LOCUS       NM_133640               4044 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens mediator complex subunit 22 (MED22), transcript
            variant b, mRNA.
ACCESSION   NM_133640
VERSION     NM_133640.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 4044)
  AUTHORS   Rodriguez,P.Q., Unnersjo-Jess,D., Zambrano,S.S., Guo,J.,
            Moller-Hackbarth,K., Blom,H., Jahnukainen,T., Ebarasi,L. and
            Patrakka,J.
  TITLE     Inactivation of mediator complex protein 22 in podocytes results in
            intracellular vacuole formation, podocyte loss and premature death
  JOURNAL   Sci Rep 10 (1), 20037 (2020)
   PUBMED   33208756
  REMARK    GeneRIF: Inactivation of mediator complex protein 22 in podocytes
            results in intracellular vacuole formation, podocyte loss and
            premature death.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4044)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 4044)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 4044)
  AUTHORS   Angiolillo,A., Russo,G., Porcellini,A., Smaldone,S.,
            D'Alessandro,F. and Pietropaolo,C.
  TITLE     The human homologue of the mouse Surf5 gene encodes multiple
            alternatively spliced transcripts
  JOURNAL   Gene 284 (1-2), 169-178 (2002)
   PUBMED   11891058
  REMARK    GeneRIF: Hu-Surf5 encodes three different transcripts, Surf-5a,
            Surf-5b, and Surf-5c, which result from alternative splicing.
REFERENCE   7  (bases 1 to 4044)
  AUTHORS   Duhig,T., Ruhrberg,C., Mor,O. and Fried,M.
  TITLE     The human Surfeit locus
  JOURNAL   Genomics 52 (1), 72-78 (1998)
   PUBMED   9740673
REFERENCE   8  (bases 1 to 4044)
  AUTHORS   Garson,K., Duhig,T. and Fried,M.
  TITLE     Tissue-specific processing of the Surf-5 and Surf-4 mRNAs
  JOURNAL   Gene Expr 6 (4), 209-218 (1996)
   PUBMED   9196076
REFERENCE   9  (bases 1 to 4044)
  AUTHORS   Garson,K., Duhig,T., Armes,N., Colombo,P. and Fried,M.
  TITLE     Surf5: a gene in the tightly clustered mouse surfeit locus is
            highly conserved and transcribed divergently from the rpL7A (Surf3)
            gene
  JOURNAL   Genomics 30 (2), 163-170 (1995)
   PUBMED   8586415
  REMARK    GeneRIF: This publication also contains human sequence.
REFERENCE   10 (bases 1 to 4044)
  AUTHORS   Yon,J., Jones,T., Garson,K., Sheer,D. and Fried,M.
  TITLE     The organization and conservation of the human Surfeit gene cluster
            and its localization telomeric to the c-abl and can proto-oncogenes
            at chromosome band 9q34.1
  JOURNAL   Hum Mol Genet 2 (3), 237-240 (1993)
   PUBMED   8499913
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY154869.1, AJ224359.1,
            GD160955.1 and AL772161.10.
            
            On May 17, 2019 this sequence version replaced NM_133640.4.
            
            Summary: This gene encodes a protein component of the mediator
            complex, which functions in the regulation of transcription by
            bridging interactions between gene-specific regulatory factors, RNA
            polymerase II, and general transcription factors. Alternatively
            spliced transcript variants encoding different isoforms have been
            observed. [provided by RefSeq, Jul 2013].
