LOCUS       NM_018019               2209 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens mediator complex subunit 9 (MED9), mRNA.
ACCESSION   NM_018019 NM_138427
VERSION     NM_018019.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Franzese,M., Zanfardino,M., Soricelli,A., Coppola,A., Maiello,C.,
            Salvatore,M., Schiano,C. and Napoli,C.
  TITLE     Familial Dilated Cardiomyopathy: A Novel MED9 Short Isoform
            Identification
  JOURNAL   Int J Mol Sci 25 (5), 3057 (2024)
   PUBMED   38474301
  REMARK    GeneRIF: Familial Dilated Cardiomyopathy: A Novel MED9 Short
            Isoform Identification.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 2209)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 2209)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   5  (bases 1 to 2209)
  AUTHORS   Lambert,J.P., Tucholska,M., Go,C., Knight,J.D. and Gingras,A.C.
  TITLE     Proximity biotinylation and affinity purification are complementary
            approaches for the interactome mapping of chromatin-associated
            protein complexes
  JOURNAL   J Proteomics 118, 81-94 (2015)
   PUBMED   25281560
REFERENCE   6  (bases 1 to 2209)
  AUTHORS   Tsai,K.L., Tomomori-Sato,C., Sato,S., Conaway,R.C., Conaway,J.W.
            and Asturias,F.J.
  TITLE     Subunit architecture and functional modular rearrangements of the
            transcriptional mediator complex
  JOURNAL   Cell 157 (6), 1430-1444 (2014)
   PUBMED   24882805
  REMARK    Erratum:[Cell. 2014 Jul 17;158(2):463]
REFERENCE   7  (bases 1 to 2209)
  AUTHORS   Sato,S., Tomomori-Sato,C., Parmely,T.J., Florens,L., Zybailov,B.,
            Swanson,S.K., Banks,C.A., Jin,J., Cai,Y., Washburn,M.P.,
            Conaway,J.W. and Conaway,R.C.
  TITLE     A set of consensus mammalian mediator subunits identified by
            multidimensional protein identification technology
  JOURNAL   Mol Cell 14 (5), 685-691 (2004)
   PUBMED   15175163
REFERENCE   8  (bases 1 to 2209)
  AUTHORS   Tomomori-Sato,C., Sato,S., Parmely,T.J., Banks,C.A., Sorokina,I.,
            Florens,L., Zybailov,B., Washburn,M.P., Brower,C.S., Conaway,R.C.
            and Conaway,J.W.
  TITLE     A mammalian mediator subunit that shares properties with
            Saccharomyces cerevisiae mediator subunit Cse2
  JOURNAL   J Biol Chem 279 (7), 5846-5851 (2004)
   PUBMED   14638676
  REMARK    GeneRIF: the FLJ10193 protein, which we designate Med25, is a bona
            fide subunit of the mammalian Mediator complex
REFERENCE   9  (bases 1 to 2209)
  AUTHORS   Bi,W., Yan,J., Stankiewicz,P., Park,S.S., Walz,K., Boerkoel,C.F.,
            Potocki,L., Shaffer,L.G., Devriendt,K., Nowaczyk,M.J., Inoue,K. and
            Lupski,J.R.
  TITLE     Genes in a refined Smith-Magenis syndrome critical deletion
            interval on chromosome 17p11.2 and the syntenic region of the mouse
  JOURNAL   Genome Res 12 (5), 713-728 (2002)
   PUBMED   11997338
REFERENCE   10 (bases 1 to 2209)
  AUTHORS   Mittler,G., Kremmer,E., Timmers,H.T. and Meisterernst,M.
  TITLE     Novel critical role of a human Mediator complex for basal RNA
            polymerase II transcription
  JOURNAL   EMBO Rep 2 (9), 808-813 (2001)
   PUBMED   11559591
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC073621.21.
            
            On Nov 25, 2018 this sequence version replaced NM_018019.2.
            
            Summary: The multiprotein Mediator complex is a coactivator
            required for activation of RNA polymerase II transcription by DNA
            bound transcription factors. The protein encoded by this gene is
            thought to be a subunit of the Mediator complex. This gene is
            located within the Smith-Magenis syndrome region on chromosome 17.
