LOCUS       NM_181725               5799 bp    mRNA    linear   PRI 08-DEC-2024
DEFINITION  Homo sapiens methyltransferase 2A, tRNA N3-cytidine (METTL2A),
            mRNA.
ACCESSION   NM_181725
VERSION     NM_181725.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5799)
  AUTHORS   Wang,S., Li,H., Liu,J., Zhang,Q., Xu,W., Xiang,J., Fang,L., Xu,P.
            and Li,Z.
  TITLE     Integrative analysis of m3C associated genes reveals METTL2A as a
            potential oncogene in breast Cancer
  JOURNAL   J Transl Med 20 (1), 476 (2022)
   PUBMED   36266694
  REMARK    GeneRIF: Integrative analysis of m3C associated genes reveals
            METTL2A as a potential oncogene in breast Cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5799)
  AUTHORS   Mao,X.L., Li,Z.H., Huang,M.H., Wang,J.T., Zhou,J.B., Li,Q.R.,
            Xu,H., Wang,X.J. and Zhou,X.L.
  TITLE     Mutually exclusive substrate selection strategy by human m3C RNA
            transferases METTL2A and METTL6
  JOURNAL   Nucleic Acids Res 49 (14), 8309-8323 (2021)
   PUBMED   34268557
  REMARK    GeneRIF: Mutually exclusive substrate selection strategy by human
            m3C RNA transferases METTL2A and METTL6.
REFERENCE   3  (bases 1 to 5799)
  AUTHORS   Lentini,J.M., Alsaif,H.S., Faqeih,E., Alkuraya,F.S. and Fu,D.
  TITLE     DALRD3 encodes a protein mutated in epileptic encephalopathy that
            targets arginine tRNAs for 3-methylcytosine modification
  JOURNAL   Nat Commun 11 (1), 2510 (2020)
   PUBMED   32427860
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5799)
  AUTHORS   Xu,L., Liu,X., Sheng,N., Oo,K.S., Liang,J., Chionh,Y.H., Xu,J.,
            Ye,F., Gao,Y.G., Dedon,P.C. and Fu,X.Y.
  TITLE     Three distinct 3-methylcytidine (m3C) methyltransferases modify
            tRNA and mRNA in mice and humans
  JOURNAL   J Biol Chem 292 (35), 14695-14703 (2017)
   PUBMED   28655767
  REMARK    GeneRIF: Data, including data from studies using mutant/knockout
            mice and cell lines from such mice, suggest that METTL2 and METTL6
            (but not METTL8) contribute to post-transcriptional methylation of
            cytidine (formation of 3-methylcytidine) in tRNA; METTL2 methylates
            tRNA(Thr) and tRNA(Arg); METTL6 interacts with seryl-tRNA
            synthetase and methylates tRNA(Ser); METTL8 catalyzes
            post-transcriptional methylation of cytidine in mRNA.
REFERENCE   5  (bases 1 to 5799)
  AUTHORS   Zody,M.C., Garber,M., Adams,D.J., Sharpe,T., Harrow,J.,
            Lupski,J.R., Nicholson,C., Searle,S.M., Wilming,L., Young,S.K.,
            Abouelleil,A., Allen,N.R., Bi,W., Bloom,T., Borowsky,M.L.,
            Bugalter,B.E., Butler,J., Chang,J.L., Chen,C.K., Cook,A., Corum,B.,
            Cuomo,C.A., de Jong,P.J., DeCaprio,D., Dewar,K., FitzGerald,M.,
            Gilbert,J., Gibson,R., Gnerre,S., Goldstein,S., Grafham,D.V.,
            Grocock,R., Hafez,N., Hagopian,D.S., Hart,E., Norman,C.H.,
            Humphray,S., Jaffe,D.B., Jones,M., Kamal,M., Khodiyar,V.K.,
            LaButti,K., Laird,G., Lehoczky,J., Liu,X., Lokyitsang,T.,
            Loveland,J., Lui,A., Macdonald,P., Major,J.E., Matthews,L.,
            Mauceli,E., McCarroll,S.A., Mihalev,A.H., Mudge,J., Nguyen,C.,
            Nicol,R., O'Leary,S.B., Osoegawa,K., Schwartz,D.C., Shaw-Smith,C.,
            Stankiewicz,P., Steward,C., Swarbreck,D., Venkataraman,V.,
            Whittaker,C.A., Yang,X., Zimmer,A.R., Bradley,A., Hubbard,T.,
            Birren,B.W., Rogers,J., Lander,E.S. and Nusbaum,C.
