LOCUS       NM_004830               5247 bp    mRNA    linear   PRI 29-OCT-2024
DEFINITION  Homo sapiens mediator complex subunit 23 (MED23), transcript
            variant 1, mRNA.
ACCESSION   NM_004830
VERSION     NM_004830.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Li,Y., Sun,Y., Jia,B., Ma,Z. and Zhou,R.
  TITLE     MED23 depletion induces premature senescence in NSCLC cells by
            interacting with BCLAF1 and then suppressing NUPR1 expression
  JOURNAL   Biochem Biophys Res Commun 734, 150754 (2024)
   PUBMED   39366174
  REMARK    GeneRIF: MED23 depletion induces premature senescence in NSCLC
            cells by interacting with BCLAF1 and then suppressing NUPR1
            expression.
REFERENCE   2  (bases 1 to 5247)
  AUTHORS   Bamaga,A., Muthaffar,O., Alyazidi,A., Bahowarth,S., Shawli,M.,
            Alotibi,F., Alsehemi,M., Almohammal,M., Alawwadh,A. and Alghamdi,N.
  TITLE     MED23 pathogenic variant: genomic-phenotypic analysis
  JOURNAL   J Med Life 17 (5), 500-507 (2024)
   PUBMED   39144687
  REMARK    GeneRIF: MED23 pathogenic variant: genomic-phenotypic analysis.
REFERENCE   3  (bases 1 to 5247)
  AUTHORS   Zhang,N., Liu,X., Huang,L., Zeng,J., Ma,C., Han,L., Li,W., Yu,J.
            and Yang,M.
  TITLE     LINC00921 reduces lung cancer radiosensitivity by destabilizing
            NUDT21 and driving aberrant MED23 alternative polyadenylation
  JOURNAL   Cell Rep 42 (12), 113479 (2023)
   PUBMED   37999979
  REMARK    GeneRIF: LINC00921 reduces lung cancer radiosensitivity by
            destabilizing NUDT21 and driving aberrant MED23 alternative
            polyadenylation.
REFERENCE   4  (bases 1 to 5247)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 5247)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 5247)
  AUTHORS   Ko,L., Cardona,G.R. and Chin,W.W.
  TITLE     Thyroid hormone receptor-binding protein, an LXXLL motif-containing
            protein, functions as a general coactivator
  JOURNAL   Proc Natl Acad Sci U S A 97 (11), 6212-6217 (2000)
   PUBMED   10823961
REFERENCE   7  (bases 1 to 5247)
  AUTHORS   Boyer,T.G., Martin,M.E., Lees,E., Ricciardi,R.P. and Berk,A.J.
  TITLE     Mammalian Srb/Mediator complex is targeted by adenovirus E1A
            protein
  JOURNAL   Nature 399 (6733), 276-279 (1999)
   PUBMED   10353252
REFERENCE   8  (bases 1 to 5247)
  AUTHORS   Naar,A.M., Beaurang,P.A., Zhou,S., Abraham,S., Solomon,W. and
            Tjian,R.
  TITLE     Composite co-activator ARC mediates chromatin-directed
            transcriptional activation
  JOURNAL   Nature 398 (6730), 828-832 (1999)
   PUBMED   10235267
REFERENCE   9  (bases 1 to 5247)
  AUTHORS   Rachez,C., Lemon,B.D., Suldan,Z., Bromleigh,V., Gamble,M.,
            Naar,A.M., Erdjument-Bromage,H., Tempst,P. and Freedman,L.P.
  TITLE     Ligand-dependent transcription activation by nuclear receptors
            requires the DRIP complex
  JOURNAL   Nature 398 (6730), 824-828 (1999)
   PUBMED   10235266
REFERENCE   10 (bases 1 to 5247)
  AUTHORS   Ryu,S., Zhou,S., Ladurner,A.G. and Tjian,R.
  TITLE     The transcriptional cofactor complex CRSP is required for activity
            of the enhancer-binding protein Sp1
  JOURNAL   Nature 397 (6718), 446-450 (1999)
   PUBMED   9989412
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL121575.24, DB453121.1,
            AB033042.1 and AI249380.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_004830.3.
            
            Summary: The activation of gene transcription is a multistep
            process that is triggered by factors that recognize transcriptional
            enhancer sites in DNA. These factors work with co-activators to
            direct transcriptional initiation by the RNA polymerase II
            apparatus. The protein encoded by this gene is a subunit of the
            CRSP (cofactor required for SP1 activation) complex, which, along
            with TFIID, is required for efficient activation by SP1. This
            protein is also a component of other multisubunit complexes e.g.
            thyroid hormone receptor-(TR-) associated proteins which interact
            with TR and facilitate TR function on DNA templates in conjunction
            with initiation factors and cofactors. This protein also acts as a
            metastasis suppressor. Several alternatively spliced transcript
            variants encoding different isoforms have been described for this
            gene. [provided by RefSeq, Jul 2012].