            
            Transcript Variant: This variant (b) represents the shorter
            transcript and encodes the longer isoform (b).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ224359.1, SRR14038193.370109.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000343730.10/ ENSP00000342343.5
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-245               HY154869.1         26-270
            246-1451            AJ224359.1         216-1421
            1452-1455           GD160955.1         80-83
            1456-4044           AL772161.10        137309-139897       c
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q34.2"
     gene            1..4044
                     /gene="MED22"
                     /gene_synonym="MED24; SRB6; surf-5; SURF5"
                     /note="mediator complex subunit 22"
                     /db_xref="GeneID:6837"
                     /db_xref="HGNC:HGNC:11477"
                     /db_xref="MIM:185641"
     exon            1..210
                     /gene="MED22"
                     /gene_synonym="MED24; SRB6; surf-5; SURF5"
                     /inference="alignment:Splign:2.1.0"
     exon            211..371
                     /gene="MED22"
                     /gene_synonym="MED24; SRB6; surf-5; SURF5"
                     /inference="alignment:Splign:2.1.0"
     CDS             249..851
                     /gene="MED22"
                     /gene_synonym="MED24; SRB6; surf-5; SURF5"
                     /note="isoform b is encoded by transcript variant b;
                     surfeit locus protein 5; mediator of RNA polymerase II
                     transcription subunit 22"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 22 isoform b"
                     /protein_id="NP_598395.1"
                     /db_xref="CCDS:CCDS6963.1"
                     /db_xref="GeneID:6837"
                     /db_xref="HGNC:HGNC:11477"
                     /db_xref="MIM:185641"
                     /translation="MAQQRALPQSKETLLQSYNKRLKDDIKSIMDNFTEIIKTAKIED
                     ETQVSRATQGEQDNYEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAIDQRN
                     QQLRTLQEECDRKLITLRDEISIDLYELEEEYYSSSSSLCEANDLPLCEAYGRLDLDT
                     DSADGLSAPLLASPEPSAGPLQVAAPAHSHAGGPGPTEHA"
     exon            372..452
                     /gene="MED22"
                     /gene_synonym="MED24; SRB6; surf-5; SURF5"
                     /inference="alignment:Splign:2.1.0"
     exon            453..661
                     /gene="MED22"
                     /gene_synonym="MED24; SRB6; surf-5; SURF5"
                     /inference="alignment:Splign:2.1.0"
     exon            662..4044
                     /gene="MED22"
                     /gene_synonym="MED24; SRB6; surf-5; SURF5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1427..1432
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED22"
                     /gene_synonym="MED24; SRB6; surf-5; SURF5"
                     /note="hexamer: ATTAAA"
     polyA_site      1451
                     /gene="MED22"
                     /gene_synonym="MED24; SRB6; surf-5; SURF5"
                     /note="major polyA site"
     polyA_site      2453
                     /gene="MED22"
                     /gene_synonym="MED24; SRB6; surf-5; SURF5"
     polyA_site      2844
                     /gene="MED22"
                     /gene_synonym="MED24; SRB6; surf-5; SURF5"
ORIGIN      
        1 attcctataa agctaaaaat cgaggcgggg ccgagaggct gcggcggctg actaggtttt
       61 cggaccgcgg cgcggcgggg aagagagact gcggcgggag ggccaggccg tgggagagac
      121 gcgggaaagt gcgggtcgca gaggcacttg accaccccgc cctaggccga cccggcggac
      181 gcggcgtctg gtgtgcgggc gctggggcgg gtcccaggtc tccccgctgc gctgcttgag
      241 gctcggccat ggcccagcag agagccctgc cccagagcaa ggagacgctg ctgcagtcct
      301 acaacaagcg gctgaaggac gacattaagt ccatcatgga caacttcacc gagatcatca
      361 agaccgccaa gattgaggac gagacgcagg tgtcacgggc cactcagggt gaacaggaca
      421 attacgagat gcatgtgcga gccgccaaca tcgtccgagc cggcgagtcc ctgatgaagc
      481 tggtgtccga cctcaagcag ttcctgatcc tcaatgactt cccctccgtg aacgaggcca
      541 ttgaccagcg caaccagcag ctgcgcacac tgcaggagga gtgcgaccgg aagctcatca
      601 cgctgcgaga cgagatctcc attgacctct acgagctgga ggaggagtat tactcgtcca
