LOCUS       NM_018396               5841 bp    mRNA    linear   PRI 08-DEC-2024
DEFINITION  Homo sapiens methyltransferase 2B, tRNA N3-cytidine (METTL2B),
            mRNA.
ACCESSION   NM_018396
VERSION     NM_018396.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Mao,X.L., Li,Z.H., Huang,M.H., Wang,J.T., Zhou,J.B., Li,Q.R.,
            Xu,H., Wang,X.J. and Zhou,X.L.
  TITLE     Mutually exclusive substrate selection strategy by human m3C RNA
            transferases METTL2A and METTL6
  JOURNAL   Nucleic Acids Res 49 (14), 8309-8323 (2021)
   PUBMED   34268557
  REMARK    GeneRIF: Mutually exclusive substrate selection strategy by human
            m3C RNA transferases METTL2A and METTL6.
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Lentini,J.M., Alsaif,H.S., Faqeih,E., Alkuraya,F.S. and Fu,D.
  TITLE     DALRD3 encodes a protein mutated in epileptic encephalopathy that
            targets arginine tRNAs for 3-methylcytosine modification
  JOURNAL   Nat Commun 11 (1), 2510 (2020)
   PUBMED   32427860
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5841)
  AUTHORS   Xu,L., Liu,X., Sheng,N., Oo,K.S., Liang,J., Chionh,Y.H., Xu,J.,
            Ye,F., Gao,Y.G., Dedon,P.C. and Fu,X.Y.
  TITLE     Three distinct 3-methylcytidine (m3C) methyltransferases modify
            tRNA and mRNA in mice and humans
  JOURNAL   J Biol Chem 292 (35), 14695-14703 (2017)
   PUBMED   28655767
REFERENCE   4  (bases 1 to 5841)
  AUTHORS   Noma,A., Yi,S., Katoh,T., Takai,Y., Suzuki,T. and Suzuki,T.
  TITLE     Actin-binding protein ABP140 is a methyltransferase for
            3-methylcytidine at position 32 of tRNAs in Saccharomyces
            cerevisiae
  JOURNAL   RNA 17 (6), 1111-1119 (2011)
   PUBMED   21518805
REFERENCE   5  (bases 1 to 5841)
  AUTHORS   Zody,M.C., Garber,M., Adams,D.J., Sharpe,T., Harrow,J.,
            Lupski,J.R., Nicholson,C., Searle,S.M., Wilming,L., Young,S.K.,
            Abouelleil,A., Allen,N.R., Bi,W., Bloom,T., Borowsky,M.L.,
            Bugalter,B.E., Butler,J., Chang,J.L., Chen,C.K., Cook,A., Corum,B.,
            Cuomo,C.A., de Jong,P.J., DeCaprio,D., Dewar,K., FitzGerald,M.,
            Gilbert,J., Gibson,R., Gnerre,S., Goldstein,S., Grafham,D.V.,
            Grocock,R., Hafez,N., Hagopian,D.S., Hart,E., Norman,C.H.,
            Humphray,S., Jaffe,D.B., Jones,M., Kamal,M., Khodiyar,V.K.,
            LaButti,K., Laird,G., Lehoczky,J., Liu,X., Lokyitsang,T.,
            Loveland,J., Lui,A., Macdonald,P., Major,J.E., Matthews,L.,
            Mauceli,E., McCarroll,S.A., Mihalev,A.H., Mudge,J., Nguyen,C.,
            Nicol,R., O'Leary,S.B., Osoegawa,K., Schwartz,D.C., Shaw-Smith,C.,
            Stankiewicz,P., Steward,C., Swarbreck,D., Venkataraman,V.,
            Whittaker,C.A., Yang,X., Zimmer,A.R., Bradley,A., Hubbard,T.,
            Birren,B.W., Rogers,J., Lander,E.S. and Nusbaum,C.
  TITLE     DNA sequence of human chromosome 17 and analysis of rearrangement
            in the human lineage
  JOURNAL   Nature 440 (7087), 1045-1049 (2006)
   PUBMED   16625196
REFERENCE   6  (bases 1 to 5841)
  AUTHORS   Zhang,S.X., Guo,Y. and Boulianne,G.L.
