LOCUS       NM_001386139           10234 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens methyl-CpG binding protein 2 (MECP2), transcript
            variant 10, mRNA.
ACCESSION   NM_001386139
VERSION     NM_001386139.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10234)
  AUTHORS   Tomasello,D.L., Barrasa,M.I., Mankus,D., Alarcon,K.I.,
            Lytton-Jean,A.K.R., Liu,X.S. and Jaenisch,R.
  TITLE     Mitochondrial dysfunction and increased reactive oxygen species
            production in MECP2 mutant astrocytes and their impact on neurons
  JOURNAL   Sci Rep 14 (1), 20565 (2024)
   PUBMED   39232000
  REMARK    GeneRIF: Mitochondrial dysfunction and increased reactive oxygen
            species production in MECP2 mutant astrocytes and their impact on
            neurons.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10234)
  AUTHORS   Fichou,Y., Nectoux,J., Bahi-Buisson,N., Rosas-Vargas,H., Girard,B.,
            Chelly,J. and Bienvenu,T.
  TITLE     The first missense mutation causing Rett syndrome specifically
            affecting the MeCP2_e1 isoform
  JOURNAL   Neurogenetics 10 (2), 127-133 (2009)
   PUBMED   19034540
  REMARK    GeneRIF: Data suggest the important involvement of the N-terminus
            in the function of MeCP2 protein, and provide further evidence for
            the major impact of a specific MeCP2e_1 deficiency in the
            development of intellectual processing.
REFERENCE   3  (bases 1 to 10234)
  AUTHORS   Harvey,C.G., Menon,S.D., Stachowiak,B., Noor,A., Proctor,A.,
            Mensah,A.K., Mnatzakanian,G.N., Alfred,S.E., Guo,R., Scherer,S.W.,
            Kennedy,J.L., Roberts,W., Srivastava,A.K., Minassian,B.A. and
            Vincent,J.B.
  TITLE     Sequence variants within exon 1 of MECP2 occur in females with
            mental retardation
  JOURNAL   Am J Med Genet B Neuropsychiatr Genet 144B (3), 355-360 (2007)
   PUBMED   17171659
  REMARK    GeneRIF: polymorphisms within exon 1 of MECP2 occur in females with
            mental retardation.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Erratum:[Am J Med Genet B Neuropsychiatr Genet. 2007 Oct
            5;144(7):969. Srivistava, Anand K [corrected to Srivastava, Anand
            K]]
REFERENCE   4  (bases 1 to 10234)
  AUTHORS   Francke,U.
  TITLE     Mechanisms of disease: neurogenetics of MeCP2 deficiency
  JOURNAL   Nat Clin Pract Neurol 2 (4), 212-221 (2006)
   PUBMED   16932552
  REMARK    GeneRIF: Rett syndrome is caused by heterozygosity for mutations in
            the X-linked gene MECP2, which encodes methyl-CpG binding protein
            2.
            Review article
REFERENCE   5  (bases 1 to 10234)
  AUTHORS   Mnatzakanian,G.N., Lohi,H., Munteanu,I., Alfred,S.E., Yamada,T.,
            MacLeod,P.J., Jones,J.R., Scherer,S.W., Schanen,N.C., Friez,M.J.,
            Vincent,J.B. and Minassian,B.A.
  TITLE     A previously unidentified MECP2 open reading frame defines a new
            protein isoform relevant to Rett syndrome
  JOURNAL   Nat Genet 36 (4), 339-341 (2004)
   PUBMED   15034579
  REMARK    GeneRIF: description of a previously unknown MeCP2 isoform
            Erratum:[Nat Genet. 2004 May;36(5):540]
REFERENCE   6  (bases 1 to 10234)
  AUTHORS   Reichwald,K., Thiesen,J., Wiehe,T., Weitzel,J., Poustka,W.A.,
            Rosenthal,A., Platzer,M., Stratling,W.H. and Kioschis,P.
