LOCUS       NM_001369391           10930 bp    mRNA    linear   PRI 11-OCT-2024
DEFINITION  Homo sapiens methyl-CpG binding protein 2 (MECP2), transcript
            variant 4, mRNA.
ACCESSION   NM_001369391
VERSION     NM_001369391.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10930)
  AUTHORS   Tomasello,D.L., Barrasa,M.I., Mankus,D., Alarcon,K.I.,
            Lytton-Jean,A.K.R., Liu,X.S. and Jaenisch,R.
  TITLE     Mitochondrial dysfunction and increased reactive oxygen species
            production in MECP2 mutant astrocytes and their impact on neurons
  JOURNAL   Sci Rep 14 (1), 20565 (2024)
   PUBMED   39232000
  REMARK    GeneRIF: Mitochondrial dysfunction and increased reactive oxygen
            species production in MECP2 mutant astrocytes and their impact on
            neurons.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10930)
  AUTHORS   Fichou,Y., Nectoux,J., Bahi-Buisson,N., Rosas-Vargas,H., Girard,B.,
            Chelly,J. and Bienvenu,T.
  TITLE     The first missense mutation causing Rett syndrome specifically
            affecting the MeCP2_e1 isoform
  JOURNAL   Neurogenetics 10 (2), 127-133 (2009)
   PUBMED   19034540
  REMARK    GeneRIF: Data suggest the important involvement of the N-terminus
            in the function of MeCP2 protein, and provide further evidence for
            the major impact of a specific MeCP2e_1 deficiency in the
            development of intellectual processing.
REFERENCE   3  (bases 1 to 10930)
  AUTHORS   Harvey,C.G., Menon,S.D., Stachowiak,B., Noor,A., Proctor,A.,
            Mensah,A.K., Mnatzakanian,G.N., Alfred,S.E., Guo,R., Scherer,S.W.,
            Kennedy,J.L., Roberts,W., Srivastava,A.K., Minassian,B.A. and
            Vincent,J.B.
  TITLE     Sequence variants within exon 1 of MECP2 occur in females with
            mental retardation
  JOURNAL   Am J Med Genet B Neuropsychiatr Genet 144B (3), 355-360 (2007)
   PUBMED   17171659
  REMARK    GeneRIF: polymorphisms within exon 1 of MECP2 occur in females with
            mental retardation.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Erratum:[Am J Med Genet B Neuropsychiatr Genet. 2007 Oct
            5;144(7):969. Srivistava, Anand K [corrected to Srivastava, Anand
            K]]
REFERENCE   4  (bases 1 to 10930)
  AUTHORS   Francke,U.
  TITLE     Mechanisms of disease: neurogenetics of MeCP2 deficiency
  JOURNAL   Nat Clin Pract Neurol 2 (4), 212-221 (2006)
   PUBMED   16932552
  REMARK    GeneRIF: Rett syndrome is caused by heterozygosity for mutations in
            the X-linked gene MECP2, which encodes methyl-CpG binding protein
            2.
            Review article
REFERENCE   5  (bases 1 to 10930)
  AUTHORS   Mnatzakanian,G.N., Lohi,H., Munteanu,I., Alfred,S.E., Yamada,T.,
            MacLeod,P.J., Jones,J.R., Scherer,S.W., Schanen,N.C., Friez,M.J.,
            Vincent,J.B. and Minassian,B.A.
  TITLE     A previously unidentified MECP2 open reading frame defines a new
            protein isoform relevant to Rett syndrome
  JOURNAL   Nat Genet 36 (4), 339-341 (2004)
   PUBMED   15034579
  REMARK    GeneRIF: description of a previously unknown MeCP2 isoform
            Erratum:[Nat Genet. 2004 May;36(5):540]
REFERENCE   6  (bases 1 to 10930)
  AUTHORS   Reichwald,K., Thiesen,J., Wiehe,T., Weitzel,J., Poustka,W.A.,
            Rosenthal,A., Platzer,M., Stratling,W.H. and Kioschis,P.
  TITLE     Comparative sequence analysis of the MECP2-locus in human and mouse
            reveals new transcribed regions
  JOURNAL   Mamm Genome 11 (3), 182-190 (2000)
   PUBMED   10723722
REFERENCE   7  (bases 1 to 10930)
  AUTHORS   Kaur,S. and Christodoulou,J.
