LOCUS       NM_005925               2297 bp    mRNA    linear   PRI 03-AUG-2024
DEFINITION  Homo sapiens meprin A subunit beta (MEP1B), transcript variant 1,
            mRNA.
ACCESSION   NM_005925
VERSION     NM_005925.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Siemsen,W., Halske,C., Behrens,H.M., Kruger,S., Becker-Pauly,C. and
            Rocken,C.
  TITLE     The putative pleiotropic functions of meprin beta in gastric cancer
  JOURNAL   Gastric Cancer 26 (4), 542-552 (2023)
   PUBMED   36976399
  REMARK    GeneRIF: The putative pleiotropic functions of meprin beta in
            gastric cancer.
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Linnert,M., Fritz,C., Jager,C., Schlenzig,D., Ramsbeck,D.,
            Kleinschmidt,M., Wermann,M., Demuth,H.U., Parthier,C. and
            Schilling,S.
  TITLE     Structure and Dynamics of Meprin beta in Complex with a
            Hydroxamate-Based Inhibitor
  JOURNAL   Int J Mol Sci 22 (11), 5651 (2021)
   PUBMED   34073350
  REMARK    GeneRIF: Structure and Dynamics of Meprin beta in Complex with a
            Hydroxamate-Based Inhibitor.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2297)
  AUTHORS   Menon,P.K., Koistinen,N.A., Iverfeldt,K. and Strom,A.L.
  TITLE     Phosphorylation of the amyloid precursor protein (APP) at Ser-675
            promotes APP processing involving meprin beta
  JOURNAL   J Biol Chem 294 (47), 17768-17776 (2019)
   PUBMED   31604820
  REMARK    GeneRIF: As meprin beta cleavage of APP has been shown to result in
            formation of highly aggregation-prone, truncated Abeta2-40/42
            peptides, enhanced APP processing by this enzyme could contribute
            to Alzheimer's disease pathology.
REFERENCE   4  (bases 1 to 2297)
  AUTHORS   Schaffler,H., Li,W., Helm,O., Kruger,S., Boger,C., Peters,F.,
            Rocken,C., Sebens,S., Lucius,R., Becker-Pauly,C. and Arnold,P.
  TITLE     The cancer-associated meprin beta variant G32R provides an
            additional activation site and promotes cancer cell invasion
  JOURNAL   J Cell Sci 132 (11) (2019)
   PUBMED   31076514
  REMARK    GeneRIF: The authors found that increased meprin beta G32R activity
            at the cell surface reduces cell proliferation, but increases cell
            invasion.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2297)
  AUTHORS   Wichert,R., Ermund,A., Schmidt,S., Schweinlin,M., Ksiazek,M.,
            Arnold,P., Knittler,K., Wilkens,F., Potempa,B., Rabe,B.,
            Stirnberg,M., Lucius,R., Bartsch,J.W., Nikolaus,S.,
            Falk-Paulsen,M., Rosenstiel,P., Metzger,M., Rose-John,S.,
            Potempa,J., Hansson,G.C., Dempsey,P.J. and Becker-Pauly,C.
  TITLE     Mucus Detachment by Host Metalloprotease Meprin beta Requires
            Shedding of Its Inactive Pro-form, which Is Abrogated by the
            Pathogenic Protease RgpB
  JOURNAL   Cell Rep 21 (8), 2090-2103 (2017)
   PUBMED   29166602
  REMARK    GeneRIF: This secreted cysteine protease potently converts
            membrane-bound meprin beta into its active form, impairing meprin
            beta shedding and its function as a mucus-detaching protease
REFERENCE   6  (bases 1 to 2297)
  AUTHORS   Bankus,J.M. and Bond,J.S.
  TITLE     Expression and distribution of meprin protease subunits in mouse
            intestine
  JOURNAL   Arch Biochem Biophys 331 (1), 87-94 (1996)
   PUBMED   8660687
REFERENCE   7  (bases 1 to 2297)
  AUTHORS   Bond,J.S., Rojas,K., Overhauser,J., Zoghbi,H.Y. and Jiang,W.
  TITLE     The structural genes, MEP1A and MEP1B, for the alpha and beta
            subunits of the metalloendopeptidase meprin map to human
            chromosomes 6p and 18q, respectively
  JOURNAL   Genomics 25 (1), 300-303 (1995)
   PUBMED   7774936
REFERENCE   8  (bases 1 to 2297)
  AUTHORS   Yamaguchi,T., Fukase,M., Sugimoto,T., Kido,H. and Chihara,K.
  TITLE     Purification of meprin from human kidney and its role in
            parathyroid hormone degradation
  JOURNAL   Biol Chem Hoppe Seyler 375 (12), 821-824 (1994)
   PUBMED   7710697
REFERENCE   9  (bases 1 to 2297)
  AUTHORS   Kaushal,G.P., Walker,P.D. and Shah,S.V.
  TITLE     An old enzyme with a new function: purification and
            characterization of a distinct matrix-degrading metalloproteinase
            in rat kidney cortex and its identification as meprin
  JOURNAL   J Cell Biol 126 (5), 1319-1327 (1994)
   PUBMED   8063866
REFERENCE   10 (bases 1 to 2297)
  AUTHORS   Dumermuth,E., Eldering,J.A., Grunberg,J., Jiang,W. and Sterchi,E.E.
