc gcttgctgag gttttaaaag actacatcaa gccagatggg
     2101 gaatatttgg agttgtttgc tcgaaattta cagccaggtt ggactagttg gggcaatgaa
     2161 gttctcaaat ttcagcatgt ggattatttt attgctgtgg agtctggaag ctgactatga
     2221 tcttgattaa agtagtggtt tcttcattgt ttcctcacca cttttccctt aattctaagt
     2281 cattttttta ttttgttacc aacccatatt cttagaatat aaacaggact tgtttttttc
     2341 agtaagggac cagaagtgac tagccttcat gtaattttaa gatgaatttt acttgagttg
     2401 cactaacatt ctatgttatt ctagactata caaattaagt ggtaagcagt tataaagacg
     2461 gcaagaccat gctattgaaa aagttcagaa aacatacacc gtggaccaga ggtcttaatc
     2521 ctatctatgg atgtgttttg tgtgacccat acagtgttgt aaaaaacact tagaaccatt
     2581 attctaaaaa atggggctat ttcacattaa agtccagatt tctgcttctt tttaaacatc
     2641 agaggctctg gctacacaga ggcctttgtt ctttcctggc atcagtctgc aggaccaagc
     2701 ggtggtggct cacttgggaa gagccttgtg ctctccactt tgccacagta ccactgccac
     2761 catgctgctc acttatgtca tccacttggc ccttgtatga cctgaatttg caacctctgg
     2821 tatactgtta tgttctggag aaaatattca aagatctgcc aaatactgca ttagtatact
     2881 gagtttatac agcatttttg tagggtttta aattgcattc aaggtcactt tccaagcact
     2941 ttctggtttt gcttgttttt ctagaagaaa atgaaaagct attccttata ataaacatgg
     3001 cagcaagtaa acagtgtgat tgtgaaaaaa atattattta tagattttct acaaataaat
     3061 atttgtctac caagtaaaat attttgactg aaatgattct ttgaaatgca tattgattta
     3121 ttatgtattg actttttaaa aattgaggta taattttcac aaaattctcc aattttcagt
     3181 gtcaaattca gtgaattttg aaaacatata tacagttgtc tgtctgccac agtgatcatg
     3241 atacagaaca ctttctttac cctgaaaact tctcattttt ccttttgcag tcaatcccct
     3301 gctcctatcc ttggcccctg gcaaacactg gtttgctttc tatcattagt tctgctgttt
     3361 gagaatttca tataaatgga atcatgcaat gtgtaatcta ttgtgcctgg cttctttcac
     3421 gtagcatttt gagaaaagca tttatactat ttacagattg ttgacaaata tttatccact
     3481 aagtaaaatg ttagactgaa atgattcttt gacaagcttg ccaatttact gattttgtca
     3541 aagaaaaata tgttattttt gaagtttgtt catcctttga gtgtgtgagt atagtatcag
     3601 aggcttaatt ttgtatttat ggagctattc taacttgtta tttaaaagga aaaaggtatt
     3661 aaacttgaag caaacttctc atga
//
