LOCUS       NM_201542               1968 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens mediator complex subunit 8 (MED8), transcript variant
            5, mRNA.
ACCESSION   NM_201542 NM_001001654
VERSION     NM_201542.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 1968)
  AUTHORS   Fasci,D., van Ingen,H., Scheltema,R.A. and Heck,A.J.R.
  TITLE     Histone Interaction Landscapes Visualized by Crosslinking Mass
            Spectrometry in Intact Cell Nuclei
  JOURNAL   Mol Cell Proteomics 17 (10), 2018-2033 (2018)
   PUBMED   30021884
REFERENCE   4  (bases 1 to 1968)
  AUTHORS   Tsai,K.L., Tomomori-Sato,C., Sato,S., Conaway,R.C., Conaway,J.W.
            and Asturias,F.J.
  TITLE     Subunit architecture and functional modular rearrangements of the
            transcriptional mediator complex
  JOURNAL   Cell 157 (6), 1430-1444 (2014)
   PUBMED   24882805
  REMARK    Erratum:[Cell. 2014 Jul 17;158(2):463]
REFERENCE   5  (bases 1 to 1968)
  AUTHORS   Wu,F., Xing,J., Wang,S., Li,M. and Zheng,C.
  TITLE     Screening and identification of host factors interacting with UL14
            of herpes simplex virus 1
  JOURNAL   Med Microbiol Immunol 200 (3), 203-208 (2011)
   PUBMED   21512757
REFERENCE   6  (bases 1 to 1968)
  AUTHORS   Brower,C.S., Sato,S., Tomomori-Sato,C., Kamura,T., Pause,A.,
            Stearman,R., Klausner,R.D., Malik,S., Lane,W.S., Sorokina,I.,
            Roeder,R.G., Conaway,J.W. and Conaway,R.C.
  TITLE     Mammalian mediator subunit mMED8 is an Elongin BC-interacting
            protein that can assemble with Cul2 and Rbx1 to reconstitute a
            ubiquitin ligase
  JOURNAL   Proc Natl Acad Sci U S A 99 (16), 10353-10358 (2002)
   PUBMED   12149480
  REMARK    GeneRIF: mammalian mediator subunit mMED8 is an elongin
            BC-interacting protein that can assemble with Cul2 and Rbx1
            reconstitute a ubiquitin ligase (Ubc5; E2 ubiquitin-conjugating
            enzyme Ubc5)
REFERENCE   7  (bases 1 to 1968)
  AUTHORS   Spahr,H., Samuelsen,C.O., Baraznenok,V., Ernest,I., Huylebroeck,D.,
            Remacle,J.E., Samuelsson,T., Kieselbach,T., Holmberg,S. and
            Gustafsson,C.M.
  TITLE     Analysis of Schizosaccharomyces pombe mediator reveals a set of
            essential subunits conserved between yeast and metazoan cells
  JOURNAL   Proc Natl Acad Sci U S A 98 (21), 11985-11990 (2001)
   PUBMED   11572939
REFERENCE   8  (bases 1 to 1968)
  AUTHORS   Mittler,G., Kremmer,E., Timmers,H.T. and Meisterernst,M.
  TITLE     Novel critical role of a human Mediator complex for basal RNA
            polymerase II transcription
  JOURNAL   EMBO Rep 2 (9), 808-813 (2001)
   PUBMED   11559591
REFERENCE   9  (bases 1 to 1968)
  AUTHORS   Naar,A.M., Beaurang,P.A., Zhou,S., Abraham,S., Solomon,W. and
            Tjian,R.
  TITLE     Composite co-activator ARC mediates chromatin-directed
            transcriptional activation
  JOURNAL   Nature 398 (6730), 828-832 (1999)
   PUBMED   10235267
REFERENCE   10 (bases 1 to 1968)
  AUTHORS   Jiang,Y.W., Veschambre,P., Erdjument-Bromage,H., Tempst,P.,
            Conaway,J.W., Conaway,R.C. and Kornberg,R.D.
