LOCUS       NM_001113528            4208 bp    mRNA    linear   PRI 08-DEC-2024
DEFINITION  Homo sapiens methyltransferase 15, mitochondrial 12S rRNA
            N4-cytidine (METTL15), transcript variant 1, mRNA.
ACCESSION   NM_001113528
VERSION     NM_001113528.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Mutti,C.D., Van Haute,L. and Minczuk,M.
  TITLE     The catalytic activity of methyltransferase METTL15 is dispensable
            for its role in mitochondrial ribosome biogenesis
  JOURNAL   RNA Biol 21 (1), 23-30 (2024)
   PUBMED   38913872
  REMARK    GeneRIF: The catalytic activity of methyltransferase METTL15 is
            dispensable for its role in mitochondrial ribosome biogenesis.
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Guo,F., Luo,Y., Ye,G. and Tang,W.
  TITLE     Circular RNA METTL15/miR-374a-5p/ESCO2 axis induces colorectal
            cancer development
  JOURNAL   Acta Biochim Pol 70 (3), 541-549 (2023)
   PUBMED   37715994
  REMARK    GeneRIF: Circular RNA METTL15/miR-374a-5p/ESCO2 axis induces
            colorectal cancer development.
REFERENCE   3  (bases 1 to 4208)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   4  (bases 1 to 4208)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 4208)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   6  (bases 1 to 4208)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   7  (bases 1 to 4208)
  AUTHORS   Van Haute,L., Hendrick,A.G., D'Souza,A.R., Powell,C.A.,
            Rebelo-Guiomar,P., Harbour,M.E., Ding,S., Fearnley,I.M., Andrews,B.
            and Minczuk,M.
  TITLE     METTL15 introduces N4-methylcytidine into human mitochondrial 12S
            rRNA and is required for mitoribosome biogenesis
  JOURNAL   Nucleic Acids Res 47 (19), 10267-10281 (2019)
   PUBMED   31665743
REFERENCE   8  (bases 1 to 4208)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   9  (bases 1 to 4208)
  AUTHORS   Antonicka,H., Choquet,K., Lin,Z.Y., Gingras,A.C., Kleinman,C.L. and
            Shoubridge,E.A.
  TITLE     A pseudouridine synthase module is essential for mitochondrial
            protein synthesis and cell viability
  JOURNAL   EMBO Rep 18 (1), 28-38 (2017)
   PUBMED   27974379
REFERENCE   10 (bases 1 to 4208)
  AUTHORS   Comuzzie,A.G., Cole,S.A., Laston,S.L., Voruganti,V.S., Haack,K.,
            Gibbs,R.A. and Butte,N.F.
  TITLE     Novel genetic loci identified for the pathophysiology of childhood
            obesity in the Hispanic population
  JOURNAL   PLoS One 7 (12), e51954 (2012)
   PUBMED   23251661
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DB458698.1, AL832668.1, AC087376.5, AK091298.1 and DB327106.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001113528.1.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.230769.1,
                                           SRR1803614.150672.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            MANE Ensembl match                 :: ENST00000407364.8/
                                                  ENSP00000384369.3
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-215               DB458698.1         141-355
            216-1503            AL832668.1         6-1293
            1504-2286           AC087376.5         17430-18212
            2287-3654           AK091298.1         2147-3514
            3655-4097           AK091298.1         3517-3959
            4098-4208           DB327106.1         1-111               c
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p14.1"
     gene            1..4208
                     /gene="METTL15"
                     /gene_synonym="METT5D1"
                     /note="methyltransferase 15, mitochondrial 12S rRNA
                     N4-cytidine"
                     /db_xref="GeneID:196074"
                     /db_xref="HGNC:HGNC:26606"
                     /db_xref="MIM:618711"
     exon            1..65
                     /gene="METTL15"
                     /gene_synonym="METT5D1"
                     /inference="alignment:Splign:2.1.0"
     exon            66..301
                     /gene="METTL15"
                     /gene_synonym="METT5D1"
                     /inference="alignment:Splign:2.1.0"
