LOCUS       NM_001369393           10455 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens methyl-CpG binding protein 2 (MECP2), transcript
            variant 6, mRNA.
ACCESSION   NM_001369393
VERSION     NM_001369393.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10455)
  AUTHORS   Tomasello,D.L., Barrasa,M.I., Mankus,D., Alarcon,K.I.,
            Lytton-Jean,A.K.R., Liu,X.S. and Jaenisch,R.
  TITLE     Mitochondrial dysfunction and increased reactive oxygen species
            production in MECP2 mutant astrocytes and their impact on neurons
  JOURNAL   Sci Rep 14 (1), 20565 (2024)
   PUBMED   39232000
  REMARK    GeneRIF: Mitochondrial dysfunction and increased reactive oxygen
            species production in MECP2 mutant astrocytes and their impact on
            neurons.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10455)
  AUTHORS   Fichou,Y., Nectoux,J., Bahi-Buisson,N., Rosas-Vargas,H., Girard,B.,
            Chelly,J. and Bienvenu,T.
  TITLE     The first missense mutation causing Rett syndrome specifically
            affecting the MeCP2_e1 isoform
  JOURNAL   Neurogenetics 10 (2), 127-133 (2009)
   PUBMED   19034540
  REMARK    GeneRIF: Data suggest the important involvement of the N-terminus
            in the function of MeCP2 protein, and provide further evidence for
            the major impact of a specific MeCP2e_1 deficiency in the
            development of intellectual processing.
REFERENCE   3  (bases 1 to 10455)
  AUTHORS   Harvey,C.G., Menon,S.D., Stachowiak,B., Noor,A., Proctor,A.,
            Mensah,A.K., Mnatzakanian,G.N., Alfred,S.E., Guo,R., Scherer,S.W.,
            Kennedy,J.L., Roberts,W., Srivastava,A.K., Minassian,B.A. and
            Vincent,J.B.
  TITLE     Sequence variants within exon 1 of MECP2 occur in females with
            mental retardation
  JOURNAL   Am J Med Genet B Neuropsychiatr Genet 144B (3), 355-360 (2007)
   PUBMED   17171659
  REMARK    GeneRIF: polymorphisms within exon 1 of MECP2 occur in females with
            mental retardation.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Erratum:[Am J Med Genet B Neuropsychiatr Genet. 2007 Oct
            5;144(7):969. Srivistava, Anand K [corrected to Srivastava, Anand
            K]]
REFERENCE   4  (bases 1 to 10455)
  AUTHORS   Francke,U.
  TITLE     Mechanisms of disease: neurogenetics of MeCP2 deficiency
  JOURNAL   Nat Clin Pract Neurol 2 (4), 212-221 (2006)
   PUBMED   16932552
  REMARK    GeneRIF: Rett syndrome is caused by heterozygosity for mutations in
            the X-linked gene MECP2, which encodes methyl-CpG binding protein
            2.
            Review article
REFERENCE   5  (bases 1 to 10455)
  AUTHORS   Mnatzakanian,G.N., Lohi,H., Munteanu,I., Alfred,S.E., Yamada,T.,
            MacLeod,P.J., Jones,J.R., Scherer,S.W., Schanen,N.C., Friez,M.J.,
            Vincent,J.B. and Minassian,B.A.
  TITLE     A previously unidentified MECP2 open reading frame defines a new
            protein isoform relevant to Rett syndrome
  JOURNAL   Nat Genet 36 (4), 339-341 (2004)
   PUBMED   15034579
  REMARK    GeneRIF: description of a previously unknown MeCP2 isoform
            Erratum:[Nat Genet. 2004 May;36(5):540]
REFERENCE   6  (bases 1 to 10455)
  AUTHORS   Reichwald,K., Thiesen,J., Wiehe,T., Weitzel,J., Poustka,W.A.,
            Rosenthal,A., Platzer,M., Stratling,W.H. and Kioschis,P.
  TITLE     Comparative sequence analysis of the MECP2-locus in human and mouse
            reveals new transcribed regions
  JOURNAL   Mamm Genome 11 (3), 182-190 (2000)
   PUBMED   10723722
REFERENCE   7  (bases 1 to 10455)
  AUTHORS   Kaur,S. and Christodoulou,J.
