LOCUS       NM_001110792           10343 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens methyl-CpG binding protein 2 (MECP2), transcript
            variant 2, mRNA.
ACCESSION   NM_001110792
VERSION     NM_001110792.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10343)
  AUTHORS   Tomasello,D.L., Barrasa,M.I., Mankus,D., Alarcon,K.I.,
            Lytton-Jean,A.K.R., Liu,X.S. and Jaenisch,R.
  TITLE     Mitochondrial dysfunction and increased reactive oxygen species
            production in MECP2 mutant astrocytes and their impact on neurons
  JOURNAL   Sci Rep 14 (1), 20565 (2024)
   PUBMED   39232000
  REMARK    GeneRIF: Mitochondrial dysfunction and increased reactive oxygen
            species production in MECP2 mutant astrocytes and their impact on
            neurons.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10343)
  AUTHORS   Fichou,Y., Nectoux,J., Bahi-Buisson,N., Rosas-Vargas,H., Girard,B.,
            Chelly,J. and Bienvenu,T.
  TITLE     The first missense mutation causing Rett syndrome specifically
            affecting the MeCP2_e1 isoform
  JOURNAL   Neurogenetics 10 (2), 127-133 (2009)
   PUBMED   19034540
  REMARK    GeneRIF: Data suggest the important involvement of the N-terminus
            in the function of MeCP2 protein, and provide further evidence for
            the major impact of a specific MeCP2e_1 deficiency in the
            development of intellectual processing.
REFERENCE   3  (bases 1 to 10343)
  AUTHORS   Harvey,C.G., Menon,S.D., Stachowiak,B., Noor,A., Proctor,A.,
            Mensah,A.K., Mnatzakanian,G.N., Alfred,S.E., Guo,R., Scherer,S.W.,
            Kennedy,J.L., Roberts,W., Srivastava,A.K., Minassian,B.A. and
            Vincent,J.B.
  TITLE     Sequence variants within exon 1 of MECP2 occur in females with
            mental retardation
  JOURNAL   Am J Med Genet B Neuropsychiatr Genet 144B (3), 355-360 (2007)
   PUBMED   17171659
  REMARK    GeneRIF: polymorphisms within exon 1 of MECP2 occur in females with
            mental retardation.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Erratum:[Am J Med Genet B Neuropsychiatr Genet. 2007 Oct
            5;144(7):969. Srivistava, Anand K [corrected to Srivastava, Anand
            K]]
REFERENCE   4  (bases 1 to 10343)
  AUTHORS   Francke,U.
  TITLE     Mechanisms of disease: neurogenetics of MeCP2 deficiency
  JOURNAL   Nat Clin Pract Neurol 2 (4), 212-221 (2006)
   PUBMED   16932552
  REMARK    GeneRIF: Rett syndrome is caused by heterozygosity for mutations in
            the X-linked gene MECP2, which encodes methyl-CpG binding protein
            2.
            Review article
REFERENCE   5  (bases 1 to 10343)
  AUTHORS   Mnatzakanian,G.N., Lohi,H., Munteanu,I., Alfred,S.E., Yamada,T.,
            MacLeod,P.J., Jones,J.R., Scherer,S.W., Schanen,N.C., Friez,M.J.,
            Vincent,J.B. and Minassian,B.A.
  TITLE     A previously unidentified MECP2 open reading frame defines a new
            protein isoform relevant to Rett syndrome
  JOURNAL   Nat Genet 36 (4), 339-341 (2004)
   PUBMED   15034579
  REMARK    GeneRIF: description of a previously unknown MeCP2 isoform
            Erratum:[Nat Genet. 2004 May;36(5):540]
REFERENCE   6  (bases 1 to 10343)
  AUTHORS   Reichwald,K., Thiesen,J., Wiehe,T., Weitzel,J., Poustka,W.A.,
            Rosenthal,A., Platzer,M., Stratling,W.H. and Kioschis,P.
  TITLE     Comparative sequence analysis of the MECP2-locus in human and mouse
            reveals new transcribed regions
  JOURNAL   Mamm Genome 11 (3), 182-190 (2000)
   PUBMED   10723722
REFERENCE   7  (bases 1 to 10343)
  AUTHORS   Kaur,S. and Christodoulou,J.
