LOCUS       NM_001366466            5435 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens MDS1 and EVI1 complex locus (MECOM), transcript
            variant 8, mRNA.
ACCESSION   NM_001366466 XM_005247215
VERSION     NM_001366466.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Akiyama,H., Kantarjian,H., Jabbour,E., Issa,G., Haddad,F.G.,
            Short,N.J., Hu,S., Ishizawa,J., Andreeff,M. and Sasaki,K.
  TITLE     Outcome of 3q26.2/MECOM rearrangements in chronic myeloid leukemia
  JOURNAL   Int J Hematol 120 (2), 203-211 (2024)
   PUBMED   38748089
  REMARK    GeneRIF: Outcome of 3q26.2/MECOM rearrangements in chronic myeloid
            leukemia.
REFERENCE   2  (bases 1 to 5435)
  AUTHORS   Pastoors,D., Havermans,M., Mulet-Lazaro,R., Brian,D., Noort,W.,
            Grasel,J., Hoogenboezem,R., Smeenk,L., Demmers,J.A.A., Milsom,M.D.,
            Enver,T., Groen,R.W.J., Bindels,E. and Delwel,R.
  TITLE     Oncogene EVI1 drives acute myeloid leukemia via a targetable
            interaction with CTBP2
  JOURNAL   Sci Adv 10 (20), eadk9076 (2024)
   PUBMED   38748792
  REMARK    GeneRIF: Oncogene EVI1 drives acute myeloid leukemia via a
            targetable interaction with CTBP2.
REFERENCE   3  (bases 1 to 5435)
  AUTHORS   Ammeti,D., Marzollo,A., Gabelli,M., Zanchetta,M.E.,
            Tretti-Parenzan,C., Bottega,R., Capaci,V., Biffi,A., Savoia,A.,
            Bresolin,S. and Faleschini,M.
  TITLE     A novel mutation in MECOM affects MPL regulation in vitro and
            results in thrombocytopenia and bone marrow failure
  JOURNAL   Br J Haematol 203 (5), 852-859 (2023)
   PUBMED   37610030
  REMARK    GeneRIF: A novel mutation in MECOM affects MPL regulation in vitro
            and results in thrombocytopenia and bone marrow failure.
REFERENCE   4  (bases 1 to 5435)
  AUTHORS   Voit,R.A. and Sankaran,V.G.
  TITLE     MECOM Deficiency: from Bone Marrow Failure to Impaired B-Cell
            Development
  JOURNAL   J Clin Immunol 43 (6), 1052-1066 (2023)
   PUBMED   37407873
  REMARK    GeneRIF: MECOM Deficiency: from Bone Marrow Failure to Impaired
            B-Cell Development.
            Review article
REFERENCE   5  (bases 1 to 5435)
  AUTHORS   Haas,K., Kundi,M., Sperr,W.R., Esterbauer,H., Ludwig,W.D.,
            Ratei,R., Koller,E., Gruener,H., Sauerland,C., Fonatsch,C.,
            Valent,P. and Wieser,R.
  TITLE     Expression and prognostic significance of different mRNA 5'-end
            variants of the oncogene EVI1 in 266 patients with de novo AML:
            EVI1 and MDS1/EVI1 overexpression both predict short remission
            duration
  JOURNAL   Genes Chromosomes Cancer 47 (4), 288-298 (2008)
   PUBMED   18181178
  REMARK    GeneRIF: EVI1 and MDS1/EVI1 overexpression is associated with acute
            myeloid leukemia
REFERENCE   6  (bases 1 to 5435)
  AUTHORS   Aytekin,M., Vinatzer,U., Musteanu,M., Raynaud,S. and Wieser,R.
  TITLE     Regulation of the expression of the oncogene EVI1 through the use
            of alternative mRNA 5'-ends
  JOURNAL   Gene 356, 160-168 (2005)
   PUBMED   16014322
  REMARK    GeneRIF: The general expression patterns of the EVI1 5'-end
            variants in a panel of 20 human tissues were similar, while
            pronounced differences were noted in response to all-trans retinoic
            acid.
REFERENCE   7  (bases 1 to 5435)
  AUTHORS   Mochizuki,N., Shimizu,S., Nagasawa,T., Tanaka,H., Taniwaki,M.,
            Yokota,J. and Morishita,K.
