LOCUS       NM_005466               2351 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens mediator complex subunit 6 (MED6), transcript variant
            2, mRNA.
ACCESSION   NM_005466
VERSION     NM_005466.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 2351)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   4  (bases 1 to 2351)
  AUTHORS   Marcon,E., Ni,Z., Pu,S., Turinsky,A.L., Trimble,S.S., Olsen,J.B.,
            Silverman-Gavrila,R., Silverman-Gavrila,L., Phanse,S., Guo,H.,
            Zhong,G., Guo,X., Young,P., Bailey,S., Roudeva,D., Zhao,D.,
            Hewel,J., Li,J., Graslund,S., Paduch,M., Kossiakoff,A.A.,
            Lupien,M., Emili,A., Wodak,S.J. and Greenblatt,J.
  TITLE     Human-chromatin-related protein interactions identify a demethylase
            complex required for chromosome segregation
  JOURNAL   Cell Rep 8 (1), 297-310 (2014)
   PUBMED   24981860
REFERENCE   5  (bases 1 to 2351)
  AUTHORS   Tsai,K.L., Tomomori-Sato,C., Sato,S., Conaway,R.C., Conaway,J.W.
            and Asturias,F.J.
  TITLE     Subunit architecture and functional modular rearrangements of the
            transcriptional mediator complex
  JOURNAL   Cell 157 (6), 1430-1444 (2014)
   PUBMED   24882805
  REMARK    Erratum:[Cell. 2014 Jul 17;158(2):463]
REFERENCE   6  (bases 1 to 2351)
  AUTHORS   Ito,M., Yuan,C.X., Malik,S., Gu,W., Fondell,J.D., Yamamura,S.,
            Fu,Z.Y., Zhang,X., Qin,J. and Roeder,R.G.
  TITLE     Identity between TRAP and SMCC complexes indicates novel pathways
            for the function of nuclear receptors and diverse mammalian
            activators
  JOURNAL   Mol Cell 3 (3), 361-370 (1999)
   PUBMED   10198638
REFERENCE   7  (bases 1 to 2351)
  AUTHORS   Gu,W., Malik,S., Ito,M., Yuan,C.X., Fondell,J.D., Zhang,X.,
            Martinez,E., Qin,J. and Roeder,R.G.
  TITLE     A novel human SRB/MED-containing cofactor complex, SMCC, involved
            in transcription regulation
  JOURNAL   Mol Cell 3 (1), 97-108 (1999)
   PUBMED   10024883
  REMARK    Erratum:[Mol Cell 1999 Apr;3(4):following 541]
REFERENCE   8  (bases 1 to 2351)
  AUTHORS   Sun,X., Zhang,Y., Cho,H., Rickert,P., Lees,E., Lane,W. and
            Reinberg,D.
  TITLE     NAT, a human complex containing Srb polypeptides that functions as
            a negative regulator of activated transcription
  JOURNAL   Mol Cell 2 (2), 213-222 (1998)
   PUBMED   9734358
REFERENCE   9  (bases 1 to 2351)
  AUTHORS   Jiang,Y.W., Veschambre,P., Erdjument-Bromage,H., Tempst,P.,
            Conaway,J.W., Conaway,R.C. and Kornberg,R.D.
  TITLE     Mammalian mediator of transcriptional regulation and its possible
            role as an end-point of signal transduction pathways
  JOURNAL   Proc Natl Acad Sci U S A 95 (15), 8538-8543 (1998)
   PUBMED   9671713
REFERENCE   10 (bases 1 to 2351)
  AUTHORS   Lee,Y.C., Min,S., Gim,B.S. and Kim,Y.J.
  TITLE     A transcriptional mediator protein that is required for activation
            of many RNA polymerase II promoters and is conserved from yeast to
            humans
  JOURNAL   Mol Cell Biol 17 (8), 4622-4632 (1997)
   PUBMED   9234719
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            HY064772.1, BC004106.2, AL357153.4, BM999935.1 and DA869309.1.
