LOCUS       NM_030973               2332 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens mediator complex subunit 25 (MED25), transcript
            variant 1, mRNA.
ACCESSION   NM_030973
VERSION     NM_030973.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Van Royen,T., Sedeyn,K., Moschonas,G.D., Toussaint,W.,
            Vuylsteke,M., Van Haver,D., Impens,F., Eyckerman,S., Lemmens,I.,
            Tavernier,J., Schepens,B. and Saelens,X.
  TITLE     An Unexpected Encounter: Respiratory Syncytial Virus Nonstructural
            Protein 1 Interacts with Mediator Subunit MED25
  JOURNAL   J Virol 96 (19), e0129722 (2022)
   PUBMED   36102648
  REMARK    GeneRIF: An Unexpected Encounter: Respiratory Syncytial Virus
            Nonstructural Protein 1 Interacts with Mediator Subunit MED25.
            Review article
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 2332)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 2332)
  AUTHORS   Jeffery,H.M. and Weinzierl,R.O.J.
  TITLE     Multivalent and Bidirectional Binding of Transcriptional
            Transactivation Domains to the MED25 Coactivator
  JOURNAL   Biomolecules 10 (9), 1205 (2020)
   PUBMED   32825095
  REMARK    GeneRIF: Multivalent and Bidirectional Binding of Transcriptional
            Transactivation Domains to the MED25 Coactivator.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2332)
  AUTHORS   Wang,C., McCarty,I.M., Balazs,L., Li,Y. and Steiner,M.S.
  TITLE     A prostate-derived cDNA that is mapped to human chromosome 19
            encodes a novel protein
  JOURNAL   Biochem Biophys Res Commun 296 (2), 281-287 (2002)
   PUBMED   12163014
  REMARK    GeneRIF: cloned a prostate-derived cDNA encoding a novel protein
            with a predicted molecular weight of 78 kDa (P(78)), and precisely
            mapped the cDNA sequence to chromosome 19
REFERENCE   6  (bases 1 to 2332)
  AUTHORS   Mittler,G., Kremmer,E., Timmers,H.T. and Meisterernst,M.
  TITLE     Novel critical role of a human Mediator complex for basal RNA
            polymerase II transcription
  JOURNAL   EMBO Rep 2 (9), 808-813 (2001)
   PUBMED   11559591
REFERENCE   7  (bases 1 to 2332)
  AUTHORS   Leal,A., Morera,B., Del Valle,G., Heuss,D., Kayser,C., Berghoff,M.,
            Villegas,R., Hernandez,E., Mendez,M., Hennies,H.C., Neundorfer,B.,
            Barrantes,R., Reis,A. and Rautenstrauss,B.
  TITLE     A second locus for an axonal form of autosomal recessive
            Charcot-Marie-Tooth disease maps to chromosome 19q13.3
  JOURNAL   Am J Hum Genet 68 (1), 269-274 (2001)
   PUBMED   11112660
REFERENCE   8  (bases 1 to 2332)
  AUTHORS   Naar,A.M., Beaurang,P.A., Zhou,S., Abraham,S., Solomon,W. and
            Tjian,R.
  TITLE     Composite co-activator ARC mediates chromatin-directed
            transcriptional activation
  JOURNAL   Nature 398 (6730), 828-832 (1999)
   PUBMED   10235267
REFERENCE   9  (bases 1 to 2332)
  AUTHORS   Bird,T.D.
  TITLE     Charcot-Marie-Tooth Hereditary Neuropathy Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301532
REFERENCE   10 (bases 1 to 2332)
  AUTHORS   Bird,T.D.
  TITLE     Charcot-Marie-Tooth Neuropathy Type 2 - RETIRED CHAPTER, FOR
            HISTORICAL REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301462
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC410997.1 and BC065297.1.
            
            On Feb 10, 2020 this sequence version replaced NM_030973.3.
