LOCUS       NM_001317078            1099 bp    mRNA    linear   PRI 22-SEP-2024
DEFINITION  Homo sapiens mediator complex subunit 19 (MED19), transcript
            variant 1, mRNA.
ACCESSION   NM_001317078 XM_005273821
VERSION     NM_001317078.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Ruoff,R., Weber,H., Wang,Y., Huang,H., Shapiro,E., Fenyo,D. and
            Garabedian,M.J.
  TITLE     MED19 encodes two unique protein isoforms that confer prostate
            cancer growth under low androgen through distinct gene expression
            programs
  JOURNAL   Sci Rep 13 (1), 18227 (2023)
   PUBMED   37880276
  REMARK    GeneRIF: MED19 encodes two unique protein isoforms that confer
            prostate cancer growth under low androgen through distinct gene
            expression programs.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 1099)
  AUTHORS   Wang,K., Wang,X., Fu,X., Sun,J., Zhao,L., He,H. and Fan,Y.
  TITLE     Lung cancer metastasis-related protein 1 promotes the transferring
            from advanced metastatic prostate cancer to castration-resistant
            prostate cancer by activating the glucocorticoid receptor alpha
            signal pathway
  JOURNAL   Bioengineered 13 (3), 5373-5385 (2022)
   PUBMED   35184651
  REMARK    GeneRIF: Lung cancer metastasis-related protein 1 promotes the
            transferring from advanced metastatic prostate cancer to
            castration-resistant prostate cancer by activating the
            glucocorticoid receptor alpha signal pathway.
REFERENCE   4  (bases 1 to 1099)
  AUTHORS   Zhang,Y., Qin,P., Tian,L., Yan,J. and Zhou,Y.
  TITLE     The role of mediator complex subunit 19 in human diseases
  JOURNAL   Exp Biol Med (Maywood) 246 (15), 1681-1687 (2021)
   PUBMED   34038190
  REMARK    GeneRIF: The role of mediator complex subunit 19 in human diseases.
            Review article
REFERENCE   5  (bases 1 to 1099)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 1099)
  AUTHORS   Tomomori-Sato,C., Sato,S., Parmely,T.J., Banks,C.A., Sorokina,I.,
            Florens,L., Zybailov,B., Washburn,M.P., Brower,C.S., Conaway,R.C.
            and Conaway,J.W.
  TITLE     A mammalian mediator subunit that shares properties with
            Saccharomyces cerevisiae mediator subunit Cse2
  JOURNAL   J Biol Chem 279 (7), 5846-5851 (2004)
   PUBMED   14638676
REFERENCE   7  (bases 1 to 1099)
  AUTHORS   Sato,S., Tomomori-Sato,C., Banks,C.A., Parmely,T.J., Sorokina,I.,
            Brower,C.S., Conaway,R.C. and Conaway,J.W.
  TITLE     A mammalian homolog of Drosophila melanogaster transcriptional
            coactivator intersex is a subunit of the mammalian Mediator complex
  JOURNAL   J Biol Chem 278 (50), 49671-49674 (2003)
   PUBMED   14576168
REFERENCE   8  (bases 1 to 1099)
  AUTHORS   Sato,S., Tomomori-Sato,C., Banks,C.A., Sorokina,I., Parmely,T.J.,
            Kong,S.E., Jin,J., Cai,Y., Lane,W.S., Brower,C.S., Conaway,R.C. and
            Conaway,J.W.
  TITLE     Identification of mammalian Mediator subunits with similarities to
            yeast Mediator subunits Srb5, Srb6, Med11, and Rox3
  JOURNAL   J Biol Chem 278 (17), 15123-15127 (2003)
   PUBMED   12584197
REFERENCE   9  (bases 1 to 1099)
  AUTHORS   Mittler,G., Kremmer,E., Timmers,H.T. and Meisterernst,M.
  TITLE     Novel critical role of a human Mediator complex for basal RNA
            polymerase II transcription
  JOURNAL   EMBO Rep 2 (9), 808-813 (2001)
   PUBMED   11559591
REFERENCE   10 (bases 1 to 1099)
  AUTHORS   Reddy,P.H., Stockburger,E., Gillevet,P. and Tagle,D.A.
  TITLE     Mapping and characterization of novel (CAG)n repeat cDNAs from
            adult human brain derived by the oligo capture method
  JOURNAL   Genomics 46 (2), 174-182 (1997)
   PUBMED   9417904
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP000662.4.
            
            On Jun 9, 2021 this sequence version replaced NM_001317078.3.
            
            Summary: The protein encoded by this gene is a subunit of the
            Mediator complex, which binds to gene-specific regulatory factors
            and provides support for the basal RNA polymerase II transcription
            machinery. This gene has been implicated in the growth of several
            types of cancer, and inhibition of its expression inhibits the
            growth and spread of these cancers. Two transcript variants
            encoding different isoforms have been found for this gene.
