LOCUS       NM_004264               2669 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens mediator complex subunit 21 (MED21), transcript
            variant 1, mRNA.
ACCESSION   NM_004264
VERSION     NM_004264.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 2669)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 2669)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 2669)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   6  (bases 1 to 2669)
  AUTHORS   Pan,G., Aso,T. and Greenblatt,J.
  TITLE     Interaction of elongation factors TFIIS and elongin A with a human
            RNA polymerase II holoenzyme capable of promoter-specific
            initiation and responsive to transcriptional activators
  JOURNAL   J Biol Chem 272 (39), 24563-24571 (1997)
   PUBMED   9305922
REFERENCE   7  (bases 1 to 2669)
  AUTHORS   Scully,R., Anderson,S.F., Chao,D.M., Wei,W., Ye,L., Young,R.A.,
            Livingston,D.M. and Parvin,J.D.
  TITLE     BRCA1 is a component of the RNA polymerase II holoenzyme
  JOURNAL   Proc Natl Acad Sci U S A 94 (11), 5605-5610 (1997)
   PUBMED   9159119
REFERENCE   8  (bases 1 to 2669)
  AUTHORS   Cujec,T.P., Cho,H., Maldonado,E., Meyer,J., Reinberg,D. and
            Peterlin,B.M.
  TITLE     The human immunodeficiency virus transactivator Tat interacts with
            the RNA polymerase II holoenzyme
  JOURNAL   Mol Cell Biol 17 (4), 1817-1823 (1997)
   PUBMED   9121429
REFERENCE   9  (bases 1 to 2669)
  AUTHORS   Maldonado,E., Shiekhattar,R., Sheldon,M., Cho,H., Drapkin,R.,
            Rickert,P., Lees,E., Anderson,C.W., Linn,S. and Reinberg,D.
  TITLE     A human RNA polymerase II complex associated with SRB and
            DNA-repair proteins
  JOURNAL   Nature 381 (6577), 86-89 (1996)
   PUBMED   8609996
  REMARK    Erratum:[Nature 1996 Nov 28;384(6607):384]
REFERENCE   10 (bases 1 to 2669)
  AUTHORS   Chao,D.M., Gadbois,E.L., Murray,P.J., Anderson,S.F., Sonu,M.S.,
            Parvin,J.D. and Young,R.A.
  TITLE     A mammalian SRB protein associated with an RNA polymerase II
            holoenzyme
  JOURNAL   Nature 380 (6569), 82-85 (1996)
   PUBMED   8598913
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC092747.10.
            
            On Nov 23, 2018 this sequence version replaced NM_004264.4.
            
            Summary: This gene encodes a member of the mediator complex subunit
            21 family. The encoded protein interacts with the human RNA
            polymerase II holoenzyme and is involved in transcriptional
            regulation of RNA polymerase II transcribed genes. A pseudogene of
            this gene is located on chromosome 8. Alternative splicing results
            in multiple transcript variants. [provided by RefSeq, Nov 2012].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.224775.1,
                                           SRR3476690.681190.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000282892.4/ ENSP00000282892.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-64                AC092747.10        153866-153929       c
            65-179              AC092747.10        149953-150067       c
            180-280             AC092747.10        149040-149140       c
            281-2669            AC092747.10        145814-148202       c
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p11.23"
     gene            1..2669
                     /gene="MED21"
                     /gene_synonym="hSrb7; SRB7; SURB7"
                     /note="mediator complex subunit 21"
                     /db_xref="GeneID:9412"
                     /db_xref="HGNC:HGNC:11473"
                     /db_xref="MIM:603800"
     exon            1..64
                     /gene="MED21"
                     /gene_synonym="hSrb7; SRB7; SURB7"
                     /inference="alignment:Splign:2.1.0"
     CDS             23..457
                     /gene="MED21"
                     /gene_synonym="hSrb7; SRB7; SURB7"
                     /note="isoform 1 is encoded by transcript variant 1;
                     mediator of RNA polymerase II transcription subunit 21;
                     RNAPII complex component SRB7; RNA polymerase II
                     holoenzyme component SRB7; SRB7 suppressor of RNA
                     polymerase B homolog"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 21 isoform 1"
                     /protein_id="NP_004255.2"
                     /db_xref="CCDS:CCDS8711.1"
                     /db_xref="GeneID:9412"
                     /db_xref="HGNC:HGNC:11473"
                     /db_xref="MIM:603800"
                     /translation="MADRLTQLQDAVNSLADQFCNAIGVLQQCGPPASFNNIQTAINK
