LOCUS       NM_001256685            2329 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens maternal embryonic leucine zipper kinase (MELK),
            transcript variant 2, mRNA.
ACCESSION   NM_001256685
VERSION     NM_001256685.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Lee,I.H., Lee,S.J., Kang,B., Lee,J., Jung,J.H., Park,H.Y.,
            Park,J.Y., Park,N.J., Kim,E.A., Kang,J. and Chae,Y.S.
  TITLE     Exploration of MELK as a downstream of Del-1 and druggable targets
            in triple-negative breast cancer
  JOURNAL   Breast Cancer Res Treat 205 (1), 181-191 (2024)
   PUBMED   38279017
  REMARK    GeneRIF: Exploration of MELK as a downstream of Del-1 and druggable
            targets in triple-negative breast cancer.
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Yu,H., Xu,X., Zhu,L., Chen,S. and He,J.
  TITLE     MELK aggravates lung adenocarcinoma by regulating EZH2
            ubiquitination and H3K27me3 histone methylation of LATS2
  JOURNAL   J Cell Mol Med 28 (8), e18216 (2024)
   PUBMED   38652219
  REMARK    GeneRIF: MELK aggravates lung adenocarcinoma by regulating EZH2
            ubiquitination and H3K27me3 histone methylation of LATS2.
REFERENCE   3  (bases 1 to 2329)
  AUTHORS   Lee,D.Y., Lee,S., Kim,Y.S., Park,S., Bae,S.M., Cho,E.A., Park,E.J.,
            Park,H.H., Kim,S.Y., So,I., Chun,J.N. and Jeon,J.H.
  TITLE     Cyclosporin A inhibits prostate cancer growth through suppression
            of E2F8 transcription factor in a MELK-dependent manner
  JOURNAL   Oncol Rep 50 (6) (2023)
   PUBMED   37888771
  REMARK    GeneRIF: Cyclosporin A inhibits prostate cancer growth through
            suppression of E2F8 transcription factor in a MELK-dependent
            manner.
REFERENCE   4  (bases 1 to 2329)
  AUTHORS   Hameed,Y.
  TITLE     Decoding the significant diagnostic and prognostic importance of
            maternal embryonic leucine zipper kinase in human cancers through
            deep integrative analyses
  JOURNAL   J Cancer Res Ther 19 (7), 1852-1864 (2023)
   PUBMED   38376289
  REMARK    GeneRIF: Decoding the significant diagnostic and prognostic
            importance of maternal embryonic leucine zipper kinase in human
            cancers through deep integrative analyses.
REFERENCE   5  (bases 1 to 2329)
  AUTHORS   Xie,X., Chauhan,G.B., Edupuganti,R., Kogawa,T., Park,J., Tacam,M.,
            Tan,A.W., Mughees,M., Vidhu,F., Liu,D.D., Taliaferro,J.M.,
            Pitner,M.K., Browning,L.S., Lee,J.H., Shen,Y., Wang,J., Ueno,N.T.,
            Krishnamurthy,S., Hortobagyi,G.N., Tripathy,D., Van Laere,S.J.,
            Bartholomeusz,G., Dalby,K.N. and Bartholomeusz,C.
  TITLE     Maternal Embryonic Leucine Zipper Kinase is Associated with
            Metastasis in Triple-negative Breast Cancer
  JOURNAL   Cancer Res Commun 3 (6), 1078-1092 (2023)
   PUBMED   37377604
  REMARK    GeneRIF: Maternal Embryonic Leucine Zipper Kinase is Associated
            with Metastasis in Triple-negative Breast Cancer.
            Erratum:[Cancer Res Commun. 2024 Jan 29;4(1):236. doi:
            10.1158/2767-9764.CRC-24-0046.. PMID: 38282551]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2329)
  AUTHORS   Vulsteke,V., Beullens,M., Boudrez,A., Keppens,S., Van Eynde,A.,
            Rider,M.H., Stalmans,W. and Bollen,M.
