LOCUS       NM_001127395            1326 bp    mRNA    linear   PRI 08-APR-2024
DEFINITION  Homo sapiens methyltransferase 21A, HSPA lysine (METTL21A),
            transcript variant 2, mRNA.
ACCESSION   NM_001127395
VERSION     NM_001127395.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1326)
  AUTHORS   Chen,S., Fragoza,R., Klei,L., Liu,Y., Wang,J., Roeder,K., Devlin,B.
            and Yu,H.
  TITLE     An interactome perturbation framework prioritizes damaging missense
            mutations for developmental disorders
  JOURNAL   Nat Genet 50 (7), 1032-1040 (2018)
   PUBMED   29892012
REFERENCE   4  (bases 1 to 1326)
  AUTHORS   Jakobsson,M.E., Moen,A., Bousset,L., Egge-Jacobsen,W.,
            Kernstock,S., Melki,R. and Falnes,P.O.
  TITLE     Identification and characterization of a novel human
            methyltransferase modulating Hsp70 protein function through lysine
            methylation
  JOURNAL   J Biol Chem 288 (39), 27752-27763 (2013)
   PUBMED   23921388
  REMARK    GeneRIF: methyltransferase METTL21A is the enzyme responsible for
            trimethylation of a conserved lysine residue found in several human
            Hsp70 (HSPA) proteins
REFERENCE   5  (bases 1 to 1326)
  AUTHORS   Cloutier,P., Lavallee-Adam,M., Faubert,D., Blanchette,M. and
            Coulombe,B.
  TITLE     A newly uncovered group of distantly related lysine
            methyltransferases preferentially interact with molecular
            chaperones to regulate their activity
  JOURNAL   PLoS Genet 9 (1), e1003210 (2013)
   PUBMED   23349634
  REMARK    GeneRIF: METTL21A trimethylates Lys-561 in Hsp70 and corresponding
            residues in other isoforms (Hsc70, BiP)
REFERENCE   6  (bases 1 to 1326)
  AUTHORS   Kernstock,S., Davydova,E., Jakobsson,M., Moen,A., Pettersen,S.,
            Maelandsmo,G.M., Egge-Jacobsen,W. and Falnes,P.O.
  TITLE     Lysine methylation of VCP by a member of a novel human protein
            methyltransferase family
  JOURNAL   Nat Commun 3, 1038 (2012)
   PUBMED   22948820
REFERENCE   7  (bases 1 to 1326)
  AUTHORS   Yu,H., Tardivo,L., Tam,S., Weiner,E., Gebreab,F., Fan,C.,
            Svrzikapa,N., Hirozane-Kishikawa,T., Rietman,E., Yang,X.,
            Sahalie,J., Salehi-Ashtiani,K., Hao,T., Cusick,M.E., Hill,D.E.,
            Roth,F.P., Braun,P. and Vidal,M.
  TITLE     Next-generation sequencing to generate interactome datasets
  JOURNAL   Nat Methods 8 (6), 478-480 (2011)
   PUBMED   21516116
REFERENCE   8  (bases 1 to 1326)
  AUTHORS   Harrington,J.J., Sherf,B., Rundlett,S., Jackson,P.D., Perry,R.,
            Cain,S., Leventhal,C., Thornton,M., Ramachandran,R.,
            Whittington,J., Lerner,L., Costanzo,D., McElligott,K., Boozer,S.,
            Mays,R., Smith,E., Veloso,N., Klika,A., Hess,J., Cothren,K., Lo,K.,
            Offenbacher,J., Danzig,J. and Ducar,M.
  TITLE     Creation of genome-wide protein expression libraries using random
            activation of gene expression
  JOURNAL   Nat Biotechnol 19 (5), 440-445 (2001)
   PUBMED   11329013
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC079767.7.
