LOCUS       NM_004991               5462 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens MDS1 and EVI1 complex locus (MECOM), transcript
            variant 4, mRNA.
ACCESSION   NM_004991
VERSION     NM_004991.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Akiyama,H., Kantarjian,H., Jabbour,E., Issa,G., Haddad,F.G.,
            Short,N.J., Hu,S., Ishizawa,J., Andreeff,M. and Sasaki,K.
  TITLE     Outcome of 3q26.2/MECOM rearrangements in chronic myeloid leukemia
  JOURNAL   Int J Hematol 120 (2), 203-211 (2024)
   PUBMED   38748089
  REMARK    GeneRIF: Outcome of 3q26.2/MECOM rearrangements in chronic myeloid
            leukemia.
REFERENCE   2  (bases 1 to 5462)
  AUTHORS   Pastoors,D., Havermans,M., Mulet-Lazaro,R., Brian,D., Noort,W.,
            Grasel,J., Hoogenboezem,R., Smeenk,L., Demmers,J.A.A., Milsom,M.D.,
            Enver,T., Groen,R.W.J., Bindels,E. and Delwel,R.
  TITLE     Oncogene EVI1 drives acute myeloid leukemia via a targetable
            interaction with CTBP2
  JOURNAL   Sci Adv 10 (20), eadk9076 (2024)
   PUBMED   38748792
  REMARK    GeneRIF: Oncogene EVI1 drives acute myeloid leukemia via a
            targetable interaction with CTBP2.
REFERENCE   3  (bases 1 to 5462)
  AUTHORS   Ammeti,D., Marzollo,A., Gabelli,M., Zanchetta,M.E.,
            Tretti-Parenzan,C., Bottega,R., Capaci,V., Biffi,A., Savoia,A.,
            Bresolin,S. and Faleschini,M.
  TITLE     A novel mutation in MECOM affects MPL regulation in vitro and
            results in thrombocytopenia and bone marrow failure
  JOURNAL   Br J Haematol 203 (5), 852-859 (2023)
   PUBMED   37610030
  REMARK    GeneRIF: A novel mutation in MECOM affects MPL regulation in vitro
            and results in thrombocytopenia and bone marrow failure.
REFERENCE   4  (bases 1 to 5462)
  AUTHORS   Voit,R.A. and Sankaran,V.G.
  TITLE     MECOM Deficiency: from Bone Marrow Failure to Impaired B-Cell
            Development
  JOURNAL   J Clin Immunol 43 (6), 1052-1066 (2023)
   PUBMED   37407873
  REMARK    GeneRIF: MECOM Deficiency: from Bone Marrow Failure to Impaired
            B-Cell Development.
            Review article
REFERENCE   5  (bases 1 to 5462)
  AUTHORS   Haas,K., Kundi,M., Sperr,W.R., Esterbauer,H., Ludwig,W.D.,
            Ratei,R., Koller,E., Gruener,H., Sauerland,C., Fonatsch,C.,
            Valent,P. and Wieser,R.
  TITLE     Expression and prognostic significance of different mRNA 5'-end
            variants of the oncogene EVI1 in 266 patients with de novo AML:
            EVI1 and MDS1/EVI1 overexpression both predict short remission
            duration
  JOURNAL   Genes Chromosomes Cancer 47 (4), 288-298 (2008)
   PUBMED   18181178
  REMARK    GeneRIF: EVI1 and MDS1/EVI1 overexpression is associated with acute
            myeloid leukemia
REFERENCE   6  (bases 1 to 5462)
  AUTHORS   Aytekin,M., Vinatzer,U., Musteanu,M., Raynaud,S. and Wieser,R.
  TITLE     Regulation of the expression of the oncogene EVI1 through the use
            of alternative mRNA 5'-ends
  JOURNAL   Gene 356, 160-168 (2005)
   PUBMED   16014322
  REMARK    GeneRIF: The general expression patterns of the EVI1 5'-end
            variants in a panel of 20 human tissues were similar, while
            pronounced differences were noted in response to all-trans retinoic
            acid.
REFERENCE   7  (bases 1 to 5462)
  AUTHORS   Mochizuki,N., Shimizu,S., Nagasawa,T., Tanaka,H., Taniwaki,M.,
            Yokota,J. and Morishita,K.
