LOCUS       NM_001271938           11137 bp    mRNA    linear   PRI 01-AUG-2024
DEFINITION  Homo sapiens multiple EGF like domains 8 (MEGF8), transcript
            variant 1, mRNA.
ACCESSION   NM_001271938
VERSION     NM_001271938.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11137)
  AUTHORS   Watts,L.M., Bertoli,M., Attie-Bitach,T., Roux,N., Rausell,A.,
            Paschal,C.R., Zambonin,J.L., Curry,C.J., Martin,B., Tooze,R.S.,
            Hawkes,L., Kini,U., Twigg,S.R.F. and Wilkie,A.O.M.
  TITLE     The phenotype of MEGF8-related Carpenter syndrome (CRPT2) is
            refined through the identification of eight new patients
  JOURNAL   Eur J Hum Genet 32 (7), 864-870 (2024)
   PUBMED   38760421
  REMARK    GeneRIF: The phenotype of MEGF8-related Carpenter syndrome (CRPT2)
            is refined through the identification of eight new patients.
REFERENCE   2  (bases 1 to 11137)
  AUTHORS   Yuan,X., Chen,R., Luo,G., Sun,P., Song,X., Ma,J., Sun,R., Yu,T. and
            Jiang,Z.
  TITLE     Role and mechanism of miR-871-3p/Megf8 in regulating
            formaldehyde-induced cardiomyocyte inflammation and congenital
            heart disease
  JOURNAL   Int Immunopharmacol 126, 111297 (2024)
   PUBMED   38039718
  REMARK    GeneRIF: Role and mechanism of miR-871-3p/Megf8 in regulating
            formaldehyde-induced cardiomyocyte inflammation and congenital
            heart disease.
REFERENCE   3  (bases 1 to 11137)
  AUTHORS   Twigg,S.R., Lloyd,D., Jenkins,D., Elcioglu,N.E., Cooper,C.D.,
            Al-Sannaa,N., Annagur,A., Gillessen-Kaesbach,G., Huning,I.,
            Knight,S.J., Goodship,J.A., Keavney,B.D., Beales,P.L., Gileadi,O.,
            McGowan,S.J. and Wilkie,A.O.
  TITLE     Mutations in multidomain protein MEGF8 identify a Carpenter
            syndrome subtype associated with defective lateralization
  JOURNAL   Am J Hum Genet 91 (5), 897-905 (2012)
   PUBMED   23063620
  REMARK    GeneRIF: mutations in MEGF8 cause a Carpenter syndrome subtype
            frequently associated with defective left-right patterning,
            probably through perturbation of signaling by hedgehog and nodal
            family members.
REFERENCE   4  (bases 1 to 11137)
  AUTHORS   Kahle,J.J., Gulbahce,N., Shaw,C.A., Lim,J., Hill,D.E.,
            Barabasi,A.L. and Zoghbi,H.Y.
  TITLE     Comparison of an expanded ataxia interactome with patient medical
            records reveals a relationship between macular degeneration and
            ataxia
  JOURNAL   Hum Mol Genet 20 (3), 510-527 (2011)
   PUBMED   21078624
REFERENCE   5  (bases 1 to 11137)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   6  (bases 1 to 11137)
  AUTHORS   Nakayama,M., Nakajima,D., Nagase,T., Nomura,N., Seki,N. and
            Ohara,O.
  TITLE     Identification of high-molecular-weight proteins with multiple
            EGF-like motifs by motif-trap screening
  JOURNAL   Genomics 51 (1), 27-34 (1998)
   PUBMED   9693030
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC153880.1, AY280362.1 and
            AC011497.6.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_001271938.1.
