LOCUS       NM_001369394           10386 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens methyl-CpG binding protein 2 (MECP2), transcript
            variant 7, mRNA.
ACCESSION   NM_001369394
VERSION     NM_001369394.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10386)
  AUTHORS   Tomasello,D.L., Barrasa,M.I., Mankus,D., Alarcon,K.I.,
            Lytton-Jean,A.K.R., Liu,X.S. and Jaenisch,R.
  TITLE     Mitochondrial dysfunction and increased reactive oxygen species
            production in MECP2 mutant astrocytes and their impact on neurons
  JOURNAL   Sci Rep 14 (1), 20565 (2024)
   PUBMED   39232000
  REMARK    GeneRIF: Mitochondrial dysfunction and increased reactive oxygen
            species production in MECP2 mutant astrocytes and their impact on
            neurons.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10386)
  AUTHORS   Fichou,Y., Nectoux,J., Bahi-Buisson,N., Rosas-Vargas,H., Girard,B.,
            Chelly,J. and Bienvenu,T.
  TITLE     The first missense mutation causing Rett syndrome specifically
            affecting the MeCP2_e1 isoform
  JOURNAL   Neurogenetics 10 (2), 127-133 (2009)
   PUBMED   19034540
  REMARK    GeneRIF: Data suggest the important involvement of the N-terminus
            in the function of MeCP2 protein, and provide further evidence for
            the major impact of a specific MeCP2e_1 deficiency in the
            development of intellectual processing.
REFERENCE   3  (bases 1 to 10386)
  AUTHORS   Harvey,C.G., Menon,S.D., Stachowiak,B., Noor,A., Proctor,A.,
            Mensah,A.K., Mnatzakanian,G.N., Alfred,S.E., Guo,R., Scherer,S.W.,
            Kennedy,J.L., Roberts,W., Srivastava,A.K., Minassian,B.A. and
            Vincent,J.B.
  TITLE     Sequence variants within exon 1 of MECP2 occur in females with
            mental retardation
  JOURNAL   Am J Med Genet B Neuropsychiatr Genet 144B (3), 355-360 (2007)
   PUBMED   17171659
  REMARK    GeneRIF: polymorphisms within exon 1 of MECP2 occur in females with
            mental retardation.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Erratum:[Am J Med Genet B Neuropsychiatr Genet. 2007 Oct
            5;144(7):969. Srivistava, Anand K [corrected to Srivastava, Anand
            K]]
REFERENCE   4  (bases 1 to 10386)
  AUTHORS   Francke,U.
  TITLE     Mechanisms of disease: neurogenetics of MeCP2 deficiency
  JOURNAL   Nat Clin Pract Neurol 2 (4), 212-221 (2006)
   PUBMED   16932552
  REMARK    GeneRIF: Rett syndrome is caused by heterozygosity for mutations in
            the X-linked gene MECP2, which encodes methyl-CpG binding protein
            2.
            Review article
REFERENCE   5  (bases 1 to 10386)
  AUTHORS   Mnatzakanian,G.N., Lohi,H., Munteanu,I., Alfred,S.E., Yamada,T.,
            MacLeod,P.J., Jones,J.R., Scherer,S.W., Schanen,N.C., Friez,M.J.,
            Vincent,J.B. and Minassian,B.A.
  TITLE     A previously unidentified MECP2 open reading frame defines a new
            protein isoform relevant to Rett syndrome
  JOURNAL   Nat Genet 36 (4), 339-341 (2004)
   PUBMED   15034579
  REMARK    GeneRIF: description of a previously unknown MeCP2 isoform
            Erratum:[Nat Genet. 2004 May;36(5):540]
REFERENCE   6  (bases 1 to 10386)
  AUTHORS   Reichwald,K., Thiesen,J., Wiehe,T., Weitzel,J., Poustka,W.A.,
            Rosenthal,A., Platzer,M., Stratling,W.H. and Kioschis,P.
