LOCUS       NM_017638               1829 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens mediator complex subunit 18 (MED18), transcript
            variant 1, mRNA.
ACCESSION   NM_017638 XM_003403718
VERSION     NM_017638.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 1829)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 1829)
  AUTHORS   Xuan,Y. and Wang,Y.
  TITLE     Long non-coding RNA SNHG3 promotes progression of gastric cancer by
            regulating neighboring MED18 gene methylation
  JOURNAL   Cell Death Dis 10 (10), 694 (2019)
   PUBMED   31534128
  REMARK    GeneRIF: Long non-coding RNA SNHG3 promotes progression of gastric
            cancer by regulating neighboring MED18 gene methylation.
            Erratum:[Cell Death Dis. 2022 Mar 11;13(3):226. doi:
            10.1038/s41419-022-04698-9.. PMID: 35277490]
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1829)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1829)
  AUTHORS   Sato,S., Tomomori-Sato,C., Parmely,T.J., Florens,L., Zybailov,B.,
            Swanson,S.K., Banks,C.A., Jin,J., Cai,Y., Washburn,M.P.,
            Conaway,J.W. and Conaway,R.C.
  TITLE     A set of consensus mammalian mediator subunits identified by
            multidimensional protein identification technology
  JOURNAL   Mol Cell 14 (5), 685-691 (2004)
   PUBMED   15175163
REFERENCE   7  (bases 1 to 1829)
  AUTHORS   Tomomori-Sato,C., Sato,S., Parmely,T.J., Banks,C.A., Sorokina,I.,
            Florens,L., Zybailov,B., Washburn,M.P., Brower,C.S., Conaway,R.C.
            and Conaway,J.W.
  TITLE     A mammalian mediator subunit that shares properties with
            Saccharomyces cerevisiae mediator subunit Cse2
  JOURNAL   J Biol Chem 279 (7), 5846-5851 (2004)
   PUBMED   14638676
REFERENCE   8  (bases 1 to 1829)
  AUTHORS   Sato,S., Tomomori-Sato,C., Banks,C.A., Parmely,T.J., Sorokina,I.,
            Brower,C.S., Conaway,R.C. and Conaway,J.W.
  TITLE     A mammalian homolog of Drosophila melanogaster transcriptional
            coactivator intersex is a subunit of the mammalian Mediator complex
  JOURNAL   J Biol Chem 278 (50), 49671-49674 (2003)
   PUBMED   14576168
REFERENCE   9  (bases 1 to 1829)
  AUTHORS   Sato,S., Tomomori-Sato,C., Banks,C.A., Sorokina,I., Parmely,T.J.,
            Kong,S.E., Jin,J., Cai,Y., Lane,W.S., Brower,C.S., Conaway,R.C. and
            Conaway,J.W.
  TITLE     Identification of mammalian Mediator subunits with similarities to
            yeast Mediator subunits Srb5, Srb6, Med11, and Rox3
  JOURNAL   J Biol Chem 278 (17), 15123-15127 (2003)
   PUBMED   12584197
REFERENCE   10 (bases 1 to 1829)
  AUTHORS   Mittler,G., Kremmer,E., Timmers,H.T. and Meisterernst,M.
  TITLE     Novel critical role of a human Mediator complex for basal RNA
            polymerase II transcription
  JOURNAL   EMBO Rep 2 (9), 808-813 (2001)
   PUBMED   11559591
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK000052.1 and AL353622.33.
            
            On Nov 22, 2018 this sequence version replaced NM_017638.2.