  TITLE     Identification of a novel family of putative methyltransferases
            that interact with human and Drosophila presenilins
  JOURNAL   Gene 280 (1-2), 135-144 (2001)
   PUBMED   11738826
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP247182.1, AC010655.7,
            AC090114.5 and BC064929.1.
            
            On Dec 2, 2019 this sequence version replaced NM_018396.2.
            
            Summary: This gene is a member of a family of methyltransferases
            that share homology with, but are distinct from, the UbiE family of
            methyltransferases. Alternatively spliced variants which encode
            different protein isoforms have been described; however, not all
            variants have been fully characterized. [provided by RefSeq, Jul
            2008].
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK302775.1, SRR11853567.27932.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000262432.13/ ENSP00000262432.9
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-79                BP247182.1         20-98
            80-128              AC010655.7         146576-146624
            129-220             AC010655.7         146831-146922
            221-576             AC010655.7         148907-149262
            577-626             AC010655.7         150396-150445
            627-687             AC010655.7         157850-157910
            688-827             AC090114.5         463-602
            828-934             AC090114.5         4695-4801
            935-1000            AC090114.5         7562-7627
            1001-1485           AC090114.5         8421-8905
            1486-1773           AC090114.5         8906-9193
            1774-2163           BC064929.1         1754-2143
            2164-5841           AC090114.5         9584-13261
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q32.1"
     gene            1..5841
                     /gene="METTL2B"
                     /gene_synonym="METL; METTL2; METTL2A; PSENIP1"
                     /note="methyltransferase 2B, tRNA N3-cytidine"
                     /db_xref="GeneID:55798"
                     /db_xref="HGNC:HGNC:18272"
                     /db_xref="MIM:607846"
     exon            1..128
                     /gene="METTL2B"
                     /gene_synonym="METL; METTL2; METTL2A; PSENIP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             19..1155
                     /gene="METTL2B"
                     /gene_synonym="METL; METTL2; METTL2A; PSENIP1"
                     /note="methyltransferase-like protein 2B; tRNA
                     N(3)-methylcytidine methyltransferase METTL2B;
                     methyltransferase like 2B; methyltransferase 2B,
                     methylcytidine"
                     /codon_start=1
                     /product="tRNA N(3)-cytidine methyltransferase METTL2B"
                     /protein_id="NP_060866.2"
                     /db_xref="CCDS:CCDS5803.2"
                     /db_xref="GeneID:55798"
                     /db_xref="HGNC:HGNC:18272"
                     /db_xref="MIM:607846"
                     /translation="MAGSYPEGAPAILADKRQQFGSRFLSDPARVFHHNAWDNVEWSE