            [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC000647.2, SRR1803612.23972.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000268711.4/ ENSP00000268711.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-266               AC073621.21        22083-22348         c
            267-2209            AC073621.21        6127-8069           c
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p11.2"
     gene            1..2209
                     /gene="MED9"
                     /gene_synonym="MED25"
                     /note="mediator complex subunit 9"
                     /db_xref="GeneID:55090"
                     /db_xref="HGNC:HGNC:25487"
                     /db_xref="MIM:609878"
     exon            1..266
                     /gene="MED9"
                     /gene_synonym="MED25"
                     /inference="alignment:Splign:2.1.0"
     CDS             43..483
                     /gene="MED9"
                     /gene_synonym="MED25"
                     /note="mediator subunit 25; mediator of RNA polymerase II
                     transcription, subunit 9 homolog"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 9"
                     /protein_id="NP_060489.1"
                     /db_xref="CCDS:CCDS11184.1"
                     /db_xref="GeneID:55090"
                     /db_xref="HGNC:HGNC:25487"
                     /db_xref="MIM:609878"
                     /translation="MASAGVAAGRQAEDVLPPTSDQPLPDTKPLPPPQPPPVPAPQPQ
                     QSPAPRPQSPARAREEENYSFLPLVHNIIKCMDKDSPEVHQDLNALKSKFQEMRKLIS
                     TMPGIHLSPEQQQQQLQSLREQVRTKNELLQKYKSLCMFEIPKE"
     exon            267..2209
                     /gene="MED9"
                     /gene_synonym="MED25"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2178..2183
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED9"
                     /gene_synonym="MED25"
                     /note="hexamer: ATTAAA"
     polyA_site      2209
                     /gene="MED9"
                     /gene_synonym="MED25"
                     /note="major polyA site"
ORIGIN      
        1 gcttttggcg acccgacctc tggctaacct acccccggag ccatggcctc tgctggggtg
       61 gcagccgggc gacaggcgga ggatgtattg ccgccaacgt ccgaccagcc gctgcctgac
      121 accaagccgc tgccgcctcc tcagccgccg ccggtccctg cgcctcaacc gcagcagtcg
      181 ccggcgccac ggcctcagtc acctgcccgc gcgagggagg aagagaacta ctccttttta
      241 cctttggttc acaacatcat caaatgcatg gacaaggaca gcccggaggt ccaccaggac
      301 ctgaacgccc tcaaaagcaa gttccaggag atgcgcaagc tcatcagcac catgcccggc
      361 atccacctga gccccgaaca gcagcagcag cagctgcaga gcctccggga gcaagtcagg
      421 accaagaatg agcttctgca aaagtacaag agcctctgca tgttcgaaat ccccaaggag
      481 tagagtgagg ctgacttcct tagaaagagg gggaagccaa tggcctgtct ccccactacc
      541 atccccaaac gctccttggg gcgtggttcc tgtggacccc agctcagctc gtcaagctgc
      601 aggggcgggg ctcctgtgct gctgcgcgcg cttcgcctgt gcgggagcca gcgcagagct
      661 tggctgcgcc gggggttcct cgtgtagatc catatgtcta gatgcataat aactggagtg
      721 cctgctggtg gaagtcagaa tgctcctgga ggctgcagag ggggtggagg actctcccct
      781 gcctctgggg agggggccat ctgctgcgcc cggccccact gacagatctg aagagcacag
      841 taggaaggga ggcggctcct ctttgcttcc ttccctctct ctcctcccac ccccatagga
      901 tcagtgtgta ccaggtacac attgttcctg ttaacagcag cttcttgaaa catttgcata
      961 gaattcactg gacgaattaa gcctgcactc atatggcata gaattgtgag agaatgtttt
     1021 gaaaggccag agggtggcct ttttccccaa acagtttggt tccttttatg tttgagccag
     1081 tgaagggaac tacgctttgg gggcttcagc ctagagccct gccaggcagc ccctggctcc
     1141 aggttccctg cctcctagcg ctctcctcgc cttcagctct tgctcccttc ctcgttcatc
     1201 accctcagtc agtgcccaag agtggccaaa ccgcttcaca tctgcagtgc ttccccaggg
     1261 ttgacaaggg gccgtccttt ccacacaggc cagaagaggt cttcaggcga accgaccttc
     1321 ccccttctgg catttcagat tccccttgct ctggttaaaa ggtctttccc tcgtggcctt
     1381 tgcacttgcg gcagcaacgt gtactacact gcagaagggt tcagtatgca ccttgtgttg
     1441 agagagaggc aaccctgggg gccagttcag gtggtcccca accataagct aggtctgaaa
     1501 gttacacagc caagtttgag ctcttaaaag ttgatgaaca gcctcatttc cccagcttcc
     1561 ctgatttctt ccagatggga cgttttattt gtgtgctctc ccttgactgt cagattgaag
     1621 taagagcagt tctctccgtt gcctctcgag gaggaggtgc gaagtcctgg agtattgttt
     1681 gggtctcgga atgggcgcat aacctgcgct gaccagttta ggggcttagc agatgcctgc
     1741 cagctgacct cgttggcagg agggttgggt ggagatgttt ttagcagagc ttccattagt
     1801 gtagacctgt agccacctgt cagaaggtgg gtggcatatt ggggacctgg gaatgtgtga
     1861 aggaggagat caaatttcag tggctttgga cagaaaagaa ggctctggat ttaagcgggt
     1921 ggtcacctgt gagaccaggt ctaccttggg actgttattt aactgaatca gttatttcct
     1981 tgaaatttca cagtagtggg tgggcctgtt ttaaggctct gacagatacc acgaaacatg
     2041 aagcacgtgg aactacaaga cccccggggt ctttctgagt gcaaggctga aatggacaag
     2101 ggctcctcac gggggtggag ggagccggag cctgccttgt gttccttttt tgacttgtga
     2161 catttttcaa acacataatt aaaaggactt atgctctgct gtctcagga
//
