LOCUS       NM_001369392           10579 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens methyl-CpG binding protein 2 (MECP2), transcript
            variant 5, mRNA.
ACCESSION   NM_001369392 XM_024452384
VERSION     NM_001369392.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10579)
  AUTHORS   Tomasello,D.L., Barrasa,M.I., Mankus,D., Alarcon,K.I.,
            Lytton-Jean,A.K.R., Liu,X.S. and Jaenisch,R.
  TITLE     Mitochondrial dysfunction and increased reactive oxygen species
            production in MECP2 mutant astrocytes and their impact on neurons
  JOURNAL   Sci Rep 14 (1), 20565 (2024)
   PUBMED   39232000
  REMARK    GeneRIF: Mitochondrial dysfunction and increased reactive oxygen
            species production in MECP2 mutant astrocytes and their impact on
            neurons.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10579)
  AUTHORS   Fichou,Y., Nectoux,J., Bahi-Buisson,N., Rosas-Vargas,H., Girard,B.,
            Chelly,J. and Bienvenu,T.
  TITLE     The first missense mutation causing Rett syndrome specifically
            affecting the MeCP2_e1 isoform
  JOURNAL   Neurogenetics 10 (2), 127-133 (2009)
   PUBMED   19034540
  REMARK    GeneRIF: Data suggest the important involvement of the N-terminus
            in the function of MeCP2 protein, and provide further evidence for
            the major impact of a specific MeCP2e_1 deficiency in the
            development of intellectual processing.
REFERENCE   3  (bases 1 to 10579)
  AUTHORS   Harvey,C.G., Menon,S.D., Stachowiak,B., Noor,A., Proctor,A.,
            Mensah,A.K., Mnatzakanian,G.N., Alfred,S.E., Guo,R., Scherer,S.W.,
            Kennedy,J.L., Roberts,W., Srivastava,A.K., Minassian,B.A. and
            Vincent,J.B.
  TITLE     Sequence variants within exon 1 of MECP2 occur in females with
            mental retardation
  JOURNAL   Am J Med Genet B Neuropsychiatr Genet 144B (3), 355-360 (2007)
   PUBMED   17171659
  REMARK    GeneRIF: polymorphisms within exon 1 of MECP2 occur in females with
            mental retardation.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Erratum:[Am J Med Genet B Neuropsychiatr Genet. 2007 Oct
            5;144(7):969. Srivistava, Anand K [corrected to Srivastava, Anand
            K]]
REFERENCE   4  (bases 1 to 10579)
  AUTHORS   Francke,U.
  TITLE     Mechanisms of disease: neurogenetics of MeCP2 deficiency
  JOURNAL   Nat Clin Pract Neurol 2 (4), 212-221 (2006)
   PUBMED   16932552
  REMARK    GeneRIF: Rett syndrome is caused by heterozygosity for mutations in
            the X-linked gene MECP2, which encodes methyl-CpG binding protein
            2.
            Review article
REFERENCE   5  (bases 1 to 10579)
  AUTHORS   Mnatzakanian,G.N., Lohi,H., Munteanu,I., Alfred,S.E., Yamada,T.,
            MacLeod,P.J., Jones,J.R., Scherer,S.W., Schanen,N.C., Friez,M.J.,
            Vincent,J.B. and Minassian,B.A.
  TITLE     A previously unidentified MECP2 open reading frame defines a new
            protein isoform relevant to Rett syndrome
  JOURNAL   Nat Genet 36 (4), 339-341 (2004)
   PUBMED   15034579
  REMARK    GeneRIF: description of a previously unknown MeCP2 isoform
            Erratum:[Nat Genet. 2004 May;36(5):540]
REFERENCE   6  (bases 1 to 10579)
  AUTHORS   Reichwald,K., Thiesen,J., Wiehe,T., Weitzel,J., Poustka,W.A.,
            Rosenthal,A., Platzer,M., Stratling,W.H. and Kioschis,P.
  TITLE     Comparative sequence analysis of the MECP2-locus in human and mouse
            reveals new transcribed regions
  JOURNAL   Mamm Genome 11 (3), 182-190 (2000)
   PUBMED   10723722
REFERENCE   7  (bases 1 to 10579)
  AUTHORS   Kaur,S. and Christodoulou,J.
