LOCUS       NM_001317182            3331 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens methionyl aminopeptidase 2 (METAP2), transcript
            variant 2, mRNA.
ACCESSION   NM_001317182
VERSION     NM_001317182.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Santhosh Kumar,S., Naseri,N.N., Pather,S.R., Hallacli,E.,
            Ndayisaba,A., Buenaventura,C., Acosta,K., Roof,J., Fazelinia,H.,
            Spruce,L.A., Luk,K., Khurana,V., Rhoades,E. and Shalem,O.
  TITLE     Sequential CRISPR screening reveals partial NatB inhibition as a
            strategy to mitigate alpha-synuclein levels in human neurons
  JOURNAL   Sci Adv 10 (6), eadj4767 (2024)
   PUBMED   38335281
  REMARK    GeneRIF: Sequential CRISPR screening reveals partial NatB
            inhibition as a strategy to mitigate alpha-synuclein levels in
            human neurons.
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 3331)
  AUTHORS   Xie,J., Rice,M.A., Chen,Z., Cheng,Y., Hsu,E.C., Chen,M., Song,G.,
            Cui,L., Zhou,K., Castillo,J.B., Zhang,C.A., Shen,B., Chin,F.T.,
            Kunder,C.A., Brooks,J.D., Stoyanova,T. and Rao,J.
  TITLE     In Vivo Imaging of Methionine Aminopeptidase II for Prostate Cancer
            Risk Stratification
  JOURNAL   Cancer Res 81 (9), 2510-2521 (2021)
   PUBMED   33637565
  REMARK    GeneRIF: In Vivo Imaging of Methionine Aminopeptidase II for
            Prostate Cancer Risk Stratification.
REFERENCE   4  (bases 1 to 3331)
  AUTHORS   He,W.P., Guo,Y.Y., Yang,G.P., Lai,H.L., Sun,T.T., Zhang,Z.W.,
            Ouyang,L.L., Zheng,Y., Tian,L.M., Li,X.H., You,Z.S., Xie,D. and
            Yang,G.F.
  TITLE     CHD1L promotes EOC cell invasiveness and metastasis via the
            regulation of METAP2
  JOURNAL   Int J Med Sci 17 (15), 2387-2395 (2020)
   PUBMED   32922205
  REMARK    GeneRIF: CHD1L promotes EOC cell invasiveness and metastasis via
            the regulation of METAP2.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3331)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   6  (bases 1 to 3331)
  AUTHORS   Joharapurkar,A.A., Dhanesha,N.A. and Jain,M.R.
  TITLE     Inhibition of the methionine aminopeptidase 2 enzyme for the
            treatment of obesity
  JOURNAL   Diabetes Metab Syndr Obes 7, 73-84 (2014)
   PUBMED   24611021
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 3331)
  AUTHORS   Arfin,S.M., Kendall,R.L., Hall,L., Weaver,L.H., Stewart,A.E.,
            Matthews,B.W. and Bradshaw,R.A.
  TITLE     Eukaryotic methionyl aminopeptidases: two classes of
            cobalt-dependent enzymes
  JOURNAL   Proc Natl Acad Sci U S A 92 (17), 7714-7718 (1995)
   PUBMED   7644482
REFERENCE   8  (bases 1 to 3331)
  AUTHORS   Prigmore,E., Ahmed,S., Best,A., Kozma,R., Manser,E., Segal,A.W. and
            Lim,L.
  TITLE     A 68-kDa kinase and NADPH oxidase component p67phox are targets for
            Cdc42Hs and Rac1 in neutrophils
  JOURNAL   J Biol Chem 270 (18), 10717-10722 (1995)
   PUBMED   7738010
REFERENCE   9  (bases 1 to 3331)
  AUTHORS   Li,X. and Chang,Y.H.
  TITLE     Molecular cloning of a human complementary DNA encoding an
            initiation factor 2-associated protein (p67)
  JOURNAL   Biochim Biophys Acta 1260 (3), 333-336 (1995)
   PUBMED   7873610
REFERENCE   10 (bases 1 to 3331)
  AUTHORS   Ray,M.K., Chakraborty,A., Datta,B., Chattopadhyay,A., Saha,D.,
            Bose,A., Kinzy,T.G., Wu,S., Hileman,R.E., Merrick,W.C. et al.
  TITLE     Characteristics of the eukaryotic initiation factor 2 associated
            67-kDa polypeptide
  JOURNAL   Biochemistry 32 (19), 5151-5159 (1993)
   PUBMED   8098621
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK300836.1 and AC018475.32.
            
            On May 31, 2019 this sequence version replaced NM_001317182.1.
