LOCUS       NM_019852               1980 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens methyltransferase 3, N6-adenosine-methyltransferase
            complex catalytic subunit (METTL3), mRNA.
ACCESSION   NM_019852
VERSION     NM_019852.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Xu,J., Sivakumar,C., Ryan,C.W. and Rao,R.C.
  TITLE     A novel interaction between RNA m6A methyltransferase METTL3 and
            RREB1
  JOURNAL   Biochem Biophys Res Commun 733, 150668 (2024)
   PUBMED   39278095
  REMARK    GeneRIF: A novel interaction between RNA m[6]A methyltransferase
            METTL3 and RREB1.
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Cui,Y., Pu,M., Gong,Y., Li,R., Wang,X., Ye,J., Huang,H., Liao,D.,
            Yang,Y., Yin,A., Li,J., Deng,Y., Tian,Z. and Pu,R.
  TITLE     METTL3-driven m6A modification of lncRNA FAM230B suppresses
            ferroptosis by modulating miR-27a-5p/BTF3 axis in gastric cancer
  JOURNAL   Biochim Biophys Acta Gen Subj 1868 (11), 130714 (2024)
   PUBMED   39278369
  REMARK    GeneRIF: METTL3-driven m6A modification of lncRNA FAM230B
            suppresses ferroptosis by modulating miR-27a-5p/BTF3 axis in
            gastric cancer.
REFERENCE   3  (bases 1 to 1980)
  AUTHORS   Yu,Z., Xia,Y., Li,J., Jiang,J., Li,Y., Li,Y. and Wang,L.
  TITLE     METTL3 mediates m6A modification of lncRNA CRNDE to promote ATG10
            expression and improve brain ischemia/reperfusion injury through
            YTHDC1
  JOURNAL   Biol Direct 19 (1), 92 (2024)
   PUBMED   39407279
  REMARK    GeneRIF: METTL3 mediates m6A modification of lncRNA CRNDE to
            promote ATG10 expression and improve brain ischemia/reperfusion
            injury through YTHDC1.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1980)
  AUTHORS   Zhang,M., Gou,Z., Qu,Y. and Su,X.
  TITLE     The indispensability of methyltransferase-like 3 in the immune
            system: from maintaining homeostasis to driving function
  JOURNAL   Front Immunol 15, 1456891 (2024)
   PUBMED   39416774
  REMARK    GeneRIF: The indispensability of methyltransferase-like 3 in the
            immune system: from maintaining homeostasis to driving function.
            Review article
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1980)
  AUTHORS   Ma,Y., Shi,H. and Zheng,W.
  TITLE     METTL3 Regulates the Translation of Oncogene Myc through m6A
            Modification and Promotes the Occurrence and Development of
            Cervical Cancer
  JOURNAL   Discov Med 36 (188), 1902-1910 (2024)
   PUBMED   39327253
  REMARK    GeneRIF: METTL3 Regulates the Translation of Oncogene Myc through
            m[6]A Modification and Promotes the Occurrence and Development of
            Cervical Cancer.
REFERENCE   6  (bases 1 to 1980)
  AUTHORS   Beausoleil,S.A., Villen,J., Gerber,S.A., Rush,J. and Gygi,S.P.
