LOCUS       NM_005481               2892 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens mediator complex subunit 16 (MED16), mRNA.
ACCESSION   NM_005481 XM_939640
VERSION     NM_005481.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 2892)
  AUTHORS   Gao,H., Bai,P., Xiao,L., Shen,M., Yu,Q., Lei,Y., Huang,W., Lin,X.,
            Zheng,X., Wei,T., Jiang,Y., Ye,F. and Bu,H.
  TITLE     Mediator complex subunit 16 is down-regulated in papillary thyroid
            cancer, leading to increased transforming growth factor-beta
            signaling and radioiodine resistance
  JOURNAL   J Biol Chem 295 (31), 10726-10740 (2020)
   PUBMED   32532820
  REMARK    GeneRIF: Mediator complex subunit 16 is down-regulated in papillary
            thyroid cancer, leading to increased transforming growth
            factor-beta signaling and radioiodine resistance.
REFERENCE   4  (bases 1 to 2892)
  AUTHORS   Basel-Vanagaite,L., Smirin-Yosef,P., Essakow,J.L., Tzur,S.,
            Lagovsky,I., Maya,I., Pasmanik-Chor,M., Yeheskel,A., Konen,O.,
            Orenstein,N., Weisz Hubshman,M., Drasinover,V., Magal,N., Peretz
            Amit,G., Zalzstein,Y., Zeharia,A., Shohat,M., Straussberg,R.,
            Monte,D., Salmon-Divon,M. and Behar,D.M.
  TITLE     Homozygous MED25 mutation implicated in eye-intellectual disability
            syndrome
  JOURNAL   Hum Genet 134 (6), 577-587 (2015)
   PUBMED   25792360
REFERENCE   5  (bases 1 to 2892)
  AUTHORS   Lambert,J.P., Tucholska,M., Go,C., Knight,J.D. and Gingras,A.C.
  TITLE     Proximity biotinylation and affinity purification are complementary
            approaches for the interactome mapping of chromatin-associated
            protein complexes
  JOURNAL   J Proteomics 118, 81-94 (2015)
   PUBMED   25281560
REFERENCE   6  (bases 1 to 2892)
  AUTHORS   Mittler,G., Kremmer,E., Timmers,H.T. and Meisterernst,M.
  TITLE     Novel critical role of a human Mediator complex for basal RNA
            polymerase II transcription
  JOURNAL   EMBO Rep 2 (9), 808-813 (2001)
   PUBMED   11559591
REFERENCE   7  (bases 1 to 2892)
  AUTHORS   Suzuki,Y., Tsunoda,T., Sese,J., Taira,H., Mizushima-Sugano,J.,
            Hata,H., Ota,T., Isogai,T., Tanaka,T., Nakamura,Y., Suyama,A.,
            Sakaki,Y., Morishita,S., Okubo,K. and Sugano,S.
  TITLE     Identification and characterization of the potential promoter
            regions of 1031 kinds of human genes
  JOURNAL   Genome Res 11 (5), 677-684 (2001)
   PUBMED   11337467
REFERENCE   8  (bases 1 to 2892)
  AUTHORS   Rachez,C., Lemon,B.D., Suldan,Z., Bromleigh,V., Gamble,M.,
            Naar,A.M., Erdjument-Bromage,H., Tempst,P. and Freedman,L.P.
  TITLE     Ligand-dependent transcription activation by nuclear receptors
            requires the DRIP complex
  JOURNAL   Nature 398 (6730), 824-828 (1999)
   PUBMED   10235266
REFERENCE   9  (bases 1 to 2892)
  AUTHORS   Ito,M., Yuan,C.X., Malik,S., Gu,W., Fondell,J.D., Yamamura,S.,
            Fu,Z.Y., Zhang,X., Qin,J. and Roeder,R.G.
  TITLE     Identity between TRAP and SMCC complexes indicates novel pathways
            for the function of nuclear receptors and diverse mammalian
            activators
  JOURNAL   Mol Cell 3 (3), 361-370 (1999)
   PUBMED   10198638
REFERENCE   10 (bases 1 to 2892)
  AUTHORS   Fondell,J.D., Ge,H. and Roeder,R.G.
