LOCUS       NM_001386137           10470 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens methyl-CpG binding protein 2 (MECP2), transcript
            variant 8, mRNA.
ACCESSION   NM_001386137 XM_006724819
VERSION     NM_001386137.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10470)
  AUTHORS   Tomasello,D.L., Barrasa,M.I., Mankus,D., Alarcon,K.I.,
            Lytton-Jean,A.K.R., Liu,X.S. and Jaenisch,R.
  TITLE     Mitochondrial dysfunction and increased reactive oxygen species
            production in MECP2 mutant astrocytes and their impact on neurons
  JOURNAL   Sci Rep 14 (1), 20565 (2024)
   PUBMED   39232000
  REMARK    GeneRIF: Mitochondrial dysfunction and increased reactive oxygen
            species production in MECP2 mutant astrocytes and their impact on
            neurons.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10470)
  AUTHORS   Fichou,Y., Nectoux,J., Bahi-Buisson,N., Rosas-Vargas,H., Girard,B.,
            Chelly,J. and Bienvenu,T.
  TITLE     The first missense mutation causing Rett syndrome specifically
            affecting the MeCP2_e1 isoform
  JOURNAL   Neurogenetics 10 (2), 127-133 (2009)
   PUBMED   19034540
  REMARK    GeneRIF: Data suggest the important involvement of the N-terminus
            in the function of MeCP2 protein, and provide further evidence for
            the major impact of a specific MeCP2e_1 deficiency in the
            development of intellectual processing.
REFERENCE   3  (bases 1 to 10470)
  AUTHORS   Harvey,C.G., Menon,S.D., Stachowiak,B., Noor,A., Proctor,A.,
            Mensah,A.K., Mnatzakanian,G.N., Alfred,S.E., Guo,R., Scherer,S.W.,
            Kennedy,J.L., Roberts,W., Srivastava,A.K., Minassian,B.A. and
            Vincent,J.B.
  TITLE     Sequence variants within exon 1 of MECP2 occur in females with
            mental retardation
  JOURNAL   Am J Med Genet B Neuropsychiatr Genet 144B (3), 355-360 (2007)
   PUBMED   17171659
  REMARK    GeneRIF: polymorphisms within exon 1 of MECP2 occur in females with
            mental retardation.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Erratum:[Am J Med Genet B Neuropsychiatr Genet. 2007 Oct
            5;144(7):969. Srivistava, Anand K [corrected to Srivastava, Anand
            K]]
REFERENCE   4  (bases 1 to 10470)
  AUTHORS   Francke,U.
  TITLE     Mechanisms of disease: neurogenetics of MeCP2 deficiency
  JOURNAL   Nat Clin Pract Neurol 2 (4), 212-221 (2006)
   PUBMED   16932552
  REMARK    GeneRIF: Rett syndrome is caused by heterozygosity for mutations in
            the X-linked gene MECP2, which encodes methyl-CpG binding protein
            2.
            Review article
REFERENCE   5  (bases 1 to 10470)
  AUTHORS   Mnatzakanian,G.N., Lohi,H., Munteanu,I., Alfred,S.E., Yamada,T.,
            MacLeod,P.J., Jones,J.R., Scherer,S.W., Schanen,N.C., Friez,M.J.,
            Vincent,J.B. and Minassian,B.A.
  TITLE     A previously unidentified MECP2 open reading frame defines a new
            protein isoform relevant to Rett syndrome
  JOURNAL   Nat Genet 36 (4), 339-341 (2004)
   PUBMED   15034579
  REMARK    GeneRIF: description of a previously unknown MeCP2 isoform
            Erratum:[Nat Genet. 2004 May;36(5):540]
REFERENCE   6  (bases 1 to 10470)
  AUTHORS   Reichwald,K., Thiesen,J., Wiehe,T., Weitzel,J., Poustka,W.A.,
            Rosenthal,A., Platzer,M., Stratling,W.H. and Kioschis,P.