  TITLE     MECP2 Disorders
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301670
REFERENCE   8  (bases 1 to 10579)
  AUTHORS   Van Esch,H.
  TITLE     MECP2 Duplication Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301461
REFERENCE   9  (bases 1 to 10579)
  AUTHORS   Klein,C., Lohmann,K., Marras,C. and Munchau,A.
  TITLE     Hereditary Dystonia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301334
REFERENCE   10 (bases 1 to 10579)
  AUTHORS   Lewis,J.D., Meehan,R.R., Henzel,W.J., Maurer-Fogy,I., Jeppesen,P.,
            Klein,F. and Bird,A.
  TITLE     Purification, sequence, and cellular localization of a novel
            chromosomal protein that binds to methylated DNA
  JOURNAL   Cell 69 (6), 905-914 (1992)
   PUBMED   1606614
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC244097.3, AF030876.2 and
            U52112.2.
            
            On Jun 2, 2019 this sequence version replaced NM_001369392.1.
            
            Summary: DNA methylation is the major modification of eukaryotic
            genomes and plays an essential role in mammalian development. Human
            proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of
            nuclear proteins related by the presence in each of a methyl-CpG
            binding domain (MBD). Each of these proteins, with the exception of
            MBD3, is capable of binding specifically to methylated DNA. MECP2,
            MBD1 and MBD2 can also repress transcription from methylated gene
            promoters. In contrast to other MBD family members, MECP2 is
            X-linked and subject to X inactivation. MECP2 is dispensible in
            stem cells, but is essential for embryonic development. MECP2 gene
            mutations are the cause of most cases of Rett syndrome, a
            progressive neurologic developmental disorder and one of the most
            common causes of cognitive disability in females. Alternative
            splicing results in multiple transcript variants encoding different
            isoforms. [provided by RefSeq, Oct 2015].
            
            Transcript Variant: This variant (5) includes exon 2 and an
            additional exon, resulting in the use of a downstream start codon,
            compared to variant 1. The encoded isoform (3) has a shorter
            N-terminus compared to isoform 1. Variants 3-7 all encode the same
            isoform (3).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: SRR7346977.2268639.1, X89430.1
                                    [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMEA2467144 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 5' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-114               AC244097.3         29110-29223         c
            115-238             AC244097.3         23690-23813         c
            239-350             AF030876.2         31264-31375         c
            351-701             AF030876.2         23679-24029         c
            702-3742            AF030876.2         19882-22922         c