     exon            302..588
                     /gene="METTL15"
                     /gene_synonym="METT5D1"
                     /inference="alignment:Splign:2.1.0"
     CDS             319..1542
                     /gene="METTL15"
                     /gene_synonym="METT5D1"
                     /EC_number="2.1.1.-"
                     /note="isoform 1 is encoded by transcript variant 1;
                     methyltransferase 5 domain containing 1; putative
                     S-adenosyl-L-methionine-dependent methyltransferase
                     METT5D1; probable S-adenosyl-L-methionine-dependent
                     methyltransferase METT5D1; probable methyltransferase-like
                     protein 15; methyltransferase 5 domain-containing protein
                     1; 12S rRNA m4C methyltransferase; 12S rRNA
                     N4-methylcytidine (m4C) methyltransferase;
                     methyltransferase like 15; 12S rRNA N(4)-cytidine
                     methyltransferase METTL15"
                     /codon_start=1
                     /product="12S rRNA N(4)-cytidine methyltransferase METTL15
                     isoform 1"
                     /protein_id="NP_001107000.1"
                     /db_xref="CCDS:CCDS44559.1"
                     /db_xref="GeneID:196074"
                     /db_xref="HGNC:HGNC:26606"
                     /db_xref="MIM:618711"
                     /translation="MLRYPYFCRMYKECLSCWLESGIPNLGVWPNRIHTTAEKYREYE
                     AREQTDQTQAQELHRSQDRDFETMAKLHIPVMVDEVVHCLSPQKGQIFLDMTFGSGGH
                     TKAILQKESDIVLYALDRDPTAYALAEHLSELYPKQIRAMLGQFSQAEALLMKAGVQP
                     GTFDGVLMDLGCSSMQLDTPERGFSLRKDGPLDMRMDGGRYPDMPTAADVVNALDQQA
                     LASILRTYGEEKHAKKIASAIVQARSIYPITRTQQLASIVAGAFPPSAIYTRKDLLQR
                     STHIATKTFQALRIFVNNELNELYTGLKTAQKFLRPGGRLVALSFHSLEDRIVKRFLL
                     GISMTERFNLSVRQQVMKTSQLGSDHENTEEVSMRRAPLMWELIHKKVLSPQDQDVQD
                     NPRGRSAKLRAAIKL"
     exon            589..725
                     /gene="METTL15"
                     /gene_synonym="METT5D1"
                     /inference="alignment:Splign:2.1.0"
     exon            726..917
                     /gene="METTL15"
                     /gene_synonym="METT5D1"
                     /inference="alignment:Splign:2.1.0"
     exon            918..1096
                     /gene="METTL15"
                     /gene_synonym="METT5D1"
                     /inference="alignment:Splign:2.1.0"
     exon            1097..4208
                     /gene="METTL15"
                     /gene_synonym="METT5D1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4188..4193
                     /regulatory_class="polyA_signal_sequence"
                     /gene="METTL15"
                     /gene_synonym="METT5D1"
                     /note="hexamer: AATAAA"
     polyA_site      4208
                     /gene="METTL15"
                     /gene_synonym="METT5D1"
                     /note="major polyA site"
ORIGIN      
        1 agccacttga gatcaccagg cctcctgtag accggcgggt gcagggcccg gtcccagagt
       61 taagggaaag tcgtttggaa accccaggcc aacaggaccc tttggcagct gaggctggaa
      121 acagcggaac caaaggcaga cggtcctcag ttgcttgggc ggacgtggac ccaatctgtc
      181 cgtccttaca agtttccccc aggggcagga cctagacgcg cggcgcattc aggttctcct
      241 ggaccgtagt ggtagtcgct ggcccgagtt agaggccagc tgagaagtct tcgtgcttca
      301 gatttgttta cctacaaaat gcttcggtat ccatattttt gtagaatgta taaagaatgc
      361 ctttcatgtt ggttggaatc tggcatacct aatttaggtg tctggccaaa cagaatacat
      421 actacagcag aaaaatatag agaatatgaa gcccgggagc aaacagatca aactcaagcc
      481 caggagttac acagatctca agatagagat tttgaaacta tggctaaatt acatattcca
      541 gtaatggtgg atgaagttgt tcattgtttg tcaccacaaa aaggacagat ttttctagat
      601 atgacatttg gttcgggagg gcacacaaaa gccattctgc agaaggagtc agatattgtt
      661 ctctatgcct tggacagaga cccaacagct tatgcattag ctgaacatct ttcagagttg
      721 tatcctaaac aaatccgagc tatgctgggc cagttcagcc aggcagaagc cttattaatg
      781 aaagctggag tgcagccagg aacttttgat ggagttctta tggatcttgg gtgttcctcc
      841 atgcaacttg atactcctga aagaggtttt tcccttcgga aagatggccc tttggacatg
