LOCUS       NM_017592               3590 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens mediator complex subunit 29 (MED29), transcript
            variant 1, mRNA.
ACCESSION   NM_017592 XM_290829
VERSION     NM_017592.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Huang,S., Zhang,J., Qiao,Y., Pathak,J.L., Zou,R., Piao,Z., Xie,S.,
            Liang,J. and Ouyang,K.
  TITLE     CHRDL1 inhibits OSCC metastasis via MAPK signaling-mediated
            inhibition of MED29
  JOURNAL   Mol Med 30 (1), 187 (2024)
   PUBMED   39462350
  REMARK    GeneRIF: CHRDL1 inhibits OSCC metastasis via MAPK
            signaling-mediated inhibition of MED29.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 3590)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 3590)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 3590)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   6  (bases 1 to 3590)
  AUTHORS   Wang,Y., Li,Y., Zeng,W., Zhu,C., Xiao,J., Yuan,W., Wang,Y., Cai,Z.,
            Zhou,J., Liu,M. and Wu,X.
  TITLE     IXL, a new subunit of the mammalian Mediator complex, functions as
            a transcriptional suppressor
  JOURNAL   Biochem Biophys Res Commun 325 (4), 1330-1338 (2004)
   PUBMED   15555573
  REMARK    GeneRIF: Overexpression of IXL in COS-7 cells inhibits the
            transcriptional activities of SRE and AP-1, suggesting that the IXL
            protein may act as a transcriptional suppressor in
            mitogen-activated protein kinase signaling pathway to mediate
            cellular functions.
REFERENCE   7  (bases 1 to 3590)
  AUTHORS   Sato,S., Tomomori-Sato,C., Parmely,T.J., Florens,L., Zybailov,B.,
            Swanson,S.K., Banks,C.A., Jin,J., Cai,Y., Washburn,M.P.,
            Conaway,J.W. and Conaway,R.C.
  TITLE     A set of consensus mammalian mediator subunits identified by
            multidimensional protein identification technology
  JOURNAL   Mol Cell 14 (5), 685-691 (2004)
   PUBMED   15175163
  REMARK    GeneRIF: IXL is a subunit of the mammalian Mediator complex.
REFERENCE   8  (bases 1 to 3590)
  AUTHORS   Tomomori-Sato,C., Sato,S., Parmely,T.J., Banks,C.A., Sorokina,I.,
            Florens,L., Zybailov,B., Washburn,M.P., Brower,C.S., Conaway,R.C.
            and Conaway,J.W.
  TITLE     A mammalian mediator subunit that shares properties with
            Saccharomyces cerevisiae mediator subunit Cse2
  JOURNAL   J Biol Chem 279 (7), 5846-5851 (2004)
   PUBMED   14638676
REFERENCE   9  (bases 1 to 3590)
  AUTHORS   Sato,S., Tomomori-Sato,C., Banks,C.A., Parmely,T.J., Sorokina,I.,
            Brower,C.S., Conaway,R.C. and Conaway,J.W.
  TITLE     A mammalian homolog of Drosophila melanogaster transcriptional
            coactivator intersex is a subunit of the mammalian Mediator complex
  JOURNAL   J Biol Chem 278 (50), 49671-49674 (2003)
   PUBMED   14576168
  REMARK    GeneRIF: A mammalian homolog of Drosophila melanogaster
            transcriptional coactivator intersex is a subunit of the mammalian
            Mediator complex
            GeneRIF: IXL is a subunit of the mammalian Mediator complex.
REFERENCE   10 (bases 1 to 3590)
  AUTHORS   Mittler,G., Kremmer,E., Timmers,H.T. and Meisterernst,M.
  TITLE     Novel critical role of a human Mediator complex for basal RNA
            polymerase II transcription
  JOURNAL   EMBO Rep 2 (9), 808-813 (2001)
   PUBMED   11559591
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AY729650.1, AK124629.1, BQ271530.1 and AC011500.7.
            
            On May 17, 2019 this sequence version replaced NM_017592.3.
