LOCUS       NM_004992              10467 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens methyl-CpG binding protein 2 (MECP2), transcript
            variant 1, mRNA.
ACCESSION   NM_004992
VERSION     NM_004992.4
KEYWORDS    RefSeq; MANE Plus Clinical.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10467)
  AUTHORS   Tomasello,D.L., Barrasa,M.I., Mankus,D., Alarcon,K.I.,
            Lytton-Jean,A.K.R., Liu,X.S. and Jaenisch,R.
  TITLE     Mitochondrial dysfunction and increased reactive oxygen species
            production in MECP2 mutant astrocytes and their impact on neurons
  JOURNAL   Sci Rep 14 (1), 20565 (2024)
   PUBMED   39232000
  REMARK    GeneRIF: Mitochondrial dysfunction and increased reactive oxygen
            species production in MECP2 mutant astrocytes and their impact on
            neurons.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10467)
  AUTHORS   Fichou,Y., Nectoux,J., Bahi-Buisson,N., Rosas-Vargas,H., Girard,B.,
            Chelly,J. and Bienvenu,T.
  TITLE     The first missense mutation causing Rett syndrome specifically
            affecting the MeCP2_e1 isoform
  JOURNAL   Neurogenetics 10 (2), 127-133 (2009)
   PUBMED   19034540
  REMARK    GeneRIF: Data suggest the important involvement of the N-terminus
            in the function of MeCP2 protein, and provide further evidence for
            the major impact of a specific MeCP2e_1 deficiency in the
            development of intellectual processing.
REFERENCE   3  (bases 1 to 10467)
  AUTHORS   Harvey,C.G., Menon,S.D., Stachowiak,B., Noor,A., Proctor,A.,
            Mensah,A.K., Mnatzakanian,G.N., Alfred,S.E., Guo,R., Scherer,S.W.,
            Kennedy,J.L., Roberts,W., Srivastava,A.K., Minassian,B.A. and
            Vincent,J.B.
  TITLE     Sequence variants within exon 1 of MECP2 occur in females with
            mental retardation
  JOURNAL   Am J Med Genet B Neuropsychiatr Genet 144B (3), 355-360 (2007)
   PUBMED   17171659
  REMARK    GeneRIF: polymorphisms within exon 1 of MECP2 occur in females with
            mental retardation.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Erratum:[Am J Med Genet B Neuropsychiatr Genet. 2007 Oct
            5;144(7):969. Srivistava, Anand K [corrected to Srivastava, Anand
            K]]
REFERENCE   4  (bases 1 to 10467)
  AUTHORS   Francke,U.
  TITLE     Mechanisms of disease: neurogenetics of MeCP2 deficiency
  JOURNAL   Nat Clin Pract Neurol 2 (4), 212-221 (2006)
   PUBMED   16932552
  REMARK    GeneRIF: Rett syndrome is caused by heterozygosity for mutations in
            the X-linked gene MECP2, which encodes methyl-CpG binding protein
            2.
            Review article
REFERENCE   5  (bases 1 to 10467)
  AUTHORS   Mnatzakanian,G.N., Lohi,H., Munteanu,I., Alfred,S.E., Yamada,T.,
            MacLeod,P.J., Jones,J.R., Scherer,S.W., Schanen,N.C., Friez,M.J.,
            Vincent,J.B. and Minassian,B.A.
  TITLE     A previously unidentified MECP2 open reading frame defines a new
            protein isoform relevant to Rett syndrome
  JOURNAL   Nat Genet 36 (4), 339-341 (2004)
   PUBMED   15034579
  REMARK    GeneRIF: description of a previously unknown MeCP2 isoform
            Erratum:[Nat Genet. 2004 May;36(5):540]
REFERENCE   6  (bases 1 to 10467)
  AUTHORS   Reichwald,K., Thiesen,J., Wiehe,T., Weitzel,J., Poustka,W.A.,
            Rosenthal,A., Platzer,M., Stratling,W.H. and Kioschis,P.