            
            Transcript Variant: This variant (1) encodes isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB033042.1, AF105332.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000368068.8/ ENSP00000357047.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 AL121575.24        128545-128547       c
            4-34                DB453121.1         1-31
            35-5230             AB033042.1         1-5196
            5231-5241           AI249380.1         3-13                c
            5242-5247           AL121575.24        87037-87042         c
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q23.2"
     gene            1..5247
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /note="mediator complex subunit 23"
                     /db_xref="GeneID:9439"
                     /db_xref="HGNC:HGNC:2372"
                     /db_xref="MIM:605042"
     exon            1..232
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /inference="alignment:Splign:2.1.0"
     CDS             194..4300
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /note="isoform a is encoded by transcript variant 1; 133
                     kDa transcriptional co-activator; 130 kDa transcriptional
                     co-activator; vitamin D3 receptor interacting protein;
                     mediator of RNA polymerase II transcription subunit 23;
                     activator-recruited cofactor 130 kDa component; cofactor
                     required for Sp1 transcriptional activation subunit 3"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 23 isoform a"
                     /protein_id="NP_004821.2"
                     /db_xref="CCDS:CCDS5147.1"
                     /db_xref="GeneID:9439"
                     /db_xref="HGNC:HGNC:2372"
                     /db_xref="MIM:605042"
                     /translation="METQLQSIFEEVVKTEVIEEAFPGMFMDTPEDEKTKLISCLGAF
                     RQFWGGLSQESHEQCIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLI
                     NSDTLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQL
                     LAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARI
                     NSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQ
                     PYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLWQHL
                     SSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADF
                     LPVMKLFDLLYPEKEYIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHS
                     LRLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYGNGIMRIP
                     LPGTNCMASGSITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVET
                     YSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLL
                     SHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLSDPKTVLSAESEE
                     LNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPGPLQ
                     AFFKQNNVPQESRFNLKKNVEEEYRKWKSMSNENDIITHFSMQGSPPLFLCLLWKMLL
                     ETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWK
                     YNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQ
                     NDWHTKHMNYHKKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVCLRFLPVFDIVIH
                     RFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMHLRDRAFLKRKLVHAI
                     IGSLKDNRPQGWCLSDTYLKCAMNAREENPWVPDDTYYCRLIGRLVDTMAGKSPGPFP
                     NCDWRFNEFPNPAAHALHVTCVELMALAVSGKEVGNALLNVVLKSQPLVPRENITAWM
                     NAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRLFDFTACHQSYSEMS
                     CSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHLVGPFLQRFQQE
                     RTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICN
                     LKPALKLRLRFITHISKMEPAAVPPQAMNSGSPAPQSNQVPVSLPVTQ"
     exon            233..264
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            265..352
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            353..477
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            478..589
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            590..688
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            689..790
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            791..860
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            861..973
