LOCUS       NM_033316               1676 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens melanotransferrin (MELTF), transcript variant 2, mRNA.
ACCESSION   NM_033316
VERSION     NM_033316.4
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Shein,M., Hitzenberger,M., Cheng,T.C., Rout,S.R., Leitl,K.D.,
            Sato,Y., Zacharias,M., Sakata,E. and Schutz,A.K.
  TITLE     Characterizing ATP processing by the AAA+ protein p97 at the atomic
            level
  JOURNAL   Nat Chem 16 (3), 363-372 (2024)
   PUBMED   38326645
  REMARK    GeneRIF: Characterizing ATP processing by the AAA+ protein p97 at
            the atomic level.
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Zhang,L. and Shi,L.
  TITLE     The E2F1/MELTF axis fosters the progression of lung adenocarcinoma
            by regulating the Notch signaling pathway
  JOURNAL   Mutat Res 827, 111837 (2023)
   PUBMED   37820570
  REMARK    GeneRIF: The E2F1/MELTF axis fosters the progression of lung
            adenocarcinoma by regulating the Notch signaling pathway.
REFERENCE   3  (bases 1 to 1676)
  AUTHORS   Mao,Y., Wang,M., Xiong,Y., Wen,X., Zhang,M., Ma,L. and Zhang,Y.
  TITLE     MELTF Might Regulate Ferroptosis, Pyroptosis, and Autophagy in
            Platelet-Rich Plasma-Mediated Endometrial Epithelium Regeneration
  JOURNAL   Reprod Sci 30 (5), 1506-1520 (2023)
   PUBMED   36303086
  REMARK    GeneRIF: MELTF Might Regulate Ferroptosis, Pyroptosis, and
            Autophagy in Platelet-Rich Plasma-Mediated Endometrial Epithelium
            Regeneration.
REFERENCE   4  (bases 1 to 1676)
  AUTHORS   Lim,S.H., Snider,J., Birimberg-Schwartz,L., Ip,W., Serralha,J.C.,
            Botelho,H.M., Lopes-Pacheco,M., Pinto,M.C., Moutaoufik,M.T.,
            Zilocchi,M., Laselva,O., Esmaeili,M., Kotlyar,M., Lyakisheva,A.,
            Tang,P., Lopez Vazquez,L., Akula,I., Aboualizadeh,F., Wong,V.,
            Grozavu,I., Opacak-Bernardi,T., Yao,Z., Mendoza,M., Babu,M.,
            Jurisica,I., Gonska,T., Bear,C.E., Amaral,M.D. and Stagljar,I.
  TITLE     CFTR interactome mapping using the mammalian membrane two-hybrid
            high-throughput screening system
  JOURNAL   Mol Syst Biol 18 (2), e10629 (2022)
   PUBMED   35156780
REFERENCE   5  (bases 1 to 1676)
  AUTHORS   Valsecchi,V., Laudati,G., Cuomo,O., Sirabella,R., Del Prete,A.,
            Annunziato,L. and Pignataro,G.
  TITLE     The hypoxia sensitive metal transcription factor MTF-1 activates
            NCX1 brain promoter and participates in remote postconditioning
            neuroprotection in stroke
  JOURNAL   Cell Death Dis 12 (5), 423 (2021)
   PUBMED   33931586
  REMARK    GeneRIF: The hypoxia sensitive metal transcription factor MTF-1
            activates NCX1 brain promoter and participates in remote
            postconditioning neuroprotection in stroke.
            Erratum:[Cell Death Dis. 2023 Jul 31;14(7):484. doi:
            10.1038/s41419-023-05942-6.. PMID: 37524689]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1676)
  AUTHORS   Garratt,R.C. and Jhoti,H.
  TITLE     A molecular model for the tumour-associated antigen, p97, suggests
            a Zn-binding function
  JOURNAL   FEBS Lett 305 (1), 55-61 (1992)
   PUBMED   1633859
REFERENCE   7  (bases 1 to 1676)
  AUTHORS   Baker,E.N., Baker,H.M., Smith,C.A., Stebbins,M.R., Kahn,M.,
            Hellstrom,K.E. and Hellstrom,I.
  TITLE     Human melanotransferrin (p97) has only one functional iron-binding
            site
  JOURNAL   FEBS Lett 298 (2-3), 215-218 (1992)
   PUBMED   1544447
REFERENCE   8  (bases 1 to 1676)
  AUTHORS   Sciot,R., de Vos,R., van Eyken,P., van der Steen,K., Moerman,P. and
            Desmet,V.J.
