LOCUS       NM_032286               1101 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens mediator complex subunit 10 (MED10), mRNA.
ACCESSION   NM_032286
VERSION     NM_032286.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 1101)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 1101)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   5  (bases 1 to 1101)
  AUTHORS   Marcon,E., Ni,Z., Pu,S., Turinsky,A.L., Trimble,S.S., Olsen,J.B.,
            Silverman-Gavrila,R., Silverman-Gavrila,L., Phanse,S., Guo,H.,
            Zhong,G., Guo,X., Young,P., Bailey,S., Roudeva,D., Zhao,D.,
            Hewel,J., Li,J., Graslund,S., Paduch,M., Kossiakoff,A.A.,
            Lupien,M., Emili,A., Wodak,S.J. and Greenblatt,J.
  TITLE     Human-chromatin-related protein interactions identify a demethylase
            complex required for chromosome segregation
  JOURNAL   Cell Rep 8 (1), 297-310 (2014)
   PUBMED   24981860
REFERENCE   6  (bases 1 to 1101)
  AUTHORS   Petroziello,J., Yamane,A., Westendorf,L., Thompson,M., McDonagh,C.,
            Cerveny,C., Law,C.L., Wahl,A. and Carter,P.
  TITLE     Suppression subtractive hybridization and expression profiling
            identifies a unique set of genes overexpressed in non-small-cell
            lung cancer
  JOURNAL   Oncogene 23 (46), 7734-7745 (2004)
   PUBMED   15334068
REFERENCE   7  (bases 1 to 1101)
  AUTHORS   Sato,S., Tomomori-Sato,C., Parmely,T.J., Florens,L., Zybailov,B.,
            Swanson,S.K., Banks,C.A., Jin,J., Cai,Y., Washburn,M.P.,
            Conaway,J.W. and Conaway,R.C.
  TITLE     A set of consensus mammalian mediator subunits identified by
            multidimensional protein identification technology
  JOURNAL   Mol Cell 14 (5), 685-691 (2004)
   PUBMED   15175163
REFERENCE   8  (bases 1 to 1101)
  AUTHORS   Sato,S., Tomomori-Sato,C., Banks,C.A., Parmely,T.J., Sorokina,I.,
            Brower,C.S., Conaway,R.C. and Conaway,J.W.
  TITLE     A mammalian homolog of Drosophila melanogaster transcriptional
            coactivator intersex is a subunit of the mammalian Mediator complex
  JOURNAL   J Biol Chem 278 (50), 49671-49674 (2003)
   PUBMED   14576168
REFERENCE   9  (bases 1 to 1101)
  AUTHORS   Sato,S., Tomomori-Sato,C., Banks,C.A., Sorokina,I., Parmely,T.J.,
            Kong,S.E., Jin,J., Cai,Y., Lane,W.S., Brower,C.S., Conaway,R.C. and
            Conaway,J.W.
  TITLE     Identification of mammalian Mediator subunits with similarities to
            yeast Mediator subunits Srb5, Srb6, Med11, and Rox3
  JOURNAL   J Biol Chem 278 (17), 15123-15127 (2003)
   PUBMED   12584197
REFERENCE   10 (bases 1 to 1101)
  AUTHORS   Mittler,G., Kremmer,E., Timmers,H.T. and Meisterernst,M.
  TITLE     Novel critical role of a human Mediator complex for basal RNA
            polymerase II transcription
  JOURNAL   EMBO Rep 2 (9), 808-813 (2001)
   PUBMED   11559591
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC093307.5, BC003353.1, BI597082.1, AA648258.1 and AC010635.6.
            
            On Nov 22, 2018 this sequence version replaced NM_032286.2.
