LOCUS       NM_001003891            3351 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens mediator complex subunit 15 (MED15), transcript
            variant 1, mRNA.
ACCESSION   NM_001003891
VERSION     NM_001003891.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Doytcheva,K., Gallan,A.J., Wang,P., Wanjari,P., Segal,J. and
            Antic,T.
  TITLE     Cystic MED15::TFE3 translocation renal cell carcinoma: histologic
            mimicker of multilocular cystic renal neoplasm of low malignant
            potential with review of the literature
  JOURNAL   Hum Pathol 136, 25-33 (2023)
   PUBMED   36997032
  REMARK    GeneRIF: Cystic MED15::TFE3 translocation renal cell carcinoma:
            histologic mimicker of multilocular cystic renal neoplasm of low
            malignant potential with review of the literature.
            Review article
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 3351)
  AUTHORS   Sanborn,A.L., Yeh,B.T., Feigerle,J.T., Hao,C.V., Townshend,R.J.,
            Lieberman Aiden,E., Dror,R.O. and Kornberg,R.D.
  TITLE     Simple biochemical features underlie transcriptional activation
            domain diversity and dynamic, fuzzy binding to Mediator
  JOURNAL   Elife 10, e68068 (2021)
   PUBMED   33904398
  REMARK    GeneRIF: Simple biochemical features underlie transcriptional
            activation domain diversity and dynamic, fuzzy binding to Mediator.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3351)
  AUTHORS   Batlle,C., Calvo,I., Iglesias,V., J Lynch,C., Gil-Garcia,M.,
            Serrano,M. and Ventura,S.
  TITLE     MED15 prion-like domain forms a coiled-coil responsible for its
            amyloid conversion and propagation
  JOURNAL   Commun Biol 4 (1), 414 (2021)
   PUBMED   33772081
  REMARK    GeneRIF: MED15 prion-like domain forms a coiled-coil responsible
            for its amyloid conversion and propagation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3351)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   6  (bases 1 to 3351)
  AUTHORS   Mittler,G., Kremmer,E., Timmers,H.T. and Meisterernst,M.
  TITLE     Novel critical role of a human Mediator complex for basal RNA
            polymerase II transcription
  JOURNAL   EMBO Rep 2 (9), 808-813 (2001)
   PUBMED   11559591
REFERENCE   7  (bases 1 to 3351)
  AUTHORS   Berti,L., Mittler,G., Przemeck,G.K., Stelzer,G., Gunzler,B.,
            Amati,F., Conti,E., Dallapiccola,B., Hrabe de Angelis,M.,
            Novelli,G. and Meisterernst,M.
  TITLE     Isolation and characterization of a novel gene from the DiGeorge
            chromosomal region that encodes for a mediator subunit
  JOURNAL   Genomics 74 (3), 320-332 (2001)
   PUBMED   11414760
REFERENCE   8  (bases 1 to 3351)
  AUTHORS   Abraham,S. and Solomon,W.B.
  TITLE     A novel glutamine-rich putative transcriptional adaptor protein
            (TIG-1), preferentially expressed in placental and bone-marrow
            tissues
  JOURNAL   Gene 255 (2), 389-400 (2000)
   PUBMED   11024300
REFERENCE   9  (bases 1 to 3351)
  AUTHORS   Naar,A.M., Beaurang,P.A., Zhou,S., Abraham,S., Solomon,W. and
            Tjian,R.
  TITLE     Composite co-activator ARC mediates chromatin-directed
            transcriptional activation
  JOURNAL   Nature 398 (6730), 828-832 (1999)
   PUBMED   10235267
  REMARK    GeneRIF: The protein encoded by this gene was identified as ARC105,
            a 105kDa component of the Activator-Recruited Cofactor (ARC) that
            is involved in mediating gene activation by several classes of
            transcriptional regulators.
REFERENCE   10 (bases 1 to 3351)
  AUTHORS   Margolis,R.L., Abraham,M.R., Gatchell,S.B., Li,S.H., Kidwai,A.S.,
            Breschel,T.S., Stine,O.C., Callahan,C., McInnis,M.G. and Ross,C.A.