            3743-10579          U52112.2           174507-181343       c
FEATURES             Location/Qualifiers
     source          1..10579
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq28"
     gene            1..10579
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="methyl-CpG binding protein 2"
                     /db_xref="GeneID:4204"
                     /db_xref="HGNC:HGNC:6990"
                     /db_xref="MIM:300005"
     exon            1..114
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     exon            115..238
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     exon            239..350
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     exon            351..701
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     CDS             604..1785
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="isoform 3 is encoded by transcript variant 5;
                     meCp-2 protein"
                     /codon_start=1
                     /product="methyl-CpG-binding protein 2 isoform 3"
                     /protein_id="NP_001356321.1"
                     /db_xref="GeneID:4204"
                     /db_xref="HGNC:HGNC:6990"
                     /db_xref="MIM:300005"
                     /translation="MYDDPTLPEGWTRKLKQRKSGRSAGKYDVYLINPQGKAFRSKVE
                     LIAYFEKVGDTSLDPNDFDFTVTGRGSPSRREQKPPKKPKSPKAPGTGRGRGRPKGSG
                     TTRPKAATSEGVQVKRVLEKSPGKLLVKMPFQTSPGGKAEGGGATTSTQVMVIKRPGR
                     KRKAEADPQAIPKKRGRKPGSVVAAAAAEAKKKAVKESSIRSVQETVLPIKKRKTRET
                     VSIEVKEVVKPLLVSTLGEKSGKGLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHH
                     HHHSESPKAPVPLLPPLPPPPPEPESSEDPTSPPEPQDLSSSVCKEEKMPRGGSLESD
                     GCPKEPAKTQPAVATAATAAEKYKHRGEGERKDIVSSSMPRPNREEPVDSRTPVTERV
                     S"
     exon            702..10579
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10557..10562
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="hexamer: GATAAA"
     polyA_site      10579
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="major polyA site"
ORIGIN      
        1 gcgcgcgctc cctcctctcg gagagagggc tgtggtaaaa gccgtccgga aaatggccgc
       61 cgccgccgcc gccgcgccga gcggaggagg aggaggaggc gaggaggaga gactgctcca
      121 taaaaataca gactcaccag ttcctgcttt gatgtgacat gtgactcccc agaatacacc
      181 ttgcttctgt agaccagctc caacaggatt ccatggtagc tgggatgtta gggctcagca
      241 ctgagctagc ctctccttgc tagaagctta cggtcgaagg tctccagcca tcagaagaag
      301 cacgtggagc gctgcgtccg tgttgtggtt attcatccag catgtgttga ggaagaaaag
      361 tcagaagacc aggacctcca gggcctcaag gacaaacccc tcaagtttaa aaaggtgaag
      421 aaagataaga aagaagagaa agagggcaag catgagcccg tgcagccatc agcccaccac
      481 tctgctgagc ccgcagaggc aggcaaagca gagacatcag aagggtcagg ctccgccccg
      541 gctgtgccgg aagcttctgc ctcccccaaa cagcggcgct ccatcatccg tgaccgggga
      601 cccatgtatg atgaccccac cctgcctgaa ggctggacac ggaagcttaa gcaaaggaaa
      661 tctggccgct ctgctgggaa gtatgatgtg tatttgatca atccccaggg aaaagccttt
      721 cgctctaaag tggagttgat tgcgtacttc gaaaaggtag gcgacacatc cctggaccct
