LOCUS       NM_004269               1385 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens mediator complex subunit 27 (MED27), transcript
            variant 1, mRNA.
ACCESSION   NM_004269
VERSION     NM_004269.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Maroofian,R., Kaiyrzhanov,R., Cali,E., Zamani,M., Zaki,M.S.,
            Ferla,M., Tortora,D., Sadeghian,S., Saadi,S.M., Abdullah,U.,
            Karimiani,E.G., Efthymiou,S., Yesil,G., Alavi,S., Al Shamsi,A.M.,
            Tajsharghi,H., Abdel-Hamid,M.S., Saadi,N.W., Al Mutairi,F.,
            Alabdi,L., Beetz,C., Ali,Z., Toosi,M.B., Rudnik-Schoneborn,S.,
            Babaei,M., Isohanni,P., Muhammad,J., Khan,S., Al Shalan,M.,
            Hickey,S.E., Marom,D., Elhanan,E., Kurian,M.A., Marafi,D.,
            Saberi,A., Hamid,M., Spaull,R., Meng,L., Lalani,S., Maqbool,S.,
            Rahman,F., Seeger,J., Palculict,T.B., Lau,T., Murphy,D.,
            Mencacci,N.E., Steindl,K., Begemann,A., Rauch,A., Akbas,S.,
            Aslanger,A.D., Salpietro,V., Yousaf,H., Ben-Shachar,S., Ejeskar,K.,
            Al Aqeel,A.I., High,F.A., Armstrong-Javors,A.E., Zahraei,S.M.,
            Seifi,T., Zeighami,J., Shariati,G., Sedaghat,A., Asl,S.N.,
            Shahrooei,M., Zifarelli,G., Burglen,L., Ravelli,C., Zschocke,J.,
            Schatz,U.A., Ghavideldarestani,M., Kamel,W.A., Van Esch,H.,
            Hackenberg,A., Taylor,J.C., Al-Gazali,L., Bauer,P., Gleeson,J.J.,
            Alkuraya,F.S., Lupski,J.R., Galehdari,H., Azizimalamiri,R.,
            Chung,W.K., Baig,S.M., Houlden,H. and Severino,M.
  TITLE     Biallelic MED27 variants lead to variable ponto-cerebello-lental
            degeneration with movement disorders
  JOURNAL   Brain 146 (12), 5031-5043 (2023)
   PUBMED   37517035
  REMARK    GeneRIF: Biallelic MED27 variants lead to variable
            ponto-cerebello-lental degeneration with movement disorders.
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Wang,R., Yu,W., Zhu,T., Lin,F., Hua,C., Ru,L., Guo,P., Wan,X.,
            Xue,G., Guo,Z., Han,S., Lv,K., Zhang,G., Ge,H., Guo,W., Xu,L. and
            Deng,W.
  TITLE     MED27 plays a tumor-promoting role in breast cancer progression by
            targeting KLF4
  JOURNAL   Cancer Sci 114 (6), 2277-2292 (2023)
   PUBMED   36786527
  REMARK    GeneRIF: MED27 plays a tumor-promoting role in breast cancer
            progression by targeting KLF4.
REFERENCE   3  (bases 1 to 1385)
  AUTHORS   Han,X., Liu,H., Tang,X. and Zhao,Y.
  TITLE     Knockdown of mediator complex subunit 27 suppresses gastric cancer
            cell metastasis and angiogenesis via Wnt/beta-catenin pathway
  JOURNAL   Tissue Cell 79, 101973 (2022)
   PUBMED   36371844
  REMARK    GeneRIF: Knockdown of mediator complex subunit 27 suppresses
            gastric cancer cell metastasis and angiogenesis via
            Wnt/beta-catenin pathway.
REFERENCE   4  (bases 1 to 1385)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 1385)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 1385)
  AUTHORS   Sato,S., Tomomori-Sato,C., Banks,C.A., Sorokina,I., Parmely,T.J.,
            Kong,S.E., Jin,J., Cai,Y., Lane,W.S., Brower,C.S., Conaway,R.C. and
            Conaway,J.W.
  TITLE     Identification of mammalian Mediator subunits with similarities to
            yeast Mediator subunits Srb5, Srb6, Med11, and Rox3
  JOURNAL   J Biol Chem 278 (17), 15123-15127 (2003)
   PUBMED   12584197
REFERENCE   7  (bases 1 to 1385)
  AUTHORS   Mittler,G., Kremmer,E., Timmers,H.T. and Meisterernst,M.
