LOCUS       NM_004270               2071 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens mediator complex subunit 7 (MED7), transcript variant
            2, mRNA.
ACCESSION   NM_004270
VERSION     NM_004270.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Chen,Z.L., Ma,Y.Y., Mou,X.Z. and Zhang,J.G.
  TITLE     Upregulation of MED7 was associated with progression in
            hepatocellular carcinoma
  JOURNAL   Cancer Biomark 38 (4), 603-611 (2023)
   PUBMED   38073375
  REMARK    GeneRIF: Upregulation of MED7 was associated with progression in
            hepatocellular carcinoma.
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 2071)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 2071)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 2071)
  AUTHORS   Joseph,C., Macnamara,O., Craze,M., Russell,R., Provenzano,E.,
            Nolan,C.C., Diez-Rodriguez,M., Sonbul,S.N., Aleskandarany,M.A.,
            Green,A.R., Rakha,E.A., Ellis,I.O. and Mukherjee,A.
  TITLE     Mediator complex (MED) 7: a biomarker associated with good
            prognosis in invasive breast cancer, especially ER+ luminal
            subtypes
  JOURNAL   Br J Cancer 118 (8), 1142-1151 (2018)
   PUBMED   29588513
  REMARK    GeneRIF: MED7 is an important prognostic marker in Breast cancer,
            particularly in ER+luminal subtypes, associated with improved
            survival and warrants future functional analysis.
REFERENCE   6  (bases 1 to 2071)
  AUTHORS   Ryu,S. and Tjian,R.
  TITLE     Purification of transcription cofactor complex CRSP
  JOURNAL   Proc Natl Acad Sci U S A 96 (13), 7137-7142 (1999)
   PUBMED   10377381
REFERENCE   7  (bases 1 to 2071)
  AUTHORS   Naar,A.M., Beaurang,P.A., Zhou,S., Abraham,S., Solomon,W. and
            Tjian,R.
  TITLE     Composite co-activator ARC mediates chromatin-directed
            transcriptional activation
  JOURNAL   Nature 398 (6730), 828-832 (1999)
   PUBMED   10235267
REFERENCE   8  (bases 1 to 2071)
  AUTHORS   Ryu,S., Zhou,S., Ladurner,A.G. and Tjian,R.
  TITLE     The transcriptional cofactor complex CRSP is required for activity
            of the enhancer-binding protein Sp1
  JOURNAL   Nature 397 (6718), 446-450 (1999)
   PUBMED   9989412
REFERENCE   9  (bases 1 to 2071)
  AUTHORS   Jiang,Y.W., Veschambre,P., Erdjument-Bromage,H., Tempst,P.,
            Conaway,J.W., Conaway,R.C. and Kornberg,R.D.
  TITLE     Mammalian mediator of transcriptional regulation and its possible
            role as an end-point of signal transduction pathways
  JOURNAL   Proc Natl Acad Sci U S A 95 (15), 8538-8543 (1998)
   PUBMED   9671713
REFERENCE   10 (bases 1 to 2071)
  AUTHORS   Myers,L.C., Gustafsson,C.M., Bushnell,D.A., Lui,M.,
            Erdjument-Bromage,H., Tempst,P. and Kornberg,R.D.
  TITLE     The Med proteins of yeast and their function through the RNA
            polymerase II carboxy-terminal domain
  JOURNAL   Genes Dev 12 (1), 45-54 (1998)
   PUBMED   9420330
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AV711090.1, AF031383.1,
            BQ011916.1 and AC010609.7.
            
            On Nov 24, 2018 this sequence version replaced NM_004270.4.
            
            Summary: The activation of gene transcription is a multistep
            process that is triggered by factors that recognize transcriptional
            enhancer sites in DNA. These factors work with co-activators to
            direct transcriptional initiation by the RNA polymerase II
            apparatus. The protein encoded by this gene is a subunit of the
            CRSP (cofactor required for SP1 activation) complex, which, along
            with TFIID, is required for efficient activation by SP1. This
            protein is also a component of other multisubunit complexes e.g.
