LOCUS       NM_001163999            4803 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens MDS1 and EVI1 complex locus (MECOM), transcript
            variant 5, mRNA.
ACCESSION   NM_001163999
VERSION     NM_001163999.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Akiyama,H., Kantarjian,H., Jabbour,E., Issa,G., Haddad,F.G.,
            Short,N.J., Hu,S., Ishizawa,J., Andreeff,M. and Sasaki,K.
  TITLE     Outcome of 3q26.2/MECOM rearrangements in chronic myeloid leukemia
  JOURNAL   Int J Hematol 120 (2), 203-211 (2024)
   PUBMED   38748089
  REMARK    GeneRIF: Outcome of 3q26.2/MECOM rearrangements in chronic myeloid
            leukemia.
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Pastoors,D., Havermans,M., Mulet-Lazaro,R., Brian,D., Noort,W.,
            Grasel,J., Hoogenboezem,R., Smeenk,L., Demmers,J.A.A., Milsom,M.D.,
            Enver,T., Groen,R.W.J., Bindels,E. and Delwel,R.
  TITLE     Oncogene EVI1 drives acute myeloid leukemia via a targetable
            interaction with CTBP2
  JOURNAL   Sci Adv 10 (20), eadk9076 (2024)
   PUBMED   38748792
  REMARK    GeneRIF: Oncogene EVI1 drives acute myeloid leukemia via a
            targetable interaction with CTBP2.
REFERENCE   3  (bases 1 to 4803)
  AUTHORS   Ammeti,D., Marzollo,A., Gabelli,M., Zanchetta,M.E.,
            Tretti-Parenzan,C., Bottega,R., Capaci,V., Biffi,A., Savoia,A.,
            Bresolin,S. and Faleschini,M.
  TITLE     A novel mutation in MECOM affects MPL regulation in vitro and
            results in thrombocytopenia and bone marrow failure
  JOURNAL   Br J Haematol 203 (5), 852-859 (2023)
   PUBMED   37610030
  REMARK    GeneRIF: A novel mutation in MECOM affects MPL regulation in vitro
            and results in thrombocytopenia and bone marrow failure.
REFERENCE   4  (bases 1 to 4803)
  AUTHORS   Voit,R.A. and Sankaran,V.G.
  TITLE     MECOM Deficiency: from Bone Marrow Failure to Impaired B-Cell
            Development
  JOURNAL   J Clin Immunol 43 (6), 1052-1066 (2023)
   PUBMED   37407873
  REMARK    GeneRIF: MECOM Deficiency: from Bone Marrow Failure to Impaired
            B-Cell Development.
            Review article
REFERENCE   5  (bases 1 to 4803)
  AUTHORS   Haas,K., Kundi,M., Sperr,W.R., Esterbauer,H., Ludwig,W.D.,
            Ratei,R., Koller,E., Gruener,H., Sauerland,C., Fonatsch,C.,
            Valent,P. and Wieser,R.
  TITLE     Expression and prognostic significance of different mRNA 5'-end
            variants of the oncogene EVI1 in 266 patients with de novo AML:
            EVI1 and MDS1/EVI1 overexpression both predict short remission
            duration
  JOURNAL   Genes Chromosomes Cancer 47 (4), 288-298 (2008)
   PUBMED   18181178
  REMARK    GeneRIF: EVI1 and MDS1/EVI1 overexpression is associated with acute
            myeloid leukemia
REFERENCE   6  (bases 1 to 4803)
  AUTHORS   Aytekin,M., Vinatzer,U., Musteanu,M., Raynaud,S. and Wieser,R.
  TITLE     Regulation of the expression of the oncogene EVI1 through the use
            of alternative mRNA 5'-ends
  JOURNAL   Gene 356, 160-168 (2005)
   PUBMED   16014322
  REMARK    GeneRIF: The general expression patterns of the EVI1 5'-end
            variants in a panel of 20 human tissues were similar, while
            pronounced differences were noted in response to all-trans retinoic
            acid.