            
            Summary: This gene encodes a component of the transcriptional
            coactivator complex termed the Mediator complex. This complex is
            required for transcription of most RNA polymerase II-dependent
            genes. The encoded protein plays a role in chromatin modification
            and in preinitiation complex assembly. Mutations in this gene are
            associated with Charcot-Marie-Tooth disease type 2B2. [provided by
            RefSeq, Apr 2010].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC065297.1, AK289460.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000312865.10/ ENSP00000326767.5
            RefSeq Select criteria :: based on manual assertion, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-17                DC410997.1         11-27
            18-2332             BC065297.1         1-2315
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.33"
     gene            1..2332
                     /gene="MED25"
                     /gene_synonym="ACID1; ARC92; BVSYS; CMT2B2; P78; PTOV2;
                     TCBAP0758"
                     /note="mediator complex subunit 25"
                     /db_xref="GeneID:81857"
                     /db_xref="HGNC:HGNC:28845"
                     /db_xref="MIM:610197"
     exon            1..187
                     /gene="MED25"
                     /gene_synonym="ACID1; ARC92; BVSYS; CMT2B2; P78; PTOV2;
                     TCBAP0758"
                     /inference="alignment:Splign:2.1.0"
     CDS             54..2297
                     /gene="MED25"
                     /gene_synonym="ACID1; ARC92; BVSYS; CMT2B2; P78; PTOV2;
                     TCBAP0758"
                     /note="isoform 1 is encoded by transcript variant 1;
                     prostate-derived protein 78 kDa; mediator of RNA
                     polymerase II transcription, subunit 25 homolog;
                     ARC/mediator transcriptional coactivator subunit; mediator
                     of RNA polymerase II transcription subunit 25;
                     activator-recruited cofactor 92 kDa component; activator
                     interaction domain-containing protein 1"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 25 isoform 1"
                     /protein_id="NP_112235.2"
                     /db_xref="CCDS:CCDS33075.1"
                     /db_xref="GeneID:81857"
                     /db_xref="HGNC:HGNC:28845"
                     /db_xref="MIM:610197"
                     /translation="MVPGSEGPARAGSVVADVVFVIEGTANLGPYFEGLRKHYLLPAI
                     EYFNGGPPAETDFGGDYGGTQYSLVVFNTVDCAPESYVQCHAPTSSAYEFVTWLDGIK
                     FMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTT
                     YSGCTTENLVQQIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQPPTDVSQDPR
                     HMVLVRGLVLPVGGGSAPGPLQSKQPVPLPPAAPSGATLSAAPQQPLPPVPPQYQVPG
                     NLSAAQVAAQNAVEAAKNQKAGLGPRFSPITPLQQAAPGVGPPFSQAPAPQLPPGPPG
                     APKPPPASQPSLVSTVAPGSGLAPTAQPGAPSMAGTVAPGGVSGPSPAQLGAPALGGQ
                     QSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIM
                     QLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEV
                     RVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQQRGMGGQQAPP
                     GLGPILEDQARPSQNLLQLRPPQPQPQGTVGASGATGQPQPQGTAQPPPGAPQGPPGA
                     ASGPPPPGPILRPQNPGANPQLRSLLLNPPPPQTGVPPPQASLHHLQPPGAPALLPPP
                     HQGLGQPQLGPPLLHPPPAQSWPAQLPPRAPLPGQMLLSGGPRGPVPQPGLQPSVMED
                     DILMDLI"
     exon            188..233
                     /gene="MED25"
