LOCUS       NM_020947               5013 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens MTOR associated protein, eak-7 homolog (MEAK7), mRNA.
ACCESSION   NM_020947
VERSION     NM_020947.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Wang,L., Wu,D., Robinson,C.V. and Fu,T.M.
  TITLE     Identification of mEAK-7 as a human V-ATPase regulator via cryo-EM
            data mining
  JOURNAL   Proc Natl Acad Sci U S A 119 (35), e2203742119 (2022)
   PUBMED   35994636
  REMARK    GeneRIF: Identification of mEAK-7 as a human V-ATPase regulator via
            cryo-EM data mining.
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Wang,R., Qin,Y., Xie,X.S. and Li,X.
  TITLE     Molecular basis of mEAK7-mediated human V-ATPase regulation
  JOURNAL   Nat Commun 13 (1), 3272 (2022)
   PUBMED   35672408
  REMARK    GeneRIF: Molecular basis of mEAK7-mediated human V-ATPase
            regulation.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5013)
  AUTHORS   Yuan,P., Tang,C., Chen,B., Lei,P., Song,J., Xin,G., Wang,Z.,
            Hui,Y., Yao,W., Wang,G. and Zhao,G.
  TITLE     miR-32-5p suppresses the proliferation and migration of pancreatic
            adenocarcinoma cells by targeting TLDC1
  JOURNAL   Mol Med Rep 24 (5) (2021)
   PUBMED   34468015
  REMARK    GeneRIF: miR325p suppresses the proliferation and migration of
            pancreatic adenocarcinoma cells by targeting TLDC1.
REFERENCE   4  (bases 1 to 5013)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5013)
  AUTHORS   Nguyen,J.T., Ray,C., Fox,A.L., Mendonca,D.B., Kim,J.K. and
            Krebsbach,P.H.
  TITLE     Mammalian EAK-7 activates alternative mTOR signaling to regulate
            cell proliferation and migration
  JOURNAL   Sci Adv 4 (5), eaao5838 (2018)
   PUBMED   29750193
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5013)
  AUTHORS   Finelli,M.J., Sanchez-Pulido,L., Liu,K.X., Davies,K.E. and
            Oliver,P.L.
  TITLE     The Evolutionarily Conserved Tre2/Bub2/Cdc16 (TBC), Lysin Motif
            (LysM), Domain Catalytic (TLDc) Domain Is Neuroprotective against
            Oxidative Stress
  JOURNAL   J Biol Chem 291 (6), 2751-2763 (2016)
   PUBMED   26668325
REFERENCE   7  (bases 1 to 5013)
  AUTHORS   Moriya,K., Nagatoshi,K., Noriyasu,Y., Okamura,T., Takamitsu,E.,
            Suzuki,T. and Utsumi,T.
  TITLE     Protein N-myristoylation plays a critical role in the endoplasmic
            reticulum morphological change induced by overexpression of protein
            Lunapark, an integral membrane protein of the endoplasmic reticulum
  JOURNAL   PLoS One 8 (11), e78235 (2013)
   PUBMED   24223779
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 5013)
  AUTHORS   Venkatesan,K., Rual,J.F., Vazquez,A., Stelzl,U., Lemmens,I.,
            Hirozane-Kishikawa,T., Hao,T., Zenkner,M., Xin,X., Goh,K.I.,
            Yildirim,M.A., Simonis,N., Heinzmann,K., Gebreab,F., Sahalie,J.M.,
            Cevik,S., Simon,C., de Smet,A.S., Dann,E., Smolyar,A.,
            Vinayagam,A., Yu,H., Szeto,D., Borick,H., Dricot,A., Klitgord,N.,
            Murray,R.R., Lin,C., Lalowski,M., Timm,J., Rau,K., Boone,C.,
            Braun,P., Cusick,M.E., Roth,F.P., Hill,D.E., Tavernier,J.,
            Wanker,E.E., Barabasi,A.L. and Vidal,M.