REFERENCE   7  (bases 1 to 4803)
  AUTHORS   Mochizuki,N., Shimizu,S., Nagasawa,T., Tanaka,H., Taniwaki,M.,
            Yokota,J. and Morishita,K.
  TITLE     A novel gene, MEL1, mapped to 1p36.3 is highly homologous to the
            MDS1/EVI1 gene and is transcriptionally activated in
            t(1;3)(p36;q21)-positive leukemia cells
  JOURNAL   Blood 96 (9), 3209-3214 (2000)
   PUBMED   11050005
REFERENCE   8  (bases 1 to 4803)
  AUTHORS   Nucifora,G., Begy,C.R., Kobayashi,H., Roulston,D., Claxton,D.,
            Pedersen-Bjergaard,J., Parganas,E., Ihle,J.N. and Rowley,J.D.
  TITLE     Consistent intergenic splicing and production of multiple
            transcripts between AML1 at 21q22 and unrelated genes at 3q26 in
            (3;21)(q26;q22) translocations
  JOURNAL   Proc Natl Acad Sci U S A 91 (9), 4004-4008 (1994)
   PUBMED   8171026
REFERENCE   9  (bases 1 to 4803)
  AUTHORS   Mitani,K., Ogawa,S., Tanaka,T., Miyoshi,H., Kurokawa,M., Mano,H.,
            Yazaki,Y., Ohki,M. and Hirai,H.
  TITLE     Generation of the AML1-EVI-1 fusion gene in the t(3;21)(q26;q22)
            causes blastic crisis in chronic myelocytic leukemia
  JOURNAL   EMBO J 13 (3), 504-510 (1994)
   PUBMED   8313895
REFERENCE   10 (bases 1 to 4803)
  AUTHORS   Morishita,K., Parganas,E., Douglass,E.C. and Ihle,J.N.
  TITLE     Unique expression of the human Evi-1 gene in an endometrial
            carcinoma cell line: sequence of cDNAs and structure of
            alternatively spliced transcripts
  JOURNAL   Oncogene 5 (7), 963-971 (1990)
   PUBMED   2115646
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC078985.14, BC143952.1 and
            BX647613.1.
            
            On Aug 14, 2020 this sequence version replaced NM_001163999.1.
            
            Summary: The protein encoded by this gene is a transcriptional
            regulator and oncoprotein that may be involved in hematopoiesis,
            apoptosis, development, and cell differentiation and proliferation.
            The encoded protein can interact with CTBP1, SMAD3, CREBBP, KAT2B,
            MAPK8, and MAPK9. This gene can undergo translocation with the AML1
            gene, resulting in overexpression of this gene and the onset of
            leukemia. Several transcript variants encoding a few different
            isoforms have been found for this gene. [provided by RefSeq, Mar
            2011].
            
            Transcript Variant: This variant (5) lacks a portion of the 5' UTR
            and 5' coding region, initiates translation at a downstream start
            codon, and lacks an alternate in-frame exon in the central coding
            region, compared to variant 1. The encoded isoform (d) is shorter
            than isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC143952.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                AC078985.14        119977-120002       c
            27-3436             BC143952.1         1-3410
            3437-4803           BX647613.1         4098-5464
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q26.2"
     gene            1..4803
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /note="MDS1 and EVI1 complex locus"
                     /db_xref="GeneID:2122"
                     /db_xref="HGNC:HGNC:3498"
                     /db_xref="MIM:165215"