  TITLE     Comparative sequence analysis of the MECP2-locus in human and mouse
            reveals new transcribed regions
  JOURNAL   Mamm Genome 11 (3), 182-190 (2000)
   PUBMED   10723722
REFERENCE   7  (bases 1 to 10234)
  AUTHORS   Kaur,S. and Christodoulou,J.
  TITLE     MECP2 Disorders
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301670
REFERENCE   8  (bases 1 to 10234)
  AUTHORS   Van Esch,H.
  TITLE     MECP2 Duplication Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301461
REFERENCE   9  (bases 1 to 10234)
  AUTHORS   Klein,C., Lohmann,K., Marras,C. and Munchau,A.
  TITLE     Hereditary Dystonia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301334
REFERENCE   10 (bases 1 to 10234)
  AUTHORS   Lewis,J.D., Meehan,R.R., Henzel,W.J., Maurer-Fogy,I., Jeppesen,P.,
            Klein,F. and Bird,A.
  TITLE     Purification, sequence, and cellular localization of a novel
            chromosomal protein that binds to methylated DNA
  JOURNAL   Cell 69 (6), 905-914 (1992)
   PUBMED   1606614
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC244097.3, AF030876.2 and
            U52112.2.
            
            Summary: DNA methylation is the major modification of eukaryotic
            genomes and plays an essential role in mammalian development. Human
            proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of
            nuclear proteins related by the presence in each of a methyl-CpG
            binding domain (MBD). Each of these proteins, with the exception of
            MBD3, is capable of binding specifically to methylated DNA. MECP2,
            MBD1 and MBD2 can also repress transcription from methylated gene
            promoters. In contrast to other MBD family members, MECP2 is
            X-linked and subject to X inactivation. MECP2 is dispensible in
            stem cells, but is essential for embryonic development. MECP2 gene
            mutations are the cause of most cases of Rett syndrome, a
            progressive neurologic developmental disorder and one of the most
            common causes of cognitive disability in females. Alternative
            splicing results in multiple transcript variants encoding different
            isoforms. [provided by RefSeq, Oct 2015].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038193.462925.1,
                                           SRR14372079.198333.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-114               AC244097.3         29110-29223         c
            115-465             AF030876.2         23679-24029         c
            466-520             AF030876.2         22868-22922         c
            521-3397            AF030876.2         19882-22758         c
            3398-10234          U52112.2           174507-181343       c
FEATURES             Location/Qualifiers
     source          1..10234
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq28"