  TITLE     An empirical framework for binary interactome mapping
  JOURNAL   Nat Methods 6 (1), 83-90 (2009)
   PUBMED   19060904
REFERENCE   9  (bases 1 to 5013)
  AUTHORS   Schroder,B., Wrocklage,C., Pan,C., Jager,R., Kosters,B.,
            Schafer,H., Elsasser,H.P., Mann,M. and Hasilik,A.
  TITLE     Integral and associated lysosomal membrane proteins
  JOURNAL   Traffic 8 (12), 1676-1686 (2007)
   PUBMED   17897319
REFERENCE   10 (bases 1 to 5013)
  AUTHORS   Wang,A.G., Yoon,S.Y., Oh,J.H., Jeon,Y.J., Kim,M., Kim,J.M.,
            Byun,S.S., Yang,J.O., Kim,J.H., Kim,D.G., Yeom,Y.I., Yoo,H.S.,
            Kim,Y.S. and Kim,N.S.
  TITLE     Identification of intrahepatic cholangiocarcinoma related genes by
            comparison with normal liver tissues using expressed sequence tags
  JOURNAL   Biochem Biophys Res Commun 345 (3), 1022-1032 (2006)
   PUBMED   16712791
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK310887.1, AC022165.9 and AK291237.1.
            
            On Nov 23, 2018 this sequence version replaced NM_020947.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.68721.1,
                                           SRR1803616.245219.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000343629.11/ ENSP00000343635.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-741               AK310887.1         100-840
            742-742             AC022165.9         131027-131027       c
            743-882             AK310887.1         842-981
            883-883             AC022165.9         130886-130886       c
            884-1175            AK310887.1         983-1274
            1176-1488           AK291237.1         1199-1511
            1489-5008           AC022165.9         120456-123975       c
            5009-5013           AC022165.9         120451-120455       c
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q24.1"
     gene            1..5013
                     /gene="MEAK7"
                     /gene_synonym="EAK7; KIAA1609; mEAK-7; TLDC1"
                     /note="MTOR associated protein, eak-7 homolog"
                     /db_xref="GeneID:57707"
                     /db_xref="HGNC:HGNC:29325"
                     /db_xref="MIM:619331"
     exon            1..59
                     /gene="MEAK7"
                     /gene_synonym="EAK7; KIAA1609; mEAK-7; TLDC1"
                     /inference="alignment:Splign:2.1.0"
     exon            60..237
                     /gene="MEAK7"
                     /gene_synonym="EAK7; KIAA1609; mEAK-7; TLDC1"
                     /inference="alignment:Splign:2.1.0"
     CDS             85..1455
                     /gene="MEAK7"
                     /gene_synonym="EAK7; KIAA1609; mEAK-7; TLDC1"
                     /EC_number="3.1.4.11"
                     /note="TLD domain containing 1; TBC/LysM-associated
                     domain-containing protein 1; TLD domain-containing protein
                     1; mammalian EAK-7; TBC/LysM-associated domain containing
                     1"
                     /codon_start=1
                     /product="MTOR-associated protein MEAK7"
                     /protein_id="NP_065998.3"
                     /db_xref="CCDS:CCDS32498.1"
