LOCUS       NM_001330246            3397 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens methionyl aminopeptidase 2 (METAP2), transcript
            variant 5, mRNA.
ACCESSION   NM_001330246 XM_005268583
VERSION     NM_001330246.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Santhosh Kumar,S., Naseri,N.N., Pather,S.R., Hallacli,E.,
            Ndayisaba,A., Buenaventura,C., Acosta,K., Roof,J., Fazelinia,H.,
            Spruce,L.A., Luk,K., Khurana,V., Rhoades,E. and Shalem,O.
  TITLE     Sequential CRISPR screening reveals partial NatB inhibition as a
            strategy to mitigate alpha-synuclein levels in human neurons
  JOURNAL   Sci Adv 10 (6), eadj4767 (2024)
   PUBMED   38335281
  REMARK    GeneRIF: Sequential CRISPR screening reveals partial NatB
            inhibition as a strategy to mitigate alpha-synuclein levels in
            human neurons.
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 3397)
  AUTHORS   Xie,J., Rice,M.A., Chen,Z., Cheng,Y., Hsu,E.C., Chen,M., Song,G.,
            Cui,L., Zhou,K., Castillo,J.B., Zhang,C.A., Shen,B., Chin,F.T.,
            Kunder,C.A., Brooks,J.D., Stoyanova,T. and Rao,J.
  TITLE     In Vivo Imaging of Methionine Aminopeptidase II for Prostate Cancer
            Risk Stratification
  JOURNAL   Cancer Res 81 (9), 2510-2521 (2021)
   PUBMED   33637565
  REMARK    GeneRIF: In Vivo Imaging of Methionine Aminopeptidase II for
            Prostate Cancer Risk Stratification.
REFERENCE   4  (bases 1 to 3397)
  AUTHORS   He,W.P., Guo,Y.Y., Yang,G.P., Lai,H.L., Sun,T.T., Zhang,Z.W.,
            Ouyang,L.L., Zheng,Y., Tian,L.M., Li,X.H., You,Z.S., Xie,D. and
            Yang,G.F.
  TITLE     CHD1L promotes EOC cell invasiveness and metastasis via the
            regulation of METAP2
  JOURNAL   Int J Med Sci 17 (15), 2387-2395 (2020)
   PUBMED   32922205
  REMARK    GeneRIF: CHD1L promotes EOC cell invasiveness and metastasis via
            the regulation of METAP2.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3397)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   6  (bases 1 to 3397)
  AUTHORS   Joharapurkar,A.A., Dhanesha,N.A. and Jain,M.R.
  TITLE     Inhibition of the methionine aminopeptidase 2 enzyme for the
            treatment of obesity
  JOURNAL   Diabetes Metab Syndr Obes 7, 73-84 (2014)
   PUBMED   24611021
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 3397)
  AUTHORS   Arfin,S.M., Kendall,R.L., Hall,L., Weaver,L.H., Stewart,A.E.,
            Matthews,B.W. and Bradshaw,R.A.
  TITLE     Eukaryotic methionyl aminopeptidases: two classes of
            cobalt-dependent enzymes
  JOURNAL   Proc Natl Acad Sci U S A 92 (17), 7714-7718 (1995)
   PUBMED   7644482
REFERENCE   8  (bases 1 to 3397)
  AUTHORS   Prigmore,E., Ahmed,S., Best,A., Kozma,R., Manser,E., Segal,A.W. and
            Lim,L.
  TITLE     A 68-kDa kinase and NADPH oxidase component p67phox are targets for
            Cdc42Hs and Rac1 in neutrophils
  JOURNAL   J Biol Chem 270 (18), 10717-10722 (1995)
   PUBMED   7738010
REFERENCE   9  (bases 1 to 3397)
  AUTHORS   Li,X. and Chang,Y.H.
  TITLE     Molecular cloning of a human complementary DNA encoding an
            initiation factor 2-associated protein (p67)
  JOURNAL   Biochim Biophys Acta 1260 (3), 333-336 (1995)
   PUBMED   7873610
REFERENCE   10 (bases 1 to 3397)
  AUTHORS   Ray,M.K., Chakraborty,A., Datta,B., Chattopadhyay,A., Saha,D.,
            Bose,A., Kinzy,T.G., Wu,S., Hileman,R.E., Merrick,W.C. et al.
  TITLE     Characteristics of the eukaryotic initiation factor 2 associated
            67-kDa polypeptide
  JOURNAL   Biochemistry 32 (19), 5151-5159 (1993)
   PUBMED   8098621
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from U13261.1, AK091730.1,
            AC018475.32 and CA450598.1.
            
            On May 31, 2019 this sequence version replaced NM_001330246.1.
            
            Summary: The protein encoded by this gene is a member of the
            methionyl aminopeptidase family. The encoded protein functions both
            by protecting the alpha subunit of eukaryotic initiation factor 2
            from inhibitory phosphorylation and by removing the amino-terminal
            methionine residue from nascent proteins. Increased expression of
            this gene is associated with various forms of cancer, and the
            anti-cancer drugs fumagillin and ovalicin inhibit the protein by
            irreversibly binding to its active site. Inhibitors of this gene
            have also been shown to be effective for the treatment of obesity.