      901 agaatggatg gtggcaggta ccctgacatg cccactgctg ctgatgttgt gaatgcttta
      961 gatcaacagg cacttgcatc tatcctaaga acatacgggg aggagaagca tgccaagaaa
     1021 atcgcttcag caattgttca ggcacgcagc atctacccca tcaccagaac ccagcagctt
     1081 gccagcatcg ttgcaggagc atttcctccc tctgctattt atacacggaa agacttacta
     1141 cagcgatcta cccatattgc caccaagact ttccaggctc ttcgcatatt tgtgaacaat
     1201 gagctcaatg aactctacac gggactgaag acagctcaga agtttctgag acctggtggt
     1261 cgtcttgttg ccctctcctt ccattcacta gaggatcgca tcgtcaaaag atttttgctt
     1321 ggaataagca tgacagaaag atttaaccta agtgttagac aacaagtgat gaaaacatct
     1381 caattgggtt cagatcacga aaacacggaa gaagtctcta tgagaagagc tcctttaatg
     1441 tgggaattga tacacaagaa ggtacttagt ccacaagatc aggatgtaca agataacccc
     1501 agagggcgct cagccaagct tagagcagct atcaaattat aagttatcat catcttattc
     1561 ttcaaatttt tttctcacaa tttctctaat ctttactcat gttatgtccc tgaatgtctt
     1621 ggtataggtt taagtgtggg acagtctgaa aattgatagc atttagcatt tcttttttct
     1681 caaaaagaaa ctgtaggaaa tacatgacag agaaagttac actcagggag cagcagcacc
     1741 tccagactgg aaaaatatgt taatctttgc atcatattgg actcttgaag gcaatccttc
     1801 ctctggccag gaaatttttt aaaaaataat actgtgttgt gtttatctaa atacgtaaac
     1861 tcaagctacc aaagagaaag atgttgtaat catatctgca tgtcctaaat tttgaattta
     1921 gatattccaa tttgcatcag tctttctgtg gctcaaataa tgatgattat ggaatgaatt
     1981 ttaatgtccc tacttgtgaa taattagctt tctcaaatgt aggcttttta caaattttaa
     2041 attttaaaat attagtttaa atgtgtgtta tactgataaa atttcatctt tcaaattata
     2101 gtctattatt ttaaagggat ttttcagtat gatatgggcc attttgttca tctatcgcaa
     2161 agtaaaaatg taaaatcctt acagagaatt gtttcacaaa acttatattt catgtcaatt
     2221 gtatttattt taataatagc tcacaatgcc tttagtaagt aataaagtct cttattagaa
     2281 tcttgtattt tttaattgag ctaatcaaaa taattcagcc aagtctattt gaaatagaaa
     2341 actgtctatt taatatagta aaatcaatgc tcccttaatg ttgttacaaa gatatggtaa
     2401 ctgtaatatg ggtaaaagtt tattcaagaa aagagtactt ggtagaagat tctttaacaa
     2461 attgaggaga ttgattcata attcacatgt caatttttta tagtaatatg tacttctaat
     2521 ttattattta tacttaaatt gtgacctgaa agatgagttg gagataatta gtcaaagggc
     2581 acatggagta tggaaggggg catgttgatt aaccatacat agaaataaat attctcttag
     2641 ttatccttga aatcatattt atactattaa tttgctcagt gcactttttt caaatacaga
     2701 aaaaagattc tccattatag ttacaaattt taggttggtg aatccaagtg aatgaaatat
     2761 cagcataact atgtgggcaa aatagataga attaaatgca tgaattttcc tcagacttat
     2821 tttatctgcc tctgtaatat ttaactttag tacccaggct tgttcactac ttctgcttta
     2881 caggctttat ttaaatctga acaatcctac agggaaatca gaattaggaa aaaaatctgg
     2941 agaacaatat ggttgaaata gaaacaagag aatgcaccta ggttaattcc ctgaatccta
     3001 cttgaacatt gtataaattt ctctttgcat ataatacata tttgtgaatg agacatattc
     3061 ccaaaaaatt cttatctctg tatgtgattg gaaaagaaaa gatcacattt gtatattcaa
     3121 caatctttca cctatttcat aagtcatttt ttcaccctgt atagtatggg aattattttt
     3181 tatgttaaat agaaactgaa tgtactgggt tgaatggtgt cctctccaaa attcatgtac
     3241 ttcctggagc ctcagtatgt gaccttattt ggaaatactg tggttgtggt tgtaagtagc
     3301 taagatgagg tcatactgga gcagggcagg cccttaatcc aatatgactg gtgttcctta
     3361 taagagaaga tagggcgggc atggtggctc acgcctgtaa tcccagcact gtgggaggcc
     3421 aagccaggca aatcgcttga ggctgaggag ttcaagacca gcctggccaa catggcgaaa
     3481 acccatctct actaaaaata aaattagcca tgcgtggtgc ttgtaatgtc agctacccag
     3541 gagtctgagg cacaagaatc actcgaacct gggaggtgga ggttgcgaga tcacaccact
     3601 gcactccagt gtgagcaaca gaacgagact ctgtctcaaa aaaaaaaaaa aaaaggaaga
     3661 aagagaagat agagacacag gggagaatgc cacatgaagc tggaggcaga gattggagtg
     3721 atacattgtg ggaaccctca gaagatagga aaaaagcatg gaatagattc tccctcagag
     3781 ttccagtagt tgccaaccct gctaacatgg ttttgcacat ctagcatcca gaacagtgag
     3841 aatgtatttc tgctgtttta aggtacccag ttcatggtaa tttgttacag caatcctagg
     3901 aagctaatac tctgacagaa aaaaatcttg gataatatgc atgagaaaaa tccttcaata
     3961 aacacaaaat tggtttcaaa atgttttcat gtaaaaataa attaactttt ctgtaattag
     4021 attgtgtttt atagtaattc ttgcctgctt cttagtcttt gttgtaggat gcataataaa
     4081 atacatgaat gagttttaaa tggttagctt tggagaatgg ttttgggaag ttcctacagt
     4141 acacagaaat aaaagctaat gttactatga atgcgcatta cttttctaat aaaatgtttc
     4201 tgaatctc
//