  TITLE     Comparative sequence analysis of the MECP2-locus in human and mouse
            reveals new transcribed regions
  JOURNAL   Mamm Genome 11 (3), 182-190 (2000)
   PUBMED   10723722
REFERENCE   7  (bases 1 to 10467)
  AUTHORS   Kaur,S. and Christodoulou,J.
  TITLE     MECP2 Disorders
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301670
REFERENCE   8  (bases 1 to 10467)
  AUTHORS   Van Esch,H.
  TITLE     MECP2 Duplication Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301461
REFERENCE   9  (bases 1 to 10467)
  AUTHORS   Klein,C., Lohmann,K., Marras,C. and Munchau,A.
  TITLE     Hereditary Dystonia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301334
REFERENCE   10 (bases 1 to 10467)
  AUTHORS   Lewis,J.D., Meehan,R.R., Henzel,W.J., Maurer-Fogy,I., Jeppesen,P.,
            Klein,F. and Bird,A.
  TITLE     Purification, sequence, and cellular localization of a novel
            chromosomal protein that binds to methylated DNA
  JOURNAL   Cell 69 (6), 905-914 (1992)
   PUBMED   1606614
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC244097.3, AF030876.2 and
            U52112.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 21, 2020 this sequence version replaced NM_004992.3.
            
            Summary: DNA methylation is the major modification of eukaryotic
            genomes and plays an essential role in mammalian development. Human
            proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of
            nuclear proteins related by the presence in each of a methyl-CpG
            binding domain (MBD). Each of these proteins, with the exception of
            MBD3, is capable of binding specifically to methylated DNA. MECP2,
            MBD1 and MBD2 can also repress transcription from methylated gene
            promoters. In contrast to other MBD family members, MECP2 is
            X-linked and subject to X inactivation. MECP2 is dispensible in
            stem cells, but is essential for embryonic development. MECP2 gene
            mutations are the cause of most cases of Rett syndrome, a
            progressive neurologic developmental disorder and one of the most
            common causes of cognitive disability in females. Alternative
            splicing results in multiple transcript variants encoding different
            isoforms. [provided by RefSeq, Oct 2015].
            
            Transcript Variant: This variant (1), also known as MECP2A,
            includes exon 2. Translation is reported to initiate in exon 2
            resulting in a protein isoform (1) with a distinct N-terminus.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF158180.1, HM156732.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2144333, SAMEA2159368
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match :: ENST00000303391.11/ ENSP00000301948.6
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-114               AC244097.3         29110-29223         c
            115-238             AC244097.3         23690-23813         c
            239-589             AF030876.2         23679-24029         c
