LOCUS       NM_006838               3400 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens methionyl aminopeptidase 2 (METAP2), transcript
            variant 1, mRNA.
ACCESSION   NM_006838
VERSION     NM_006838.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Santhosh Kumar,S., Naseri,N.N., Pather,S.R., Hallacli,E.,
            Ndayisaba,A., Buenaventura,C., Acosta,K., Roof,J., Fazelinia,H.,
            Spruce,L.A., Luk,K., Khurana,V., Rhoades,E. and Shalem,O.
  TITLE     Sequential CRISPR screening reveals partial NatB inhibition as a
            strategy to mitigate alpha-synuclein levels in human neurons
  JOURNAL   Sci Adv 10 (6), eadj4767 (2024)
   PUBMED   38335281
  REMARK    GeneRIF: Sequential CRISPR screening reveals partial NatB
            inhibition as a strategy to mitigate alpha-synuclein levels in
            human neurons.
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 3400)
  AUTHORS   Xie,J., Rice,M.A., Chen,Z., Cheng,Y., Hsu,E.C., Chen,M., Song,G.,
            Cui,L., Zhou,K., Castillo,J.B., Zhang,C.A., Shen,B., Chin,F.T.,
            Kunder,C.A., Brooks,J.D., Stoyanova,T. and Rao,J.
  TITLE     In Vivo Imaging of Methionine Aminopeptidase II for Prostate Cancer
            Risk Stratification
  JOURNAL   Cancer Res 81 (9), 2510-2521 (2021)
   PUBMED   33637565
  REMARK    GeneRIF: In Vivo Imaging of Methionine Aminopeptidase II for
            Prostate Cancer Risk Stratification.
REFERENCE   4  (bases 1 to 3400)
  AUTHORS   He,W.P., Guo,Y.Y., Yang,G.P., Lai,H.L., Sun,T.T., Zhang,Z.W.,
            Ouyang,L.L., Zheng,Y., Tian,L.M., Li,X.H., You,Z.S., Xie,D. and
            Yang,G.F.
  TITLE     CHD1L promotes EOC cell invasiveness and metastasis via the
            regulation of METAP2
  JOURNAL   Int J Med Sci 17 (15), 2387-2395 (2020)
   PUBMED   32922205
  REMARK    GeneRIF: CHD1L promotes EOC cell invasiveness and metastasis via
            the regulation of METAP2.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3400)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   6  (bases 1 to 3400)
  AUTHORS   Joharapurkar,A.A., Dhanesha,N.A. and Jain,M.R.
  TITLE     Inhibition of the methionine aminopeptidase 2 enzyme for the
            treatment of obesity
  JOURNAL   Diabetes Metab Syndr Obes 7, 73-84 (2014)
   PUBMED   24611021
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 3400)
  AUTHORS   Arfin,S.M., Kendall,R.L., Hall,L., Weaver,L.H., Stewart,A.E.,
            Matthews,B.W. and Bradshaw,R.A.
  TITLE     Eukaryotic methionyl aminopeptidases: two classes of
            cobalt-dependent enzymes
  JOURNAL   Proc Natl Acad Sci U S A 92 (17), 7714-7718 (1995)
   PUBMED   7644482
REFERENCE   8  (bases 1 to 3400)
  AUTHORS   Prigmore,E., Ahmed,S., Best,A., Kozma,R., Manser,E., Segal,A.W. and
            Lim,L.
  TITLE     A 68-kDa kinase and NADPH oxidase component p67phox are targets for
            Cdc42Hs and Rac1 in neutrophils
  JOURNAL   J Biol Chem 270 (18), 10717-10722 (1995)
   PUBMED   7738010
REFERENCE   9  (bases 1 to 3400)
  AUTHORS   Li,X. and Chang,Y.H.
  TITLE     Molecular cloning of a human complementary DNA encoding an
            initiation factor 2-associated protein (p67)
  JOURNAL   Biochim Biophys Acta 1260 (3), 333-336 (1995)
   PUBMED   7873610
REFERENCE   10 (bases 1 to 3400)
  AUTHORS   Ray,M.K., Chakraborty,A., Datta,B., Chattopadhyay,A., Saha,D.,
            Bose,A., Kinzy,T.G., Wu,S., Hileman,R.E., Merrick,W.C. et al.
  TITLE     Characteristics of the eukaryotic initiation factor 2 associated
            67-kDa polypeptide
  JOURNAL   Biochemistry 32 (19), 5151-5159 (1993)
   PUBMED   8098621
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from U13261.1 and AC018475.32.
            
            On Nov 22, 2018 this sequence version replaced NM_006838.3.
