LOCUS       NM_004831               3172 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens mediator complex subunit 26 (MED26), mRNA.
ACCESSION   NM_004831
VERSION     NM_004831.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 3172)
  AUTHORS   Lens,Z., Cantrelle,F.X., Peruzzini,R., Hanoulle,X., Dewitte,F.,
            Ferreira,E., Baert,J.L., Monte,D., Aumercier,M., Villeret,V.,
            Verger,A. and Landrieu,I.
  TITLE     Solution Structure of the N-Terminal Domain of Mediator Subunit
            MED26 and Molecular Characterization of Its Interaction with EAF1
            and TAF7
  JOURNAL   J Mol Biol 429 (20), 3043-3055 (2017)
   PUBMED   28893534
  REMARK    GeneRIF: EAF1 (239-268) and TAF7 (205-235) peptide interactions
            were both mapped to the same groove formed by H3 and H4 helices of
            MED26 N-terminal domain.
REFERENCE   4  (bases 1 to 3172)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   5  (bases 1 to 3172)
  AUTHORS   Takahashi,H., Takigawa,I., Watanabe,M., Anwar,D., Shibata,M.,
            Tomomori-Sato,C., Sato,S., Ranjan,A., Seidel,C.W., Tsukiyama,T.,
            Mizushima,W., Hayashi,M., Ohkawa,Y., Conaway,J.W., Conaway,R.C. and
            Hatakeyama,S.
  TITLE     MED26 regulates the transcription of snRNA genes through the
            recruitment of little elongation complex
  JOURNAL   Nat Commun 6, 5941 (2015)
   PUBMED   25575120
  REMARK    GeneRIF: the MED26-NTD functions as a molecular switch in the
            exchange of TBP-associated factor 7 (TAF7) for LEC to facilitate
            the transition from initiation to elongation during transcription
            of a subset of snRNA genes
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3172)
  AUTHORS   Naar,A.M., Taatjes,D.J., Zhai,W., Nogales,E. and Tjian,R.
  TITLE     Human CRSP interacts with RNA polymerase II CTD and adopts a
            specific CTD-bound conformation
  JOURNAL   Genes Dev 16 (11), 1339-1344 (2002)
   PUBMED   12050112
  REMARK    GeneRIF: Human CRSP interacts with RNA polymerase II CTD and adopts
            a specific CTD-bound conformation
REFERENCE   7  (bases 1 to 3172)
  AUTHORS   Mittler,G., Kremmer,E., Timmers,H.T. and Meisterernst,M.
  TITLE     Novel critical role of a human Mediator complex for basal RNA
            polymerase II transcription
  JOURNAL   EMBO Rep 2 (9), 808-813 (2001)
   PUBMED   11559591
REFERENCE   8  (bases 1 to 3172)
  AUTHORS   Booth,V., Koth,C.M., Edwards,A.M. and Arrowsmith,C.H.
  TITLE     Structure of a conserved domain common to the transcription factors
            TFIIS, elongin A, and CRSP70
  JOURNAL   J Biol Chem 275 (40), 31266-31268 (2000)
   PUBMED   10811649
REFERENCE   9  (bases 1 to 3172)
  AUTHORS   Naar,A.M., Beaurang,P.A., Zhou,S., Abraham,S., Solomon,W. and
            Tjian,R.
  TITLE     Composite co-activator ARC mediates chromatin-directed
            transcriptional activation
  JOURNAL   Nature 398 (6730), 828-832 (1999)
   PUBMED   10235267
REFERENCE   10 (bases 1 to 3172)
  AUTHORS   Ryu,S., Zhou,S., Ladurner,A.G. and Tjian,R.
  TITLE     The transcriptional cofactor complex CRSP is required for activity
            of the enhancer-binding protein Sp1
  JOURNAL   Nature 397 (6718), 446-450 (1999)
   PUBMED   9989412
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC024075.4, AF104253.1, AK128435.1 and AC008764.9.
            
            On Nov 30, 2019 this sequence version replaced NM_004831.4.
            
            Summary: The activation of gene transcription is a multistep
            process that is triggered by factors that recognize transcriptional
            enhancer sites in DNA. These factors work with co-activators to
            direct transcriptional initiation by the RNA polymerase II
            apparatus. The protein encoded by this gene is a subunit of the
            CRSP (cofactor required for SP1 activation) complex, which, along
            with TFIID, is required for efficient activation by SP1. This
            protein is also a component of other multisubunit complexes e.g.