  TITLE     Inhibition of spliceosome assembly by the cell cycle-regulated
            protein kinase MELK and involvement of splicing factor NIPP1
  JOURNAL   J Biol Chem 279 (10), 8642-8647 (2004)
   PUBMED   14699119
REFERENCE   7  (bases 1 to 2329)
  AUTHORS   Davezac,N., Baldin,V., Blot,J., Ducommun,B. and Tassan,J.P.
  TITLE     Human pEg3 kinase associates with and phosphorylates CDC25B
            phosphatase: a potential role for pEg3 in cell cycle regulation
  JOURNAL   Oncogene 21 (50), 7630-7641 (2002)
   PUBMED   12400006
  REMARK    GeneRIF: pEg3 is a potential regulator of the G2/M progression and
            may act antagonistically to the CDC25B phosphatase,pEg3 kinase is
            able to specifically phosphorylate CDC25B in vitro. One
            phosphorylation site was identified and corresponded to serine 323
REFERENCE   8  (bases 1 to 2329)
  AUTHORS   Seong,H.A., Gil,M., Kim,K.T., Kim,S.J. and Ha,H.
  TITLE     Phosphorylation of a novel zinc-finger-like protein, ZPR9, by
            murine protein serine/threonine kinase 38 (MPK38)
  JOURNAL   Biochem J 361 (Pt 3), 597-604 (2002)
   PUBMED   11802789
REFERENCE   9  (bases 1 to 2329)
  AUTHORS   Gil,M., Yang,Y., Lee,Y., Choi,I. and Ha,H.
  TITLE     Cloning and expression of a cDNA encoding a novel protein
            serine/threonine kinase predominantly expressed in hematopoietic
            cells
  JOURNAL   Gene 195 (2), 295-301 (1997)
   PUBMED   9305775
REFERENCE   10 (bases 1 to 2329)
  AUTHORS   Heyer,B.S., Warsowe,J., Solter,D., Knowles,B.B. and Ackerman,S.L.
  TITLE     New member of the Snf1/AMPK kinase family, Melk, is expressed in
            the mouse egg and preimplantation embryo
  JOURNAL   Mol Reprod Dev 47 (2), 148-156 (1997)
   PUBMED   9136115
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BP365611.1, AK299164.1, BC014039.2 and AI590871.1.
            
            On Aug 13, 2020 this sequence version replaced NM_001256685.1.
            
            Transcript Variant: This variant (2) lacks an exon in the 3' coding
            region, but maintains the reading frame, compared to variant 1. The
            encoded isoform (2) is shorter than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK299164.1, SRR14038195.2794786.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                BP365611.1         36-50
            16-357              AK299164.1         1-342
            358-358             BP365611.1         393-393
            359-1219            AK299164.1         344-1204
            1220-1713           BC014039.2         1330-1823
            1714-1714           AI590871.1         619-619             c
            1715-2329           BC014039.2         1825-2439
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9p13.2"
     gene            1..2329
                     /gene="MELK"
                     /gene_synonym="HPK38"
                     /note="maternal embryonic leucine zipper kinase"
                     /db_xref="GeneID:9833"
                     /db_xref="HGNC:HGNC:16870"
                     /db_xref="MIM:607025"
     exon            1..113
                     /gene="MELK"
                     /gene_synonym="HPK38"
                     /inference="alignment:Splign:2.1.0"
     exon            114..209
                     /gene="MELK"
                     /gene_synonym="HPK38"
                     /inference="alignment:Splign:2.1.0"
     CDS             152..1984
                     /gene="MELK"
                     /gene_synonym="HPK38"
                     /EC_number="2.7.11.1"