  TITLE     MECP2 Disorders
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301670
REFERENCE   8  (bases 1 to 10343)
  AUTHORS   Van Esch,H.
  TITLE     MECP2 Duplication Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301461
REFERENCE   9  (bases 1 to 10343)
  AUTHORS   Klein,C., Lohmann,K., Marras,C. and Munchau,A.
  TITLE     Hereditary Dystonia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301334
REFERENCE   10 (bases 1 to 10343)
  AUTHORS   Lewis,J.D., Meehan,R.R., Henzel,W.J., Maurer-Fogy,I., Jeppesen,P.,
            Klein,F. and Bird,A.
  TITLE     Purification, sequence, and cellular localization of a novel
            chromosomal protein that binds to methylated DNA
  JOURNAL   Cell 69 (6), 905-914 (1992)
   PUBMED   1606614
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BX538060.1, BC011612.1,
            AF030876.2 and U52112.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001110792.1.
            
            Summary: DNA methylation is the major modification of eukaryotic
            genomes and plays an essential role in mammalian development. Human
            proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of
            nuclear proteins related by the presence in each of a methyl-CpG
            binding domain (MBD). Each of these proteins, with the exception of
            MBD3, is capable of binding specifically to methylated DNA. MECP2,
            MBD1 and MBD2 can also repress transcription from methylated gene
            promoters. In contrast to other MBD family members, MECP2 is
            X-linked and subject to X inactivation. MECP2 is dispensible in
            stem cells, but is essential for embryonic development. MECP2 gene
            mutations are the cause of most cases of Rett syndrome, a
            progressive neurologic developmental disorder and one of the most
            common causes of cognitive disability in females. Alternative
            splicing results in multiple transcript variants encoding different
            isoforms. [provided by RefSeq, Oct 2015].
            
            Transcript Variant: This variant (2), also known as MECP2B, lacks
            exon 2. Translation is reported to initiate in the first exon
            resulting in a protein isoform (2) with a distinct N-terminus. This
            transcript is reported to be abundant in the central nervous system
            (PMID: 15034579, 17171659).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX538060.1, SRR7410570.694577.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2467150 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000453960.7/ ENSP00000395535.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-480               BX538060.1         5-484
            481-1682            BC011612.1         477-1678
            1683-3506           AF030876.2         19882-21705         c
            3507-10343          U52112.2           174507-181343       c
FEATURES             Location/Qualifiers
     source          1..10343