            
            Summary: The protein encoded by this gene is a single-pass type I
            membrane protein of unknown function that contains several EGF-like
            domains, Kelch repeats, and PSI domains. Defects in this gene are a
            cause of Carpenter syndrome 2. Two transcript variants encoding
            different isoforms have been found for this gene. [provided by
            RefSeq, Dec 2012].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA1968832,
                              SAMEA1968968 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000251268.11/ ENSP00000251268.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1853              BC153880.1         27-1879
            1854-9833           AY280362.1         2-7981
            9834-10174          BC153880.1         9659-9999
            10175-11137         AC011497.6         120695-121657       c
FEATURES             Location/Qualifiers
     source          1..11137
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.2"
     gene            1..11137
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /note="multiple EGF like domains 8"
                     /db_xref="GeneID:1954"
                     /db_xref="HGNC:HGNC:3233"
                     /db_xref="MIM:604267"
     exon            1..796
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             610..9147
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; EGF-like-domain, multiple 4; EGF-like
                     domain-containing protein 4; multiple epidermal growth
                     factor-like domains protein 8; HBV pre-s2 binding protein
                     1; HBV pre-S2-binding protein 1; hepatitis B virus
                     pre-S2-binding protein 1; epidermal growth factor-like
                     protein 4"
                     /codon_start=1
                     /product="multiple epidermal growth factor-like domains
                     protein 8 isoform 1 precursor"
                     /protein_id="NP_001258867.1"
                     /db_xref="CCDS:CCDS62693.1"
                     /db_xref="GeneID:1954"
                     /db_xref="HGNC:HGNC:3233"
                     /db_xref="MIM:604267"
                     /translation="MALGKVLAMALVLALAVLGSLSPGARAGDCKGQRQVLREAPGFV
                     TDGAGNYSVNGNCEWLIEAPSPQHRILLDFLFLDTECTYDYLFVYDGDSPRGPLLASL
                     SGSTRPPPIEASSGKMLLHLFSDANYNLLGFNASFRFSLCPGGCQSHGQCQPPGVCAC
                     EPGWGGPDCGLQECSAYCGSHGTCASPLGPCRCEPGFLGRACDLHLWENQGAGWWHNV
                     SARDPAFSARIGAAGAFLSPPGLLAVFGGQDLNNALGDLVLYNFSANTWESWDLSPAP
                     AARHSHVAVAWAGSLVLMGGELADGSLTNDVWAFSPLGRGHWELLAPPASSSSGPPGL
                     AGHAAALVDDVWLYVSGGRTPHDLFSSGLFRFRLDSTSGGYWEQVIPAGGRPPAATGH
                     SMVFHAPSRALLVHGGHRPSTARFSVRVNSTELFHVDRHVWTTLKGRDGLQGPRERAF
                     HTASVLGNYMVVYGGNVHTHYQEEKCYEDGIFFYHLGCHQWVSGAELAPPGTPEGRAA
                     PPSGRYSHVAAVLGGSVLLVAGGYSGRPRGDLMAYKVPPFVFQAPAPDYHLDYCSMYT
                     DHSVCSRDPECSWCQGACQAAPPPGTPLGACPAASCLGLGRLLGDCQACLAFSSPTAP
                     PRGPGTLGWCVHNESCLPRPEQARCRGEQISGTVGWWGPAPVFVTSLEACVTQSFLPG
                     LHLLTFQQPPNTSQPDKVSIVRSTTITLTPSAETDVSLVYRGFIYPMLPGGPGGPGAE
                     DVAVWTRAQRLHVLARMARGPDTENMEEVGRWVAHQEKETRRLQRPGSARLFPLPGRD