                     /db_xref="GeneID:57707"
                     /db_xref="HGNC:HGNC:29325"
                     /db_xref="MIM:619331"
                     /translation="MGNSRSRVGRSFCSQFLPEEQAEIDQLFDALSSDKNSPNVSSKS
                     FSLKALQNHVGEALPPEMVTRLYDGMRRVDLTGKAKGPSENVSQEQFTASMSHLLKGN
                     SEEKSLMIMKMISATEGPVKAREVQKFTEDLVGSVVHVLSHRQELRGWTGKEAPGPNP
                     RVQVLAAQLLSDMKLQDGKRLLGPQWLDYDCDRAVIEDWVFRVPHVAIFLSVVICKGF
                     LILCSSLDLTTLVPERQVDQGRGFESILDVLSVMYINAQLPREQRHRWCLLFSSELHG
                     HSFSQLCGHITHRGPCVAVLEDHDKHVFGGFASCSWEVKPQFQGDNRCFLFSICPSMA
                     VYTHTGYNDHYMYLNHGQQTIPNGLGMGGQHNYFGLWVDVDFGKGHSRAKPTCTTYNS
                     PQLSAQENFQFDKMEVWAVGDPSEEQLAKGNKSILDADPEAQALLEISGHSRHSEGLR
                     EVPDDE"
     exon            238..468
                     /gene="MEAK7"
                     /gene_synonym="EAK7; KIAA1609; mEAK-7; TLDC1"
                     /inference="alignment:Splign:2.1.0"
     exon            469..613
                     /gene="MEAK7"
                     /gene_synonym="EAK7; KIAA1609; mEAK-7; TLDC1"
                     /inference="alignment:Splign:2.1.0"
     exon            614..1042
                     /gene="MEAK7"
                     /gene_synonym="EAK7; KIAA1609; mEAK-7; TLDC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1043..1161
                     /gene="MEAK7"
                     /gene_synonym="EAK7; KIAA1609; mEAK-7; TLDC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1162..1341
                     /gene="MEAK7"
                     /gene_synonym="EAK7; KIAA1609; mEAK-7; TLDC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1342..5013
                     /gene="MEAK7"
                     /gene_synonym="EAK7; KIAA1609; mEAK-7; TLDC1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1705..1710
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MEAK7"
                     /gene_synonym="EAK7; KIAA1609; mEAK-7; TLDC1"
                     /note="hexamer: AAGAAA"
     polyA_site      1729
                     /gene="MEAK7"
                     /gene_synonym="EAK7; KIAA1609; mEAK-7; TLDC1"
                     /note="major polyA site"
     regulatory      2913..2918
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MEAK7"
                     /gene_synonym="EAK7; KIAA1609; mEAK-7; TLDC1"
                     /note="hexamer: AGTAAA"
     polyA_site      2932
                     /gene="MEAK7"
                     /gene_synonym="EAK7; KIAA1609; mEAK-7; TLDC1"
     regulatory      3269..3274
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MEAK7"
                     /gene_synonym="EAK7; KIAA1609; mEAK-7; TLDC1"
                     /note="hexamer: ATTAAA"
     polyA_site      3295
                     /gene="MEAK7"
                     /gene_synonym="EAK7; KIAA1609; mEAK-7; TLDC1"
     regulatory      4989..4994
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MEAK7"
                     /gene_synonym="EAK7; KIAA1609; mEAK-7; TLDC1"
                     /note="hexamer: ATTAAA"
     polyA_site      5013
                     /gene="MEAK7"
                     /gene_synonym="EAK7; KIAA1609; mEAK-7; TLDC1"