  TITLE     Comparative sequence analysis of the MECP2-locus in human and mouse
            reveals new transcribed regions
  JOURNAL   Mamm Genome 11 (3), 182-190 (2000)
   PUBMED   10723722
REFERENCE   7  (bases 1 to 10470)
  AUTHORS   Kaur,S. and Christodoulou,J.
  TITLE     MECP2 Disorders
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301670
REFERENCE   8  (bases 1 to 10470)
  AUTHORS   Van Esch,H.
  TITLE     MECP2 Duplication Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301461
REFERENCE   9  (bases 1 to 10470)
  AUTHORS   Klein,C., Lohmann,K., Marras,C. and Munchau,A.
  TITLE     Hereditary Dystonia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301334
REFERENCE   10 (bases 1 to 10470)
  AUTHORS   Lewis,J.D., Meehan,R.R., Henzel,W.J., Maurer-Fogy,I., Jeppesen,P.,
            Klein,F. and Bird,A.
  TITLE     Purification, sequence, and cellular localization of a novel
            chromosomal protein that binds to methylated DNA
  JOURNAL   Cell 69 (6), 905-914 (1992)
   PUBMED   1606614
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC244097.3, AF030876.2 and
            U52112.2.
            
            On Aug 11, 2020 this sequence version replaced XM_006724819.3.
            
            Summary: DNA methylation is the major modification of eukaryotic
            genomes and plays an essential role in mammalian development. Human
            proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of
            nuclear proteins related by the presence in each of a methyl-CpG
            binding domain (MBD). Each of these proteins, with the exception of
            MBD3, is capable of binding specifically to methylated DNA. MECP2,
            MBD1 and MBD2 can also repress transcription from methylated gene
            promoters. In contrast to other MBD family members, MECP2 is
            X-linked and subject to X inactivation. MECP2 is dispensible in
            stem cells, but is essential for embryonic development. MECP2 gene
            mutations are the cause of most cases of Rett syndrome, a
            progressive neurologic developmental disorder and one of the most
            common causes of cognitive disability in females. Alternative
            splicing results in multiple transcript variants encoding different
            isoforms. [provided by RefSeq, Oct 2015].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038197.1765790.1,
                                           SRR14038195.311422.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-114               AC244097.3         29110-29223         c
            115-238             AC244097.3         23690-23813         c
            239-350             AF030876.2         31264-31375         c
            351-701             AF030876.2         23679-24029         c
            702-756             AF030876.2         22868-22922         c
            757-3633            AF030876.2         19882-22758         c
            3634-10470          U52112.2           174507-181343       c