  TITLE     MECP2 Disorders
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301670
REFERENCE   8  (bases 1 to 10455)
  AUTHORS   Van Esch,H.
  TITLE     MECP2 Duplication Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301461
REFERENCE   9  (bases 1 to 10455)
  AUTHORS   Klein,C., Lohmann,K., Marras,C. and Munchau,A.
  TITLE     Hereditary Dystonia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301334
REFERENCE   10 (bases 1 to 10455)
  AUTHORS   Lewis,J.D., Meehan,R.R., Henzel,W.J., Maurer-Fogy,I., Jeppesen,P.,
            Klein,F. and Bird,A.
  TITLE     Purification, sequence, and cellular localization of a novel
            chromosomal protein that binds to methylated DNA
  JOURNAL   Cell 69 (6), 905-914 (1992)
   PUBMED   1606614
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC244097.3, AF030876.2 and
            U52112.2.
            
            On Jun 2, 2019 this sequence version replaced NM_001369393.1.
            
            Summary: DNA methylation is the major modification of eukaryotic
            genomes and plays an essential role in mammalian development. Human
            proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of
            nuclear proteins related by the presence in each of a methyl-CpG
            binding domain (MBD). Each of these proteins, with the exception of
            MBD3, is capable of binding specifically to methylated DNA. MECP2,
            MBD1 and MBD2 can also repress transcription from methylated gene
            promoters. In contrast to other MBD family members, MECP2 is
            X-linked and subject to X inactivation. MECP2 is dispensible in
            stem cells, but is essential for embryonic development. MECP2 gene
            mutations are the cause of most cases of Rett syndrome, a
            progressive neurologic developmental disorder and one of the most
            common causes of cognitive disability in females. Alternative
            splicing results in multiple transcript variants encoding different
            isoforms. [provided by RefSeq, Oct 2015].
            
            Transcript Variant: This variant (6) lacks exon 2 and contains an
            additional exon, resulting in the use of a downstream start codon,
            compared to variant 1. The encoded isoform (3) has a shorter
            N-terminus compared to isoform 1. Variants 3-7 all encode the same
            isoform (3).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038197.141876.1,
                                           SRR14038193.1923753.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 5' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-114               AC244097.3         29110-29223         c
            115-226             AF030876.2         31264-31375         c
            227-577             AF030876.2         23679-24029         c
            578-3618            AF030876.2         19882-22922         c
            3619-10455          U52112.2           174507-181343       c