  TITLE     A novel gene, MEL1, mapped to 1p36.3 is highly homologous to the
            MDS1/EVI1 gene and is transcriptionally activated in
            t(1;3)(p36;q21)-positive leukemia cells
  JOURNAL   Blood 96 (9), 3209-3214 (2000)
   PUBMED   11050005
REFERENCE   8  (bases 1 to 5462)
  AUTHORS   Nucifora,G., Begy,C.R., Kobayashi,H., Roulston,D., Claxton,D.,
            Pedersen-Bjergaard,J., Parganas,E., Ihle,J.N. and Rowley,J.D.
  TITLE     Consistent intergenic splicing and production of multiple
            transcripts between AML1 at 21q22 and unrelated genes at 3q26 in
            (3;21)(q26;q22) translocations
  JOURNAL   Proc Natl Acad Sci U S A 91 (9), 4004-4008 (1994)
   PUBMED   8171026
REFERENCE   9  (bases 1 to 5462)
  AUTHORS   Mitani,K., Ogawa,S., Tanaka,T., Miyoshi,H., Kurokawa,M., Mano,H.,
            Yazaki,Y., Ohki,M. and Hirai,H.
  TITLE     Generation of the AML1-EVI-1 fusion gene in the t(3;21)(q26;q22)
            causes blastic crisis in chronic myelocytic leukemia
  JOURNAL   EMBO J 13 (3), 504-510 (1994)
   PUBMED   8313895
REFERENCE   10 (bases 1 to 5462)
  AUTHORS   Morishita,K., Parganas,E., Douglass,E.C. and Ihle,J.N.
  TITLE     Unique expression of the human Evi-1 gene in an endometrial
            carcinoma cell line: sequence of cDNAs and structure of
            alternatively spliced transcripts
  JOURNAL   Oncogene 5 (7), 963-971 (1990)
   PUBMED   2115646
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC348381.1, AL700380.1,
            AK304098.1, CR541866.1, BC130520.1 and BX647613.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_004991.3.
            
            Summary: The protein encoded by this gene is a transcriptional
            regulator and oncoprotein that may be involved in hematopoiesis,
            apoptosis, development, and cell differentiation and proliferation.
            The encoded protein can interact with CTBP1, SMAD3, CREBBP, KAT2B,
            MAPK8, and MAPK9. This gene can undergo translocation with the AML1
            gene, resulting in overexpression of this gene and the onset of
            leukemia. Several transcript variants encoding a few different
            isoforms have been found for this gene. [provided by RefSeq, Mar
            2011].
            
            Transcript Variant: This variant (4, also known as MDS1/EVI1)
            differs in the 5' UTR and 5' coding region, and uses an alternate
            in-frame splice site in the central coding region, compared to
            variant 1. The encoded isoform (c) has a distinct N-terminus and is
            longer than isoform a. There are no publicly available full-length
            transcripts representing this variant, but it is supported by
            cloning evidence in PMID:11050005.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038195.2676794.1,
                                           SRR14038197.104651.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000651503.2/ ENSP00000498411.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-97                DC348381.1         64-160
            98-238              AL700380.1         99-239
            239-423             AK304098.1         248-432
            424-715             CR541866.1         84-375
            716-3175            AK304098.1         725-3184
            3176-4095           BC130520.1         2580-3499
            4096-5462           BX647613.1         4098-5464
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q26.2"
     gene            1..5462
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /note="MDS1 and EVI1 complex locus"
                     /db_xref="GeneID:2122"
                     /db_xref="HGNC:HGNC:3498"