  TITLE     Comparative sequence analysis of the MECP2-locus in human and mouse
            reveals new transcribed regions
  JOURNAL   Mamm Genome 11 (3), 182-190 (2000)
   PUBMED   10723722
REFERENCE   7  (bases 1 to 10386)
  AUTHORS   Kaur,S. and Christodoulou,J.
  TITLE     MECP2 Disorders
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301670
REFERENCE   8  (bases 1 to 10386)
  AUTHORS   Van Esch,H.
  TITLE     MECP2 Duplication Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301461
REFERENCE   9  (bases 1 to 10386)
  AUTHORS   Klein,C., Lohmann,K., Marras,C. and Munchau,A.
  TITLE     Hereditary Dystonia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301334
REFERENCE   10 (bases 1 to 10386)
  AUTHORS   Lewis,J.D., Meehan,R.R., Henzel,W.J., Maurer-Fogy,I., Jeppesen,P.,
            Klein,F. and Bird,A.
  TITLE     Purification, sequence, and cellular localization of a novel
            chromosomal protein that binds to methylated DNA
  JOURNAL   Cell 69 (6), 905-914 (1992)
   PUBMED   1606614
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC244097.3, AF030876.2 and
            U52112.2.
            
            On May 21, 2020 this sequence version replaced NM_001369394.1.
            
            Summary: DNA methylation is the major modification of eukaryotic
            genomes and plays an essential role in mammalian development. Human
            proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of
            nuclear proteins related by the presence in each of a methyl-CpG
            binding domain (MBD). Each of these proteins, with the exception of
            MBD3, is capable of binding specifically to methylated DNA. MECP2,
            MBD1 and MBD2 can also repress transcription from methylated gene
            promoters. In contrast to other MBD family members, MECP2 is
            X-linked and subject to X inactivation. MECP2 is dispensible in
            stem cells, but is essential for embryonic development. MECP2 gene
            mutations are the cause of most cases of Rett syndrome, a
            progressive neurologic developmental disorder and one of the most
            common causes of cognitive disability in females. Alternative
            splicing results in multiple transcript variants encoding different
            isoforms. [provided by RefSeq, Oct 2015].
            
            Transcript Variant: This variant (7) differs in the 5' UTR and
            coding sequence, compared to variant 1. The encoded isoform (3) has
            a shorter N-terminus compared to isoform 1. Variants 3-7 all encode
            the same isoform (3).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14372080.409046.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1968968,
                                           SAMEA2144333 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 5' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-157               AC244097.3         28319-28475         c
            158-508             AF030876.2         23679-24029         c