     exon            1..80
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            81..215
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            216..318
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     CDS             270..3401
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /EC_number="2.1.1.367"
                     /note="isoform d is encoded by transcript variant 5; MDS1
                     and EVI1 complex locus protein EVI1; MDS1 and EVI1 complex
                     locus protein MDS1; oncogene EVI1; myelodysplasia
                     syndrome-associated protein 1; zinc finger protein Evi1;
                     AML1-EVI-1 fusion protein; PR domain 3; ecotropic virus
                     integration site 1 protein homolog; histone-lysine
                     N-methyltransferase MECOM"
                     /codon_start=1
                     /product="histone-lysine N-methyltransferase MECOM isoform
                     d"
                     /protein_id="NP_001157471.1"
                     /db_xref="GeneID:2122"
                     /db_xref="HGNC:HGNC:3498"
                     /db_xref="MIM:165215"
                     /translation="MKSEDYPHETMAPDIHEERQYRCEDCDQLFESKAELADHQKFPC
                     STPHSAFSMVEEDFQQKLESENDLQEIHTIQECKECDQVFPDLQSLEKHMLSHTEERE
                     YKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKQVFTDPSNLQRHIRSQHVGARA
                     HACPECGKTFATSSGLKQHKHIHSSVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQI
                     KCKDCGQMFSTTSSLNKHRRFCEGKNHFAAGGFFGQGISLPGTPAMDKTSMVNMSHAN
                     PGLADYFGANRHPAGLTFPTAPGFSFSFPGLFPSGLYHRPPLIPASSPVKGLSSTEQT
                     NKSQSPLMTHPQILPATQDILKALSKHPSVGDNKPVELQPERSSEERPFEKISDQSES
                     SDLDDVSTPSGSDLETTSGSDLESDIESDKEKFKENGKMFKDKVSPLQNLASINNKKE
                     YSNHSIFSPSLEEQTAVSGAVNDSIKAIASIAEKYFGSTGLVGLQDKKVGALPYPSMF
                     PLPFFPAFSQSMYPFPDRDLRSLPLKMEPQSPGEVKKLQKGSSESPFDLTTKRKDEKP
                     LTPVPSKPPVTPATSQDQPLDLSMGSRSRASGTKLTEPRKNHVFGGKKGSNVESRPAS
                     DGSLQHARPTPFFMDPIYRVEKRKLTDPLEALKEKYLRPSPGFLFHPQMSAIENMAEK
                     LESFSALKPEASELLQSVPSMFNFRAPPNALPENLLRKGKERYTCRYCGKIFPRSANL
                     TRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLD
                     RHLKKHENGNMSGTATSSPHSELESTGAILDDKEDAYFTEIRNFIGNSNHGSQSPRNV
                     EERMNGSHFKDEKALVTSQNSDLLDDEEVEDEVLLDEEDEDNDITGKTGKEPVTSNLH
                     EGNPEDDYEETSALEMSCKTSPVRYKEEEYKSGLSALDHIRHFTDSLKMRKMEDNQYS
                     EAELSSFSTSHVPEELKQPLHRKSKSQAYAMMLSLSDKESLHSTSHSSSNVWHSMARA
                     AAESSAIQSISHV"
     exon            319..535
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            536..683
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            684..840
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            841..2197
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            2198..2285
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            2286..2452
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            2453..2530
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            2531..2700
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            2701..2845