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            974..1069
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            1070..1270
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            1271..1414
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            1415..1560
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            1561..1806
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            1807..1949
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            1950..2124
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            2125..2288
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            2289..2413
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            2414..2619
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            2620..2800
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            2801..2971
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            2972..3188
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            3189..3425
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            3426..3591
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            3592..3664
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            3665..3879
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            3880..4000
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            4001..4132
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            4133..5247
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5205..5210
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /note="hexamer: AGTAAA"
     regulatory      5223..5228
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /note="hexamer: AATAAA"
     polyA_site      5224
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
                     /note="major polyA site"
     polyA_site      5247
                     /gene="MED23"
                     /gene_synonym="ARC130; CRSP130; CRSP133; CRSP3; DRIP130;
                     MRT18; SUR-2; SUR2"
ORIGIN      
        1 gtcttccgta caggttcctg ctgttggctg ggctcgggag ggaacgctcc aggtaaagcg
       61 ctgggggagg aagcgacgcc gaggagctac ggtttcctcc agaggtctcc gcccctctgc
      121 ccctatattc ccagagctcg agtctgatcc gggccttgcc gggcaccctg gaaaggcggg
      181 ggtgatagta cagatggaga cgcaactgca gagcattttc gaagaggtgg tgaaaacgga
      241 agttatagaa gaggcttttc ctggcatgtt tatggatact cctgaagatg agaaaacaaa
      301 actaattagc tgtttggggg ccttcagaca gttttggggt ggtctttctc aggagtctca
      361 tgaacagtgt atccagtgga ttgttaagtt tattcatggt cagcatagtc ctaaaagaat
      421 ttcttttctt tatgactgct tagcaatggc agttgagact ggtctccttc cacccaggct
      481 ggtttgtgaa tccctgataa actctgacac tcttgagtgg gaaagaacac agctttgggc
      541 cttaacattt aaactggttc ggaaaataat tgggggagtg gattacaagg gtgttcgaga
      601 tctcttaaaa gtgattttgg agaagatttt gacaattcct aatacagtga gctctgctgt
      661 tgtacagcag cttctggcag caagagaggt tatagcatat atcttggaaa gaaatgcctg
      721 cttattacca gcctattttg cagtcactga gatcaggaaa ctgtatcctg aaggcaaact
      781 tccacactgg ttacttggaa acctagtatc agactttgtg gataccttca ggcccacagc
      841 aaggataaac tccatttgtg gtcgctgtag tcttctgcca gttgtaaata attcgggtgc
      901 catttgtaat tcatggaaac tggatcctgc tactcttcgt tttcctttga aaggcctttt
      961 gccatatgat aaggatctgt ttgaaccaca gactgctttg ttgagatatg tattggagca
     1021 gccttattcc agggatatgg tctgcaatat gctaggttta aataagcagc acaagcagcg
     1081 ctgccctgtg ctggaggacc agttggtgga tctggttgtt tatgccatgg agcgatctga
     1141 gaccgaggag aagtttgacg atgggggaac aagccaactc ctgtggcagc atctctcaag
     1201 tcagctcatt ttctttgtgc ttttccagtt tgcaagtttt ccacatatgg tgctttctct
     1261 tcatcagaag ttagcagggc gaggactgat taaaggcaga gatcatctta tgtgggttct
     1321 cctgcaattc atttctggaa gtattcagaa aaatgcacta gctgattttc tccctgtgat
     1381 gaagctcttc gacttgctat acccagaaaa agaatatatc ccagttcctg atattaacaa
     1441 accccagtca acccatgcct ttgcaatgac ctgtatttgg attcatctca atagaaaagc
     1501 tcaaaatgac aactccaagc tacagattcc aatacctcat tccctaagac ttcaccatga
     1561 gttcctgcag cagagtctaa gaaataaaag tttacagatg aatgactata agattgctct
     1621 attgtgtaat gcatactcta caaattcaga atgttttaca ttacccatgg gagctctggt
     1681 agaaactatt tatggaaatg gaattatgag gatacctctc cctggaacaa actgtatggc
     1741 ttcaggatct attaccccct tacctatgaa cctcctggat tcactgacag ttcatgccaa