                     HKYAVEIQGQLNGSAGPGHSELTLLWDRTGVPGGSEISFFFLEPYRSSSCTSYSSCLG
                     CLADQGCGWCLTSATCHLRQGGAHCGDDGAGGSLLVLVPTLCPLCEEHRDCHACTQDP
                     FCEWHQSTSRKGDAACSRRGRGRGALKSPEECPPLCSQRLTCEDCLANSSQCAWCQST
                     HTCFLFAAYLARYPHGGCRGWDDSVHSEPRCRSCDGFLTCHECLQSHECGWCGNEDNP
                     TLGRCLQGDFSGPLGGGNCSLWVGEGLGLPVALPARWAYARCPDVDECRLGLARCHPR
                     ATCLNTPLSYECHCQRGYQGDGISHCNRTCLEDCGHGVCSGPPDFTCVCDLGWTSDLP
                     PPTPAPGPPAPRCSRDCGCSFHSHCRKRGPGFCDECQDWTWGEHCERCRPGSFGNATG
                     SRGCRPCQCNGHGDPRRGHCDNLSGLCFCQDHTEGAHCQLCSPGYYGDPRAGGSCFRE
                     CGGRALLTNVSSVALGSRRVGGLLPPGGGAARAGPGLSYCVWVVSATEELQPCAPGTL
                     CPPLTLTFSPDSSTPCTLSYVLAFDGFPRFLDTGVVQSDRSLIAAFCGQRRDRPLTVQ
                     ALSGLLVLHWEANGSSSWGFNASVGSARCGSGGPGSCPVPQECVPQDGAAGAGLCRCP
                     QGWAGPHCRMALCPENCNAHTGAGTCNQSLGVCICAEGFGGPDCATKLDGGQLVWETL
                     MDSRLSADTASRFLHRLGHTMVDGPDATLWMFGGLGLPQGLLGNLYRYSVSERRWTQM
                     LAGAEDGGPGPSPRSFHAAAYVPAGRGAMYLLGGLTAGGVTRDFWVLNLTTLQWRQEK
                     APQTVELPAVAGHTLTARRGLSLLLVGGYSPENGFNQQLLEYQLATGTWVSGAQSGTP
                     PTGLYGHSAVYHEATDSLYVFGGFRFHVELAAPSPELYSLHCPDRTWSLLAPSQGAKR
                     DRMRNVRGSSRGLGQVPGEQPGSWGFREVRKKMALWAALAGTGGFLEEISPHLKEPRP
                     RLFHASALLGDTMVVLGGRSDPDEFSSDVLLYQVNCNAWLLPDLTRSASVGPPMEESV
                     AHAVAAVGSRLYISGGFGGVALGRLLALTLPPDPCRLLSSPEACNQSGACTWCHGACL
                     SGDQAHRLGCGGSPCSPMPRSPEECRRLRTCSECLARHPRTLQPGDGEASTPRCKWCT
                     NCPEGACIGRNGSCTSENDCRINQREVFWAGNCSEAACGAADCEQCTREGKCMWTRQF
                     KRTGETRRILSVQPTYDWTCFSHSLLNVSPMPVESSPPLPCPTPCHLLPNCTSCLDSK
                     GADGGWQHCVWSSSLQQCLSPSYLPLRCMAGGCGRLLRGPESCSLGCAQATQCALCLR
                     RPHCGWCAWGGQDGGGRCMEGGLSGPRDGLTCGRPGASWAFLSCPPEDECANGHHDCN
                     ETQNCHDQPHGYECSCKTGYTMDNMTGLCRPVCAQGCVNGSCVEPDHCRCHFGFVGRN
                     CSTECRCNRHSECAGVGARDHCLLCRNHTKGSHCEQCLPLFVGSAVGGGTCRPCHAFC
                     RGNSHICISRKELQMSKGEPKKYSLDPEEIENWVTEGPSEDEAVCVNCQNNSYGEKCE
                     SCLQGYFLLDGKCTKCQCNGHADTCNEQDGTGCPCQNNTETGTCQGSSPSDRRDCYKY
                     QCAKCRESFHGSPLGGQQCYRLISVEQECCLDPTSQTNCFHEPKRRALGPGRTVLFGV
                     QPKFTNVDIRLTLDVTFGAVDLYVSTSYDTFVVRVAPDTGVHTVHIQPPPAPPPPPPP
                     ADGGPRGAGDPGGAGASSGPGAPAEPRVREVWPRGLITYVTVTEPSAVLVVRGVRDRL
                     VITYPHEHHALKSSRFYLLLLGVGDPSGPGANGSADSQGLLFFRQDQAHIDLFVFFSV
                     FFSCFFLFLSLCVLLWKAKQALDQRQEQRRHLQEMTKMASRPFAKVTVCFPPDPTAPA
                     SAWKPAGLPPPAFRRSEPFLAPLLLTGAGGPWGPMGGGCCPPAIPATTAGLRAGPITL
                     EPTEDGMAGVATLLLQLPGGPHAPNGACLGSALVTLRHRLHEYCGGGGGAGGSGHGTG
                     AGRKGLLSQDNLTSMSL"
     exon            797..960
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            961..1167
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1168..1348
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1349..1437
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1438..1853
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1854..1999
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2000..2122
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2123..2277
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2278..2397