            
            Summary: MED10 is a component of the Mediator complex, which is a
            coactivator for DNA-binding factors that activate transcription via
            RNA polymerase II (Sato et al., 2003 [PubMed 12584197]).[supplied
            by OMIM, Oct 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CB149061.1, SRR5189652.30109.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2467146 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000255764.4/ ENSP00000255764.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-21                AC093307.5         50131-50151         c
            22-46               BC003353.1         1-25
            47-178              BI597082.1         15-146
            179-812             BC003353.1         158-791
            813-1049            AA648258.1         32-268              c
            1050-1101           AC010635.6         4978-5029
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5p15.31"
     gene            1..1101
                     /gene="MED10"
                     /gene_synonym="L6; NUT2; TRG20"
                     /note="mediator complex subunit 10"
                     /db_xref="GeneID:84246"
                     /db_xref="HGNC:HGNC:28760"
                     /db_xref="MIM:612382"
     exon            1..186
                     /gene="MED10"
                     /gene_synonym="L6; NUT2; TRG20"
                     /inference="alignment:Splign:2.1.0"
     CDS             65..472
                     /gene="MED10"
                     /gene_synonym="L6; NUT2; TRG20"
                     /note="TRG-17; TRG-20; transformation-related gene 17
                     protein; transformation-related gene 20 protein"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 10"
                     /protein_id="NP_115662.2"
                     /db_xref="CCDS:CCDS34134.1"
                     /db_xref="GeneID:84246"
                     /db_xref="HGNC:HGNC:28760"
                     /db_xref="MIM:612382"
                     /translation="MAEKFDHLEEHLEKFVENIRQLGIIVSDFQPSSQAGLNQKLNFI
                     VTGLQDIDKCRQQLHDITVPLEVFEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTM
                     KKFKSLLIQELSKVFPEDMAKYRSIRGEDHPPS"
     exon            187..270
                     /gene="MED10"
                     /gene_synonym="L6; NUT2; TRG20"
                     /inference="alignment:Splign:2.1.0"
     exon            271..373
                     /gene="MED10"
                     /gene_synonym="L6; NUT2; TRG20"
                     /inference="alignment:Splign:2.1.0"
     exon            374..1101
                     /gene="MED10"
                     /gene_synonym="L6; NUT2; TRG20"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1028..1033
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED10"
                     /gene_synonym="L6; NUT2; TRG20"
                     /note="hexamer: AATAAA"
     polyA_site      1049
                     /gene="MED10"
                     /gene_synonym="L6; NUT2; TRG20"
                     /note="major polyA site"
     regulatory      1075..1080
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MED10"
                     /gene_synonym="L6; NUT2; TRG20"
                     /note="hexamer: AATACA"
     polyA_site      1076
                     /gene="MED10"
                     /gene_synonym="L6; NUT2; TRG20"
     polyA_site      1101
                     /gene="MED10"
                     /gene_synonym="L6; NUT2; TRG20"
ORIGIN      
        1 agcgtagggc cacgcctgcg gcgctgctgg ttgaggctgt gtgggtcggg gacgggccga
       61 ggcgatggcg gagaagtttg accacctaga ggagcacctg gagaagttcg tggagaacat
      121 tcggcagctc ggcatcatcg tcagtgactt ccagcccagc agccaggccg ggctcaacca
      181 aaagctgaat tttattgtta ctggcttaca ggatattgac aagtgcagac agcagcttca
      241 tgatattact gtaccgttag aagtttttga atatatagat caaggtcgaa atccccagct
      301 ctacaccaaa gagtgcctgg agagggctct agctaaaaat gagcaagtta aaggcaagat
      361 cgacaccatg aagaaattta aaagcctgtt gattcaagaa ctttctaaag tatttccgga
      421 agacatggct aagtatcgaa gcatccgggg ggaggatcac ccgccttctt aaccagctca
      481 ccctccctgt gtgaagatcc cctgggactg cgatgcggcg tgaggctggg actgcgagtg
      541 ctgacgccac cttcctgctg aggtgggact gggccctgga cacacccctc agcccctctg
      601 tcctcattgt ttggcctcat gggaccgagg ggctggagga gaggcggagc tgtgccccag
      661 ctgttccagc agcttgtctg gcgtcaactg gctttcagag tgctgacccc tcatcactgt
      721 ggggatcatt ctctctgagg gcagatgagg cgcaggaaaa tagtcttgga aatgttaaat
      781 atgatgggta aattaaaagt tttacaacat tctacctaat atttttcttt taacatactc
      841 tttctgttct attgtattat ggtgtccgaa agctaaataa cgactaggaa aaattttttt
      901 aaaaaaagaa aaatcagttt aatgtgggaa gtacttaagt ggtattatat tttacatttt
      961 caagtatagt gcataaagaa tgttttaaat gtaactgttt tcatggattt caattagaca
     1021 tgcctataat aaactaagta tgtggcttat tccatgtaat tatttttgta atataataca
     1081 atgtgtcttg taaacagtta a
//