     gene            1..10234
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="methyl-CpG binding protein 2"
                     /db_xref="GeneID:4204"
                     /db_xref="HGNC:HGNC:6990"
                     /db_xref="MIM:300005"
     exon            1..114
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     exon            115..465
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     exon            466..520
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     exon            521..10234
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     CDS             649..1440
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="isoform 4 is encoded by transcript variant 10;
                     meCp-2 protein"
                     /codon_start=1
                     /product="methyl-CpG-binding protein 2 isoform 4"
                     /protein_id="NP_001373068.1"
                     /db_xref="GeneID:4204"
                     /db_xref="HGNC:HGNC:6990"
                     /db_xref="MIM:300005"
                     /translation="MPFQTSPGGKAEGGGATTSTQVMVIKRPGRKRKAEADPQAIPKK
                     RGRKPGSVVAAAAAEAKKKAVKESSIRSVQETVLPIKKRKTRETVSIEVKEVVKPLLV
                     STLGEKSGKGLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHHHHHSESPKAPVPLL
                     PPLPPPPPEPESSEDPTSPPEPQDLSSSVCKEEKMPRGGSLESDGCPKEPAKTQPAVA
                     TAATAAEKYKHRGEGERKDIVSSSMPRPNREEPVDSRTPVTERVS"
     regulatory      10212..10217
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="hexamer: GATAAA"
     polyA_site      10234
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="major polyA site"
ORIGIN      
        1 gcgcgcgctc cctcctctcg gagagagggc tgtggtaaaa gccgtccgga aaatggccgc
       61 cgccgccgcc gccgcgccga gcggaggagg aggaggaggc gaggaggaga gactggaaga
      121 aaagtcagaa gaccaggacc tccagggcct caaggacaaa cccctcaagt ttaaaaaggt
      181 gaagaaagat aagaaagaag agaaagaggg caagcatgag cccgtgcagc catcagccca
      241 ccactctgct gagcccgcag aggcaggcaa agcagagaca tcagaagggt caggctccgc
      301 cccggctgtg ccggaagctt ctgcctcccc caaacagcgg cgctccatca tccgtgaccg
      361 gggacccatg tatgatgacc ccaccctgcc tgaaggctgg acacggaagc ttaagcaaag
      421 gaaatctggc cgctctgctg ggaagtatga tgtgtatttg atcaatcccc agggaaaagc
      481 ctttcgctct aaagtggagt tgattgcgta cttcgaaaag ctccaggaac tggcagaggc
      541 cggggacgcc ccaaagggag cggcaccacg agacccaagg cggccacgtc agagggtgtg
      601 caggtgaaaa gggtcctgga gaaaagtcct gggaagctcc ttgtcaagat gccttttcaa
      661 acttcgccag ggggcaaggc tgaggggggt ggggccacca catccaccca ggtcatggtg
      721 atcaaacgcc ccggcaggaa gcgaaaagct gaggccgacc ctcaggccat tcccaagaaa
      781 cggggccgaa agccggggag tgtggtggca gccgctgccg ccgaggccaa aaagaaagcc
      841 gtgaaggagt cttctatccg atctgtgcag gagaccgtac tccccatcaa gaagcgcaag
      901 acccgggaga cggtcagcat cgaggtcaag gaagtggtga agcccctgct ggtgtccacc
      961 ctcggtgaga agagcgggaa aggactgaag acctgtaaga gccctgggcg gaaaagcaag