            
            On Jun 16, 2021 this sequence version replaced NM_001127395.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038193.3912408.1, BQ943496.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000411432.6/ ENSP00000415115.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-333               AC079767.7         35016-35348         c
            334-509             AC079767.7         34183-34358         c
            510-621             AC079767.7         31760-31871         c
            622-1326            AC079767.7         22693-23397         c
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q33.3"
     gene            1..1326
                     /gene="METTL21A"
                     /gene_synonym="FAM119A; HCA557b; HSPA-KMT"
                     /note="methyltransferase 21A, HSPA lysine"
                     /db_xref="GeneID:151194"
                     /db_xref="HGNC:HGNC:30476"
                     /db_xref="MIM:615257"
     exon            1..333
                     /gene="METTL21A"
                     /gene_synonym="FAM119A; HCA557b; HSPA-KMT"
                     /inference="alignment:Splign:2.1.0"
     exon            334..509
                     /gene="METTL21A"
                     /gene_synonym="FAM119A; HCA557b; HSPA-KMT"
                     /inference="alignment:Splign:2.1.0"
     CDS             363..1019
                     /gene="METTL21A"
                     /gene_synonym="FAM119A; HCA557b; HSPA-KMT"
                     /note="isoform 1 is encoded by transcript variant 2;
                     family with sequence similarity 119, member A; protein
                     N-lysine methyltransferase METTL21A; heat shock protein
                     70kDa lysine (K) methyltransferase; HSPA lysine
                     methyltransferase; hepatocellular carcinoma-associated
                     antigen 557b; methyltransferase-like protein 21A;
                     methyltransferase like 21A"
                     /codon_start=1
                     /product="protein N-lysine methyltransferase METTL21A
                     isoform 1"
                     /protein_id="NP_001120867.1"
                     /db_xref="CCDS:CCDS2376.1"
                     /db_xref="GeneID:151194"
                     /db_xref="HGNC:HGNC:30476"
                     /db_xref="MIM:615257"
                     /translation="MALVPYEETTEFGLQKFHKPLATFSFANHTIQIRQDWRHLGVAA
                     VVWDAAIVLSTYLEMGAVELRGRSAVELGAGTGLVGIVAALLGAHVTITDRKVALEFL
                     KSNVQANLPPHIQTKTVVKELTWGQNLGSFSPGEFDLILGADIIYLEETFTDLLQTLE
                     HLCSNHSVILLACRIRYERDNNFLAMLERQFTVRKVHYDPEKDVHIYEAQKRNQKEDL
                     "
     exon            510..621
                     /gene="METTL21A"
                     /gene_synonym="FAM119A; HCA557b; HSPA-KMT"
                     /inference="alignment:Splign:2.1.0"
     exon            622..1326
                     /gene="METTL21A"
                     /gene_synonym="FAM119A; HCA557b; HSPA-KMT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1236..1241
                     /regulatory_class="polyA_signal_sequence"
                     /gene="METTL21A"
                     /gene_synonym="FAM119A; HCA557b; HSPA-KMT"
                     /note="hexamer: ATTAAA"
     polyA_site      1254
                     /gene="METTL21A"
                     /gene_synonym="FAM119A; HCA557b; HSPA-KMT"
                     /note="major polyA site"
     regulatory      1299..1304
                     /regulatory_class="polyA_signal_sequence"
                     /gene="METTL21A"
                     /gene_synonym="FAM119A; HCA557b; HSPA-KMT"
                     /note="hexamer: AATAAA"
     polyA_site      1326
                     /gene="METTL21A"
                     /gene_synonym="FAM119A; HCA557b; HSPA-KMT"
                     /note="major polyA site"
ORIGIN      
        1 ggaaatgcgt ggctccgggc cggggccgcc cgcccccgcg tcgcgggctc tttaaggccg
       61 gcggttttcg cagccagccc ggggcgggga aagcggagcg cgcgctccac gcgggaccgc
      121 ctcccgggcc gtaagtaccg gcgtggcggc gcctcagccc ggcctgggcg agccctgggt
      181 gctccgccgg gcagctcacg gcgccccgta tggcctgggg atcctaagag gccctgtgac
      241 ccccctcgcc tggtctccct ctcacccctg gagggttgcc gcagctccgg ggcccccggg
      301 caggaagggc gcactggtcg tcccgggaga ggggtctgag cagagggcgg ggtgcaggcg
      361 gaatggccct cgtgccctat gaggagacca cggaatttgg gttgcagaaa ttccacaagc
      421 ctcttgcaac tttttccttt gcaaaccaca cgatccagat ccggcaggac tggagacacc
      481 tgggagtcgc agcggtggtt tgggatgcgg ccatcgttct ttccacatac ctggagatgg
      541 gagctgtgga gctcaggggc cgctctgccg tggagctggg tgctggcacg gggctggtgg
      601 gcatagtggc tgccctgctg ggtgctcatg tgactatcac ggatcgaaaa gtagcattag
      661 aatttcttaa atcaaacgtt caagccaact tacctcctca tatccaaact aaaactgttg
      721 ttaaggagct gacttgggga caaaatttgg ggagtttttc tcctggagaa tttgacctga
      781 tacttggtgc tgatatcata tatttagaag aaacattcac agatcttctt cagacactgg
      841 aacatctctg tagcaatcac tctgtgattc ttttagcatg ccgaattcgc tatgaacggg
      901 ataacaactt cttagcaatg ctggagaggc aatttactgt gagaaaggtt cactacgatc
      961 ctgaaaaaga tgtacatatt tacgaagcac agaagagaaa ccagaaggag gacttataat
     1021 tggctataat ttataagaat gttgtcattg agtgtgtcac ttaaggtctt agactgcaaa
     1081 tctaaccata tttaatgaaa tgtcttactg tacaaaaagt ctaagccaaa ggttctcagg
     1141 ggagaaagca catgtgcagt tttaaaacaa agcagtgctt tgtcccattg ctgtgatttt
     1201 tagtcagact ttactcagtc tgaaatgcaa ttaacattaa aggattaagt gtgagatttc
     1261 gatttatgct atttgtgtat cccatactcc tcccttttaa taaacagttt ccactgatga
     1321 tatgaa
//