            
            Summary: The protein encoded by this gene is a member of the
            methionyl aminopeptidase family. The encoded protein functions both
            by protecting the alpha subunit of eukaryotic initiation factor 2
            from inhibitory phosphorylation and by removing the amino-terminal
            methionine residue from nascent proteins. Increased expression of
            this gene is associated with various forms of cancer, and the
            anti-cancer drugs fumagillin and ovalicin inhibit the protein by
            irreversibly binding to its active site. Inhibitors of this gene
            have also been shown to be effective for the treatment of obesity.
            A pseudogene of this gene is located on chromosome 2. Several
            transcript variants encoding different isoforms have been found for
            this gene. [provided by RefSeq, Nov 2015].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.27760.1,
                                           SRR1660805.235421.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000323666.10/ ENSP00000325312.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1596              U13261.1           7-1602
            1597-3400           AC018475.32        49451-51254         c
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q22"
     gene            1..3400
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /note="methionyl aminopeptidase 2"
                     /db_xref="GeneID:10988"
                     /db_xref="HGNC:HGNC:16672"
                     /db_xref="MIM:601870"
     exon            1..179
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     CDS             29..1465
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /EC_number="3.4.11.18"
                     /note="isoform 1 is encoded by transcript variant 1;
                     initiation factor 2-associated 67 kDa glycoprotein;
                     peptidase M 2; eIF-2-associated p67 homolog; testicular
                     tissue protein Li 17; methionine aminopeptidase 2"
                     /codon_start=1
                     /product="methionine aminopeptidase 2 isoform 1"
                     /protein_id="NP_006829.1"
                     /db_xref="CCDS:CCDS9052.1"
                     /db_xref="GeneID:10988"
                     /db_xref="HGNC:HGNC:16672"
                     /db_xref="MIM:601870"
                     /translation="MAGVEEVAASGSHLNGDLDPDDREEGAASTAEEAAKKKRRKKKK
                     SKGPSAAGEQEPDKESGASVDEVARQLERSALEDKERDEDDEDGDGDGDGATGKKKKK
                     KKKKRGPKVQTDPPSVPICDLYPNGVFPKGQECEYPPTQDGRTAAWRTTSEEKKALDQ
                     ASEEIWNDFREAAEAHRQVRKYVMSWIKPGMTMIEICEKLEDCSRKLIKENGLNAGLA
                     FPTGCSLNNCAAHYTPNAGDTTVLQYDDICKIDFGTHISGRIIDCAFTVTFNPKYDTL
                     LKAVKDATNTGIKCAGIDVRLCDVGEAIQEVMESYEVEIDGKTYQVKPIRNLNGHSIG
                     QYRIHAGKTVPIVKGGEATRMEEGEVYAIETFGSTGKGVVHDDMECSHYMKNFDVGHV
                     PIRLPRTKHLLNVINENFGTLAFCRRWLDRLGESKYLMALKNLCDLGIVDPYPPLCDI
                     KGSYTAQFEHTILLRPTCKEVVSRGDDY"
     exon            180..287
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            288..353
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            354..456
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            457..618
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            619..800
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            801..895
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            896..992
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            993..1096
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1097..1212
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1213..3400
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1882..1887
                     /regulatory_class="polyA_signal_sequence"
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /note="hexamer: AATAAA"
     polyA_site      1904
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /note="major polyA site"
     regulatory      3378..3383
                     /regulatory_class="polyA_signal_sequence"
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /note="hexamer: AGTAAA"
     polyA_site      3400
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
