LOCUS       NM_005121              10461 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens mediator complex subunit 13 (MED13), mRNA.
ACCESSION   NM_005121
VERSION     NM_005121.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10461)
  AUTHORS   Ren,M., Yang,Y., Heng,K.H.Y., Ng,L.Y., Chong,C.Y., Ng,Y.T.,
            Gorur-Shandilya,S., Lee,R.M.Q., Lim,K.L., Zhang,J. and Koh,T.W.
  TITLE     MED13 and glycolysis are conserved modifiers of
            alpha-synuclein-associated neurodegeneration
  JOURNAL   Cell Rep 41 (12), 111852 (2022)
   PUBMED   36543134
  REMARK    GeneRIF: MED13 and glycolysis are conserved modifiers of
            alpha-synuclein-associated neurodegeneration.
REFERENCE   2  (bases 1 to 10461)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 10461)
  AUTHORS   De Nardi,L., Faletra,F., D'Adamo,A.P., Bianco,A.M.R.,
            Athanasakis,E., Bruno,I. and Barbi,E.
  TITLE     Could the MED13 mutations manifest as a Kabuki-like syndrome?
  JOURNAL   Am J Med Genet A 185 (2), 584-590 (2021)
   PUBMED   33258286
  REMARK    GeneRIF: Could the MED13 mutations manifest as a Kabuki-like
            syndrome?
REFERENCE   4  (bases 1 to 10461)
  AUTHORS   Zhou,W., Cai,H., Li,J., Xu,H., Wang,X., Men,H., Zheng,Y. and Cai,L.
  TITLE     Potential roles of mediator Complex Subunit 13 in Cardiac Diseases
  JOURNAL   Int J Biol Sci 17 (1), 328-338 (2021)
   PUBMED   33390853
  REMARK    GeneRIF: Potential roles of mediator Complex Subunit 13 in Cardiac
            Diseases.
            Review article
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 10461)
  AUTHORS   Snijders Blok,L., Hiatt,S.M., Bowling,K.M., Prokop,J.W.,
            Engel,K.L., Cochran,J.N., Bebin,E.M., Bijlsma,E.K.,
            Ruivenkamp,C.A.L., Terhal,P., Simon,M.E.H., Smith,R., Hurst,J.A.,
            McLaughlin,H., Person,R., Crunk,A., Wangler,M.F., Streff,H.,
            Symonds,J.D., Zuberi,S.M., Elliott,K.S., Sanders,V.R., Masunga,A.,
            Hopkin,R.J., Dubbs,H.A., Ortiz-Gonzalez,X.R., Pfundt,R.,
            Brunner,H.G., Fisher,S.E., Kleefstra,T. and Cooper,G.M.
  CONSRTM   DDD study
  TITLE     De novo mutations in MED13, a component of the Mediator complex,
            are associated with a novel neurodevelopmental disorder
  JOURNAL   Hum Genet 137 (5), 375-388 (2018)
   PUBMED   29740699
  REMARK    GeneRIF: De novo mutations in MED13, a component of the Mediator
            complex, are associated with a novel neurodevelopmental disorder.
REFERENCE   6  (bases 1 to 10461)
  AUTHORS   Naar,A.M., Beaurang,P.A., Zhou,S., Abraham,S., Solomon,W. and
            Tjian,R.
  TITLE     Composite co-activator ARC mediates chromatin-directed
            transcriptional activation
  JOURNAL   Nature 398 (6730), 828-832 (1999)
   PUBMED   10235267
REFERENCE   7  (bases 1 to 10461)
  AUTHORS   Rachez,C., Lemon,B.D., Suldan,Z., Bromleigh,V., Gamble,M.,
            Naar,A.M., Erdjument-Bromage,H., Tempst,P. and Freedman,L.P.
  TITLE     Ligand-dependent transcription activation by nuclear receptors
            requires the DRIP complex
  JOURNAL   Nature 398 (6730), 824-828 (1999)
   PUBMED   10235266
REFERENCE   8  (bases 1 to 10461)
  AUTHORS   Ito,M., Yuan,C.X., Malik,S., Gu,W., Fondell,J.D., Yamamura,S.,
            Fu,Z.Y., Zhang,X., Qin,J. and Roeder,R.G.