     1021 gagagcagcc ccaaggggcg cagcagcagc gcctcctcac cccccaagaa ggagcaccac
     1081 caccatcacc accactcaga gtccccaaag gcccccgtgc cactgctccc acccctgccc
     1141 ccacctccac ctgagcccga gagctccgag gaccccacca gcccccctga gccccaggac
     1201 ttgagcagca gcgtctgcaa agaggagaag atgcccagag gaggctcact ggagagcgac
     1261 ggctgcccca aggagccagc taagactcag cccgcggttg ccaccgccgc cacggccgca
     1321 gaaaagtaca aacaccgagg ggagggagag cgcaaagaca ttgtttcatc ctccatgcca
     1381 aggccaaaca gagaggagcc tgtggacagc cggacgcccg tgaccgagag agttagctga
     1441 ctttacacgg agcggattgc aaagcaaacc aacaagaata aaggcagctg ttgtctcttc
     1501 tccttatggg tagggctctg acaaagcttc ccgattaact gaaataaaaa atattttttt
     1561 ttctttcagt aaacttagag tttcgtggct tcagggtggg agtagttgga gcattgggga
     1621 tgtttttctt accgacaagc acagtcaggt tgaagaccta accagggcca gaagtagctt
     1681 tgcacttttc taaactaggc tccttcaaca aggcttgctg cagatactac tgaccagaca
     1741 agctgttgac caggcacctc ccctcccgcc caaacctttc ccccatgtgg tcgttagaga
     1801 cagagcgaca gagcagttga gaggacactc ccgttttcgg tgccatcagt gccccgtcta
     1861 cagctccccc agctcccccc acctccccca ctcccaacca cgttgggaca gggaggtgtg
     1921 aggcaggaga gacagttgga ttctttagag aagatggata tgaccagtgg ctatggcctg
     1981 tgcgatccca cccgtggtgg ctcaagtctg gccccacacc agccccaatc caaaactggc
     2041 aaggacgctt cacaggacag gaaagtggca cctgtctgct ccagctctgg catggctagg
     2101 aggggggagt cccttgaact actgggtgta gactggcctg aaccacagga gaggatggcc
     2161 cagggtgagg tggcatggtc cattctcaag ggacgtcctc caacgggtgg cgctagaggc
     2221 catggaggca gtaggacaag gtgcaggcag gctggcctgg ggtcaggccg ggcagagcac
     2281 agcggggtga gagggattcc taatcactca gagcagtctg tgacttagtg gacaggggag
     2341 ggggcaaagg gggaggagaa gaaaatgttc ttccagttac tttccaattc tcctttaggg
     2401 acagcttaga attatttgca ctattgagtc ttcatgttcc cacttcaaaa caaacagatg
     2461 ctctgagagc aaactggctt gaattggtga catttagtcc ctcaagccac cagatgtgac
     2521 agtgttgaga actacctgga tttgtatata tacctgcgct tgttttaaag tgggctcagc
     2581 acatagggtt cccacgaagc tccgaaactc taagtgtttg ctgcaatttt ataaggactt
     2641 cctgattggt ttctcttctc cccttccatt tctgcctttt gttcatttca tcctttcact
     2701 tctttccctt cctccatcct cctccttcct agttcatccc ttctcttcca ggcagccgcg
     2761 gtgcccaacc acacttgtcg gctccagtcc ccagaactct gcctgccctt tgtcctcctg
     2821 ctgccagtac cagccccacc ctgttttgag ccctgaggag gccttgggct ctgctgagtc
     2881 cgacctggcc tgtctgtgaa gagcaagaga gcagcaaggt cttgctctcc taggtagccc
     2941 cctcttccct ggtaagaaaa agcaaaaggc atttcccacc ctgaacaacg agccttttca
     3001 cccttctact ctagagaagt ggactggagg agctgggccc gatttggtag ttgaggaaag
     3061 cacagaggcc tcctgtggcc tgccagtcat cgagtggccc aacaggggct ccatgccagc
     3121 cgaccttgac ctcactcaga agtccagagt ctagcgtagt gcagcagggc agtagcggta
     3181 ccaatgcaga actcccaaga cccgagctgg gaccagtacc tgggtcccca gcccttcctc
     3241 tgctccccct tttccctcgg agttcttctt gaatggcaat gttttgcttt tgctcgatgc