  TITLE     Cloning of the PABA peptide hydrolase alpha subunit (PPH alpha)
            from human small intestine and its expression in COS-1 cells
  JOURNAL   FEBS Lett 335 (3), 367-375 (1993)
   PUBMED   8262185
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AY695931.1 and X81333.1.
            
            On May 17, 2019 this sequence version replaced NM_005925.2.
            
            Summary: Meprins are multidomain zinc metalloproteases that are
            highly expressed in mammalian kidney and intestinal brush border
            membranes, and in leukocytes and certain cancer cells. They are
            involved in the hydrolysis of a variety of peptide and protein
            substrates, and have been implicated in cancer and intestinal
            inflammation. Mature meprins are oligomers of evolutionarily
            related, but separately encoded alpha and/or beta subunits.
            Homooligomers of alpha subunit are secreted, whereas, oligomers
            containing the beta subunit are plasma membrane-bound. This gene
            encodes the beta subunit. Targeted disruption of this gene in mice
            affects embryonic viability, renal gene expression profiles, and
            distribution of the membrane-associated alpha subunit in kidney and
            intestine. [provided by RefSeq, Oct 2011].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY695931.1, BC144244.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2144835
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000269202.11/ ENSP00000269202.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-991               AY695931.1         27-1017
            992-2115            X81333.1           1006-2129
            2116-2297           AY695931.1         2142-2323
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q12.1"
     gene            1..2297
                     /gene="MEP1B"
                     /note="meprin A subunit beta"
                     /db_xref="GeneID:4225"
                     /db_xref="HGNC:HGNC:7020"
                     /db_xref="MIM:600389"
     exon            1..95
                     /gene="MEP1B"
                     /inference="alignment:Splign:2.1.0"
     CDS             33..2138
                     /gene="MEP1B"
                     /EC_number="3.4.24.63"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; endopeptidase-2; PABA peptide hydrolase; PPH
                     beta; N-benzoyl-L-tyrosyl-p-amino-benzoic acid hydrolase
                     beta subunit; N-benzoyl-L-tyrosyl-P-amino-benzoic acid
                     hydrolase subunit beta; meprin B; meprin A, beta"
                     /codon_start=1
                     /product="meprin A subunit beta isoform 1 precursor"
                     /protein_id="NP_005916.2"
                     /db_xref="CCDS:CCDS45846.1"
                     /db_xref="GeneID:4225"
                     /db_xref="HGNC:HGNC:7020"
                     /db_xref="MIM:600389"
                     /translation="MDLWNLSWFLFLDALLVISGLATPENFDVDGGMDQDIFDINEGL
                     GLDLFEGDIRLDRAQIRNSIIGEKYRWPHTIPYVLEDSLEMNAKGVILNAFERYRLKT
                     CIDFKPWAGETNYISVFKGSGCWSSVGNRRVGKQELSIGANCDRIATVQHEFLHALGF
                     WHEQSRSDRDDYVRIMWDRILSGREHNFNTYSDDISDSLNVPYDYTSVMHYSKTAFQN
                     GTEPTIVTRISDFEDVIGQRMDFSDSDLLKLNQLYNCSSSLSFMDSCSFELENVCGMI
                     QSSGDNADWQRVSQVPRGPESDHSNMGQCQGSGFFMHFDSSSVNVGATAVLESRTLYP
                     KRGFQCLQFYLYNSGSESDQLNIYIREYSADNVDGNLTLVEEIKEIPTGSWQLYHVTL
                     KVTKKFRVVFEGRKGSGASLGGLSIDDINLSETRCPHHIWHIRNFTQFIGSPNGTLYS
                     PPFYSSKGYAFQIYLNLAHVTNAGIYFHLISGANDDQLQWPCPWQQATMTLLDQNPDI
                     RQRMSNQRSITTDPFMTTDNGNYFWDRPSKVGTVALFSNGTQFRRGGGYGTSAFITHE
                     RLKSRDFIKGDDVYILLTVEDISHLNSTQIQLTPAPSVQDLCSKTTCKNDGVCTVRDG
                     KAECRCQSGEDWWYMGERCEKRGSTRDTIVIAVSSTVAVFALMLIITLVSVYCTRKKY
                     RERMSSNRPNLTPQNQHAF"
     exon            96..114
                     /gene="MEP1B"
                     /inference="alignment:Splign:2.1.0"
     exon            115..159
                     /gene="MEP1B"
                     /inference="alignment:Splign:2.1.0"
     exon            160..203
                     /gene="MEP1B"
                     /inference="alignment:Splign:2.1.0"
     exon            204..282