                     EQAAAAERKVQENSIQRVCQEKQVDYEINAHKYWNDFYKIHENGFFKDRHWLFTEFPE
                     LAPSQNQNHLKDWFLENKSEVCECRNNEDGPGLIMEEQHKCSSKSLEHKTQTPPVEEN
                     VTQKISDLEICADEFPGSSATYRILEVGCGVGNTVFPILQTNNDPGLFVYCCDFSSTA
                     IELVQTNSEYDPSRCFAFVHDLCDEEKSYPVPKGSLDIIILIFVLSAVVPDKMQKAIN
                     RLSRLLKPGGMVLLRDYGRYDMAQLRFKKGQCLSGNFYVRGDGTRVYFFTQEELDTLF
                     TTAGLEKVQNLVDRRLQVNRGKQLTMYRVWIQCKYCKPLLSSTS"
     exon            129..220
                     /gene="METTL2B"
                     /gene_synonym="METL; METTL2; METTL2A; PSENIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            221..576
                     /gene="METTL2B"
                     /gene_synonym="METL; METTL2; METTL2A; PSENIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            577..626
                     /gene="METTL2B"
                     /gene_synonym="METL; METTL2; METTL2A; PSENIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            627..687
                     /gene="METTL2B"
                     /gene_synonym="METL; METTL2; METTL2A; PSENIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            688..827
                     /gene="METTL2B"
                     /gene_synonym="METL; METTL2; METTL2A; PSENIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            828..934
                     /gene="METTL2B"
                     /gene_synonym="METL; METTL2; METTL2A; PSENIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            935..1000
                     /gene="METTL2B"
                     /gene_synonym="METL; METTL2; METTL2A; PSENIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1001..5841
                     /gene="METTL2B"
                     /gene_synonym="METL; METTL2; METTL2A; PSENIP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5819..5824
                     /regulatory_class="polyA_signal_sequence"
                     /gene="METTL2B"
                     /gene_synonym="METL; METTL2; METTL2A; PSENIP1"
                     /note="hexamer: AATAAA"
     polyA_site      5841
                     /gene="METTL2B"
                     /gene_synonym="METL; METTL2; METTL2A; PSENIP1"
                     /note="major polyA site"
ORIGIN      
        1 gtttccggct ccggtgtcat ggccggctcc taccctgaag gtgcacctgc aatcctcgcc
       61 gataagaggc agcagttcgg aagccggttc ctgagcgatc cggcgcgcgt cttccaccac
      121 aatgcctggg acaatgtgga gtggtcggaa gagcaagccg cggcggcgga gagaaaagtc
      181 caggagaaca gtatccagcg ggtgtgccag gagaaacaag ttgattatga gatcaatgcc
      241 cacaaatact ggaatgactt ctacaaaatc cacgaaaatg ggtttttcaa ggatagacat
      301 tggcttttta ccgaattccc tgagctggca cctagccaaa atcaaaatca tttgaaggat
      361 tggttcttgg agaacaagag tgaagtatgt gaatgtagaa acaatgagga tggacctggt
      421 ttaataatgg aagaacagca caagtgttct tcgaagagcc ttgaacataa aacacagaca
      481 cctcctgtgg aggagaatgt aactcagaaa attagtgacc tggaaatttg tgctgatgag
      541 tttcctggat cctcagccac ctaccgaata ctggaggttg gctgtggtgt gggaaacaca
      601 gtctttccaa ttttacaaac gaacaatgac ccaggactct ttgtttattg ctgtgatttt
      661 tcttccacag ctatagaact ggtccagaca aattcagaat atgatccttc tcggtgtttt
      721 gcctttgttc acgacctgtg tgatgaagag aagagttacc cagtgcccaa gggcagtctt