FEATURES             Location/Qualifiers
     source          1..10455
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq28"
     gene            1..10455
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="methyl-CpG binding protein 2"
                     /db_xref="GeneID:4204"
                     /db_xref="HGNC:HGNC:6990"
                     /db_xref="MIM:300005"
     exon            1..114
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     exon            115..226
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     exon            227..577
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     CDS             480..1661
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="isoform 3 is encoded by transcript variant 6;
                     meCp-2 protein"
                     /codon_start=1
                     /product="methyl-CpG-binding protein 2 isoform 3"
                     /protein_id="NP_001356322.1"
                     /db_xref="GeneID:4204"
                     /db_xref="HGNC:HGNC:6990"
                     /db_xref="MIM:300005"
                     /translation="MYDDPTLPEGWTRKLKQRKSGRSAGKYDVYLINPQGKAFRSKVE
                     LIAYFEKVGDTSLDPNDFDFTVTGRGSPSRREQKPPKKPKSPKAPGTGRGRGRPKGSG
                     TTRPKAATSEGVQVKRVLEKSPGKLLVKMPFQTSPGGKAEGGGATTSTQVMVIKRPGR
                     KRKAEADPQAIPKKRGRKPGSVVAAAAAEAKKKAVKESSIRSVQETVLPIKKRKTRET
                     VSIEVKEVVKPLLVSTLGEKSGKGLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHH
                     HHHSESPKAPVPLLPPLPPPPPEPESSEDPTSPPEPQDLSSSVCKEEKMPRGGSLESD
                     GCPKEPAKTQPAVATAATAAEKYKHRGEGERKDIVSSSMPRPNREEPVDSRTPVTERV
                     S"
     exon            578..10455
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10433..10438
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="hexamer: GATAAA"
     polyA_site      10455
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="major polyA site"
ORIGIN      
        1 gcgcgcgctc cctcctctcg gagagagggc tgtggtaaaa gccgtccgga aaatggccgc
       61 cgccgccgcc gccgcgccga gcggaggagg aggaggaggc gaggaggaga gactcactga
      121 gctagcctct ccttgctaga agcttacggt cgaaggtctc cagccatcag aagaagcacg
      181 tggagcgctg cgtccgtgtt gtggttattc atccagcatg tgttgaggaa gaaaagtcag
      241 aagaccagga cctccagggc ctcaaggaca aacccctcaa gtttaaaaag gtgaagaaag
      301 ataagaaaga agagaaagag ggcaagcatg agcccgtgca gccatcagcc caccactctg
      361 ctgagcccgc agaggcaggc aaagcagaga catcagaagg gtcaggctcc gccccggctg
      421 tgccggaagc ttctgcctcc cccaaacagc ggcgctccat catccgtgac cggggaccca
      481 tgtatgatga ccccaccctg cctgaaggct ggacacggaa gcttaagcaa aggaaatctg
      541 gccgctctgc tgggaagtat gatgtgtatt tgatcaatcc ccagggaaaa gcctttcgct
      601 ctaaagtgga gttgattgcg tacttcgaaa aggtaggcga cacatccctg gaccctaatg
      661 attttgactt cacggtaact gggagaggga gcccctcccg gcgagagcag aaaccaccta
      721 agaagcccaa atctcccaaa gctccaggaa ctggcagagg ccggggacgc cccaaaggga
      781 gcggcaccac gagacccaag gcggccacgt cagagggtgt gcaggtgaaa agggtcctgg