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2398..2542
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2543..2706
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2707..2907
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2908..3108
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3109..3345
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3346..3464
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3465..3596
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3597..3710
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3711..3959
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3960..4159
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            4160..4370
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            4371..4620
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            4621..4753
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            4754..5001
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            5002..5112
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            5113..5231
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            5232..5439
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            5440..5620
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            5621..5784
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            5785..5952
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            5953..6097
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            6098..6329
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            6330..6453
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            6454..6667
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            6668..6882
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            6883..7090
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            7091..7250
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            7251..7443
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            7444..7614
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            7615..7745
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            7746..7878
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     exon            7879..11137
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      11118..11123
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /note="hexamer: AATAAA"
     polyA_site      11137
                     /gene="MEGF8"
                     /gene_synonym="C19orf49; CRPT2; EGFL4; SBP1"
                     /note="major polyA site"
ORIGIN      
        1 gtctctctga tctggcccat ccggcttcgg agggaggcga gggtgggtga gcaaagggat
       61 tgggtctgtg gggtccaggc ccgaacccct gaagacgggc tccgcccccg gcacccgctc
      121 gcgccccgcc cccggctgga ggagtctctc ctggaccatc cgaacctagc ctgtcccggc
      181 ccgcagcctc tatggaggct cctgccgggc cgtagagccc ttcgccccct ggggacccac
      241 ccgtctataa ggtccgtttg gcctgcagca gcctgagtcc gtaatgctgg gcactgttca
      301 tgggatcggc cccctatgga gccctgtgtc tataggggac tcctacggtc cctagggttc
      361 ggccccgtcc ataatgactc catatacagg gccttccatc gctctatagg gctcagccct
      421 cggcttccag agcctgtcag cagtggccgt acccttcgcc gggactgccg ggtctccggg
      481 acctcttcga tctacaaggt catgttatgc ctatagaggt cgcatttgca gggcctcacc