  TITLE     Identity between TRAP and SMCC complexes indicates novel pathways
            for the function of nuclear receptors and diverse mammalian
            activators
  JOURNAL   Mol Cell 3 (3), 361-370 (1999)
   PUBMED   10198638
REFERENCE   9  (bases 1 to 10461)
  AUTHORS   Gu,W., Malik,S., Ito,M., Yuan,C.X., Fondell,J.D., Zhang,X.,
            Martinez,E., Qin,J. and Roeder,R.G.
  TITLE     A novel human SRB/MED-containing cofactor complex, SMCC, involved
            in transcription regulation
  JOURNAL   Mol Cell 3 (1), 97-108 (1999)
   PUBMED   10024883
  REMARK    Erratum:[Mol Cell 1999 Apr;3(4):following 541]
REFERENCE   10 (bases 1 to 10461)
  AUTHORS   Fondell,J.D., Ge,H. and Roeder,R.G.
  TITLE     Ligand induction of a transcriptionally active thyroid hormone
            receptor coactivator complex
  JOURNAL   Proc Natl Acad Sci U S A 93 (16), 8329-8333 (1996)
   PUBMED   8710870
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP332524.1, BQ709424.1,
            AF117754.1, AB011165.2, AC060798.10 and CN273870.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_005121.2.
            
            Summary: This gene encodes a component of the mediator complex
            (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional
            coactivator complex thought to be required for the expression of
            almost all genes. The mediator complex is recruited by
            transcriptional activators or nuclear receptors to induce gene
            expression, possibly by interacting with RNA polymerase II and
            promoting the formation of a transcriptional pre-initiation
            complex. The product of this gene is proposed to form a sub-complex
            with MED12, cyclin C, and CDK8 that can negatively regulate
            transactivation by mediator. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF117754.1, BC140891.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000397786.7/ ENSP00000380888.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-429               BP332524.1         5-433
            430-433             BQ709424.1         190-193
            434-1247            AF117754.1         438-1251
            1248-4392           AB011165.2         670-3814
            4393-4393           AC060798.10        152133-152133       c
            4394-8327           AB011165.2         3816-7749
            8328-10175          AC060798.10        128501-130348       c
            10176-10461         CN273870.1         1-286
FEATURES             Location/Qualifiers
     source          1..10461
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q23.2"
     gene            1..10461
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /note="mediator complex subunit 13"
                     /db_xref="GeneID:9969"
                     /db_xref="HGNC:HGNC:22474"
                     /db_xref="MIM:603808"
     exon            1..139
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /inference="alignment:Splign:2.1.0"
     CDS             74..6598
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /note="thyroid hormone receptor-associated protein, 240
                     kDa subunit; thyroid hormone receptor-associated protein
                     complex component TRAP240; vitamin D3 receptor-interacting
                     protein complex component DRIP250; activator-recruited
                     cofactor 250 kDa component; mediator of RNA polymerase II