                     /db_xref="MIM:165215"
     exon            1..377
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     CDS             341..4060
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /EC_number="2.1.1.367"
                     /note="isoform c is encoded by transcript variant 4; MDS1
                     and EVI1 complex locus protein EVI1; MDS1 and EVI1 complex
                     locus protein MDS1; oncogene EVI1; myelodysplasia
                     syndrome-associated protein 1; zinc finger protein Evi1;
                     AML1-EVI-1 fusion protein; PR domain 3; ecotropic virus
                     integration site 1 protein homolog; histone-lysine
                     N-methyltransferase MECOM"
                     /codon_start=1
                     /product="histone-lysine N-methyltransferase MECOM isoform
                     c"
                     /protein_id="NP_004982.2"
                     /db_xref="CCDS:CCDS93425.1"
                     /db_xref="GeneID:2122"
                     /db_xref="HGNC:HGNC:3498"
                     /db_xref="MIM:165215"
                     /translation="MRSKGRARKLATNNECVYGNYPEIPLEEMPDADGVASTPSLNIQ
                     EPCSPATSSEAFTPKEGSPYKAPIYIPDDIPIPAEFELRESNMPGAGLGIWTKRKIEV
                     GEKFGPYVGEQRSNLKDPSYGWEILDEFYNVKFCIDASQPDVGSWLKYIRFAGCYDQH
                     NLVACQINDQIFYRVVADIAPGEELLLFMKSEDYPHETMAPDIHEERQYRCEDCDQLF
                     ESKAELADHQKFPCSTPHSAFSMVEEDFQQKLESENDLQEIHTIQECKECDQVFPDLQ
                     SLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVFTDPSNL
                     QRHIRSQHVGARAHACPECGKTFATSSGLKQHKHIHSSVKPFICEVCHKSYTQFSNLC
                     RHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNHFAAGGFFGQGISLPGTPA
                     MDKTSMVNMSHANPGLADYFGANRHPAGLTFPTAPGFSFSFPGLFPSGLYHRPPLIPA
                     SSPVKGLSSTEQTNKSQSPLMTHPQILPATQDILKALSKHPSVGDNKPVELQPERSSE
                     ERPFEKISDQSESSDLDDVSTPSGSDLETTSGSDLESDIESDKEKFKENGKMFKDKVS
                     PLQNLASINNKKEYSNHSIFSPSLEEQTAVSGAVNDSIKAIASIAEKYFGSTGLVGLQ
                     DKKVGALPYPSMFPLPFFPAFSQSMYPFPDRDLRSLPLKMEPQSPGEVKKLQKGSSES
                     PFDLTTKRKDEKPLTPVPSKPPVTPATSQDQPLDLSMGSRSRASGTKLTEPRKNHVFG
                     GKKGSNVESRPASDGSLQHARPTPFFMDPIYRVEKRKLTDPLEALKEKYLRPSPGFLF
                     HPQFQLPDQRTWMSAIENMAEKLESFSALKPEASELLQSVPSMFNFRAPPNALPENLL
                     RKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIH
                     NKEKPFKCHLCDRCFGQQTNLDRHLKKHENGNMSGTATSSPHSELESTGAILDDKEDA
                     YFTEIRNFIGNSNHGSQSPRNVEERMNGSHFKDEKALVTSQNSDLLDDEEVEDEVLLD
                     EEDEDNDITGKTGKEPVTSNLHEGNPEDDYEETSALEMSCKTSPVRYKEEEYKSGLSA
                     LDHIRHFTDSLKMRKMEDNQYSEAELSSFSTSHVPEELKQPLHRKSKSQAYAMMLSLS
                     DKESLHSTSHSSSNVWHSMARAAAESSAIQSISHV"
     exon            378..715
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            716..850
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            851..953
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            954..1170
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1171..1318
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1319..1472
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1473..2829
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            2830..2917
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            2918..2944
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            2945..3111
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            3112..3189
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            3190..3359