      841 agaaaagtcc tgggaagctc cttgtcaaga tgccttttca aacttcgcca gggggcaagg
      901 ctgagggggg tggggccacc acatccaccc aggtcatggt gatcaaacgc cccggcagga
      961 agcgaaaagc tgaggccgac cctcaggcca ttcccaagaa acggggccga aagccgggga
     1021 gtgtggtggc agccgctgcc gccgaggcca aaaagaaagc cgtgaaggag tcttctatcc
     1081 gatctgtgca ggagaccgta ctccccatca agaagcgcaa gacccgggag acggtcagca
     1141 tcgaggtcaa ggaagtggtg aagcccctgc tggtgtccac cctcggtgag aagagcggga
     1201 aaggactgaa gacctgtaag agccctgggc ggaaaagcaa ggagagcagc cccaaggggc
     1261 gcagcagcag cgcctcctca ccccccaaga aggagcacca ccaccatcac caccactcag
     1321 agtccccaaa ggcccccgtg ccactgctcc cacccctgcc cccacctcca cctgagcccg
     1381 agagctccga ggaccccacc agcccccctg agccccagga cttgagcagc agcgtctgca
     1441 aagaggagaa gatgcccaga ggaggctcac tggagagcga cggctgcccc aaggagccag
     1501 ctaagactca gcccgcggtt gccaccgccg ccacggccgc agaaaagtac aaacaccgag
     1561 gggagggaga gcgcaaagac attgtttcat cctccatgcc aaggccaaac agagaggagc
     1621 ctgtggacag ccggacgccc gtgaccgaga gagttagctg actttacacg gagcggattg
     1681 caaagcaaac caacaagaat aaaggcagct gttgtctctt ctccttatgg gtagggctct
     1741 gacaaagctt cccgattaac tgaaataaaa aatatttttt tttctttcag taaacttaga
     1801 gtttcgtggc ttcagggtgg gagtagttgg agcattgggg atgtttttct taccgacaag
     1861 cacagtcagg ttgaagacct aaccagggcc agaagtagct ttgcactttt ctaaactagg
     1921 ctccttcaac aaggcttgct gcagatacta ctgaccagac aagctgttga ccaggcacct
     1981 cccctcccgc ccaaaccttt cccccatgtg gtcgttagag acagagcgac agagcagttg
     2041 agaggacact cccgttttcg gtgccatcag tgccccgtct acagctcccc cagctccccc
     2101 cacctccccc actcccaacc acgttgggac agggaggtgt gaggcaggag agacagttgg
     2161 attctttaga gaagatggat atgaccagtg gctatggcct gtgcgatccc acccgtggtg
     2221 gctcaagtct ggccccacac cagccccaat ccaaaactgg caaggacgct tcacaggaca
     2281 ggaaagtggc acctgtctgc tccagctctg gcatggctag gaggggggag tcccttgaac
     2341 tactgggtgt agactggcct gaaccacagg agaggatggc ccagggtgag gtggcatggt
     2401 ccattctcaa gggacgtcct ccaacgggtg gcgctagagg ccatggaggc agtaggacaa
     2461 ggtgcaggca ggctggcctg gggtcaggcc gggcagagca cagcggggtg agagggattc
     2521 ctaatcactc agagcagtct gtgacttagt ggacagggga gggggcaaag ggggaggaga
     2581 agaaaatgtt cttccagtta ctttccaatt ctcctttagg gacagcttag aattatttgc
     2641 actattgagt cttcatgttc ccacttcaaa acaaacagat gctctgagag caaactggct
     2701 tgaattggtg acatttagtc cctcaagcca ccagatgtga cagtgttgag aactacctgg
     2761 atttgtatat atacctgcgc ttgttttaaa gtgggctcag cacatagggt tcccacgaag
     2821 ctccgaaact ctaagtgttt gctgcaattt tataaggact tcctgattgg tttctcttct
     2881 ccccttccat ttctgccttt tgttcatttc atcctttcac ttctttccct tcctccatcc
     2941 tcctccttcc tagttcatcc cttctcttcc aggcagccgc ggtgcccaac cacacttgtc
     3001 ggctccagtc cccagaactc tgcctgccct ttgtcctcct gctgccagta ccagccccac
     3061 cctgttttga gccctgagga ggccttgggc tctgctgagt ccgacctggc ctgtctgtga
     3121 agagcaagag agcagcaagg tcttgctctc ctaggtagcc ccctcttccc tggtaagaaa
     3181 aagcaaaagg catttcccac cctgaacaac gagccttttc acccttctac tctagagaag