      541 ccgggtagag ggtcctctcc aggtttttac ggcctgtccc cgctctaagg gtcagtgcag
      601 gaggcggcga tggccctggg caaggttctg gccatggcac tggttttggc cttggccgtg
      661 ctggggtcgc tgtcccctgg ggcccgggcg ggggactgca aggggcagcg gcaggtgctg
      721 cgggaggcgc caggcttcgt gacggatggt gcgggcaact acagcgtcaa tggcaactgc
      781 gagtggctca tcgaggcccc aagcccccag caccggatcc tgctggactt ccttttcctg
      841 gacacagagt gcacgtatga ctacctgttc gtgtatgacg gtgactcccc gcgagggccg
      901 ctgcttgcca gtctaagtgg gagcacccga cctccgccca tcgaagcttc ctcaggcaag
      961 atgctgctgc acctcttcag tgatgccaac tacaacctgc tgggctttaa cgcctcattc
     1021 cgcttctccc tgtgcccggg tggctgccag agccacgggc agtgccagcc accgggtgtg
     1081 tgtgcctgcg agccgggctg ggggggtcct gactgtggcc tgcaggagtg ctcagcctac
     1141 tgtggcagcc acggcacctg cgcctcgccc ctgggaccat gccgctgtga gcctggcttc
     1201 ttgggacgtg cctgtgacct gcacctgtgg gagaaccagg gggctgggtg gtggcacaac
     1261 gtgagtgcca gggaccctgc cttctctgcc cgtattgggg cagctggcgc cttcctgtcc
     1321 ccaccagggc tgctggcagt tttcggaggc caggacctca acaatgccct gggtgacctc
     1381 gtcctataca acttctccgc caacacctgg gagtcttggg acctgagtcc tgccccggct
     1441 gcccgtcact cccatgtggc cgtggcctgg gccggctccc tggtactgat gggtggtgag
     1501 ctggctgacg gctcgctcac caacgacgtg tgggccttca gtccactggg caggggccac
     1561 tgggagctcc tggcaccacc tgcctccagc tcctcggggc ccccaggcct ggcaggtcac
     1621 gcggctgccc tggtggatga tgtctggcta tatgtgtctg gaggccgcac cccgcacgac
     1681 ctcttctcct ctggcctctt ccgtttccgc cttgacagca ccagcggggg ctattgggag
     1741 caggtgattc cggcaggcgg acggccccct gctgccactg gccactccat ggtgttccat
     1801 gccccctccc gtgccctgct ggtccatggt ggacaccggc cctccactgc ccggttctct
     1861 gtgcgagtga actccactga gcttttccac gtggatcggc atgtgtggac gacgctgaag
     1921 gggcgggatg ggcttcaggg cccaagggag cgagccttcc acacagccag tgttctgggc
     1981 aattacatgg tggtctatgg gggcaatgtg cacacccatt accaggagga aaagtgctac
     2041 gaagatggca tcttcttcta ccaccttggc tgccatcaat gggtgtcagg agctgagctt
     2101 gccccgccag gaacccctga gggccgagca gcgcctccca gtggtcggta ctcacatgta
     2161 gctgcggtgc ttggtggcag cgtcctgttg gtggctgggg ggtacagcgg ccggccccgt
     2221 ggggacttga tggcgtacaa ggtgcccccc tttgtgttcc aggcacctgc ccctgactac
     2281 cacttggact actgctccat gtacacagac cacagcgtct gctcccggga cccggaatgc
     2341 agttggtgcc aaggagcctg ccaagctgca ccccctcctg ggaccccctt gggggcttgt
     2401 ccagccgcca gctgcctggg cctgggccgc ctcctgggtg actgccaggc ctgcctggcc
     2461 ttcagcagcc ccacagcccc tccacgggga cctggcaccc tgggctggtg cgtgcacaat
     2521 gagagctgcc tccctaggcc tgagcaggcc cgctgccgag gggagcagat ctcaggcact
     2581 gtgggctggt gggggcctgc gcctgtcttc gtcacgtccc tggaggcctg cgtcacccag
     2641 agcttcctgc ctggcctgca cttgctcacc tttcagcagc cgcccaatac ctcccagcct
     2701 gacaaggtct caattgtccg cagcacgacc atcaccctaa cacccagcgc agagacagat
     2761 gtgtccctgg tctaccgtgg cttcatctac ccaatgctgc ctggagggcc aggtggacca
     2821 ggggctgagg acgtggccgt gtggacgcgg gcccagcgcc tacacgtcct ggcccggatg
     2881 gcccgtggcc ctgacacgga gaacatggag gaggtggggc gctgggtggc tcatcaggag
     2941 aaggagacgc ggcggctgca gcgccctggg tctgctcgcc tcttccctct gcctgggcgg
     3001 gaccacaagt atgcagtaga gatccagggc cagctcaatg gctcggcagg ccctgggcac
     3061 agcgagctaa ctctgctgtg ggatcggact ggtgtgccag gaggcagcga gatctccttc
     3121 ttcttcctgg agccctaccg ctcgtcgtcc tgcacctcct attcttcctg cctgggctgc