     3301 agacaggggg ccagaacacc acacatttca ctgtctgtct ggtccatagc tgtggtgtag
     3361 gggcttagag gcatgggctt gctgtgggtt tttaattgat cagttttcat gtgggatccc
     3421 atctttttaa cctctgttca ggaagtcctt atctagctgc atatcttcat catattggta
     3481 tatccttttc tgtgtttaca gagatgtctc ttatatctaa atctgtccaa ctgagaagta
     3541 ccttatcaaa gtagcaaatg agacagcagt cttatgcttc cagaaacacc cacaggcatg
     3601 tcccatgtga gctgctgcca tgaactgtca agtgtgtgtt gtcttgtgta tttcagttat
     3661 tgtccctggc ttccttacta tggtgtaatc atgaaggagt gaaacatcat agaaactgtc
     3721 tagcacttcc ttgccagtct ttagtgatca ggaaccatag ttgacagttc caatcagtag
     3781 cttaagaaaa aaccgtgttt gtctcttctg gaatggttag aagtgaggga gtttgccccg
     3841 ttctgtttgt agagtctcat agttggactt tctagcatat atgtgtccat ttccttatgc
     3901 tgtaaaagca agtcctgcaa ccaaactccc atcagcccaa tccctgatcc ctgatccctt
     3961 ccacctgctc tgctgatgac ccccccagct tcacttctga ctcttcccca ggaagggaag
     4021 gggggtcaga agagagggtg agtcctccag aactcttcct ccaaggacag aaggctcctg
     4081 cccccatagt ggcctcgaac tcctggcact accaaaggac acttatccac gagagcgcag
     4141 catccgacca ggttgtcact gagaagatgt ttattttggt cagttgggtt tttatgtatt
     4201 atacttagtc aaatgtaatg tggcttctgg aatcattgtc cagagctgct tccccgtcac
     4261 ctgggcgtca tctggtcctg gtaagaggag tgcgtggccc accaggcccc cctgtcaccc
     4321 atgacagttc attcagggcc gatggggcag tcgtggttgg gaacacagca tttcaagcgt
     4381 cactttattt cattcgggcc ccacctgcag ctccctcaaa gaggcagttg cccagcctct
     4441 ttcccttcca gtttattcca gagctgccag tggggcctga ggctccttag ggttttctct
     4501 ctatttcccc ctttcttcct cattccctcg tctttcccaa aggcatcacg agtcagtcgc
     4561 ctttcagcag gcagccttgg cggtttatcg ccctggcagg caggggccct gcagctctca
     4621 tgctgcccct gccttggggt caggttgaca ggaggttgga gggaaagcct taagctgcag
     4681 gattctcacc agctgtgtcc ggcccagttt tggggtgtga cctcaatttc aattttgtct
     4741 gtacttgaac attatgaaga tgggggcctc tttcagtgaa tttgtgaaca gcagaattga
     4801 ccgacagctt tccagtaccc atggggctag gtcattaagg ccacatccac agtctccccc
     4861 acccttgttc cagttgttag ttactacctc ctctcctgac aatactgtat gtcgtcgagc
     4921 tccccccagg tctacccctc ccggccctgc ctgctggtgg gcttgtcata gccagtggga
     4981 ttgccggtct tgacagctca gtgagctgga gatacttggt cacagccagg cgctagcaca
     5041 gctcccttct gttgatgctg tattcccata tcaaaagaca caggggacac ccagaaacgc
     5101 cacatccccc aatccatcag tgccaaacta gccaacggcc ccagcttctc agctcgctgg
     5161 atggcggaag ctgctactcg tgagcgccag tgcgggtgca gacaatcttc tgttgggtgg
     5221 catcattcca ggcccgaagc atgaacagtg cacctgggac agggagcagc cccaaattgt
     5281 cacctgcttc tctgcccagc ttttcattgc tgtgacagtg atggcgaaag agggtaataa
     5341 ccagacacaa actgccaagt tgggtggaga aaggagtttc tttagctgac agaatctctg
     5401 aattttaaat cacttagtaa gcggctcaag cccaggaggg agcagaggga tacgagcgga
     5461 gtcccctgcg cgggaccatc tggaattggt ttagcccaag tggagcctga cagccagaac
     5521 tctgtgtccc ccgtctaacc acagctcctt ttccagagca ttccagtcag gctctctggg
     5581 ctgactgggc caggggaggt tacaggtacc agttctttaa gaagatcttt gggcatatac