            A pseudogene of this gene is located on chromosome 2. Several
            transcript variants encoding different isoforms have been found for
            this gene. [provided by RefSeq, Nov 2015].
            
            Transcript Variant: This variant (5) uses an alternate in-frame
            splice junction in the 5' coding region compared to variant 1. The
            resulting isoform (4) has the same N- and C-termini but is one
            amino acid shorter compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.205314.1,
                                           SRR1803615.114090.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-18                U13261.1           7-24
            19-284              AK091730.1         1-266
            285-1593            U13261.1           294-1602
            1594-2726           AC018475.32        50122-51254         c
            2727-3397           CA450598.1         15-685              c
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q22"
     gene            1..3397
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /note="methionyl aminopeptidase 2"
                     /db_xref="GeneID:10988"
                     /db_xref="HGNC:HGNC:16672"
                     /db_xref="MIM:601870"
     exon            1..179
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     CDS             29..1462
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /EC_number="3.4.11.18"
                     /note="isoform 4 is encoded by transcript variant 5;
                     initiation factor 2-associated 67 kDa glycoprotein;
                     peptidase M 2; eIF-2-associated p67 homolog; testicular
                     tissue protein Li 17; methionine aminopeptidase 2"
                     /codon_start=1
                     /product="methionine aminopeptidase 2 isoform 4"
                     /protein_id="NP_001317175.1"
                     /db_xref="CCDS:CCDS81724.1"
                     /db_xref="GeneID:10988"
                     /db_xref="HGNC:HGNC:16672"
                     /db_xref="MIM:601870"
                     /translation="MAGVEEVAASGSHLNGDLDPDDREEGAASTAEEAAKKKRRKKKK
                     SKGPSAGEQEPDKESGASVDEVARQLERSALEDKERDEDDEDGDGDGDGATGKKKKKK
                     KKKRGPKVQTDPPSVPICDLYPNGVFPKGQECEYPPTQDGRTAAWRTTSEEKKALDQA
                     SEEIWNDFREAAEAHRQVRKYVMSWIKPGMTMIEICEKLEDCSRKLIKENGLNAGLAF
                     PTGCSLNNCAAHYTPNAGDTTVLQYDDICKIDFGTHISGRIIDCAFTVTFNPKYDTLL
                     KAVKDATNTGIKCAGIDVRLCDVGEAIQEVMESYEVEIDGKTYQVKPIRNLNGHSIGQ
                     YRIHAGKTVPIVKGGEATRMEEGEVYAIETFGSTGKGVVHDDMECSHYMKNFDVGHVP
                     IRLPRTKHLLNVINENFGTLAFCRRWLDRLGESKYLMALKNLCDLGIVDPYPPLCDIK
                     GSYTAQFEHTILLRPTCKEVVSRGDDY"
     exon            180..284
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            285..350
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            351..453
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            454..615
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            616..797
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            798..892
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            893..989
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            990..1093
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1094..1209
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1210..3397
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1879..1884
                     /regulatory_class="polyA_signal_sequence"
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /note="hexamer: AATAAA"
     polyA_site      1901
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /note="major polyA site"
     regulatory      3375..3380
                     /regulatory_class="polyA_signal_sequence"
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /note="hexamer: AGTAAA"
     polyA_site      3397
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
ORIGIN      
        1 ctcattccct cgcgctctct cgggcaacat ggcgggtgtg gaggaggtag cggcctccgg
       61 gagccacctg aatggcgacc tggatccaga cgacagggaa gaaggagctg cctctacggc