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq28"
     gene            1..10343
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="methyl-CpG binding protein 2"
                     /db_xref="GeneID:4204"
                     /db_xref="HGNC:HGNC:6990"
                     /db_xref="MIM:300005"
     exon            1..114
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     CDS             53..1549
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="isoform 2 is encoded by transcript variant 2;
                     meCp-2 protein"
                     /codon_start=1
                     /product="methyl-CpG-binding protein 2 isoform 2"
                     /protein_id="NP_001104262.1"
                     /db_xref="CCDS:CCDS48193.1"
                     /db_xref="GeneID:4204"
                     /db_xref="HGNC:HGNC:6990"
                     /db_xref="MIM:300005"
                     /translation="MAAAAAAAPSGGGGGGEEERLEEKSEDQDLQGLKDKPLKFKKVK
                     KDKKEEKEGKHEPVQPSAHHSAEPAEAGKAETSEGSGSAPAVPEASASPKQRRSIIRD
                     RGPMYDDPTLPEGWTRKLKQRKSGRSAGKYDVYLINPQGKAFRSKVELIAYFEKVGDT
                     SLDPNDFDFTVTGRGSPSRREQKPPKKPKSPKAPGTGRGRGRPKGSGTTRPKAATSEG
                     VQVKRVLEKSPGKLLVKMPFQTSPGGKAEGGGATTSTQVMVIKRPGRKRKAEADPQAI
                     PKKRGRKPGSVVAAAAAEAKKKAVKESSIRSVQETVLPIKKRKTRETVSIEVKEVVKP
                     LLVSTLGEKSGKGLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHHHHHSESPKAPV
                     PLLPPLPPPPPEPESSEDPTSPPEPQDLSSSVCKEEKMPRGGSLESDGCPKEPAKTQP
                     AVATAATAAEKYKHRGEGERKDIVSSSMPRPNREEPVDSRTPVTERVS"
     exon            115..465
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     exon            466..10343
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10321..10326
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="hexamer: GATAAA"
     polyA_site      10343
                     /gene="MECP2"
                     /gene_synonym="AUTSX3; MRX16; MRX79; MRXS13; MRXSL; PPMX;
                     RS; RTS; RTT"
                     /note="major polyA site"
ORIGIN      
        1 gcgcgcgctc cctcctctcg gagagagggc tgtggtaaaa gccgtccgga aaatggccgc
       61 cgccgccgcc gccgcgccga gcggaggagg aggaggaggc gaggaggaga gactggaaga
      121 aaagtcagaa gaccaggacc tccagggcct caaggacaaa cccctcaagt ttaaaaaggt
      181 gaagaaagat aagaaagaag agaaagaggg caagcatgag cccgtgcagc catcagccca
      241 ccactctgct gagcccgcag aggcaggcaa agcagagaca tcagaagggt caggctccgc
      301 cccggctgtg ccggaagctt ctgcctcccc caaacagcgg cgctccatca tccgtgaccg
      361 gggacccatg tatgatgacc ccaccctgcc tgaaggctgg acacggaagc ttaagcaaag
      421 gaaatctggc cgctctgctg ggaagtatga tgtgtatttg atcaatcccc agggaaaagc
      481 ctttcgctct aaagtggagt tgattgcgta cttcgaaaag gtaggcgaca catccctgga
      541 ccctaatgat tttgacttca cggtaactgg gagagggagc ccctcccggc gagagcagaa
      601 accacctaag aagcccaaat ctcccaaagc tccaggaact ggcagaggcc ggggacgccc
      661 caaagggagc ggcaccacga gacccaaggc ggccacgtca gagggtgtgc aggtgaaaag