     3241 tggactggag gagctgggcc cgatttggta gttgaggaaa gcacagaggc ctcctgtggc
     3301 ctgccagtca tcgagtggcc caacaggggc tccatgccag ccgaccttga cctcactcag
     3361 aagtccagag tctagcgtag tgcagcaggg cagtagcggt accaatgcag aactcccaag
     3421 acccgagctg ggaccagtac ctgggtcccc agcccttcct ctgctccccc ttttccctcg
     3481 gagttcttct tgaatggcaa tgttttgctt ttgctcgatg cagacagggg gccagaacac
     3541 cacacatttc actgtctgtc tggtccatag ctgtggtgta ggggcttaga ggcatgggct
     3601 tgctgtgggt ttttaattga tcagttttca tgtgggatcc catcttttta acctctgttc
     3661 aggaagtcct tatctagctg catatcttca tcatattggt atatcctttt ctgtgtttac
     3721 agagatgtct cttatatcta aatctgtcca actgagaagt accttatcaa agtagcaaat
     3781 gagacagcag tcttatgctt ccagaaacac ccacaggcat gtcccatgtg agctgctgcc
     3841 atgaactgtc aagtgtgtgt tgtcttgtgt atttcagtta ttgtccctgg cttccttact
     3901 atggtgtaat catgaaggag tgaaacatca tagaaactgt ctagcacttc cttgccagtc
     3961 tttagtgatc aggaaccata gttgacagtt ccaatcagta gcttaagaaa aaaccgtgtt
     4021 tgtctcttct ggaatggtta gaagtgaggg agtttgcccc gttctgtttg tagagtctca
     4081 tagttggact ttctagcata tatgtgtcca tttccttatg ctgtaaaagc aagtcctgca
     4141 accaaactcc catcagccca atccctgatc cctgatccct tccacctgct ctgctgatga
     4201 cccccccagc ttcacttctg actcttcccc aggaagggaa ggggggtcag aagagagggt
     4261 gagtcctcca gaactcttcc tccaaggaca gaaggctcct gcccccatag tggcctcgaa
     4321 ctcctggcac taccaaagga cacttatcca cgagagcgca gcatccgacc aggttgtcac
     4381 tgagaagatg tttattttgg tcagttgggt ttttatgtat tatacttagt caaatgtaat
     4441 gtggcttctg gaatcattgt ccagagctgc ttccccgtca cctgggcgtc atctggtcct
     4501 ggtaagagga gtgcgtggcc caccaggccc ccctgtcacc catgacagtt cattcagggc
     4561 cgatggggca gtcgtggttg ggaacacagc atttcaagcg tcactttatt tcattcgggc
     4621 cccacctgca gctccctcaa agaggcagtt gcccagcctc tttcccttcc agtttattcc
     4681 agagctgcca gtggggcctg aggctcctta gggttttctc tctatttccc cctttcttcc
     4741 tcattccctc gtctttccca aaggcatcac gagtcagtcg cctttcagca ggcagccttg
     4801 gcggtttatc gccctggcag gcaggggccc tgcagctctc atgctgcccc tgccttgggg
     4861 tcaggttgac aggaggttgg agggaaagcc ttaagctgca ggattctcac cagctgtgtc
     4921 cggcccagtt ttggggtgtg acctcaattt caattttgtc tgtacttgaa cattatgaag
     4981 atgggggcct ctttcagtga atttgtgaac agcagaattg accgacagct ttccagtacc
     5041 catggggcta ggtcattaag gccacatcca cagtctcccc cacccttgtt ccagttgtta
     5101 gttactacct cctctcctga caatactgta tgtcgtcgag ctccccccag gtctacccct
     5161 cccggccctg cctgctggtg ggcttgtcat agccagtggg attgccggtc ttgacagctc
     5221 agtgagctgg agatacttgg tcacagccag gcgctagcac agctcccttc tgttgatgct
     5281 gtattcccat atcaaaagac acaggggaca cccagaaacg ccacatcccc caatccatca
     5341 gtgccaaact agccaacggc cccagcttct cagctcgctg gatggcggaa gctgctactc
     5401 gtgagcgcca gtgcgggtgc agacaatctt ctgttgggtg gcatcattcc aggcccgaag
     5461 catgaacagt gcacctggga cagggagcag ccccaaattg tcacctgctt ctctgcccag
     5521 cttttcattg ctgtgacagt gatggcgaaa gagggtaata accagacaca aactgccaag
     5581 ttgggtggag aaaggagttt ctttagctga cagaatctct gaattttaaa tcacttagta