     5641 atttttagcc tgtgtcattg ccccaaatgg attcctgttt caagttcaca cctgcagatt
     5701 ctaggacctg tgtcctagac ttcagggagt cagctgtttc tagagttcct accatggagt
     5761 gggtctggag gacctgcccg gtgggggggc agagccctgc tccctccggg tcttcctact
     5821 cttctctctg ctctgacggg atttgttgat tctctccatt ttggtgtctt tctcttttag
     5881 atattgtatc aatctttaga aaaggcatag tctacttgtt ataaatcgtt aggatactgc
     5941 ctcccccagg gtctaaaatt acatattaga ggggaaaagc tgaacactga agtcagttct
     6001 caacaattta gaaggaaaac ctagaaaaca tttggcagaa aattacattt cgatgttttt
     6061 gaatgaatac gagcaagctt ttacaacagt gctgatctaa aaatacttag cacttggcct
     6121 gagatgcctg gtgagcatta caggcaaggg gaatctggag gtagccgacc tgaggacatg
     6181 gcttctgaac ctgtcttttg ggagtggtat ggaaggtgga gcgttcacca gtgacctgga
     6241 aggcccagca ccaccctcct tcccactctt ctcatcttga cagagcctgc cccagcgctg
     6301 acgtgtcagg aaaacaccca gggaactagg aaggcacttc tgcctgaggg gcagcctgcc
     6361 ttgcccactc ctgctctgct cgcctcggat cagctgagcc ttctgagctg gcctctcact
     6421 gcctccccaa ggccccctgc ctgccctgtc aggaggcaga aggaagcagg tgtgagggca
     6481 gtgcaaggag ggagcacaac ccccagctcc cgctccgggc tccgacttgt gcacaggcag
     6541 agcccagacc ctggaggaaa tcctaccttt gaattcaaga acatttgggg aatttggaaa
     6601 tctctttgcc cccaaacccc cattctgtcc tacctttaat caggtcctgc tcagcagtga
     6661 gagcagatga ggtgaaaagg ccaagaggtt tggctcctgc ccactgatag cccctctccc
     6721 cgcagtgttt gtgtgtcaag tggcaaagct gttcttcctg gtgaccctga ttatatccag
     6781 taacacatag actgtgcgca taggcctgct ttgtctcctc tatcctgggc ttttgttttg
     6841 ctttttagtt ttgcttttag tttttctgtc ccttttattt aacgcaccga ctagacacac
     6901 aaagcagttg aatttttata tatatatctg tatattgcac aattataaac tcattttgct
     6961 tgtggctcca cacacacaaa aaaagacctg ttaaaattat acctgttgct taattacaat
     7021 atttctgata accatagcat aggacaaggg aaaataaaaa aagaaaaaaa agaaaaaaaa
     7081 acgacaaatc tgtctgctgg tcacttcttc tgtccaagca gattcgtggt cttttcctcg
     7141 cttctttcaa gggctttcct gtgccaggtg aaggaggctc caggcagcac ccaggttttg
     7201 cactcttgtt tctcccgtgc ttgtgaaaga ggtcccaagg ttctgggtgc aggagcgctc
     7261 ccttgacctg ctgaagtccg gaacgtagtc ggcacagcct ggtcgccttc cacctctggg
     7321 agctggagtc cactggggtg gcctgactcc cccagtcccc ttcccgtgac ctggtcaggg
     7381 tgagcccatg tggagtcagc ctcgcaggcc tccctgccag tagggtccga gtgtgtttca
     7441 tccttcccac tctgtcgagc ctgggggctg gagcggagac gggaggcctg gcctgtctcg
     7501 gaacctgtga gctgcaccag gtagaacgcc agggacccca gaatcatgtg cgtcagtcca
     7561 aggggtcccc tccaggagta gtgaagactc cagaaatgtc cctttcttct cccccatcct
     7621 acgagtaatt gcatttgctt ttgtaattct taatgagcaa tatctgctag agagtttagc
     7681 tgtaacagtt ctttttgatc atcttttttt aataattaga aacaccaaaa aaatccagaa
     7741 acttgttctt ccaaagcaga gagcattata atcaccaggg ccaaaagctt ccctccctgc
     7801 tgtcattgct tcttctgagg cctgaatcca aaagaaaaac agccataggc cctttcagtg
     7861 gccgggctac ccgtgagccc ttcggaggac cagggctggg gcagcctctg ggcccacatc