                     /note="major polyA site"
ORIGIN      
        1 ctcgagagga gcccgtgggc tgctggaccg gaccggacag caccaggaag cccggcagga
       61 gaattctgcc agatatcagg acagatgggg aacagcagaa gccgtgtggg gcggagcttt
      121 tgttcacagt ttcttcctga ggaacaggca gagattgatc aattgtttga tgctctgtca
      181 tcagataaaa acagcccgaa tgtctcatcc aaatccttct ctctgaaggc actacagaac
      241 cacgtcgggg aagctcttcc cccagagatg gtcaccaggc tgtatgatgg catgcggagg
      301 gtcgacctga cagggaaggc gaagggaccc agtgagaacg tgtcccagga gcagttcaca
      361 gcatccatgt cccacctgtt gaaaggaaac tccgaggaga agagtctcat gattatgaaa
      421 atgatttctg ccacagaagg tcccgtgaag gccagagaag tccaaaagtt tacagaggat
      481 ctggttggct ctgtggtgca cgtgctaagc cacagacagg agctgagagg ctggactggg
      541 aaggaagccc cagggcccaa cccccgggtg caggtgctgg ctgctcagct gctctctgac
      601 atgaagctgc aagatggcaa gagacttctg gggccccagt ggctggacta tgactgtgac
      661 cgagctgtga tcgaggactg ggtgttcagg gtcccccatg tggccatatt cctgagtgtg
      721 gtcatttgca agggctttct catcctgtgc tcgtctcttg atctgactac cctggtccct
      781 gagcgtcaag tggaccaggg caggggtttt gagagcatcc tggatgtcct ctctgtcatg
      841 tacatcaacg cccagctgcc tcgggagcag cggcaccgct ggtgcctgct cttttcgtct
      901 gagctccatg gacacagctt ctcccagctc tgtggccaca tcactcaccg gggaccctgt
      961 gtggctgtcc tcgaggacca tgacaagcat gtgttcggtg ggtttgcctc ttgctcttgg
     1021 gaggtgaagc ctcagtttca aggggacaac agatgcttcc tgttctccat ctgccccagc
     1081 atggctgtgt acacacacac gggctacaac gaccactaca tgtacttgaa ccatggacag
     1141 cagacgatcc cgaacggact gggtatgggg gggcagcaca attactttgg gctttgggtg
     1201 gatgttgatt ttgggaaagg acacagcaga gccaagccca cgtgcaccac gtacaacagc
     1261 ccgcagctgt cggctcagga gaacttccag tttgataaga tggaggtgtg ggcggttgga
     1321 gacccctcag aggagcagtt ggccaagggc aacaagagca tcctggatgc ggaccctgag
     1381 gcccaggccc tgctggagat cagtgggcat tcgcgccaca gcgaagggct ccgggaagtc
     1441 ccggacgatg aatgaggagc cgcctgagcc ttcctggagc caggacctgg attcctctgg
     1501 gtagagggca acgcctgcag cccctcttcc ctcccacatg gtttaactgt aatagcgtac
     1561 cgcacgggtc acagcctggt agtcccacat gtgccaagaa gacgccctct gaagccatgt
     1621 cctgactcat gggtggatct cccagaaata gatgtttttt aagttttctt tttttcccta
     1681 cagggtcacc ctcaggatct ctccaagaaa tcttgtgtca aattagtaac tcagtcttat
     1741 actttgttct tttttaccaa cgaaatccta agcagctgag acctttcaga cccccgctgg
     1801 cattagggaa ttccgctggc attagggaat tccgctggca ttagggaatt ccgctggcat
     1861 tagggaattc cgctggcatt agggaattcc gctggcatta gggaattccg ctggcattag
     1921 ggaattccgc tggcattagg gaattccgct ggcattaggg aattccgctg gcattagcat
     1981 tgctgggatc tcaacttctc tattgaggag ggggttcctc ccgctggggt tcatatggcc
     2041 tcactcaggc catccctgtt agaaggtggt acctgatggt ctgttctgac gggtgaggga
     2101 cagaggcatc tggatgaaaa tctcgacgct ttctgggaac agcttccaat ggcatgggag
     2161 ttctgtattt aaaagcatga actgtgtata aaatggggaa atggaggcag gagactttga
     2221 ggtaacatgg tctggtggtg gcatgagtcc tcagaaggta tttgtaataa ggttccacac
     2281 ccagctctct cagctgtttt taaatgaatg tgtgtgagga acagatggga aagttgggag
     2341 atctgtctac agagaagcaa agttgtggtt ctcttgctaa cttcaaggtg agggacattg
     2401 ggcaccctaa gtttgggaac ttggttgata aatacgtata tggtccattc cataaatcag