  TITLE     MECP2 Disorders
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301670
REFERENCE   8  (bases 1 to 10930)
  AUTHORS   Van Esch,H.
  TITLE     MECP2 Duplication Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301461
REFERENCE   9  (bases 1 to 10930)
  AUTHORS   Klein,C., Lohmann,K., Marras,C. and Munchau,A.
  TITLE     Hereditary Dystonia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301334
REFERENCE   10 (bases 1 to 10930)
  AUTHORS   Lewis,J.D., Meehan,R.R., Henzel,W.J., Maurer-Fogy,I., Jeppesen,P.,
            Klein,F. and Bird,A.
  TITLE     Purification, sequence, and cellular localization of a novel
            chromosomal protein that binds to methylated DNA
  JOURNAL   Cell 69 (6), 905-914 (1992)
   PUBMED   1606614
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC244097.3, AF030876.2 and
            U52112.2.
            
            On Jun 2, 2019 this sequence version replaced NM_001369391.1.
            
            Summary: DNA methylation is the major modification of eukaryotic
            genomes and plays an essential role in mammalian development. Human
            proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of
            nuclear proteins related by the presence in each of a methyl-CpG
            binding domain (MBD). Each of these proteins, with the exception of
            MBD3, is capable of binding specifically to methylated DNA. MECP2,
            MBD1 and MBD2 can also repress transcription from methylated gene
            promoters. In contrast to other MBD family members, MECP2 is
            X-linked and subject to X inactivation. MECP2 is dispensible in
            stem cells, but is essential for embryonic development. MECP2 gene
            mutations are the cause of most cases of Rett syndrome, a
            progressive neurologic developmental disorder and one of the most
            common causes of cognitive disability in females. Alternative
            splicing results in multiple transcript variants encoding different
            isoforms. [provided by RefSeq, Oct 2015].
            
            Transcript Variant: This variant (4) includes exon 2 and some
            additional exons, resulting in the use of a downstream start codon,
            compared to variant 1. The encoded isoform (3) has a shorter
            N-terminus compared to isoform 1. Variants 3-7 all encode the same
            isoform (3).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038196.2578163.1,
                                           SRR7346977.1165955.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 5' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-114               AC244097.3         29110-29223         c
            115-238             AC244097.3         23690-23813         c
            239-362             AF030876.2         49871-49994         c
            363-462             AF030876.2         49215-49314         c
            463-701             AF030876.2         48413-48651         c
            702-1052            AF030876.2         23679-24029         c
            1053-4093           AF030876.2         19882-22922         c
            4094-10930          U52112.2           174507-181343       c
