LOCUS       NM_014166               2254 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens mediator complex subunit 4 (MED4), transcript variant
            1, mRNA.
ACCESSION   NM_014166
VERSION     NM_014166.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 2254)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 2254)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   5  (bases 1 to 2254)
  AUTHORS   Zhong,Q., Pevzner,S.J., Hao,T., Wang,Y., Mosca,R., Menche,J.,
            Taipale,M., Tasan,M., Fan,C., Yang,X., Haley,P., Murray,R.R.,
            Mer,F., Gebreab,F., Tam,S., MacWilliams,A., Dricot,A., Reichert,P.,
            Santhanam,B., Ghamsari,L., Calderwood,M.A., Rolland,T.,
            Charloteaux,B., Lindquist,S., Barabasi,A.L., Hill,D.E., Aloy,P.,
            Cusick,M.E., Xia,Y., Roth,F.P. and Vidal,M.
  TITLE     An inter-species protein-protein interaction network across vast
            evolutionary distance
  JOURNAL   Mol Syst Biol 12 (4), 865 (2016)
   PUBMED   27107014
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2254)
  AUTHORS   Conaway,J.W., Florens,L., Sato,S., Tomomori-Sato,C., Parmely,T.J.,
            Yao,T., Swanson,S.K., Banks,C.A., Washburn,M.P. and Conaway,R.C.
  TITLE     The mammalian Mediator complex
  JOURNAL   FEBS Lett 579 (4), 904-908 (2005)
   PUBMED   15680972
  REMARK    Review article
REFERENCE   7  (bases 1 to 2254)
  AUTHORS   Mittler,G., Kremmer,E., Timmers,H.T. and Meisterernst,M.
  TITLE     Novel critical role of a human Mediator complex for basal RNA
            polymerase II transcription
  JOURNAL   EMBO Rep 2 (9), 808-813 (2001)
   PUBMED   11559591
REFERENCE   8  (bases 1 to 2254)
  AUTHORS   Malik,S., Gu,W., Wu,W., Qin,J. and Roeder,R.G.
  TITLE     The USA-derived transcriptional coactivator PC2 is a submodule of
            TRAP/SMCC and acts synergistically with other PCs
  JOURNAL   Mol Cell 5 (4), 753-760 (2000)
   PUBMED   10882111
REFERENCE   9  (bases 1 to 2254)
  AUTHORS   Naar,A.M., Beaurang,P.A., Zhou,S., Abraham,S., Solomon,W. and
            Tjian,R.
  TITLE     Composite co-activator ARC mediates chromatin-directed
            transcriptional activation
  JOURNAL   Nature 398 (6730), 828-832 (1999)
   PUBMED   10235267
REFERENCE   10 (bases 1 to 2254)
  AUTHORS   Rachez,C., Lemon,B.D., Suldan,Z., Bromleigh,V., Gamble,M.,
            Naar,A.M., Erdjument-Bromage,H., Tempst,P. and Freedman,L.P.
  TITLE     Ligand-dependent transcription activation by nuclear receptors
            requires the DRIP complex
  JOURNAL   Nature 398 (6730), 824-828 (1999)
   PUBMED   10235266
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK001934.1, AL158196.24 and
            AI613470.1.
            
            On May 17, 2019 this sequence version replaced NM_014166.3.