     3181 ttggcagacc agggctgtgg ctggtgcctg accagtgcca cctgccacct gcgccagggc
     3241 ggagcccatt gcggggatga cggggctggt gggtccctgc tggtgctggt gcctaccctc
     3301 tgcccactct gcgaggagca tcgggactgc cacgcctgca cccaggaccc cttctgtgag
     3361 tggcatcaga gcaccagccg caaaggggac gcggcatgca gccggcgggg ccggggtcgg
     3421 ggtgccctga agagtccaga ggagtgtccc ccgctctgca gccagcgact gacctgtgag
     3481 gactgcctgg ccaactctag ccagtgcgcc tggtgccagt ccacccacac ctgcttcctg
     3541 tttgctgcct acttggcccg gtacccacac gggggctgtc gaggctggga cgacagtgta
     3601 cactcggagc cacggtgccg gagctgcgat ggcttcctga cctgccatga gtgtctgcag
     3661 agccacgagt gtggctggtg tggcaatgag gacaacccca cactgggacg gtgcctacag
     3721 ggggacttct cagggcccct cggtgggggt aactgctccc tgtgggtggg ggagggcctg
     3781 gggcttcccg tggccctccc tgcccgctgg gcatacgccc gctgtcctga cgtggatgag
     3841 tgtcgcctgg gcctggcccg gtgccacccg cgggcgacct gcctgaacac gcccctcagc
     3901 tacgagtgtc actgccagcg gggctaccag ggtgatggca tctcacactg caaccgcacg
     3961 tgcttggagg actgtggcca tggtgtgtgc agtggccccc cggactttac ctgcgtgtgt
     4021 gacctaggct ggacatcaga cctgccccct cccacacccg ccccgggtcc gccagccccc
     4081 cgctgctccc gggactgtgg ctgcagcttc cacagccact gccgcaagcg gggccctggc
     4141 ttctgcgacg agtgccagga ctggacatgg ggggagcact gcgaacgatg ccggcccggc
     4201 agcttcggca acgccacagg ctctaggggc tgccggccct gccagtgcaa cgggcacggg
     4261 gacccacgcc gtggccactg cgacaacctc agtgggctct gcttctgcca ggaccacacc
     4321 gagggtgccc actgccagct ctgctcccca ggctattatg gggatccccg ggccggtggt
     4381 tcctgctttc gggagtgtgg gggtcgcgcc ctcctcacca acgtgtcctc agtggcactg
     4441 ggctcacgcc gggtcggggg gctgctgcct ccaggtggcg gggctgcaag agccgggcct
     4501 ggcctgtcct actgtgtgtg ggttgtctcg gccactgagg agctacagcc ctgtgctccc
     4561 gggaccctct gtcccccact caccctcacc ttctcccccg acagcagcac cccctgcacg
     4621 ctgagctacg tcctggcgtt tgatggattc ccacgcttcc tggacactgg tgttgtccag
     4681 tcggaccgca gcctcatagc tgccttctgc ggccagcgac gggacaggcc cctcactgtt
     4741 caggccctgt ctgggctgct cgtgctgcac tgggaggcca atggctcctc atcctggggc
     4801 ttcaatgctt cggtgggctc tgcccgctgt gggtcagggg gccccgggag ctgtcccgtc
     4861 ccccaggaat gcgtgcccca ggacggtgct gcaggtgcgg ggctctgccg atgtcctcag
     4921 ggctgggctg gcccacactg ccgcatggct ctgtgtcctg agaactgcaa tgcccacact
     4981 ggggcaggaa cttgtaacca gagcctgggt gtgtgcatct gtgccgaggg cttcgggggc
     5041 cccgactgcg ccaccaagct ggatggcggg cagctggtct gggagaccct catggacagc
     5101 cgcctctcag ccgacactgc cagccgcttc ctgcaccgcc tgggccacac catggtggat
     5161 ggacccgatg ccaccttgtg gatgtttggg ggcctgggcc tgccccaggg gctgctggga
     5221 aacctgtaca ggtactcagt gagtgagcgg cggtggacac agatgctggc gggagccgag
     5281 gacgggggcc caggcccatc gccccgctcc ttccatgcag ccgcatatgt gcccgctggc
     5341 cgtggtgcca tgtatctgct ggggggactt accgctggag gcgtcacccg tgatttctgg
     5401 gtcctcaacc tcaccaccct gcaatggcgg caggagaagg ccccccagac cgtggagctg
     5461 ccagccgttg ctggtcacac ccttactgcc cgccgaggcc tgtctctgct cctggtgggc
     5521 ggttactccc cggaaaatgg cttcaaccag cagctgctgg agtaccagct ggcaaccggc
     5581 acctgggtgt caggagccca gagtgggaca ccccccacag gtctctatgg tcactctgct
     5641 gtctaccacg aggccaccga ctccctctac gtgtttgggg ggttccgatt ccatgtggag
     5701 ctggcggccc catcccccga gctctactcc ctgcactgtc ctgaccgcac ctggagtctg
     5761 ctggcccctt ctcagggggc aaagcgagat cgtatgagga atgtgcgtgg ctcatctcgg