            590-3630            AF030876.2         19882-22922         c
            3631-10467          U52112.2           174507-181343       c
FEATURES             Location/Qualifiers
     source          1..10467
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq28"
     gene            1..10467
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="methyl-CpG binding protein 2"
                     /db_xref="GeneID:4204"
                     /db_xref="HGNC:HGNC:6990"
                     /db_xref="MIM:300005"
     exon            1..114
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     exon            115..238
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     CDS             213..1673
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="isoform 1 is encoded by transcript variant 1;
                     meCp-2 protein"
                     /codon_start=1
                     /product="methyl-CpG-binding protein 2 isoform 1"
                     /protein_id="NP_004983.1"
                     /db_xref="CCDS:CCDS14741.1"
                     /db_xref="GeneID:4204"
                     /db_xref="HGNC:HGNC:6990"
                     /db_xref="MIM:300005"
                     /translation="MVAGMLGLREEKSEDQDLQGLKDKPLKFKKVKKDKKEEKEGKHE
                     PVQPSAHHSAEPAEAGKAETSEGSGSAPAVPEASASPKQRRSIIRDRGPMYDDPTLPE
                     GWTRKLKQRKSGRSAGKYDVYLINPQGKAFRSKVELIAYFEKVGDTSLDPNDFDFTVT
                     GRGSPSRREQKPPKKPKSPKAPGTGRGRGRPKGSGTTRPKAATSEGVQVKRVLEKSPG
                     KLLVKMPFQTSPGGKAEGGGATTSTQVMVIKRPGRKRKAEADPQAIPKKRGRKPGSVV
                     AAAAAEAKKKAVKESSIRSVQETVLPIKKRKTRETVSIEVKEVVKPLLVSTLGEKSGK
                     GLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHHHHHSESPKAPVPLLPPLPPPPPE
                     PESSEDPTSPPEPQDLSSSVCKEEKMPRGGSLESDGCPKEPAKTQPAVATAATAAEKY
                     KHRGEGERKDIVSSSMPRPNREEPVDSRTPVTERVS"
     exon            239..589
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     exon            590..10467
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10445..10450
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="hexamer: GATAAA"
     polyA_site      10467
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="major polyA site"
ORIGIN      
        1 gcgcgcgctc cctcctctcg gagagagggc tgtggtaaaa gccgtccgga aaatggccgc
       61 cgccgccgcc gccgcgccga gcggaggagg aggaggaggc gaggaggaga gactgctcca
      121 taaaaataca gactcaccag ttcctgcttt gatgtgacat gtgactcccc agaatacacc
      181 ttgcttctgt agaccagctc caacaggatt ccatggtagc tgggatgtta gggctcaggg
      241 aagaaaagtc agaagaccag gacctccagg gcctcaagga caaacccctc aagtttaaaa
      301 aggtgaagaa agataagaaa gaagagaaag agggcaagca tgagcccgtg cagccatcag
      361 cccaccactc tgctgagccc gcagaggcag gcaaagcaga gacatcagaa gggtcaggct
      421 ccgccccggc tgtgccggaa gcttctgcct cccccaaaca gcggcgctcc atcatccgtg
      481 accggggacc catgtatgat gaccccaccc tgcctgaagg ctggacacgg aagcttaagc
      541 aaaggaaatc tggccgctct gctgggaagt atgatgtgta tttgatcaat ccccagggaa
      601 aagcctttcg ctctaaagtg gagttgattg cgtacttcga aaaggtaggc gacacatccc