            
            Summary: MED29 is a subunit of the Mediator complex, a multiprotein
            coactivator of RNA transcription that interacts with DNA-bound
            transcriptional activators, RNA polymerase II (see MIM 180660), and
            general initiation factors (Sato et al., 2003 [PubMed
            14576168]).[supplied by OMIM, Aug 2009].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.81167.1, AY729650.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000315588.11/ ENSP00000314343.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2959              AY729650.1         56-3014
            2960-2960           AK124629.1         2898-2898
            2961-3469           AY729650.1         3016-3524
            3470-3470           AK124629.1         3408-3408
            3471-3512           AY729650.1         3526-3567
            3513-3586           BQ271530.1         9-82                c
            3587-3590           AC011500.7         5462-5465
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.2"
     gene            1..3590
                     /gene="MED29"
                     /gene_synonym="IXL; MED2"
                     /note="mediator complex subunit 29"
                     /db_xref="GeneID:55588"
                     /db_xref="HGNC:HGNC:23074"
                     /db_xref="MIM:612914"
     exon            1..261
                     /gene="MED29"
                     /gene_synonym="IXL; MED2"
                     /inference="alignment:Splign:2.1.0"
     CDS             46..648
                     /gene="MED29"
                     /gene_synonym="IXL; MED2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     mediator of RNA polymerase II transcription subunit 29;
                     intersex-like protein"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 29 isoform 1"
                     /protein_id="NP_060062.2"
                     /db_xref="CCDS:CCDS33021.2"
                     /db_xref="GeneID:55588"
                     /db_xref="HGNC:HGNC:23074"
                     /db_xref="MIM:612914"
                     /translation="MAASQQQASAASSAAGVSGPSSAGGPGPQQQPQPPAQLVGPAQS
                     GLLQQQQQDFDPVQRYKMLIPQLKESLQTLMKVAAQNLIQNTNIDNGQKSSDGPIQRF
                     DKCLEEFYALCDQLELCLRLAHECLSQSCDSAKHSPTLVPTATKPDAVQPDSLPYPQY
                     LAVIKAQISCAKDIHTALLDCANKVTGKTPAPPAGPGGTL"
     exon            262..320
                     /gene="MED29"
                     /gene_synonym="IXL; MED2"
                     /inference="alignment:Splign:2.1.0"
     exon            321..405
                     /gene="MED29"
                     /gene_synonym="IXL; MED2"
                     /inference="alignment:Splign:2.1.0"
     exon            406..3590
                     /gene="MED29"
                     /gene_synonym="IXL; MED2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      702..707
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED29"
                     /gene_synonym="IXL; MED2"
                     /note="hexamer: AATGAA"
     polyA_site      725
                     /gene="MED29"
                     /gene_synonym="IXL; MED2"
                     /note="major polyA site"
     regulatory      3492..3497
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED29"
                     /gene_synonym="IXL; MED2"
                     /note="hexamer: ATTAAA"
     polyA_site      3512
                     /gene="MED29"
                     /gene_synonym="IXL; MED2"
                     /note="major polyA site"
     regulatory      3571..3576
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED29"
                     /gene_synonym="IXL; MED2"
                     /note="hexamer: ATTAAA"
     polyA_site      3590
                     /gene="MED29"
                     /gene_synonym="IXL; MED2"
ORIGIN      
        1 gagagacgca gtcgtaacgc acttccggcg gtctacgcga ggaagatggc tgcatcccag
       61 cagcaagctt cagcggcttc ctcagctgct ggtgtatcgg gtcctagttc ggctggcggc
      121 ccgggtcccc agcagcagcc gcaaccgcca gcacaactgg tgggccctgc ccagagcggc
      181 ctcctgcagc aacagcaaca ggacttcgat cctgtgcagc gttataagat gctcatcccg
      241 cagctgaagg agagtctaca gaccttgatg aaggttgcgg cccaaaactt gattcagaac