            thyroid hormone receptor-(TR-) associated proteins which interact
            with TR and facilitate TR function on DNA templates in conjunction
            with initiation factors and cofactors. Two transcript variants
            encoding the same protein have been found for this gene. [provided
            by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Both variants encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.163372.1,
                                           SRR1803616.228038.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000286317.6/ ENSP00000286317.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-565               AV711090.1         58-622
            566-775             AF031383.1         569-778
            776-1055            BQ011916.1         17-296              c
            1056-2071           AC010609.7         19285-20300         c
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q33.3"
     gene            1..2071
                     /gene="MED7"
                     /gene_synonym="ARC34; CRSP33; CRSP9"
                     /note="mediator complex subunit 7"
                     /db_xref="GeneID:9443"
                     /db_xref="HGNC:HGNC:2378"
                     /db_xref="MIM:605045"
     exon            1..46
                     /gene="MED7"
                     /gene_synonym="ARC34; CRSP33; CRSP9"
                     /inference="alignment:Splign:2.1.0"
     exon            47..2071
                     /gene="MED7"
                     /gene_synonym="ARC34; CRSP33; CRSP9"
                     /inference="alignment:Splign:2.1.0"
     CDS             64..765
                     /gene="MED7"
                     /gene_synonym="ARC34; CRSP33; CRSP9"
                     /note="cofactor required for Sp1 transcriptional
                     activation, subunit 9 (33kD); cofactor required for Sp1
                     transcriptional activation, subunit 9, 33kDa;
                     transcriptional coactivator CRSP33; activator-recruited
                     cofactor 34 kDa component; cofactor required for Sp1
                     transcriptional activation subunit 9; RNA polymerase
                     transcriptional regulation mediator subunit 7 homolog;
                     CRSP complex subunit 9"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 7"
                     /protein_id="NP_004261.1"
                     /db_xref="CCDS:CCDS4334.1"
                     /db_xref="GeneID:9443"
                     /db_xref="HGNC:HGNC:2378"
                     /db_xref="MIM:605045"
                     /translation="MGEPQQVSALPPPPMQYIKEYTDENIQEGLAPKPPPPIKDSYMM
                     FGNQFQCDDLIIRPLESQGIERLHPMQFDHKKELRKLNMSILINFLDLLDILIRSPGS
                     IKREEKLEDLKLLFVHVHHLINEYRPHQARETLRVMMEVQKRQRLETAERFQKHLERV
                     IEMIQNCLASLPDDLPHSEAGMRVKTEPMDADDSNNCTGQNEHQRENSGHRRDQIIEK
                     DAALCVLIDEMNERP"
     regulatory      1031..1036
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED7"
                     /gene_synonym="ARC34; CRSP33; CRSP9"
                     /note="hexamer: AGTAAA"
     polyA_site      1055
                     /gene="MED7"
                     /gene_synonym="ARC34; CRSP33; CRSP9"
                     /note="major polyA site"
     regulatory      1170..1175
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED7"
                     /gene_synonym="ARC34; CRSP33; CRSP9"
                     /note="hexamer: ATTAAA"
     polyA_site      1197
                     /gene="MED7"
                     /gene_synonym="ARC34; CRSP33; CRSP9"
     regulatory      2028..2033
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED7"
                     /gene_synonym="ARC34; CRSP33; CRSP9"
                     /note="hexamer: AATAAA"
     polyA_site      2071
                     /gene="MED7"
                     /gene_synonym="ARC34; CRSP33; CRSP9"
ORIGIN      
        1 cggctgcaga ggggaaggcg gctaccagtg taaagccaga gctgaggttc ttgatagtcc
       61 acaatgggtg aaccacagca agtgagtgca cttccaccac ctccaatgca atatatcaag
      121 gaatatacgg atgaaaatat tcaagaaggc ttagctccca agcctccccc tccaataaaa
      181 gacagttaca tgatgtttgg caatcagttc caatgtgatg atcttatcat ccgccctttg
      241 gaaagtcagg gcatcgaacg gcttcatcct atgcagtttg atcacaagaa agaactgaga
      301 aaacttaata tgtctatcct tattaatttc ttggaccttt tagatatttt aataaggagc
      361 cctgggagta taaaacgaga agagaaacta gaagatctta agctgctttt tgtacacgtg
      421 catcatctta taaatgaata ccgaccccac caagcaagag agaccttgag agtcatgatg
      481 gaggtccaga aacgtcaacg gcttgaaaca gctgagagat ttcaaaagca cctggaacga
      541 gtaattgaaa tgattcagaa ttgcttggct tctttgcctg atgatttgcc tcattcagaa
      601 gcaggaatga gagtaaaaac tgaaccaatg gatgctgatg atagcaacaa ttgtactgga
      661 cagaatgaac atcaaagaga aaattcaggt cataggagag atcagattat agagaaagat
      721 gctgccttgt gtgtcctaat tgatgagatg aatgaaagac catgaaagat gtttcttttt
      781 ctttttttcc ttttgataat agcatcatat attagttcat tttcttttgg acagtcttaa
      841 gagaagtttc actaaaaatg taaacagctt taatcttgac tccaaatttt tcaattatga
      901 gatgtcatag gcagtaattt cgctgtataa caagcataga caaatgagtg tccctgcact
      961 aagaagaatc actttaaaaa gcaaagtgtt agctgctgtt gtatgggaca ttcctatgtt
     1021 ttagagttgc agtaaaactt tgatgataac ctcaataata gcaaagtttt cgtctttgaa
     1081 aaggggattt agcatttgct ttaagaatga tagataaatg gatattaagc tctctacatg
     1141 taaaactatg aaatctttag acttattcca ttaaaaattt tgcttaagct ccaaaaagta
     1201 gcataacatg ttgatagaga ggagcccagt agagttataa aatagaaact tcattttttc
     1261 ctcatgactg cttctgtaaa cccactagct cagtcttttc tccctatcct gaatggactc
     1321 ttgcagggaa gtcccataaa ttgttgtttt tttttccagt cactcaggta ataagtcctt
     1381 tggtcacttt aagttacaga cattaatttt agtaattaag atgtctgtgt cattagctat
     1441 acattttctt taaagctaag ctcagacttg tttaagtcca caatgggaaa ggggtttgat
     1501 ggcagagact gctaggtgtc taccaaagat tcttgctctt tgaagatatg ttgtcaagat
     1561 gtggcaatcc agccagggac atttccctac tcccagcatc tctgtgagaa catatgacta
     1621 agtcgtaggc ggtgaaatgt gggcatagga atccaggctt gccccataaa catttcttgc
     1681 atgatcctcc attctcttct cccagctgct ggttggacgg tcgtatccag gataagtttg
     1741 gaagccaccg aagatggcaa agattgtcag tctggttctt tgaatgaccg tgaggactaa
     1801 gtcttccttt cagtaactcc actctgcccc tagctaattg gactttatat gagcactttc
     1861 ttctcttctt cccccgactg tccatctgct cttccaccaa ggtgttagtg gagagttgac
     1921 cttgtgtctg aggatctctg cctgccgttt tcttaatgta ggcccgtggt tcttaaagtg
     1981 tgtggtcctg gaccagcagc attggtatca cttggggact tactagaaat aaaagtttta
     2041 gtataatccg taggttcaga aaaataaaaa a
//