     7921 cggggccagc tccggcgtgt gttcagtgtt agcagtgggt catgatgctc tttcccaccc
     7981 agcctgggat aggggcagag gaggcgagga ggccgttgcc gctgatgttt ggccgtgaac
     8041 aggtgggtgt ctgcgtgcgt ccacgtgcgt gttttctgac tgacatgaaa tcgacgcccg
     8101 agttagcctc acccggtgac ctctagccct gcccggatgg agcggggccc acccggttca
     8161 gtgtttctgg ggagctggac agtggagtgc aaaaggcttg cagaacttga agcctgctcc
     8221 ttcccttgct accacggcct cctttccgtt tgatttgtca ctgcttcaat caataacagc
     8281 cgctccagag tcagtagtca atgaatatat gaccaaatat caccaggact gttactcaat
     8341 gtgtgccgag cccttgccca tgctgggctc ccgtgtatct ggacactgta acgtgtgctg
     8401 tgtttgctcc ccttcccctt ccttctttgc cctttacttg tctttctggg gtttttctgt
     8461 ttgggtttgg tttggttttt atttctcctt ttgtgttcca aacatgaggt tctctctact
     8521 ggtcctctta actgtggtgt tgaggcttat atttgtgtaa tttttggtgg gtgaaaggaa
     8581 ttttgctaag taaatctctt ctgtgtttga actgaagtct gtattgtaac tatgtttaaa
     8641 gtaattgttc cagagacaaa tatttctaga cactttttct ttacaaacaa aagcattcgg
     8701 agggaggggg atggtgactg agatgagagg ggagagctga acagatgacc cctgcccaga
     8761 tcagccagaa gccacccaaa gcagtggagc ccaggagtcc cactccaagc cagcaagccg
     8821 aatagctgat gtgttgccac tttccaagtc actgcaaaac caggttttgt tccgcccagt
     8881 ggattcttgt tttgcttccc ctccccccga gattattacc accatcccgt gcttttaagg
     8941 aaaggcaaga ttgatgtttc cttgagggga gccaggaggg gatgtgtgtg tgcagagctg
     9001 aagagctggg gagaatgggg ctgggcccac ccaagcagga ggctgggacg ctctgctgtg
     9061 ggcacaggtc aggctaatgt tggcagatgc agctcttcct ggacaggcca ggtggtgggc
     9121 attctctctc caaggtgtgc cccgtgggca ttactgttta agacacttcc gtcacatccc
     9181 accccatcct ccagggctca acactgtgac atctctattc cccaccctcc ccttcccagg
     9241 gcaataaaat gaccatggag ggggcttgca ctctcttggc tgtcacccga tcgccagcaa
     9301 aacttagatg tgagaaaacc ccttcccatt ccatggcgaa aacatctcct tagaaaagcc
     9361 attaccctca ttaggcatgg ttttgggctc ccaaaacacc tgacagcccc tccctcctct
     9421 gagaggcgga gagtgctgac tgtagtgacc attgcatgcc gggtgcagca tctggaagag
     9481 ctaggcaggg tgtctgcccc ctcctgagtt gaagtcatgc tcccctgtgc cagcccagag
     9541 gccgagagct atggacagca ttgccagtaa cacaggccac cctgtgcaga agggagctgg
     9601 ctccagcctg gaaacctgtc tgaggttggg agaggtgcac ttggggcaca gggagaggcc
     9661 gggacacact tagctggaga tgtctctaaa agccctgtat cgtattcacc ttcagttttt
     9721 gtgttttggg acaattactt tagaaaataa gtaggtcgtt ttaaaaacaa aaattattga
     9781 ttgctttttt gtagtgttca gaaaaaaggt tctttgtgta tagccaaatg actgaaagca
     9841 ctgatatatt taaaaacaaa aggcaattta ttaaggaaat ttgtaccatt tcagtaaacc
     9901 tgtctgaatg tacctgtata cgtttcaaaa acaccccccc cccactgaat ccctgtaacc
     9961 tatttattat ataaagagtt tgccttataa atttacataa aaatgtccgt ttgtgtcttt
    10021 tgttgtaaaa atcaagtgat tttttcataa ggttctttta ctattggaaa agatgggcag
    10081 cacgcagttt tattttattt ttgtaagttt tttaatacat gtgaaagcaa agaatactca
    10141 gcatgccttt ctaagtgacg cgtttgcacc ttttgttggg aagtactgta tcctgtgctg
    10201 ttagcattct cgataaatct ctctgtgaaa gtga
//