      661 tggaccctaa tgattttgac ttcacggtaa ctgggagagg gagcccctcc cggcgagagc
      721 agaaaccacc taagaagccc aaatctccca aagctccagg aactggcaga ggccggggac
      781 gccccaaagg gagcggcacc acgagaccca aggcggccac gtcagagggt gtgcaggtga
      841 aaagggtcct ggagaaaagt cctgggaagc tccttgtcaa gatgcctttt caaacttcgc
      901 cagggggcaa ggctgagggg ggtggggcca ccacatccac ccaggtcatg gtgatcaaac
      961 gccccggcag gaagcgaaaa gctgaggccg accctcaggc cattcccaag aaacggggcc
     1021 gaaagccggg gagtgtggtg gcagccgctg ccgccgaggc caaaaagaaa gccgtgaagg
     1081 agtcttctat ccgatctgtg caggagaccg tactccccat caagaagcgc aagacccggg
     1141 agacggtcag catcgaggtc aaggaagtgg tgaagcccct gctggtgtcc accctcggtg
     1201 agaagagcgg gaaaggactg aagacctgta agagccctgg gcggaaaagc aaggagagca
     1261 gccccaaggg gcgcagcagc agcgcctcct caccccccaa gaaggagcac caccaccatc
     1321 accaccactc agagtcccca aaggcccccg tgccactgct cccacccctg cccccacctc
     1381 cacctgagcc cgagagctcc gaggacccca ccagcccccc tgagccccag gacttgagca
     1441 gcagcgtctg caaagaggag aagatgccca gaggaggctc actggagagc gacggctgcc
     1501 ccaaggagcc agctaagact cagcccgcgg ttgccaccgc cgccacggcc gcagaaaagt
     1561 acaaacaccg aggggaggga gagcgcaaag acattgtttc atcctccatg ccaaggccaa
     1621 acagagagga gcctgtggac agccggacgc ccgtgaccga gagagttagc tgactttaca
     1681 cggagcggat tgcaaagcaa accaacaaga ataaaggcag ctgttgtctc ttctccttat
     1741 gggtagggct ctgacaaagc ttcccgatta actgaaataa aaaatatttt tttttctttc
     1801 agtaaactta gagtttcgtg gcttcagggt gggagtagtt ggagcattgg ggatgttttt
     1861 cttaccgaca agcacagtca ggttgaagac ctaaccaggg ccagaagtag ctttgcactt
     1921 ttctaaacta ggctccttca acaaggcttg ctgcagatac tactgaccag acaagctgtt
     1981 gaccaggcac ctcccctccc gcccaaacct ttcccccatg tggtcgttag agacagagcg
     2041 acagagcagt tgagaggaca ctcccgtttt cggtgccatc agtgccccgt ctacagctcc
     2101 cccagctccc cccacctccc ccactcccaa ccacgttggg acagggaggt gtgaggcagg
     2161 agagacagtt ggattcttta gagaagatgg atatgaccag tggctatggc ctgtgcgatc
     2221 ccacccgtgg tggctcaagt ctggccccac accagcccca atccaaaact ggcaaggacg
     2281 cttcacagga caggaaagtg gcacctgtct gctccagctc tggcatggct aggagggggg
     2341 agtcccttga actactgggt gtagactggc ctgaaccaca ggagaggatg gcccagggtg
     2401 aggtggcatg gtccattctc aagggacgtc ctccaacggg tggcgctaga ggccatggag
     2461 gcagtaggac aaggtgcagg caggctggcc tggggtcagg ccgggcagag cacagcgggg
     2521 tgagagggat tcctaatcac tcagagcagt ctgtgactta gtggacaggg gagggggcaa
     2581 agggggagga gaagaaaatg ttcttccagt tactttccaa ttctccttta gggacagctt
     2641 agaattattt gcactattga gtcttcatgt tcccacttca aaacaaacag atgctctgag
     2701 agcaaactgg cttgaattgg tgacatttag tccctcaagc caccagatgt gacagtgttg
     2761 agaactacct ggatttgtat atatacctgc gcttgtttta aagtgggctc agcacatagg
     2821 gttcccacga agctccgaaa ctctaagtgt ttgctgcaat tttataagga cttcctgatt
     2881 ggtttctctt ctccccttcc atttctgcct tttgttcatt tcatcctttc acttctttcc
     2941 cttcctccat cctcctcctt cctagttcat cccttctctt ccaggcagcc gcggtgccca
     3001 accacacttg tcggctccag tccccagaac tctgcctgcc ctttgtcctc ctgctgccag
     3061 taccagcccc accctgtttt gagccctgag gaggccttgg gctctgctga gtccgacctg