      721 ggtcctggag aaaagtcctg ggaagctcct tgtcaagatg ccttttcaaa cttcgccagg
      781 gggcaaggct gaggggggtg gggccaccac atccacccag gtcatggtga tcaaacgccc
      841 cggcaggaag cgaaaagctg aggccgaccc tcaggccatt cccaagaaac ggggccgaaa
      901 gccggggagt gtggtggcag ccgctgccgc cgaggccaaa aagaaagccg tgaaggagtc
      961 ttctatccga tctgtgcagg agaccgtact ccccatcaag aagcgcaaga cccgggagac
     1021 ggtcagcatc gaggtcaagg aagtggtgaa gcccctgctg gtgtccaccc tcggtgagaa
     1081 gagcgggaaa ggactgaaga cctgtaagag ccctgggcgg aaaagcaagg agagcagccc
     1141 caaggggcgc agcagcagcg cctcctcacc ccccaagaag gagcaccacc accatcacca
     1201 ccactcagag tccccaaagg cccccgtgcc actgctccca cccctgcccc cacctccacc
     1261 tgagcccgag agctccgagg accccaccag cccccctgag ccccaggact tgagcagcag
     1321 cgtctgcaaa gaggagaaga tgcccagagg aggctcactg gagagcgacg gctgccccaa
     1381 ggagccagct aagactcagc ccgcggttgc caccgccgcc acggccgcag aaaagtacaa
     1441 acaccgaggg gagggagagc gcaaagacat tgtttcatcc tccatgccaa ggccaaacag
     1501 agaggagcct gtggacagcc ggacgcccgt gaccgagaga gttagctgac tttacacgga
     1561 gcggattgca aagcaaacca acaagaataa aggcagctgt tgtctcttct ccttatgggt
     1621 agggctctga caaagcttcc cgattaactg aaataaaaaa tatttttttt tctttcagta
     1681 aacttagagt ttcgtggctt cagggtggga gtagttggag cattggggat gtttttctta
     1741 ccgacaagca cagtcaggtt gaagacctaa ccagggccag aagtagcttt gcacttttct
     1801 aaactaggct ccttcaacaa ggcttgctgc agatactact gaccagacaa gctgttgacc
     1861 aggcacctcc cctcccgccc aaacctttcc cccatgtggt cgttagagac agagcgacag
     1921 agcagttgag aggacactcc cgttttcggt gccatcagtg ccccgtctac agctccccca
     1981 gctcccccca cctcccccac tcccaaccac gttgggacag ggaggtgtga ggcaggagag
     2041 acagttggat tctttagaga agatggatat gaccagtggc tatggcctgt gcgatcccac
     2101 ccgtggtggc tcaagtctgg ccccacacca gccccaatcc aaaactggca aggacgcttc
     2161 acaggacagg aaagtggcac ctgtctgctc cagctctggc atggctagga ggggggagtc
     2221 ccttgaacta ctgggtgtag actggcctga accacaggag aggatggccc agggtgaggt
     2281 ggcatggtcc attctcaagg gacgtcctcc aacgggtggc gctagaggcc atggaggcag
     2341 taggacaagg tgcaggcagg ctggcctggg gtcaggccgg gcagagcaca gcggggtgag
     2401 agggattcct aatcactcag agcagtctgt gacttagtgg acaggggagg gggcaaaggg
     2461 ggaggagaag aaaatgttct tccagttact ttccaattct cctttaggga cagcttagaa
     2521 ttatttgcac tattgagtct tcatgttccc acttcaaaac aaacagatgc tctgagagca
     2581 aactggcttg aattggtgac atttagtccc tcaagccacc agatgtgaca gtgttgagaa
     2641 ctacctggat ttgtatatat acctgcgctt gttttaaagt gggctcagca catagggttc
     2701 ccacgaagct ccgaaactct aagtgtttgc tgcaatttta taaggacttc ctgattggtt
     2761 tctcttctcc ccttccattt ctgccttttg ttcatttcat cctttcactt ctttcccttc
     2821 ctccatcctc ctccttccta gttcatccct tctcttccag gcagccgcgg tgcccaacca
     2881 cacttgtcgg ctccagtccc cagaactctg cctgcccttt gtcctcctgc tgccagtacc
     2941 agccccaccc tgttttgagc cctgaggagg ccttgggctc tgctgagtcc gacctggcct
     3001 gtctgtgaag agcaagagag cagcaaggtc ttgctctcct aggtagcccc ctcttccctg
     3061 gtaagaaaaa gcaaaaggca tttcccaccc tgaacaacga gccttttcac ccttctactc