  TITLE     Mammalian mediator of transcriptional regulation and its possible
            role as an end-point of signal transduction pathways
  JOURNAL   Proc Natl Acad Sci U S A 95 (15), 8538-8543 (1998)
   PUBMED   9671713
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG620658.1, AF521562.1,
            BC010019.2, CB162354.1, AL139289.6 and BF196855.1.
            
            On Nov 23, 2018 this sequence version replaced NM_201542.4.
            
            Summary: This gene encodes a protein component of the mediator
            complex, which aids in transcriptional activation through
            interaction with RNA polymerase II and gene-specific transcription
            factors. The encoded protein may also function in ubiquitin
            ligation and protein degradation. Alternatively spliced transcript
            variants encoding multiple isoforms have been observed for this
            gene. [provided by RefSeq, Jan 2013].
            
            Transcript Variant: This variant (5) uses an alternate exon
            structure in the 3' coding region and differs in the 3' UTR,
            compared to variant 3. The resulting isoform (4) has a shorter
            C-terminus, compared to isoform 3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189652.234820.1,
                                           SRR3476690.715714.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN04284274 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000372457.9/ ENSP00000361535.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-42                BG620658.1         14-55
            43-590              AF521562.1         7-554
            591-1159            BC010019.2         618-1186
            1160-1638           CB162354.1         72-550
            1639-1679           AL139289.6         65136-65176         c
            1680-1968           BF196855.1         13-301              c
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p34.2"
     gene            1..1968
                     /gene="MED8"
                     /gene_synonym="ARC32"
                     /note="mediator complex subunit 8"
                     /db_xref="GeneID:112950"
                     /db_xref="HGNC:HGNC:19971"
                     /db_xref="MIM:607956"
     exon            1..42
                     /gene="MED8"
                     /gene_synonym="ARC32"
                     /inference="alignment:Splign:2.1.0"
     CDS             37..843
                     /gene="MED8"
                     /gene_synonym="ARC32"
                     /note="isoform 4 is encoded by transcript variant 5;
                     mediator of RNA polymerase II transcription subunit MED8;
                     activator-recruited cofactor 32 kDa component; mediator of
                     RNA polymerase II transcription subunit 8"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 8 isoform 4"
                     /protein_id="NP_963836.2"
                     /db_xref="CCDS:CCDS487.2"
                     /db_xref="GeneID:112950"
                     /db_xref="HGNC:HGNC:19971"
                     /db_xref="MIM:607956"
                     /translation="MQREEKQLEASLDALLSQVADLKNSLGSFICKLENEYGRLTWPS
                     VLDSFALLSGQLNTLNKVLKHEKTPLFRNQVIIPLVLSPDRDEDLMRQTEGRVPVFSH
                     EVVPDHLRTKPDPEVEEQEKQLTTDAARIGADAAQKQIQSLNKMCSNLLEKISKEERE
                     SESGGLRPNKQTFNPTDTNALVAAVAFGKGLSNWRPSGSSGPGQAGQPGAGTILAGTS
                     GLQQVQMAGAPSQQQPMLSGVQMAQAGQPGKMPSGIKTNIKSASMHPYQR"
     exon            43..161
                     /gene="MED8"
                     /gene_synonym="ARC32"
                     /inference="alignment:Splign:2.1.0"
     exon            162..306
                     /gene="MED8"
                     /gene_synonym="ARC32"