     3121 gcctgtctgt gaagagcaag agagcagcaa ggtcttgctc tcctaggtag ccccctcttc
     3181 cctggtaaga aaaagcaaaa ggcatttccc accctgaaca acgagccttt tcacccttct
     3241 actctagaga agtggactgg aggagctggg cccgatttgg tagttgagga aagcacagag
     3301 gcctcctgtg gcctgccagt catcgagtgg cccaacaggg gctccatgcc agccgacctt
     3361 gacctcactc agaagtccag agtctagcgt agtgcagcag ggcagtagcg gtaccaatgc
     3421 agaactccca agacccgagc tgggaccagt acctgggtcc ccagcccttc ctctgctccc
     3481 ccttttccct cggagttctt cttgaatggc aatgttttgc ttttgctcga tgcagacagg
     3541 gggccagaac accacacatt tcactgtctg tctggtccat agctgtggtg taggggctta
     3601 gaggcatggg cttgctgtgg gtttttaatt gatcagtttt catgtgggat cccatctttt
     3661 taacctctgt tcaggaagtc cttatctagc tgcatatctt catcatattg gtatatcctt
     3721 ttctgtgttt acagagatgt ctcttatatc taaatctgtc caactgagaa gtaccttatc
     3781 aaagtagcaa atgagacagc agtcttatgc ttccagaaac acccacaggc atgtcccatg
     3841 tgagctgctg ccatgaactg tcaagtgtgt gttgtcttgt gtatttcagt tattgtccct
     3901 ggcttcctta ctatggtgta atcatgaagg agtgaaacat catagaaact gtctagcact
     3961 tccttgccag tctttagtga tcaggaacca tagttgacag ttccaatcag tagcttaaga
     4021 aaaaaccgtg tttgtctctt ctggaatggt tagaagtgag ggagtttgcc ccgttctgtt
     4081 tgtagagtct catagttgga ctttctagca tatatgtgtc catttcctta tgctgtaaaa
     4141 gcaagtcctg caaccaaact cccatcagcc caatccctga tccctgatcc cttccacctg
     4201 ctctgctgat gaccccccca gcttcacttc tgactcttcc ccaggaaggg aaggggggtc
     4261 agaagagagg gtgagtcctc cagaactctt cctccaagga cagaaggctc ctgcccccat
     4321 agtggcctcg aactcctggc actaccaaag gacacttatc cacgagagcg cagcatccga
     4381 ccaggttgtc actgagaaga tgtttatttt ggtcagttgg gtttttatgt attatactta
     4441 gtcaaatgta atgtggcttc tggaatcatt gtccagagct gcttccccgt cacctgggcg
     4501 tcatctggtc ctggtaagag gagtgcgtgg cccaccaggc ccccctgtca cccatgacag
     4561 ttcattcagg gccgatgggg cagtcgtggt tgggaacaca gcatttcaag cgtcacttta
     4621 tttcattcgg gccccacctg cagctccctc aaagaggcag ttgcccagcc tctttccctt
     4681 ccagtttatt ccagagctgc cagtggggcc tgaggctcct tagggttttc tctctatttc
     4741 cccctttctt cctcattccc tcgtctttcc caaaggcatc acgagtcagt cgcctttcag
     4801 caggcagcct tggcggttta tcgccctggc aggcaggggc cctgcagctc tcatgctgcc
     4861 cctgccttgg ggtcaggttg acaggaggtt ggagggaaag ccttaagctg caggattctc
     4921 accagctgtg tccggcccag ttttggggtg tgacctcaat ttcaattttg tctgtacttg
     4981 aacattatga agatgggggc ctctttcagt gaatttgtga acagcagaat tgaccgacag
     5041 ctttccagta cccatggggc taggtcatta aggccacatc cacagtctcc cccacccttg
     5101 ttccagttgt tagttactac ctcctctcct gacaatactg tatgtcgtcg agctcccccc
     5161 aggtctaccc ctcccggccc tgcctgctgg tgggcttgtc atagccagtg ggattgccgg
     5221 tcttgacagc tcagtgagct ggagatactt ggtcacagcc aggcgctagc acagctccct
     5281 tctgttgatg ctgtattccc atatcaaaag acacagggga cacccagaaa cgccacatcc
     5341 cccaatccat cagtgccaaa ctagccaacg gccccagctt ctcagctcgc tggatggcgg
     5401 aagctgctac tcgtgagcgc cagtgcgggt gcagacaatc ttctgttggg tggcatcatt
     5461 ccaggcccga agcatgaaca gtgcacctgg gacagggagc agccccaaat tgtcacctgc
     5521 ttctctgccc agcttttcat tgctgtgaca gtgatggcga aagagggtaa taaccagaca