     1801 aatgagcctt attcacagca ttgcaaccag ggtgataaaa cttgctcatg caaagtccag
     1861 tgtggccttg gctccagccc tagtggaaac ttacagtcgt ttattggtct atatggaaat
     1921 agagtctttg ggcatcaaag gatttatcag tcagcttttg ccaactgttt tcaaatcaca
     1981 tgcatggggg atcttacaca cactccttga gatgtttagc taccggatgc atcatattca
     2041 gcctcattac agagttcagc tcctgagtca tcttcatact ttggctgcag ttgcacaaac
     2101 aaaccagaac cagctccatc tttgtgtcga gagcactgct ctcaggctta taacagcatt
     2161 aggtagctca gaggtacaac cgcagtttac acgcttcctt agtgatccca aaacagtgct
     2221 ctcagcagaa tctgaagaac tgaaccgagc cttgatattg accttggcta gagcaactca
     2281 tgtaacagat ttttttacag gctctgattc aattcaggga acttggtgta aagacatact
     2341 tcagaccatc atgagtttca ctcctcataa ttgggcttca cacaccctga gctgttttcc
     2401 aggcccacta caggcattct tcaaacaaaa taatgtgcct caggaaagcc gttttaatct
     2461 gaaaaaaaat gtggaggagg agtataggaa gtggaagtca atgagcaacg aaaacgacat
     2521 tattacccac ttctctatgc agggctcccc tcctctcttt ctttgtcttc tctggaaaat
     2581 gctcttggaa acagatcata ttaatcagat tggctataga gtattagaga gaattggagc
     2641 cagggccttg gtagcccatg tgaggacatt tgcagatttc ctggtatatg agttttctac
     2701 atcagcaggg ggtcagcaac tcaataaatg cattgaaatt cttaatgaca tggtatggaa
     2761 gtataacatt gttacactgg acagattaat tctctgcctg gccatgcgta gtcacgaagg
     2821 aaatgaagcc caggtttgtt atttcataat tcagttgctg ttactcaaac caaacgattt
     2881 tagaaatcga gtaagtgact ttgtgaagga aaattcccca gagcactggt tacagaatga
     2941 ctggcacacc aagcacatga attatcacaa gaaatatcca gagaagttgt attttgaggg
     3001 cctcgcggaa caggtggatc ctcctgtaca gatccagtct ccctatctgc ccatctattt
     3061 tgggaatgtg tgtcttcgat tccttccagt atttgatata gtaatccaca gatttttaga
     3121 gttgcttccg gtatccaaat cactggagac tctactggat catctaggag gcttatataa
     3181 atttcatgat cgtccagtga cttatctgta taacactctg cactattatg aaatgcacct
     3241 gagagaccgc gcatttctca aacgaaaact cgtccatgcg atcattggct ctctgaagga
     3301 taatcgaccg cagggctggt gtctaagtga cacttacctg aaatgcgcta tgaatgcacg
     3361 agaggaaaat ccttgggttc cagatgacac ctactattgc agattgattg gcagactagt
     3421 cgatacgatg gctggcaaat ctcctggtcc ctttccaaac tgtgactgga gattcaatga
     3481 gtttcccaac ccagctgccc atgctctcca tgttacttgt gtggagctca tggccttggc
     3541 agtttcaggc aaagaagttg ggaatgccct tctaaatgtt gtcctaaaaa gtcagccttt
     3601 agtgccaaga gagaacatta cagcatggat gaatgcaatt ggtttgatca tcactgccct
     3661 accagagcca tattggattg ttcttcatga tcgaattgtg agtgtcatca gcagccccag
     3721 cttgacgtct gaaacagagt gggttggcta tccattccgc ctctttgatt tcactgcctg
     3781 tcatcagtcc tactctgaga tgagttgtag ctatacgtta gctcttgcac atgctgtgtg
     3841 gcaccattct agcatcggac aactttctct cattccaaag tttcttactg aagtacttct
     3901 tcctatagtg aagaccgaat tccagttgct ttatgtatac catcttgttg gaccattttt
     3961 acaaagattt cagcaagaga gaactcgttg tatgatagag attggtgtgg cgttttatga
     4021 catgctgctg aatgttgacc agtgtagcac ccatttaaat tacatggatc ccatctgtga
     4081 cttcctctat cacatgaagt atatgtttac tggtgacagc gtgaaagagc aagtagagaa
     4141 gattatctgt aacttaaaac cagctttaaa acttcgtctt cgattcatca cacacattag
     4201 caagatggag ccagctgcag tgcctccaca agccatgaac agtgggtctc cagcacctca
     4261 gtctaatcag gtgcccgtgt ctttaccagt aactcagtga agccagactg tactgtggag
     4321 aaagtagaaa tatatccgtc tttgagagtg gactcaaacc ttttaaatct gaatgcgatc
     4381 acactgtagt gatatagtag aagcagtgat gatattcaga ttgttttatt ttgattcaaa
     4441 tgatgaatct gttgacacct tataactccc atctccctat ctatattttt gtctctaaga
     4501 gatcaagtaa gatacatgtt gttcatattc ataacaactt attagagcta tgtaggaaca
     4561 tagaggtatc tgtgatcaaa tgacttgtca ttggcacttt cagtctttcc tattctagcc
     4621 gttgtttttg tttgtatttt ttattattaa tgacatattt agtcttcaga tatattattt
     4681 taaaggcacc acacatgtaa aacagttatt acaaatattt tatacataaa tctcttggta
     4741 gattttcaga ataatagaga tttatatcaa gaaaaggaat ctagattttg ttgcattgta
     4801 ggagcaaaca aggcttcata ttctttttaa aatgacttga aattttatta taaatttcca
     4861 tagtttctat caaggtcaac tggcagtttt attacctttg ctatggactt gaacttactg
     4921 tataaaatta gttggaagcc atgtaagaaa ataacatctg aaaattcttg aacttaaaat
     4981 catgcttaaa tggttgattc tccaatttct ttttttaaaa ataagttaat tgcttttgca
     5041 ggttgacact ctcacctgac agatgatgta attcttcaat ttttataatc ttaaaatttt
     5101 taaattttat atttgtaaat acagtacaca ttttatttct tggattttga gagacattgt
     5161 taattttggg ggaattggca ttgcgaaaga cttgaaaact aatgagtaaa gtctgctgaa
     5221 tgaataaacc gtgtgattgt ttcccga
//