                     /inference="alignment:Splign:2.1.0"
     exon            307..447
                     /gene="MED8"
                     /gene_synonym="ARC32"
                     /inference="alignment:Splign:2.1.0"
     exon            448..529
                     /gene="MED8"
                     /gene_synonym="ARC32"
                     /inference="alignment:Splign:2.1.0"
     exon            530..778
                     /gene="MED8"
                     /gene_synonym="ARC32"
                     /inference="alignment:Splign:2.1.0"
     exon            779..1968
                     /gene="MED8"
                     /gene_synonym="ARC32"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1140..1145
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED8"
                     /gene_synonym="ARC32"
                     /note="hexamer: AATAAA"
     polyA_site      1161
                     /gene="MED8"
                     /gene_synonym="ARC32"
                     /note="major polyA site"
     regulatory      1949..1954
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED8"
                     /gene_synonym="ARC32"
                     /note="hexamer: AATAAA"
     polyA_site      1968
                     /gene="MED8"
                     /gene_synonym="ARC32"
                     /note="major polyA site"
ORIGIN      
        1 ggaagtgaaa tcgtggcagc cgcctcggcc gccgcaatgc agagagagga gaagcagctt
       61 gaggcatcat tagatgcact gctgagtcaa gtggctgatc tgaagaactc tctggggagt
      121 ttcatttgca agttggagaa cgagtatggc cggctgacct ggccatctgt cctggacagc
      181 tttgccttgc tttctggaca gctgaacact ctgaacaagg tcttgaagca tgaaaaaaca
      241 ccgctgttcc gtaaccaggt catcattcct ctggtgttgt ctccagaccg agatgaagat
      301 ctcatgcggc agactgaagg acgggtgcct gttttcagcc atgaggtagt ccctgaccat
      361 ctgagaacca agcctgaccc tgaagtggaa gaacaggaga agcaactgac gacagatgct
      421 gcccgcattg gtgcagatgc agcccagaag cagatccaga gcttgaataa aatgtgttca
      481 aaccttctgg agaaaatcag caaagaggag cgagaatcag agagtggagg tctccggccg
      541 aacaagcaga cctttaaccc tacagacact aatgccttgg tggcagctgt tgcctttggg
      601 aaaggactat ctaattggag accttcaggc agcagtggtc ctggccaggc aggccagcca
      661 ggagctggga cgatccttgc aggaacctca ggattacagc aggtgcagat ggcaggagct
      721 ccaagccagc agcagccaat gctcagtggg gtacaaatgg ctcaggcagg tcaaccaggg
      781 aaaatgccaa gtggaataaa aaccaacatc aagtcggctt ccatgcatcc ctaccagcgg
      841 tgagtgtggc tggcaacctc gactccctgg tgctctttgc agagttgggc agtgaaatta
      901 ccttttgctc aaggctcacc tagatgggta caataaaaag aacatgggct ttcagcagca
      961 gacaaatccc acttccacca ctgactagct gtgtgacctt ggacaagtga cctaattttt
     1021 ctgagcctgt ttctcatttg taaatggtga taatacctac ctcatagggt tgttgtgagg
     1081 attaaaatga ggaaatgaat gtaaagcact tagtacagta tatgaaataa tgggtattca
     1141 ataaatgata gtttctacag atccttctcc ccaccaccct ccacagtcct tgatccagaa
     1201 cttaccctaa tctgatactg cctcacgtca atggtgagct gatggacaca agatcaaata
     1261 aggctatgct tattttgtgc tgccagaaac tgtagcaacc tctgtgttct tagaggcaca
     1321 ctgtttttgc aggccctcct gcctgggttt cattctggct atccctctaa ggcgcaaggt
     1381 gaagaagctt ctgggccagg aaggaaaaaa aaatgcccac ctgcagctct ggtgaagctt
     1441 gggcctgctc tcctttccat cctctaagga gccaacttgg cttttacctg tcaaatagtc
     1501 ataaagtccc ctatccttta ccccacctta tacacacgag gctttctcag gaagtggctc
     1561 tgccaggcag gactatgtgg gaaagggttt ttccttagca cacgaaaaag ccccttcccc
     1621 tggattcatg tttcttattt tggagggaga agggaattgc acttcacact gccatcaggg
     1681 tttagttgac ctcataatgg tgcccacttt ctcgactttg gccaggattt ccttcaaaga
     1741 aaacgacttt ccttcatttc cctaagcctg tggcccaaat ggtggaccag aatgatggtg
     1801 ggagggggca acccccagta gctttgcctg cttttataaa gttgaacaaa ttgaatttag
     1861 acattcaggc taacctgcct ttcttagtac tcctttgttg gcatgggcag gggttgagtc
     1921 agcagaagtg gaccaaagga ttcctctgaa taaagttatt taaattga
//