      661 gctcaagcct ttgcgaagct aatgacctgc ctctgtgcga agcttacggg aggctggacc
      721 tcgacacaga ctctgctgat ggcctctcgg cccctctgct ggcgtccccg gagcccagtg
      781 ctggccccct acaggtggca gcccctgccc actcccatgc tggtggccct ggccccactg
      841 agcacgcctg agcctccggg gccacgcttc gttctcagga acaaaacctg aggcagccct
      901 ttggatgccc tcacagcctt gcttctctca gcctaggttc ccatttgggg acttcaggac
      961 cccagagcca ctaggacttc cttgggaagc ccgttagccc agggtgggtc ccgccaggac
     1021 agtagggaaa cagttgtttc cctagccatt tccgaatagc ccatcattcc gagtcatcat
     1081 ctctgtttgc tgccttcctg gccagccagg tggaagaaag tttccaagct aggtctggcc
     1141 cgttggggat ctcagcagtg gggcaggagg gtgcctgatt tcggggagtc ctgacccgag
     1201 cctgttgtca gagttgggag gggctctgag cagtgttggg caggccgggt ctcccatccc
     1261 gaggccagcg ttcctgtgca gagccccatc cactggttct tgccctgagc cacatatgtc
     1321 tgtgccatgg gctgagtgcc acgacaggcc cgtgtgacag ctgctgccca cgcatgtgga
     1381 agctaggtgg gactcattcc taattctgcc gttgtaatga gacttgatta aaacaccgcc
     1441 acttttttgc attgctgctc tttcttcctc attccttgtc agtccaggac catccttggt
     1501 ctcccagcag ttgtccgagc agcagctcct cagctctgcc tggacagcct ggcccaaggt
     1561 cactctctcc tcattggcac ctggtaggtc cccagtattc agtgaatgga cctgctgcca
     1621 tcattgcaca tccaggcacc tgtgcctctg ctggcatctc atcctcactg ctaccagagc
     1681 cggtgctcct agtgccggta ttttagagag gagaggatgt ggacttagaa ggggtgaggt
     1741 gtaccacggc cacagagcta ggaagtgaag tggcaggaat cagaacttga acctgatgga
     1801 agtctagacc cagtgtcttt tggtgccagg ctcaccttag aaatgcagaa gtcacaacac
     1861 tgggcaggaa gtgtgggggg agcacagttc gtccacagga agtgtggggg agcaccccac
     1921 cccagttcct ccagcaccat ccatgtgctt catcttctca tgggggaggc catcatcttt
     1981 cccgatgtat gaatgaggtg acagcccagg atccagcctt ggggacaggt aagaacacag
     2041 ctgacccatc accacctgaa ccagagaacc ccacagccaa gcagaaggca ccagacagac
     2101 aggagcttga ggcccagtcc tggctctgga cctggcttct ggggtggcct agggaagttg
     2161 cttcccctct gagggagaat ttccccattg atacgtgtgg tgatctgttc ccgcactatt
     2221 ttagctgtgg aaatgccttg tacttaacca ctgaggaaga aaaagattac aaccagatgg
     2281 aagcatatat gaagcgagag cccggaagga actggccaga ctttgtggtg ggatcccact
     2341 taccctgttc ctaaaatcct gagcgataag acctgccatc agcttcattt tctgcttggc
     2401 caggaccatc attcccatgt gaaaatcaag ttatttctcc ttcttaaagc caagccgctc
     2461 tgctgacctt ttttcctctc cagctcatgg ccttggcagc agagctccac ggggaagcag
     2521 ctgataacca tttgcagttc tctcttgggc ctacgtcaga caggttttgt ctccatgact
     2581 ctagcaaaac tacacctatt aaggtcacca gtggcctcca cattgctaag ccccggccca
     2641 ttctcagtcc atgtaactct tttatcccaa gctttttatt ttgagggcag tggaactcat
     2701 ggaagtactt gtcagtttgc ctctctgagc acattctcct tcgtccatca ccacaaccag
     2761 aatggatgat gattgcatat cctctggtat ctagctgtat tcagatttct tcagttgttc
     2821 cccaaatagt ttttttaatg cctatttttt ttctttctag tccagaggtc ttttattttt
     2881 ttaacaccca cgatgccatg aattcatagg gaagaggttc cagcagctca ggctccttcc
     2941 cattggttct cacagtgtgc tgctctgggt ggagcaggct ggcgcttcag ttgaatccag
     3001 gtacctttct ctttggcttc cctctttttc tgatcatttt ccttcacgcg tttcaggaag
     3061 ctctctcggc tcttagagtg cttagtgtgc tgaatatgca cattcattct cttggcaaga
     3121 atcttgccct tacttgttta caacagtgcc aacagcatgc tggggaacac tgtagactct
     3181 cccagtctag ccatggtgac atttgtgggg cattcctttt tgaacagtac ccattccctt
     3241 gatatctaca atatcacctt tctcatcaat ttgcatatac ttggccaaag gaacaactgc
     3301 atgttttctg aaaggcctag agaacatata ttgggtgcct ctcctctttc cctttgtgtt
     3361 cgtcattttg gcgaattact ggaaggtggc ggttccagct gaaaggcttt tatgcctgtt
     3421 tttattgtgt gttgcatttg gttgttattt tggagtctta aaatctaaaa caggaccagg
     3481 tcaggcccag tggctcctgc tgtaatccca gcactttgag aagccaaagc gggtggatca
     3541 cttgtggtca gaagttttga gaccagcctg ggcaacatgg agaaaccccg tctctactaa
     3601 aaagtataga aatcggccgg gcgcggtggc ttacgcctgc aaccccagca ctttgggagg
     3661 ccaaggcggg cggatcacct gaggtcggga tttccagacc agcctgacca acatggagaa
     3721 accctgtctc tactaaaaag tatagaaatt ggccgggcgc ggtggctcac gcctgtaatc
     3781 ccagcacttt gggaggccga ggcgggcaga tcacctgagg tcgggagttc cagaccagcc
     3841 tgaccaacat ggagaaaccc tgtctctact aaaaatacaa aaattagccg ggcgtgctgg
     3901 tccatgccta taatcccagc tacttggtag gcggaggcag gagaatcgct tgaacccggg
     3961 aggcggaggt tgcagtgagc cgagatcggg ccactgcact ccagcttggg caacaagagc
     4021 gaaactccac ctcaaaaaaa aaaa
//