      781 gatattatca ttctcatatt tgttctttca gcagttgttc cagacaagat gcagaaggct
      841 atcaacaggc tgagcaggct tctgaaacct ggggggatgg tacttctgcg agattacggc
      901 cgctatgaca tggctcagct tcggtttaaa aaaggtcagt gtctatctgg aaatttctat
      961 gtgagaggtg atggaaccag agtttacttc ttcacacaag aggaactgga cacgcttttc
     1021 accactgctg gactggaaaa agttcagaat ctggtggacc gccgactgca ggtgaaccga
     1081 gggaagcaac tgacaatgta ccgggtttgg attcagtgca aatactgcaa gccccttctg
     1141 tccagcacca gctaagaggc acctgctgcc aacacgatgc aagcccgttg tgtttccgag
     1201 cttttttaaa aaaaaatttg tagcaccggg catggtgcat gcctgtaatc ccagccactc
     1261 aggaggctga ggcagggagg atccattgag cccaggagtc cagcctgggc aaaatagcga
     1321 gagaccctga atctgaaagt aatgataaaa taaaaagaat ataaatgagg tctcgttgat
     1381 gctggacaat tcaagaattc agacttgaac cttaaaccta ggaaaagtta ctttgtatca
     1441 ggattctaac aattatgctt catatttgtg aagtccttta aaacataatt ttctcaagtt
     1501 ctttctttga gacctcaatc tgtcttagca ttttgtaact aataactgaa attttattca
     1561 aaggaattgt aaaccttaaa ccaccattta tttccatgtg aaaaagtgtt atatatgaca
     1621 agtgtttttt gattgtaatt gcgttaaatc ttttgagagt gtaaatgccg ggctaggcaa
     1681 ttgcagttaa tacatacagg ggttagtgaa gggcttatta agttgtaggg gaagcaagct
     1741 gggaagaatc agatcagata ttttcctgac aagaaaaaaa tgaccctgta gacagcatca
     1801 aaatgtggtg ttcttgttaa gtaattgatc gtggtctttg ctttaatctt agttccgcca
     1861 ggcacggtgg ctcacacctg taatcccagc actttgggag gccgaggcgg gcagatcacc
     1921 tgaggtcagg agttcgagac cagcgtggcc aacatggtga aaccccgtct ctactaaaga
     1981 taaaaaaatt agctgggtgt gttggtgggt gcctgtaatc cagctactcg ggaggctgag
     2041 gcaggagaat cacttgaacc caggaggcag aggttgcagt gagctgagat tgcgccactg
     2101 tactccagcc tgggcaacaa agcaagactc tgtctcaaaa aaataaaaat aaaaaaaatc
     2161 ttagttccat ggaattttaa gcactgttcc ccctctaacc tatgtctaaa gaattaaaag
     2221 aatttggctg ggtgcggtag ctcatgcctg taatcccaac actttgggag gctgaggcag
     2281 gtggatcacg aggtcaggag atcgagacca tcctggctaa cacggtgaaa cctcgtctcc
     2341 actaaaaata ccaaaaaaaa tagctgggca tggtggcatg cgcctgtagt cccctgtagt
     2401 cccagctact cgggaggcta aggcaggaga atcgtttgaa cctgggaggc agaggttgca
     2461 gtgagccgag atcatgtcac tgcattccag cctgggcaac agaggaagac tctgtctcaa
     2521 aaaaataaaa gaaaagaatt ttcacttttt gcaaaattat ctttttttac tgcaccctta
     2581 agtgtttgac acaaaaggaa ttgagagaac ttcttaattt taaccacatc atcacttcac
     2641 ttctgaatgt gatcactgtc agtgttagat gctttttttt ttttttttta ggagacaggg
     2701 ctctcacttt gttgcccagg ctggagttca gtggtgcaat gatagctcat tatcacctcg
     2761 aacttctggc ttgagccatc gctccacctc agcctcctga gtagctagga ctgcaggtgg
     2821 gcaccatcat gcctagctaa ttgttagatc cttttttttt atgttctgca ttttttcaat
     2881 tatttttaca tatttctgtc ttgatccatg cagttatagg atatatcctt aattttagaa
     2941 agtaaggact tttcgaccag gcatggcggc tcacgcctat aatcccagca ctttgggagg
     3001 ctgaggtggg cagatcactt gaggtcagga gttccagtcc agcccggcta acatagtgaa
     3061 accccgtctc cactaaaaaa taaaaaatca gcccggcatg gttgtgggcg cctgtaatcc
     3121 cagctactca ggaggctgag gtaggagaat tgcttgaacc tgggaggcgg aggttgcagt
     3181 gagctgagat ggtgccattg cactccagcc tgggcgacaa gagcgaaact gcatctcaaa
     3241 aacaaagatg cctagctttc accttgttga tgtttttcta aattttggaa taataaattt