      301 actaacatcg acaatggaca aaagagcagt gatggaccca tacagcgctt tgacaagtgc
      361 ctggaagagt tctatgcact ctgtgaccag ctggagctgt gcctgcgcct ggcgcatgag
      421 tgcctgtcac agagttgtga cagtgccaag cactctccaa cgttggtgcc cacagccacc
      481 aagcccgacg cagtgcagcc tgacagcctc ccctacccac agtacctggc ggtcatcaaa
      541 gcccagattt cctgtgccaa ggacattcac accgccctgc tggactgtgc caacaaggtc
      601 acgggcaaga cacccgcacc acctgctggc cctgggggca ctctgtgaag tgggggacag
      661 ggagtggggc aggcagtggt tggtgggtgg tgtgcaaagg gaatgaagag cgtcctgggc
      721 ctaaacacag cagcctcctc tcttcctgcc tgagcaccgc agcgggagcc agcagggggc
      781 agcagaggcc aacagggagc tcgcaggccg ggcccctgcg tccctgcccc ttcttcctgc
      841 tccccctcct agcctagggt agactttgaa ctgtgtgtgt tgatgacttc tctgttccac
      901 aggccctccc ccattcttgc ctgggtgtgg agccctggct gtcccctctc cctcagtcct
      961 tcctgactgt ctccagctgg gaggtggtct ctgtgtgcca ctcctctgtg tctctattac
     1021 agttgtgtct ctctcatcct gtctcttttt cccttgtttc tctgttcctg ttaatgtgtt
     1081 tctccccatg gtcctatttc tctcactctg acctctctct cttagtcccc tttagctgtc
     1141 ttctatcccc agctcctaac tgggactctg tgtctatgca gggggccagc acccctgggt
     1201 tatctggggc taagggaagg gacttcattt ccaggggcca cagccaagcc cagagtcccc
     1261 cagcggctcg catgtcagcc cagaccccag ggtccttggc ctaggagagg agcagtggag
     1321 gggcccaggc tctgagctcc acaggtctga gcagggagca actcaggccc ccacccaagc
     1381 ctgcgtcagc ggaacttgag tgaggggcgt tgtgcaattt gtggcaaggc tggcccagct
     1441 ggatgcctgg gtcccagtat ttttagcccc aaaggagaag tgaaaaggcc ccagccgggg
     1501 tgaatcatca gtcctgggga agaacccagg cgcctgagcc ccagctccgg gaagcaggca
     1561 ctggggaggg ggcttcaagg agggagtgcc ccctcagact ccctgcttcc ctggaagctt
     1621 caggaagctc agcctcagcc ttcaggcctg agcaagtgca gggcggagct accagcccag
     1681 gctcagatgt tggggtgtga aagcctcaag tgactcagcc tggttggaga actgccccac
     1741 ccagtatctt ctgtgccatg gttcccacat tcgcactcca tggcctcctg tcctggaccc
     1801 cacgtctgca aggaaaccct aggaccatgg atacctctgt gattcacgct gagcccaagt
     1861 ccccacactg gaaaactggg aaatggccag ctgtgtgtcc caggaaattc ctccccttat
     1921 tcttccttga agtgcccgag catgtagggc aagaaggaag gctgaagcgc tgtccctagg
     1981 aggaatttct ccttcagggg agcctcagtt ttgcccattt atctaattga atcagttttt
     2041 tacccaatcc cccgattttg taggataatc tcccttatct aaagtcaact gattatggac
     2101 tttaatcaca tctacaaaac acttccatgg cgacagctag atgagtgttt gaataactgg
     2161 gactgtagcc cgtccaagtt gacacataaa actgaccatc gggccggggg cggtggctca
     2221 cgcctgtaat cccaacactt tgggagcccg aggcgggcgg atcacaaggt caggagttcg
     2281 agaccagcct ggccaacacg gtgaaacccc gactctacta aaaatacaaa aaattagccg
     2341 ggtgtggtgg cacacacctg tagtcccagc tactcgggag gctgaggcag gagaatcgtt
     2401 tgaacctggg aggcagaggt tgcagtgagc caagatcaca ctattgcact ccagcctggg
     2461 cgacagggca agactctgtc tcaaaaaaaa taaaaaactg accatctagt ccttgtcatc
     2521 tgggcaccct cacacatctc cttaaccaca cttaatctcc aaataagtac gataacatag
     2581 tcatagtccc acccaacatg atgcagttat cttgcataca actgaagaca actaaccctt
     2641 tccccaacag agcccaccag cagtggtgga gatgtcggtc catgagcgca cacacaagac
     2701 tgagggactg tcggccctcc caggtggtgt caacacaaca tcacacacag gtgggggggc
     2761 ctgatagccc agcacccatg atacagggcc taccaatgct taaaaccaca cccagggagc
     2821 ccacagaggc actcagtggg tggtggggtg atggatacac atctatcagg cacagggcgg
     2881 aggtgggcac cactgagttg cactcagcaa acacattggg tatcttgtgc ccaaggcctg
     2941 tatttgtgga gctgatgttc tagtgagaga cagtaaatgt gacaaaagta aaatatatca
     3001 gatggtgaga aaacagaaaa atgagatcag aagtggagat gttggggcca ggcacagtgg
     3061 cccaggcctg taatcccatc actttgggag gtgcaggcag gcagatggct tgagcccagg
     3121 aattcaagac cagtccgagc aacatagcaa aagcccttat ctgcaaaaaa ttcaaaaatt
     3181 agccaggtgt ggtggtgcgt gcccaggttc ccaggtactc ggaggctgag aggtgggagg
     3241 atgccttgag cttgagaggt tgaagctgca gtgagctgtg atcgcaccac tgcactccag
     3301 cttggttcat ggagaccctg tttttttaaa aaaagaagtg gaggtgttta caccagcaaa
     3361 atactcattt tttaagtgta attaagttga agatcaaaaa atggaaatgt ataattaaat
     3421 catacttagc aaatctaaca catgaaatgt aacatctgca tatggagaat cgtgttactt
     3481 tattgaaaaa cattaaaagt ttgagaactt aagttggatt gtggtttcgt gagcattcat
     3541 tatggtttat atatgttcca tgtatttatt attaaataga tgacaagggt
//