     2461 tggtgagtga ctggcctggg ttctagacct ctgggaacca gcacctgagt cacagctgtc
     2521 taggcctcgg tgctggcctg ggttctagat ctctgggaac cagtgcctga gtcacagctg
     2581 tcagtgcagc catttgccca gggctgctcc cgagggggat gatgggaaat tcagcagtgt
     2641 agactgactt taaacaagct ccggtgatcc tgaaatgctg aagatcgtgt aggtgggttg
     2701 tggggtcagc agagctgcca tctgcccacg tctggaaaac aacacacggt gagtcaccgg
     2761 ttggccatga gatctcccca cttaaaggtg ctgtgagctt gtctctaaga tatatacctc
     2821 ttccttttgt cttttgctgt aagctttgac cttttgcaga tctgatgaaa atacaacctc
     2881 ttattgtata gtttgccttg attataagcc atagtaaatc gagctgttcg catttttgca
     2941 ggccttgcat tttctactgg gaggttcatc aaaccttcca cttagcaata gccctgactc
     3001 aggcagaatg cctcataaat tagccttcga aagaaaagtg cacgctcaga cagtgtgtgg
     3061 aggggcagga gcgtgatgca gacaaggact tagtcatgac tgattttcct tagccagttt
     3121 gtaatggctt atggggaaca ttgtgagctt cctacctgac atgttggcag aagtagaata
     3181 ttaggatcct caagcatctc taccgtaagt ccaaagaatt taggtcccta ttgtcacttt
     3241 gttttgcaat gttttttata aaaatgcaat taaatacctc actattaaac tgccatgatg
     3301 cctcagttaa aaaaaaaatt aaatgtccgt cagtgtctgt gccttggtga ccattgattg
     3361 ctgcttctgc ccaggccgct gcaaaggtta ggactttgtc cactcacatg ctttccaccc
     3421 tgagcttgtt ctaaggagag tgcagatcac gaatgcacct gcttccaggc agtgcactta
     3481 gagaaaggtc actggcccgg gcttgggagc ctgggggatg cagctctctc tgtgggcttc
     3541 tcttggtgca cggttctgcc tctggacact caaacctttt tagggcgtgt gcagggctgg
     3601 ggccagtgga cctgacagta ccttacctgg ctctgagaaa ctcgcaatcc tagtttgcac
     3661 aaatgaatag tttgttcttt tgtgaaacca gaatgagaat tcaggttcta aggactagag
     3721 gtctccctac aaatgaagac ttatcagggc cctagaaagt gctaggagga aggggttaaa
     3781 aaaaaaattt gtccgcgttg caaactatta taaatgattg ccacattgca agctattatt
     3841 ttctcccacc atatttttta attaaaaaaa agtctggggc cgggtgcggt ggctcacgcc
     3901 tgtaatccca gcactttggg aggccgaggt aggcggatca cctgaggtcg gaagttcgag
     3961 accagcctga ccaacatgga gaaaccccat ctctactaaa aatacaaaat tagctgggca
     4021 tggtggcaca tgcctgtaat ctcagctact caggaggctg aggtagtaga attgcttgaa
     4081 ccagggaggc agaggttgtg gtgagccgag atcgtgccat tgcactctag cctgggcaac
     4141 aagagtgaaa ctccgtttca agaaaaaaaa aaaggctggg tgtgatggct cacgcctgta
     4201 atcccagcat tttgggagct tgaggcaggc cgattgcttg agcccaggag ttcaagacca
     4261 gcctgggcaa catggcgaaa acccatctct acaaaaaaaa atacgaaaat taaccaggca
     4321 tggtaacaca tgcctgtggt cctagctact tgggaggctg aggtgtgagg atcatttagg
     4381 cctggaaggt tgaggctgct ataagctgtg attgtgccac tgcactccag cctgggcaac
     4441 acagcaagac cctatgttta aaaaaaattg tgtacctggt ttagaaaaaa aagataaaag
     4501 gatacaactt aaagtcagtc atcttcctgc ctcttctcca gctattttcc ctcctgacag
     4561 gcaaaggcac tgatgcaaaa atcactcagg tctcctccct gtgacttggt gcacacctgg
     4621 gcatcactgt cttttacaca ctgatgtgtc cctcgctttc ttttttaaag ggaggtccgt
     4681 aaccacccac ctgcctccct cttgtcttta tggactgcgt gatgtcccac catatggatg
     4741 ctctcgggcc tagcgttcca ccgtctgcag ctggggaaca ttttgtttgc agctaatcat
     4801 tgtcatgaat atcgtttatg tgtgccttgc tgtgatggtg tctgtgggat aaactcttag
     4861 aattggaatt gttggattga gaggtatatg ccttttattt agatatagat gaattaaaaa
     4921 aacctcttga gctcaattta tactcaatat accggggtgc ctgtttgcca tgttttcagc
     4981 aaaactgtat taaaatatgt ttttttaacc tga
//