  TITLE     cDNAs with long CAG trinucleotide repeats from human brain
  JOURNAL   Hum Genet 100 (1), 114-122 (1997)
   PUBMED   9225980
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK090465.1, BC003078.1 and
            BC005027.1.
            
            On Nov 22, 2018 this sequence version replaced NM_001003891.2.
            
            Summary: The protein encoded by this gene is a subunit of the
            multiprotein complexes PC2 and ARC/DRIP and may function as a
            transcriptional coactivator in RNA polymerase II transcription.
            This gene contains stretches of trinucleotide repeats and is
            located in the chromosome 22 region which is deleted in DiGeorge
            syndrome. Alternative splicing results in multiple transcript
            variants. [provided by RefSeq, Jun 2014].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK090465.1, SRR11853563.21350.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000263205.11/ ENSP00000263205.7
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-530               AK090465.1         1-530
            531-1221            BC003078.1         520-1210
            1222-3349           AK090465.1         1222-3349
            3350-3351           BC005027.1         2719-2720
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q11.21"
     gene            1..3351
                     /gene="MED15"
                     /gene_synonym="ARC105; CAG7A; CTG7A; PCQAP; TIG-1; TIG1;
                     TNRC7"
                     /note="mediator complex subunit 15"
                     /db_xref="GeneID:51586"
                     /db_xref="HGNC:HGNC:14248"
                     /db_xref="MIM:607372"
     exon            1..137
                     /gene="MED15"
                     /gene_synonym="ARC105; CAG7A; CTG7A; PCQAP; TIG-1; TIG1;
                     TNRC7"
                     /inference="alignment:Splign:2.1.0"
     CDS             70..2436
                     /gene="MED15"
                     /gene_synonym="ARC105; CAG7A; CTG7A; PCQAP; TIG-1; TIG1;
                     TNRC7"
                     /note="isoform a is encoded by transcript variant 1;
                     PC2-glutamine-rich-associated protein; trinucleotide
                     repeat containing 7; TPA inducible gene-1; PC2 (positive
                     cofactor 2, multiprotein complex)
                     glutamine/Q-rich-associated protein; TPA inducible
                     protein; activator-recruited cofactor, 105-kD; CTG repeat
                     protein 7a; mediator of RNA polymerase II transcription
                     subunit 15; positive cofactor 2,
                     glutamine/Q-rich-associated protein; TPA-inducible gene 1
                     protein; PC2 glutamine/Q-rich-associated protein;
                     activator-recruited cofactor 105 kDa component;
                     trinucleotide repeat-containing gene 7 protein"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 15 isoform a"
                     /protein_id="NP_001003891.1"
                     /db_xref="CCDS:CCDS33602.1"
                     /db_xref="GeneID:51586"
                     /db_xref="HGNC:HGNC:14248"
                     /db_xref="MIM:607372"