     5821 ggtctgggcc aagttcctgg ggagcagcct gggtcatggg ggttccggga agtcaggaag
     5881 aagatggctc tgtgggctgc tcttgctggt acaggaggtt tcctggagga aatctcacct
     5941 cacctgaagg agccccgccc ccggcttttc cacgcctcag ccctgttagg ggacaccatg
     6001 gtggttcttg gggggcgctc ggaccctgac gagttcagca gcgacgttct gctctaccag
     6061 gtcaactgca atgcctggct tctgcccgac ctcacccgct cggcctctgt ggggccccca
     6121 atggaggagt ctgtggccca tgctgtggca gcagtcggga gccgcctgta tatctctggg
     6181 ggtttcgggg gagtggccct gggccgcctg ctggcactga ccctgccccc tgacccctgc
     6241 cgcctgctgt cctcacctga agcttgtaac cagtctgggg cctgcacctg gtgccatggg
     6301 gcctgcttgt ccggggatca ggcccacagg ctgggctgcg ggggctcccc ctgctcccca
     6361 atgcctcgct ccccggagga atgtcgacgt ctccggacct gcagtgagtg cctggcccgc
     6421 catcctcgga ccctgcaacc tggagatgga gaggcgtcca ccccccgctg taagtggtgt
     6481 accaactgcc ccgaaggtgc ttgcattgga cgcaatgggt cctgcacctc tgagaatgac
     6541 tgtcggatca accagcgaga ggtcttctgg gcagggaact gctccgaggc tgcgtgcggg
     6601 gctgctgact gcgagcagtg cacgcgggag ggcaagtgca tgtggacgcg gcagttcaag
     6661 aggacagggg agacccgccg catcctctcc gtgcagccca cctatgactg gacgtgcttc
     6721 agccactctc tgctgaatgt gtcccccatg ccggtggaat catcaccccc actgccctgc
     6781 cccacccctt gtcacctcct acccaactgt acctcctgcc tggactctaa gggagcagat
     6841 gggggctggc agcactgtgt ttggagcagc agcctgcagc agtgtctgag cccttcctac
     6901 ctgcccctgc gatgtatggc cggaggctgt gggcggctgc tccggggacc tgagagctgc
     6961 tccctgggct gtgctcaggc aactcagtgc gccttgtgcc tgcggcgccc ccattgcggc
     7021 tggtgtgcct gggggggcca ggatgggggt ggccgctgca tggagggtgg actcagcggc
     7081 ccccgtgatg ggctgacatg tgggcgtccg ggggcctcct gggccttcct gtcctgcccc
     7141 cctgaggacg agtgtgcaaa cgggcaccac gactgcaacg agacgcagaa ttgccacgac
     7201 cagccccacg gctatgagtg cagctgcaag accggctata ccatggacaa catgacaggg
     7261 ctgtgccgcc ctgtgtgcgc ccagggctgc gtgaacggct catgtgtgga gcccgaccac
     7321 tgccgctgcc actttggctt tgtgggccgc aactgctcca cggaatgccg ctgcaaccgc
     7381 cacagtgaat gcgctggtgt tggggcgcgt gaccactgct tgctctgccg caaccacacc
     7441 aagggcagcc actgtgagca gtgcctcccg ctgtttgtgg gttcagctgt cggaggcggg
     7501 acctgccggc cctgccacgc cttttgtcgt ggaaatagcc acatctgcat ctccaggaag
     7561 gagttacaaa tgtccaaggg agagccaaag aagtactcac tggacccaga ggagattgaa
     7621 aactgggtga cagagggtcc tagtgaagac gaggccgtgt gcgtgaactg ccagaataac
     7681 agctatgggg agaaatgcga gagctgcctg cagggctact tcctcctgga cgggaagtgc
     7741 accaaatgcc agtgtaatgg ccacgcggac acatgtaacg agcaggatgg gacgggctgt
     7801 ccatgtcaga ataacacaga gacgggcaca tgccagggca gctcccccag tgaccgtcga
     7861 gactgctaca agtaccagtg cgccaagtgc cgggaatcat ttcacgggag tccgctgggc
     7921 ggccagcagt gctaccgcct catctcggtg gagcaggagt gctgcctgga ccccacgtcc
     7981 cagaccaact gcttccatga gcccaaacgc cgggcgctag gccccggccg cactgtcctc
     8041 tttggcgtgc agcccaaatt caccaacgtg gacatccgcc tgacgctgga cgtgaccttc
     8101 ggggccgtgg acctctatgt ctccacctcc tatgacacct tcgtggtccg tgtggcccct
     8161 gacactggcg tccatactgt acacatccag ccacccccag ccccaccacc tccaccaccc
     8221 cctgcagatg gtgggccccg gggggctggg gatccaggag gagcaggggc cagcagtggg
     8281 ccgggcgccc cagcagagcc acgggtacgg gaggtatggc cgcggggcct gattacctac
     8341 gtgacggtga cggagccgtc ggcagtgctg gtggtccgcg gcgtgcggga ccggctggtc
     8401 atcacctacc cacacgagca ccatgccctc aagtcgagcc gcttctacct gctgctgctg
     8461 ggcgtgggag acccaagtgg gcccggcgcc aacggctcag ccgactcgca gggcctgctc