     3121 tagagaagtg gactggagga gctgggcccg atttggtagt tgaggaaagc acagaggcct
     3181 cctgtggcct gccagtcatc gagtggccca acaggggctc catgccagcc gaccttgacc
     3241 tcactcagaa gtccagagtc tagcgtagtg cagcagggca gtagcggtac caatgcagaa
     3301 ctcccaagac ccgagctggg accagtacct gggtccccag cccttcctct gctccccctt
     3361 ttccctcgga gttcttcttg aatggcaatg ttttgctttt gctcgatgca gacagggggc
     3421 cagaacacca cacatttcac tgtctgtctg gtccatagct gtggtgtagg ggcttagagg
     3481 catgggcttg ctgtgggttt ttaattgatc agttttcatg tgggatccca tctttttaac
     3541 ctctgttcag gaagtcctta tctagctgca tatcttcatc atattggtat atccttttct
     3601 gtgtttacag agatgtctct tatatctaaa tctgtccaac tgagaagtac cttatcaaag
     3661 tagcaaatga gacagcagtc ttatgcttcc agaaacaccc acaggcatgt cccatgtgag
     3721 ctgctgccat gaactgtcaa gtgtgtgttg tcttgtgtat ttcagttatt gtccctggct
     3781 tccttactat ggtgtaatca tgaaggagtg aaacatcata gaaactgtct agcacttcct
     3841 tgccagtctt tagtgatcag gaaccatagt tgacagttcc aatcagtagc ttaagaaaaa
     3901 accgtgtttg tctcttctgg aatggttaga agtgagggag tttgccccgt tctgtttgta
     3961 gagtctcata gttggacttt ctagcatata tgtgtccatt tccttatgct gtaaaagcaa
     4021 gtcctgcaac caaactccca tcagcccaat ccctgatccc tgatcccttc cacctgctct
     4081 gctgatgacc cccccagctt cacttctgac tcttccccag gaagggaagg ggggtcagaa
     4141 gagagggtga gtcctccaga actcttcctc caaggacaga aggctcctgc ccccatagtg
     4201 gcctcgaact cctggcacta ccaaaggaca cttatccacg agagcgcagc atccgaccag
     4261 gttgtcactg agaagatgtt tattttggtc agttgggttt ttatgtatta tacttagtca
     4321 aatgtaatgt ggcttctgga atcattgtcc agagctgctt ccccgtcacc tgggcgtcat
     4381 ctggtcctgg taagaggagt gcgtggccca ccaggccccc ctgtcaccca tgacagttca
     4441 ttcagggccg atggggcagt cgtggttggg aacacagcat ttcaagcgtc actttatttc
     4501 attcgggccc cacctgcagc tccctcaaag aggcagttgc ccagcctctt tcccttccag
     4561 tttattccag agctgccagt ggggcctgag gctccttagg gttttctctc tatttccccc
     4621 tttcttcctc attccctcgt ctttcccaaa ggcatcacga gtcagtcgcc tttcagcagg
     4681 cagccttggc ggtttatcgc cctggcaggc aggggccctg cagctctcat gctgcccctg
     4741 ccttggggtc aggttgacag gaggttggag ggaaagcctt aagctgcagg attctcacca
     4801 gctgtgtccg gcccagtttt ggggtgtgac ctcaatttca attttgtctg tacttgaaca
     4861 ttatgaagat gggggcctct ttcagtgaat ttgtgaacag cagaattgac cgacagcttt
     4921 ccagtaccca tggggctagg tcattaaggc cacatccaca gtctccccca cccttgttcc
     4981 agttgttagt tactacctcc tctcctgaca atactgtatg tcgtcgagct ccccccaggt
     5041 ctacccctcc cggccctgcc tgctggtggg cttgtcatag ccagtgggat tgccggtctt
     5101 gacagctcag tgagctggag atacttggtc acagccaggc gctagcacag ctcccttctg
     5161 ttgatgctgt attcccatat caaaagacac aggggacacc cagaaacgcc acatccccca
     5221 atccatcagt gccaaactag ccaacggccc cagcttctca gctcgctgga tggcggaagc
     5281 tgctactcgt gagcgccagt gcgggtgcag acaatcttct gttgggtggc atcattccag
     5341 gcccgaagca tgaacagtgc acctgggaca gggagcagcc ccaaattgtc acctgcttct
     5401 ctgcccagct tttcattgct gtgacagtga tggcgaaaga gggtaataac cagacacaaa
     5461 ctgccaagtt gggtggagaa aggagtttct ttagctgaca gaatctctga attttaaatc
