LOCUS       NM_004275               2478 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens mediator complex subunit 20 (MED20), transcript
            variant 1, mRNA.
ACCESSION   NM_004275
VERSION     NM_004275.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 2478)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 2478)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2478)
  AUTHORS   Shyam,R., Vachali,P., Gorusupudi,A., Nelson,K. and Bernstein,P.S.
  TITLE     All three human scavenger receptor class B proteins can bind and
            transport all three macular xanthophyll carotenoids
  JOURNAL   Arch Biochem Biophys 634, 21-28 (2017)
   PUBMED   28947101
  REMARK    GeneRIF: Our analyses revealed no apparent differences in protein
            expression profiles of SRBs in central and peripheral regions of
            human donor tissues, indicating that carotenoid-binding proteins
            rather than transporters are likely to mediate selective
            accumulation of carotenoids into the macula.
REFERENCE   6  (bases 1 to 2478)
  AUTHORS   Sato,S., Tomomori-Sato,C., Parmely,T.J., Florens,L., Zybailov,B.,
            Swanson,S.K., Banks,C.A., Jin,J., Cai,Y., Washburn,M.P.,
            Conaway,J.W. and Conaway,R.C.
  TITLE     A set of consensus mammalian mediator subunits identified by
            multidimensional protein identification technology
  JOURNAL   Mol Cell 14 (5), 685-691 (2004)
   PUBMED   15175163
REFERENCE   7  (bases 1 to 2478)
  AUTHORS   Sato,S., Tomomori-Sato,C., Banks,C.A., Parmely,T.J., Sorokina,I.,
            Brower,C.S., Conaway,R.C. and Conaway,J.W.
  TITLE     A mammalian homolog of Drosophila melanogaster transcriptional
            coactivator intersex is a subunit of the mammalian Mediator complex
  JOURNAL   J Biol Chem 278 (50), 49671-49674 (2003)
   PUBMED   14576168
REFERENCE   8  (bases 1 to 2478)
  AUTHORS   Sato,S., Tomomori-Sato,C., Banks,C.A., Sorokina,I., Parmely,T.J.,
            Kong,S.E., Jin,J., Cai,Y., Lane,W.S., Brower,C.S., Conaway,R.C. and
            Conaway,J.W.
  TITLE     Identification of mammalian Mediator subunits with similarities to
            yeast Mediator subunits Srb5, Srb6, Med11, and Rox3
  JOURNAL   J Biol Chem 278 (17), 15123-15127 (2003)
   PUBMED   12584197
REFERENCE   9  (bases 1 to 2478)
  AUTHORS   Mittler,G., Kremmer,E., Timmers,H.T. and Meisterernst,M.
  TITLE     Novel critical role of a human Mediator complex for basal RNA
            polymerase II transcription
  JOURNAL   EMBO Rep 2 (9), 808-813 (2001)
   PUBMED   11559591
REFERENCE   10 (bases 1 to 2478)
  AUTHORS   Xiao,H., Tao,Y. and Roeder,R.G.
  TITLE     The human homologue of Drosophila TRF-proximal protein is
            associated with an RNA polymerase II-SRB complex
  JOURNAL   J Biol Chem 274 (7), 3937-3940 (1999)
   PUBMED   9933582
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC012618.1 and BC040950.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_004275.4.
            
            Summary: This gene encodes a component of the mediator complex
            (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional
            coactivator complex thought to be required for the expression of
            almost all genes. The mediator complex is recruited by
            transcriptional activators or nuclear receptors to induce gene
            expression, by interacting with RNA polymerase II and promoting the
            formation of a transcriptional pre-initiation complex. A mutation
            in this gene has been associated with a novel infantile-onset
            neurodegenerative movement disorder. Alternatively spliced
            transcript variants have been identified. [provided by RefSeq, Mar
            2015].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC019866.1, BC012618.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000265350.9/ ENSP00000265350.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1608              BC012618.1         5-1612
            1609-1609           BC040950.1         1553-1553
            1610-2478           BC012618.1         1614-2482
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p21.1"
     gene            1..2478
                     /gene="MED20"
                     /gene_synonym="PRO0213; SRB2; TRFP"
                     /note="mediator complex subunit 20"
                     /db_xref="GeneID:9477"
                     /db_xref="HGNC:HGNC:16840"
                     /db_xref="MIM:612915"
     exon            1..135
                     /gene="MED20"
                     /gene_synonym="PRO0213; SRB2; TRFP"
                     /inference="alignment:Splign:2.1.0"
     CDS             122..760
                     /gene="MED20"
                     /gene_synonym="PRO0213; SRB2; TRFP"
                     /note="isoform 1 is encoded by transcript variant 1;