     8521 ttcttccggc aggaccaggc ccacattgac ctgtttgtct tcttctccgt cttcttctcc
     8581 tgcttcttcc tcttcctctc actctgtgtg ctcctctgga aggccaagca ggctctggac
     8641 cagcggcagg agcagcgccg gcacttgcag gagatgacca agatggccag ccgccccttc
     8701 gccaaggtca ccgtctgctt cccacctgac cctactgccc cggcctccgc ctggaagccg
     8761 gctgggctcc cacctcccgc cttccgccgc tctgagccct tcctggcacc cctgctgctg
     8821 acaggggccg gtgggccctg gggacccatg ggagggggct gctgcccacc agccatcccc
     8881 gccaccactg ctgggctgcg agctgggccc atcactctcg agcccacaga agatggcatg
     8941 gctggcgtgg ccacactgct gctccagctg cctggcgggc cccatgcacc caacggcgcc
     9001 tgcctggggt cagccctcgt cacactgcgg cacaggctgc acgagtactg tgggggtggt
     9061 gggggtgctg ggggcagtgg gcatgggact ggtgcgggcc ggaagggact gttgagccag
     9121 gacaacctca ccagcatgtc cctctgacat gcccagggtt ctcatccaca gcagctgggt
     9181 cacctgatag ggccgccctg gacttggggt ccctccacct gggggcccct ggacactgtc
     9241 tacttggaga ccactggccc ccttccccca gggttgccca gatggggcct cctttgttct
     9301 gcattcagca gctatttatc gagtacctac tctgtcaggc actgtcatag gcgtggggca
     9361 aagcaggaac caagagacga ggttccctga tctcatggga cttaggttct ggtgaaggga
     9421 gacaatcagt gcacatgcac acaccccaca cgcatacaca catgaacaca tgcacatgtg
     9481 cacacacaag taagatggct tcagagaggg agaagcactg tgaggcctcc agaggatgtg
     9541 gcagtgaggg acgatggggt gaagtcagct gggcattcaa agaagctaga ctgagaacgc
     9601 ctgagaagaa ccagctacgg gaagagcttt gggaagcaaa ggcagaggcc ctggggtggg
     9661 agcaggcttg ttttattgga aggaccagaa aactggtaag tgtgacccag atcaagtgtg
     9721 aggagatgag gctggggata gtcaggggct ggatcaccca gggccttgtg ggccccacat
     9781 agggttttgg gttttattct cagggcaatg ggaagctgtt ggatggtttg atgaagggga
     9841 gtgacaggat ccgatgtacc tatttaagaa tttaagaggg tcgggtgcgg tggctcatgc
     9901 ctataatcct agcactttga gaggccaagg tgggctgatc acaaggtcaa gagttcgtga
     9961 ccagcctggc caatatggtg aaaccccatg tctactaaaa atacaaaaat tagctgggca
    10021 tggtggcaca cacttgtagt ccgagctact cgggaggctg aggcagaaga atcgcttgaa
    10081 cccaggaggc ggaggttgca gtgagctcag attgcgtcac tgcactccaa cctgggtgac
    10141 agagcgagac tccacctcaa aaaaaaaaaa aaaatttaag aggtcactca gttgtgctgt
    10201 ggagaatgga ccggagggac aagaggggca gcagggatgg tgggctggag tagggtgctg
    10261 gcaatgaggg agtctggctc agatgtggga tgtgtatgga agaatataaa tgatggtgtg
    10321 gatgtcaggg tgagggagga gacaaaacca cgatgacccc tagctttgtg gcctgaactg
    10381 tgggtggctg aggggatcgt taattgaatg gggcagactg aggcttgtga ggaagatcag
    10441 agtctggttc ttgacatgag atgcccttca gacatctctt cactcaggtc caactaggga
    10501 tacagaaaca ctgaatattt caacagcaga aattgaatgg ggggattgat agcgctggcg
    10561 agggaagcag ctggaaagag acagatggca ccctgagaca gcccagaggt gaataggacc
    10621 cccaggctgc agggataaag ctcagtggtg gtgttacctc accggggacc agggtcacac
    10681 agcaaagctg gaacaacaga ggcgtgttgt gggggagcct cagaggggac aaaacctctg
    10741 cctgagatcc caccccaggt gggcatgggg gccactgagg ttggggatga aaatgccggt
    10801 accgtcagtg cacagccctg ttccagacag tgctgcctgg aagatttctg ggctctcctg
    10861 aggcgccacc ccgcacctga gccacctcct tggactcctg tcctctaccc cttgaggacc
    10921 tccctccctt ctaccctagc tgtcttcttg aacttgggac tctcctttcc caagacttcc
    10981 atcactagct cctggaggga ctggactttg catcttccct tcgcgtggag cctcagtgtg
    11041 agaggcccta gccaatgcgt gcatgtcaga ggtggtgggg accacatcag aagaagaggg
    11101 gggtgatgaa attaacaaat aaaaagtatg gggaaaa
//