                     DQPANPTEEYAQLFAALIARTAKDIDVLIDSLPSEESTAALQAASLYKLEEENHEAAT
                     CLEDVVYRGDMLLEKIQSALADIAQSQLKTRSGTHSQSLPDS"
     exon            65..179
                     /gene="MED21"
                     /gene_synonym="hSrb7; SRB7; SURB7"
                     /inference="alignment:Splign:2.1.0"
     exon            180..280
                     /gene="MED21"
                     /gene_synonym="hSrb7; SRB7; SURB7"
                     /inference="alignment:Splign:2.1.0"
     exon            281..2669
                     /gene="MED21"
                     /gene_synonym="hSrb7; SRB7; SURB7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1724..1729
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED21"
                     /gene_synonym="hSrb7; SRB7; SURB7"
                     /note="hexamer: ATTAAA"
     polyA_site      1746
                     /gene="MED21"
                     /gene_synonym="hSrb7; SRB7; SURB7"
                     /note="major polyA site"
     regulatory      2643..2648
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED21"
                     /gene_synonym="hSrb7; SRB7; SURB7"
                     /note="hexamer: ATTAAA"
     polyA_site      2669
                     /gene="MED21"
                     /gene_synonym="hSrb7; SRB7; SURB7"
ORIGIN      
        1 gtctgtttgc tgcggtagga acatggcgga tcggctcacg cagcttcagg acgctgtgaa
       61 ttcgcttgca gatcagtttt gtaatgccat tggagtattg cagcaatgtg gtcctcctgc
      121 ctctttcaat aatattcaga cagcaattaa caaagaccag ccagctaacc ctacagaaga
      181 gtatgcccag ctttttgcag cactgattgc acgaacagca aaagacattg atgttttgat
      241 agattcctta cccagtgaag aatctacagc tgctttacag gctgctagct tgtataagct
      301 agaagaagaa aaccatgaag ctgctacatg tctggaggat gttgtttatc gaggagacat
      361 gcttctggag aagatacaaa gcgcacttgc tgatattgca cagtcacagc tgaagacaag
      421 aagtggtacc catagccagt ctcttccaga ctcatagcat cagtggatac catgtggctg
      481 agaaaagaac tgtttgagtg ccattaagaa ttctgcatca gacttagata caagccttac
      541 caacaattac agaaacatta aacactatga cacattacct ttttagctat ttttaatagt
      601 cttctatttt cactcttgat aagcttataa aatcatgatt gaatcagctt taaagcatca
      661 taccatcatt ttttaactga gtgaaattat taaggcatgt aatacattaa tgaacataat
      721 ataaggaaac atatgtaaaa ttctgttatg acataattta tgtctccatt ttgttgtatt
      781 ggccagtact tttacaaatc aaaacatctc tcaagccaaa ggagaagaca gtaagaacag
      841 acataaggga cattttagtt tgggctagtg tcctgcctct tagaggtggc attgtgtaaa
      901 tctgagcttt gagcaagaaa gttttggaaa ttgtgttgct tttaagaaat agagttagtg
      961 tggctggata agaaagtcac atttatgcaa atgtttcttc tgctagaacc tcatacctgt
     1021 tctagttcat ctccacgttt attttaccaa gagatcctct gtcaagagaa tttcctggct
     1081 gttgtgaaag aatttttcta catcctgaac tatttttcct atttcttttc accttgcttt
     1141 ttttcatcct taatttgctt gtcatcacaa tgaagtatgt tttttgaatc atcaattctt
     1201 tctcattctc catttatctg aaggttcttt tgcccttatt aacctctcaa cttttattgc
     1261 tgtattctag tcagaccaga acatacttcc actacatcag ttacttggca tcatttcacc
     1321 tcagtttatt attgccaaat aataatttgt gtcagcattt tcaactatgg ttattcatcc
     1381 aacccttgga tctctttgag tctactgata atctccactg gaaaggtgga attgaaatgt
     1441 ggtccacatt ttaactagct atttcctggg gctgtatttt tcagaatatg ctgagctaca
     1501 tttgctgcaa tctgttgcta ttcagagttt aagtttcagg agaaaacagg aacaatagaa
     1561 cactctgcct gttatttttg ttgtaatcaa gcttttccac agttcttgaa aagtactatg
     1621 tttcaaattt caggaacacc agcgttagct gtaaaagttg cagcaattta ttggctagtc
     1681 atagaaaatt tttgaacttt taactgtatt ttaattgatg tttattaaaa acactttgct
     1741 atcagatatt tggcataaat ctgtactctt cattatagtt ttggggggag agaagattca
     1801 gtcagaaaac ttattcaaag tacctaagta ttataaagga gtcaaaaagg tacaaagaga
     1861 aaaggtcaag acatttttca aatgagggaa aactaacagg atttatcact agtaaacctg
     1921 ctctaaaaga attcaaggga agctttttaa aaagaaggga agttatagca gaaggaaact
     1981 tagaatggca ggaataaaga aggcataatg tatagggtaa atataataga cttctcttga
     2041 ggttttaaaa attacatttg ttatttgaaa gaaaaaaatt aacgttgttg tatgtgattc
     2101 tctgtagagg atatacagtt tttttttgtt gttcttgttt ctgttttttt aaggtgaagt
     2161 ctctgtcacc caagctgaag tgcagttctg tgatcatggc tcactgcagc ttcaccctgg
     2221 gttcaggtga tcctcccact tcagcctctt cagtaactgg gactacaggc atgtactacc
     2281 acgtccagct aatttttttt ttcttttttt tttagagatg gggtctcact ctgttgccca
     2341 ggctaatgcc aaactgctaa cctcaagata ttaatactat acagtagact ataagaaatt
     2401 aagtatttgt atcatcccta gagcgacaca gatttttccc taaaaaacta cagattattt
     2461 tcaaaagcat taataaatta aggtggaata ctaaaaaaga ttcaggtaac ccaggaacga
     2521 gaaacagaat aaaacaatag taaacctaaa ttttatttta tttcattaat acagcaataa
     2581 ttaaatgtca atgatctaag ctgatttaaa tctaaaaact ggataagaag ctgtggcttg
     2641 taattaaaaa tagaccttta ttcactaga
//