                     transcription, subunit 13 homolog; thyroid hormone
                     receptor-associated protein 1; thyroid hormone
                     receptor-associated protein complex 240 kDa component"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 13"
                     /protein_id="NP_005112.2"
                     /db_xref="CCDS:CCDS42366.1"
                     /db_xref="GeneID:9969"
                     /db_xref="HGNC:HGNC:22474"
                     /db_xref="MIM:603808"
                     /translation="MSASFVPNGASLEDCHCNLFCLADLTGIKWKKYVWQGPTSAPIL
                     FPVTEEDPILSSFSRCLKADVLGVWRRDQRPGRRELWIFWWGEDPSFADLIHHDLSEE
                     EDGVWENGLSYECRTLLFKAVHNLLERCLMNRNFVRIGKWFVKPYEKDEKPINKSEHL
                     SCSFTFFLHGDSNVCTSVEINQHQPVYLLSEEHITLAQQSNSPFQVILCPFGLNGTLT
                     GQAFKMSDSATKKLIGEWKQFYPISCCLKEMSEEKQEDMDWEDDSLAAVEVLVAGVRM
                     IYPACFVLVPQSDIPTPSPVGSTHCSSSCLGVHQVPASTRDPAMSSVTLTPPTSPEEV
                     QTVDPQSVQKWVKFSSVSDGFNSDSTSHHGGKIPRKLANHVVDRVWQECNMNRAQNKR
                     KYSASSGGLCEEATAAKVASWDFVEATQRTNCSCLRHKNLKSRNAGQQGQAPSLGQQQ
                     QILPKHKTNEKQEKSEKPQKRPLTPFHHRVSVSDDVGMDADSASQRLVISAPDSQVRF
                     SNIRTNDVAKTPQMHGTEMANSPQPPPLSPHPCDVVDEGVTKTPSTPQSQHFYQMPTP
                     DPLVPSKPMEDRIDSLSQSFPPQYQEAVEPTVYVGTAVNLEEDEANIAWKYYKFPKKK
                     DVEFLPPQLPSDKFKDDPVGPFGQESVTSVTELMVQCKKPLKVSDELVQQYQIKNQCL
                     SAIASDAEQEPKIDPYAFVEGDEEFLFPDKKDRQNSEREAGKKHKVEDGTSSVTVLSH
                     EEDAMSLFSPSIKQDAPRPTSHARPPSTSLIYDSDLAVSYTDLDNLFNSDEDELTPGS
                     KKSANGSDDKASCKESKTGNLDPLSCISTADLHKMYPTPPSLEQHIMGFSPMNMNNKE
                     YGSMDTTPGGTVLEGNSSSIGAQFKIEVDEGFCSPKPSEIKDFSYVYKPENCQILVGC
                     SMFAPLKTLPSQYLPPIKLPEECIYRQSWTVGKLELLSSGPSMPFIKEGDGSNMDQEY
                     GTAYTPQTHTSFGMPPSSAPPSNSGAGILPSPSTPRFPTPRTPRTPRTPRGAGGPASA
                     QGSVKYENSDLYSPASTPSTCRPLNSVEPATVPSIPEAHSLYVNLILSESVMNLFKDC
                     NFDSCCICVCNMNIKGADVGVYIPDPTQEAQYRCTCGFSAVMNRKFGNNSGLFLEDEL
                     DIIGRNTDCGKEAEKRFEALRATSAEHVNGGLKESEKLSDDLILLLQDQCTNLFSPFG
                     AADQDPFPKSGVISNWVRVEERDCCNDCYLALEHGRQFMDNMSGGKVDEALVKSSCLH
                     PWSKRNDVSMQCSQDILRMLLSLQPVLQDAIQKKRTVRPWGVQGPLTWQQFHKMAGRG
                     SYGTDESPEPLPIPTFLLGYDYDYLVLSPFALPYWERLMLEPYGSQRDIAYVVLCPEN
                     EALLNGAKSFFRDLTAIYESCRLGQHRPVSRLLTDGIMRVGSTASKKLSEKLVAEWFS
                     QAADGNNEAFSKLKLYAQVCRYDLGPYLASLPLDSSLLSQPNLVAPTSQSLITPPQMT
                     NTGNANTPSATLASAASSTMTVTSGVAISTSVATANSTLTTASTSSSSSSNLNSGVSS
                     NKLPSFPPFGSMNSNAAGSMSTQANTVQSGQLGGQQTSALQTAGISGESSSLPTQPHP
                     DVSESTMDRDKVGIPTDGDSHAVTYPPAIVVYIIDPFTYENTDESTNSSSVWTLGLLR
                     CFLEMVQTLPPHIKSTVSVQIIPCQYLLQPVKHEDREIYPQHLKSLAFSAFTQCRRPL
                     PTSTNVKTLTGFGPGLAMETALRSPDRPECIRLYAPPFILAPVKDKQTELGETFGEAG
                     QKYNVLFVGYCLSHDQRWILASCTDLYGELLETCIINIDVPNRARRKKSSARKFGLQK
                     LWEWCLGLVQMSSLPWRVVIGRLGRIGHGELKDWSCLLSRRNLQSLSKRLKDMCRMCG
                     ISAADSPSILSACLVAMEPQGSFVIMPDSVSTGSVFGRSTTLNMQTSQLNTPQDTSCT
                     HILVFPTSASVQVASATYTTENLDLAFNPNNDGADGMGIFDLLDTGDDLDPDIINILP
                     ASPTGSPVHSPGSHYPHGGDAGKGQSTDRLLSTEPHEEVPNILQQPLALGYFVSTAKA
                     GPLPDWFWSACPQAQYQCPLFLKASLHLHVPSVQSDELLHSKHSHPLDSNQTSDVLRF