     5641 agcggctcaa gcccaggagg gagcagaggg atacgagcgg agtcccctgc gcgggaccat
     5701 ctggaattgg tttagcccaa gtggagcctg acagccagaa ctctgtgtcc cccgtctaac
     5761 cacagctcct tttccagagc attccagtca ggctctctgg gctgactggg ccaggggagg
     5821 ttacaggtac cagttcttta agaagatctt tgggcatata catttttagc ctgtgtcatt
     5881 gccccaaatg gattcctgtt tcaagttcac acctgcagat tctaggacct gtgtcctaga
     5941 cttcagggag tcagctgttt ctagagttcc taccatggag tgggtctgga ggacctgccc
     6001 ggtggggggg cagagccctg ctccctccgg gtcttcctac tcttctctct gctctgacgg
     6061 gatttgttga ttctctccat tttggtgtct ttctctttta gatattgtat caatctttag
     6121 aaaaggcata gtctacttgt tataaatcgt taggatactg cctcccccag ggtctaaaat
     6181 tacatattag aggggaaaag ctgaacactg aagtcagttc tcaacaattt agaaggaaaa
     6241 cctagaaaac atttggcaga aaattacatt tcgatgtttt tgaatgaata cgagcaagct
     6301 tttacaacag tgctgatcta aaaatactta gcacttggcc tgagatgcct ggtgagcatt
     6361 acaggcaagg ggaatctgga ggtagccgac ctgaggacat ggcttctgaa cctgtctttt
     6421 gggagtggta tggaaggtgg agcgttcacc agtgacctgg aaggcccagc accaccctcc
     6481 ttcccactct tctcatcttg acagagcctg ccccagcgct gacgtgtcag gaaaacaccc
     6541 agggaactag gaaggcactt ctgcctgagg ggcagcctgc cttgcccact cctgctctgc
     6601 tcgcctcgga tcagctgagc cttctgagct ggcctctcac tgcctcccca aggccccctg
     6661 cctgccctgt caggaggcag aaggaagcag gtgtgagggc agtgcaagga gggagcacaa
     6721 cccccagctc ccgctccggg ctccgacttg tgcacaggca gagcccagac cctggaggaa
     6781 atcctacctt tgaattcaag aacatttggg gaatttggaa atctctttgc ccccaaaccc
     6841 ccattctgtc ctacctttaa tcaggtcctg ctcagcagtg agagcagatg aggtgaaaag
     6901 gccaagaggt ttggctcctg cccactgata gcccctctcc ccgcagtgtt tgtgtgtcaa
     6961 gtggcaaagc tgttcttcct ggtgaccctg attatatcca gtaacacata gactgtgcgc
     7021 ataggcctgc tttgtctcct ctatcctggg cttttgtttt gctttttagt tttgctttta
     7081 gtttttctgt cccttttatt taacgcaccg actagacaca caaagcagtt gaatttttat
     7141 atatatatct gtatattgca caattataaa ctcattttgc ttgtggctcc acacacacaa
     7201 aaaaagacct gttaaaatta tacctgttgc ttaattacaa tatttctgat aaccatagca
     7261 taggacaagg gaaaataaaa aaagaaaaaa aagaaaaaaa aacgacaaat ctgtctgctg
     7321 gtcacttctt ctgtccaagc agattcgtgg tcttttcctc gcttctttca agggctttcc
     7381 tgtgccaggt gaaggaggct ccaggcagca cccaggtttt gcactcttgt ttctcccgtg
     7441 cttgtgaaag aggtcccaag gttctgggtg caggagcgct cccttgacct gctgaagtcc
     7501 ggaacgtagt cggcacagcc tggtcgcctt ccacctctgg gagctggagt ccactggggt
     7561 ggcctgactc ccccagtccc cttcccgtga cctggtcagg gtgagcccat gtggagtcag
     7621 cctcgcaggc ctccctgcca gtagggtccg agtgtgtttc atccttccca ctctgtcgag
     7681 cctgggggct ggagcggaga cgggaggcct ggcctgtctc ggaacctgtg agctgcacca
     7741 ggtagaacgc cagggacccc agaatcatgt gcgtcagtcc aaggggtccc ctccaggagt
     7801 agtgaagact ccagaaatgt ccctttcttc tcccccatcc tacgagtaat tgcatttgct
     7861 tttgtaattc ttaatgagca atatctgcta gagagtttag ctgtaacagt tctttttgat
     7921 catctttttt taataattag aaacaccaaa aaaatccaga aacttgttct tccaaagcag
     7981 agagcattat aatcaccagg gccaaaagct tccctccctg ctgtcattgc ttcttctgag