FEATURES             Location/Qualifiers
     source          1..10930
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq28"
     gene            1..10930
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="methyl-CpG binding protein 2"
                     /db_xref="GeneID:4204"
                     /db_xref="HGNC:HGNC:6990"
                     /db_xref="MIM:300005"
     exon            1..114
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     exon            115..238
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     exon            239..362
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     exon            363..462
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     exon            463..701
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     exon            702..1052
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     CDS             955..2136
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="isoform 3 is encoded by transcript variant 4;
                     meCp-2 protein"
                     /codon_start=1
                     /product="methyl-CpG-binding protein 2 isoform 3"
                     /protein_id="NP_001356320.1"
                     /db_xref="GeneID:4204"
                     /db_xref="HGNC:HGNC:6990"
                     /db_xref="MIM:300005"
                     /translation="MYDDPTLPEGWTRKLKQRKSGRSAGKYDVYLINPQGKAFRSKVE
                     LIAYFEKVGDTSLDPNDFDFTVTGRGSPSRREQKPPKKPKSPKAPGTGRGRGRPKGSG
                     TTRPKAATSEGVQVKRVLEKSPGKLLVKMPFQTSPGGKAEGGGATTSTQVMVIKRPGR
                     KRKAEADPQAIPKKRGRKPGSVVAAAAAEAKKKAVKESSIRSVQETVLPIKKRKTRET
                     VSIEVKEVVKPLLVSTLGEKSGKGLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHH
                     HHHSESPKAPVPLLPPLPPPPPEPESSEDPTSPPEPQDLSSSVCKEEKMPRGGSLESD
                     GCPKEPAKTQPAVATAATAAEKYKHRGEGERKDIVSSSMPRPNREEPVDSRTPVTERV
                     S"
     exon            1053..10930
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10908..10913
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="hexamer: GATAAA"
     polyA_site      10930
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="major polyA site"
ORIGIN      
        1 gcgcgcgctc cctcctctcg gagagagggc tgtggtaaaa gccgtccgga aaatggccgc
       61 cgccgccgcc gccgcgccga gcggaggagg aggaggaggc gaggaggaga gactgctcca
      121 taaaaataca gactcaccag ttcctgcttt gatgtgacat gtgactcccc agaatacacc
      181 ttgcttctgt agaccagctc caacaggatt ccatggtagc tgggatgtta gggctcagga
      241 cccaggaggg agaagcagct gccaggggaa gtctcttcgt aggcggaggt caggagtcca
      301 agaggagtga gcagagtcac agaagcctct taaagcctct tcttccccca tcccatcaac
      361 acatctgctg cccaagaact cctttgtgat tgaatttaac cagagttgat ttctgttgct
      421 tgaaactcag gaccttgatg cagtgtgcag ggtctgggca aggaaattca taaaagaaga
      481 gatacagatg gccaatgaag ataggaaaag agttctgcct gctggtaatc aaagagatgc
      541 aaacgagaac aaaaatgatg ccttttcacc taccaaattt gtcaagatta aaaagaaagc
      601 aaagagccag cgtcagctga tgttcatacc tgcacctgct cggtagcttg ctaatgttct
      661 gcctgctcca cacgccaggc cagcctccac ggcgcagcca gggaagaaaa gtcagaagac
      721 caggacctcc agggcctcaa ggacaaaccc ctcaagttta aaaaggtgaa gaaagataag