                     /gene_synonym="ACID1; ARC92; BVSYS; CMT2B2; P78; PTOV2;
                     TCBAP0758"
                     /inference="alignment:Splign:2.1.0"
     exon            234..358
                     /gene="MED25"
                     /gene_synonym="ACID1; ARC92; BVSYS; CMT2B2; P78; PTOV2;
                     TCBAP0758"
                     /inference="alignment:Splign:2.1.0"
     exon            359..457
                     /gene="MED25"
                     /gene_synonym="ACID1; ARC92; BVSYS; CMT2B2; P78; PTOV2;
                     TCBAP0758"
                     /inference="alignment:Splign:2.1.0"
     exon            458..578
                     /gene="MED25"
                     /gene_synonym="ACID1; ARC92; BVSYS; CMT2B2; P78; PTOV2;
                     TCBAP0758"
                     /inference="alignment:Splign:2.1.0"
     exon            579..741
                     /gene="MED25"
                     /gene_synonym="ACID1; ARC92; BVSYS; CMT2B2; P78; PTOV2;
                     TCBAP0758"
                     /inference="alignment:Splign:2.1.0"
     exon            742..872
                     /gene="MED25"
                     /gene_synonym="ACID1; ARC92; BVSYS; CMT2B2; P78; PTOV2;
                     TCBAP0758"
                     /inference="alignment:Splign:2.1.0"
     exon            873..960
                     /gene="MED25"
                     /gene_synonym="ACID1; ARC92; BVSYS; CMT2B2; P78; PTOV2;
                     TCBAP0758"
                     /inference="alignment:Splign:2.1.0"
     exon            961..1154
                     /gene="MED25"
                     /gene_synonym="ACID1; ARC92; BVSYS; CMT2B2; P78; PTOV2;
                     TCBAP0758"
                     /inference="alignment:Splign:2.1.0"
     exon            1155..1283
                     /gene="MED25"
                     /gene_synonym="ACID1; ARC92; BVSYS; CMT2B2; P78; PTOV2;
                     TCBAP0758"
                     /inference="alignment:Splign:2.1.0"
     exon            1284..1369
                     /gene="MED25"
                     /gene_synonym="ACID1; ARC92; BVSYS; CMT2B2; P78; PTOV2;
                     TCBAP0758"
                     /inference="alignment:Splign:2.1.0"
     exon            1370..1427
                     /gene="MED25"
                     /gene_synonym="ACID1; ARC92; BVSYS; CMT2B2; P78; PTOV2;
                     TCBAP0758"
                     /inference="alignment:Splign:2.1.0"
     exon            1428..1535
                     /gene="MED25"
                     /gene_synonym="ACID1; ARC92; BVSYS; CMT2B2; P78; PTOV2;
                     TCBAP0758"
                     /inference="alignment:Splign:2.1.0"
     exon            1536..1727
                     /gene="MED25"
                     /gene_synonym="ACID1; ARC92; BVSYS; CMT2B2; P78; PTOV2;
                     TCBAP0758"
                     /inference="alignment:Splign:2.1.0"
     exon            1728..1799
                     /gene="MED25"
                     /gene_synonym="ACID1; ARC92; BVSYS; CMT2B2; P78; PTOV2;
                     TCBAP0758"
                     /inference="alignment:Splign:2.1.0"
     exon            1800..2018
                     /gene="MED25"
                     /gene_synonym="ACID1; ARC92; BVSYS; CMT2B2; P78; PTOV2;
                     TCBAP0758"
                     /inference="alignment:Splign:2.1.0"
     exon            2019..2199
                     /gene="MED25"
                     /gene_synonym="ACID1; ARC92; BVSYS; CMT2B2; P78; PTOV2;
                     TCBAP0758"
                     /inference="alignment:Splign:2.1.0"