                     /gene="MEP1B"
                     /inference="alignment:Splign:2.1.0"
     exon            283..400
                     /gene="MEP1B"
                     /inference="alignment:Splign:2.1.0"
     exon            401..579
                     /gene="MEP1B"
                     /inference="alignment:Splign:2.1.0"
     exon            580..798
                     /gene="MEP1B"
                     /inference="alignment:Splign:2.1.0"
     exon            799..951
                     /gene="MEP1B"
                     /inference="alignment:Splign:2.1.0"
     exon            952..1167
                     /gene="MEP1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1168..1611
                     /gene="MEP1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1612..1791
                     /gene="MEP1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1792..1918
                     /gene="MEP1B"
                     /inference="alignment:Splign:2.1.0"
     exon            1919..2123
                     /gene="MEP1B"
                     /inference="alignment:Splign:2.1.0"
     exon            2124..2297
                     /gene="MEP1B"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2269..2274
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MEP1B"
                     /note="hexamer: TATAAA"
     polyA_site      2297
                     /gene="MEP1B"
                     /note="major polyA site"
ORIGIN      
        1 atagttagct actttcaact ggaagctaca acatggattt atggaatctg tcttggtttc
       61 tgttcttgga tgctcttctc gtgatttctg gcttggcaac tccagaaaac tttgatgtag
      121 atggcggaat ggaccaggac atatttgata tcaatgaagg tttgggactg gatctttttg
      181 agggtgacat cagacttgat agggcacaaa ttagaaattc catcattgga gaaaagtata
      241 gatggcctca taccattcca tatgttctag aagatagctt ggaaatgaat gctaagggag
      301 ttatcctcaa tgcatttgaa cgttatcgcc ttaaaacatg tattgacttt aagccttggg
      361 ctggagaaac aaactatata tcagtgttca agggcagtgg ctgctggtct tcagtaggaa
      421 ataggcgggt tgggaagcaa gaactttcca tcggggcaaa ctgtgaccga atagcaacag
      481 ttcaacacga gttcctccac gctctgggat tctggcatga gcagtcgcgt tctgaccggg
      541 atgactatgt caggataatg tgggacagaa ttctgtcagg cagagagcac aattttaaca
      601 cctatagtga cgatatatca gattccctga atgttcccta tgattacact tcagtaatgc
      661 actacagtaa aactgcattc caaaatggaa cagagccgac aattgtcaca agaatctcag
      721 actttgagga tgtgatcggc caacgaatgg atttcagtga ctctgatctc ctaaagttga
      781 atcaactgta taactgctcc tcttccttga gttttatgga ctcgtgcagt tttgaactgg
      841 aaaatgtgtg tggcatgatc caaagttcag gagataatgc tgactggcaa cgggtttcac
      901 aggttcccag ggggccagag agtgatcact ccaacatggg ccagtgccaa ggttctggtt
      961 tcttcatgca tttcgatagc agctctgtaa atgtgggggc cacagcagtg ctggaaagta
     1021 gaacgctgta ccctaaaaga ggatttcagt gcctgcaatt ttacttatat aacagtggca
     1081 gtgaaagtga tcaactgaac atctatatca gggagtattc tgcagacaat gtggatggca
     1141 atttaaccct tgtggaagaa ataaaagaaa tacccactgg gagctggcaa ctttatcatg
     1201 taacattgaa agtgaccaag aagtttagag tggtgtttga aggacgcaaa ggctctggtg
     1261 catcactggg tggtctgtct attgatgaca tcaatctttc ggaaacacgg tgccctcatc
     1321 atatctggca tataaggaat ttcacacagt tcattggcag cccaaatgga actctgtata
     1381 gccctccatt ttactcttct aaaggttatg cctttcagat ttacttaaat ctagcccatg
     1441 tgactaatgc agggatatat ttccacttga tctctggagc caatgatgat caattacagt
     1501 ggccatgtcc ttggcaacaa gccacaatga cacttttgga tcaaaatcct gacattcgac
     1561 agcgtatgtc caatcagcgg agtataacta cagacccatt tatgaccacc gataatggaa
     1621 actatttctg ggacaggcct tctaaagtgg gaacagtggc tttgttctct aatggaactc
     1681 agtttagaag aggtgggggc tatggaacca gtgcctttat aacccacgaa aggctgaaaa
     1741 gcagagattt tataaaagga gatgatgttt atatcctact gacagtggaa gacatatctc
     1801 acctcaactc tacacaaatc cagctaacac cagcccctag tgttcaagac ctctgctcaa
     1861 aaaccacctg taaaaatgac ggtgtctgca ctgttcgaga tggcaaagct gagtgcaggt
     1921 gccagtcagg ggaagactgg tggtacatgg gagaaaggtg tgaaaagaga ggctccaccc
     1981 gagacaccat agtcattgct gtttcatcta ctgttgctgt gtttgccttg atgctgatca
     2041 tcacccttgt cagtgtctat tgcaccagga agaaatatcg tgaaaggatg agctcaaatc
     2101 gaccaaattt gactccgcaa aatcagcatg ctttttgaag attaactcga caatatggcc
     2161 agctaatgaa attaaaaagg attcttcatc atggatttcg cctaagtgat attacagcca
     2221 cctcattctt ctaaaagtgg atatttttct gtaaatagct ggaaatatta taaatcacta
     2281 ttttggtcaa agtcaaa
//