                     VLEQYNALSWLTCDPATQDRRSCLPIHFVVLNQLYNFIMNML"
     exon            140..374
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /inference="alignment:Splign:2.1.0"
     exon            375..543
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /inference="alignment:Splign:2.1.0"
     exon            544..689
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /inference="alignment:Splign:2.1.0"
     exon            690..887
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /inference="alignment:Splign:2.1.0"
     exon            888..1082
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /inference="alignment:Splign:2.1.0"
     exon            1083..1245
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /inference="alignment:Splign:2.1.0"
     exon            1246..1356
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /inference="alignment:Splign:2.1.0"
     exon            1357..2040
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /inference="alignment:Splign:2.1.0"
     exon            2041..2254
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /inference="alignment:Splign:2.1.0"
     exon            2255..2336
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /inference="alignment:Splign:2.1.0"
     exon            2337..2458
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /inference="alignment:Splign:2.1.0"
     exon            2459..2549
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /inference="alignment:Splign:2.1.0"
     exon            2550..2764
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /inference="alignment:Splign:2.1.0"
     exon            2765..2961
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /inference="alignment:Splign:2.1.0"
     exon            2962..3878
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /inference="alignment:Splign:2.1.0"
     exon            3879..4040
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /inference="alignment:Splign:2.1.0"
     exon            4041..4264
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /inference="alignment:Splign:2.1.0"
     exon            4265..4454
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /inference="alignment:Splign:2.1.0"
     exon            4455..4917
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /inference="alignment:Splign:2.1.0"
     exon            4918..5137
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /inference="alignment:Splign:2.1.0"
     exon            5138..5329
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /inference="alignment:Splign:2.1.0"
     exon            5330..5553
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /inference="alignment:Splign:2.1.0"
     exon            5554..5696
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /inference="alignment:Splign:2.1.0"
     exon            5697..5855
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /inference="alignment:Splign:2.1.0"
     exon            5856..6041
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /inference="alignment:Splign:2.1.0"
     exon            6042..6190
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /inference="alignment:Splign:2.1.0"
     exon            6191..6364
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /inference="alignment:Splign:2.1.0"
     exon            6365..6465
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /inference="alignment:Splign:2.1.0"