      781 aatgattttg acttcacggt aactgggaga gggagcccct cccggcgaga gcagaaacca
      841 cctaagaagc ccaaatctcc caaagctcca ggaactggca gaggccgggg acgccccaaa
      901 gggagcggca ccacgagacc caaggcggcc acgtcagagg gtgtgcaggt gaaaagggtc
      961 ctggagaaaa gtcctgggaa gctccttgtc aagatgcctt ttcaaacttc gccagggggc
     1021 aaggctgagg ggggtggggc caccacatcc acccaggtca tggtgatcaa acgccccggc
     1081 aggaagcgaa aagctgaggc cgaccctcag gccattccca agaaacgggg ccgaaagccg
     1141 gggagtgtgg tggcagccgc tgccgccgag gccaaaaaga aagccgtgaa ggagtcttct
     1201 atccgatctg tgcaggagac cgtactcccc atcaagaagc gcaagacccg ggagacggtc
     1261 agcatcgagg tcaaggaagt ggtgaagccc ctgctggtgt ccaccctcgg tgagaagagc
     1321 gggaaaggac tgaagacctg taagagccct gggcggaaaa gcaaggagag cagccccaag
     1381 gggcgcagca gcagcgcctc ctcacccccc aagaaggagc accaccacca tcaccaccac
     1441 tcagagtccc caaaggcccc cgtgccactg ctcccacccc tgcccccacc tccacctgag
     1501 cccgagagct ccgaggaccc caccagcccc cctgagcccc aggacttgag cagcagcgtc
     1561 tgcaaagagg agaagatgcc cagaggaggc tcactggaga gcgacggctg ccccaaggag
     1621 ccagctaaga ctcagcccgc ggttgccacc gccgccacgg ccgcagaaaa gtacaaacac
     1681 cgaggggagg gagagcgcaa agacattgtt tcatcctcca tgccaaggcc aaacagagag
     1741 gagcctgtgg acagccggac gcccgtgacc gagagagtta gctgacttta cacggagcgg
     1801 attgcaaagc aaaccaacaa gaataaaggc agctgttgtc tcttctcctt atgggtaggg
     1861 ctctgacaaa gcttcccgat taactgaaat aaaaaatatt tttttttctt tcagtaaact
     1921 tagagtttcg tggcttcagg gtgggagtag ttggagcatt ggggatgttt ttcttaccga
     1981 caagcacagt caggttgaag acctaaccag ggccagaagt agctttgcac ttttctaaac
     2041 taggctcctt caacaaggct tgctgcagat actactgacc agacaagctg ttgaccaggc
     2101 acctcccctc ccgcccaaac ctttccccca tgtggtcgtt agagacagag cgacagagca
     2161 gttgagagga cactcccgtt ttcggtgcca tcagtgcccc gtctacagct cccccagctc
     2221 cccccacctc ccccactccc aaccacgttg ggacagggag gtgtgaggca ggagagacag
     2281 ttggattctt tagagaagat ggatatgacc agtggctatg gcctgtgcga tcccacccgt
     2341 ggtggctcaa gtctggcccc acaccagccc caatccaaaa ctggcaagga cgcttcacag
     2401 gacaggaaag tggcacctgt ctgctccagc tctggcatgg ctaggagggg ggagtccctt
     2461 gaactactgg gtgtagactg gcctgaacca caggagagga tggcccaggg tgaggtggca
     2521 tggtccattc tcaagggacg tcctccaacg ggtggcgcta gaggccatgg aggcagtagg
     2581 acaaggtgca ggcaggctgg cctggggtca ggccgggcag agcacagcgg ggtgagaggg
     2641 attcctaatc actcagagca gtctgtgact tagtggacag gggagggggc aaagggggag
     2701 gagaagaaaa tgttcttcca gttactttcc aattctcctt tagggacagc ttagaattat
     2761 ttgcactatt gagtcttcat gttcccactt caaaacaaac agatgctctg agagcaaact
     2821 ggcttgaatt ggtgacattt agtccctcaa gccaccagat gtgacagtgt tgagaactac
     2881 ctggatttgt atatatacct gcgcttgttt taaagtgggc tcagcacata gggttcccac
     2941 gaagctccga aactctaagt gtttgctgca attttataag gacttcctga ttggtttctc
     3001 ttctcccctt ccatttctgc cttttgttca tttcatcctt tcacttcttt cccttcctcc
     3061 atcctcctcc ttcctagttc atcccttctc ttccaggcag ccgcggtgcc caaccacact
     3121 tgtcggctcc agtccccaga actctgcctg ccctttgtcc tcctgctgcc agtaccagcc
     3181 ccaccctgtt ttgagccctg aggaggcctt gggctctgct gagtccgacc tggcctgtct