            
            Summary: This gene encodes a component of the Mediator complex. The
            Mediator complex interacts with DNA-binding gene-specific
            transcription factors to modulate transcription by RNA polymerase
            II. Alternatively spliced transcript variants encoding multiple
            isoforms have been observed for this gene. [provided by RefSeq, Jul
            2012].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.180219.1,
                                           SRR1660807.147776.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000258648.7/ ENSP00000258648.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1157              AK001934.1         1-1157
            1158-1158           AL158196.24        61545-61545         c
            1159-2022           AK001934.1         1159-2022
            2023-2250           AI613470.1         56-283              c
            2251-2254           AL158196.24        60449-60452         c
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q14.2"
     gene            1..2254
                     /gene="MED4"
                     /gene_synonym="ARC36; DRIP36; HSPC126; TRAP36; VDRIP"
                     /note="mediator complex subunit 4"
                     /db_xref="GeneID:29079"
                     /db_xref="HGNC:HGNC:17903"
                     /db_xref="MIM:605718"
     exon            1..151
                     /gene="MED4"
                     /gene_synonym="ARC36; DRIP36; HSPC126; TRAP36; VDRIP"
                     /inference="alignment:Splign:2.1.0"
     CDS             27..839
                     /gene="MED4"
                     /gene_synonym="ARC36; DRIP36; HSPC126; TRAP36; VDRIP"
                     /note="isoform 1 is encoded by transcript variant 1;
                     mediator of RNA polymerase II transcription subunit 4;
                     activator-recruited cofactor 36 kDa component; vitamin D3
                     receptor-interacting protein complex 36 kDa component;
                     mediator, 34-kD subunit, homolog; vitamin D
                     receptor-interacting protein, 36-kD"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 4 isoform 1"
                     /protein_id="NP_054885.1"
                     /db_xref="CCDS:CCDS9408.1"
                     /db_xref="GeneID:29079"
                     /db_xref="HGNC:HGNC:17903"
                     /db_xref="MIM:605718"
                     /translation="MAASSSGEKEKERLGGGLGVAGGNSTRERLLSALEDLEVLSREL
                     IEMLAISRNQKLLQAGEENQVLELLIHRDGEFQELMKLALNQGKIHHEMQVLEKEVEK
                     RDSDIQQLQKQLKEAEQILATAVYQAKEKLKSIEKARKGAISSEEIIKYAHRISASNA
                     VCAPLTWVPGDPRRPYPTDLEMRSGLLGQMNNPSTNGVNGHLPGDALAAGRLPDVLAP
                     QYPWQSNDMSMNMLPPNHSSDFLLEPPGHNKENEDDVEIMSTDSSSSSSESD"
     exon            152..218
                     /gene="MED4"
                     /gene_synonym="ARC36; DRIP36; HSPC126; TRAP36; VDRIP"
                     /inference="alignment:Splign:2.1.0"
     exon            219..389
                     /gene="MED4"
                     /gene_synonym="ARC36; DRIP36; HSPC126; TRAP36; VDRIP"
                     /inference="alignment:Splign:2.1.0"
     exon            390..447
                     /gene="MED4"
                     /gene_synonym="ARC36; DRIP36; HSPC126; TRAP36; VDRIP"
                     /inference="alignment:Splign:2.1.0"
     exon            448..534
                     /gene="MED4"
                     /gene_synonym="ARC36; DRIP36; HSPC126; TRAP36; VDRIP"
                     /inference="alignment:Splign:2.1.0"
     exon            535..666
                     /gene="MED4"
                     /gene_synonym="ARC36; DRIP36; HSPC126; TRAP36; VDRIP"
                     /inference="alignment:Splign:2.1.0"
     exon            667..2254
                     /gene="MED4"
                     /gene_synonym="ARC36; DRIP36; HSPC126; TRAP36; VDRIP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1378..1383