            
            On Feb 27, 2020 this sequence version replaced NM_005466.3.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site, compared to variant 1. The encoded isoform (2) is
            shorter than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK223346.1, AK290647.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1968540 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000256379.10/ ENSP00000256379.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-62                HY064772.1         37-98
            63-1332             BC004106.2         72-1341
            1333-1388           AL357153.4         125626-125681       c
            1389-1983           BM999935.1         19-613              c
            1984-2351           DA869309.1         172-539
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q24.2"
     gene            1..2351
                     /gene="MED6"
                     /gene_synonym="ARC33; NY-REN-28"
                     /note="mediator complex subunit 6"
                     /db_xref="GeneID:10001"
                     /db_xref="HGNC:HGNC:19970"
                     /db_xref="MIM:602984"
     exon            1..40
                     /gene="MED6"
                     /gene_synonym="ARC33; NY-REN-28"
                     /inference="alignment:Splign:2.1.0"
     CDS             19..759
                     /gene="MED6"
                     /gene_synonym="ARC33; NY-REN-28"
                     /note="isoform 2 is encoded by transcript variant 2;
                     mediator of RNA polymerase II transcription subunit 6;
                     renal carcinoma antigen NY-REN-28; activator-recruited
                     cofactor 33 kDa component; CTD-2540L5.5"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 6 isoform 2"
                     /protein_id="NP_005457.2"
                     /db_xref="CCDS:CCDS9805.1"
                     /db_xref="GeneID:10001"
                     /db_xref="HGNC:HGNC:19970"
                     /db_xref="MIM:602984"
                     /translation="MAAVDIRDNLLGISWVDSSWIPILNSGSVLDYFSERSNPFYDRT
                     CNNEVVKMQRLTLEHLNQMVGIEYILLHAQEPILFIIRKQQRQSPAQVIPLADYYIIA
                     GVIYQAPDLGSVINSRVLTAVHGIQSAFDEAMSYCRYHPSKGYWWHFKDHEEQDKVRP
                     KAKRKEEPSSIFQRQRVDALLLDLRQKFPPKFVQLKPGEKPVPVDQTKKEAEPIPETV
                     KPEEKETTKNVQQTVSAKGPPEKRMRLQ"
     exon            41..200
                     /gene="MED6"
                     /gene_synonym="ARC33; NY-REN-28"
                     /inference="alignment:Splign:2.1.0"
     exon            201..292
                     /gene="MED6"
                     /gene_synonym="ARC33; NY-REN-28"
                     /inference="alignment:Splign:2.1.0"
     exon            293..375
                     /gene="MED6"
                     /gene_synonym="ARC33; NY-REN-28"
                     /inference="alignment:Splign:2.1.0"
     exon            376..484
                     /gene="MED6"
                     /gene_synonym="ARC33; NY-REN-28"
                     /inference="alignment:Splign:2.1.0"
     exon            485..600
                     /gene="MED6"
                     /gene_synonym="ARC33; NY-REN-28"
                     /inference="alignment:Splign:2.1.0"
     exon            601..628
                     /gene="MED6"
                     /gene_synonym="ARC33; NY-REN-28"
                     /inference="alignment:Splign:2.1.0"
     exon            629..2351
                     /gene="MED6"
                     /gene_synonym="ARC33; NY-REN-28"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1315..1320