  TITLE     A novel gene, MEL1, mapped to 1p36.3 is highly homologous to the
            MDS1/EVI1 gene and is transcriptionally activated in
            t(1;3)(p36;q21)-positive leukemia cells
  JOURNAL   Blood 96 (9), 3209-3214 (2000)
   PUBMED   11050005
REFERENCE   8  (bases 1 to 5435)
  AUTHORS   Nucifora,G., Begy,C.R., Kobayashi,H., Roulston,D., Claxton,D.,
            Pedersen-Bjergaard,J., Parganas,E., Ihle,J.N. and Rowley,J.D.
  TITLE     Consistent intergenic splicing and production of multiple
            transcripts between AML1 at 21q22 and unrelated genes at 3q26 in
            (3;21)(q26;q22) translocations
  JOURNAL   Proc Natl Acad Sci U S A 91 (9), 4004-4008 (1994)
   PUBMED   8171026
REFERENCE   9  (bases 1 to 5435)
  AUTHORS   Mitani,K., Ogawa,S., Tanaka,T., Miyoshi,H., Kurokawa,M., Mano,H.,
            Yazaki,Y., Ohki,M. and Hirai,H.
  TITLE     Generation of the AML1-EVI-1 fusion gene in the t(3;21)(q26;q22)
            causes blastic crisis in chronic myelocytic leukemia
  JOURNAL   EMBO J 13 (3), 504-510 (1994)
   PUBMED   8313895
REFERENCE   10 (bases 1 to 5435)
  AUTHORS   Morishita,K., Parganas,E., Douglass,E.C. and Ihle,J.N.
  TITLE     Unique expression of the human Evi-1 gene in an endometrial
            carcinoma cell line: sequence of cDNAs and structure of
            alternatively spliced transcripts
  JOURNAL   Oncogene 5 (7), 963-971 (1990)
   PUBMED   2115646
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL700380.1, DC417787.1,
            AK308404.1 and AC078985.14.
            
            On Jun 1, 2019 this sequence version replaced NM_001366466.1.
            
            Summary: The protein encoded by this gene is a transcriptional
            regulator and oncoprotein that may be involved in hematopoiesis,
            apoptosis, development, and cell differentiation and proliferation.
            The encoded protein can interact with CTBP1, SMAD3, CREBBP, KAT2B,
            MAPK8, and MAPK9. This gene can undergo translocation with the AML1
            gene, resulting in overexpression of this gene and the onset of
            leukemia. Several transcript variants encoding a few different
            isoforms have been found for this gene. [provided by RefSeq, Mar
            2011].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038195.2148990.1,
                                           ERR4352443.436243.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-238               AL700380.1         2-239
            239-490             AL700380.1         242-493
            491-899             DC417787.1         180-588
            900-1472            AK308404.1         608-1180
            1473-2829           AC078985.14        89080-90436         c
            2830-2917           AC078985.14        86484-86571         c
            2918-3084           AC078985.14        75757-75923         c
            3085-3162           AC078985.14        74582-74659         c
            3163-3332           AC078985.14        68773-68942         c
            3333-3477           AC078985.14        66655-66799         c
            3478-3714           AC078985.14        63697-63933         c
            3715-3898           AC078985.14        62697-62880         c
            3899-5435           AC078985.14        57204-58740         c
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q26.2"
     gene            1..5435
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /note="MDS1 and EVI1 complex locus"
                     /db_xref="GeneID:2122"
                     /db_xref="HGNC:HGNC:3498"
                     /db_xref="MIM:165215"
     exon            1..377