  TITLE     In situ localization of melanotransferrin (melanoma-associated
            antigen P97) in human liver. A light- and electronmicroscopic
            immunohistochemical study
  JOURNAL   Liver 9 (2), 110-119 (1989)
   PUBMED   2540389
REFERENCE   9  (bases 1 to 1676)
  AUTHORS   Furukawa,K.S., Furukawa,K., Real,F.X., Old,L.J. and Lloyd,K.O.
  TITLE     A unique antigenic epitope of human melanoma is carried on the
            common melanoma glycoprotein gp95/p97
  JOURNAL   J Exp Med 169 (2), 585-590 (1989)
   PUBMED   2463331
REFERENCE   10 (bases 1 to 1676)
  AUTHORS   Rose,T.M., Plowman,G.D., Teplow,D.B., Dreyer,W.J., Hellstrom,K.E.
            and Brown,J.P.
  TITLE     Primary structure of the human melanoma-associated antigen p97
            (melanotransferrin) deduced from the mRNA sequence
  JOURNAL   Proc Natl Acad Sci U S A 83 (5), 1261-1265 (1986)
   PUBMED   2419904
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC068302.18, AU099352.1 and
            BC071910.1.
            
            On May 31, 2019 this sequence version replaced NM_033316.3.
            
            Summary: The protein encoded by this gene is a cell-surface
            glycoprotein found on melanoma cells. The protein shares sequence
            similarity and iron-binding properties with members of the
            transferrin superfamily. The importance of the iron binding
            function has not yet been identified. This gene resides in the same
            region of chromosome 3 as members of the transferrin superfamily.
            Alternative splicing results in two transcript variants. [provided
            by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) differs from variant 1 in the
            central coding region, resulting in a frameshift. The encoded
            isoform (2) has a truncated and distinct C-terminus and lacks the
            propeptide compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC002623.2, SRR1163655.414674.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 AC068302.18        41224-41225         c
            3-302               AU099352.1         1-300
            303-1056            BC071910.1         261-1014
            1057-1676           BC071910.1         1016-1635
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q29"
     gene            1..1676
                     /gene="MELTF"
                     /gene_synonym="CD228; MAP97; MFI2; MTf; MTF1"
                     /note="melanotransferrin"
                     /db_xref="GeneID:4241"
                     /db_xref="HGNC:HGNC:7037"
                     /db_xref="MIM:155750"
     exon            1..164
                     /gene="MELTF"
                     /gene_synonym="CD228; MAP97; MFI2; MTf; MTF1"
                     /inference="alignment:Splign:2.1.0"
     CDS             116..1024
                     /gene="MELTF"
                     /gene_synonym="CD228; MAP97; MFI2; MTf; MTF1"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; membrane-bound transferrin-like protein;
                     antigen p97 (melanoma associated) identified by monoclonal
                     antibodies 133.2 and 96.5"
                     /codon_start=1
                     /product="melanotransferrin isoform 2 precursor"
                     /protein_id="NP_201573.1"
                     /db_xref="CCDS:CCDS3326.1"
                     /db_xref="GeneID:4241"
                     /db_xref="HGNC:HGNC:7037"
                     /db_xref="MIM:155750"
                     /translation="MRGPSGALWLLLALRTVLGGMEVRWCATSDPEQHKCGNMSEAFR
                     EAGIQPSLLCVRGTSADHCVQLIAAQEADAITLDGGAIYEAGKEHGLKPVVGEVYDQE
                     VGTSYYAVAVVRRSSHVTIDTLKGVKSCHTGINRTVGWNVPVGYLVESGRLSVMGCDV