  TITLE     Ligand induction of a transcriptionally active thyroid hormone
            receptor coactivator complex
  JOURNAL   Proc Natl Acad Sci U S A 93 (16), 8329-8333 (1996)
   PUBMED   8710870
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BI461485.1, BC017282.2, CB050542.1, BQ015734.1 and AL137732.1.
            
            On Nov 23, 2018 this sequence version replaced NM_005481.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074967.2395317.1,
                                           SRR14038193.3030126.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000325464.6/ ENSP00000325612.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-368               BI461485.1         34-401
            369-2218            BC017282.2         375-2224
            2219-2519           BC017282.2         2282-2582
            2520-2739           CB050542.1         178-397             c
            2740-2804           BQ015734.1         107-171             c
            2805-2892           AL137732.1         1702-1789
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.3"
     gene            1..2892
                     /gene="MED16"
                     /gene_synonym="DRIP92; THRAP5; TRAP95"
                     /note="mediator complex subunit 16"
                     /db_xref="GeneID:10025"
                     /db_xref="HGNC:HGNC:17556"
                     /db_xref="MIM:604062"
     exon            1..102
                     /gene="MED16"
                     /gene_synonym="DRIP92; THRAP5; TRAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            103..289
                     /gene="MED16"
                     /gene_synonym="DRIP92; THRAP5; TRAP95"
                     /inference="alignment:Splign:2.1.0"
     CDS             121..2754
                     /gene="MED16"
                     /gene_synonym="DRIP92; THRAP5; TRAP95"
                     /note="thyroid hormone receptor-associated protein, 95-kD
                     subunit; thyroid hormone receptor-associated protein 5;
                     vitamin D3 receptor-interacting protein complex 92 kDa
                     component; thyroid hormone receptor-associated protein
                     complex 95 kDa component"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 16"
                     /protein_id="NP_005472.2"
                     /db_xref="CCDS:CCDS12047.1"
                     /db_xref="GeneID:10025"
                     /db_xref="HGNC:HGNC:17556"
                     /db_xref="MIM:604062"
                     /translation="MCDLRRPAAGGMMDLAYVCEWEKWSKSTHCPSVPLACAWSCRNL
                     IAFTMDLRSDDQDLTRMIHILDTEHPWDLHSIPSEHHEAITCLEWDQSGSRLLSADAD
                     GQIKCWSMADHLANSWESSVGSLVEGDPIVALSWLHNGVKLALHVEKSGASSFGEKFS
                     RVKFSPSLTLFGGKPMEGWIAVTVSGLVTVSLLKPSGQVLTSTESLCRLRGRVALADI
                     AFTGGGNIVVATADGSSASPVQFYKVCVSVVSEKCRIDTEILPSLFMRCTTDLNRKDK
                     FPAITHLKFLARDMSEQVLLCASSQTSSIVECWSLRKEGLPVNNIFQQISPVVGDKQP
                     TILKWRILSATNDLDRVSAVALPKLPISLTNTDLKVASDTQFYPGLGLALAFHDGSVH
                     IVHRLSLQTMAVFYSSAAPRPVDEPAMKRPRTAGPAVHLKAMQLSWTSLALVGIDSHG
                     KLSVLRLSPSMGHPLEVGLALRHLLFLLEYCMVTGYDWWDILLHVQPSMVQSLVEKLH
                     EEYTRQTAALQQVLSTRILAMKASLCKLSPCTVTRVCDYHTKLFLIAISSTLKSLLRP
                     HFLNTPDKSPGDRLTEICTKITDVDIDKVMINLKTEEFVLDMNTLQALQQLLQWVGDF