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED6"
                     /gene_synonym="ARC33; NY-REN-28"
                     /note="hexamer: AATAAA"
     polyA_site      1342
                     /gene="MED6"
                     /gene_synonym="ARC33; NY-REN-28"
                     /note="major polyA site"
     regulatory      1963..1968
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED6"
                     /gene_synonym="ARC33; NY-REN-28"
                     /note="hexamer: ATTATA"
     polyA_site      1983
                     /gene="MED6"
                     /gene_synonym="ARC33; NY-REN-28"
ORIGIN      
        1 gtaaacgctc tcggaattat ggcggcggtg gatatccgag acaatctgct gggaatttct
       61 tgggttgaca gctcttggat ccctattttg aacagtggta gtgtcctgga ttacttttca
      121 gaaagaagta atccttttta tgacagaaca tgtaataatg aagtggtcaa aatgcagagg
      181 ctaacattag aacacttgaa tcagatggtt ggaatcgagt acatcctttt gcatgctcaa
      241 gagcccattc ttttcatcat tcggaagcaa cagcggcagt cccctgccca agttatccca
      301 ctagctgatt actatatcat tgctggagtg atctatcagg caccagactt gggatcagtt
      361 ataaactcta gagtgcttac tgcagtgcat ggtattcagt cagcttttga tgaagctatg
      421 tcatactgtc gatatcatcc ttccaaaggg tattggtggc acttcaaaga tcatgaagag
      481 caagataaag tcagacctaa agccaaaagg aaagaagaac caagctctat ttttcagaga
      541 caacgtgtgg atgctttact tttagacctc agacaaaaat ttccacccaa atttgtgcag
      601 ctaaagcctg gagaaaagcc tgttccagtg gatcaaacaa agaaagaggc agaacctata
      661 ccagaaactg taaaacctga ggagaaggag accacaaaga atgtacaaca gacagtgagt
      721 gctaaaggcc cccctgaaaa acggatgaga cttcagtgag tactggacaa aagagaagcc
      781 tggaagactc ctcatgctag ttatcatacc tcagtactgt ggctcttgag ctttgaagta
      841 ctttattgta accttcttat ttgtatggaa tgcgcttatt ttttgaaagg atattaggcc
      901 ggatgtggtg gctcacgcct gtaatcccag cactttggga ggccatggcg ggtggatcac
      961 ttgaggtcag aagttcaaga ccagcctgac caatatggtg aaaccccgtc tctactaaaa
     1021 atacaaaaat tagccgggcg tggtggcggg cgcccatagt cccagctact cgggaggctg
     1081 agacaggaga cttgcttgaa cccgggaggt ggaggttgcc ctgagctgat tatcatgctg
     1141 ttgcactcca gcttgggcga cagagcgaga ctttgtctca aaaaagaaga aaagatatta
     1201 ttcccatcat gatttcttgt gaatatttgt tatatgtctt ctgtaacctt tcctctcccg
     1261 gacttgagca acctacacac tcacatgttt actggtagat atgtttaaaa gcaaaataaa
     1321 ggtatttgta tatattgctt catcatgact tttttcttca tcataactga tatttttcag
     1381 atgcactttg aagacataaa ggatgttaaa gtagtaacta agcatatttc tttttgttca
     1441 gttacataat cctcagtatt aaaataaaca gttgctgaca ttttggaaga tgaagaaaag
     1501 gaaaaacctc actcaccaat accattaccc tataaatact gtaacagtgt tgtgaatttt
     1561 aatcttattc tgtgtgtaga tacttctttt ttcaataaag aaaaggaatt ttattttttt
     1621 taaagttatg gaggctgaga agtccagcat tgattggctc catctggtga gggccttctt
     1681 gctggtagga agtctgcaaa gtccctaggc agcacagggt atctcatggc aagggggctg
     1741 agtgtgcttg ctgcatgtag atgcttttaa aactgtactt taatcattcc tttcgtacaa
     1801 ttttacttta ttatatctgt tgtaagcttt tccccatgtt tatgtagttt ttatagactg
     1861 ttttagtgtc cgaatttctg taaatgattg tatctcagtt ttctttctgt cttatatttg
     1921 ggatttttcc cagtttacat tattttatat aagactgctg tgattatatc tttgtataat
     1981 gccttttcag tgtttggtat ttatttcctt aggatagagt tccagaagtg gaatcactag
     2041 agtctctgca tttttattgc tcttagtcca tattgctaag ttgttcctca aaatgttgga
     2101 ctagtgatca ctcaaacagc gaagtttgaa agtatgtatc aggttttcca gaattgctac
     2161 cactgaattt tgtcatttat gtttttagtg ttaagtagca aaagtggtat tttagtattt
     2221 aaagtagttg aagaatttct catctttttc tgcttttatg aaatgtctaa ttttatgtat
     2281 tgtgtgagat ttttgtcttt caacttctta ttttgacata atttccggca aacagaaaag
     2341 ttgtaagaat a
//