     3301 atataggaat actgcaagaa tagtccattt ttttctgatg tcccaacatc agcaaatata
     3361 ttaccaaata tatccatcac aagtaggaat acatggaaac attaatgcca gccactaacc
     3421 attcatgttt acccagtagt cccaataata gctttgtcac acaaaaacat gattatgttg
     3481 gctgggcaca gtggctcctg taatcttagc attttgggag cccaaggcag gaggatgact
     3541 tgaggccagg agttcaagac caggctgggt aatgtagcaa gaccctgact tttttttttt
     3601 ttttttttga gatggagttt cgctcttgtt gcccaggctg gagtacaatg gcacgatctc
     3661 ggctcactgc aatctccacc tcccaagttt aagtgattct cctgcctcag cctcccaagt
     3721 agctggaatt acaggcatgc agcaccattc ccagctaaat tttttgtatt tttagtagag
     3781 acaaggtttc tccatgttgg tcaggctgat ctcgaactcc cgacctcagg tgatctgccc
     3841 gcctcagcct cccaaagtgc tgggattaca ggcgtgagcc accacgcccg gccaagaccc
     3901 tgacttttta aaaaatgtta aacataggcc gggtgcagtg gctcacgcct gtaatcccag
     3961 cactttggga ggccgaggtg ggcggatcat ttgaggtcag gagttcgaga ccagcctggc
     4021 caacttaatg aaaccccatc tctgctaaca atacagacca ggcatggtgg ctcacacctg
     4081 taatcccagc actttgagag accgaggcag gtggatcacc tgaggtcggg agttcgagac
     4141 cagcctgacc aacatggaga aaccccgtct ctactaaaaa tacaaaatag ccaggtgtga
     4201 tggcgcatgc ctgtaacccc atctacttgg aaggctgagg caggagaatc acttgaatct
     4261 gggaggcaga ggttgcagtg agccgagatg gcgccattgc actccagcct gggcaacaag
     4321 agtgaaactc cgtctcaaaa aaaaaaaaaa aaaaaaatta gctgggcatg gtggtgggcg
     4381 cctgtaatcc cagctatctg ggaggctgag tctagagaat catttgaacc tgggaggcgg
     4441 aggttgcagt gagccgagat cgcaccattg cactccagcc tgggcaacag tgagactccg
     4501 tctcaaaata aaaaaacaaa aaaaatgtaa aaaataaata attgtattga atttattagt
     4561 tgtgtctcag tctccttcag tctacagttt aggcttgctt ttgcaccttt gcattttaag
     4621 gtaagaggct attgtgtaaa ctcgaattgg gtttgttggt gtttcttcat gatttggttc
     4681 aggctctgca ttttgggcag gaatattcag aggtgatgct gtgttcctcc cataggatcg
     4741 tatcaggcga cacatgatta tgaatttccc cattattggt gatgtcactt agttgtgttg
     4801 tctgccaggt tctgccactg taaagttaat aagtattttg tagggaagtg ctttgagact
     4861 aaatatcctg ttcctcatca aacttctacc ccctagtttc agtatctatt gctgtttttt
     4921 gtctgaataa ctatgatagt tgccaaatag tggtttctga tccttccatt ccttctacac
     4981 tgattggttg gtattttgta cagaaaagct ttctcatatc tgatttactt gtctcaatat
     5041 ggacccatgg gtatttgagt ttcttgtggg gttatttgtt gttgtttttg agacaggctc
     5101 acccaggctg gagtgcagca gcacgatcac tgcttactgc ttactgcagc ctcgacctcc
     5161 catgctcaag tgattctccc accccagcct cccaagtaga tgggactaca ggcatgtgcc
     5221 accaagccta atttttgtat tttttgtaga gacagggttt caccatgtgt cccaggctgg
     5281 tcttgagctc ctgagctcaa gcaatcctcc cacctcggcc tcccaaagtg ctgggatgag
     5341 ccaccgtgac tggccccact taatgggttt tcagaggtgg gagcaaagaa tgaagggagg
     5401 aagagagctc ccatcttttc cattaccgta tttacttaaa actggaaaaa actgaatcac
     5461 tgggatggta tttcatttat cttttgaaat gaagaataga tacattaatt taaatgattc
     5521 tctaaagact gtccttcagc atccctaaag cattgcaaaa ctataggcac agtttttatt
     5581 ttatctttct ggcacctggt ttgggctttg gcttagatta aaaagacgag ccttctcctg
     5641 cccctgctcc cttttattcc ctgccatctg ctaagctcga ctttttatct ctcaggccaa
     5701 atttgactct gtaagggact ttgcataacg tttcatcctc agaatcgcta atgatgggca
     5761 gaggactgtt acatgtgttg ggagggatta aagtatatga cctcagtgcc acttaaaaaa
     5821 taaatactgt ctcaactatc a
//