     8041 gcctgaatcc aaaagaaaaa cagccatagg ccctttcagt ggccgggcta cccgtgagcc
     8101 cttcggagga ccagggctgg ggcagcctct gggcccacat ccggggccag ctccggcgtg
     8161 tgttcagtgt tagcagtggg tcatgatgct ctttcccacc cagcctggga taggggcaga
     8221 ggaggcgagg aggccgttgc cgctgatgtt tggccgtgaa caggtgggtg tctgcgtgcg
     8281 tccacgtgcg tgttttctga ctgacatgaa atcgacgccc gagttagcct cacccggtga
     8341 cctctagccc tgcccggatg gagcggggcc cacccggttc agtgtttctg gggagctgga
     8401 cagtggagtg caaaaggctt gcagaacttg aagcctgctc cttcccttgc taccacggcc
     8461 tcctttccgt ttgatttgtc actgcttcaa tcaataacag ccgctccaga gtcagtagtc
     8521 aatgaatata tgaccaaata tcaccaggac tgttactcaa tgtgtgccga gcccttgccc
     8581 atgctgggct cccgtgtatc tggacactgt aacgtgtgct gtgtttgctc cccttcccct
     8641 tccttctttg ccctttactt gtctttctgg ggtttttctg tttgggtttg gtttggtttt
     8701 tatttctcct tttgtgttcc aaacatgagg ttctctctac tggtcctctt aactgtggtg
     8761 ttgaggctta tatttgtgta atttttggtg ggtgaaagga attttgctaa gtaaatctct
     8821 tctgtgtttg aactgaagtc tgtattgtaa ctatgtttaa agtaattgtt ccagagacaa
     8881 atatttctag acactttttc tttacaaaca aaagcattcg gagggagggg gatggtgact
     8941 gagatgagag gggagagctg aacagatgac ccctgcccag atcagccaga agccacccaa
     9001 agcagtggag cccaggagtc ccactccaag ccagcaagcc gaatagctga tgtgttgcca
     9061 ctttccaagt cactgcaaaa ccaggttttg ttccgcccag tggattcttg ttttgcttcc
     9121 cctccccccg agattattac caccatcccg tgcttttaag gaaaggcaag attgatgttt
     9181 ccttgagggg agccaggagg ggatgtgtgt gtgcagagct gaagagctgg ggagaatggg
     9241 gctgggccca cccaagcagg aggctgggac gctctgctgt gggcacaggt caggctaatg
     9301 ttggcagatg cagctcttcc tggacaggcc aggtggtggg cattctctct ccaaggtgtg
     9361 ccccgtgggc attactgttt aagacacttc cgtcacatcc caccccatcc tccagggctc
     9421 aacactgtga catctctatt ccccaccctc cccttcccag ggcaataaaa tgaccatgga
     9481 gggggcttgc actctcttgg ctgtcacccg atcgccagca aaacttagat gtgagaaaac
     9541 cccttcccat tccatggcga aaacatctcc ttagaaaagc cattaccctc attaggcatg
     9601 gttttgggct cccaaaacac ctgacagccc ctccctcctc tgagaggcgg agagtgctga
     9661 ctgtagtgac cattgcatgc cgggtgcagc atctggaaga gctaggcagg gtgtctgccc
     9721 cctcctgagt tgaagtcatg ctcccctgtg ccagcccaga ggccgagagc tatggacagc
     9781 attgccagta acacaggcca ccctgtgcag aagggagctg gctccagcct ggaaacctgt
     9841 ctgaggttgg gagaggtgca cttggggcac agggagaggc cgggacacac ttagctggag
     9901 atgtctctaa aagccctgta tcgtattcac cttcagtttt tgtgttttgg gacaattact
     9961 ttagaaaata agtaggtcgt tttaaaaaca aaaattattg attgcttttt tgtagtgttc
    10021 agaaaaaagg ttctttgtgt atagccaaat gactgaaagc actgatatat ttaaaaacaa
    10081 aaggcaattt attaaggaaa tttgtaccat ttcagtaaac ctgtctgaat gtacctgtat
    10141 acgtttcaaa aacacccccc ccccactgaa tccctgtaac ctatttatta tataaagagt
    10201 ttgccttata aatttacata aaaatgtccg tttgtgtctt ttgttgtaaa aatcaagtga
    10261 ttttttcata aggttctttt actattggaa aagatgggca gcacgcagtt ttattttatt
    10321 tttgtaagtt ttttaataca tgtgaaagca aagaatactc agcatgcctt tctaagtgac
    10381 gcgtttgcac cttttgttgg gaagtactgt atcctgtgct gttagcattc tcgataaatc
    10441 tctctgtgaa agtga
//