      781 aaagaagaga aagagggcaa gcatgagccc gtgcagccat cagcccacca ctctgctgag
      841 cccgcagagg caggcaaagc agagacatca gaagggtcag gctccgcccc ggctgtgccg
      901 gaagcttctg cctcccccaa acagcggcgc tccatcatcc gtgaccgggg acccatgtat
      961 gatgacccca ccctgcctga aggctggaca cggaagctta agcaaaggaa atctggccgc
     1021 tctgctggga agtatgatgt gtatttgatc aatccccagg gaaaagcctt tcgctctaaa
     1081 gtggagttga ttgcgtactt cgaaaaggta ggcgacacat ccctggaccc taatgatttt
     1141 gacttcacgg taactgggag agggagcccc tcccggcgag agcagaaacc acctaagaag
     1201 cccaaatctc ccaaagctcc aggaactggc agaggccggg gacgccccaa agggagcggc
     1261 accacgagac ccaaggcggc cacgtcagag ggtgtgcagg tgaaaagggt cctggagaaa
     1321 agtcctggga agctccttgt caagatgcct tttcaaactt cgccaggggg caaggctgag
     1381 gggggtgggg ccaccacatc cacccaggtc atggtgatca aacgccccgg caggaagcga
     1441 aaagctgagg ccgaccctca ggccattccc aagaaacggg gccgaaagcc ggggagtgtg
     1501 gtggcagccg ctgccgccga ggccaaaaag aaagccgtga aggagtcttc tatccgatct
     1561 gtgcaggaga ccgtactccc catcaagaag cgcaagaccc gggagacggt cagcatcgag
     1621 gtcaaggaag tggtgaagcc cctgctggtg tccaccctcg gtgagaagag cgggaaagga
     1681 ctgaagacct gtaagagccc tgggcggaaa agcaaggaga gcagccccaa ggggcgcagc
     1741 agcagcgcct cctcaccccc caagaaggag caccaccacc atcaccacca ctcagagtcc
     1801 ccaaaggccc ccgtgccact gctcccaccc ctgcccccac ctccacctga gcccgagagc
     1861 tccgaggacc ccaccagccc ccctgagccc caggacttga gcagcagcgt ctgcaaagag
     1921 gagaagatgc ccagaggagg ctcactggag agcgacggct gccccaagga gccagctaag
     1981 actcagcccg cggttgccac cgccgccacg gccgcagaaa agtacaaaca ccgaggggag
     2041 ggagagcgca aagacattgt ttcatcctcc atgccaaggc caaacagaga ggagcctgtg
     2101 gacagccgga cgcccgtgac cgagagagtt agctgacttt acacggagcg gattgcaaag
     2161 caaaccaaca agaataaagg cagctgttgt ctcttctcct tatgggtagg gctctgacaa
     2221 agcttcccga ttaactgaaa taaaaaatat ttttttttct ttcagtaaac ttagagtttc
     2281 gtggcttcag ggtgggagta gttggagcat tggggatgtt tttcttaccg acaagcacag
     2341 tcaggttgaa gacctaacca gggccagaag tagctttgca cttttctaaa ctaggctcct
     2401 tcaacaaggc ttgctgcaga tactactgac cagacaagct gttgaccagg cacctcccct
     2461 cccgcccaaa cctttccccc atgtggtcgt tagagacaga gcgacagagc agttgagagg
     2521 acactcccgt tttcggtgcc atcagtgccc cgtctacagc tcccccagct ccccccacct
     2581 cccccactcc caaccacgtt gggacaggga ggtgtgaggc aggagagaca gttggattct
     2641 ttagagaaga tggatatgac cagtggctat ggcctgtgcg atcccacccg tggtggctca
     2701 agtctggccc cacaccagcc ccaatccaaa actggcaagg acgcttcaca ggacaggaaa
     2761 gtggcacctg tctgctccag ctctggcatg gctaggaggg gggagtccct tgaactactg
     2821 ggtgtagact ggcctgaacc acaggagagg atggcccagg gtgaggtggc atggtccatt
     2881 ctcaagggac gtcctccaac gggtggcgct agaggccatg gaggcagtag gacaaggtgc
     2941 aggcaggctg gcctggggtc aggccgggca gagcacagcg gggtgagagg gattcctaat
     3001 cactcagagc agtctgtgac ttagtggaca ggggaggggg caaaggggga ggagaagaaa
     3061 atgttcttcc agttactttc caattctcct ttagggacag cttagaatta tttgcactat
     3121 tgagtcttca tgttcccact tcaaaacaaa cagatgctct gagagcaaac tggcttgaat
     3181 tggtgacatt tagtccctca agccaccaga tgtgacagtg ttgagaacta cctggatttg
     3241 tatatatacc tgcgcttgtt ttaaagtggg ctcagcacat agggttccca cgaagctccg