            thyroid hormone receptor-(TR-) associated proteins which interact
            with TR and facilitate TR function on DNA templates in conjunction
            with initiation factors and cofactors. [provided by RefSeq, Jul
            2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC110430.1, SRR14038193.3020669.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000263390.8/ ENSP00000263390.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-261               AC024075.4         20290-20550         c
            262-726             AF104253.1         1-465
            727-3169            AK128435.1         1414-3856
            3170-3172           AC008764.9         122161-122163       c
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.11"
     gene            1..3172
                     /gene="MED26"
                     /gene_synonym="CRSP7; CRSP70"
                     /note="mediator complex subunit 26"
                     /db_xref="GeneID:9441"
                     /db_xref="HGNC:HGNC:2376"
                     /db_xref="MIM:605043"
     exon            1..333
                     /gene="MED26"
                     /gene_synonym="CRSP7; CRSP70"
                     /inference="alignment:Splign:2.1.0"
     CDS             262..2064
                     /gene="MED26"
                     /gene_synonym="CRSP7; CRSP70"
                     /note="cofactor required for Sp1 transcriptional
                     activation, subunit 7 (70kD); cofactor required for Sp1
                     transcriptional activation, subunit 7, 70kDa; ARC70; CRSP
                     complex subunit 7; transcriptional coactivator CRSP70;
                     activator-recruited cofactor 70 kDa component; cofactor
                     required for Sp1 transcriptional activation subunit 7"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 26"
                     /protein_id="NP_004822.2"
                     /db_xref="CCDS:CCDS12347.1"
                     /db_xref="GeneID:9441"
                     /db_xref="HGNC:HGNC:2376"
                     /db_xref="MIM:605043"
                     /translation="MTAAPASPQQIRDRLLQAIDPQSNIRNMVAVLEVISSLEKYPIT
                     KEALEETRLGKLINDVRKKTKNEELAKRAKKLLRSWQKLIEPAHQHEAALRGLAGATG
                     SANGGAHNCRPEVGAAGPPRSIHDLKSRNDLQRLPGQRLDRLGSRKRRGDQRDLGHPG
                     PPPKVSKASHDPLVPNSSPLPTNGISGSPESFASSLDGSGHAGPEGSRLERDENDKHS
                     GKIPVNAVRPHTSSPGLGKPPGPCLQPKASVLQQLDRVDETPGPPHPKGPPRCSFSPR
                     NSRHEGSFARQQSLYAPKGSVPSPSPRPQALDATQVPSPLPLAQPSTPPVRRLELLPS
                     AESPVCWLEQPESHQRLAGPGCKAGLSPAEPLLSRAGFSPDSSKADSDAASSGGSDSK
                     KKKRYRPRDYTVNLDGQVAEAGVKPVRLKERKLTFDPMTRQIKPLTQKEPVRADSPVH
                     MEQQSRTELDKQEAKASLQSPFEQTNWKELSRNEIIQSYLSRQSSLLSSSGAQTPGAH
                     HFMSEYLKQEESTRQGARQLHVLVPQSPPTDLPGLTREVTQDDLDRIQASQWPGVNGC
                     QDTQGNWYDWTQCISLDPHGDDGRLNILPYVCLD"
     exon            334..408
                     /gene="MED26"
                     /gene_synonym="CRSP7; CRSP70"
                     /inference="alignment:Splign:2.1.0"
     exon            409..3172
                     /gene="MED26"
                     /gene_synonym="CRSP7; CRSP70"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2731..2736
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED26"
                     /gene_synonym="CRSP7; CRSP70"
                     /note="hexamer: AAGAAA"
     polyA_site      2747
                     /gene="MED26"
                     /gene_synonym="CRSP7; CRSP70"
                     /note="major polyA site"
     regulatory      3018..3023
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED26"
                     /gene_synonym="CRSP7; CRSP70"
                     /note="hexamer: CATAAA"
     polyA_site      3041
                     /gene="MED26"
                     /gene_synonym="CRSP7; CRSP70"
                     /note="major polyA site"
ORIGIN      