                     VLYLLASLPNQGSLLRPGHSFLRDGTSLGMLRELMVVIRIWGLLKPSCLPVYTATSDT
                     QDSMSLLFRLLTKLWICCRDEGPASEPDEALVDECCLLPSQLLIPSLDWLPASDGLVS
                     RLQPKQPLRLQFGRAPTLPGSAATLQLDGLARAPGQPKIDHLRRLHLGACPTEECKAC
                     TRCGCVTMLKSPNRTTAVKQWEQRWIKNCLAVEGRGPDACVTSRASEEAPAFVQLGPQ
                     STHHSPRTPRSLDHLHPEDRP"
     exon            290..397
                     /gene="MED16"
                     /gene_synonym="DRIP92; THRAP5; TRAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            398..567
                     /gene="MED16"
                     /gene_synonym="DRIP92; THRAP5; TRAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            568..999
                     /gene="MED16"
                     /gene_synonym="DRIP92; THRAP5; TRAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            1000..1105
                     /gene="MED16"
                     /gene_synonym="DRIP92; THRAP5; TRAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..1261
                     /gene="MED16"
                     /gene_synonym="DRIP92; THRAP5; TRAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            1262..1473
                     /gene="MED16"
                     /gene_synonym="DRIP92; THRAP5; TRAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            1474..1680
                     /gene="MED16"
                     /gene_synonym="DRIP92; THRAP5; TRAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            1681..1891
                     /gene="MED16"
                     /gene_synonym="DRIP92; THRAP5; TRAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            1892..2025
                     /gene="MED16"
                     /gene_synonym="DRIP92; THRAP5; TRAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            2026..2218
                     /gene="MED16"
                     /gene_synonym="DRIP92; THRAP5; TRAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            2219..2435
                     /gene="MED16"
                     /gene_synonym="DRIP92; THRAP5; TRAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            2436..2519
                     /gene="MED16"
                     /gene_synonym="DRIP92; THRAP5; TRAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            2520..2603
                     /gene="MED16"
                     /gene_synonym="DRIP92; THRAP5; TRAP95"
                     /inference="alignment:Splign:2.1.0"
     exon            2604..2892
                     /gene="MED16"
                     /gene_synonym="DRIP92; THRAP5; TRAP95"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2872..2877
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED16"
                     /gene_synonym="DRIP92; THRAP5; TRAP95"
                     /note="hexamer: AATAAA"
     polyA_site      2892
                     /gene="MED16"
                     /gene_synonym="DRIP92; THRAP5; TRAP95"
                     /note="major polyA site"
ORIGIN      
        1 aggcgactgg ttgttaccga ggaagatggc ggcgccagac ccgaggcgct agggaagatc
       61 gcaccgcgga cgcccgctga gcttggcgca cgggccgacc aggagctggt gactgccctc
      121 atgtgtgatt tgcggcggcc agcggcaggt gggatgatgg acttggccta cgtctgtgag
      181 tgggagaaat ggtccaagag cacccactgc ccatcggtgc ccctggcctg cgcctggtcc