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            2846..3082
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            3083..3266
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     exon            3267..4803
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4783..4788
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /note="hexamer: AATAAA"
     polyA_site      4803
                     /gene="MECOM"
                     /gene_synonym="AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1;
                     PRDM3; RUSAT2"
                     /note="major polyA site"
ORIGIN      
        1 ccttgccaag taacagcttt gctgtccaac atcgtgtgct gcttcgcgag aaagtcacat
       61 tcggaccctt tggctagatt atcttagacg aattttacaa tgtgaagttc tgcatagatg
      121 ccagtcaacc agatgttgga agctggctca agtacattag attcgctggc tgttatgatc
      181 agcacaacct tgttgcatgc cagataaatg atcagatatt ctatagagta gttgcagaca
      241 ttgcgccggg agaggagctt ctgctgttca tgaagagcga agactatccc catgaaacta
      301 tggcgccgga tatccacgaa gaacggcaat atcgctgcga agactgtgac cagctctttg
      361 aatctaaggc tgaactagca gatcaccaaa agtttccatg cagtactcct cactcagcat
      421 tttcaatggt tgaagaggac tttcagcaaa aactcgaaag cgagaatgat ctccaagaga
      481 tacacacgat ccaggagtgt aaggaatgtg accaagtttt tcctgatttg caaagcctgg
      541 agaaacacat gctgtcacat actgaagaga gggaatacaa gtgtgatcag tgtcccaagg
      601 catttaactg gaagtccaat ttaattcgcc accagatgtc acatgacagt ggaaagcact
      661 atgaatgtga aaactgtgcc aagcaggttt tcacggaccc tagcaacctt cagcggcaca
      721 ttcgctctca gcatgtcggt gcccgggccc atgcatgccc ggagtgtggc aaaacgtttg
      781 ccacttcgtc gggcctcaaa caacacaagc acatccacag cagtgtgaag ccctttatct
      841 gtgaggtctg ccataaatcc tatactcagt tttcaaacct ttgccgtcat aagcgcatgc
      901 atgctgattg cagaacccaa atcaagtgca aagactgtgg acaaatgttc agcactacgt
      961 cttccttaaa taaacacagg aggttttgtg agggcaagaa ccattttgcg gcaggtggat
     1021 tttttggcca aggcatttca cttcctggaa ccccagctat ggataaaacg tccatggtta
     1081 atatgagtca tgccaacccg ggccttgctg actattttgg cgccaatagg catcctgctg
     1141 gtcttacctt tccaacagct cctggatttt cttttagctt ccctggtctg tttccttccg
     1201 gcttgtacca caggcctcct ttgatacctg ctagttctcc tgttaaagga ctatcaagta
     1261 ctgaacagac aaacaaaagt caaagtcccc tcatgacaca tcctcagata ctgccagcta
     1321 cacaggatat tttgaaggca ctatctaaac acccatctgt aggggacaat aagccagtgg
     1381 agctccagcc cgagaggtcc tctgaagaga ggccctttga gaaaatcagt gaccagtcag
     1441 agagtagtga ccttgatgat gtcagtacac caagtggcag tgacctggaa acaacctcgg
     1501 gctctgatct ggaaagtgac attgaaagtg ataaagagaa atttaaagaa aatggtaaaa
     1561 tgttcaaaga caaagtaagc cctcttcaga atctggcttc aataaataat aagaaagaat
     1621 acagcaatca ttccattttc tcaccatctt tagaggagca gactgcggtg tcaggagctg
     1681 tgaatgattc tataaaggct attgcttcta ttgctgaaaa atactttggt tcaacaggac
     1741 tggtggggct gcaagacaaa aaagttggag ctttacctta cccttccatg tttcccctcc
     1801 cattttttcc agcattctct caatcaatgt acccatttcc tgatagagac ttgagatcgt
     1861 tacctttgaa aatggaaccc caatcaccag gtgaagtaaa gaaactgcag aagggcagct
     1921 ctgagtcccc ctttgatctc accactaagc gaaaggatga gaagcccttg actccagtcc
     1981 cctccaagcc tccagtgaca cctgccacaa gccaagacca gcccctggat ctaagtatgg
     2041 gcagtaggag tagagccagt gggacaaagc tgactgagcc tcgaaaaaac cacgtgtttg
     2101 ggggaaaaaa aggaagcaac gtcgaatcaa gacctgcttc agatggttcc ttgcagcatg