            
            Summary: MED18 is a component of the Mediator complex, which is a
            coactivator for DNA-binding factors that activate transcription via
            RNA polymerase II (Sato et al., 2003 [PubMed 12584197]).[supplied
            by OMIM, Oct 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Variants 1 and 2 both encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.176063.1,
                                           SRR1803613.139732.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000373842.9/ ENSP00000362948.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-649               AK000052.1         3-651
            650-1829            AL353622.33        25635-26814         c
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p35.3"
     gene            1..1829
                     /gene="MED18"
                     /gene_synonym="p28b; SRB5"
                     /note="mediator complex subunit 18"
                     /db_xref="GeneID:54797"
                     /db_xref="HGNC:HGNC:25944"
                     /db_xref="MIM:612384"
     exon            1..141
                     /gene="MED18"
                     /gene_synonym="p28b; SRB5"
                     /inference="alignment:Splign:2.1.0"
     exon            142..280
                     /gene="MED18"
                     /gene_synonym="p28b; SRB5"
                     /inference="alignment:Splign:2.1.0"
     CDS             208..834
                     /gene="MED18"
                     /gene_synonym="p28b; SRB5"
                     /note="TRAP/mediator complex subunit p28b; mediator of RNA
                     polymerase II transcription, subunit 18 homolog"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 18"
                     /protein_id="NP_060108.2"
                     /db_xref="CCDS:CCDS322.1"
                     /db_xref="GeneID:54797"
                     /db_xref="HGNC:HGNC:25944"
                     /db_xref="MIM:612384"
                     /translation="MEAPPVTMMPVTGGTINMMEYLLQGSVLDHSLESLIHRLRGLCD
                     NMEPETFLDHEMVFLLKGQQASPFVLRARRSMDRAGAPWHLRYLGQPEMGDKNRHALV
                     RNCVDIATSENLTDFLMEMGFRMDHEFVAKGHLFRKGIMKIMVYKIFRILVPGNTDST
                     EALSLSYLVELSVVAPAGQDMVSDDMKNFAEQLKPLVHLEKIDPKRLM"
     exon            281..1829
                     /gene="MED18"
                     /gene_synonym="p28b; SRB5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1811..1816
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED18"
                     /gene_synonym="p28b; SRB5"
                     /note="hexamer: AATAAA"
     polyA_site      1829
                     /gene="MED18"
                     /gene_synonym="p28b; SRB5"
                     /note="major polyA site"
ORIGIN      
        1 agttggctgg ttgcaccgat ctgggggctt cccgggctcg ggtaaccgga gtgctggtat
       61 ctaatcgtcg ctcaaaagct cctaggtgcg cgggtggata taattcccgg cttctcgaga
      121 agacactaca gcctcaagga ggtatatccc gtgccttacc tgactggggg ctctgagtcc
      181 agttgtgttg tcttcaactt agacaccatg gaggcacctc cagtcaccat gatgcctgtc
      241 actgggggca ccattaacat gatggagtac ctgttgcagg gaagtgtttt agatcacagt
      301 ttggaaagcc tcatccaccg ccttcgtggt ttgtgtgaca acatggaacc tgagactttc
      361 cttgaccatg agatggtatt cctccttaag ggccagcaag ccagcccatt tgttctcagg
      421 gcccgacgct ctatggacag ggcaggggca ccctggcatc tgcgctacct gggacagcca
      481 gaaatgggag acaagaaccg ccatgccctg gtgcgaaact gcgtggacat tgccacatct
      541 gagaacctca ccgacttctt gatggaaatg ggcttccgca tggaccatga gtttgttgct
      601 aagggacatt tgttccgtaa gggcatcatg aagattatgg tgtacaagat tttccgcatc
      661 ctggtgccag ggaacacaga cagcactgag gccttgtcac tctcctatct cgtggaatta
      721 agtgtggtag cacccgctgg gcaggacatg gtctctgatg acatgaagaa cttcgcagaa
      781 cagctaaaac ctctggttca cctagagaaa atagacccca agaggctcat gtgactaaga
      841 ggatctgtcc acatttgggg cctatcctta cttgtttgaa aaaatatgtt tgcttttttt
      901 ggtttttgtt ttgttttgtt tttgagacag agtctcgctt tgtttcccag gctggagtgc
      961 agtggcacga tctcggctca ctgcaacctc tgcctcctgg gttcaagcaa ttctcccacc
     1021 tcagcctcct gagtagctgg gattacaggc acatgccacc atgctcagct aatttttgta
     1081 tttttagtag aaatggggat tcaccatgtt ggtcaggcta ttctcgaact cctgacctcg
     1141 tgatccaccc accttggcct cccaaagtgc tgggattaca ggcatgagcc accacgcctg
     1201 gccaaaaaaa tatgttttaa atgtcccatt tcaccattgc caggcaggca ttcttccttc
     1261 agggagagga tggtgagaat taattggttc tttgcactgt tctcctcatg tggcgatttc
     1321 actttcatga cagcctttct atattaaagg ctcaggatgt cacggagaat ctatctaatc
     1381 ccactgtatt aagaggggaa accgggccaa gcgcagtggc tcacacctct aatcccagca
     1441 ctttgggagg ctgaggtagg tggatcaccc aaggtcagga gtttgagacc agcctggcca
     1501 aatggtgaaa ccccatctct acgaaaaata caaaatttag ccgggcatgg tagcaggcgc
     1561 ttgtaatccc agctacttgg gaggctgagg ctggagaatc gcttgaaccc aggaggtgaa
     1621 ggttgcagtg accagagatg acgccattgc actccagcct gggtgagaag agcgaaactc
     1681 cgtctcaaaa aaaaataaaa atgaagaggg gaaaccagaa taaattatct tttggaaagg
     1741 acaatttctt gtttggccat ttgtgtataa ggttggtaac attagaggct gtgagcttgt
     1801 gttacatggt aataaagcca atgaagaga
//