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     CDS             341..4033
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /EC_number="2.1.1.367"
                     /note="isoform f is encoded by transcript variant 8; MDS1
                     and EVI1 complex locus protein EVI1; MDS1 and EVI1 complex
                     locus protein MDS1; oncogene EVI1; myelodysplasia
                     syndrome-associated protein 1; zinc finger protein Evi1;
                     AML1-EVI-1 fusion protein; PR domain 3; ecotropic virus
                     integration site 1 protein homolog; histone-lysine
                     N-methyltransferase MECOM"
                     /codon_start=1
                     /product="histone-lysine N-methyltransferase MECOM isoform
                     f"
                     /protein_id="NP_001353395.1"
                     /db_xref="CCDS:CCDS93424.1"
                     /db_xref="GeneID:2122"
                     /db_xref="HGNC:HGNC:3498"
                     /db_xref="MIM:165215"
                     /translation="MRSKGRARKLATNNECVYGNYPEIPLEEMPDADGVASTPSLNIQ
                     EPCSPATSSEAFTPKEGSPYKAPIYIPDDIPIPAEFELRESNMPGAGLGIWTKRKIEV
                     GEKFGPYVGEQRSNLKDPSYGWEILDEFYNVKFCIDASQPDVGSWLKYIRFAGCYDQH
                     NLVACQINDQIFYRVVADIAPGEELLLFMKSEDYPHETMAPDIHEERQYRCEDCDQLF
                     ESKAELADHQKFPCSTPHSAFSMVEEDFQQKLESENDLQEIHTIQECKECDQVFPDLQ
                     SLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVFTDPSNL
                     QRHIRSQHVGARAHACPECGKTFATSSGLKQHKHIHSSVKPFICEVCHKSYTQFSNLC
                     RHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNHFAAGGFFGQGISLPGTPA
                     MDKTSMVNMSHANPGLADYFGANRHPAGLTFPTAPGFSFSFPGLFPSGLYHRPPLIPA
                     SSPVKGLSSTEQTNKSQSPLMTHPQILPATQDILKALSKHPSVGDNKPVELQPERSSE
                     ERPFEKISDQSESSDLDDVSTPSGSDLETTSGSDLESDIESDKEKFKENGKMFKDKVS
                     PLQNLASINNKKEYSNHSIFSPSLEEQTAVSGAVNDSIKAIASIAEKYFGSTGLVGLQ
                     DKKVGALPYPSMFPLPFFPAFSQSMYPFPDRDLRSLPLKMEPQSPGEVKKLQKGSSES
                     PFDLTTKRKDEKPLTPVPSKPPVTPATSQDQPLDLSMGSRSRASGTKLTEPRKNHVFG
                     GKKGSNVESRPASDGSLQHARPTPFFMDPIYRVEKRKLTDPLEALKEKYLRPSPGFLF
                     HPQMSAIENMAEKLESFSALKPEASELLQSVPSMFNFRAPPNALPENLLRKGKERYTC
                     RYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCH
                     LCDRCFGQQTNLDRHLKKHENGNMSGTATSSPHSELESTGAILDDKEDAYFTEIRNFI
                     GNSNHGSQSPRNVEERMNGSHFKDEKALVTSQNSDLLDDEEVEDEVLLDEEDEDNDIT
                     GKTGKEPVTSNLHEGNPEDDYEETSALEMSCKTSPVRYKEEEYKSGLSALDHIRHFTD
                     SLKMRKMEDNQYSEAELSSFSTSHVPEELKQPLHRKSKSQAYAMMLSLSDKESLHSTS
                     HSSSNVWHSMARAAAESSAIQSISHV"
     exon            378..715
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            716..850
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            851..953
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            954..1170
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1171..1318
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1319..1472
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1473..2829
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            2830..2917
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            2918..3084
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            3085..3162
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            3163..3332
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            3333..3477
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            3478..3714