FEATURES             Location/Qualifiers
     source          1..10470
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq28"
     gene            1..10470
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="methyl-CpG binding protein 2"
                     /db_xref="GeneID:4204"
                     /db_xref="HGNC:HGNC:6990"
                     /db_xref="MIM:300005"
     exon            1..114
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     exon            115..238
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     exon            239..350
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     exon            351..701
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     exon            702..756
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     exon            757..10470
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     CDS             885..1676
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="isoform 4 is encoded by transcript variant 8;
                     meCp-2 protein"
                     /codon_start=1
                     /product="methyl-CpG-binding protein 2 isoform 4"
                     /protein_id="NP_001373066.1"
                     /db_xref="GeneID:4204"
                     /db_xref="HGNC:HGNC:6990"
                     /db_xref="MIM:300005"
                     /translation="MPFQTSPGGKAEGGGATTSTQVMVIKRPGRKRKAEADPQAIPKK
                     RGRKPGSVVAAAAAEAKKKAVKESSIRSVQETVLPIKKRKTRETVSIEVKEVVKPLLV
                     STLGEKSGKGLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHHHHHSESPKAPVPLL
                     PPLPPPPPEPESSEDPTSPPEPQDLSSSVCKEEKMPRGGSLESDGCPKEPAKTQPAVA
                     TAATAAEKYKHRGEGERKDIVSSSMPRPNREEPVDSRTPVTERVS"
     regulatory      10448..10453
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="hexamer: GATAAA"
     polyA_site      10470
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="major polyA site"
ORIGIN      
        1 gcgcgcgctc cctcctctcg gagagagggc tgtggtaaaa gccgtccgga aaatggccgc
       61 cgccgccgcc gccgcgccga gcggaggagg aggaggaggc gaggaggaga gactgctcca
      121 taaaaataca gactcaccag ttcctgcttt gatgtgacat gtgactcccc agaatacacc
      181 ttgcttctgt agaccagctc caacaggatt ccatggtagc tgggatgtta gggctcagca
      241 ctgagctagc ctctccttgc tagaagctta cggtcgaagg tctccagcca tcagaagaag
      301 cacgtggagc gctgcgtccg tgttgtggtt attcatccag catgtgttga ggaagaaaag
      361 tcagaagacc aggacctcca gggcctcaag gacaaacccc tcaagtttaa aaaggtgaag
      421 aaagataaga aagaagagaa agagggcaag catgagcccg tgcagccatc agcccaccac
      481 tctgctgagc ccgcagaggc aggcaaagca gagacatcag aagggtcagg ctccgccccg
      541 gctgtgccgg aagcttctgc ctcccccaaa cagcggcgct ccatcatccg tgaccgggga
      601 cccatgtatg atgaccccac cctgcctgaa ggctggacac ggaagcttaa gcaaaggaaa
      661 tctggccgct ctgctgggaa gtatgatgtg tatttgatca atccccaggg aaaagccttt
      721 cgctctaaag tggagttgat tgcgtacttc gaaaagctcc aggaactggc agaggccggg
      781 gacgccccaa agggagcggc accacgagac ccaaggcggc cacgtcagag ggtgtgcagg