     5521 acttagtaag cggctcaagc ccaggaggga gcagagggat acgagcggag tcccctgcgc
     5581 gggaccatct ggaattggtt tagcccaagt ggagcctgac agccagaact ctgtgtcccc
     5641 cgtctaacca cagctccttt tccagagcat tccagtcagg ctctctgggc tgactgggcc
     5701 aggggaggtt acaggtacca gttctttaag aagatctttg ggcatataca tttttagcct
     5761 gtgtcattgc cccaaatgga ttcctgtttc aagttcacac ctgcagattc taggacctgt
     5821 gtcctagact tcagggagtc agctgtttct agagttccta ccatggagtg ggtctggagg
     5881 acctgcccgg tgggggggca gagccctgct ccctccgggt cttcctactc ttctctctgc
     5941 tctgacggga tttgttgatt ctctccattt tggtgtcttt ctcttttaga tattgtatca
     6001 atctttagaa aaggcatagt ctacttgtta taaatcgtta ggatactgcc tcccccaggg
     6061 tctaaaatta catattagag gggaaaagct gaacactgaa gtcagttctc aacaatttag
     6121 aaggaaaacc tagaaaacat ttggcagaaa attacatttc gatgtttttg aatgaatacg
     6181 agcaagcttt tacaacagtg ctgatctaaa aatacttagc acttggcctg agatgcctgg
     6241 tgagcattac aggcaagggg aatctggagg tagccgacct gaggacatgg cttctgaacc
     6301 tgtcttttgg gagtggtatg gaaggtggag cgttcaccag tgacctggaa ggcccagcac
     6361 caccctcctt cccactcttc tcatcttgac agagcctgcc ccagcgctga cgtgtcagga
     6421 aaacacccag ggaactagga aggcacttct gcctgagggg cagcctgcct tgcccactcc
     6481 tgctctgctc gcctcggatc agctgagcct tctgagctgg cctctcactg cctccccaag
     6541 gccccctgcc tgccctgtca ggaggcagaa ggaagcaggt gtgagggcag tgcaaggagg
     6601 gagcacaacc cccagctccc gctccgggct ccgacttgtg cacaggcaga gcccagaccc
     6661 tggaggaaat cctacctttg aattcaagaa catttgggga atttggaaat ctctttgccc
     6721 ccaaaccccc attctgtcct acctttaatc aggtcctgct cagcagtgag agcagatgag
     6781 gtgaaaaggc caagaggttt ggctcctgcc cactgatagc ccctctcccc gcagtgtttg
     6841 tgtgtcaagt ggcaaagctg ttcttcctgg tgaccctgat tatatccagt aacacataga
     6901 ctgtgcgcat aggcctgctt tgtctcctct atcctgggct tttgttttgc tttttagttt
     6961 tgcttttagt ttttctgtcc cttttattta acgcaccgac tagacacaca aagcagttga
     7021 atttttatat atatatctgt atattgcaca attataaact cattttgctt gtggctccac
     7081 acacacaaaa aaagacctgt taaaattata cctgttgctt aattacaata tttctgataa
     7141 ccatagcata ggacaaggga aaataaaaaa agaaaaaaaa gaaaaaaaaa cgacaaatct
     7201 gtctgctggt cacttcttct gtccaagcag attcgtggtc ttttcctcgc ttctttcaag
     7261 ggctttcctg tgccaggtga aggaggctcc aggcagcacc caggttttgc actcttgttt
     7321 ctcccgtgct tgtgaaagag gtcccaaggt tctgggtgca ggagcgctcc cttgacctgc
     7381 tgaagtccgg aacgtagtcg gcacagcctg gtcgccttcc acctctggga gctggagtcc
     7441 actggggtgg cctgactccc ccagtcccct tcccgtgacc tggtcagggt gagcccatgt
     7501 ggagtcagcc tcgcaggcct ccctgccagt agggtccgag tgtgtttcat ccttcccact
     7561 ctgtcgagcc tgggggctgg agcggagacg ggaggcctgg cctgtctcgg aacctgtgag
     7621 ctgcaccagg tagaacgcca gggaccccag aatcatgtgc gtcagtccaa ggggtcccct
     7681 ccaggagtag tgaagactcc agaaatgtcc ctttcttctc ccccatccta cgagtaattg
     7741 catttgcttt tgtaattctt aatgagcaat atctgctaga gagtttagct gtaacagttc
     7801 tttttgatca tcttttttta ataattagaa acaccaaaaa aatccagaaa cttgttcttc
     7861 caaagcagag agcattataa tcaccagggc caaaagcttc cctccctgct gtcattgctt