     exon            6466..10461
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9736..9741
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /note="hexamer: ATTAAA"
     polyA_site      9757
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
     regulatory      10438..10443
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /note="hexamer: AATAAA"
     polyA_site      10461
                     /gene="MED13"
                     /gene_synonym="ARC250; DRIP250; HSPC221; MRD61; THRAP1;
                     TRAP240"
                     /note="major polyA site"
ORIGIN      
        1 ctctctctgg tcggaggcgg cggtaatggc ggatggtggg ttgtggcgcc ggcggcggct
       61 gctgtgaggg acgatgagtg cctccttcgt gccgaacggg gccagcctgg aagattgtca
      121 ctgtaacctc ttctgcctgg ctgacttgac aggaattaag tggaaaaaat atgtatggca
      181 aggcccaact tctgccccta ttctgtttcc tgtgacagaa gaagacccca ttttgagcag
      241 ttttagtcgc tgccttaagg cagatgtact tggtgtttgg cggcgagatc aaagacctgg
      301 aagaagagaa ttgtggatat tttggtgggg tgaagacccc agttttgctg accttattca
      361 ccatgactta tcagaagaag aagatggagt gtgggagaat ggactttcct atgaatgccg
      421 tactctgctt ttcaaagcag ttcacaatct attggaacgg tgtttaatga acaggaattt
      481 tgtacgtatt ggcaagtggt ttgtaaagcc ttatgaaaaa gatgaaaaac ctataaataa
      541 aagtgaacac ttgtcctgct ccttcacctt tttcttgcat ggagacagca atgtttgtac
      601 cagtgtggaa attaaccaac atcaacctgt ataccttctc agtgaagagc atatcaccct
      661 tgctcaacag tctaatagcc catttcaagt tatcttatgc ccatttggac taaatggcac
      721 tctcacagga caggcattca agatgtctga ttcagctaca aaaaaattaa ttggtgaatg
      781 gaaacagttc tatcctatct catgttgctt gaaggagatg tctgaagaaa aacaggaaga
      841 tatggattgg gaagatgatt ctttagctgc agtagaagtt cttgttgctg gtgtccgaat
      901 gatctaccca gcatgctttg ttctagtccc tcagtcagac attcctactc ctagccctgt
      961 gggatccact cactgttcat cttcttgctt gggtgtccac caagtgcctg cttccacaag
     1021 agatcctgct atgtcttcgg ttacgcttac accacctacg tctcctgagg aagtccaaac
     1081 agttgatcct cagtctgtcc agaagtgggt caaattttct tcagtatctg atggcttcaa
     1141 ctccgatagt actagccacc atggtgggaa aatacccaga aaattagcaa atcatgtggt
     1201 ggatagagtt tggcaagaat gcaatatgaa cagagcacag aacaagagga agtattctgc
     1261 ttcatcaggt ggtctatgcg aagaagcgac agctgctaaa gtggcatcct gggattttgt
     1321 tgaagccaca caaagaacaa attgcagttg tttgaggcac aaaaatctca agtcaagaaa
     1381 tgctggacaa caaggacagg caccatcttt aggtcagcaa caacaaatac ttcctaagca
     1441 caagaccaat gagaagcaag aaaagagtga aaagccacag aaacgcccct tgactccttt
     1501 tcaccatcgt gtgtctgtta gtgatgatgt tggcatggac gcagattcag ccagccaaag
     1561 acttgtgatc tctgctccag acagtcaagt gagattttca aatatccgaa ctaatgatgt
     1621 agcaaagact cctcagatgc atggcaccga aatggcaaat tcacctcaac cacccccact
     1681 tagtcctcac ccttgtgatg tggttgatga aggagtgact aaaacacctt caactcctca
     1741 gagtcaacat ttttatcaaa tgccaacacc agatcccttg gttccttcta aaccaatgga
     1801 agataggata gacagtttgt cccagtcttt cccacctcaa tatcaggaag ctgtagaacc
     1861 tacagtatat gttggtacag cagtaaactt ggaagaagat gaagccaata tagcctggaa
     1921 gtattacaag ttcccaaaga aaaaagatgt agagttttta ccacctcaac ttccaagtga
     1981 taaattcaag gatgatccag ttggaccttt tggacaggaa agtgtaacat cagttacaga
     2041 gttaatggtg caatgtaaga aacctttaaa agtttctgat gaattagtgc agcaatatca
     2101 aattaaaaac cagtgtcttt cagcaatagc atctgatgca gaacaagaac ctaaaattga
     2161 tccatatgca tttgttgaag gagatgagga attccttttt cctgataaaa aagatagaca
     2221 aaatagtgag agagaagctg gaaaaaaaca caaggtagaa gatgggacat ctagtgtaac