            
            Summary: The protein encoded by this gene is a member of the
            methionyl aminopeptidase family. The encoded protein functions both
            by protecting the alpha subunit of eukaryotic initiation factor 2
            from inhibitory phosphorylation and by removing the amino-terminal
            methionine residue from nascent proteins. Increased expression of
            this gene is associated with various forms of cancer, and the
            anti-cancer drugs fumagillin and ovalicin inhibit the protein by
            irreversibly binding to its active site. Inhibitors of this gene
            have also been shown to be effective for the treatment of obesity.
            A pseudogene of this gene is located on chromosome 2. Several
            transcript variants encoding different isoforms have been found for
            this gene. [provided by RefSeq, Nov 2015].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice junction compared to variant 1. The resulting isoform (2)
            has the same N- and C-termini but is shorter compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK300836.1, SRR1803614.176514.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-363               AK300836.1         72-434
            364-364             AC018475.32        79332-79332         c
            365-1791            AK300836.1         436-1862
            1792-3331           AC018475.32        49451-50990         c
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q22"
     gene            1..3331
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /note="methionyl aminopeptidase 2"
                     /db_xref="GeneID:10988"
                     /db_xref="HGNC:HGNC:16672"
                     /db_xref="MIM:601870"
     exon            1..179
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     CDS             29..1396
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /EC_number="3.4.11.18"
                     /note="isoform 2 is encoded by transcript variant 2;
                     initiation factor 2-associated 67 kDa glycoprotein;
                     peptidase M 2; eIF-2-associated p67 homolog; testicular
                     tissue protein Li 17; methionine aminopeptidase 2"
                     /codon_start=1
                     /product="methionine aminopeptidase 2 isoform 2"
                     /protein_id="NP_001304111.1"
                     /db_xref="CCDS:CCDS81723.1"
                     /db_xref="GeneID:10988"
                     /db_xref="HGNC:HGNC:16672"
                     /db_xref="MIM:601870"
                     /translation="MAGVEEVAASGSHLNGDLDPDDREEGAASTAEEAAKKKRRKKKK
                     SKGPSAAGEQEPDKESGASVDEVARQLERSALEDKERDEDDEDGDGDGDGATGKKKKK
                     KKKKRGRQECEYPPTQDGRTAAWRTTSEEKKALDQASEEIWNDFREAAEAHRQVRKYV
                     MSWIKPGMTMIEICEKLEDCSRKLIKENGLNAGLAFPTGCSLNNCAAHYTPNAGDTTV
                     LQYDDICKIDFGTHISGRIIDCAFTVTFNPKYDTLLKAVKDATNTGIKCAGIDVRLCD
                     VGEAIQEVMESYEVEIDGKTYQVKPIRNLNGHSIGQYRIHAGKTVPIVKGGEATRMEE
                     GEVYAIETFGSTGKGVVHDDMECSHYMKNFDVGHVPIRLPRTKHLLNVINENFGTLAF
                     CRRWLDRLGESKYLMALKNLCDLGIVDPYPPLCDIKGSYTAQFEHTILLRPTCKEVVS
                     RGDDY"
     exon            180..287
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            288..353
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            354..387
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            388..549
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            550..731
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            732..826
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            827..923
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            924..1027
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1028..1143
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1144..3331
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1813..1818
                     /regulatory_class="polyA_signal_sequence"
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /note="hexamer: AATAAA"
     polyA_site      1835
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /note="major polyA site"
     regulatory      3309..3314
                     /regulatory_class="polyA_signal_sequence"
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