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            3715..3898
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            3899..5435
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5415..5420
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /note="hexamer: AATAAA"
     polyA_site      5435
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /note="major polyA site"
ORIGIN      
        1 gctgagatgt tggaggggcg tctagcgcgc atgtgcgaag gtgtccaaac tgacaatgct
       61 ggagagatag cgagtgtgga ttgagagaaa gggagagagg gagggagaga gagtgaaaga
      121 agaaaataca gagagtgagt gtgtggaaga gagagagaaa caggagagaa acaggaggga
      181 gggagagaga gagagagaga gagagagaga gagagagaga gagagagaga gagagagaca
      241 ggagagagag ggagggagcg agagggagag caaaagaagg aaaggatcca agaaaaaaaa
      301 gccccaacca cacaccagcg gctgcaggac tgggcacagc atgagatcca aaggcagggc
      361 aaggaaactg gccacaaata atgagtgtgt atatggcaac taccctgaaa tacctttgga
      421 agaaatgcca gatgcagatg gagtagccag cactccctcc ctcaatattc aagagccatg
      481 ctctcctgcc acatccagtg aagcattcac tccaaaggag ggttctcctt acaaagcccc
      541 catctacatc cctgatgata tccccattcc tgctgagttt gaacttcgag agtcaaatat
      601 gcctggggca ggactaggaa tatggaccaa aaggaagatc gaagtaggtg aaaagtttgg
      661 gccttatgtg ggagagcaga ggtcaaacct gaaagacccc agttatggat gggagatctt
      721 agacgaattt tacaatgtga agttctgcat agatgccagt caaccagatg ttggaagctg
      781 gctcaagtac attagattcg ctggctgtta tgatcagcac aaccttgttg catgccagat
      841 aaatgatcag atattctata gagtagttgc agacattgcg ccgggagagg agcttctgct
      901 gttcatgaag agcgaagact atccccatga aactatggcg ccggatatcc acgaagaacg
      961 gcaatatcgc tgcgaagact gtgaccagct ctttgaatct aaggctgaac tagcagatca
     1021 ccaaaagttt ccatgcagta ctcctcactc agcattttca atggttgaag aggactttca
     1081 gcaaaaactc gaaagcgaga atgatctcca agagatacac acgatccagg agtgtaagga
     1141 atgtgaccaa gtttttcctg atttgcaaag cctggagaaa cacatgctgt cacatactga
     1201 agagagggaa tacaagtgtg atcagtgtcc caaggcattt aactggaagt ccaatttaat
     1261 tcgccaccag atgtcacatg acagtggaaa gcactatgaa tgtgaaaact gtgccaaggt
     1321 tttcacggac cctagcaacc ttcagcggca cattcgctct cagcatgtcg gtgcccgggc
     1381 ccatgcatgc ccggagtgtg gcaaaacgtt tgccacttcg tcgggcctca aacaacacaa
     1441 gcacatccac agcagtgtga agccctttat ctgtgaggtc tgccataaat cctatactca
     1501 gttttcaaac ctttgccgtc ataagcgcat gcatgctgat tgcagaaccc aaatcaagtg
     1561 caaagactgt ggacaaatgt tcagcactac gtcttcctta aataaacaca ggaggttttg
     1621 tgagggcaag aaccattttg cggcaggtgg attttttggc caaggcattt cacttcctgg
     1681 aaccccagct atggataaaa cgtccatggt taatatgagt catgccaacc cgggccttgc
     1741 tgactatttt ggcgccaata ggcatcctgc tggtcttacc tttccaacag ctcctggatt
     1801 ttcttttagc ttccctggtc tgtttccttc cggcttgtac cacaggcctc ctttgatacc
     1861 tgctagttct cctgttaaag gactatcaag tactgaacag acaaacaaaa gtcaaagtcc
     1921 cctcatgaca catcctcaga tactgccagc tacacaggat attttgaagg cactatctaa
     1981 acacccatct gtaggggaca ataagccagt ggagctccag cccgagaggt cctctgaaga
     2041 gaggcccttt gagaaaatca gtgaccagtc agagagtagt gaccttgatg atgtcagtac
     2101 accaagtggc agtgacctgg aaacaacctc gggctctgat ctggaaagtg acattgaaag
     2161 tgataaagag aaatttaaag aaaatggtaa aatgttcaaa gacaaagtaa gccctcttca
     2221 gaatctggct tcaataaata ataagaaaga atacagcaat cattccattt tctcaccatc
     2281 tttagaggag cagactgcgg tgtcaggagc tgtgaatgat tctataaagg ctattgcttc
     2341 tattgctgaa aaatactttg gttcaacagg actggtgggg ctgcaagaca aaaaagttgg
     2401 agctttacct tacccttcca tgtttcccct cccatttttt ccagcattct ctcaatcaat
     2461 gtacccattt cctgatagag acttgagatc gttacctttg aaaatggaac cccaatcacc