     2281 agtgttatca catgaagaag atgctatgtc attatttagt ccctctatca agcaagatgc
     2341 tccacgccct actagtcatg cccgtcctcc atcaacaagt ttgatttatg actcagacct
     2401 ggctgtctct tatactgacc ttgataatct cttcaattct gatgaagatg aactaacacc
     2461 tggatctaaa aaatcagcaa atggatcaga tgataaagcc agctgcaagg aatcaaagac
     2521 aggaaatctg gacccgttat cttgcataag cactgcagat cttcataaaa tgtatcctac
     2581 accaccatca ttggaacaac atattatggg attttcccca atgaatatga ataataaaga
     2641 atatggtagt atggatacaa cacctggagg aactgttcta gaaggaaata gttctagtat
     2701 aggagcgcag ttcaaaattg aggttgatga gggattctgt agccccaaac cttctgaaat
     2761 taaagatttt tcttatgtct ataagcctga aaattgtcaa attctagtgg gatgttccat
     2821 gtttgcacct ctaaaaactc taccaagcca atatctgccc cctatcaaat tgccagaaga
     2881 gtgtatttac cgtcagagtt ggactgttgg aaaattggaa ttgctttctt cagggccttc
     2941 aatgccattc atcaaagagg gtgatggaag taatatggat caagaatatg gcactgctta
     3001 tacacctcaa actcatactt cttttgggat gcctcctagc agtgcacctc ctagtaacag
     3061 cggagcagga attcttcctt ctccatccac ccctcggttt ccaactccaa ggactccaag
     3121 gactcctcgg actcctcgtg gagctggtgg acctgctagt gctcaaggtt cagtcaaata
     3181 tgaaaattca gacttgtatt caccagcttc taccccatct acatgcagac cccttaattc
     3241 tgttgaacct gcaactgtcc cttccatccc tgaagcacac agtctttatg taaacctcat
     3301 cctttcagaa tcagttatga atttgtttaa agactgtaac tttgatagtt gttgcatctg
     3361 tgtttgcaac atgaacatca agggtgccga tgttggagtt tacattccag atccaacgca
     3421 ggaagcacaa tataggtgta cctgtggctt cagtgctgtc atgaacagaa aatttggaaa
     3481 caattcagga ttatttcttg aagatgaact agatatcata ggacgcaata cagactgtgg
     3541 caaagaagca gaaaaacgtt ttgaagctct cagggctacc tctgctgaac atgttaatgg
     3601 aggactaaag gaatctgaaa aattatctga tgatttgata ttattgctac aagatcagtg
     3661 cactaattta ttttcaccct ttggagcagc agaccaagat ccttttccta aaagtggtgt
     3721 aattagcaat tgggtacgtg ttgaagagcg tgactgttgc aatgactgct accttgcatt
     3781 agaacatggg cgtcagttca tggataacat gtcaggagga aaagttgatg aagcacttgt
     3841 gaaaagttca tgcttacacc cctggtccaa aagaaacgat gtgagtatgc agtgctcaca
     3901 ggatatactt cgaatgctcc tctctcttca gccagttctt caggatgcca ttcagaaaaa
     3961 aagaacagta agaccttggg gtgttcaggg tcctctcact tggcaacaat ttcataaaat
     4021 ggctggccga ggctcttatg gaactgatga atccccagaa ccactgccaa tccccacatt
     4081 tttgttgggt tatgattatg attatctggt gctttctcca tttgctcttc cttattggga
     4141 gagacttatg ctggaaccct atggatctca aagagatata gcctatgttg tactgtgtcc
     4201 agaaaatgaa gccttgttaa atggagcaaa aagctttttt agagatctta ctgcaatata
     4261 tgagtcctgt cgattaggtc aacatagacc tgtttctcga ctgttaacag atgggatcat
     4321 gagagttgga tctactgcat caaagaaact atcagaaaag ttggtagcag aatggttttc
     4381 tcaggcagct gacggtaaca atgaagcatt ttctaaactc aagctttatg cacaagtctg
     4441 cagatatgac ctaggtcctt atcttgcttc cctgccattg gacagctctc tactttccca
     4501 gccaaattta gttgccccta caagtcagtc tttgattact ccacctcaga tgacaaatac
     4561 tggaaatgct aatactccat ctgccacctt agcatctgca gcgagcagca ctatgacagt
     4621 gacttcaggt gttgccatat ctacttcagt tgccacagct aattcaactt tgaccacagc
     4681 ttcaacttca tcttcatcat cctccaactt gaatagtgga gtatcatcaa ataaactacc
     4741 ttcgtttcca ccctttggca gtatgaacag taatgctgca ggatccatgt ctacacaagc
     4801 aaatacagtt cagagtggtc agctaggagg gcaacagaca tcagctctac agacagctgg
     4861 gatttctgga gaatcatctt cacttcccac tcagccgcat cctgatgtgt ctgaaagcac
     4921 gatggatcgg gataaagtgg gaatccccac agatggtgat tcacatgcag tcacgtatcc