                     /EC_number="2.7.10.2"
                     /note="isoform 2 is encoded by transcript variant 2; pEg3
                     kinase; protein kinase PK38; protein kinase Eg3;
                     tyrosine-protein kinase MELK"
                     /codon_start=1
                     /product="maternal embryonic leucine zipper kinase isoform
                     2"
                     /protein_id="NP_001243614.1"
                     /db_xref="CCDS:CCDS59123.1"
                     /db_xref="GeneID:9833"
                     /db_xref="HGNC:HGNC:16870"
                     /db_xref="MIM:607025"
                     /translation="MKDYDELLKYYELHETIGTGGFAKVKLACHILTGEMVAIKIMDK
                     NTLGSDLPRIKTEIEALKNLRHQHICQLYHVLETANKIFMVLEYCPGGELFDYIISQD
                     RLSEEETRVVFRQIVSAVAYVHSQGYAHRDLKPENLLFDEYHKLKLIDFGLCAKPKGN
                     KDYHLQTCCGSLAYAAPELIQGKSYLGSEADVWSMGILLYVLMCGFLPFDDDNVMALY
                     KKIMRGKYDVPKWLSPSSILLLQQMLQVDPKKRISMKNLLNHPWIMQDYNYPVEWQSK
                     NPFIHLDDDCVTELSVHHRNNRQTMEDLISLWQYDHLTATYLLLLAKKARGKPVRLRL
                     SSFSCGQASATPFTDIKFTKYWTESNGVESKSLTPALCRTPANKLKNKENVYTPKSAV
                     KNEEYFMFPEPKTPVNKNQHKREILTTPNRYTTPSKARNQCLKETPIKIPVNSTGTDK
                     LMTGVISPERRCRSVELDLNQAHMEETPKRKGAKVFGSLERGLDKVITVLTRSKRKGS
                     ARDGPRRLKLHYNVTTTRLVNPDQLLNEIMSILPKKHVDFVQKGYTLKCQTQSDFGKV
                     TMQFELEVCQLQKPDVVGIRRQRLKGDAWVYKRLVEDILSSCKV"
     exon            210..295
                     /gene="MELK"
                     /gene_synonym="HPK38"
                     /inference="alignment:Splign:2.1.0"
     exon            296..412
                     /gene="MELK"
                     /gene_synonym="HPK38"
                     /inference="alignment:Splign:2.1.0"
     exon            413..556
                     /gene="MELK"
                     /gene_synonym="HPK38"
                     /inference="alignment:Splign:2.1.0"
     exon            557..625
                     /gene="MELK"
                     /gene_synonym="HPK38"
                     /inference="alignment:Splign:2.1.0"
     exon            626..718
                     /gene="MELK"
                     /gene_synonym="HPK38"
                     /inference="alignment:Splign:2.1.0"
     exon            719..817
                     /gene="MELK"
                     /gene_synonym="HPK38"
                     /inference="alignment:Splign:2.1.0"
     exon            818..886
                     /gene="MELK"
                     /gene_synonym="HPK38"
                     /inference="alignment:Splign:2.1.0"
     exon            887..985
                     /gene="MELK"
                     /gene_synonym="HPK38"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1072
                     /gene="MELK"
                     /gene_synonym="HPK38"
                     /inference="alignment:Splign:2.1.0"
     exon            1073..1204
                     /gene="MELK"
                     /gene_synonym="HPK38"
                     /inference="alignment:Splign:2.1.0"
     exon            1205..1436
                     /gene="MELK"
                     /gene_synonym="HPK38"
                     /inference="alignment:Splign:2.1.0"
     exon            1437..1533
                     /gene="MELK"
                     /gene_synonym="HPK38"
                     /inference="alignment:Splign:2.1.0"
     exon            1534..1702
                     /gene="MELK"
                     /gene_synonym="HPK38"
                     /inference="alignment:Splign:2.1.0"