            509-3549            AF030876.2         19882-22922         c
            3550-10386          U52112.2           174507-181343       c
FEATURES             Location/Qualifiers
     source          1..10386
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq28"
     gene            1..10386
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="methyl-CpG binding protein 2"
                     /db_xref="GeneID:4204"
                     /db_xref="HGNC:HGNC:6990"
                     /db_xref="MIM:300005"
     exon            1..157
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     exon            158..508
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     CDS             411..1592
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="isoform 3 is encoded by transcript variant 7;
                     meCp-2 protein"
                     /codon_start=1
                     /product="methyl-CpG-binding protein 2 isoform 3"
                     /protein_id="NP_001356323.1"
                     /db_xref="GeneID:4204"
                     /db_xref="HGNC:HGNC:6990"
                     /db_xref="MIM:300005"
                     /translation="MYDDPTLPEGWTRKLKQRKSGRSAGKYDVYLINPQGKAFRSKVE
                     LIAYFEKVGDTSLDPNDFDFTVTGRGSPSRREQKPPKKPKSPKAPGTGRGRGRPKGSG
                     TTRPKAATSEGVQVKRVLEKSPGKLLVKMPFQTSPGGKAEGGGATTSTQVMVIKRPGR
                     KRKAEADPQAIPKKRGRKPGSVVAAAAAEAKKKAVKESSIRSVQETVLPIKKRKTRET
                     VSIEVKEVVKPLLVSTLGEKSGKGLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHH
                     HHHSESPKAPVPLLPPLPPPPPEPESSEDPTSPPEPQDLSSSVCKEEKMPRGGSLESD
                     GCPKEPAKTQPAVATAATAAEKYKHRGEGERKDIVSSSMPRPNREEPVDSRTPVTERV
                     S"
     exon            509..10386
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10364..10369
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="hexamer: GATAAA"
     polyA_site      10386
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="major polyA site"
ORIGIN      
        1 agtaggtggg gattattatc cacaaaaggg actagacatt gtgttctggg tcccacaact
       61 catcataaag aggtggttat agttcccatc aggagccgtg ggtaggggac tgtgcgtcca
      121 gcagcacccg aggctcttcg gcgccagagg ctctaaggga agaaaagtca gaagaccagg
      181 acctccaggg cctcaaggac aaacccctca agtttaaaaa ggtgaagaaa gataagaaag
      241 aagagaaaga gggcaagcat gagcccgtgc agccatcagc ccaccactct gctgagcccg
      301 cagaggcagg caaagcagag acatcagaag ggtcaggctc cgccccggct gtgccggaag
      361 cttctgcctc ccccaaacag cggcgctcca tcatccgtga ccggggaccc atgtatgatg
      421 accccaccct gcctgaaggc tggacacgga agcttaagca aaggaaatct ggccgctctg
      481 ctgggaagta tgatgtgtat ttgatcaatc cccagggaaa agcctttcgc tctaaagtgg
      541 agttgattgc gtacttcgaa aaggtaggcg acacatccct ggaccctaat gattttgact
      601 tcacggtaac tgggagaggg agcccctccc ggcgagagca gaaaccacct aagaagccca
      661 aatctcccaa agctccagga actggcagag gccggggacg ccccaaaggg agcggcacca
      721 cgagacccaa ggcggccacg tcagagggtg tgcaggtgaa aagggtcctg gagaaaagtc