     4981 acctgcaatt gttgtttata taattgatcc ttttacatac gaaaatacag acgagagcac
     5041 taactcttct agtgtgtgga cattggggct acttcgatgc tttctagaaa tggtccagac
     5101 tcttcctcct catatcaaga gtactgtttc tgtacagatt attccttgtc agtacctgtt
     5161 gcaacctgtg aagcatgaag atagagaaat ctatccccag catttaaaat ccctggcttt
     5221 ttcggccttt acccagtgtc ggaggccact tccaacatca accaatgtga aaacattgac
     5281 tggctttggt ccaggtttag ccatggaaac tgcccttaga agtcctgata gaccagagtg
     5341 tattcgactt tatgcacctc cttttattct ggctccagtg aaggacaaac agacagagct
     5401 aggagaaaca tttggagaag ctggacagaa atataatgtt ctttttgtgg gatactgttt
     5461 atcacatgat caaaggtgga ttcttgcatc ttgcacagat ctatatggag aacttttaga
     5521 aacttgtatc attaacatcg atgttccaaa tagggctcgt cggaaaaaaa gttctgctag
     5581 aaaatttggt ctacagaaac tttgggagtg gtgcttagga cttgtacaaa tgagttcatt
     5641 gccatggaga gttgtaattg gtcgtctagg aaggattggt catggagaat tgaaagattg
     5701 gagctgtttg ctgagtcgtc gaaacttgca gtctctaagt aaaaggctca aagacatgtg
     5761 tagaatgtgt ggtatatctg ctgcagactc ccctagcatt ctcagtgctt gcttggtggc
     5821 aatggagccg caaggctctt ttgttattat gccagattct gtgtcaactg gttctgtatt
     5881 tggaagaagc acgactctaa atatgcagac atctcagcta aataccccac aggatacatc
     5941 atgtactcat atacttgtgt ttcctacttc tgcttctgtg caagtagctt cagctactta
     6001 taccactgaa aatttggatt tagctttcaa tcccaacaat gatggagcag atggaatggg
     6061 tatctttgat ttgttagaca caggagatga tcttgaccct gatatcatta atatccttcc
     6121 tgcttctcca actggttctc ctgtacattc tccaggatct cattaccccc atggaggtga
     6181 tgcgggcaag ggtcagagta ctgatcggct actatcaaca gaacctcatg aggaagtacc
     6241 taatattctt cagcaaccat tggcccttgg ttactttgta tcaactgcca aagcaggtcc
     6301 attacctgac tggttctggt cagcatgtcc tcaagcacaa tatcagtgtc ccctttttct
     6361 taaggcctct ttgcacctcc acgtgccttc agtgcaatct gacgagctgc ttcacagtaa
     6421 acactcccac ccacttgact caaatcagac ttcagatgtc ctcaggtttg ttttggaaca
     6481 gtacaatgca ctctcctggc taacctgtga ccctgcaacc caggacagac gctcatgtct
     6541 cccaattcat tttgtggtgc tgaatcagtt atataacttt attatgaata tgctgtgatc
     6601 ttcatttgat ggaactgtgc aagaaaagaa caaggaaaaa tggatgtttc gctgcaggat
     6661 taagttacaa ttatcttctc agtgaaggtc atttgtgatg gggtctaatt cttattactt
     6721 caacaaatat tgttttgact tggggggagg ggctataacc ctgctatttt tcattgactc
     6781 tattgaactc tttaggatga tgactgatca tacaaaacgt attataacat tttcgtagca
     6841 aaattaacct tttttttttc cagtcacagt atttgtgaaa agtaatgagc catagtaccc
     6901 agtcatgtta aatgaatatt aaaagcatgg agaggaaaca tgaggaacaa tgaatttcaa
     6961 catatggctt cagaacatga agatgttctt gtatggatta tagtatctag tattcaaaaa
     7021 tgcctgcatc tcttctctta tttattgtaa gtttttaaat gtataaattg tcttatattt
     7081 cttaacctct tttataaaaa ttttcctaga aggtttatac tgccttcttg ctttaaagca
     7141 attggtctaa aatatatgta atcgtcttaa ttaaaaagtt gcagtagggt tgcttttaga
     7201 gtattatttt tttgtaaggg ggtgggtggg acagtaaatt tgtattgtct cgatgtacag
     7261 tttaacgggg atagaggggg aataatgtcc ataccattgt gtgtggagga tttacagcta
     7321 agctgtagtt gcagagtaca tgtacagtaa tgaagttcac tgtgtttata aattgaaaag
     7381 gtaccaggtc ttacagcatt ttatatatca catctttaca gaataacatg atggcaatat
     7441 acaagtggta ttgttaggtg gtttaactta gaataaaatg agaattcttc agttatattt
     7501 tgtactatgg tttagggcta tgactaatat ttcaggccat ttccggtgaa agaaacttag
     7561 ttttacaaga aaaaccattt gctactgaat gcttaaacta attttagtgt ttaatgttac
     7621 atgcttaaat ttttttcagt tttaacagtg gcatatttag gcatggaaat attattatga
     7681 aatttatttt caggatctgc tataaggttg aaatttagcc cagctctagg cattttacaa
