LOCUS       NM_016060               1629 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens mediator complex subunit 31 (MED31), mRNA.
ACCESSION   NM_016060
VERSION     NM_016060.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 1629)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 1629)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 1629)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   6  (bases 1 to 1629)
  AUTHORS   Sato,S., Tomomori-Sato,C., Banks,C.A., Sorokina,I., Parmely,T.J.,
            Kong,S.E., Jin,J., Cai,Y., Lane,W.S., Brower,C.S., Conaway,R.C. and
            Conaway,J.W.
  TITLE     Identification of mammalian Mediator subunits with similarities to
            yeast Mediator subunits Srb5, Srb6, Med11, and Rox3
  JOURNAL   J Biol Chem 278 (17), 15123-15127 (2003)
   PUBMED   12584197
REFERENCE   7  (bases 1 to 1629)
  AUTHORS   Brower,C.S., Sato,S., Tomomori-Sato,C., Kamura,T., Pause,A.,
            Stearman,R., Klausner,R.D., Malik,S., Lane,W.S., Sorokina,I.,
            Roeder,R.G., Conaway,J.W. and Conaway,R.C.
  TITLE     Mammalian mediator subunit mMED8 is an Elongin BC-interacting
            protein that can assemble with Cul2 and Rbx1 to reconstitute a
            ubiquitin ligase
  JOURNAL   Proc Natl Acad Sci U S A 99 (16), 10353-10358 (2002)
   PUBMED   12149480
REFERENCE   8  (bases 1 to 1629)
  AUTHORS   Suzuki,H., Fukunishi,Y., Kagawa,I., Saito,R., Oda,H., Endo,T.,
            Kondo,S., Bono,H., Okazaki,Y. and Hayashizaki,Y.
  TITLE     Protein-protein interaction panel using mouse full-length cDNAs
  JOURNAL   Genome Res 11 (10), 1758-1765 (2001)
   PUBMED   11591653
REFERENCE   9  (bases 1 to 1629)
  AUTHORS   Mittler,G., Kremmer,E., Timmers,H.T. and Meisterernst,M.
  TITLE     Novel critical role of a human Mediator complex for basal RNA
            polymerase II transcription
  JOURNAL   EMBO Rep 2 (9), 808-813 (2001)
   PUBMED   11559591
REFERENCE   10 (bases 1 to 1629)
  AUTHORS   Gu,W., Malik,S., Ito,M., Yuan,C.X., Fondell,J.D., Zhang,X.,
            Martinez,E., Qin,J. and Roeder,R.G.
  TITLE     A novel human SRB/MED-containing cofactor complex, SMCC, involved
            in transcription regulation
  JOURNAL   Mol Cell 3 (1), 97-108 (1999)
   PUBMED   10024883
  REMARK    Erratum:[Mol Cell 1999 Apr;3(4):following 541]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF151883.1 and AC004706.1.
            
            On Nov 23, 2018 this sequence version replaced NM_016060.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DA511624.1, SRR1660807.55598.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2146411, SAMEA2467147
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000225728.8/ ENSP00000225728.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1195              AF151883.1         2-1196
            1196-1627           AC004706.1         19021-19452         c
            1628-1629           AC004706.1         19019-19020         c
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p13.1"
     gene            1..1629
                     /gene="MED31"
                     /gene_synonym="3110004H13Rik; CGI-125; SEP10; Soh1"
                     /note="mediator complex subunit 31"
                     /db_xref="GeneID:51003"
                     /db_xref="HGNC:HGNC:24260"
                     /db_xref="MIM:620492"
     exon            1..105
                     /gene="MED31"
                     /gene_synonym="3110004H13Rik; CGI-125; SEP10; Soh1"
                     /inference="alignment:Splign:2.1.0"
     CDS             78..473
                     /gene="MED31"
                     /gene_synonym="3110004H13Rik; CGI-125; SEP10; Soh1"
                     /note="hSOH1; mediator complex subunit SOH1; mediator of
                     RNA polymerase II transcription, subunit 31 homolog"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 31"