      781 ctgggaagct ccttgtcaag atgccttttc aaacttcgcc agggggcaag gctgaggggg
      841 gtggggccac cacatccacc caggtcatgg tgatcaaacg ccccggcagg aagcgaaaag
      901 ctgaggccga ccctcaggcc attcccaaga aacggggccg aaagccgggg agtgtggtgg
      961 cagccgctgc cgccgaggcc aaaaagaaag ccgtgaagga gtcttctatc cgatctgtgc
     1021 aggagaccgt actccccatc aagaagcgca agacccggga gacggtcagc atcgaggtca
     1081 aggaagtggt gaagcccctg ctggtgtcca ccctcggtga gaagagcggg aaaggactga
     1141 agacctgtaa gagccctggg cggaaaagca aggagagcag ccccaagggg cgcagcagca
     1201 gcgcctcctc accccccaag aaggagcacc accaccatca ccaccactca gagtccccaa
     1261 aggcccccgt gccactgctc ccacccctgc ccccacctcc acctgagccc gagagctccg
     1321 aggaccccac cagcccccct gagccccagg acttgagcag cagcgtctgc aaagaggaga
     1381 agatgcccag aggaggctca ctggagagcg acggctgccc caaggagcca gctaagactc
     1441 agcccgcggt tgccaccgcc gccacggccg cagaaaagta caaacaccga ggggagggag
     1501 agcgcaaaga cattgtttca tcctccatgc caaggccaaa cagagaggag cctgtggaca
     1561 gccggacgcc cgtgaccgag agagttagct gactttacac ggagcggatt gcaaagcaaa
     1621 ccaacaagaa taaaggcagc tgttgtctct tctccttatg ggtagggctc tgacaaagct
     1681 tcccgattaa ctgaaataaa aaatattttt ttttctttca gtaaacttag agtttcgtgg
     1741 cttcagggtg ggagtagttg gagcattggg gatgtttttc ttaccgacaa gcacagtcag
     1801 gttgaagacc taaccagggc cagaagtagc tttgcacttt tctaaactag gctccttcaa
     1861 caaggcttgc tgcagatact actgaccaga caagctgttg accaggcacc tcccctcccg
     1921 cccaaacctt tcccccatgt ggtcgttaga gacagagcga cagagcagtt gagaggacac
     1981 tcccgttttc ggtgccatca gtgccccgtc tacagctccc ccagctcccc ccacctcccc
     2041 cactcccaac cacgttggga cagggaggtg tgaggcagga gagacagttg gattctttag
     2101 agaagatgga tatgaccagt ggctatggcc tgtgcgatcc cacccgtggt ggctcaagtc
     2161 tggccccaca ccagccccaa tccaaaactg gcaaggacgc ttcacaggac aggaaagtgg
     2221 cacctgtctg ctccagctct ggcatggcta ggagggggga gtcccttgaa ctactgggtg
     2281 tagactggcc tgaaccacag gagaggatgg cccagggtga ggtggcatgg tccattctca
     2341 agggacgtcc tccaacgggt ggcgctagag gccatggagg cagtaggaca aggtgcaggc
     2401 aggctggcct ggggtcaggc cgggcagagc acagcggggt gagagggatt cctaatcact
     2461 cagagcagtc tgtgacttag tggacagggg agggggcaaa gggggaggag aagaaaatgt
     2521 tcttccagtt actttccaat tctcctttag ggacagctta gaattatttg cactattgag
     2581 tcttcatgtt cccacttcaa aacaaacaga tgctctgaga gcaaactggc ttgaattggt
     2641 gacatttagt ccctcaagcc accagatgtg acagtgttga gaactacctg gatttgtata
     2701 tatacctgcg cttgttttaa agtgggctca gcacataggg ttcccacgaa gctccgaaac
     2761 tctaagtgtt tgctgcaatt ttataaggac ttcctgattg gtttctcttc tccccttcca
     2821 tttctgcctt ttgttcattt catcctttca cttctttccc ttcctccatc ctcctccttc
     2881 ctagttcatc ccttctcttc caggcagccg cggtgcccaa ccacacttgt cggctccagt
     2941 ccccagaact ctgcctgccc tttgtcctcc tgctgccagt accagcccca ccctgttttg
     3001 agccctgagg aggccttggg ctctgctgag tccgacctgg cctgtctgtg aagagcaaga
     3061 gagcagcaag gtcttgctct cctaggtagc cccctcttcc ctggtaagaa aaagcaaaag
     3121 gcatttccca ccctgaacaa cgagcctttt cacccttcta ctctagagaa gtggactgga