     3301 aaactctaag tgtttgctgc aattttataa ggacttcctg attggtttct cttctcccct
     3361 tccatttctg ccttttgttc atttcatcct ttcacttctt tcccttcctc catcctcctc
     3421 cttcctagtt catcccttct cttccaggca gccgcggtgc ccaaccacac ttgtcggctc
     3481 cagtccccag aactctgcct gccctttgtc ctcctgctgc cagtaccagc cccaccctgt
     3541 tttgagccct gaggaggcct tgggctctgc tgagtccgac ctggcctgtc tgtgaagagc
     3601 aagagagcag caaggtcttg ctctcctagg tagccccctc ttccctggta agaaaaagca
     3661 aaaggcattt cccaccctga acaacgagcc ttttcaccct tctactctag agaagtggac
     3721 tggaggagct gggcccgatt tggtagttga ggaaagcaca gaggcctcct gtggcctgcc
     3781 agtcatcgag tggcccaaca ggggctccat gccagccgac cttgacctca ctcagaagtc
     3841 cagagtctag cgtagtgcag cagggcagta gcggtaccaa tgcagaactc ccaagacccg
     3901 agctgggacc agtacctggg tccccagccc ttcctctgct cccccttttc cctcggagtt
     3961 cttcttgaat ggcaatgttt tgcttttgct cgatgcagac agggggccag aacaccacac
     4021 atttcactgt ctgtctggtc catagctgtg gtgtaggggc ttagaggcat gggcttgctg
     4081 tgggttttta attgatcagt tttcatgtgg gatcccatct ttttaacctc tgttcaggaa
     4141 gtccttatct agctgcatat cttcatcata ttggtatatc cttttctgtg tttacagaga
     4201 tgtctcttat atctaaatct gtccaactga gaagtacctt atcaaagtag caaatgagac
     4261 agcagtctta tgcttccaga aacacccaca ggcatgtccc atgtgagctg ctgccatgaa
     4321 ctgtcaagtg tgtgttgtct tgtgtatttc agttattgtc cctggcttcc ttactatggt
     4381 gtaatcatga aggagtgaaa catcatagaa actgtctagc acttccttgc cagtctttag
     4441 tgatcaggaa ccatagttga cagttccaat cagtagctta agaaaaaacc gtgtttgtct
     4501 cttctggaat ggttagaagt gagggagttt gccccgttct gtttgtagag tctcatagtt
     4561 ggactttcta gcatatatgt gtccatttcc ttatgctgta aaagcaagtc ctgcaaccaa
     4621 actcccatca gcccaatccc tgatccctga tcccttccac ctgctctgct gatgaccccc
     4681 ccagcttcac ttctgactct tccccaggaa gggaaggggg gtcagaagag agggtgagtc
     4741 ctccagaact cttcctccaa ggacagaagg ctcctgcccc catagtggcc tcgaactcct
     4801 ggcactacca aaggacactt atccacgaga gcgcagcatc cgaccaggtt gtcactgaga
     4861 agatgtttat tttggtcagt tgggttttta tgtattatac ttagtcaaat gtaatgtggc
     4921 ttctggaatc attgtccaga gctgcttccc cgtcacctgg gcgtcatctg gtcctggtaa
     4981 gaggagtgcg tggcccacca ggcccccctg tcacccatga cagttcattc agggccgatg
     5041 gggcagtcgt ggttgggaac acagcatttc aagcgtcact ttatttcatt cgggccccac
     5101 ctgcagctcc ctcaaagagg cagttgccca gcctctttcc cttccagttt attccagagc
     5161 tgccagtggg gcctgaggct ccttagggtt ttctctctat ttcccccttt cttcctcatt
     5221 ccctcgtctt tcccaaaggc atcacgagtc agtcgccttt cagcaggcag ccttggcggt
     5281 ttatcgccct ggcaggcagg ggccctgcag ctctcatgct gcccctgcct tggggtcagg
     5341 ttgacaggag gttggaggga aagccttaag ctgcaggatt ctcaccagct gtgtccggcc
     5401 cagttttggg gtgtgacctc aatttcaatt ttgtctgtac ttgaacatta tgaagatggg
     5461 ggcctctttc agtgaatttg tgaacagcag aattgaccga cagctttcca gtacccatgg
     5521 ggctaggtca ttaaggccac atccacagtc tcccccaccc ttgttccagt tgttagttac
     5581 tacctcctct cctgacaata ctgtatgtcg tcgagctccc cccaggtcta cccctcccgg
     5641 ccctgcctgc tggtgggctt gtcatagcca gtgggattgc cggtcttgac agctcagtga
     5701 gctggagata cttggtcaca gccaggcgct agcacagctc ccttctgttg atgctgtatt
     5761 cccatatcaa aagacacagg ggacacccag aaacgccaca tcccccaatc catcagtgcc
     5821 aaactagcca acggccccag cttctcagct cgctggatgg cggaagctgc tactcgtgag