      241 tgccgaaatc tcatcgcctt caccatggac ctgcgcagcg atgaccagga cctgacccgc
      301 atgatccaca tcctggacac ggagcacccc tgggacctgc actcgatccc ctcagagcac
      361 cacgaggcca tcacctgcct ggagtgggac cagtcaggct cccggctcct gtcagcagat
      421 gccgacgggc agatcaagtg ctggagcatg gcggaccacc tggctaatag ctgggagagc
      481 tcagtgggca gcctagtgga gggggacccc attgtggccc tgtcctggct gcacaatggt
      541 gtgaaactgg ccctgcacgt ggagaagtcg ggcgcctcca gcttcgggga gaagttctcc
      601 cgagtcaagt tctcaccgtc gctcacgctg ttcggcggca agcccatgga gggctggatc
      661 gcggtgacgg tcagcggcct ggtcaccgtg tccctgctga agcccagcgg gcaggtgctg
      721 acgtccaccg agagcctgtg ccggctgcgc ggccgcgtgg ccctggccga catcgccttc
      781 accggcggcg gcaacatcgt ggtggccacg gcggacggca gcagcgcgtc gcccgtgcag
      841 ttctacaagg tgtgcgtgag cgtggtgagc gagaagtgcc gtatcgacac ggagatcctg
      901 ccctccctgt tcatgcgctg caccaccgac ctcaaccgca aggacaagtt tcccgccatc
      961 acccacctca agttcctggc ccgggacatg tcggagcagg tgcttttgtg cgcgtccagc
     1021 cagaccagca gcatcgtgga gtgctggtcc ctgcgcaagg agggactccc cgtgaacaac
     1081 atcttccagc agatctcccc cgtggttggc gacaaacagc ccacaattct caaatggcgg
     1141 atcctatcgg ccaccaacga tctggaccgt gtgtcggccg tggcgctgcc caagctgccc
     1201 atctcgctca ccaacaccga cctcaaggtg gccagcgaca cacagttcta ccctggcctc
     1261 gggctggccc tggccttcca cgacggcagc gtccacatcg tgcaccggct ctcactgcag
     1321 accatggccg tcttctacag ctccgcggcc ccgaggcctg tggatgagcc ggccatgaag
     1381 cgcccccgca ccgcgggccc cgccgtccac ttaaaggcta tgcagctatc gtggacgtca
     1441 ctggccctgg tggggattga cagccacggg aagctgagcg tgctccgcct ctcaccttcc
     1501 atgggccacc cgctggaggt ggggctggcg ctgcggcacc tgctcttcct gctggagtac
     1561 tgcatggtga ccggctacga ctggtgggac atcctgctgc acgtgcagcc cagtatggta
     1621 cagagcctgg tggagaagct gcacgaggag tacacgcgcc agaccgctgc cctgcagcag
     1681 gtcctctcca cccggatcct ggccatgaag gcctcgctct gcaagctgtc gccctgcacg
     1741 gtgacccgcg tgtgcgacta ccacaccaag ctcttcctca tcgccatcag ctccaccctg
     1801 aagtcgctgc tgcgccccca ctttctcaac acgcctgaca agagccccgg cgaccggctg
     1861 accgagatct gcaccaagat caccgacgtc gacattgaca aggtcatgat caacctcaag
     1921 acggaggaat ttgtgctgga catgaacaca ctgcaggcgc tgcagcagct cttgcagtgg
     1981 gtgggcgact tcgtgctgta cctgctggcc agcctaccca accagggttc cctgctgagg
     2041 ccgggccaca gctttctgcg ggacggcacc tcgctgggca tgcttcggga attgatggtg
     2101 gtcatccgca tctggggcct tctgaagccc agctgcctgc ccgtgtatac ggccacctcg
     2161 gatacccagg acagcatgtc cctgctcttc cgcctgctca ccaagctctg gatctgctgt
     2221 cgcgatgagg gcccagcgag cgagccggac gaggcgctgg tggatgaatg ctgcctgctg
     2281 cccagccagc tgcttatccc cagcctggac tggctgccag ccagcgacgg cctggttagc
     2341 cgcctgcagc ccaagcagcc ccttcgtctg cagtttggcc gggcgcccac gctgcctggc
     2401 agtgctgcca ccctgcagct cgacggcctc gccagggccc caggccagcc caagatcgac
     2461 cacctgcgga ggctgcacct tggcgcttgc cccacggagg aatgcaaggc ctgcaccagg
     2521 tgcggctgtg tcaccatgct caagtcgccc aacagaacca cggcggtgaa gcagtgggag
     2581 cagcgctgga tcaagaactg cctggctgtt gaaggccgtg ggccggacgc ctgcgtgacc
     2641 agcagagctt ctgaggaagc ccctgccttt gtccagctgg gcccgcagtc cacacaccac
     2701 tctcccagga cccccagatc cctggaccat ctgcatccag aggaccgtcc gtgacggccg
     2761 ggggtccagg cggaccttgt ggtgacccgg ctcgggcgtc tcctcggttt ccttgcctca
     2821 cccgcggaga gcgctgaacc tggacaagca gcggctggga aggacaggtc caataaacgc
     2881 cctctgcgcc ca
//