                     /translation="MDVSGQETDWRSTAFRQKLVSQIEDAMRKAGVAHSKSSKDMESH
                     VFLKAKTRDEYLSLVARLIIHFRDIHNKKSQASVSDPMNALQSLTGGPAAGAAGIGMP
                     PRGPGQSLGGMGSLGAMGQPMSLSGQPPPGTSGMAPHSMAVVSTATPQTQLQLQQVAL
                     QQQQQQQQFQQQQQAALQQQQQQQQQQQFQAQQSAMQQQFQAVVQQQQQLQQQQQQQQ
                     HLIKLHHQNQQQIQQQQQQLQRIAQLQLQQQQQQQQQQQQQQQQALQAQPPIQQPPMQ
                     QPQPPPSQALPQQLQQMHHTQHHQPPPQPQQPPVAQNQPSQLPPQSQTQPLVSQAQAL
                     PGQMLYTQPPLKFVRAPMVVQQPPVQPQVQQQQTAVQTAQAAQMVAPGVQMITEALAQ
                     GGMHIRARFPPTTAVSAIPSSSIPLGRQPMAQVSQSSLPMLSSPSPGQQVQTPQSMPP
                     PPQPSPQPGQPSSQPNSNVSSGPAPSPSSFLPSPSPQPSQSPVTARTPQNFSVPSPGP
                     LNTPVNPSSVMSPAGSSQAEEQQYLDKLKQLSKYIEPLRRMINKIDKNEDRKKDLSKM
                     KSLLDILTDPSKRCPLKTLQKCEIALEKLKNDMAVPTPPPPPVPPTKQQYLCQPLLDA
                     VLANIRSPVFNHSLYRTFVPAMTAIHGPPITAPVVCTRKRRLEDDERQSIPSVLQGEV
                     ARLDPKFLVNLDPSHCSNNGTVHLICKLDDKDLPSVPPLELSVPADYPAQSPLWIDRQ
                     WQYDANPFLQSVHRCMTSRLLQLPDKHSVTALLNTWAQSVHQACLSAA"
     exon            138..225
                     /gene="MED15"
                     /gene_synonym="ARC105; CAG7A; CTG7A; PCQAP; TIG-1; TIG1;
                     TNRC7"
                     /inference="alignment:Splign:2.1.0"
     exon            226..277
                     /gene="MED15"
                     /gene_synonym="ARC105; CAG7A; CTG7A; PCQAP; TIG-1; TIG1;
                     TNRC7"
                     /inference="alignment:Splign:2.1.0"
     exon            278..307
                     /gene="MED15"
                     /gene_synonym="ARC105; CAG7A; CTG7A; PCQAP; TIG-1; TIG1;
                     TNRC7"
                     /inference="alignment:Splign:2.1.0"
     exon            308..520
                     /gene="MED15"
                     /gene_synonym="ARC105; CAG7A; CTG7A; PCQAP; TIG-1; TIG1;
                     TNRC7"
                     /inference="alignment:Splign:2.1.0"
     exon            521..759
                     /gene="MED15"
                     /gene_synonym="ARC105; CAG7A; CTG7A; PCQAP; TIG-1; TIG1;
                     TNRC7"
                     /inference="alignment:Splign:2.1.0"
     exon            760..1110
                     /gene="MED15"
                     /gene_synonym="ARC105; CAG7A; CTG7A; PCQAP; TIG-1; TIG1;
                     TNRC7"
                     /inference="alignment:Splign:2.1.0"
     exon            1111..1221
                     /gene="MED15"
                     /gene_synonym="ARC105; CAG7A; CTG7A; PCQAP; TIG-1; TIG1;
                     TNRC7"
                     /inference="alignment:Splign:2.1.0"
     exon            1222..1341
                     /gene="MED15"
                     /gene_synonym="ARC105; CAG7A; CTG7A; PCQAP; TIG-1; TIG1;
                     TNRC7"
                     /inference="alignment:Splign:2.1.0"
     exon            1342..1478
                     /gene="MED15"
                     /gene_synonym="ARC105; CAG7A; CTG7A; PCQAP; TIG-1; TIG1;
                     TNRC7"
                     /inference="alignment:Splign:2.1.0"
     exon            1479..1606