     5881 cgccagtgcg ggtgcagaca atcttctgtt gggtggcatc attccaggcc cgaagcatga
     5941 acagtgcacc tgggacaggg agcagcccca aattgtcacc tgcttctctg cccagctttt
     6001 cattgctgtg acagtgatgg cgaaagaggg taataaccag acacaaactg ccaagttggg
     6061 tggagaaagg agtttcttta gctgacagaa tctctgaatt ttaaatcact tagtaagcgg
     6121 ctcaagccca ggagggagca gagggatacg agcggagtcc cctgcgcggg accatctgga
     6181 attggtttag cccaagtgga gcctgacagc cagaactctg tgtcccccgt ctaaccacag
     6241 ctccttttcc agagcattcc agtcaggctc tctgggctga ctgggccagg ggaggttaca
     6301 ggtaccagtt ctttaagaag atctttgggc atatacattt ttagcctgtg tcattgcccc
     6361 aaatggattc ctgtttcaag ttcacacctg cagattctag gacctgtgtc ctagacttca
     6421 gggagtcagc tgtttctaga gttcctacca tggagtgggt ctggaggacc tgcccggtgg
     6481 gggggcagag ccctgctccc tccgggtctt cctactcttc tctctgctct gacgggattt
     6541 gttgattctc tccattttgg tgtctttctc ttttagatat tgtatcaatc tttagaaaag
     6601 gcatagtcta cttgttataa atcgttagga tactgcctcc cccagggtct aaaattacat
     6661 attagagggg aaaagctgaa cactgaagtc agttctcaac aatttagaag gaaaacctag
     6721 aaaacatttg gcagaaaatt acatttcgat gtttttgaat gaatacgagc aagcttttac
     6781 aacagtgctg atctaaaaat acttagcact tggcctgaga tgcctggtga gcattacagg
     6841 caaggggaat ctggaggtag ccgacctgag gacatggctt ctgaacctgt cttttgggag
     6901 tggtatggaa ggtggagcgt tcaccagtga cctggaaggc ccagcaccac cctccttccc
     6961 actcttctca tcttgacaga gcctgcccca gcgctgacgt gtcaggaaaa cacccaggga
     7021 actaggaagg cacttctgcc tgaggggcag cctgccttgc ccactcctgc tctgctcgcc
     7081 tcggatcagc tgagccttct gagctggcct ctcactgcct ccccaaggcc ccctgcctgc
     7141 cctgtcagga ggcagaagga agcaggtgtg agggcagtgc aaggagggag cacaaccccc
     7201 agctcccgct ccgggctccg acttgtgcac aggcagagcc cagaccctgg aggaaatcct
     7261 acctttgaat tcaagaacat ttggggaatt tggaaatctc tttgccccca aacccccatt
     7321 ctgtcctacc tttaatcagg tcctgctcag cagtgagagc agatgaggtg aaaaggccaa
     7381 gaggtttggc tcctgcccac tgatagcccc tctccccgca gtgtttgtgt gtcaagtggc
     7441 aaagctgttc ttcctggtga ccctgattat atccagtaac acatagactg tgcgcatagg
     7501 cctgctttgt ctcctctatc ctgggctttt gttttgcttt ttagttttgc ttttagtttt
     7561 tctgtccctt ttatttaacg caccgactag acacacaaag cagttgaatt tttatatata
     7621 tatctgtata ttgcacaatt ataaactcat tttgcttgtg gctccacaca cacaaaaaaa
     7681 gacctgttaa aattatacct gttgcttaat tacaatattt ctgataacca tagcatagga
     7741 caagggaaaa taaaaaaaga aaaaaaagaa aaaaaaacga caaatctgtc tgctggtcac
     7801 ttcttctgtc caagcagatt cgtggtcttt tcctcgcttc tttcaagggc tttcctgtgc
     7861 caggtgaagg aggctccagg cagcacccag gttttgcact cttgtttctc ccgtgcttgt
     7921 gaaagaggtc ccaaggttct gggtgcagga gcgctccctt gacctgctga agtccggaac
     7981 gtagtcggca cagcctggtc gccttccacc tctgggagct ggagtccact ggggtggcct
     8041 gactccccca gtccccttcc cgtgacctgg tcagggtgag cccatgtgga gtcagcctcg
     8101 caggcctccc tgccagtagg gtccgagtgt gtttcatcct tcccactctg tcgagcctgg
     8161 gggctggagc ggagacggga ggcctggcct gtctcggaac ctgtgagctg caccaggtag
     8221 aacgccaggg accccagaat catgtgcgtc agtccaaggg gtcccctcca ggagtagtga
     8281 agactccaga aatgtccctt tcttctcccc catcctacga gtaattgcat ttgcttttgt
     8341 aattcttaat gagcaatatc tgctagagag tttagctgta acagttcttt ttgatcatct