     2521 aggtgaagta aagaaactgc agaagggcag ctctgagtcc ccctttgatc tcaccactaa
     2581 gcgaaaggat gagaagccct tgactccagt cccctccaag cctccagtga cacctgccac
     2641 aagccaagac cagcccctgg atctaagtat gggcagtagg agtagagcca gtgggacaaa
     2701 gctgactgag cctcgaaaaa accacgtgtt tgggggaaaa aaaggaagca acgtcgaatc
     2761 aagacctgct tcagatggtt ccttgcagca tgcaagaccc actcctttct ttatggaccc
     2821 tatttacaga gtagagaaaa gaaaactaac tgacccactt gaagctttaa aagagaaata
     2881 cttgaggcct tctccaggat tcttgtttca cccacaaatg tcagctattg aaaacatggc
     2941 agaaaagcta gagagcttca gtgccctgaa acctgaggcc agtgagctct tacagtcagt
     3001 gccctctatg ttcaacttca gggcgcctcc caatgccctg ccagagaacc ttctgcggaa
     3061 gggaaaggag cgctatacct gcagatactg tggcaagatt tttccaaggt ctgcaaacct
     3121 aacacggcac ttgagaaccc acacaggaga gcagccttac agatgcaaat actgtgacag
     3181 atcatttagc atatcttcta acttgcaaag gcatgttcgc aacatccaca ataaagagaa
     3241 gccatttaag tgtcacttat gtgataggtg ttttggtcaa caaaccaatt tagacagaca
     3301 cctaaagaaa catgagaatg ggaacatgtc cggtacagca acatcgtcgc ctcattctga
     3361 actggaaagt acaggtgcga ttctggatga caaagaagat gcttacttca cagaaattcg
     3421 aaatttcatt gggaacagca accatggcag ccaatctccc aggaatgtgg aggagagaat
     3481 gaatggcagt cattttaaag atgaaaaggc tttggtgacc agtcaaaatt cagacttgct
     3541 ggatgatgaa gaagttgaag atgaggtgtt gttagatgag gaggatgaag acaatgatat
     3601 tactggaaaa acaggaaagg aaccagtgac aagtaattta catgaaggaa accctgagga
     3661 tgactatgaa gaaaccagtg ccctggagat gagttgcaag acatccccag tgaggtataa
     3721 agaggaagaa tataaaagtg gactttctgc tctagatcat ataaggcact tcacagatag
     3781 cctcaaaatg aggaaaatgg aagataatca atattctgaa gctgagctgt cttcttttag
     3841 tacttcccat gtgccagagg aacttaagca gccgttacac agaaagtcca aatcgcaggc
     3901 atatgctatg atgctgtcac tgtctgacaa ggagtccctc cattctacat cccacagttc
     3961 ttccaacgtg tggcacagta tggccagggc tgcggcggaa tccagtgcta tccagtccat
     4021 aagccacgta tgacgttatc aaggttgacc agagtgggac caagtccaac agtagcatgg
     4081 ctctttcata taggactatt tacaagactg ctgagcagaa tgccttataa acctgcaggg
     4141 tcactcatct aaagtctagt gaccttaaac tgaatgattt aaaaaagaaa agaaagaaaa
     4201 aagaaactat ttattctcga tattttgttt tgcacagcaa aggcagctgc tgacttctgg
     4261 aagatcaatc aatgcgactt aaagtgattc agtgaaaaca aaaaacttgg tgggctgaag
     4321 gcatcttcca gtttacccca ccttagggta tgggtgggtg agaagggcag ttgagatggc
     4381 agcattgata tgaatgaaca ctccatagaa actgaattct cttttgtaca agatcacctg
     4441 acatgattgg gaacagttgc ttttaattac agatttaatt tttttcttcg ttaaagtttt
     4501 atgtaattta accctttgaa gacagaagta gttggatgaa atgcacagtc aattattata
     4561 gaaactgata acagggagta cttgttcccc cttttgcctt cttaagtaca ttgtttaaaa
     4621 ctagggaaaa agggtatgtg tatattgtaa actatggatg ttaacactca aagaggttaa
     4681 gtcagtgaag taacctattc atcaccagta ccgctgtacc actaataaat tgtttgccaa
     4741 atccttgtaa taacatctta attttagaca atcatgtcac tgtttttaat gtttattttt
     4801 ttgtgtgtgt tgcgtgtatc atgtatttat ttgttggcaa actattgttt gttgattaaa
     4861 atagcactgt tccagtcagc cactacttta tgacgtctga ggcacacccc tttccgaatt
     4921 tcaaggacca aggtgacccg acctgtgtat gagagtgcca aatggtgttt ggcttttctt
     4981 aacattcctt tttgtttgtt tgttttgttt tccttcttaa tgaactaaat acgaatagat
     5041 gcaacttagt ttttgtaata ctgaaatcga ttcaattgta taaacgatta taatttcttt
     5101 catggaagca tgattcttct gattaaaaac tgtactccat attttatgct ggttgtctgc
     5161 aagcttgtgc gatgttatgt tcatgttaat cctatttgta aaatgaagtg ttcccaacct
     5221 tatgttaaaa gagagaagta aataacagac tgtattcagt tattttgccc tttattgagg
     5281 aaccagattt gttttctttt tgtttgtaat ctcattttga aataatcagc aagttgaggt
     5341 actttcttca aatgctttgt acaatataaa ctgttatgcc tttcagtgca ttactatggg
     5401 aggagcaact aaaaaataaa gacttacaaa aagga
//