      841 tgaaaagggt cctggagaaa agtcctggga agctccttgt caagatgcct tttcaaactt
      901 cgccaggggg caaggctgag gggggtgggg ccaccacatc cacccaggtc atggtgatca
      961 aacgccccgg caggaagcga aaagctgagg ccgaccctca ggccattccc aagaaacggg
     1021 gccgaaagcc ggggagtgtg gtggcagccg ctgccgccga ggccaaaaag aaagccgtga
     1081 aggagtcttc tatccgatct gtgcaggaga ccgtactccc catcaagaag cgcaagaccc
     1141 gggagacggt cagcatcgag gtcaaggaag tggtgaagcc cctgctggtg tccaccctcg
     1201 gtgagaagag cgggaaagga ctgaagacct gtaagagccc tgggcggaaa agcaaggaga
     1261 gcagccccaa ggggcgcagc agcagcgcct cctcaccccc caagaaggag caccaccacc
     1321 atcaccacca ctcagagtcc ccaaaggccc ccgtgccact gctcccaccc ctgcccccac
     1381 ctccacctga gcccgagagc tccgaggacc ccaccagccc ccctgagccc caggacttga
     1441 gcagcagcgt ctgcaaagag gagaagatgc ccagaggagg ctcactggag agcgacggct
     1501 gccccaagga gccagctaag actcagcccg cggttgccac cgccgccacg gccgcagaaa
     1561 agtacaaaca ccgaggggag ggagagcgca aagacattgt ttcatcctcc atgccaaggc
     1621 caaacagaga ggagcctgtg gacagccgga cgcccgtgac cgagagagtt agctgacttt
     1681 acacggagcg gattgcaaag caaaccaaca agaataaagg cagctgttgt ctcttctcct
     1741 tatgggtagg gctctgacaa agcttcccga ttaactgaaa taaaaaatat ttttttttct
     1801 ttcagtaaac ttagagtttc gtggcttcag ggtgggagta gttggagcat tggggatgtt
     1861 tttcttaccg acaagcacag tcaggttgaa gacctaacca gggccagaag tagctttgca
     1921 cttttctaaa ctaggctcct tcaacaaggc ttgctgcaga tactactgac cagacaagct
     1981 gttgaccagg cacctcccct cccgcccaaa cctttccccc atgtggtcgt tagagacaga
     2041 gcgacagagc agttgagagg acactcccgt tttcggtgcc atcagtgccc cgtctacagc
     2101 tcccccagct ccccccacct cccccactcc caaccacgtt gggacaggga ggtgtgaggc
     2161 aggagagaca gttggattct ttagagaaga tggatatgac cagtggctat ggcctgtgcg
     2221 atcccacccg tggtggctca agtctggccc cacaccagcc ccaatccaaa actggcaagg
     2281 acgcttcaca ggacaggaaa gtggcacctg tctgctccag ctctggcatg gctaggaggg
     2341 gggagtccct tgaactactg ggtgtagact ggcctgaacc acaggagagg atggcccagg
     2401 gtgaggtggc atggtccatt ctcaagggac gtcctccaac gggtggcgct agaggccatg
     2461 gaggcagtag gacaaggtgc aggcaggctg gcctggggtc aggccgggca gagcacagcg
     2521 gggtgagagg gattcctaat cactcagagc agtctgtgac ttagtggaca ggggaggggg
     2581 caaaggggga ggagaagaaa atgttcttcc agttactttc caattctcct ttagggacag
     2641 cttagaatta tttgcactat tgagtcttca tgttcccact tcaaaacaaa cagatgctct
     2701 gagagcaaac tggcttgaat tggtgacatt tagtccctca agccaccaga tgtgacagtg
     2761 ttgagaacta cctggatttg tatatatacc tgcgcttgtt ttaaagtggg ctcagcacat
     2821 agggttccca cgaagctccg aaactctaag tgtttgctgc aattttataa ggacttcctg
     2881 attggtttct cttctcccct tccatttctg ccttttgttc atttcatcct ttcacttctt
     2941 tcccttcctc catcctcctc cttcctagtt catcccttct cttccaggca gccgcggtgc
     3001 ccaaccacac ttgtcggctc cagtccccag aactctgcct gccctttgtc ctcctgctgc
     3061 cagtaccagc cccaccctgt tttgagccct gaggaggcct tgggctctgc tgagtccgac
     3121 ctggcctgtc tgtgaagagc aagagagcag caaggtcttg ctctcctagg tagccccctc
     3181 ttccctggta agaaaaagca aaaggcattt cccaccctga acaacgagcc ttttcaccct