  TITLE     Novel critical role of a human Mediator complex for basal RNA
            polymerase II transcription
  JOURNAL   EMBO Rep 2 (9), 808-813 (2001)
   PUBMED   11559591
REFERENCE   8  (bases 1 to 1385)
  AUTHORS   Malik,S., Gu,W., Wu,W., Qin,J. and Roeder,R.G.
  TITLE     The USA-derived transcriptional coactivator PC2 is a submodule of
            TRAP/SMCC and acts synergistically with other PCs
  JOURNAL   Mol Cell 5 (4), 753-760 (2000)
   PUBMED   10882111
REFERENCE   9  (bases 1 to 1385)
  AUTHORS   Ryu,S., Zhou,S., Ladurner,A.G. and Tjian,R.
  TITLE     The transcriptional cofactor complex CRSP is required for activity
            of the enhancer-binding protein Sp1
  JOURNAL   Nature 397 (6718), 446-450 (1999)
   PUBMED   9989412
REFERENCE   10 (bases 1 to 1385)
  AUTHORS   Gress,T.M., Muller-Pillasch,F., Geng,M., Zimmerhackl,F.,
            Zehetner,G., Friess,H., Buchler,M., Adler,G. and Lehrach,H.
  TITLE     A pancreatic cancer-specific expression profile
  JOURNAL   Oncogene 13 (8), 1819-1830 (1996)
   PUBMED   8895530
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP255072.1 and BC064608.1.
            
            On Nov 22, 2018 this sequence version replaced NM_004269.3.
            
            Summary: The activation of gene transcription is a multistep
            process that is triggered by factors that recognize transcriptional
            enhancer sites in DNA. These factors work with co-activators to
            direct transcriptional initiation by the RNA polymerase II
            apparatus. The protein encoded by this gene is a subunit of the
            CRSP (cofactor required for SP1 activation) complex, which, along
            with TFIID, is required for efficient activation by SP1. This
            protein is also a component of other multisubunit complexes e.g.
            thyroid hormone receptor-(TR-) associated proteins which interact
            with TR and facilitate TR function on DNA templates in conjunction
            with initiation factors and cofactors. Alternatively spliced
            transcript variants encoding multiple isoforms have been observed
            for this gene, and a pseudogene of this gene is located on the long
            arm of chromosome 5. [provided by RefSeq, Dec 2011].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC064608.1, SRR11853565.29448.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000292035.10/ ENSP00000292035.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-12                BP255072.1         21-32
            13-1385             BC064608.1         1-1373
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q34.13"
     gene            1..1385
                     /gene="MED27"
                     /gene_synonym="CRAP34; CRSP34; CRSP8; MED3; NEDSCAC;
                     TRAP37"
                     /note="mediator complex subunit 27"
                     /db_xref="GeneID:9442"
                     /db_xref="HGNC:HGNC:2377"
                     /db_xref="MIM:605044"
     exon            1..226
                     /gene="MED27"
                     /gene_synonym="CRAP34; CRSP34; CRSP8; MED3; NEDSCAC;
                     TRAP37"
                     /inference="alignment:Splign:2.1.0"
     CDS             24..959
                     /gene="MED27"
                     /gene_synonym="CRAP34; CRSP34; CRSP8; MED3; NEDSCAC;
                     TRAP37"
                     /note="isoform 1 is encoded by transcript variant 1;
                     cofactor required for Sp1 transcriptional activation,
                     subunit 8, 34kDa; p37 TRAP/SMCC/PC2 subunit; mediator of
                     RNA polymerase II transcription subunit 27; CRSP complex
                     subunit 8; transcriptional coactivator CRSP34; epididymis
                     secretory sperm binding protein"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 27 isoform 1"
                     /protein_id="NP_004260.2"
                     /db_xref="CCDS:CCDS6945.1"
                     /db_xref="GeneID:9442"
                     /db_xref="HGNC:HGNC:2377"
                     /db_xref="MIM:605044"
                     /translation="MADVINVSVNLEAFSQAISAIQALRSSVSRVFDCLKDGMRNKET