            [provided by RefSeq, Nov 2015].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BI549905.1, SRR14038197.541007.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000431606.5/ ENSP00000416227.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-253               AP000662.4         107442-107694       c
            254-510             AP000662.4         100452-100708       c
            511-607             AP000662.4         100198-100294       c
            608-702             AP000662.4         99781-99875         c
            703-1099            AP000662.4         99189-99585         c
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q12.1"
     gene            1..1099
                     /gene="MED19"
                     /gene_synonym="DT2P1G7; LCMR1; MED19AS"
                     /note="mediator complex subunit 19"
                     /db_xref="GeneID:219541"
                     /db_xref="HGNC:HGNC:29600"
                     /db_xref="MIM:612385"
     exon            1..253
                     /gene="MED19"
                     /gene_synonym="DT2P1G7; LCMR1; MED19AS"
                     /inference="alignment:Splign:2.1.0"
     CDS             37..771
                     /gene="MED19"
                     /gene_synonym="DT2P1G7; LCMR1; MED19AS"
                     /note="isoform 1 is encoded by transcript variant 1; lung
                     cancer metastasis-related protein 1; mediator of RNA
                     polymerase II transcription subunit 19; mediator of RNA
                     polymerase II transcription, subunit 19 homolog; putative
                     mediator subunit MED19AS protein"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 19 isoform 1"
                     /protein_id="NP_001304007.2"
                     /db_xref="CCDS:CCDS86203.2"
                     /db_xref="GeneID:219541"
                     /db_xref="HGNC:HGNC:29600"
                     /db_xref="MIM:612385"
                     /translation="MENFTALFGAQADPPPPPTALGFGPGKPPPPPPPPAGGGPGTAP
                     PPTAATAPPGADKSGAGCGPFYLMRELPGSTELTGSTNLITHYNLEQAYNKFCGKKVK
                     EKLSNFLPDLPGMIDLPGSHDNSSLRSLIEKPPILSSSFNPITGTMLAGFRLHTGPLP
                     EQCRLMHIQPPKKKNKHKHKQSRTQDPVPPETPSDSDHKKKKKKKEEDPERKRKKKEK
                     KKKKNRHSPDHPGMGSSQASSSSSLR"
     exon            254..510
                     /gene="MED19"
                     /gene_synonym="DT2P1G7; LCMR1; MED19AS"
                     /inference="alignment:Splign:2.1.0"
     exon            511..607
                     /gene="MED19"
                     /gene_synonym="DT2P1G7; LCMR1; MED19AS"
                     /inference="alignment:Splign:2.1.0"
     exon            608..702
                     /gene="MED19"
                     /gene_synonym="DT2P1G7; LCMR1; MED19AS"
                     /inference="alignment:Splign:2.1.0"
     exon            703..1099
                     /gene="MED19"
                     /gene_synonym="DT2P1G7; LCMR1; MED19AS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1081..1086
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED19"
                     /gene_synonym="DT2P1G7; LCMR1; MED19AS"
                     /note="hexamer: ATTACA"
     polyA_site      1099
                     /gene="MED19"
                     /gene_synonym="DT2P1G7; LCMR1; MED19AS"
                     /note="major polyA site"
ORIGIN      
        1 ggcagacacg gagacagcgc cggggcggag ggtacgatgg agaatttcac ggcactgttt
       61 ggggctcagg ctgacccacc accgccccca accgcactcg gcttcggacc aggaaagcct
      121 ccacccccgc caccgcctcc tgcgggcggg ggacccggca cggccccgcc tcccaccgcg
      181 gccacggctc ctcctggcgc ggacaagtca ggagctggct gtggcccttt ttacctcatg
      241 agggaactgc caggtagcac agagctgaca ggcagcacga atctgatcac acactacaac
      301 ttggaacaag cctataataa attctgtggg aagaaggtga aggagaagct aagtaacttc
      361 ctgcctgacc tgccagggat gattgatctg cctggttccc atgataacag cagcctccgc
      421 tctctcattg agaagccccc tattctcagt agctctttca atcctatcac agggaccatg
      481 ctggccggct tccgcctcca cactggcccg ttgccggagc agtgtcgtct gatgcatatt
      541 cagcctccca agaagaagaa taagcacaag cacaaacaga gccgtaccca ggatcctgtc
      601 cccccagaaa caccatctga ttcagatcac aagaagaaga aaaagaaaaa agaagaggat
      661 cctgaacgga aaaggaagaa gaaagagaag aagaaaaaga agaatcgaca tagtccagac
      721 cacccaggta tgggcagctc ccaggccagc agcagcagca gcctacgcta ataggaccac
      781 tggactcttt gccaggatgg cttttcctgc tgtactgaac ctgctgataa aagctgcctt
      841 ccaggctctt ggacactgcc ttgggagcat cctgcagctg ggacagaggc cagctcctgt
      901 tgggctcagt tgagactaag taaattagga gagagaggga catgcttttg taggctcatc
      961 ccagttggtt ttctcatgga catctcttcc tctcccagga agcttacaat tttcttctct
     1021 ctcttttgtg caatttgtct gatttaggac ttgttctgtg ttttctttaa aaaaaaaaaa
     1081 attacattta tcaaactgg
//