                     /gene="MED15"
                     /gene_synonym="ARC105; CAG7A; CTG7A; PCQAP; TIG-1; TIG1;
                     TNRC7"
                     /inference="alignment:Splign:2.1.0"
     exon            1607..1741
                     /gene="MED15"
                     /gene_synonym="ARC105; CAG7A; CTG7A; PCQAP; TIG-1; TIG1;
                     TNRC7"
                     /inference="alignment:Splign:2.1.0"
     exon            1742..1805
                     /gene="MED15"
                     /gene_synonym="ARC105; CAG7A; CTG7A; PCQAP; TIG-1; TIG1;
                     TNRC7"
                     /inference="alignment:Splign:2.1.0"
     exon            1806..1872
                     /gene="MED15"
                     /gene_synonym="ARC105; CAG7A; CTG7A; PCQAP; TIG-1; TIG1;
                     TNRC7"
                     /inference="alignment:Splign:2.1.0"
     exon            1873..2033
                     /gene="MED15"
                     /gene_synonym="ARC105; CAG7A; CTG7A; PCQAP; TIG-1; TIG1;
                     TNRC7"
                     /inference="alignment:Splign:2.1.0"
     exon            2034..2200
                     /gene="MED15"
                     /gene_synonym="ARC105; CAG7A; CTG7A; PCQAP; TIG-1; TIG1;
                     TNRC7"
                     /inference="alignment:Splign:2.1.0"
     exon            2201..2299
                     /gene="MED15"
                     /gene_synonym="ARC105; CAG7A; CTG7A; PCQAP; TIG-1; TIG1;
                     TNRC7"
                     /inference="alignment:Splign:2.1.0"
     exon            2300..3351
                     /gene="MED15"
                     /gene_synonym="ARC105; CAG7A; CTG7A; PCQAP; TIG-1; TIG1;
                     TNRC7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3329..3334
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED15"
                     /gene_synonym="ARC105; CAG7A; CTG7A; PCQAP; TIG-1; TIG1;
                     TNRC7"
                     /note="hexamer: AATAAA"
     polyA_site      3351
                     /gene="MED15"
                     /gene_synonym="ARC105; CAG7A; CTG7A; PCQAP; TIG-1; TIG1;
                     TNRC7"
                     /note="major polyA site"
ORIGIN      
        1 ggctctgtga ctgaggcggc ggcggtggcg gccaagcggg atacgggcgg cgggagctgg
       61 ggaacaggca tggacgtttc cgggcaagag accgactggc ggagcaccgc cttccggcag
      121 aagctggtca gtcaaatcga ggatgccatg aggaaagctg gtgtggcaca cagtaaatcc
      181 agcaaggata tggagagcca tgttttcctg aaggccaaga cccgggacga atacctttct
      241 ctcgtggcca ggctcattat ccattttcga gacattcata acaagaaatc tcaagcttcc
      301 gtcagtgatc ctatgaatgc actccagagc ctgactggcg gacctgctgc gggagccgct
      361 ggaattggca tgcctcctcg gggcccggga cagtctctgg gcgggatggg tagccttggt
      421 gccatgggac agccaatgtc tctctcaggg cagccgcctc ctgggacctc ggggatggcc
      481 cctcacagca tggctgtcgt gtctacggca actccacaga cccagctgca gctccagcag
      541 gtggcgctgc agcagcagca gcaacagcag cagttccagc agcagcagca ggcggcgcta
      601 cagcagcagc agcagcagca gcaacagcag cagttccagg ctcagcagag tgccatgcag
      661 cagcagttcc aagcagtagt gcagcagcag cagcagctcc agcagcagca gcagcagcag
      721 cagcatctaa ttaaattgca tcatcaaaat cagcaacaga tacagcagca gcaacagcag