     3241 tctactctag agaagtggac tggaggagct gggcccgatt tggtagttga ggaaagcaca
     3301 gaggcctcct gtggcctgcc agtcatcgag tggcccaaca ggggctccat gccagccgac
     3361 cttgacctca ctcagaagtc cagagtctag cgtagtgcag cagggcagta gcggtaccaa
     3421 tgcagaactc ccaagacccg agctgggacc agtacctggg tccccagccc ttcctctgct
     3481 cccccttttc cctcggagtt cttcttgaat ggcaatgttt tgcttttgct cgatgcagac
     3541 agggggccag aacaccacac atttcactgt ctgtctggtc catagctgtg gtgtaggggc
     3601 ttagaggcat gggcttgctg tgggttttta attgatcagt tttcatgtgg gatcccatct
     3661 ttttaacctc tgttcaggaa gtccttatct agctgcatat cttcatcata ttggtatatc
     3721 cttttctgtg tttacagaga tgtctcttat atctaaatct gtccaactga gaagtacctt
     3781 atcaaagtag caaatgagac agcagtctta tgcttccaga aacacccaca ggcatgtccc
     3841 atgtgagctg ctgccatgaa ctgtcaagtg tgtgttgtct tgtgtatttc agttattgtc
     3901 cctggcttcc ttactatggt gtaatcatga aggagtgaaa catcatagaa actgtctagc
     3961 acttccttgc cagtctttag tgatcaggaa ccatagttga cagttccaat cagtagctta
     4021 agaaaaaacc gtgtttgtct cttctggaat ggttagaagt gagggagttt gccccgttct
     4081 gtttgtagag tctcatagtt ggactttcta gcatatatgt gtccatttcc ttatgctgta
     4141 aaagcaagtc ctgcaaccaa actcccatca gcccaatccc tgatccctga tcccttccac
     4201 ctgctctgct gatgaccccc ccagcttcac ttctgactct tccccaggaa gggaaggggg
     4261 gtcagaagag agggtgagtc ctccagaact cttcctccaa ggacagaagg ctcctgcccc
     4321 catagtggcc tcgaactcct ggcactacca aaggacactt atccacgaga gcgcagcatc
     4381 cgaccaggtt gtcactgaga agatgtttat tttggtcagt tgggttttta tgtattatac
     4441 ttagtcaaat gtaatgtggc ttctggaatc attgtccaga gctgcttccc cgtcacctgg
     4501 gcgtcatctg gtcctggtaa gaggagtgcg tggcccacca ggcccccctg tcacccatga
     4561 cagttcattc agggccgatg gggcagtcgt ggttgggaac acagcatttc aagcgtcact
     4621 ttatttcatt cgggccccac ctgcagctcc ctcaaagagg cagttgccca gcctctttcc
     4681 cttccagttt attccagagc tgccagtggg gcctgaggct ccttagggtt ttctctctat
     4741 ttcccccttt cttcctcatt ccctcgtctt tcccaaaggc atcacgagtc agtcgccttt
     4801 cagcaggcag ccttggcggt ttatcgccct ggcaggcagg ggccctgcag ctctcatgct
     4861 gcccctgcct tggggtcagg ttgacaggag gttggaggga aagccttaag ctgcaggatt
     4921 ctcaccagct gtgtccggcc cagttttggg gtgtgacctc aatttcaatt ttgtctgtac
     4981 ttgaacatta tgaagatggg ggcctctttc agtgaatttg tgaacagcag aattgaccga
     5041 cagctttcca gtacccatgg ggctaggtca ttaaggccac atccacagtc tcccccaccc
     5101 ttgttccagt tgttagttac tacctcctct cctgacaata ctgtatgtcg tcgagctccc
     5161 cccaggtcta cccctcccgg ccctgcctgc tggtgggctt gtcatagcca gtgggattgc
     5221 cggtcttgac agctcagtga gctggagata cttggtcaca gccaggcgct agcacagctc
     5281 ccttctgttg atgctgtatt cccatatcaa aagacacagg ggacacccag aaacgccaca
     5341 tcccccaatc catcagtgcc aaactagcca acggccccag cttctcagct cgctggatgg
     5401 cggaagctgc tactcgtgag cgccagtgcg ggtgcagaca atcttctgtt gggtggcatc
     5461 attccaggcc cgaagcatga acagtgcacc tgggacaggg agcagcccca aattgtcacc
     5521 tgcttctctg cccagctttt cattgctgtg acagtgatgg cgaaagaggg taataaccag
     5581 acacaaactg ccaagttggg tggagaaagg agtttcttta gctgacagaa tctctgaatt