     7741 attatttttc aagcagtcat tcttgattgt ttgacttttt tttttaaatt aaagattggg
     7801 aatgtatgtg agagtatgca tatgtatggg tgtgtgtgtg tgcgcgcaat caaactgtgg
     7861 tgtaaataga ttctcagtga attctggtat tcagactcta ttccactagt gaaagaacca
     7921 ttttttaaac ttcccttgcc ttttttattt atttaatttt cttggtttgg agatgtcagt
     7981 cccaaacacc agagtctgta cttttctata acacagctca gattaaggta gggcatatgc
     8041 caaggaggtt ctcacctccc taaagaaggg acttgaattt tagggacttt aattcacccc
     8101 tccttcaata caactttccc ccttcttgtt tgcacatgcc aagataactg cttttatgca
     8161 ggctgtaccc ccttgaaaaa tcctttctac agtgctgctc acaaaagagc ccaagttcgc
     8221 ctcctacctg cattgctgac ttgaattcac agtcgccgag tctacctagc tttcttggaa
     8281 gcagtctagc aaaatttcta tttgtacgtt cactaattat ctacaaggac aaaatcagtt
     8341 gtatttacaa aactctactt cagtgtttgt tttagttttt ttttttactg aaacttgttt
     8401 ttgtgaatac tctgtgctta gaattaaata tcactttctt atgaacaaca taacttcttc
     8461 agattgtgta tatgaaaaca ttagcaagtc ttgttttttc tatgaagcaa acacaattgg
     8521 tgacaaaggt tgtcaatcat ttcttcaaaa ttataatgca gttctaatgg tcagcatatt
     8581 ttgatattaa atttaaagat cacctctctg catttgtttt taaattatgc taatacacca
     8641 cacattatgt tggtatgttt tgttctgtac tttctttaaa aaaaaaaaaa aaacttgtct
     8701 gagatttgaa ggaaaatgtg cttatttgga atttccataa aaagagtatc ctttttatac
     8761 acttaatagt gactttacaa aataaaagtt atattctcag ttgtttaaaa tcactaacct
     8821 atgataacca cacctcaatt tgaaagtaga tttaaaatta ttccctgaca ggttatttaa
     8881 tatggagcca taaggaggga acccagtaca caattatttt ttatttggga atcagggaat
     8941 agttcccaaa tatacaggat ttattgataa gatttttttt cttcccttca tatatccatt
     9001 caaactcaat ggaaagttat taaataacca ttagaaaagc tcagtagact tatttgagaa
     9061 attaagcctt gtgcaggatg atggatttga cttactaatg tactgtcaca gacaaatatg
     9121 ggtagttttg tttaaatagg taagcaaaat attatacttt atagcagtgg attaccaaca
     9181 ccttgacttc tttgttacag tgctaacatc tttttttttg tgcaggtatc catgattatt
     9241 aagcagggtg gaagttcagt attttgtcat ttaaaaagat tagttatata atgtctgctt
     9301 ccagccagtg agaaacatct agccatacct ttcttatgca agccattgag ttatcaggac
     9361 tgtgaattaa cactgtatga ataaatttct gtacacctta ttgtttggcc agaaggccac
     9421 caagtgtact tatatgtaat ccttaaattt taaagtagct gtaattttta aatatttcta
     9481 aacttttctt aaaccactaa aattaagctc ttactactta gtcaactatc ctcagctgta
     9541 ttcgtactca attgtcagta tggcacagat tactgtatta aaatattctc ctttcgtctt
     9601 catatttacc ttctgaggta attttttaac ttaatgtgtt actacaaaga tttgcagatc
     9661 tttaatcaag cactatgtta atactgtaat atcagaatac tatgttgcat tatttaaaat
     9721 gttcaaattg aatagattaa aaagttttta aatgctattg catcatataa tttgctattc
     9781 atccactatg gcattgcata tcaatcagtt aatacactta atgttgcatg agtgatattt
     9841 tggtctgggt ttcctcttaa gattttagtt tgtctgaatt aaggaaaaat gtttttaata
     9901 tacattctta ttttgtccca cccctccaga aataagctgg aaatcttaac tttttggggg
     9961 gtcttttttg gtgttttaat gggcccagaa ctgtggttta aatttttatg tatgtatttt
    10021 cttttttgtg gagtataaat ttaaaaactg gatttgggac ctaaaatact cctcaggttg
    10081 atgtattcat gaagttttaa aacatcttta gttttcaaag taaactggat atgtggacct
    10141 taaagttatt gagtttaagc tacaaattgt aacgtcatta ctggacatgt cagcatcaac
    10201 cctctcaaaa tagcttggtc actttatgaa ggggcgtttt aaagttgttg tttagcagtg
    10261 acatttaata tggtccaatt gcttttcttt ttaacgtgac aaaaagagaa taaggaacaa
    10321 acactattgc tgccgaatgc cataacactg agttgtacaa attgtgattg aggaaatgaa
    10381 aaggtttata ctttttaaaa aaaaaaaaac aaaaacaaaa aacaaaactt caaatggaat
    10441 aaattattca tgaagccttc a
//