                     mediator of RNA polymerase II transcription subunit 20;
                     Trf (TATA binding protein-related factor)-proximal
                     homolog"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 20 isoform 1"
                     /protein_id="NP_004266.2"
                     /db_xref="CCDS:CCDS4862.1"
                     /db_xref="GeneID:9477"
                     /db_xref="HGNC:HGNC:16840"
                     /db_xref="MIM:612915"
                     /translation="MGVTCVSQMPVAEGKSVQQTVELLTRKLEMLGAEKQGTFCVDCE
                     TYHTAASTLGSQGQTGKLMYVMHNSEYPLSCFALFENGPCLIADTNFDVLMVKLKGFF
                     QSAKASKIETRGTRYQYCDFLVKVGTVTMGPSARGISVEVEYGPCVVASDCWSLLLEF
                     LQSFLGSHTPGAPAVFGNRHDAVYGPADTMVQYMELFNKIRKQQQVPVAGIR"
     exon            136..290
                     /gene="MED20"
                     /gene_synonym="PRO0213; SRB2; TRFP"
                     /inference="alignment:Splign:2.1.0"
     exon            291..544
                     /gene="MED20"
                     /gene_synonym="PRO0213; SRB2; TRFP"
                     /inference="alignment:Splign:2.1.0"
     exon            545..2478
                     /gene="MED20"
                     /gene_synonym="PRO0213; SRB2; TRFP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2459..2464
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED20"
                     /gene_synonym="PRO0213; SRB2; TRFP"
                     /note="hexamer: AATAAA"
     polyA_site      2478
                     /gene="MED20"
                     /gene_synonym="PRO0213; SRB2; TRFP"
                     /note="major polyA site"
ORIGIN      
        1 gtatttccgg agtcccttct gagacagaag gttgtgttgg ggaactgaag gagtttctgt
       61 gggcggacag ggaaccctgc gtttctactg tgtgattctg ccaccttcct ggcccgacgc
      121 catgggagtg acttgtgtgt cccagatgcc tgtggccgag ggcaagagtg ttcagcaaac
      181 cgtagagctc cttacccgga aattggagat gcttggggca gagaagcaag gaacattttg
      241 tgtggactgt gagacttacc atacggccgc ctctaccctt ggcagccaag gtcagaccgg
      301 gaagctgatg tatgtgatgc acaactcaga gtacccattg agctgtttcg ccctctttga
      361 gaatggccct tgccttattg ctgacaccaa ctttgatgtg cttatggtga agctcaaggg
      421 ctttttccag agtgctaagg ccagcaagat tgagacccgg ggcaccaggt accagtactg
      481 tgacttcctg gtgaaggtgg gcacggtcac aatggggccc agtgcccggg gcatctctgt
      541 ggaggtggag tatggcccct gtgtggtagc tagtgactgc tggagtctgc tgctcgagtt
      601 cctacagagt tttctaggca gccacacacc aggggctccc gcagtgtttg ggaacagaca
      661 tgatgcggtc tacggcccag cagataccat ggtccagtac atggaactct tcaacaagat
      721 ccgcaagcag cagcaggtgc cggtggctgg gattcgttag tgatgagcag ctgccagctg
      781 agctctgtca ccaggggtac tccacaggag gagcaggtgc tgactttcag gtccctggac
      841 cccagaaacc cagggtagac atggactcta ctctccttct ctggttctca gctgtggctt
      901 ttgttctggg gctgagtccc ctccccaacc ccctgactct cacacatagc cccccatcag
      961 ctgttttact ccgtgcctta ctggatttgg cctgtcctca agaatggtaa ttatgaagag
     1021 tggagaagtt tggaccttgc ctcctttagg ggaaaggtag gacaggaatg tccctgcctg
     1081 gtactggtgg gggaaatagt ccgtatcccc aactattaag gatttgtccc aggccaatgg
     1141 tgaacatgct gcattttatg tttggattgt gctgtaataa gagcctcttc cttccctcag
     1201 aggatgtggc tgggcttcta tcctagagat ggagtagaag gcacaactgg tttgatagtt
     1261 actattttct gacctgtttg ctgaagtgat ttgcgaattg actttcctag ggtgttgcct
     1321 gagtcctttg aaatctcctt ctgacatctt tcccttctgt tgtgaaatat gttaagccac
     1381 aggccaaact gctataagat cgaagtttgt ttttttctca cctaataata gagaggtaat
     1441 cagatggtct agggcagatt taactctaca aggttatcag gaactttgtt tccttctgtc
     1501 ttattcagcc attcccagta tgttttctta cccataagat caaagcagac tcatcagctc
     1561 catatctgca ttccactggg gaaagagaga gagaaaagag cagattttaa aaaaaagata
     1621 tgactgagca ttgctcagat cactgtggcc acactttgct gaaagagatg ctgggacata
     1681 gaacctctta tctgggcagt catgtgctga gttaaaactc agggattctg tgactacaag
     1741 atgaaagaca atttggttgc tgagatgcag ttaatggctt ctgttacatg gagctcaatg
     1801 gacgtgccca ggaatgcttt tggctgttat tttgcagtta atacctcctg taactaaagc
     1861 atttgtttat gagttgactt gagagaaggg ctgatctcag agccgctttg agctaagttg
     1921 gattagtcac actaggaagt taattccaca cctttcgtct aagtctcagt attgaggcct
     1981 ctccagttct catgcaccct gatcttaggg ttagaatact tgaccctgat acctgcacca
     2041 tgcttcatgg ttcctgagct ctttctcctg tttcatttga gcctccaaac tacatatttg
     2101 gtcatattgc ctgcctaccc catgcctgct gcagaaatat tcatccaggt taaccttgat
     2161 atacacagag atggtcttgg agaattgtga atgtatgtac tgtattgtca tcaaggatac
     2221 tgtcccttat ttgaaggcat ctaaagagaa actgttttca gatccaagtg ctcagatcta
     2281 aagcctctgc aacaagtcag gtggtggtca tgtttccctt ctagttttgg ctgacaggaa
     2341 gctcagttca gtaccataac tacagaacct gtcatctgta ttttttgttc tcaccccgtt
     2401 tttgttattt tgtttctggt tttttatatt gaggtatgtt ttagatatag tttacaaaaa
     2461 taaaacgcac agatctta
//