ORIGIN      
        1 ctcattccct cgcgctctct cgggcaacat ggcgggtgtg gaggaggtag cggcctccgg
       61 gagccacctg aatggcgacc tggatccaga cgacagggaa gaaggagctg cctctacggc
      121 tgaggaagca gccaagaaaa aaagacgaaa gaagaagaag agcaaagggc cttctgcagc
      181 aggggaacag gaacctgata aagaatcagg agcctcagtg gatgaagtag caagacagtt
      241 ggaaagatca gcattggaag ataaagaaag agatgaagat gatgaagatg gagatggcga
      301 tggagatgga gcaactggaa agaagaagaa aaagaagaag aagaagagag gaccaaaagt
      361 tcaaacagac cctccctcag ttccaatatg tgacctgtat cctaatggtg tatttcccaa
      421 aggacaagaa tgcgaatacc cacccacaca agatgggcga acagctgctt ggagaactac
      481 aagtgaagaa aagaaagcat tagatcaggc aagtgaagag atttggaatg attttcgaga
      541 agctgcagaa gcacatcgac aagttagaaa atacgtaatg agctggatca agcctgggat
      601 gacaatgata gaaatctgtg aaaagttgga agactgttca cgcaagttaa taaaagagaa
      661 tggattaaat gcaggcctgg catttcctac tggatgttct ctcaataatt gtgctgccca
      721 ttatactccc aatgccggtg acacaacagt attacagtat gatgacatct gtaaaataga
      781 ctttggaaca catataagtg gtaggattat tgactgtgct tttactgtca cttttaatcc
      841 caaatatgat acgttattaa aagctgtaaa agatgctact aacactggaa taaagtgtgc
      901 tggaattgat gttcgtctgt gtgatgttgg tgaggccatc caagaagtta tggagtccta
      961 tgaagttgaa atagatggga agacatatca agtgaaacca atccgtaatc taaatggaca
     1021 ttcaattggg caatatagaa tacatgctgg aaaaacagtg ccgattgtga aaggagggga
     1081 ggcaacaaga atggaggaag gagaagtata tgcaattgaa acctttggta gtacaggaaa
     1141 aggtgttgtt catgatgata tggaatgttc acattacatg aaaaattttg atgttggaca
     1201 tgtgccaata aggcttccaa gaacaaaaca cttgttaaat gtcatcaatg aaaactttgg
     1261 aacccttgcc ttctgccgca gatggctgga tcgcttggga gaaagtaaat acttgatggc
     1321 tctgaagaat ctgtgtgact tgggcattgt agatccatat ccaccattat gtgacattaa
     1381 aggatcatat acagcgcaat ttgaacatac catcctgttg cgtccaacat gtaaagaagt
     1441 tgtcagcaga ggagatgact attaaactta gtccaaagcc acctcaacac ctttattttc
     1501 tgagctttgt tggaaaacat gataccagaa ttaatttgcc acatgttgtc tgttttaaca
     1561 gtggacccat gtaatacttt tatccatgtt taaaaaagaa ggaatttgga caaaggcaaa
     1621 ccgtctaatg taattaacca acgaaaaagc tttccggact tttaaatgct aactgttttt
     1681 ccccttcctg tctaggaaaa tgctataaag ctcaaattag ttaggaatga cttatacgtt
     1741 ttgttttgaa tacctaagag atactttttg gatatttata ttgccatatt cttacttgaa
     1801 tgctttgaat gactacatcc agttctgcac ctataccctc tggtgttgct ttttaacctt
     1861 cctggaatcc attttctaaa aaataaagac attttcagat ctgagagcta catctcaatg
     1921 tctgtggtta taattctgga caggataaat agctaaactt aatgtaggca aatgcagaga
     1981 catttatctg aaatgtagac ctctacactg agacttttct ggcatagtgg ctaaaacaag
     2041 atctacacat gcataaaaag ggacaatcac cttttcttca taaatataca gctttaggaa
     2101 tatttcacca ttctttgtag gacatagtag tccttgtctt tttttctcct gacattggaa
     2161 agatgtgcta attgaaactt gacttagtag gaacattgtg ccaactcaaa accttgattt
     2221 agtaaaaatc tcaatgttta gatcctttgt ccagtggtgg tgtttatcag ggaatgtatt
     2281 cagcttgctc agaaaaccaa aagggtatta aagccacaaa agcaaagaag aaaaaaaaaa
     2341 acttcccatg tttggatctt gttctagtta gaaaaattaa gttgaaattc ttggactttt
     2401 tcattcatga ggcaaatgct gtaatacctt cccctttgac aggtttggat tcttaacatt
     2461 actagtggta tttcaggaag tgacgttaca gttactttcc ttatagcggc taagtgtatt
     2521 aagttgaatg taacgatggt aatattaatt tgtttgaact gaggcccact actgattctt
     2581 tgacaaattg aattcttata tttaaataat tttatgggaa tgttccatca taatttctaa
     2641 atcatttata tatcaaggta gccttaattt gtatatgttt cagtacaatg agattttatt
     2701 gcctctggga tgctgtttag tttgtatttt gttgaacgtt tttatcctag gaagagaaac
     2761 ctatgacttg tgtacctaga tcatctgtta cattaaaaag ctgctctttc agcattagag
     2821 ctataaatga atgttacctt gtcgggaaac aatctaggtt ttagctgtat gagctatgtt
     2881 tattatggtg ctaatgttca gtagccacat ttgactaatg tctccattct ctgtgatgct
     2941 gtggctagca gcagagctcg ccagttcatg cctggacata ctgtcagggc tgggccctcc
     3001 agctagctcc tttggggttg agtccgtatc tttttgatgt ggaagtataa agcaagtatc
     3061 ttgatttcta aacccagcaa ttttagaatt gacctttatg agtgaagact tttggagctt
     3121 ttaaagacct tggcagtcat gatctcaaac caattaggag ctccaagctc ccttcccagg
     3181 taactgttgg gagcaatggc atcactgtat gcccttgtaa tggctggaag ggacatgatc
     3241 ttgtaagtag gaaagctgta actaaaaatt gtattgtttg cttattagcc atgtatctct
     3301 taaaattttg ttatgtttac aacgatgtac cttattggca acaagttatt agtttgatgt
     3361 ttaacaatag tgcctttagt aaattatttt acaactaaaa
//