     5581 caaactgcca agttgggtgg agaaaggagt ttctttagct gacagaatct ctgaatttta
     5641 aatcacttag taagcggctc aagcccagga gggagcagag ggatacgagc ggagtcccct
     5701 gcgcgggacc atctggaatt ggtttagccc aagtggagcc tgacagccag aactctgtgt
     5761 cccccgtcta accacagctc cttttccaga gcattccagt caggctctct gggctgactg
     5821 ggccagggga ggttacaggt accagttctt taagaagatc tttgggcata tacattttta
     5881 gcctgtgtca ttgccccaaa tggattcctg tttcaagttc acacctgcag attctaggac
     5941 ctgtgtccta gacttcaggg agtcagctgt ttctagagtt cctaccatgg agtgggtctg
     6001 gaggacctgc ccggtggggg ggcagagccc tgctccctcc gggtcttcct actcttctct
     6061 ctgctctgac gggatttgtt gattctctcc attttggtgt ctttctcttt tagatattgt
     6121 atcaatcttt agaaaaggca tagtctactt gttataaatc gttaggatac tgcctccccc
     6181 agggtctaaa attacatatt agaggggaaa agctgaacac tgaagtcagt tctcaacaat
     6241 ttagaaggaa aacctagaaa acatttggca gaaaattaca tttcgatgtt tttgaatgaa
     6301 tacgagcaag cttttacaac agtgctgatc taaaaatact tagcacttgg cctgagatgc
     6361 ctggtgagca ttacaggcaa ggggaatctg gaggtagccg acctgaggac atggcttctg
     6421 aacctgtctt ttgggagtgg tatggaaggt ggagcgttca ccagtgacct ggaaggccca
     6481 gcaccaccct ccttcccact cttctcatct tgacagagcc tgccccagcg ctgacgtgtc
     6541 aggaaaacac ccagggaact aggaaggcac ttctgcctga ggggcagcct gccttgccca
     6601 ctcctgctct gctcgcctcg gatcagctga gccttctgag ctggcctctc actgcctccc
     6661 caaggccccc tgcctgccct gtcaggaggc agaaggaagc aggtgtgagg gcagtgcaag
     6721 gagggagcac aacccccagc tcccgctccg ggctccgact tgtgcacagg cagagcccag
     6781 accctggagg aaatcctacc tttgaattca agaacatttg gggaatttgg aaatctcttt
     6841 gcccccaaac ccccattctg tcctaccttt aatcaggtcc tgctcagcag tgagagcaga
     6901 tgaggtgaaa aggccaagag gtttggctcc tgcccactga tagcccctct ccccgcagtg
     6961 tttgtgtgtc aagtggcaaa gctgttcttc ctggtgaccc tgattatatc cagtaacaca
     7021 tagactgtgc gcataggcct gctttgtctc ctctatcctg ggcttttgtt ttgcttttta
     7081 gttttgcttt tagtttttct gtccctttta tttaacgcac cgactagaca cacaaagcag
     7141 ttgaattttt atatatatat ctgtatattg cacaattata aactcatttt gcttgtggct
     7201 ccacacacac aaaaaaagac ctgttaaaat tatacctgtt gcttaattac aatatttctg
     7261 ataaccatag cataggacaa gggaaaataa aaaaagaaaa aaaagaaaaa aaaacgacaa
     7321 atctgtctgc tggtcacttc ttctgtccaa gcagattcgt ggtcttttcc tcgcttcttt
     7381 caagggcttt cctgtgccag gtgaaggagg ctccaggcag cacccaggtt ttgcactctt
     7441 gtttctcccg tgcttgtgaa agaggtccca aggttctggg tgcaggagcg ctcccttgac
     7501 ctgctgaagt ccggaacgta gtcggcacag cctggtcgcc ttccacctct gggagctgga
     7561 gtccactggg gtggcctgac tcccccagtc cccttcccgt gacctggtca gggtgagccc
     7621 atgtggagtc agcctcgcag gcctccctgc cagtagggtc cgagtgtgtt tcatccttcc
     7681 cactctgtcg agcctggggg ctggagcgga gacgggaggc ctggcctgtc tcggaacctg
     7741 tgagctgcac caggtagaac gccagggacc ccagaatcat gtgcgtcagt ccaaggggtc
     7801 ccctccagga gtagtgaaga ctccagaaat gtccctttct tctcccccat cctacgagta
     7861 attgcatttg cttttgtaat tcttaatgag caatatctgc tagagagttt agctgtaaca
     7921 gttctttttg atcatctttt tttaataatt agaaacacca aaaaaatcca gaaacttgtt
     7981 cttccaaagc agagagcatt ataatcacca gggccaaaag cttccctccc tgctgtcatt