  TITLE     A probability-based approach for high-throughput protein
            phosphorylation analysis and site localization
  JOURNAL   Nat Biotechnol 24 (10), 1285-1292 (2006)
   PUBMED   16964243
REFERENCE   7  (bases 1 to 1980)
  AUTHORS   Heilig,R., Eckenberg,R., Petit,J.L., Fonknechten,N., Da Silva,C.,
            Cattolico,L., Levy,M., Barbe,V., de Berardinis,V., Ureta-Vidal,A.,
            Pelletier,E., Vico,V., Anthouard,V., Rowen,L., Madan,A., Qin,S.,
            Sun,H., Du,H., Pepin,K., Artiguenave,F., Robert,C., Cruaud,C.,
            Bruls,T., Jaillon,O., Friedlander,L., Samson,G., Brottier,P.,
            Cure,S., Segurens,B., Aniere,F., Samain,S., Crespeau,H., Abbasi,N.,
            Aiach,N., Boscus,D., Dickhoff,R., Dors,M., Dubois,I., Friedman,C.,
            Gouyvenoux,M., James,R., Madan,A., Mairey-Estrada,B., Mangenot,S.,
            Martins,N., Menard,M., Oztas,S., Ratcliffe,A., Shaffer,T.,
            Trask,B., Vacherie,B., Bellemere,C., Belser,C., Besnard-Gonnet,M.,
            Bartol-Mavel,D., Boutard,M., Briez-Silla,S., Combette,S.,
            Dufosse-Laurent,V., Ferron,C., Lechaplais,C., Louesse,C.,
            Muselet,D., Magdelenat,G., Pateau,E., Petit,E.,
            Sirvain-Trukniewicz,P., Trybou,A., Vega-Czarny,N., Bataille,E.,
            Bluet,E., Bordelais,I., Dubois,M., Dumont,C., Guerin,T.,
            Haffray,S., Hammadi,R., Muanga,J., Pellouin,V., Robert,D.,
            Wunderle,E., Gauguet,G., Roy,A., Sainte-Marthe,L., Verdier,J.,
            Verdier-Discala,C., Hillier,L., Fulton,L., McPherson,J.,
            Matsuda,F., Wilson,R., Scarpelli,C., Gyapay,G., Wincker,P.,
            Saurin,W., Quetier,F., Waterston,R., Hood,L. and Weissenbach,J.
  TITLE     The DNA sequence and analysis of human chromosome 14
  JOURNAL   Nature 421 (6923), 601-607 (2003)
   PUBMED   12508121
  REMARK    Erratum:[Nature. 2023 Aug;620(7973):E17. doi:
            10.1038/s41586-023-06403-y.. PMID: 37491470]
REFERENCE   8  (bases 1 to 1980)
  AUTHORS   Bujnicki,J.M., Feder,M., Radlinska,M. and Blumenthal,R.M.
  TITLE     Structure prediction and phylogenetic analysis of a functionally
            diverse family of proteins homologous to the MT-A70 subunit of the
            human mRNA:m(6)A methyltransferase
  JOURNAL   J Mol Evol 55 (4), 431-444 (2002)
   PUBMED   12355263
REFERENCE   9  (bases 1 to 1980)
  AUTHORS   Leach,R.A. and Tuck,M.T.
  TITLE     Expression of the mRNA (N6-adenosine)-methyltransferase
            S-adenosyl-L-methionine binding subunit mRNA in cultured cells
  JOURNAL   Int J Biochem Cell Biol 33 (10), 984-999 (2001)
   PUBMED   11470232
REFERENCE   10 (bases 1 to 1980)
  AUTHORS   Bokar,J.A., Shambaugh,M.E., Polayes,D., Matera,A.G. and
            Rottman,F.M.
  TITLE     Purification and cDNA cloning of the AdoMet-binding subunit of the
            human mRNA (N6-adenosine)-methyltransferase
  JOURNAL   RNA 3 (11), 1233-1247 (1997)
   PUBMED   9409616
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC295053.1, BC001650.2,
            AL161747.5 and CA311761.1.
            
            On Nov 23, 2018 this sequence version replaced NM_019852.4.
