LOCUS       NM_080651                985 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens mediator complex subunit 30 (MED30), transcript
            variant 1, mRNA.
ACCESSION   NM_080651
VERSION     NM_080651.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 985)
  AUTHORS   Shukla,A., Srivastava,S., Darokar,J. and Kulshreshtha,R.
  TITLE     HIF1alpha and p53 Regulated MED30, a Mediator Complex Subunit, is
            Involved in Regulation of Glioblastoma Pathogenesis and
            Temozolomide Resistance
  JOURNAL   Cell Mol Neurobiol 41 (7), 1521-1535 (2021)
   PUBMED   32705436
  REMARK    GeneRIF: HIF1alpha and p53 Regulated MED30, a Mediator Complex
            Subunit, is Involved in Regulation of Glioblastoma Pathogenesis and
            Temozolomide Resistance.
REFERENCE   3  (bases 1 to 985)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 985)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 985)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 985)
  AUTHORS   Suzuki,H., Fukunishi,Y., Kagawa,I., Saito,R., Oda,H., Endo,T.,
            Kondo,S., Bono,H., Okazaki,Y. and Hayashizaki,Y.
  TITLE     Protein-protein interaction panel using mouse full-length cDNAs
  JOURNAL   Genome Res 11 (10), 1758-1765 (2001)
   PUBMED   11591653
REFERENCE   7  (bases 1 to 985)
  AUTHORS   Mittler,G., Kremmer,E., Timmers,H.T. and Meisterernst,M.
  TITLE     Novel critical role of a human Mediator complex for basal RNA
            polymerase II transcription
  JOURNAL   EMBO Rep 2 (9), 808-813 (2001)
   PUBMED   11559591
REFERENCE   8  (bases 1 to 985)
  AUTHORS   Naar,A.M., Beaurang,P.A., Zhou,S., Abraham,S., Solomon,W. and
            Tjian,R.
  TITLE     Composite co-activator ARC mediates chromatin-directed
            transcriptional activation
  JOURNAL   Nature 398 (6730), 828-832 (1999)
   PUBMED   10235267
REFERENCE   9  (bases 1 to 985)
  AUTHORS   Rachez,C., Lemon,B.D., Suldan,Z., Bromleigh,V., Gamble,M.,
            Naar,A.M., Erdjument-Bromage,H., Tempst,P. and Freedman,L.P.
  TITLE     Ligand-dependent transcription activation by nuclear receptors
            requires the DRIP complex
  JOURNAL   Nature 398 (6730), 824-828 (1999)
   PUBMED   10235266
REFERENCE   10 (bases 1 to 985)
  AUTHORS   Ito,M., Yuan,C.X., Malik,S., Gu,W., Fondell,J.D., Yamamura,S.,
            Fu,Z.Y., Zhang,X., Qin,J. and Roeder,R.G.
  TITLE     Identity between TRAP and SMCC complexes indicates novel pathways
            for the function of nuclear receptors and diverse mammalian
            activators
  JOURNAL   Mol Cell 3 (3), 361-370 (1999)
   PUBMED   10198638
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC024329.9, AY083305.1, AC087361.5 and AA812233.1.
            
            On Nov 22, 2018 this sequence version replaced NM_080651.3.
            
            Summary: The multiprotein TRAP/Mediator complex facilitates gene
            expression through a wide variety of transcriptional activators.
            MED30 is a component of this complex that appears to be metazoan
            specific (Baek et al., 2002 [PubMed 11909976]).[supplied by OMIM,
            Nov 2010].
            