      121 tgaggaagca gccaagaaaa aaagacgaaa gaagaagaag agcaaagggc cttctgcagg
      181 ggaacaggaa cctgataaag aatcaggagc ctcagtggat gaagtagcaa gacagttgga
      241 aagatcagca ttggaagata aagaaagaga tgaagatgat gaagatggag atggcgatgg
      301 agatggagca actggaaaga agaagaaaaa gaagaagaag aagagaggac caaaagttca
      361 aacagaccct ccctcagttc caatatgtga cctgtatcct aatggtgtat ttcccaaagg
      421 acaagaatgc gaatacccac ccacacaaga tgggcgaaca gctgcttgga gaactacaag
      481 tgaagaaaag aaagcattag atcaggcaag tgaagagatt tggaatgatt ttcgagaagc
      541 tgcagaagca catcgacaag ttagaaaata cgtaatgagc tggatcaagc ctgggatgac
      601 aatgatagaa atctgtgaaa agttggaaga ctgttcacgc aagttaataa aagagaatgg
      661 attaaatgca ggcctggcat ttcctactgg atgttctctc aataattgtg ctgcccatta
      721 tactcccaat gccggtgaca caacagtatt acagtatgat gacatctgta aaatagactt
      781 tggaacacat ataagtggta ggattattga ctgtgctttt actgtcactt ttaatcccaa
      841 atatgatacg ttattaaaag ctgtaaaaga tgctactaac actggaataa agtgtgctgg
      901 aattgatgtt cgtctgtgtg atgttggtga ggccatccaa gaagttatgg agtcctatga
      961 agttgaaata gatgggaaga catatcaagt gaaaccaatc cgtaatctaa atggacattc
     1021 aattgggcaa tatagaatac atgctggaaa aacagtgccg attgtgaaag gaggggaggc
     1081 aacaagaatg gaggaaggag aagtatatgc aattgaaacc tttggtagta caggaaaagg
     1141 tgttgttcat gatgatatgg aatgttcaca ttacatgaaa aattttgatg ttggacatgt
     1201 gccaataagg cttccaagaa caaaacactt gttaaatgtc atcaatgaaa actttggaac
     1261 ccttgccttc tgccgcagat ggctggatcg cttgggagaa agtaaatact tgatggctct
     1321 gaagaatctg tgtgacttgg gcattgtaga tccatatcca ccattatgtg acattaaagg
     1381 atcatataca gcgcaatttg aacataccat cctgttgcgt ccaacatgta aagaagttgt
     1441 cagcagagga gatgactatt aaacttagtc caaagccacc tcaacacctt tattttctga
     1501 gctttgttgg aaaacatgat accagaatta atttgccaca tgttgtctgt tttaacagtg
     1561 gacccatgta atacttttat ccatgtttaa aaaagaagga atttggacaa aggcaaaccg
     1621 tctaatgtaa ttaaccaacg aaaaagcttt ccggactttt aaatgctaac tgtttttccc
     1681 cttcctgtct aggaaaatgc tataaagctc aaattagtta ggaatgactt atacgttttg
     1741 ttttgaatac ctaagagata ctttttggat atttatattg ccatattctt acttgaatgc
     1801 tttgaatgac tacatccagt tctgcaccta taccctctgg tgttgctttt taaccttcct
     1861 ggaatccatt ttctaaaaaa taaagacatt ttcagatctg agagctacat ctcaatgtct
     1921 gtggttataa ttctggacag gataaatagc taaacttaat gtaggcaaat gcagagacat
     1981 ttatctgaaa tgtagacctc tacactgaga cttttctggc atagtggcta aaacaagatc
     2041 tacacatgca taaaaaggga caatcacctt ttcttcataa atatacagct ttaggaatat
     2101 ttcaccattc tttgtaggac atagtagtcc ttgtcttttt ttctcctgac attggaaaga
     2161 tgtgctaatt gaaacttgac ttagtaggaa cattgtgcca actcaaaacc ttgatttagt
     2221 aaaaatctca atgtttagat cctttgtcca gtggtggtgt ttatcaggga atgtattcag
     2281 cttgctcaga aaaccaaaag ggtattaaag ccacaaaagc aaagaagaaa aaaaaaaact
     2341 tcccatgttt ggatcttgtt ctagttagaa aaattaagtt gaaattcttg gactttttca
     2401 ttcatgaggc aaatgctgta ataccttccc ctttgacagg tttggattct taacattact
     2461 agtggtattt caggaagtga cgttacagtt actttcctta tagcggctaa gtgtattaag
     2521 ttgaatgtaa cgatggtaat attaatttgt ttgaactgag gcccactact gattctttga
     2581 caaattgaat tcttatattt aaataatttt atgggaatgt tccatcataa tttctaaatc
     2641 atttatatat caaggtagcc ttaatttgta tatgtttcag tacaatgaga ttttattgcc
     2701 tctgggatgc tgtttagttt gtattttgtt gaacgttttt atcctaggaa gagaaaccta
     2761 tgacttgtgt acctagatca tctgttacat taaaaagctg ctctttcagc attagagcta
     2821 taaatgaatg ttaccttgtc gggaaacaat ctaggtttta gctgtatgag ctatgtttat
     2881 tatggtgcta atgttcagta gccacatttg actaatgtct ccattctctg tgatgctgtg
     2941 gctagcagca gagctcgcca gttcatgcct ggacatactg tcagggctgg gccctccagc
     3001 tagctccttt ggggttgagt ccgtatcttt ttgatgtgga agtataaagc aagtatcttg
     3061 atttctaaac ccagcaattt tagaattgac ctttatgagt gaagactttt ggagctttta
     3121 aagaccttgg cagtcatgat ctcaaaccaa ttaggagctc caagctccct tcccaggtaa
     3181 ctgttgggag caatggcatc actgtatgcc cttgtaatgg ctggaaggga catgatcttg
     3241 taagtaggaa agctgtaact aaaaattgta ttgtttgctt attagccatg tatctcttaa
     3301 aattttgtta tgtttacaac gatgtacctt attggcaaca agttattagt ttgatgttta
     3361 acaatagtgc ctttagtaaa ttattttaca actaaaa
//