            
            Summary: This gene encodes the 70 kDa subunit of MT-A which is part
            of N6-adenosine-methyltransferase. This enzyme is involved in the
            posttranscriptional methylation of internal adenosine residues in
            eukaryotic mRNAs, forming N6-methyladenosine. [provided by RefSeq,
            Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC052244.1, AK000222.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000298717.9/ ENSP00000298717.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-31                DC295053.1         36-66
            32-1451             BC001650.2         1-1420
            1452-1452           AL161747.5         116009-116009       c
            1453-1973           BC001650.2         1422-1942
            1974-1980           CA311761.1         24-30               c
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q11.2"
     gene            1..1980
                     /gene="METTL3"
                     /gene_synonym="hMETTL3; IME4; M6A; MT-A70; Spo8"
                     /note="methyltransferase 3, N6-adenosine-methyltransferase
                     complex catalytic subunit"
                     /db_xref="GeneID:56339"
                     /db_xref="HGNC:HGNC:17563"
                     /db_xref="MIM:612472"
     exon            1..217
                     /gene="METTL3"
                     /gene_synonym="hMETTL3; IME4; M6A; MT-A70; Spo8"
                     /inference="alignment:Splign:2.1.0"
     CDS             118..1860
                     /gene="METTL3"
                     /gene_synonym="hMETTL3; IME4; M6A; MT-A70; Spo8"
                     /EC_number="2.1.1.348"
                     /note="adoMet-binding subunit of the human mRNA
                     (N6-adenosine)-methyltransferase; mRNA m(6)A
                     methyltransferase; N6-adenosine-methyltransferase 70 kDa
                     subunit; methyltransferase-like protein 3; mRNA
                     (2'-O-methyladenosine-N(6)-)-methyltransferase;
                     methyltransferase like 3; N6-adenosine-methyltransferase
                     catalytic subunit; N(6)-adenosine-methyltransferase 70 kDa
                     subunit"
                     /codon_start=1
                     /product="N(6)-adenosine-methyltransferase catalytic
                     subunit METTL3"
                     /protein_id="NP_062826.2"
                     /db_xref="CCDS:CCDS32044.1"
                     /db_xref="GeneID:56339"
                     /db_xref="HGNC:HGNC:17563"
                     /db_xref="MIM:612472"
                     /translation="MSDTWSSIQAHKKQLDSLRERLQRRRKQDSGHLDLRNPEAALSP
                     TFRSDSPVPTAPTSGGPKPSTASAVPELATDPELEKKLLHHLSDLALTLPTDAVSICL
                     AISTPDAPATQDGVESLLQKFAAQELIEVKRGLLQDDAHPTLVTYADHSKLSAMMGAV
                     AEKKGPGEVAGTVTGQKRRAEQDSTTVAAFASSLVSGLNSSASEPAKEPAKKSRKHAA
                     SDVDLEIESLLNQQSTKEQQSKKVSQEILELLNTTTAKEQSIVEKFRSRGRAQVQEFC
                     DYGTKEECMKASDADRPCRKLHFRRIINKHTDESLGDCSFLNTCFHMDTCKYVHYEID
                     ACMDSEAPGSKDHTPSQELALTQSVGGDSSADRLFPPQWICCDIRYLDVSILGKFAVV
                     MADPPWDIHMELPYGTLTDDEMRRLNIPVLQDDGFLFLWVTGRAMELGRECLNLWGYE
                     RVDEIIWVKTNQLQRIIRTGRTGHWLNHGKEHCLVGVKGNPQGFNQGLDCDVIVAEVR
                     STSHKPDEIYGMIERLSPGTRKIELFGRPHNVQPNWITLGNQLDGIHLLDPDVVARFK
                     QRYPDGIISKPKNL"
     exon            218..435
                     /gene="METTL3"
                     /gene_synonym="hMETTL3; IME4; M6A; MT-A70; Spo8"
                     /inference="alignment:Splign:2.1.0"
     exon            436..840
                     /gene="METTL3"
                     /gene_synonym="hMETTL3; IME4; M6A; MT-A70; Spo8"
                     /inference="alignment:Splign:2.1.0"
     exon            841..1016
                     /gene="METTL3"
                     /gene_synonym="hMETTL3; IME4; M6A; MT-A70; Spo8"
                     /inference="alignment:Splign:2.1.0"
     exon            1017..1233
                     /gene="METTL3"
                     /gene_synonym="hMETTL3; IME4; M6A; MT-A70; Spo8"