     3181 ggagctgggc ccgatttggt agttgaggaa agcacagagg cctcctgtgg cctgccagtc
     3241 atcgagtggc ccaacagggg ctccatgcca gccgaccttg acctcactca gaagtccaga
     3301 gtctagcgta gtgcagcagg gcagtagcgg taccaatgca gaactcccaa gacccgagct
     3361 gggaccagta cctgggtccc cagcccttcc tctgctcccc cttttccctc ggagttcttc
     3421 ttgaatggca atgttttgct tttgctcgat gcagacaggg ggccagaaca ccacacattt
     3481 cactgtctgt ctggtccata gctgtggtgt aggggcttag aggcatgggc ttgctgtggg
     3541 tttttaattg atcagttttc atgtgggatc ccatcttttt aacctctgtt caggaagtcc
     3601 ttatctagct gcatatcttc atcatattgg tatatccttt tctgtgttta cagagatgtc
     3661 tcttatatct aaatctgtcc aactgagaag taccttatca aagtagcaaa tgagacagca
     3721 gtcttatgct tccagaaaca cccacaggca tgtcccatgt gagctgctgc catgaactgt
     3781 caagtgtgtg ttgtcttgtg tatttcagtt attgtccctg gcttccttac tatggtgtaa
     3841 tcatgaagga gtgaaacatc atagaaactg tctagcactt ccttgccagt ctttagtgat
     3901 caggaaccat agttgacagt tccaatcagt agcttaagaa aaaaccgtgt ttgtctcttc
     3961 tggaatggtt agaagtgagg gagtttgccc cgttctgttt gtagagtctc atagttggac
     4021 tttctagcat atatgtgtcc atttccttat gctgtaaaag caagtcctgc aaccaaactc
     4081 ccatcagccc aatccctgat ccctgatccc ttccacctgc tctgctgatg acccccccag
     4141 cttcacttct gactcttccc caggaaggga aggggggtca gaagagaggg tgagtcctcc
     4201 agaactcttc ctccaaggac agaaggctcc tgcccccata gtggcctcga actcctggca
     4261 ctaccaaagg acacttatcc acgagagcgc agcatccgac caggttgtca ctgagaagat
     4321 gtttattttg gtcagttggg tttttatgta ttatacttag tcaaatgtaa tgtggcttct
     4381 ggaatcattg tccagagctg cttccccgtc acctgggcgt catctggtcc tggtaagagg
     4441 agtgcgtggc ccaccaggcc cccctgtcac ccatgacagt tcattcaggg ccgatggggc
     4501 agtcgtggtt gggaacacag catttcaagc gtcactttat ttcattcggg ccccacctgc
     4561 agctccctca aagaggcagt tgcccagcct ctttcccttc cagtttattc cagagctgcc
     4621 agtggggcct gaggctcctt agggttttct ctctatttcc ccctttcttc ctcattccct
     4681 cgtctttccc aaaggcatca cgagtcagtc gcctttcagc aggcagcctt ggcggtttat
     4741 cgccctggca ggcaggggcc ctgcagctct catgctgccc ctgccttggg gtcaggttga
     4801 caggaggttg gagggaaagc cttaagctgc aggattctca ccagctgtgt ccggcccagt
     4861 tttggggtgt gacctcaatt tcaattttgt ctgtacttga acattatgaa gatgggggcc
     4921 tctttcagtg aatttgtgaa cagcagaatt gaccgacagc tttccagtac ccatggggct
     4981 aggtcattaa ggccacatcc acagtctccc ccacccttgt tccagttgtt agttactacc
     5041 tcctctcctg acaatactgt atgtcgtcga gctcccccca ggtctacccc tcccggccct
     5101 gcctgctggt gggcttgtca tagccagtgg gattgccggt cttgacagct cagtgagctg
     5161 gagatacttg gtcacagcca ggcgctagca cagctccctt ctgttgatgc tgtattccca
     5221 tatcaaaaga cacaggggac acccagaaac gccacatccc ccaatccatc agtgccaaac
     5281 tagccaacgg ccccagcttc tcagctcgct ggatggcgga agctgctact cgtgagcgcc
     5341 agtgcgggtg cagacaatct tctgttgggt ggcatcattc caggcccgaa gcatgaacag
     5401 tgcacctggg acagggagca gccccaaatt gtcacctgct tctctgccca gcttttcatt
     5461 gctgtgacag tgatggcgaa agagggtaat aaccagacac aaactgccaa gttgggtgga
     5521 gaaaggagtt tctttagctg acagaatctc tgaattttaa atcacttagt aagcggctca