     5641 ttaaatcact tagtaagcgg ctcaagccca ggagggagca gagggatacg agcggagtcc
     5701 cctgcgcggg accatctgga attggtttag cccaagtgga gcctgacagc cagaactctg
     5761 tgtcccccgt ctaaccacag ctccttttcc agagcattcc agtcaggctc tctgggctga
     5821 ctgggccagg ggaggttaca ggtaccagtt ctttaagaag atctttgggc atatacattt
     5881 ttagcctgtg tcattgcccc aaatggattc ctgtttcaag ttcacacctg cagattctag
     5941 gacctgtgtc ctagacttca gggagtcagc tgtttctaga gttcctacca tggagtgggt
     6001 ctggaggacc tgcccggtgg gggggcagag ccctgctccc tccgggtctt cctactcttc
     6061 tctctgctct gacgggattt gttgattctc tccattttgg tgtctttctc ttttagatat
     6121 tgtatcaatc tttagaaaag gcatagtcta cttgttataa atcgttagga tactgcctcc
     6181 cccagggtct aaaattacat attagagggg aaaagctgaa cactgaagtc agttctcaac
     6241 aatttagaag gaaaacctag aaaacatttg gcagaaaatt acatttcgat gtttttgaat
     6301 gaatacgagc aagcttttac aacagtgctg atctaaaaat acttagcact tggcctgaga
     6361 tgcctggtga gcattacagg caaggggaat ctggaggtag ccgacctgag gacatggctt
     6421 ctgaacctgt cttttgggag tggtatggaa ggtggagcgt tcaccagtga cctggaaggc
     6481 ccagcaccac cctccttccc actcttctca tcttgacaga gcctgcccca gcgctgacgt
     6541 gtcaggaaaa cacccaggga actaggaagg cacttctgcc tgaggggcag cctgccttgc
     6601 ccactcctgc tctgctcgcc tcggatcagc tgagccttct gagctggcct ctcactgcct
     6661 ccccaaggcc ccctgcctgc cctgtcagga ggcagaagga agcaggtgtg agggcagtgc
     6721 aaggagggag cacaaccccc agctcccgct ccgggctccg acttgtgcac aggcagagcc
     6781 cagaccctgg aggaaatcct acctttgaat tcaagaacat ttggggaatt tggaaatctc
     6841 tttgccccca aacccccatt ctgtcctacc tttaatcagg tcctgctcag cagtgagagc
     6901 agatgaggtg aaaaggccaa gaggtttggc tcctgcccac tgatagcccc tctccccgca
     6961 gtgtttgtgt gtcaagtggc aaagctgttc ttcctggtga ccctgattat atccagtaac
     7021 acatagactg tgcgcatagg cctgctttgt ctcctctatc ctgggctttt gttttgcttt
     7081 ttagttttgc ttttagtttt tctgtccctt ttatttaacg caccgactag acacacaaag
     7141 cagttgaatt tttatatata tatctgtata ttgcacaatt ataaactcat tttgcttgtg
     7201 gctccacaca cacaaaaaaa gacctgttaa aattatacct gttgcttaat tacaatattt
     7261 ctgataacca tagcatagga caagggaaaa taaaaaaaga aaaaaaagaa aaaaaaacga
     7321 caaatctgtc tgctggtcac ttcttctgtc caagcagatt cgtggtcttt tcctcgcttc
     7381 tttcaagggc tttcctgtgc caggtgaagg aggctccagg cagcacccag gttttgcact
     7441 cttgtttctc ccgtgcttgt gaaagaggtc ccaaggttct gggtgcagga gcgctccctt
     7501 gacctgctga agtccggaac gtagtcggca cagcctggtc gccttccacc tctgggagct
     7561 ggagtccact ggggtggcct gactccccca gtccccttcc cgtgacctgg tcagggtgag
     7621 cccatgtgga gtcagcctcg caggcctccc tgccagtagg gtccgagtgt gtttcatcct
     7681 tcccactctg tcgagcctgg gggctggagc ggagacggga ggcctggcct gtctcggaac
     7741 ctgtgagctg caccaggtag aacgccaggg accccagaat catgtgcgtc agtccaaggg
     7801 gtcccctcca ggagtagtga agactccaga aatgtccctt tcttctcccc catcctacga
     7861 gtaattgcat ttgcttttgt aattcttaat gagcaatatc tgctagagag tttagctgta
     7921 acagttcttt ttgatcatct ttttttaata attagaaaca ccaaaaaaat ccagaaactt
     7981 gttcttccaa agcagagagc attataatca ccagggccaa aagcttccct ccctgctgtc
     8041 attgcttctt ctgaggcctg aatccaaaag aaaaacagcc ataggccctt tcagtggccg