  TITLE     DNA sequence of human chromosome 17 and analysis of rearrangement
            in the human lineage
  JOURNAL   Nature 440 (7087), 1045-1049 (2006)
   PUBMED   16625196
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BP255803.1, BC006985.1, DA027807.1, AK022822.1 and AC008026.7.
            
            On Dec 2, 2019 this sequence version replaced NM_181725.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK022822.1, SRR14038193.2634843.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000311506.10/ ENSP00000309610.6
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-225               BP255803.1         13-237
            226-440             BC006985.1         440-654
            441-470             DA027807.1         441-470
            471-1349            BC006985.1         685-1563
            1350-1352           AK022822.1         1339-1341
            1353-5799           AC008026.7         100328-104774       c
FEATURES             Location/Qualifiers
     source          1..5799
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q23.2"
     gene            1..5799
                     /gene="METTL2A"
                     /gene_synonym="METTL2"
                     /note="methyltransferase 2A, tRNA N3-cytidine"
                     /db_xref="GeneID:339175"
                     /db_xref="HGNC:HGNC:25755"
                     /db_xref="MIM:618902"
     exon            1..116
                     /gene="METTL2A"
                     /gene_synonym="METTL2"
                     /inference="alignment:Splign:2.1.0"
     CDS             7..1143
                     /gene="METTL2A"
                     /gene_synonym="METTL2"
                     /note="methyltransferase-like protein 2A; tRNA
                     N(3)-methylcytidine methyltransferase METTL2A;
                     methyltransferase like 2A; methyltransferase 2A,
                     methylcytidine"
                     /codon_start=1
                     /product="tRNA N(3)-cytidine methyltransferase METTL2A"
                     /protein_id="NP_859076.3"
                     /db_xref="CCDS:CCDS45752.1"
                     /db_xref="GeneID:339175"
                     /db_xref="HGNC:HGNC:25755"
                     /db_xref="MIM:618902"
                     /translation="MAGSYPEGAPAVLADKRQQFGSRFLRDPARVFHHNAWDNVEWSE
                     EQAAAAERKVQENSIQRVCQEKQVDYEINAHKYWNDFYKIHENGFFKDRHWLFTEFPE
                     LAPSQNQNHLKDWFLENKSEVPECRNNEDGPGLIMEEQHKCSSKSLEHKTQTLPVEEN
                     VTQKISDLEICADEFPGSSATYRILEVGCGVGNTVFPILQTNNDPGLFVYCCDFSSTA
                     IELVQTNSEYDPSRCFAFVHDLCDEEKSYPVPKGSLDIIILIFVLSAIVPDKMQKAIN
                     RLSRLLKPGGMMLLRDYGRYDMAQLRFKKGQCLSGNFYVRGDGTRVYFFTQEELDTLF
                     TTAGLEKVQNLVDRRLQVNRGKQLTMYRVWIQCKYCKPLLSSTS"
     exon            117..208
                     /gene="METTL2A"
                     /gene_synonym="METTL2"
                     /inference="alignment:Splign:2.1.0"
     exon            209..564
                     /gene="METTL2A"
                     /gene_synonym="METTL2"
                     /inference="alignment:Splign:2.1.0"
     exon            565..614
                     /gene="METTL2A"
                     /gene_synonym="METTL2"
                     /inference="alignment:Splign:2.1.0"
     exon            615..675
                     /gene="METTL2A"
                     /gene_synonym="METTL2"
                     /inference="alignment:Splign:2.1.0"