     exon            1703..1806
                     /gene="MELK"
                     /gene_synonym="HPK38"
                     /inference="alignment:Splign:2.1.0"
     exon            1807..2329
                     /gene="MELK"
                     /gene_synonym="HPK38"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2308..2313
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MELK"
                     /gene_synonym="HPK38"
                     /note="hexamer: AATAAA"
     polyA_site      2329
                     /gene="MELK"
                     /gene_synonym="HPK38"
                     /note="major polyA site"
ORIGIN      
        1 acaacccggc gatcgaaaag attcttagga acgccgtacc agccgcgtct ctcaggacag
       61 caggcccctg tccttctgtc gggcgccgct cagccgtgcc ctccgcccct caggttcttt
      121 ttctaattcc aaataaactt gcaagaggac tatgaaagat tatgatgaac ttctcaaata
      181 ttatgaatta catgaaacta ttgggacagg tggctttgca aaggtcaaac ttgcctgcca
      241 tatccttact ggagagatgg tagctataaa aatcatggat aaaaacacac tagggagtga
      301 tttgccccgg atcaaaacgg agattgaggc cttgaagaac ctgagacatc agcatatatg
      361 tcaactctac catgtgctag agacagccaa caaaatattc atggttcttg agtactgccc
      421 tggaggagag ctgtttgact atataatttc ccaggatcgc ctgtcagaag aggagacccg
      481 ggttgtcttc cgtcagatag tatctgctgt tgcttatgtg cacagccagg gctatgctca
      541 cagggacctc aagccagaaa atttgctgtt tgatgaatat cataaattaa agctgattga
      601 ctttggtctc tgtgcaaaac ccaagggtaa caaggattac catctacaga catgctgtgg
      661 gagtctggct tatgcagcac ctgagttaat acaaggcaaa tcatatcttg gatcagaggc
      721 agatgtttgg agcatgggca tactgttata tgttcttatg tgtggatttc taccatttga
      781 tgatgataat gtaatggctt tatacaagaa gattatgaga ggaaaatatg atgttcccaa
      841 gtggctctct cccagtagca ttctgcttct tcaacaaatg ctgcaggtgg acccaaagaa
      901 acggatttct atgaaaaatc tattgaacca tccctggatc atgcaagatt acaactatcc
      961 tgttgagtgg caaagcaaga atccttttat tcacctcgat gatgattgcg taacagaact
     1021 ttctgtacat cacagaaaca acaggcaaac aatggaggat ttaatttcac tgtggcagta
     1081 tgatcacctc acggctacct atcttctgct tctagccaag aaggctcggg gaaaaccagt
     1141 tcgtttaagg ctttcttctt tctcctgtgg acaagccagt gctaccccat tcacagacat
     1201 caagtttacc aagtactgga cagaatcaaa tggggtggaa tctaaatcat taactccagc
     1261 cttatgcaga acacctgcaa ataaattaaa gaacaaagaa aatgtatata ctcctaagtc
     1321 tgctgtaaag aatgaagagt actttatgtt tcctgagcca aagactccag ttaataagaa
     1381 ccagcataag agagaaatac tcactacgcc aaatcgttac actacaccct caaaagctag
     1441 aaaccagtgc ctgaaagaaa ctccaattaa aataccagta aattcaacag gaacagacaa
     1501 gttaatgaca ggtgtcatta gccctgagag gcggtgccgc tcagtggaat tggatctcaa
     1561 ccaagcacat atggaggaga ctccaaaaag aaagggagcc aaagtgtttg ggagccttga
     1621 aagggggttg gataaggtta tcactgtgct caccaggagc aaaaggaagg gttctgccag
     1681 agacgggccc agaagactaa agcttcacta taacgtgact acaactagat tagtgaatcc
     1741 agatcaactg ttgaatgaaa taatgtctat tcttccaaag aagcatgttg actttgtaca
     1801 aaagggttat acactgaagt gtcaaacaca gtcagatttt gggaaagtga caatgcaatt
     1861 tgaattagaa gtgtgccagc ttcaaaaacc cgatgtggtg ggtatcagga ggcagcggct
     1921 taagggcgat gcctgggttt acaaaagatt agtggaagac atcctatcta gctgcaaggt
     1981 ataattgatg gattcttcca tcctgccgga tgagtgtggg tgtgatacag cctacataaa
     2041 gactgttatg atcgctttga ttttaaagtt cattggaact accaacttgt ttctaaagag
     2101 ctatcttaag accaatatct ctttgttttt aaacaaaaga tattattttg tgtatgaatc
     2161 taaatcaagc ccatctgtca ttatgttact gtctttttta atcatgtggt tttgtatatt
     2221 aataattgtt gactttctta gattcacttc catatgtgaa tgtaagctct taactatgtc
     2281 tctttgtaat gtgtaatttc tttctgaaat aaaaccattt gtgaatata
//