      781 ctgcagcgaa tagcacagct gcagctccaa caacagcaac agcagcagca gcagcagcag
      841 cagcagcagc agcaggcttt gcaggcccag ccaccaattc agcagccacc gatgcagcag
      901 ccacagcctc cgccctccca ggctctgccc cagcagctgc agcagatgca tcacacacag
      961 caccaccagc cgccaccaca gccccagcag cctccagttg ctcagaacca accatcacaa
     1021 ctcccgccac agtcgcagac ccagcctttg gtgtcacagg cgcaagctct ccctggacaa
     1081 atgttgtata cccaaccacc actgaaattt gtccgagctc cgatggtggt gcagcagccc
     1141 ccagtgcagc cccaggtgca gcagcagcag acagcagtac agacagctca ggctgcccag
     1201 atggtggctc ccggagtcca gatgatcacg gaagccttgg cccaaggtgg gatgcacata
     1261 agagcccggt tcccgcctac caccgctgtg tccgccatcc cgtcaagctc catccctttg
     1321 ggcagacagc ccatggcaca ggtcagccag agcagcctcc ccatgctgtc ctcgccgtca
     1381 ccgggccagc aggtgcagac cccgcagtcg atgccccctc ccccccagcc gtccccgcag
     1441 cccggccagc ccagctcaca gcccaactcc aacgtcagct ctggccctgc cccatctccc
     1501 agtagcttcc tgcccagccc ctcaccgcag ccctcccaga gcccagtgac ggcgcggacc
     1561 ccacagaact tcagtgtccc ctcacctgga cctttaaaca cacctgtgaa ccccagctct
     1621 gtcatgagcc cagctggctc cagccaggct gaggagcagc agtacctgga caagctgaag
     1681 cagctgtcga agtacatcga gcccctgcgc cgcatgatca acaagatcga caagaacgaa
     1741 gacagaaaaa aggacctgag taagatgaag agccttctgg acattctgac agacccctcg
     1801 aagcggtgtc ccctgaagac cttgcaaaag tgtgagatcg ccctggagaa actcaagaat
     1861 gacatggcgg tgcccactcc cccaccgccc ccggtgccac cgaccaaaca gcagtaccta
     1921 tgccagccgc tcctggatgc cgtcctggcc aacatccgct cacctgtctt caaccattcc
     1981 ctgtaccgca cattcgttcc agccatgacc gccattcacg gcccacccat cacggcccca
     2041 gtggtgtgca cccggaagcg caggcttgag gatgatgagc ggcagagcat ccccagtgtg
     2101 ctccagggtg aggtggccag gctggacccc aagttcctgg taaacctgga cccttctcac
     2161 tgcagcaaca atggcactgt ccacctgatc tgcaagctgg atgacaagga cctcccaagt
     2221 gtgccaccac tggagctcag tgtgcccgct gactatcctg cccaaagccc gctgtggata
     2281 gaccggcagt ggcagtacga cgccaacccc ttcctccagt cggtgcaccg ctgcatgacc
     2341 tccaggctgc tgcagctccc ggacaagcac tcggtcaccg ccttgctcaa cacctgggcc
     2401 cagagcgtcc accaggcctg cctctcagcc gcctagccaa gactgcaggg atggcccgca
     2461 gcctcatcgg ggccaaggac acacgcctcc tgtcagacac ttctaggtgt tggcttcctt
     2521 agagagcctg gggttaggtt agctttcctg cttttatctt ctgccttggg gacctgccaa
     2581 acgaaatccc acacctgtac agaactggga taggcgcagt ggagcgggtt gcttgggggg
     2641 cgttggccga cttcttagag aaggccctcc atgtgacttc ctcccaggag ccagatgcga
     2701 tcctcaggct gctctcaccg tggcctgtcc acggtccagg tccatctcag cagcgtgagg
     2761 gtgcactcag ggtgttgtta gagcgtctcg tgtgtgctag acgcacccct actcgttcct
     2821 atagaacaca gaggacatag gaaaccctta aaacacacat gggattctct ggtcacagtt
     2881 ttgggttcag gctatgctgc tttgggcagg tggagcaccc cccgaggaag cctgcaagtc
     2941 cagggcacag gctgcctttt ggagggaggg ctggcccata ggtgctgctg gctccccgcc
     3001 accagctggg cctcagccct cacggcattc ctgctgagca ccgtggggca cccagggagc
     3061 aggggcgtca gggatcctgc tgccggcacc cctgtgccgc tggcatgagg gccgtgtccc
     3121 cactgtgaag gatgaagagc aaggccctca ggacccgtgt cctcagagca ccacacactg
     3181 agcacccaga gacagcgggc ctggcagcgg gccgggccat gcagggagcg cctccctatg
     3241 ttgcctgcca ctctgggcac cggccagcac cctctggtga gaagaggtcc ccccttttta
     3301 tgtgcactac cccaccatct gtgattataa taaatttatt attcctgtgt t
//