                     /inference="alignment:Splign:2.1.0"
     exon            1234..1421
                     /gene="METTL3"
                     /gene_synonym="hMETTL3; IME4; M6A; MT-A70; Spo8"
                     /inference="alignment:Splign:2.1.0"
     exon            1422..1460
                     /gene="METTL3"
                     /gene_synonym="hMETTL3; IME4; M6A; MT-A70; Spo8"
                     /inference="alignment:Splign:2.1.0"
     exon            1461..1569
                     /gene="METTL3"
                     /gene_synonym="hMETTL3; IME4; M6A; MT-A70; Spo8"
                     /inference="alignment:Splign:2.1.0"
     exon            1570..1635
                     /gene="METTL3"
                     /gene_synonym="hMETTL3; IME4; M6A; MT-A70; Spo8"
                     /inference="alignment:Splign:2.1.0"
     exon            1636..1748
                     /gene="METTL3"
                     /gene_synonym="hMETTL3; IME4; M6A; MT-A70; Spo8"
                     /inference="alignment:Splign:2.1.0"
     exon            1749..1980
                     /gene="METTL3"
                     /gene_synonym="hMETTL3; IME4; M6A; MT-A70; Spo8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1955..1960
                     /regulatory_class="polyA_signal_sequence"
                     /gene="METTL3"
                     /gene_synonym="hMETTL3; IME4; M6A; MT-A70; Spo8"
                     /note="hexamer: AATAAA"
     polyA_site      1980
                     /gene="METTL3"
                     /gene_synonym="hMETTL3; IME4; M6A; MT-A70; Spo8"
                     /note="major polyA site"
ORIGIN      
        1 attttccggt tagccttcgg ggtgtccgcg tgagaattgg ctatatcctg gagcgagtgc
       61 tgggaggtgc tagtccgccg cgccttattc gagaggtgtc agggctggga gactaggatg
      121 tcggacacgt ggagctctat ccaggcccac aagaagcagc tggactctct gcgggagagg
      181 ctgcagcgga ggcggaagca ggactcgggg cacttggatc tacggaatcc agaggcagca
      241 ttgtctccaa ccttccgtag tgacagccca gtgcctactg cacccacctc tggtggccct
      301 aagcccagca cagcttcagc agttcctgaa ttagctacag atcctgagtt agagaagaag
      361 ttgctacacc acctctctga tctggcctta acattgccca ctgatgctgt gtccatctgt
      421 cttgccatct ccacgccaga tgctcctgcc actcaagatg gggtagaaag cctcctgcag
      481 aagtttgcag ctcaggagtt gattgaggta aagcgaggtc tcctacaaga tgatgcacat
      541 cctactcttg taacctatgc tgaccattcc aagctctctg ccatgatggg tgctgtggca
      601 gaaaagaagg gccctgggga ggtagcaggg actgtcacag ggcagaagcg gcgtgcagaa
      661 caggactcga ctacagtagc tgcctttgcc agttcgttag tctctggtct gaactcttca
      721 gcatcggaac cagcaaagga gccagccaag aaatcaagga aacatgctgc ctcagatgtt
      781 gatctggaga tagagagcct tctgaaccaa cagtccacta aggaacaaca gagcaagaag
      841 gtcagtcagg agatcctaga gctattaaat actacaacag ccaaggaaca atccattgtt
      901 gaaaaatttc gctctcgagg tcgggcccaa gtgcaagaat tctgtgacta tggaaccaag
      961 gaggagtgca tgaaagccag tgatgctgat cgaccctgtc gcaagctgca cttcagacga
     1021 attatcaata aacacactga tgagtcttta ggtgactgct ctttccttaa tacatgtttc
     1081 cacatggata cctgcaagta tgttcactat gaaattgatg cttgcatgga ttctgaggcc
     1141 cctggcagca aagaccacac gccaagccag gagcttgctc ttacacagag tgtcggaggt
     1201 gattccagtg cagaccgact cttcccacct cagtggatct gttgtgatat ccgctacctg
     1261 gacgtcagta tcttgggcaa gtttgcagtt gtgatggctg acccaccctg ggatattcac
     1321 atggaactgc cctatgggac cctgacagat gatgagatgc gcaggctcaa catacccgta
     1381 ctacaggatg atggctttct cttcctctgg gtcacaggca gggccatgga gttggggaga
     1441 gaatgtctaa acctctgggg gtatgaacgg gtagatgaaa ttatttgggt gaagacaaat
     1501 caactgcaac gcatcattcg gacaggccgt acaggtcact ggttgaacca tgggaaggaa
     1561 cactgcttgg ttggtgtcaa aggaaatccc caaggcttca accagggtct ggattgtgat
     1621 gtgatcgtag ctgaggttcg ttccaccagt cataaaccag atgaaatcta tggcatgatt
     1681 gaaagactat ctcctggcac tcgcaagatt gagttatttg gacgaccaca caatgtgcaa
     1741 cccaactgga tcacccttgg aaaccaactg gatgggatcc acctactaga cccagatgtg
     1801 gttgcacggt tcaagcaaag gtacccagat ggtatcatct ctaaacctaa gaatttatag
     1861 aagcacttcc ttacagagct aagaatccat agccatggct ctgtaagcta aacctgaaga
     1921 gtgatatttg tacaatagct ttcttcttta tttaaataaa catttgtatt gtagttggga
//