     3241 gtgaagagca agagagcagc aaggtcttgc tctcctaggt agccccctct tccctggtaa
     3301 gaaaaagcaa aaggcatttc ccaccctgaa caacgagcct tttcaccctt ctactctaga
     3361 gaagtggact ggaggagctg ggcccgattt ggtagttgag gaaagcacag aggcctcctg
     3421 tggcctgcca gtcatcgagt ggcccaacag gggctccatg ccagccgacc ttgacctcac
     3481 tcagaagtcc agagtctagc gtagtgcagc agggcagtag cggtaccaat gcagaactcc
     3541 caagacccga gctgggacca gtacctgggt ccccagccct tcctctgctc ccccttttcc
     3601 ctcggagttc ttcttgaatg gcaatgtttt gcttttgctc gatgcagaca gggggccaga
     3661 acaccacaca tttcactgtc tgtctggtcc atagctgtgg tgtaggggct tagaggcatg
     3721 ggcttgctgt gggtttttaa ttgatcagtt ttcatgtggg atcccatctt tttaacctct
     3781 gttcaggaag tccttatcta gctgcatatc ttcatcatat tggtatatcc ttttctgtgt
     3841 ttacagagat gtctcttata tctaaatctg tccaactgag aagtacctta tcaaagtagc
     3901 aaatgagaca gcagtcttat gcttccagaa acacccacag gcatgtccca tgtgagctgc
     3961 tgccatgaac tgtcaagtgt gtgttgtctt gtgtatttca gttattgtcc ctggcttcct
     4021 tactatggtg taatcatgaa ggagtgaaac atcatagaaa ctgtctagca cttccttgcc
     4081 agtctttagt gatcaggaac catagttgac agttccaatc agtagcttaa gaaaaaaccg
     4141 tgtttgtctc ttctggaatg gttagaagtg agggagtttg ccccgttctg tttgtagagt
     4201 ctcatagttg gactttctag catatatgtg tccatttcct tatgctgtaa aagcaagtcc
     4261 tgcaaccaaa ctcccatcag cccaatccct gatccctgat cccttccacc tgctctgctg
     4321 atgacccccc cagcttcact tctgactctt ccccaggaag ggaagggggg tcagaagaga
     4381 gggtgagtcc tccagaactc ttcctccaag gacagaaggc tcctgccccc atagtggcct
     4441 cgaactcctg gcactaccaa aggacactta tccacgagag cgcagcatcc gaccaggttg
     4501 tcactgagaa gatgtttatt ttggtcagtt gggtttttat gtattatact tagtcaaatg
     4561 taatgtggct tctggaatca ttgtccagag ctgcttcccc gtcacctggg cgtcatctgg
     4621 tcctggtaag aggagtgcgt ggcccaccag gcccccctgt cacccatgac agttcattca
     4681 gggccgatgg ggcagtcgtg gttgggaaca cagcatttca agcgtcactt tatttcattc
     4741 gggccccacc tgcagctccc tcaaagaggc agttgcccag cctctttccc ttccagttta
     4801 ttccagagct gccagtgggg cctgaggctc cttagggttt tctctctatt tccccctttc
     4861 ttcctcattc cctcgtcttt cccaaaggca tcacgagtca gtcgcctttc agcaggcagc
     4921 cttggcggtt tatcgccctg gcaggcaggg gccctgcagc tctcatgctg cccctgcctt
     4981 ggggtcaggt tgacaggagg ttggagggaa agccttaagc tgcaggattc tcaccagctg
     5041 tgtccggccc agttttgggg tgtgacctca atttcaattt tgtctgtact tgaacattat
     5101 gaagatgggg gcctctttca gtgaatttgt gaacagcaga attgaccgac agctttccag
     5161 tacccatggg gctaggtcat taaggccaca tccacagtct cccccaccct tgttccagtt
     5221 gttagttact acctcctctc ctgacaatac tgtatgtcgt cgagctcccc ccaggtctac
     5281 ccctcccggc cctgcctgct ggtgggcttg tcatagccag tgggattgcc ggtcttgaca
     5341 gctcagtgag ctggagatac ttggtcacag ccaggcgcta gcacagctcc cttctgttga
     5401 tgctgtattc ccatatcaaa agacacaggg gacacccaga aacgccacat cccccaatcc
     5461 atcagtgcca aactagccaa cggccccagc ttctcagctc gctggatggc ggaagctgct
     5521 actcgtgagc gccagtgcgg gtgcagacaa tcttctgttg ggtggcatca ttccaggccc
     5581 gaagcatgaa cagtgcacct gggacaggga gcagccccaa attgtcacct gcttctctgc
     5641 ccagcttttc attgctgtga cagtgatggc gaaagagggt aataaccaga cacaaactgc