     exon            676..815
                     /gene="METTL2A"
                     /gene_synonym="METTL2"
                     /inference="alignment:Splign:2.1.0"
     exon            816..922
                     /gene="METTL2A"
                     /gene_synonym="METTL2"
                     /inference="alignment:Splign:2.1.0"
     exon            923..988
                     /gene="METTL2A"
                     /gene_synonym="METTL2"
                     /inference="alignment:Splign:2.1.0"
     exon            989..5799
                     /gene="METTL2A"
                     /gene_synonym="METTL2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1332..1337
                     /regulatory_class="polyA_signal_sequence"
                     /gene="METTL2A"
                     /gene_synonym="METTL2"
                     /note="hexamer: AATAAA"
     polyA_site      1346
                     /gene="METTL2A"
                     /gene_synonym="METTL2"
                     /note="major polyA site"
     polyA_site      1593
                     /gene="METTL2A"
                     /gene_synonym="METTL2"
     regulatory      2129..2134
                     /regulatory_class="polyA_signal_sequence"
                     /gene="METTL2A"
                     /gene_synonym="METTL2"
                     /note="hexamer: AATAAA"
     regulatory      2135..2140
                     /regulatory_class="polyA_signal_sequence"
                     /gene="METTL2A"
                     /gene_synonym="METTL2"
                     /note="hexamer: AATAAA"
     polyA_site      2152
                     /gene="METTL2A"
                     /gene_synonym="METTL2"
     polyA_site      2163
                     /gene="METTL2A"
                     /gene_synonym="METTL2"
     regulatory      4481..4486
                     /regulatory_class="polyA_signal_sequence"
                     /gene="METTL2A"
                     /gene_synonym="METTL2"
                     /note="hexamer: AACAAA"
     regulatory      4499..4504
                     /regulatory_class="polyA_signal_sequence"
                     /gene="METTL2A"
                     /gene_synonym="METTL2"
                     /note="hexamer: AATAAA"
     polyA_site      4500
                     /gene="METTL2A"
                     /gene_synonym="METTL2"
     regulatory      4503..4508
                     /regulatory_class="polyA_signal_sequence"
                     /gene="METTL2A"
                     /gene_synonym="METTL2"
                     /note="hexamer: AATAAT"
     polyA_site      4517
                     /gene="METTL2A"
                     /gene_synonym="METTL2"
     polyA_site      4521
                     /gene="METTL2A"
                     /gene_synonym="METTL2"
     regulatory      5779..5784
                     /regulatory_class="polyA_signal_sequence"
                     /gene="METTL2A"
                     /gene_synonym="METTL2"
                     /note="hexamer: AATAAA"
     polyA_site      5799
                     /gene="METTL2A"
                     /gene_synonym="METTL2"
ORIGIN      
        1 ggtgtcatgg ccggctccta ccctgaaggt gcacctgcag tcctcgccga taagaggcag
       61 cagttcggaa gccggttcct gagagatccg gcgcgcgtct tccaccacaa tgcctgggac
      121 aatgtggagt ggtcggaaga gcaagccgcg gcggcggaga gaaaagtcca ggagaacagt
      181 atccagcggg tgtgccagga gaaacaagtt gattatgaga tcaatgccca caaatactgg
      241 aatgacttct acaaaatcca cgaaaatggg tttttcaagg atagacattg gctttttacc