     8401 ttttttaata attagaaaca ccaaaaaaat ccagaaactt gttcttccaa agcagagagc
     8461 attataatca ccagggccaa aagcttccct ccctgctgtc attgcttctt ctgaggcctg
     8521 aatccaaaag aaaaacagcc ataggccctt tcagtggccg ggctacccgt gagcccttcg
     8581 gaggaccagg gctggggcag cctctgggcc cacatccggg gccagctccg gcgtgtgttc
     8641 agtgttagca gtgggtcatg atgctctttc ccacccagcc tgggataggg gcagaggagg
     8701 cgaggaggcc gttgccgctg atgtttggcc gtgaacaggt gggtgtctgc gtgcgtccac
     8761 gtgcgtgttt tctgactgac atgaaatcga cgcccgagtt agcctcaccc ggtgacctct
     8821 agccctgccc ggatggagcg gggcccaccc ggttcagtgt ttctggggag ctggacagtg
     8881 gagtgcaaaa ggcttgcaga acttgaagcc tgctccttcc cttgctacca cggcctcctt
     8941 tccgtttgat ttgtcactgc ttcaatcaat aacagccgct ccagagtcag tagtcaatga
     9001 atatatgacc aaatatcacc aggactgtta ctcaatgtgt gccgagccct tgcccatgct
     9061 gggctcccgt gtatctggac actgtaacgt gtgctgtgtt tgctcccctt ccccttcctt
     9121 ctttgccctt tacttgtctt tctggggttt ttctgtttgg gtttggtttg gtttttattt
     9181 ctccttttgt gttccaaaca tgaggttctc tctactggtc ctcttaactg tggtgttgag
     9241 gcttatattt gtgtaatttt tggtgggtga aaggaatttt gctaagtaaa tctcttctgt
     9301 gtttgaactg aagtctgtat tgtaactatg tttaaagtaa ttgttccaga gacaaatatt
     9361 tctagacact ttttctttac aaacaaaagc attcggaggg agggggatgg tgactgagat
     9421 gagaggggag agctgaacag atgacccctg cccagatcag ccagaagcca cccaaagcag
     9481 tggagcccag gagtcccact ccaagccagc aagccgaata gctgatgtgt tgccactttc
     9541 caagtcactg caaaaccagg ttttgttccg cccagtggat tcttgttttg cttcccctcc
     9601 ccccgagatt attaccacca tcccgtgctt ttaaggaaag gcaagattga tgtttccttg
     9661 aggggagcca ggaggggatg tgtgtgtgca gagctgaaga gctggggaga atggggctgg
     9721 gcccacccaa gcaggaggct gggacgctct gctgtgggca caggtcaggc taatgttggc
     9781 agatgcagct cttcctggac aggccaggtg gtgggcattc tctctccaag gtgtgccccg
     9841 tgggcattac tgtttaagac acttccgtca catcccaccc catcctccag ggctcaacac
     9901 tgtgacatct ctattcccca ccctcccctt cccagggcaa taaaatgacc atggaggggg
     9961 cttgcactct cttggctgtc acccgatcgc cagcaaaact tagatgtgag aaaacccctt
    10021 cccattccat ggcgaaaaca tctccttaga aaagccatta ccctcattag gcatggtttt
    10081 gggctcccaa aacacctgac agcccctccc tcctctgaga ggcggagagt gctgactgta
    10141 gtgaccattg catgccgggt gcagcatctg gaagagctag gcagggtgtc tgccccctcc
    10201 tgagttgaag tcatgctccc ctgtgccagc ccagaggccg agagctatgg acagcattgc
    10261 cagtaacaca ggccaccctg tgcagaaggg agctggctcc agcctggaaa cctgtctgag
    10321 gttgggagag gtgcacttgg ggcacaggga gaggccggga cacacttagc tggagatgtc
    10381 tctaaaagcc ctgtatcgta ttcaccttca gtttttgtgt tttgggacaa ttactttaga
    10441 aaataagtag gtcgttttaa aaacaaaaat tattgattgc ttttttgtag tgttcagaaa
    10501 aaaggttctt tgtgtatagc caaatgactg aaagcactga tatatttaaa aacaaaaggc
    10561 aatttattaa ggaaatttgt accatttcag taaacctgtc tgaatgtacc tgtatacgtt
    10621 tcaaaaacac ccccccccca ctgaatccct gtaacctatt tattatataa agagtttgcc
    10681 ttataaattt acataaaaat gtccgtttgt gtcttttgtt gtaaaaatca agtgattttt
    10741 tcataaggtt cttttactat tggaaaagat gggcagcacg cagttttatt ttatttttgt
    10801 aagtttttta atacatgtga aagcaaagaa tactcagcat gcctttctaa gtgacgcgtt
    10861 tgcacctttt gttgggaagt actgtatcct gtgctgttag cattctcgat aaatctctct
    10921 gtgaaagtga
//