                     LEGREKAFIAHFQDNLHSVNRDLNELERLSNLVGKPSENHPLHNSGLLSLDPVQDKTP
                     LYSQLLQAYKWSNKLQYHAGLASGLLNQQSLKRSANQMGVSAKRRPKAQPTTLVLPPQ
                     YVDDVISRIDRMFPEMSIHLSRPNGTSAMLLVTLGKVLKVIVVMRSLFIDRTIVKGYN
                     ENVYTEDGKLDIWSKSNYQVFQKVTDHATTALLHYQLPQMPDVVVRSFMTWLRSYIKL
                     FQAPCQRCGKFLQDGLPPTWRDFRTLEAFHDTCRQ"
     exon            227..371
                     /gene="MED27"
                     /gene_synonym="CRAP34; CRSP34; CRSP8; MED3; NEDSCAC;
                     TRAP37"
                     /inference="alignment:Splign:2.1.0"
     exon            372..502
                     /gene="MED27"
                     /gene_synonym="CRAP34; CRSP34; CRSP8; MED3; NEDSCAC;
                     TRAP37"
                     /inference="alignment:Splign:2.1.0"
     exon            503..596
                     /gene="MED27"
                     /gene_synonym="CRAP34; CRSP34; CRSP8; MED3; NEDSCAC;
                     TRAP37"
                     /inference="alignment:Splign:2.1.0"
     exon            597..704
                     /gene="MED27"
                     /gene_synonym="CRAP34; CRSP34; CRSP8; MED3; NEDSCAC;
                     TRAP37"
                     /inference="alignment:Splign:2.1.0"
     exon            705..746
                     /gene="MED27"
                     /gene_synonym="CRAP34; CRSP34; CRSP8; MED3; NEDSCAC;
                     TRAP37"
                     /inference="alignment:Splign:2.1.0"
     exon            747..824
                     /gene="MED27"
                     /gene_synonym="CRAP34; CRSP34; CRSP8; MED3; NEDSCAC;
                     TRAP37"
                     /inference="alignment:Splign:2.1.0"
     exon            825..1385
                     /gene="MED27"
                     /gene_synonym="CRAP34; CRSP34; CRSP8; MED3; NEDSCAC;
                     TRAP37"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1366..1371
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED27"
                     /gene_synonym="CRAP34; CRSP34; CRSP8; MED3; NEDSCAC;
                     TRAP37"
                     /note="hexamer: AATAAA"
     polyA_site      1385
                     /gene="MED27"
                     /gene_synonym="CRAP34; CRSP34; CRSP8; MED3; NEDSCAC;
                     TRAP37"
                     /note="major polyA site"
ORIGIN      
        1 agagctgctg tggcggcggc aacatggcgg acgtgataaa tgtcagtgtg aacctggagg
       61 ccttttccca ggccattagt gccatccagg cgctgcgctc cagcgtgagc agggtgttcg
      121 actgcctgaa ggatgggatg cggaacaagg agacgctgga gggccgggag aaggccttta
      181 ttgcgcactt ccaggacaac ttacattcgg tcaaccggga cctcaatgag ctggaacgtc
      241 tgagcaatct ggtaggcaag ccatctgaga accatcctct tcataacagt gggctgttaa
      301 gcctggatcc tgtgcaggac aaaactcctc tctatagtca actccttcaa gcatataagt
      361 ggtcaaacaa gttgcagtac catgcaggac tagcatctgg ccttttaaat cagcagtcat
      421 tgaagcgttc cgctaatcag atgggagtat ctgccaaacg tagaccaaag gctcagccca
      481 caactcttgt cctaccacct caatatgttg atgatgtgat cagccgcatt gacaggatgt
      541 ttcctgaaat gtccatccac ttatccagac ccaatggaac atcagcaatg cttctggtga
      601 ccttgggaaa ggtgttgaaa gtgatcgtcg tcatgcggag cctgttcatt gatcgaacaa
      661 tagtaaaggg atataacgag aatgtctaca cagaagatgg caagcttgat atatggtcca
      721 aatccaacta tcaagtattc cagaaggtga cagaccatgc caccactgcc ctgctccact
      781 atcagctgcc ccagatgccg gatgtcgtgg tccgatcctt catgacctgg ttaagaagtt
      841 acataaagct gttccaggcc ccgtgccagc gctgcgggaa gtttctgcag gacggccttc
      901 ccccgacatg gagggatttc cgaaccctcg aagccttcca tgacacctgc cggcagtagc
      961 ccccacgctg gccccagcct cagaccccac ccagcacctt cccagacacg caggaagccc
     1021 acagaaggct cagctggttc ctcactgccc agatgtgtac agctgctcct ccctttcata
     1081 aagcagcgcc atgtgtgcag aggccactct tgaagagcag actccctctg tggctgatgg
     1141 gactaattat tcccactagc cagcggactg aaggcaaaga agacctttct agaacctggt
     1201 agaaggaagc tgtgcagcat gctcctcgtc catgtgtgtc ggcagtgctg gtgtctgtcg
     1261 tctccgcgag ctgttactgg aatgagccct tgtgttcatg ggtatcgtca tgcggggttc
     1321 ttgtgttttg tggggcttgg gttttggtaa cttattttta taagcaataa accttttgta
     1381 tcctg
//