                     /note="hexamer: AGTAAA"
     polyA_site      3331
                     /gene="METAP2"
                     /gene_synonym="MAP2; MNPEP; p67eIF2"
ORIGIN      
        1 ctcattccct cgcgctctct cgggcaacat ggcgggtgtg gaggaggtag cggcctccgg
       61 gagccacctg aatggcgacc tggatccaga cgacagggaa gaaggagctg cctctacggc
      121 tgaggaagca gccaagaaaa aaagacgaaa gaagaagaag agcaaagggc cttctgcagc
      181 aggggaacag gaacctgata aagaatcagg agcctcagtg gatgaagtag caagacagtt
      241 ggaaagatca gcattggaag ataaagaaag agatgaagat gatgaagatg gagatggcga
      301 tggagatgga gcaactggaa agaagaagaa aaagaagaag aagaagagag gacgacaaga
      361 atgcgaatac ccacccacac aagatgggcg aacagctgct tggagaacta caagtgaaga
      421 aaagaaagca ttagatcagg caagtgaaga gatttggaat gattttcgag aagctgcaga
      481 agcacatcga caagttagaa aatacgtaat gagctggatc aagcctggga tgacaatgat
      541 agaaatctgt gaaaagttgg aagactgttc acgcaagtta ataaaagaga atggattaaa
      601 tgcaggcctg gcatttccta ctggatgttc tctcaataat tgtgctgccc attatactcc
      661 caatgccggt gacacaacag tattacagta tgatgacatc tgtaaaatag actttggaac
      721 acatataagt ggtaggatta ttgactgtgc ttttactgtc acttttaatc ccaaatatga
      781 tacgttatta aaagctgtaa aagatgctac taacactgga ataaagtgtg ctggaattga
      841 tgttcgtctg tgtgatgttg gtgaggccat ccaagaagtt atggagtcct atgaagttga
      901 aatagatggg aagacatatc aagtgaaacc aatccgtaat ctaaatggac attcaattgg
      961 gcaatataga atacatgctg gaaaaacagt gccgattgtg aaaggagggg aggcaacaag
     1021 aatggaggaa ggagaagtat atgcaattga aacctttggt agtacaggaa aaggtgttgt
     1081 tcatgatgat atggaatgtt cacattacat gaaaaatttt gatgttggac atgtgccaat
     1141 aaggcttcca agaacaaaac acttgttaaa tgtcatcaat gaaaactttg gaacccttgc
     1201 cttctgccgc agatggctgg atcgcttggg agaaagtaaa tacttgatgg ctctgaagaa
     1261 tctgtgtgac ttgggcattg tagatccata tccaccatta tgtgacatta aaggatcata
     1321 tacagcgcaa tttgaacata ccatcctgtt gcgtccaaca tgtaaagaag ttgtcagcag
     1381 aggagatgac tattaaactt agtccaaagc cacctcaaca cctttatttt ctgagctttg
     1441 ttggaaaaca tgataccaga attaatttgc cacatgttgt ctgttttaac agtggaccca
     1501 tgtaatactt ttatccatgt ttaaaaaaga aggaatttgg acaaaggcaa accgtctaat
     1561 gtaattaacc aacgaaaaag ctttccggac ttttaaatgc taactgtttt tccccttcct
     1621 gtctaggaaa atgctataaa gctcaaatta gttaggaatg acttatacgt tttgttttga
     1681 atacctaaga gatacttttt ggatatttat attgccatat tcttacttga atgctttgaa
     1741 tgactacatc cagttctgca cctataccct ctggtgttgc tttttaacct tcctggaatc
     1801 cattttctaa aaaataaaga cattttcaga tctgagagct acatctcaat gtctgtggtt
     1861 ataattctgg acaggataaa tagctaaact taatgtaggc aaatgcagag acatttatct
     1921 gaaatgtaga cctctacact gagacttttc tggcatagtg gctaaaacaa gatctacaca
     1981 tgcataaaaa gggacaatca ccttttcttc ataaatatac agctttagga atatttcacc
     2041 attctttgta ggacatagta gtccttgtct ttttttctcc tgacattgga aagatgtgct
     2101 aattgaaact tgacttagta ggaacattgt gccaactcaa aaccttgatt tagtaaaaat
     2161 ctcaatgttt agatcctttg tccagtggtg gtgtttatca gggaatgtat tcagcttgct
     2221 cagaaaacca aaagggtatt aaagccacaa aagcaaagaa gaaaaaaaaa aacttcccat
     2281 gtttggatct tgttctagtt agaaaaatta agttgaaatt cttggacttt ttcattcatg
     2341 aggcaaatgc tgtaatacct tcccctttga caggtttgga ttcttaacat tactagtggt
     2401 atttcaggaa gtgacgttac agttactttc cttatagcgg ctaagtgtat taagttgaat
     2461 gtaacgatgg taatattaat ttgtttgaac tgaggcccac tactgattct ttgacaaatt
     2521 gaattcttat atttaaataa ttttatggga atgttccatc ataatttcta aatcatttat
     2581 atatcaaggt agccttaatt tgtatatgtt tcagtacaat gagattttat tgcctctggg
     2641 atgctgttta gtttgtattt tgttgaacgt ttttatccta ggaagagaaa cctatgactt
     2701 gtgtacctag atcatctgtt acattaaaaa gctgctcttt cagcattaga gctataaatg
     2761 aatgttacct tgtcgggaaa caatctaggt tttagctgta tgagctatgt ttattatggt
     2821 gctaatgttc agtagccaca tttgactaat gtctccattc tctgtgatgc tgtggctagc
     2881 agcagagctc gccagttcat gcctggacat actgtcaggg ctgggccctc cagctagctc
     2941 ctttggggtt gagtccgtat ctttttgatg tggaagtata aagcaagtat cttgatttct
     3001 aaacccagca attttagaat tgacctttat gagtgaagac ttttggagct tttaaagacc
     3061 ttggcagtca tgatctcaaa ccaattagga gctccaagct cccttcccag gtaactgttg
     3121 ggagcaatgg catcactgta tgcccttgta atggctggaa gggacatgat cttgtaagta
     3181 ggaaagctgt aactaaaaat tgtattgttt gcttattagc catgtatctc ttaaaatttt
     3241 gttatgttta caacgatgta ccttattggc aacaagttat tagtttgatg tttaacaata
     3301 gtgcctttag taaattattt tacaactaaa a
//