            Transcript Variant: This variant (1) represents the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189664.43915.1,
                                           SRR5189655.109161.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000297347.7/ ENSP00000297347.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-164               AC024329.9         10235-10398         c
            165-701             AY083305.1         1-537
            702-865             AC087361.5         48190-48353
            866-985             AA812233.1         10-129              c
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q24.11"
     gene            1..985
                     /gene="MED30"
                     /gene_synonym="MED30S; THRAP6; TRAP25"
                     /note="mediator complex subunit 30"
                     /db_xref="GeneID:90390"
                     /db_xref="HGNC:HGNC:23032"
                     /db_xref="MIM:610237"
     exon            1..341
                     /gene="MED30"
                     /gene_synonym="MED30S; THRAP6; TRAP25"
                     /inference="alignment:Splign:2.1.0"
     CDS             165..701
                     /gene="MED30"
                     /gene_synonym="MED30S; THRAP6; TRAP25"
                     /note="isoform 1 is encoded by transcript variant 1;
                     mediator of RNA polymerase II transcription subunit 30;
                     putative mediator of RNA polymerase II transcription
                     subunit 30; TRAP/Mediator complex component TRAP25;
                     thyroid hormone receptor-associated protein 6; thyroid
                     hormone receptor-associated protein complex 25 kDa
                     component"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 30 isoform 1"
                     /protein_id="NP_542382.1"
                     /db_xref="CCDS:CCDS6323.1"
                     /db_xref="GeneID:90390"
                     /db_xref="HGNC:HGNC:23032"
                     /db_xref="MIM:610237"
                     /translation="MSTPPLAASGMAPGPFAGPQAQQAAREVNTASLCRIGQETVQDI
                     VYRTMEIFQLLRNMQLPNGVTYHTGTYQDRLTKLQDNLRQLSVLFRKLRLVYDKCNEN
                     CGGMDPIPVEQLIPYVEEDGSKNDDRAGPPRFASEERREIAEVNKKLKQKNQQLKQIM
                     DQLRNLIWDINAMLAMRN"
     exon            342..500
                     /gene="MED30"
                     /gene_synonym="MED30S; THRAP6; TRAP25"
                     /inference="alignment:Splign:2.1.0"
     exon            501..605
                     /gene="MED30"
                     /gene_synonym="MED30S; THRAP6; TRAP25"
                     /inference="alignment:Splign:2.1.0"
     exon            606..985
                     /gene="MED30"
                     /gene_synonym="MED30S; THRAP6; TRAP25"
                     /inference="alignment:Splign:2.1.0"
     regulatory      960..965
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED30"
                     /gene_synonym="MED30S; THRAP6; TRAP25"
                     /note="hexamer: AATAAA"
     polyA_site      985
                     /gene="MED30"
                     /gene_synonym="MED30S; THRAP6; TRAP25"
                     /note="major polyA site"
ORIGIN      
        1 gtgttacgtc acagtgggcg gaagtcgcgg ccgctgtttt gaaatcgggc cgcggggggt
       61 ctctcaagct ggttccaacg ctgaggcccc acagcctccc aattccgggc agacccctga
      121 cacctgctgt ctggcccctt ccggcctgaa gctgcagccg cgccatgtcc acccctccgt
      181 tggccgcgtc ggggatggcg cccgggccct tcgccgggcc ccaggctcag caggccgccc
      241 gggaagtcaa cacggcgtcg ctgtgccgca tcgggcagga gacagtgcag gacatcgtgt
      301 accgcaccat ggagatcttc cagctcctga ggaacatgca gctgccaaat ggtgtcactt
      361 accacactgg aacatatcaa gaccggttaa caaagctaca ggataatctt cgccaacttt
      421 cagttctctt caggaagctg agattggtat atgacaaatg caatgaaaac tgtggtggga
      481 tggatcccat tccagtcgag caacttattc catatgtgga agaagatggc tcaaagaatg
      541 atgatcgggc tggcccacct cgttttgcta gtgaagagag gcgagaaatt gctgaagtaa
      601 ataaaaaact caaacagaag aatcaacagc tgaaacaaat tatggatcaa ttacgaaatc
      661 tcatctggga tataaatgcc atgttggcaa tgaggaacta agctgatatt taaatttcct
      721 gctttacaca tgttatacca ttgttttttc cctcaagtat tttttccctg tgaagaagat
      781 tatttatctg cttttatttt agtcactaaa actaaagttt ttatttttac attgtgattt
      841 ttacattaaa atattaactt tttttaatgc tattttatga aagattattg taataaactt
      901 tgatggggtt tgtattttgg ttaatcttca tgaattgaat aattgttttt ttaaagcaaa
      961 ataaagtttt ttaaataaat gttaa
//