     8101 ggctacccgt gagcccttcg gaggaccagg gctggggcag cctctgggcc cacatccggg
     8161 gccagctccg gcgtgtgttc agtgttagca gtgggtcatg atgctctttc ccacccagcc
     8221 tgggataggg gcagaggagg cgaggaggcc gttgccgctg atgtttggcc gtgaacaggt
     8281 gggtgtctgc gtgcgtccac gtgcgtgttt tctgactgac atgaaatcga cgcccgagtt
     8341 agcctcaccc ggtgacctct agccctgccc ggatggagcg gggcccaccc ggttcagtgt
     8401 ttctggggag ctggacagtg gagtgcaaaa ggcttgcaga acttgaagcc tgctccttcc
     8461 cttgctacca cggcctcctt tccgtttgat ttgtcactgc ttcaatcaat aacagccgct
     8521 ccagagtcag tagtcaatga atatatgacc aaatatcacc aggactgtta ctcaatgtgt
     8581 gccgagccct tgcccatgct gggctcccgt gtatctggac actgtaacgt gtgctgtgtt
     8641 tgctcccctt ccccttcctt ctttgccctt tacttgtctt tctggggttt ttctgtttgg
     8701 gtttggtttg gtttttattt ctccttttgt gttccaaaca tgaggttctc tctactggtc
     8761 ctcttaactg tggtgttgag gcttatattt gtgtaatttt tggtgggtga aaggaatttt
     8821 gctaagtaaa tctcttctgt gtttgaactg aagtctgtat tgtaactatg tttaaagtaa
     8881 ttgttccaga gacaaatatt tctagacact ttttctttac aaacaaaagc attcggaggg
     8941 agggggatgg tgactgagat gagaggggag agctgaacag atgacccctg cccagatcag
     9001 ccagaagcca cccaaagcag tggagcccag gagtcccact ccaagccagc aagccgaata
     9061 gctgatgtgt tgccactttc caagtcactg caaaaccagg ttttgttccg cccagtggat
     9121 tcttgttttg cttcccctcc ccccgagatt attaccacca tcccgtgctt ttaaggaaag
     9181 gcaagattga tgtttccttg aggggagcca ggaggggatg tgtgtgtgca gagctgaaga
     9241 gctggggaga atggggctgg gcccacccaa gcaggaggct gggacgctct gctgtgggca
     9301 caggtcaggc taatgttggc agatgcagct cttcctggac aggccaggtg gtgggcattc
     9361 tctctccaag gtgtgccccg tgggcattac tgtttaagac acttccgtca catcccaccc
     9421 catcctccag ggctcaacac tgtgacatct ctattcccca ccctcccctt cccagggcaa
     9481 taaaatgacc atggaggggg cttgcactct cttggctgtc acccgatcgc cagcaaaact
     9541 tagatgtgag aaaacccctt cccattccat ggcgaaaaca tctccttaga aaagccatta
     9601 ccctcattag gcatggtttt gggctcccaa aacacctgac agcccctccc tcctctgaga
     9661 ggcggagagt gctgactgta gtgaccattg catgccgggt gcagcatctg gaagagctag
     9721 gcagggtgtc tgccccctcc tgagttgaag tcatgctccc ctgtgccagc ccagaggccg
     9781 agagctatgg acagcattgc cagtaacaca ggccaccctg tgcagaaggg agctggctcc
     9841 agcctggaaa cctgtctgag gttgggagag gtgcacttgg ggcacaggga gaggccggga
     9901 cacacttagc tggagatgtc tctaaaagcc ctgtatcgta ttcaccttca gtttttgtgt
     9961 tttgggacaa ttactttaga aaataagtag gtcgttttaa aaacaaaaat tattgattgc
    10021 ttttttgtag tgttcagaaa aaaggttctt tgtgtatagc caaatgactg aaagcactga
    10081 tatatttaaa aacaaaaggc aatttattaa ggaaatttgt accatttcag taaacctgtc
    10141 tgaatgtacc tgtatacgtt tcaaaaacac ccccccccca ctgaatccct gtaacctatt
    10201 tattatataa agagtttgcc ttataaattt acataaaaat gtccgtttgt gtcttttgtt
    10261 gtaaaaatca agtgattttt tcataaggtt cttttactat tggaaaagat gggcagcacg
    10321 cagttttatt ttatttttgt aagtttttta atacatgtga aagcaaagaa tactcagcat
    10381 gcctttctaa gtgacgcgtt tgcacctttt gttgggaagt actgtatcct gtgctgttag
    10441 cattctcgat aaatctctct gtgaaagtga
//