     5581 agcccaggag ggagcagagg gatacgagcg gagtcccctg cgcgggacca tctggaattg
     5641 gtttagccca agtggagcct gacagccaga actctgtgtc ccccgtctaa ccacagctcc
     5701 ttttccagag cattccagtc aggctctctg ggctgactgg gccaggggag gttacaggta
     5761 ccagttcttt aagaagatct ttgggcatat acatttttag cctgtgtcat tgccccaaat
     5821 ggattcctgt ttcaagttca cacctgcaga ttctaggacc tgtgtcctag acttcaggga
     5881 gtcagctgtt tctagagttc ctaccatgga gtgggtctgg aggacctgcc cggtgggggg
     5941 gcagagccct gctccctccg ggtcttccta ctcttctctc tgctctgacg ggatttgttg
     6001 attctctcca ttttggtgtc tttctctttt agatattgta tcaatcttta gaaaaggcat
     6061 agtctacttg ttataaatcg ttaggatact gcctccccca gggtctaaaa ttacatatta
     6121 gaggggaaaa gctgaacact gaagtcagtt ctcaacaatt tagaaggaaa acctagaaaa
     6181 catttggcag aaaattacat ttcgatgttt ttgaatgaat acgagcaagc ttttacaaca
     6241 gtgctgatct aaaaatactt agcacttggc ctgagatgcc tggtgagcat tacaggcaag
     6301 gggaatctgg aggtagccga cctgaggaca tggcttctga acctgtcttt tgggagtggt
     6361 atggaaggtg gagcgttcac cagtgacctg gaaggcccag caccaccctc cttcccactc
     6421 ttctcatctt gacagagcct gccccagcgc tgacgtgtca ggaaaacacc cagggaacta
     6481 ggaaggcact tctgcctgag gggcagcctg ccttgcccac tcctgctctg ctcgcctcgg
     6541 atcagctgag ccttctgagc tggcctctca ctgcctcccc aaggccccct gcctgccctg
     6601 tcaggaggca gaaggaagca ggtgtgaggg cagtgcaagg agggagcaca acccccagct
     6661 cccgctccgg gctccgactt gtgcacaggc agagcccaga ccctggagga aatcctacct
     6721 ttgaattcaa gaacatttgg ggaatttgga aatctctttg cccccaaacc cccattctgt
     6781 cctaccttta atcaggtcct gctcagcagt gagagcagat gaggtgaaaa ggccaagagg
     6841 tttggctcct gcccactgat agcccctctc cccgcagtgt ttgtgtgtca agtggcaaag
     6901 ctgttcttcc tggtgaccct gattatatcc agtaacacat agactgtgcg cataggcctg
     6961 ctttgtctcc tctatcctgg gcttttgttt tgctttttag ttttgctttt agtttttctg
     7021 tcccttttat ttaacgcacc gactagacac acaaagcagt tgaattttta tatatatatc
     7081 tgtatattgc acaattataa actcattttg cttgtggctc cacacacaca aaaaaagacc
     7141 tgttaaaatt atacctgttg cttaattaca atatttctga taaccatagc ataggacaag
     7201 ggaaaataaa aaaagaaaaa aaagaaaaaa aaacgacaaa tctgtctgct ggtcacttct
     7261 tctgtccaag cagattcgtg gtcttttcct cgcttctttc aagggctttc ctgtgccagg
     7321 tgaaggaggc tccaggcagc acccaggttt tgcactcttg tttctcccgt gcttgtgaaa
     7381 gaggtcccaa ggttctgggt gcaggagcgc tcccttgacc tgctgaagtc cggaacgtag
     7441 tcggcacagc ctggtcgcct tccacctctg ggagctggag tccactgggg tggcctgact
     7501 cccccagtcc ccttcccgtg acctggtcag ggtgagccca tgtggagtca gcctcgcagg
     7561 cctccctgcc agtagggtcc gagtgtgttt catccttccc actctgtcga gcctgggggc
     7621 tggagcggag acgggaggcc tggcctgtct cggaacctgt gagctgcacc aggtagaacg
     7681 ccagggaccc cagaatcatg tgcgtcagtc caaggggtcc cctccaggag tagtgaagac
     7741 tccagaaatg tccctttctt ctcccccatc ctacgagtaa ttgcatttgc ttttgtaatt
     7801 cttaatgagc aatatctgct agagagttta gctgtaacag ttctttttga tcatcttttt
     7861 ttaataatta gaaacaccaa aaaaatccag aaacttgttc ttccaaagca gagagcatta
     7921 taatcaccag ggccaaaagc ttccctccct gctgtcattg cttcttctga ggcctgaatc