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            3360..3504
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            3505..3741
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            3742..3925
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            3926..5462
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5442..5447
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /note="hexamer: AATAAA"
     polyA_site      5462
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /note="major polyA site"
ORIGIN      
        1 gctgagatgt tggaggggcg tctagcgcgc atgtgcgaag gtgtccaaac tgacaatgct
       61 ggagagatag cgagtgtgga ttgagagaaa gggagagagg gagggagaga gagtgaaaga
      121 agaaaataca gagagtgagt gtgtggaaga gagagagaaa caggagagaa acaggaggga
      181 gggagagaga gagagagaga gagagagaga gagagagaga gagagagaga gagagagaca
      241 ggagagagag ggagggagcg agagggagag caaaagaagg aaaggatcca agaaaaaaaa
      301 gccccaacca cacaccagcg gctgcaggac tgggcacagc atgagatcca aaggcagggc
      361 aaggaaactg gccacaaata atgagtgtgt atatggcaac taccctgaaa tacctttgga
      421 agaaatgcca gatgcagatg gagtagccag cactccctcc ctcaatattc aagagccatg
      481 ctctcctgcc acatccagtg aagcattcac tccaaaggag ggttctcctt acaaagcccc
      541 catctacatc cctgatgata tccccattcc tgctgagttt gaacttcgag agtcaaatat
      601 gcctggggca ggactaggaa tatggaccaa aaggaagatc gaagtaggtg aaaagtttgg
      661 gccttatgtg ggagagcaga ggtcaaacct gaaagacccc agttatggat gggagatctt
      721 agacgaattt tacaatgtga agttctgcat agatgccagt caaccagatg ttggaagctg
      781 gctcaagtac attagattcg ctggctgtta tgatcagcac aaccttgttg catgccagat
      841 aaatgatcag atattctata gagtagttgc agacattgcg ccgggagagg agcttctgct
      901 gttcatgaag agcgaagact atccccatga aactatggcg ccggatatcc acgaagaacg
      961 gcaatatcgc tgcgaagact gtgaccagct ctttgaatct aaggctgaac tagcagatca
     1021 ccaaaagttt ccatgcagta ctcctcactc agcattttca atggttgaag aggactttca
     1081 gcaaaaactc gaaagcgaga atgatctcca agagatacac acgatccagg agtgtaagga
     1141 atgtgaccaa gtttttcctg atttgcaaag cctggagaaa cacatgctgt cacatactga
     1201 agagagggaa tacaagtgtg atcagtgtcc caaggcattt aactggaagt ccaatttaat
     1261 tcgccaccag atgtcacatg acagtggaaa gcactatgaa tgtgaaaact gtgccaaggt
     1321 tttcacggac cctagcaacc ttcagcggca cattcgctct cagcatgtcg gtgcccgggc
     1381 ccatgcatgc ccggagtgtg gcaaaacgtt tgccacttcg tcgggcctca aacaacacaa
     1441 gcacatccac agcagtgtga agccctttat ctgtgaggtc tgccataaat cctatactca
     1501 gttttcaaac ctttgccgtc ataagcgcat gcatgctgat tgcagaaccc aaatcaagtg
     1561 caaagactgt ggacaaatgt tcagcactac gtcttcctta aataaacaca ggaggttttg
     1621 tgagggcaag aaccattttg cggcaggtgg attttttggc caaggcattt cacttcctgg
     1681 aaccccagct atggataaaa cgtccatggt taatatgagt catgccaacc cgggccttgc
     1741 tgactatttt ggcgccaata ggcatcctgc tggtcttacc tttccaacag ctcctggatt
     1801 ttcttttagc ttccctggtc tgtttccttc cggcttgtac cacaggcctc ctttgatacc
     1861 tgctagttct cctgttaaag gactatcaag tactgaacag acaaacaaaa gtcaaagtcc
     1921 cctcatgaca catcctcaga tactgccagc tacacaggat attttgaagg cactatctaa
     1981 acacccatct gtaggggaca ataagccagt ggagctccag cccgagaggt cctctgaaga
     2041 gaggcccttt gagaaaatca gtgaccagtc agagagtagt gaccttgatg atgtcagtac
     2101 accaagtggc agtgacctgg aaacaacctc gggctctgat ctggaaagtg acattgaaag
     2161 tgataaagag aaatttaaag aaaatggtaa aatgttcaaa gacaaagtaa gccctcttca
     2221 gaatctggct tcaataaata ataagaaaga atacagcaat cattccattt tctcaccatc
     2281 tttagaggag cagactgcgg tgtcaggagc tgtgaatgat tctataaagg ctattgcttc
     2341 tattgctgaa aaatactttg gttcaacagg actggtgggg ctgcaagaca aaaaagttgg
     2401 agctttacct tacccttcca tgtttcccct cccatttttt ccagcattct ctcaatcaat
     2461 gtacccattt cctgatagag acttgagatc gttacctttg aaaatggaac cccaatcacc