     5701 caagttgggt ggagaaagga gtttctttag ctgacagaat ctctgaattt taaatcactt
     5761 agtaagcggc tcaagcccag gagggagcag agggatacga gcggagtccc ctgcgcggga
     5821 ccatctggaa ttggtttagc ccaagtggag cctgacagcc agaactctgt gtcccccgtc
     5881 taaccacagc tccttttcca gagcattcca gtcaggctct ctgggctgac tgggccaggg
     5941 gaggttacag gtaccagttc tttaagaaga tctttgggca tatacatttt tagcctgtgt
     6001 cattgcccca aatggattcc tgtttcaagt tcacacctgc agattctagg acctgtgtcc
     6061 tagacttcag ggagtcagct gtttctagag ttcctaccat ggagtgggtc tggaggacct
     6121 gcccggtggg ggggcagagc cctgctccct ccgggtcttc ctactcttct ctctgctctg
     6181 acgggatttg ttgattctct ccattttggt gtctttctct tttagatatt gtatcaatct
     6241 ttagaaaagg catagtctac ttgttataaa tcgttaggat actgcctccc ccagggtcta
     6301 aaattacata ttagagggga aaagctgaac actgaagtca gttctcaaca atttagaagg
     6361 aaaacctaga aaacatttgg cagaaaatta catttcgatg tttttgaatg aatacgagca
     6421 agcttttaca acagtgctga tctaaaaata cttagcactt ggcctgagat gcctggtgag
     6481 cattacaggc aaggggaatc tggaggtagc cgacctgagg acatggcttc tgaacctgtc
     6541 ttttgggagt ggtatggaag gtggagcgtt caccagtgac ctggaaggcc cagcaccacc
     6601 ctccttccca ctcttctcat cttgacagag cctgccccag cgctgacgtg tcaggaaaac
     6661 acccagggaa ctaggaaggc acttctgcct gaggggcagc ctgccttgcc cactcctgct
     6721 ctgctcgcct cggatcagct gagccttctg agctggcctc tcactgcctc cccaaggccc
     6781 cctgcctgcc ctgtcaggag gcagaaggaa gcaggtgtga gggcagtgca aggagggagc
     6841 acaaccccca gctcccgctc cgggctccga cttgtgcaca ggcagagccc agaccctgga
     6901 ggaaatccta cctttgaatt caagaacatt tggggaattt ggaaatctct ttgcccccaa
     6961 acccccattc tgtcctacct ttaatcaggt cctgctcagc agtgagagca gatgaggtga
     7021 aaaggccaag aggtttggct cctgcccact gatagcccct ctccccgcag tgtttgtgtg
     7081 tcaagtggca aagctgttct tcctggtgac cctgattata tccagtaaca catagactgt
     7141 gcgcataggc ctgctttgtc tcctctatcc tgggcttttg ttttgctttt tagttttgct
     7201 tttagttttt ctgtcccttt tatttaacgc accgactaga cacacaaagc agttgaattt
     7261 ttatatatat atctgtatat tgcacaatta taaactcatt ttgcttgtgg ctccacacac
     7321 acaaaaaaag acctgttaaa attatacctg ttgcttaatt acaatatttc tgataaccat
     7381 agcataggac aagggaaaat aaaaaaagaa aaaaaagaaa aaaaaacgac aaatctgtct
     7441 gctggtcact tcttctgtcc aagcagattc gtggtctttt cctcgcttct ttcaagggct
     7501 ttcctgtgcc aggtgaagga ggctccaggc agcacccagg ttttgcactc ttgtttctcc
     7561 cgtgcttgtg aaagaggtcc caaggttctg ggtgcaggag cgctcccttg acctgctgaa
     7621 gtccggaacg tagtcggcac agcctggtcg ccttccacct ctgggagctg gagtccactg
     7681 gggtggcctg actcccccag tccccttccc gtgacctggt cagggtgagc ccatgtggag
     7741 tcagcctcgc aggcctccct gccagtaggg tccgagtgtg tttcatcctt cccactctgt
     7801 cgagcctggg ggctggagcg gagacgggag gcctggcctg tctcggaacc tgtgagctgc
     7861 accaggtaga acgccaggga ccccagaatc atgtgcgtca gtccaagggg tcccctccag
     7921 gagtagtgaa gactccagaa atgtcccttt cttctccccc atcctacgag taattgcatt
     7981 tgcttttgta attcttaatg agcaatatct gctagagagt ttagctgtaa cagttctttt
     8041 tgatcatctt tttttaataa ttagaaacac caaaaaaatc cagaaacttg ttcttccaaa
     8101 gcagagagca ttataatcac cagggccaaa agcttccctc cctgctgtca ttgcttcttc