      301 gaattccctg agctggcacc tagccaaaat caaaatcatt tgaaggactg gttcttggag
      361 aacaagagtg aagtacctga atgtagaaac aatgaggatg gacctggttt aataatggaa
      421 gaacagcaca agtgttcttc aaagagcctt gaacataaaa cacagacact tcctgtggag
      481 gagaatgtaa ctcagaaaat tagtgacctg gaaatttgtg ctgatgagtt tcctggatcc
      541 tcagccacct accgaatact ggaggttggc tgtggtgtgg gaaacacagt ctttccaatt
      601 ttacaaacga acaatgaccc aggactcttt gtttattgct gtgatttttc ttccacagct
      661 atagaactgg tccagacaaa ttcagaatat gatccttctc ggtgttttgc ctttgttcac
      721 gacctgtgtg atgaagagaa gagttaccca gtgcccaagg gcagtcttga tattatcatt
      781 ctcatatttg ttctttcagc aattgttcca gacaagatgc agaaggctat caacaggctg
      841 agcaggcttc tgaaacctgg cgggatgatg cttctgcgag attacggccg ctatgacatg
      901 gctcagcttc ggtttaaaaa aggtcagtgt ctatctggaa atttctacgt gagaggtgat
      961 ggaaccagag tttacttctt cacacaagag gaactggaca cgcttttcac cactgctgga
     1021 ctggaaaaag ttcagaacct ggtggatcgc cgactgcagg tgaaccgagg aaagcaactg
     1081 acaatgtacc gggtttggat tcagtgcaaa tactgcaagc cccttctgtc cagcaccagc
     1141 tgagaggcac ctgctgccaa cacgatgcaa gcccattgtg tttccgggct tttttaaaaa
     1201 aaaaattgta gcactgggcg tggtgcatgc ctgtaatccc agccactcag gaggctgagg
     1261 cggggaggat ccattgagcc cagcagtcca acctgggcaa aatagtgaga gaccctgtat
     1321 ctgaaagtaa taataaaaat aaaaaatata aatgaggtct cgttgatgtt ggacaattca
     1381 agaattcaga cttgaacctt aaacctagga aaagttactt tgtatcagga ttctaacaat
     1441 tatgcttcat atttgtgaag tcctttaaaa cataattttc tcaagttctt tctttgagat
     1501 ctcaatctgt cttagcattt tgtaactaat aactgaaatt ttattcaaag gaattgtaaa
     1561 ccttaaacca ccaatttatt tccatgtgaa aaagtgttac atatgacaag tgttttttga
     1621 ctgtaattgc gttaaatctt ttgagaatgt aaatgccggg ctaggcaatt gcagttaata
     1681 catacagagg ttagtgaagg gcttattaag ttgtagggga agcaagctgg gaagaatcag
     1741 atcagatatt ttcctgacaa aaaaaaatga ccctacagag agcatcaaaa tgtggtgttc
     1801 ttgttaagta attgatcgtg gtcttcgctt taatcttagt tccgccaggc acggtggctc
     1861 acacctgtaa tcccagcact ttgggaggcc aaggcgggcg gatcagctga ggtcaggagt
     1921 tggagaccag cgtggccaac atggtgaaac cccgtctcta ctaaagataa aaaaattagc
     1981 tgagtgtgtt ggtgggtgcc tgtaatccag ctacttggga gactgaggca ggagaattgc
     2041 ttgaacccag gaggcagagg ttgcagtgag ctgagattgt gccactgcac tccagcctgg
     2101 gcaacaaagc aagactctgt ctcaaaaaaa taaaaataaa aaaaaaatct tagttccatg
     2161 gaattttaag cattgttccc cctctaacct gtgtctaaag aattaaaaga atttggccgg
     2221 gtgcggcagc tcatgcctgt aatcccaaca ctttgggagg ctgaggcagg cggatcacga
     2281 ggtcaggaga tcgagaccat cctggccaac acggtgaaac ctcgtctcca ctaaaaatac
     2341 caaaaaaatt agctgggcat ggtggcacac acctgtagtc ccctgtagtc ccagctactc
     2401 gggaggctga ggcaggagaa tcgtttgaac ctgggaggtg gaggttgcag tgagccaaga
     2461 tcatgtcact gcattccagc ctgggcaaca gaggaagact ccgtctcaaa aaaaaaaaag
     2521 aaaagaattt tcactttttg caaaattatc ttttttaatg cacccttaag tgtttgacac
     2581 aaaaggaatt gagggaactt cttaatttta accacatcat cacttcactt ctgaatgtga
     2641 tcattatcag tgttagatgc cttttttttt tttttttttt tttttttttt aaggagacag
     2701 ggctctcact ttgttgccca ggctggagtt cagtggtgca atgatagctc attatcacct
     2761 caaacttctg gctcaagcca tccctccgcc tcagcctcct gagtagctag gactgcaggt
     2821 gggcaccatc atgcctagct aattgttaga tccttttttt ttatgttctg cattttttca
     2881 gttattttta catatttctc tcttgatcca tgcagttata ggatatatcc ttaattttag
     2941 aaagtaagga cttttcgacc aggcgtggcg gctcatgcct ataatcccag cactttggga
     3001 ggccgaggtg ggcagatcac ttgaggtcag gagttccagt ccagcctggc taacatagtg