     2521 aggtgaagta aagaaactgc agaagggcag ctctgagtcc ccctttgatc tcaccactaa
     2581 gcgaaaggat gagaagccct tgactccagt cccctccaag cctccagtga cacctgccac
     2641 aagccaagac cagcccctgg atctaagtat gggcagtagg agtagagcca gtgggacaaa
     2701 gctgactgag cctcgaaaaa accacgtgtt tgggggaaaa aaaggaagca acgtcgaatc
     2761 aagacctgct tcagatggtt ccttgcagca tgcaagaccc actcctttct ttatggaccc
     2821 tatttacaga gtagagaaaa gaaaactaac tgacccactt gaagctttaa aagagaaata
     2881 cttgaggcct tctccaggat tcttgtttca cccacaattc caactgcctg atcagagaac
     2941 ttggatgtca gctattgaaa acatggcaga aaagctagag agcttcagtg ccctgaaacc
     3001 tgaggccagt gagctcttac agtcagtgcc ctctatgttc aacttcaggg cgcctcccaa
     3061 tgccctgcca gagaaccttc tgcggaaggg aaaggagcgc tatacctgca gatactgtgg
     3121 caagattttt ccaaggtctg caaacctaac acggcacttg agaacccaca caggagagca
     3181 gccttacaga tgcaaatact gtgacagatc atttagcata tcttctaact tgcaaaggca
     3241 tgttcgcaac atccacaata aagagaagcc atttaagtgt cacttatgtg ataggtgttt
     3301 tggtcaacaa accaatttag acagacacct aaagaaacat gagaatggga acatgtccgg
     3361 tacagcaaca tcgtcgcctc attctgaact ggaaagtaca ggtgcgattc tggatgacaa
     3421 agaagatgct tacttcacag aaattcgaaa tttcattggg aacagcaacc atggcagcca
     3481 atctcccagg aatgtggagg agagaatgaa tggcagtcat tttaaagatg aaaaggcttt
     3541 ggtgaccagt caaaattcag acttgctgga tgatgaagaa gttgaagatg aggtgttgtt
     3601 agatgaggag gatgaagaca atgatattac tggaaaaaca ggaaaggaac cagtgacaag
     3661 taatttacat gaaggaaacc ctgaggatga ctatgaagaa accagtgccc tggagatgag
     3721 ttgcaagaca tccccagtga ggtataaaga ggaagaatat aaaagtggac tttctgctct
     3781 agatcatata aggcacttca cagatagcct caaaatgagg aaaatggaag ataatcaata
     3841 ttctgaagct gagctgtctt cttttagtac ttcccatgtg ccagaggaac ttaagcagcc
     3901 gttacacaga aagtccaaat cgcaggcata tgctatgatg ctgtcactgt ctgacaagga
     3961 gtccctccat tctacatccc acagttcttc caacgtgtgg cacagtatgg ccagggctgc
     4021 ggcggaatcc agtgctatcc agtccataag ccacgtatga cgttatcaag gttgaccaga
     4081 gtgggaccaa gtccaacagt agcatggctc tttcatatag gactatttac aagactgctg
     4141 agcagaatgc cttataaacc tgcagggtca ctcatctaaa gtctagtgac cttaaactga
     4201 atgatttaaa aaagaaaaga aagaaaaaag aaactattta ttctcgatat tttgttttgc
     4261 acagcaaagg cagctgctga cttctggaag atcaatcaat gcgacttaaa gtgattcagt
     4321 gaaaacaaaa aacttggtgg gctgaaggca tcttccagtt taccccacct tagggtatgg
     4381 gtgggtgaga agggcagttg agatggcagc attgatatga atgaacactc catagaaact
     4441 gaattctctt ttgtacaaga tcacctgaca tgattgggaa cagttgcttt taattacaga
     4501 tttaattttt ttcttcgtta aagttttatg taatttaacc ctttgaagac agaagtagtt
     4561 ggatgaaatg cacagtcaat tattatagaa actgataaca gggagtactt gttccccctt
     4621 ttgccttctt aagtacattg tttaaaacta gggaaaaagg gtatgtgtat attgtaaact
     4681 atggatgtta acactcaaag aggttaagtc agtgaagtaa cctattcatc accagtaccg
     4741 ctgtaccact aataaattgt ttgccaaatc cttgtaataa catcttaatt ttagacaatc
     4801 atgtcactgt ttttaatgtt tatttttttg tgtgtgttgc gtgtatcatg tatttatttg
     4861 ttggcaaact attgtttgtt gattaaaata gcactgttcc agtcagccac tactttatga
     4921 cgtctgaggc acaccccttt ccgaatttca aggaccaagg tgacccgacc tgtgtatgag
     4981 agtgccaaat ggtgtttggc ttttcttaac attccttttt gtttgtttgt tttgttttcc
     5041 ttcttaatga actaaatacg aatagatgca acttagtttt tgtaatactg aaatcgattc
     5101 aattgtataa acgattataa tttctttcat ggaagcatga ttcttctgat taaaaactgt
     5161 actccatatt ttatgctggt tgtctgcaag cttgtgcgat gttatgttca tgttaatcct
     5221 atttgtaaaa tgaagtgttc ccaaccttat gttaaaagag agaagtaaat aacagactgt
     5281 attcagttat tttgcccttt attgaggaac cagatttgtt ttctttttgt ttgtaatctc
     5341 attttgaaat aatcagcaag ttgaggtact ttcttcaaat gctttgtaca atataaactg
     5401 ttatgccttt cagtgcatta ctatgggagg agcaactaaa aaataaagac ttacaaaaag
     5461 ga
//