     7921 cttctgaggc ctgaatccaa aagaaaaaca gccataggcc ctttcagtgg ccgggctacc
     7981 cgtgagccct tcggaggacc agggctgggg cagcctctgg gcccacatcc ggggccagct
     8041 ccggcgtgtg ttcagtgtta gcagtgggtc atgatgctct ttcccaccca gcctgggata
     8101 ggggcagagg aggcgaggag gccgttgccg ctgatgtttg gccgtgaaca ggtgggtgtc
     8161 tgcgtgcgtc cacgtgcgtg ttttctgact gacatgaaat cgacgcccga gttagcctca
     8221 cccggtgacc tctagccctg cccggatgga gcggggccca cccggttcag tgtttctggg
     8281 gagctggaca gtggagtgca aaaggcttgc agaacttgaa gcctgctcct tcccttgcta
     8341 ccacggcctc ctttccgttt gatttgtcac tgcttcaatc aataacagcc gctccagagt
     8401 cagtagtcaa tgaatatatg accaaatatc accaggactg ttactcaatg tgtgccgagc
     8461 ccttgcccat gctgggctcc cgtgtatctg gacactgtaa cgtgtgctgt gtttgctccc
     8521 cttccccttc cttctttgcc ctttacttgt ctttctgggg tttttctgtt tgggtttggt
     8581 ttggttttta tttctccttt tgtgttccaa acatgaggtt ctctctactg gtcctcttaa
     8641 ctgtggtgtt gaggcttata tttgtgtaat ttttggtggg tgaaaggaat tttgctaagt
     8701 aaatctcttc tgtgtttgaa ctgaagtctg tattgtaact atgtttaaag taattgttcc
     8761 agagacaaat atttctagac actttttctt tacaaacaaa agcattcgga gggaggggga
     8821 tggtgactga gatgagaggg gagagctgaa cagatgaccc ctgcccagat cagccagaag
     8881 ccacccaaag cagtggagcc caggagtccc actccaagcc agcaagccga atagctgatg
     8941 tgttgccact ttccaagtca ctgcaaaacc aggttttgtt ccgcccagtg gattcttgtt
     9001 ttgcttcccc tccccccgag attattacca ccatcccgtg cttttaagga aaggcaagat
     9061 tgatgtttcc ttgaggggag ccaggagggg atgtgtgtgt gcagagctga agagctgggg
     9121 agaatggggc tgggcccacc caagcaggag gctgggacgc tctgctgtgg gcacaggtca
     9181 ggctaatgtt ggcagatgca gctcttcctg gacaggccag gtggtgggca ttctctctcc
     9241 aaggtgtgcc ccgtgggcat tactgtttaa gacacttccg tcacatccca ccccatcctc
     9301 cagggctcaa cactgtgaca tctctattcc ccaccctccc cttcccaggg caataaaatg
     9361 accatggagg gggcttgcac tctcttggct gtcacccgat cgccagcaaa acttagatgt
     9421 gagaaaaccc cttcccattc catggcgaaa acatctcctt agaaaagcca ttaccctcat
     9481 taggcatggt tttgggctcc caaaacacct gacagcccct ccctcctctg agaggcggag
     9541 agtgctgact gtagtgacca ttgcatgccg ggtgcagcat ctggaagagc taggcagggt
     9601 gtctgccccc tcctgagttg aagtcatgct cccctgtgcc agcccagagg ccgagagcta
     9661 tggacagcat tgccagtaac acaggccacc ctgtgcagaa gggagctggc tccagcctgg
     9721 aaacctgtct gaggttggga gaggtgcact tggggcacag ggagaggccg ggacacactt
     9781 agctggagat gtctctaaaa gccctgtatc gtattcacct tcagtttttg tgttttggga
     9841 caattacttt agaaaataag taggtcgttt taaaaacaaa aattattgat tgcttttttg
     9901 tagtgttcag aaaaaaggtt ctttgtgtat agccaaatga ctgaaagcac tgatatattt
     9961 aaaaacaaaa ggcaatttat taaggaaatt tgtaccattt cagtaaacct gtctgaatgt
    10021 acctgtatac gtttcaaaaa cacccccccc ccactgaatc cctgtaacct atttattata
    10081 taaagagttt gccttataaa tttacataaa aatgtccgtt tgtgtctttt gttgtaaaaa
    10141 tcaagtgatt ttttcataag gttcttttac tattggaaaa gatgggcagc acgcagtttt
    10201 attttatttt tgtaagtttt ttaatacatg tgaaagcaaa gaatactcag catgcctttc
    10261 taagtgacgc gtttgcacct tttgttggga agtactgtat cctgtgctgt tagcattctc
    10321 gataaatctc tctgtgaaag tga
//