                     /protein_id="NP_057144.1"
                     /db_xref="CCDS:CCDS11078.1"
                     /db_xref="GeneID:51003"
                     /db_xref="HGNC:HGNC:24260"
                     /db_xref="MIM:620492"
                     /translation="MAAAVAMETDDAGNRLRFQLELEFVQCLANPNYLNFLAQRGYFK
                     DKAFVNYLKYLLYWKDPEYAKYLKYPQCLHMLELLQYEHFRKELVNAQCAKFIDEQQI
                     LHWQHYSRKRMRLQQALAEQQQQNNTSGK"
     exon            106..183
                     /gene="MED31"
                     /gene_synonym="3110004H13Rik; CGI-125; SEP10; Soh1"
                     /inference="alignment:Splign:2.1.0"
     exon            184..280
                     /gene="MED31"
                     /gene_synonym="3110004H13Rik; CGI-125; SEP10; Soh1"
                     /inference="alignment:Splign:2.1.0"
     exon            281..1629
                     /gene="MED31"
                     /gene_synonym="3110004H13Rik; CGI-125; SEP10; Soh1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      626..631
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED31"
                     /gene_synonym="3110004H13Rik; CGI-125; SEP10; Soh1"
                     /note="hexamer: AATAGA"
     polyA_site      651
                     /gene="MED31"
                     /gene_synonym="3110004H13Rik; CGI-125; SEP10; Soh1"
                     /note="major polyA site"
     regulatory      1603..1608
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED31"
                     /gene_synonym="3110004H13Rik; CGI-125; SEP10; Soh1"
                     /note="hexamer: AATAAA"
     polyA_site      1629
                     /gene="MED31"
                     /gene_synonym="3110004H13Rik; CGI-125; SEP10; Soh1"
ORIGIN      
        1 gtggtttccg gaacttcgcc cgcgtctctg ggcttttgct ctgtcaggct ggtggcgttt
       61 tggtgtcttc gtttgttatg gccgctgctg tcgctatgga gacagatgat gctggaaatc
      121 gacttcggtt tcagttggag ttggaatttg tgcaatgttt agccaaccca aattacctta
      181 attttcttgc ccaaagaggt tacttcaaag acaaagcttt tgttaattat cttaaatact
      241 tgctttactg gaaagaccca gaatatgcca agtatctaaa gtaccctcag tgtttacaca
      301 tgttagagct gctccaatat gaacacttcc gaaaggagct ggtgaatgct cagtgtgcga
      361 aatttattga tgaacagcag attctacatt ggcagcacta ttcccggaag cggatgcgcc
      421 ttcagcaagc cttggcagag cagcaacagc aaaataacac atcgggaaaa tgaaaaactg
      481 gatacaaacg aggcacttaa tacatgtata taatgtattt cttttgtaca gtgaagacaa
      541 aaaaaatgaa aactcttcct atctaccttt attatggtag cccctagaac ctttagtgtg
      601 ctttttcatc caacttttta ttgttaatag actatttctg ttaaacctga attgttcttt
      661 gatttccctg tggatttgta aggtgttttt cttttttacg catttagcat aatcagctga
      721 ttaagaatgg aaaagttagg tgctttagac caaataaggc actggggcat ggggtagggg
      781 tgggccacca agtatactga attctaaggc tagctgttcc tgacatatag tagggcaaga
      841 ccatctcctg gaaccttact gtctcagatc aactactcta ccactctact ctaagatgta
      901 gtgagagtag cggagtcact gacctcttta aatcatagga caggaactgc ataaaggttc
      961 ttgaattctg caggacagga aataactgta ttacctgctt tgcaaggtgg ctttaagaat
     1021 cactgaatca ctgtggcctc acttgaccta gtcatggcag gacaactagt ggttagttac
     1081 taactggact cttgacttta gaacaaacat gaggagcctc aaatacttgt gttcatttgg
     1141 gaaagctacc attgatttgg gaggtaccac aaaccaggtg ctatgttaag tgcttccttg
     1201 aattctcatt ttattctaag aaccttgttt atagaagaga aaactgaggt ttggacgggg
     1261 tgagtaactt gcctgtggtt acacagctag taagtggtag agtcagtgtg actgactccc
     1321 agcttggagt tcttcccaga actgggttat agagtctttc cggccgctct ccgccacctg
     1381 cctcatccca agtaaagcac attgagacag ctgcaaaaca aactgcttct agtgctcagg
     1441 agtctgtttt ttattaaaca aaacaaaact gcctgaatta agcagaggct agattagagg
     1501 tcatgtgctt tccttcagtg gtgaggttag gttctacatt ccaaaatcaa agaaggggtt
     1561 tcaggaatat catcagttgc tttgcagatg gagataactt aaaataaaag atactgatct
     1621 tttgagaga
//