     exon            2200..2332
                     /gene="MED25"
                     /gene_synonym="ACID1; ARC92; BVSYS; CMT2B2; P78; PTOV2;
                     TCBAP0758"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2311..2316
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED25"
                     /gene_synonym="ACID1; ARC92; BVSYS; CMT2B2; P78; PTOV2;
                     TCBAP0758"
                     /note="hexamer: AATAAA"
     polyA_site      2332
                     /gene="MED25"
                     /gene_synonym="ACID1; ARC92; BVSYS; CMT2B2; P78; PTOV2;
                     TCBAP0758"
                     /note="major polyA site"
ORIGIN      
        1 attccgcggc gtcggctgcg gctgcagtgg tggtggcggg taccgcacgg ggtatggtcc
       61 ccgggtccga gggcccggcc cgcgccggga gcgtggtggc cgacgtggtg tttgtgattg
      121 agggtacggc caacctggga ccctacttcg aggggctccg caagcactac ctgctcccgg
      181 ccatcgagta ttttaatggt ggtcctcctg ctgagacgga cttcggggga gactatgggg
      241 ggacccagta cagcctcgtg gtgttcaaca cagtggactg cgctcccgag tcctacgtac
      301 aatgtcacgc tcccaccagc agcgcctatg agtttgtcac ctggctcgat ggcattaagt
      361 tcatgggcgg gggtggtgag agctgcagcc tcatcgcgga aggactcagc acagccttgc
      421 agctgtttga tgacttcaag aagatgcgcg agcagattgg ccagacgcac cgggtctgcc
      481 tcctcatctg caactcaccc ccatacttgt tgcctgctgt tgagagcacc acgtactctg
      541 gatgcacaac tgagaatctt gtgcagcaga ttggggagcg ggggatccac ttctccattg
      601 tgtctccccg gaagctgcct gcgcttcggc ttctgtttga gaaggcagcc cccccggcct
      661 tgctggagcc gctgcagcct ccgacagatg tgagccagga cccgaggcac atggtgctgg
      721 ttcggggact cgtgctgcct gttgggggtg gctcagcccc aggccccctc cagtcaaagc
      781 agccagtccc cctgcctccc gccgcaccct caggtgccac tctctcagca gccccccagc
      841 agcctctgcc ccccgtcccc ccgcagtacc aggttcccgg gaacctgagt gcagctcagg
      901 tggccgcgca gaatgcagtg gaggctgcca agaaccagaa ggctgggctg ggccctcgct
      961 tctcgcccat cacccctctc caacaagctg ctcccggagt gggtcccccc ttcagccagg
     1021 ccccagctcc ccaactaccc ccaggacccc ctggcgcccc caagccacca cctgcttccc
     1081 agcccagtct ggtctccact gtggcccctg gctccggcct ggctcccacg gcacagcccg
     1141 gggcaccgtc catggcaggc actgtggccc caggaggggt gagcggccct tccccagccc
     1201 agctgggagc cccagccctc ggtgggcagc agtcagtctc caataagctt ctggcctgga
     1261 gcggggtcct ggagtggcaa gagaaaccca aacctgcctc agtggatgcc aacaccaagc
     1321 tgacgcggtc actgccctgc caggtctacg tgaatcatgg cgagaacctg aagacggagc
     1381 agtggcccca gaagctgatc atgcagctca tcccccagca gctgctgacc accctgggcc
     1441 ctttgttccg gaactcaagg atggtccagt tccatttcac caacaaggac ctggagtctc
     1501 tcaaaggcct ctaccgcatc atgggcaacg gcttcgcggg ctgcgtgcac ttcccccaca
     1561 cggcgccctg tgaggtgcgc gtgctcatgc tcctgtactc gtccaagaag aagatcttca
     1621 tgggcctcat cccctacgac cagagcggct tcgtcaacgg catccggcag gtcatcacca
     1681 accacaagca ggtccagcag cagaagctgg agcagcagca gcgaggaatg gggggacagc
     1741 aggcaccccc agggctgggg cccattctgg aggaccaagc caggccctca cagaatctgc
     1801 tccagctccg cccaccgcag ccccagcctc agggtaccgt aggggcctct ggggccacgg
     1861 ggcagcccca gccccaaggt actgcccagc ccccgccagg tgcccctcaa ggccctcctg
     1921 gagcagcttc tggcccaccc cctcctggac ccatccttcg gccccagaac cctggggcca
     1981 accctcagct gcgaagcctc ctcctcaacc caccaccgcc gcagactggg gtgcccccac
     2041 cccaggcctc cctccaccac ctccagccac caggggctcc tgcgctgctg cctccgccgc
     2101 accagggcct ggggcagccc cagttggggc ccccactcct gcatccacca cctgcccagt
     2161 cctggcccgc acaacttccc cctcgggctc cactgccagg tcagatgctg ctgagcgggg
     2221 gtccccgggg cccggtcccc cagccgggcc tgcagcccag cgtcatggag gacgacatcc
     2281 tcatggatct catctgaatc cccaacaccc aataaagttc ctttttaaca ca
//