     8041 gcttcttctg aggcctgaat ccaaaagaaa aacagccata ggccctttca gtggccgggc
     8101 tacccgtgag cccttcggag gaccagggct ggggcagcct ctgggcccac atccggggcc
     8161 agctccggcg tgtgttcagt gttagcagtg ggtcatgatg ctctttccca cccagcctgg
     8221 gataggggca gaggaggcga ggaggccgtt gccgctgatg tttggccgtg aacaggtggg
     8281 tgtctgcgtg cgtccacgtg cgtgttttct gactgacatg aaatcgacgc ccgagttagc
     8341 ctcacccggt gacctctagc cctgcccgga tggagcgggg cccacccggt tcagtgtttc
     8401 tggggagctg gacagtggag tgcaaaaggc ttgcagaact tgaagcctgc tccttccctt
     8461 gctaccacgg cctcctttcc gtttgatttg tcactgcttc aatcaataac agccgctcca
     8521 gagtcagtag tcaatgaata tatgaccaaa tatcaccagg actgttactc aatgtgtgcc
     8581 gagcccttgc ccatgctggg ctcccgtgta tctggacact gtaacgtgtg ctgtgtttgc
     8641 tccccttccc cttccttctt tgccctttac ttgtctttct ggggtttttc tgtttgggtt
     8701 tggtttggtt tttatttctc cttttgtgtt ccaaacatga ggttctctct actggtcctc
     8761 ttaactgtgg tgttgaggct tatatttgtg taatttttgg tgggtgaaag gaattttgct
     8821 aagtaaatct cttctgtgtt tgaactgaag tctgtattgt aactatgttt aaagtaattg
     8881 ttccagagac aaatatttct agacactttt tctttacaaa caaaagcatt cggagggagg
     8941 gggatggtga ctgagatgag aggggagagc tgaacagatg acccctgccc agatcagcca
     9001 gaagccaccc aaagcagtgg agcccaggag tcccactcca agccagcaag ccgaatagct
     9061 gatgtgttgc cactttccaa gtcactgcaa aaccaggttt tgttccgccc agtggattct
     9121 tgttttgctt cccctccccc cgagattatt accaccatcc cgtgctttta aggaaaggca
     9181 agattgatgt ttccttgagg ggagccagga ggggatgtgt gtgtgcagag ctgaagagct
     9241 ggggagaatg gggctgggcc cacccaagca ggaggctggg acgctctgct gtgggcacag
     9301 gtcaggctaa tgttggcaga tgcagctctt cctggacagg ccaggtggtg ggcattctct
     9361 ctccaaggtg tgccccgtgg gcattactgt ttaagacact tccgtcacat cccaccccat
     9421 cctccagggc tcaacactgt gacatctcta ttccccaccc tccccttccc agggcaataa
     9481 aatgaccatg gagggggctt gcactctctt ggctgtcacc cgatcgccag caaaacttag
     9541 atgtgagaaa accccttccc attccatggc gaaaacatct ccttagaaaa gccattaccc
     9601 tcattaggca tggttttggg ctcccaaaac acctgacagc ccctccctcc tctgagaggc
     9661 ggagagtgct gactgtagtg accattgcat gccgggtgca gcatctggaa gagctaggca
     9721 gggtgtctgc cccctcctga gttgaagtca tgctcccctg tgccagccca gaggccgaga
     9781 gctatggaca gcattgccag taacacaggc caccctgtgc agaagggagc tggctccagc
     9841 ctggaaacct gtctgaggtt gggagaggtg cacttggggc acagggagag gccgggacac
     9901 acttagctgg agatgtctct aaaagccctg tatcgtattc accttcagtt tttgtgtttt
     9961 gggacaatta ctttagaaaa taagtaggtc gttttaaaaa caaaaattat tgattgcttt
    10021 tttgtagtgt tcagaaaaaa ggttctttgt gtatagccaa atgactgaaa gcactgatat
    10081 atttaaaaac aaaaggcaat ttattaagga aatttgtacc atttcagtaa acctgtctga
    10141 atgtacctgt atacgtttca aaaacacccc ccccccactg aatccctgta acctatttat
    10201 tatataaaga gtttgcctta taaatttaca taaaaatgtc cgtttgtgtc ttttgttgta
    10261 aaaatcaagt gattttttca taaggttctt ttactattgg aaaagatggg cagcacgcag
    10321 ttttatttta tttttgtaag ttttttaata catgtgaaag caaagaatac tcagcatgcc
    10381 tttctaagtg acgcgtttgc accttttgtt gggaagtact gtatcctgtg ctgttagcat
    10441 tctcgataaa tctctctgtg aaagtga
//