        1 actggagttt ggttctcgag gcggcggcag cgagcggcgg cggctccggc ggctcctcct
       61 cctcctttgg tggcggcggc ggcccgggac cgagacccgg ccccggctcc cctctccgcc
      121 accccgcgcg cccccaaccc cccggcgccg cggggaacat gcggctccgg ctcccggagg
      181 ccggcgagtg agtgaccccc gtcccccgcc tcagcccgcc cgcccgctgg cccggccgca
      241 accccccgcc tcgcccaggc aatgacagcg gctccggcgt ctccgcagca gatcagggac
      301 cggctgctgc aggccatcga cccccagagc aacatccgga acatggtggc ggtgctggaa
      361 gtcatctcca gcctggagaa ataccctatt accaaagagg cacttgagga aacacgactt
      421 gggaagctca tcaacgacgt ccgcaagaaa accaagaacg aggagctcgc caagcgggcc
      481 aagaagctgc tgcggagctg gcagaagctc atcgagccgg cacaccagca tgaggcggcg
      541 ctgcgggggc tggcgggggc caccggctct gccaacgggg gcgcacacaa ctgccggccg
      601 gaggtggggg cggctggccc acccaggagc atccatgacc tgaagagccg caatgacctc
      661 cagaggctgc ccgggcagcg gctggacagg ctgggcagcc gcaagcgccg gggtgaccag
      721 cgtgacctcg gccacccagg gccgccaccc aaggtctcca aagctagcca cgaccccctg
      781 gtccccaact catcccccct ccccaccaac gggatcagtg ggagtccaga gagcttcgcc
      841 agctccctgg atggcagtgg gcatgcaggc ccagagggca gccgcctgga gcgtgacgag
      901 aatgacaagc acagtggcaa gatccccgtc aacgccgtgc gaccgcacac cagctccccg
      961 ggcctgggca agccccctgg accctgcttg cagccaaagg cttcggtgct gcagcagctg
     1021 gacagggtgg acgagactcc ggggcctccc catcccaagg gaccccctcg ctgctctttc
     1081 agtcctcgga actcacggca tgagggctcc tttgcccggc agcagagctt gtatgcaccc
     1141 aagggctccg tgcccagccc ctcaccgcgg ccccaggcac tcgatgccac acaggtgccg
     1201 tcaccgcttc cactggcaca gccgtccaca ccccccgtac ggcggctcga gctgctgccc
     1261 agtgcggaaa gcccagtgtg ctggcttgag cagcctgaga gccaccagcg gctggcgggg
     1321 ccgggctgca aggcagggct gtccccagcc gagcccctcc tgtcccgggc aggcttttcc
     1381 ccagactcct ccaaggcgga cagtgatgct gcctcctcag ggggctcgga cagtaaaaag
     1441 aagaagaggt accgacctcg agactatacg gttaacttgg acgggcaggt ggctgaggcg
     1501 ggcgtcaagc ctgtccggtt aaaagagcgg aagctcacct ttgaccccat gacgagacag
     1561 atcaaacctc tgacccagaa agagccagtg cgggcagaca gccctgtgca catggagcag
     1621 cagtccagga cagagctgga caagcaggag gccaaggcca gcctccagag ccccttcgaa
     1681 cagacgaact ggaaggagct gtcacgcaac gagatcatcc agtcctacct gagccggcag
     1741 agcagcctgc tctcatcatc gggcgcgcag accccagggg ctcaccactt catgtctgag
     1801 tacctgaagc aggaggagag cacccggcaa ggggccaggc agctgcatgt gctggtgcct
     1861 caaagcccgc ccacggacct ccctggtctg acccgggagg tcacacagga cgatctcgac
     1921 agaatccagg ccagccagtg gccgggggtg aacgggtgtc aggacacaca gggtaactgg
     1981 tatgactgga cgcagtgcat atcgctcgat ccgcacggcg acgacgggcg cttgaacatt
     2041 ctgccttatg tctgcttgga ctgaccggcc tgtcagccac aaagtgcatt cccatcttgc
     2101 agaagccggg tgggcgggca ggcaggtggg caggtggccg gggcccagct gcctcctgcg
     2161 ctgggaactc ggggagtccg gcccaggcgg gagggagggg gcgggagtca cgcggtctct
     2221 ctgcgctctt ccctcaaaac tctttttgtg aaatgctgct accagtttac taacaaaatt
     2281 gcgaagaagg accgcgtgga aggaaggacg gcaaagtgag ttcagaactc tatagcaaac
     2341 cagctataaa aagcgttagt ccctcacccc caaagaggtg cggacacttt ccagcacctg
     2401 aaagctgggg cctcagttgc aggcaggcac agaaaacctg tgggagaggt cacctttaac
     2461 acagcaacag aagcacgcat ctcatctctc ttgcattttc tgttctttaa tttggccctg
     2521 agtgtcttga gacagtgggc catgccactg cctctgtttg ctccctaccc agccactgtc
     2581 aggccagtgt tgtctgtcat gtgccagaag cttctcagct gtggtgagcc actggcattg
     2641 ggcaggcggg ccagctggcc agccagtgtg acccacactc catgggcagc cacctgttta
     2701 tatctggttt cagttataac ccagttttta aagaaatgct gcaaaaattt aagttttctc
     2761 atgtttattt taaacaattc ctcttccatc agaccaccag acaaaaaaac ctttcctttc
     2821 ttcctccctt ttcttttttc ccttcctgta cacatctaga aaacttcacc tttctagagt
     2881 ccccccaaaa caacacaaaa gttattgtgg gtgctactgg ttttatgctg tactgtgggg
     2941 cgagggggaa gacctgtctg tacgctggaa acactcataa ccattccttt agattcgttt
     3001 gccttatggt tatttgtcat aaacgcgatt tgcaaaaaca aggagcctta gtgaatgtac
     3061 agtacctaga cttctgtgtt ctcaggacgc agaagggagc aactttgcta tttggtccag
     3121 taagtggaca ccttgtgata taaatgtgga aataaaaaaa aaaaaaaaac ca
//