                     LKAVSDYFGGSCVPGAGETSYSESLCRLCRGDSSGEGVCDKSPLERYYDYSGAFRCLA
                     EGAGDVAFVKHSTVLENTDESPSRRQTWTRSEEEEGECPAHEEARRTMRSSAGQAWKW
                     APVHRPQDESDKGEFGKRAKSRDMLG"
     exon            165..319
                     /gene="MELTF"
                     /gene_synonym="CD228; MAP97; MFI2; MTf; MTF1"
                     /inference="alignment:Splign:2.1.0"
     exon            320..419
                     /gene="MELTF"
                     /gene_synonym="CD228; MAP97; MFI2; MTf; MTF1"
                     /inference="alignment:Splign:2.1.0"
     exon            420..602
                     /gene="MELTF"
                     /gene_synonym="CD228; MAP97; MFI2; MTf; MTF1"
                     /inference="alignment:Splign:2.1.0"
     exon            603..759
                     /gene="MELTF"
                     /gene_synonym="CD228; MAP97; MFI2; MTf; MTF1"
                     /inference="alignment:Splign:2.1.0"
     exon            760..827
                     /gene="MELTF"
                     /gene_synonym="CD228; MAP97; MFI2; MTf; MTF1"
                     /inference="alignment:Splign:2.1.0"
     exon            828..1676
                     /gene="MELTF"
                     /gene_synonym="CD228; MAP97; MFI2; MTf; MTF1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1654..1659
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MELTF"
                     /gene_synonym="CD228; MAP97; MFI2; MTf; MTF1"
                     /note="hexamer: CATAAA"
     polyA_site      1676
                     /gene="MELTF"
                     /gene_synonym="CD228; MAP97; MFI2; MTf; MTF1"
                     /note="major polyA site"
ORIGIN      
        1 gcacttaagg agctcgggcc agcgcgaggg ggagcaggga ggaagcccgg ctgctgcgga
       61 cctcctcgga cccggaccca gccccagccc ggccccagcc agccccgacg gcgccatgcg
      121 gggtccgagc ggggctctgt ggctgctcct ggctctgcgc accgtgctcg gtggcatgga
      181 ggtgcggtgg tgcgccacct cggacccaga gcagcacaag tgcggcaaca tgagcgaggc
      241 cttccgggaa gcgggcatcc agccctccct cctctgcgtc cggggcacct ccgccgacca
      301 ctgcgtccag ctcatcgcgg cccaggaggc tgacgccatc actctggatg gaggagccat
      361 ctatgaggcg ggaaaggagc acggcctgaa gccggtggtg ggcgaagtgt acgatcaaga
      421 ggtcggtacc tcctattacg ccgtggctgt ggtcaggagg agctcccatg tgaccattga
      481 caccctgaaa ggcgtgaagt cctgccacac gggcatcaat cgcacagtgg gctggaacgt
      541 gcccgtgggc tacctggtgg agagcggccg cctctcggtg atgggctgcg atgtactcaa
      601 agctgtcagc gactattttg ggggcagctg cgtcccgggg gcaggagaga ccagttactc
      661 tgagtccctc tgtcgcctct gcaggggtga cagctctggg gaaggggtgt gtgacaagag
      721 ccccctggag agatactacg actacagcgg ggccttccgg tgcctggcgg aaggggcagg
      781 ggacgtggct tttgtgaagc acagcacggt actggagaac acggatgaaa gtccatcacg
      841 aaggcaaaca tggaccagat ctgaggagga agaaggcgag tgccctgcac acgaggaagc
      901 acgtaggacg atgcgctcta gtgctgggca agcctggaaa tgggctcccg ttcacaggcc
      961 ccaggacgag tctgacaaag gagaatttgg aaaacgggca aagagtaggg atatgttggg
     1021 ttaagaatca gctctttcaa acttggggtt ttttttgaga tgggggtctc accatgttgc
     1081 ccaggctggt ctcaaacccc cagcctcaac tgatcctcgc atctcagcct cctgagtagc
     1141 tgggatgaca ggcgtgcacc tggcagcttt ttcaaagtgt tgatggtaat ctgaggcaat
     1201 ctaagggagt cattttttaa gtgactttat acagaaagat tggtaagagc caaggggtag
     1261 aagtggcata aatgtctaaa gcagggaagt gacaggactt tcattgttct tggctgagga
     1321 gaagcgggag tggctgatgg aagcacctaa atgatgcctt tgtctgtggg aaggcaaatg
     1381 atgccccaga gctctaacca aaggttttgc agccgccgaa aaacaggaaa gttgggaagc
     1441 gggggtagga ctacactgaa tcattaacag tgctgtaaac taccatgtgg ccattaacaa
     1501 tgacctttag ggagttttcc taaacgatca ctctggtgcg ggtgtttggt tttgttttaa
     1561 aatagctttg cagtgaaagc tttcatgacc atacaaatta tcttttttct tcctatttcc
     1621 ttgtagaggt ttttttcctc cttgtcttaa ggtcataaaa atattgttat gtggga
//