     7981 caaaagaaaa acagccatag gccctttcag tggccgggct acccgtgagc ccttcggagg
     8041 accagggctg gggcagcctc tgggcccaca tccggggcca gctccggcgt gtgttcagtg
     8101 ttagcagtgg gtcatgatgc tctttcccac ccagcctggg ataggggcag aggaggcgag
     8161 gaggccgttg ccgctgatgt ttggccgtga acaggtgggt gtctgcgtgc gtccacgtgc
     8221 gtgttttctg actgacatga aatcgacgcc cgagttagcc tcacccggtg acctctagcc
     8281 ctgcccggat ggagcggggc ccacccggtt cagtgtttct ggggagctgg acagtggagt
     8341 gcaaaaggct tgcagaactt gaagcctgct ccttcccttg ctaccacggc ctcctttccg
     8401 tttgatttgt cactgcttca atcaataaca gccgctccag agtcagtagt caatgaatat
     8461 atgaccaaat atcaccagga ctgttactca atgtgtgccg agcccttgcc catgctgggc
     8521 tcccgtgtat ctggacactg taacgtgtgc tgtgtttgct ccccttcccc ttccttcttt
     8581 gccctttact tgtctttctg gggtttttct gtttgggttt ggtttggttt ttatttctcc
     8641 ttttgtgttc caaacatgag gttctctcta ctggtcctct taactgtggt gttgaggctt
     8701 atatttgtgt aatttttggt gggtgaaagg aattttgcta agtaaatctc ttctgtgttt
     8761 gaactgaagt ctgtattgta actatgttta aagtaattgt tccagagaca aatatttcta
     8821 gacacttttt ctttacaaac aaaagcattc ggagggaggg ggatggtgac tgagatgaga
     8881 ggggagagct gaacagatga cccctgccca gatcagccag aagccaccca aagcagtgga
     8941 gcccaggagt cccactccaa gccagcaagc cgaatagctg atgtgttgcc actttccaag
     9001 tcactgcaaa accaggtttt gttccgccca gtggattctt gttttgcttc ccctcccccc
     9061 gagattatta ccaccatccc gtgcttttaa ggaaaggcaa gattgatgtt tccttgaggg
     9121 gagccaggag gggatgtgtg tgtgcagagc tgaagagctg gggagaatgg ggctgggccc
     9181 acccaagcag gaggctggga cgctctgctg tgggcacagg tcaggctaat gttggcagat
     9241 gcagctcttc ctggacaggc caggtggtgg gcattctctc tccaaggtgt gccccgtggg
     9301 cattactgtt taagacactt ccgtcacatc ccaccccatc ctccagggct caacactgtg
     9361 acatctctat tccccaccct ccccttccca gggcaataaa atgaccatgg agggggcttg
     9421 cactctcttg gctgtcaccc gatcgccagc aaaacttaga tgtgagaaaa ccccttccca
     9481 ttccatggcg aaaacatctc cttagaaaag ccattaccct cattaggcat ggttttgggc
     9541 tcccaaaaca cctgacagcc cctccctcct ctgagaggcg gagagtgctg actgtagtga
     9601 ccattgcatg ccgggtgcag catctggaag agctaggcag ggtgtctgcc ccctcctgag
     9661 ttgaagtcat gctcccctgt gccagcccag aggccgagag ctatggacag cattgccagt
     9721 aacacaggcc accctgtgca gaagggagct ggctccagcc tggaaacctg tctgaggttg
     9781 ggagaggtgc acttggggca cagggagagg ccgggacaca cttagctgga gatgtctcta
     9841 aaagccctgt atcgtattca ccttcagttt ttgtgttttg ggacaattac tttagaaaat
     9901 aagtaggtcg ttttaaaaac aaaaattatt gattgctttt ttgtagtgtt cagaaaaaag
     9961 gttctttgtg tatagccaaa tgactgaaag cactgatata tttaaaaaca aaaggcaatt
    10021 tattaaggaa atttgtacca tttcagtaaa cctgtctgaa tgtacctgta tacgtttcaa
    10081 aaacaccccc cccccactga atccctgtaa cctatttatt atataaagag tttgccttat
    10141 aaatttacat aaaaatgtcc gtttgtgtct tttgttgtaa aaatcaagtg attttttcat
    10201 aaggttcttt tactattgga aaagatgggc agcacgcagt tttattttat ttttgtaagt
    10261 tttttaatac atgtgaaagc aaagaatact cagcatgcct ttctaagtga cgcgtttgca
    10321 ccttttgttg ggaagtactg tatcctgtgc tgttagcatt ctcgataaat ctctctgtga
    10381 aagtga
//