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED4"
                     /gene_synonym="ARC36; DRIP36; HSPC126; TRAP36; VDRIP"
                     /note="hexamer: AATAAA"
     polyA_site      1398
                     /gene="MED4"
                     /gene_synonym="ARC36; DRIP36; HSPC126; TRAP36; VDRIP"
                     /note="major polyA site"
     regulatory      1842..1847
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED4"
                     /gene_synonym="ARC36; DRIP36; HSPC126; TRAP36; VDRIP"
                     /note="hexamer: AATAAA"
     polyA_site      1879
                     /gene="MED4"
                     /gene_synonym="ARC36; DRIP36; HSPC126; TRAP36; VDRIP"
     regulatory      1995..2000
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED4"
                     /gene_synonym="ARC36; DRIP36; HSPC126; TRAP36; VDRIP"
                     /note="hexamer: AATGAA"
     polyA_site      2023
                     /gene="MED4"
                     /gene_synonym="ARC36; DRIP36; HSPC126; TRAP36; VDRIP"
     polyA_site      2254
                     /gene="MED4"
                     /gene_synonym="ARC36; DRIP36; HSPC126; TRAP36; VDRIP"
ORIGIN      
        1 gcgccggtgg cgggactctg gggaaaatgg ctgcgtcttc gagtggtgag aaggagaagg
       61 agcggctggg aggcggtttg ggagtggcgg gtggtaacag cacacgagag cggctgctgt
      121 ctgcgcttga ggacttggag gtcctgtcta gggaacttat agaaatgctg gcaatttcaa
      181 gaaaccaaaa gttgttacag gctggagagg aaaaccaggt cctggagttg ttaattcacc
      241 gagatgggga atttcaagaa ctaatgaaat tggcacttaa tcagggaaaa attcatcatg
      301 aaatgcaagt tttagaaaaa gaagtagaga agagagacag tgatattcag cagctacaaa
      361 aacagctaaa ggaagcagaa caaatactgg caacagctgt ttaccaagcg aaggagaaac
      421 tcaagtcaat agaaaaagca agaaaaggtg ctatctcctc tgaagaaata attaagtatg
      481 cacataggat cagtgcaagt aatgctgtat gtgctccact gacctgggtt ccaggggacc
      541 cccggagacc ctacccaact gatttagaga tgagaagtgg gttactgggt cagatgaaca
      601 atccttccac taatggcgtg aatggccatt taccaggaga tgcacttgca gcaggaagat
      661 tgccagatgt ccttgctcca cagtatccat ggcagtcaaa tgacatgtcg atgaatatgt
      721 taccaccaaa tcatagtagt gactttttgt tggaacctcc tgggcataat aaagaaaatg
      781 aagatgatgt agagattatg tcaacggact cctcaagcag tagtagtgag tctgattgaa
      841 aaaccttaaa agacaatata cagaattgaa tactgtagaa ttctgtttct ttaacagtag
      901 cagggaaatg taaactacag gtgacaaaaa atacccaggt aaacactggc tttggtagaa
      961 ttgtgcagtc attaaaagtc aaaatttttt gactttcttt ttaaagccaa agaccatagt
     1021 tttagtttta agccactagg tagatattta ggggaatagt caaaatttac tgttgaaaaa
     1081 gcagttgcta tgtgctttct taccctgttc tgttccagtt ttgctggatt tgtacatagc
     1141 cattctagaa atagagttga gggaaattat ccatatacat atcactaata ggcttcttgg
     1201 aattatttag aaaagcattt ttaaactggc agtggatgac tgaataggca tcatatttct
     1261 ttttgtgtgt catttaaaag taacaaaaac tgccatttga cagtaaaggc tcttggcttc
     1321 tgttggaggc atgggaaatt gtctcaattt gtacagtttg taattgtaat ttttgtaaat
     1381 aaatttgttt gtacattgta tgcttttttt ttagtttaaa tatactgtca gtgtaggtag
     1441 gaaattattt ctgtaaatcc agttgcattt tataatactt gagtgactaa ctgaattgaa
     1501 acctattaca attaataggg ttctcaaagg cctattccta ttagaagaaa taattttttt
     1561 taataattga aaaaatttac cacagccttg tctgatttta agaatagatt gtgtgaagat
     1621 ttagaatgga aagcaaccat ttcttccctt caccttcctc ttcttacagt tccccaggag
     1681 tcatttttaa ccacttttaa tttctgttgg taacctctgt gtttaggtaa tatgcttata
     1741 caactatttc ttgatgtatc agttttagac aatgtatctt ctgatttcaa aggtaataca
     1801 acatttaatg tgaaacattt agtgaataca gtaaaggaga aaataaaatt gttagcattc
     1861 tacccagaga taatatcaat tcatattgta gtgtagattt ttttaaagtt cacatacact
     1921 tttttgtaaa atgagaataa tgtttatttc ctgagatact gctgtatttt ttaattgcta
     1981 atctgtattc ttcaaatgaa ctgagaattc catttttcca tcattttttc cttttgatta
     2041 ttttcgatct ctagaaaaat tggaaaatag gagattaatc ttatattttc accatctaga
     2101 attaaggaca tacaatgttt gaatgtcttt ccctccaggg ttatttttag gaatggacta
     2161 acacaatatc taattttaac cttgccttgt ccgtatacta ggaatattga tccatgtgta
     2221 tacattcttg aaagatttac tttaatggct aaaa
//