     2161 caagacccac tcctttcttt atggacccta tttacagagt agagaaaaga aaactaactg
     2221 acccacttga agctttaaaa gagaaatact tgaggccttc tccaggattc ttgtttcacc
     2281 cacaaatgtc agctattgaa aacatggcag aaaagctaga gagcttcagt gccctgaaac
     2341 ctgaggccag tgagctctta cagtcagtgc cctctatgtt caacttcagg gcgcctccca
     2401 atgccctgcc agagaacctt ctgcggaagg gaaaggagcg ctatacctgc agatactgtg
     2461 gcaagatttt tccaaggtct gcaaacctaa cacggcactt gagaacccac acaggagagc
     2521 agccttacag atgcaaatac tgtgacagat catttagcat atcttctaac ttgcaaaggc
     2581 atgttcgcaa catccacaat aaagagaagc catttaagtg tcacttatgt gataggtgtt
     2641 ttggtcaaca aaccaattta gacagacacc taaagaaaca tgagaatggg aacatgtccg
     2701 gtacagcaac atcgtcgcct cattctgaac tggaaagtac aggtgcgatt ctggatgaca
     2761 aagaagatgc ttacttcaca gaaattcgaa atttcattgg gaacagcaac catggcagcc
     2821 aatctcccag gaatgtggag gagagaatga atggcagtca ttttaaagat gaaaaggctt
     2881 tggtgaccag tcaaaattca gacttgctgg atgatgaaga agttgaagat gaggtgttgt
     2941 tagatgagga ggatgaagac aatgatatta ctggaaaaac aggaaaggaa ccagtgacaa
     3001 gtaatttaca tgaaggaaac cctgaggatg actatgaaga aaccagtgcc ctggagatga
     3061 gttgcaagac atccccagtg aggtataaag aggaagaata taaaagtgga ctttctgctc
     3121 tagatcatat aaggcacttc acagatagcc tcaaaatgag gaaaatggaa gataatcaat
     3181 attctgaagc tgagctgtct tcttttagta cttcccatgt gccagaggaa cttaagcagc
     3241 cgttacacag aaagtccaaa tcgcaggcat atgctatgat gctgtcactg tctgacaagg
     3301 agtccctcca ttctacatcc cacagttctt ccaacgtgtg gcacagtatg gccagggctg
     3361 cggcggaatc cagtgctatc cagtccataa gccacgtatg acgttatcaa ggttgaccag
     3421 agtgggacca agtccaacag tagcatggct ctttcatata ggactattta caagactgct
     3481 gagcagaatg ccttataaac ctgcagggtc actcatctaa agtctagtga ccttaaactg
     3541 aatgatttaa aaaagaaaag aaagaaaaaa gaaactattt attctcgata ttttgttttg
     3601 cacagcaaag gcagctgctg acttctggaa gatcaatcaa tgcgacttaa agtgattcag
     3661 tgaaaacaaa aaacttggtg ggctgaaggc atcttccagt ttaccccacc ttagggtatg
     3721 ggtgggtgag aagggcagtt gagatggcag cattgatatg aatgaacact ccatagaaac
     3781 tgaattctct tttgtacaag atcacctgac atgattggga acagttgctt ttaattacag
     3841 atttaatttt tttcttcgtt aaagttttat gtaatttaac cctttgaaga cagaagtagt
     3901 tggatgaaat gcacagtcaa ttattataga aactgataac agggagtact tgttccccct
     3961 tttgccttct taagtacatt gtttaaaact agggaaaaag ggtatgtgta tattgtaaac
     4021 tatggatgtt aacactcaaa gaggttaagt cagtgaagta acctattcat caccagtacc
     4081 gctgtaccac taataaattg tttgccaaat ccttgtaata acatcttaat tttagacaat
     4141 catgtcactg tttttaatgt ttattttttt gtgtgtgttg cgtgtatcat gtatttattt
     4201 gttggcaaac tattgtttgt tgattaaaat agcactgttc cagtcagcca ctactttatg
     4261 acgtctgagg cacacccctt tccgaatttc aaggaccaag gtgacccgac ctgtgtatga
     4321 gagtgccaaa tggtgtttgg cttttcttaa cattcctttt tgtttgtttg ttttgttttc
     4381 cttcttaatg aactaaatac gaatagatgc aacttagttt ttgtaatact gaaatcgatt
     4441 caattgtata aacgattata atttctttca tggaagcatg attcttctga ttaaaaactg
     4501 tactccatat tttatgctgg ttgtctgcaa gcttgtgcga tgttatgttc atgttaatcc
     4561 tatttgtaaa atgaagtgtt cccaacctta tgttaaaaga gagaagtaaa taacagactg
     4621 tattcagtta ttttgccctt tattgaggaa ccagatttgt tttctttttg tttgtaatct
     4681 cattttgaaa taatcagcaa gttgaggtac tttcttcaaa tgctttgtac aatataaact
     4741 gttatgcctt tcagtgcatt actatgggag gagcaactaa aaaataaaga cttacaaaaa
     4801 gga
//