     3061 aaaccccgtc tccactaaaa aataaaaaat cagcccggca tggctgtggg cgcctgtaat
     3121 cccagctact caggaggctg aagtagaatt gcttgaacct gggaggcaga ggttgcagtg
     3181 agccgagatg gtgccattgc actccagcct gggtgacaag agcgaaactg catctcagaa
     3241 acaaagatgc ctagctttca ccttgttgat gtttttctaa attttggaat aataaattta
     3301 tataggaata ctgcaagaat agtccatttt tttctgatgt cccaacatca ccaaatgtat
     3361 ccatcacaag taggaataca tggaaacatt aatgccagcc actaaccatt catgtttacc
     3421 cagtagtccc aataatagct ttgtcacaca aaaacatgat tatgttggct gggcacagtg
     3481 gctcctgtaa tcctagcatt ttgggagccc aaggcaggag ttcaagacca ggctgggtaa
     3541 tgtagcaaga ccctgacttt tttttttttt tttttttgag acagagcttc gctcttgttg
     3601 cccaggctgg agtacaatgg cacaatttca gctcaccgca acctctgcct cccaagttta
     3661 aatgattctc ctgcctcagc ctcccaagta gctggaatta caggcatgtg ccaccattcc
     3721 cggctaattt tttgtatttt tagtagagac agagtttctc catgttggtc aggctgatct
     3781 cgaactcccg acctcaggtg atctgcccgc cttggcctcc caaagtgctg ggattacagg
     3841 cgtgagccac cacgcccggc caagaccctg actttttaaa aaatgttaaa aataggccgg
     3901 gtgcggtggc tcacacctgt aatcccagca ctttgggagg ccaaggtggg cggatcattt
     3961 gaggtcggga gttcgagacc agcctggcca acttaatgaa accccatcac tgctaacaat
     4021 acagacctgg cacggtggct cacgcctgta atcccagcac tttgagagac caaggtaggt
     4081 ggatcacctg aggtcgggag ttggagacca gcctgaccaa catggagaaa ccccgtctct
     4141 actaaaaata caaaatagcc aggtgtgatg gcgcatgcct gtaaccccat ccacttggaa
     4201 ggctgaggca ggagaatcac ttgaatctgg gaggcggagg ttgcagtgag ccgagatggc
     4261 accattgcac tccagcctgg gcaacaagag tgaaactccg tctcaaaaaa aaaaaaaaaa
     4321 aaaattagct gggcatggtg gtgggcgcct gtaatcccag ctatttggga gggtggctct
     4381 agagaatcat ttgaacctgg gaggcggagg ttgcggtgag ccgagatcgc gccattgcac
     4441 tccagcctgg gcaacagtga gactccgtct caaaataaaa aacaaaaaaa atgtaaaaaa
     4501 taaataattg tattgaattt attagttgtg tctcagtctc cttcagtcta cgggttaggc
     4561 ttgcttttgc acctttgcat tttaaggtaa gaggctattg tgtaaactcg aattgggttt
     4621 gttggtgttt cttcatgatt tggttcaggc tctgcatttt gggcaggaat attcagaggt
     4681 aatgctgtgt tcctcccata ggatcgtatc aggcgacaca tgattatgaa tttccccatt
     4741 attggtgatg tcacttagtt gtgttgtctg ccgggttctg ccactgtaaa gttaataagt
     4801 attttgtagg gaagtgcttt gagactaaat atcctgttcc tcatcaaact tctaccccct
     4861 agtttcagta tctattgctg ctttttggct gaataactat gatagttgcc aaatagtggt
     4921 ttctgatgct tccattcctt ctacactgat tggttggtat tttgtacaga aaagctttct
     4981 catatctgat ttacttgtct caatatggac ccatgggtat ttgagtttct tgtggggtta
     5041 tttgttgttg tttttgagac aggctcaccc aggctggaat gcagcagcac gatcactgct
     5101 cactgcagcc tcgacctccc aggctcaggt gattctccca ccccagcctc ccaagtagat
     5161 gggactacag gcatgtgcca ccaagcctaa tttttgtatt ttttgtagag acagggtttc
     5221 atcatgtgtc ccaggctggt cttgaactcc tgagctcaag caatcctccc acctcggcct
     5281 cccaaagtgc tgggatgagc cactgcgact ggccccactt aatgggtttt cagaggtggg
     5341 agcaaagaat gaagggagga agagagctcc catcttttcc attactgtat ttacttaaaa
     5401 ctggaaaaaa ctgaatcact gggatggtat ttcatttatc ttttgaaatg aagaatagat
     5461 acattaattt aaatgattct ctaaagactg tccttcagca tccctaaagc atttcaacac
     5521 tataggcaca gtttttattt tatctttctg gcacctggtt tgggctttgg gcttagatta
     5581 aaaagacgag ccttctcctg cccctgctcc cttttattct ctgccatctg ctaagctcga
     5641 ctttttatct ctcaggccaa atttgactct gcaagggact ttgcataacg tttcatcctc
     5701 agaatcgcta atgatgggca gaggactgtt acatttgttg ggagggatta aagtatatga
     5761 cctcagtgcc acttaaaaaa taaatactgt ctcaactat
//