     8161 tgaggcctga atccaaaaga aaaacagcca taggcccttt cagtggccgg gctacccgtg
     8221 agcccttcgg aggaccaggg ctggggcagc ctctgggccc acatccgggg ccagctccgg
     8281 cgtgtgttca gtgttagcag tgggtcatga tgctctttcc cacccagcct gggatagggg
     8341 cagaggaggc gaggaggccg ttgccgctga tgtttggccg tgaacaggtg ggtgtctgcg
     8401 tgcgtccacg tgcgtgtttt ctgactgaca tgaaatcgac gcccgagtta gcctcacccg
     8461 gtgacctcta gccctgcccg gatggagcgg ggcccacccg gttcagtgtt tctggggagc
     8521 tggacagtgg agtgcaaaag gcttgcagaa cttgaagcct gctccttccc ttgctaccac
     8581 ggcctccttt ccgtttgatt tgtcactgct tcaatcaata acagccgctc cagagtcagt
     8641 agtcaatgaa tatatgacca aatatcacca ggactgttac tcaatgtgtg ccgagccctt
     8701 gcccatgctg ggctcccgtg tatctggaca ctgtaacgtg tgctgtgttt gctccccttc
     8761 cccttccttc tttgcccttt acttgtcttt ctggggtttt tctgtttggg tttggtttgg
     8821 tttttatttc tccttttgtg ttccaaacat gaggttctct ctactggtcc tcttaactgt
     8881 ggtgttgagg cttatatttg tgtaattttt ggtgggtgaa aggaattttg ctaagtaaat
     8941 ctcttctgtg tttgaactga agtctgtatt gtaactatgt ttaaagtaat tgttccagag
     9001 acaaatattt ctagacactt tttctttaca aacaaaagca ttcggaggga gggggatggt
     9061 gactgagatg agaggggaga gctgaacaga tgacccctgc ccagatcagc cagaagccac
     9121 ccaaagcagt ggagcccagg agtcccactc caagccagca agccgaatag ctgatgtgtt
     9181 gccactttcc aagtcactgc aaaaccaggt tttgttccgc ccagtggatt cttgttttgc
     9241 ttcccctccc cccgagatta ttaccaccat cccgtgcttt taaggaaagg caagattgat
     9301 gtttccttga ggggagccag gaggggatgt gtgtgtgcag agctgaagag ctggggagaa
     9361 tggggctggg cccacccaag caggaggctg ggacgctctg ctgtgggcac aggtcaggct
     9421 aatgttggca gatgcagctc ttcctggaca ggccaggtgg tgggcattct ctctccaagg
     9481 tgtgccccgt gggcattact gtttaagaca cttccgtcac atcccacccc atcctccagg
     9541 gctcaacact gtgacatctc tattccccac cctccccttc ccagggcaat aaaatgacca
     9601 tggagggggc ttgcactctc ttggctgtca cccgatcgcc agcaaaactt agatgtgaga
     9661 aaaccccttc ccattccatg gcgaaaacat ctccttagaa aagccattac cctcattagg
     9721 catggttttg ggctcccaaa acacctgaca gcccctccct cctctgagag gcggagagtg
     9781 ctgactgtag tgaccattgc atgccgggtg cagcatctgg aagagctagg cagggtgtct
     9841 gccccctcct gagttgaagt catgctcccc tgtgccagcc cagaggccga gagctatgga
     9901 cagcattgcc agtaacacag gccaccctgt gcagaaggga gctggctcca gcctggaaac
     9961 ctgtctgagg ttgggagagg tgcacttggg gcacagggag aggccgggac acacttagct
    10021 ggagatgtct ctaaaagccc tgtatcgtat tcaccttcag tttttgtgtt ttgggacaat
    10081 tactttagaa aataagtagg tcgttttaaa aacaaaaatt attgattgct tttttgtagt
    10141 gttcagaaaa aaggttcttt gtgtatagcc aaatgactga aagcactgat atatttaaaa
    10201 acaaaaggca atttattaag gaaatttgta ccatttcagt aaacctgtct gaatgtacct
    10261 gtatacgttt caaaaacacc ccccccccac tgaatccctg taacctattt attatataaa
    10321 gagtttgcct tataaattta cataaaaatg tccgtttgtg tcttttgttg taaaaatcaa
    10381 gtgatttttt cataaggttc ttttactatt ggaaaagatg ggcagcacgc agttttattt
    10441 tatttttgta agttttttaa tacatgtgaa agcaaagaat actcagcatg cctttctaag
    10501 tgacgcgttt gcaccttttg ttgggaagta ctgtatcctg tgctgttagc attctcgata
    10561 aatctctctg tgaaagtga
//
