LOCUS       NM_005586               1606 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens MyoD family inhibitor (MDFI), transcript variant 3,
            mRNA.
ACCESSION   NM_005586 XM_005249116
VERSION     NM_005586.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,D., Liu,S., Liu,K., Kong,L., Xiao,L., Xin,Q., Jiang,C. and Wu,J.
  TITLE     MDFI promotes the proliferation and tolerance to chemotherapy of
            colorectal cancer cells by binding ITGB4/LAMB3 to activate the AKT
            signaling pathway
  JOURNAL   Cancer Biol Ther 25 (1), 2314324 (2024)
   PUBMED   38375821
  REMARK    GeneRIF: MDFI promotes the proliferation and tolerance to
            chemotherapy of colorectal cancer cells by binding ITGB4/LAMB3 to
            activate the AKT signaling pathway.
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Asai,M., Koike,Y., Kuwatsuka,Y., Yagi,Y., Kashiyama,K., Tanaka,K.,
            Mishima,H., Yoshiura,K., Utani,A. and Murota,H.
  TITLE     Multifaceted array-based keloidal gene expression profiling reveals
            specific MDFI upregulation in keloid lesions
  JOURNAL   Clin Exp Dermatol 46 (7), 1255-1261 (2021)
   PUBMED   33899950
  REMARK    GeneRIF: Multifaceted array-based keloidal gene expression
            profiling reveals specific MDFI upregulation in keloid lesions.
REFERENCE   3  (bases 1 to 1606)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 1606)
  AUTHORS   Sui,Y., Li,X., Oh,S., Zhang,B., Freeman,W.M., Shin,S. and
            Janknecht,R.
  TITLE     Opposite Roles of the JMJD1A Interaction Partners MDFI and MDFIC in
            Colorectal Cancer
  JOURNAL   Sci Rep 10 (1), 8710 (2020)
   PUBMED   32457453
  REMARK    GeneRIF: Opposite Roles of the JMJD1A Interaction Partners MDFI and
            MDFIC in Colorectal Cancer.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1606)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 1606)
  AUTHORS   Wistow,G., Bernstein,S.L., Wyatt,M.K., Fariss,R.N., Behal,A.,
            Touchman,J.W., Bouffard,G., Smith,D. and Peterson,K.
  TITLE     Expressed sequence tag analysis of human RPE/choroid for the
            NEIBank Project: over 6000 non-redundant transcripts, novel genes
            and splice variants
  JOURNAL   Mol Vis 8, 205-220 (2002)
   PUBMED   12107410
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 1606)
  AUTHORS   Snider,L., Thirlwell,H., Miller,J.R., Moon,R.T., Groudine,M. and
            Tapscott,S.J.
  TITLE     Inhibition of Tcf3 binding by I-mfa domain proteins
  JOURNAL   Mol Cell Biol 21 (5), 1866-1873 (2001)
   PUBMED   11238923
REFERENCE   8  (bases 1 to 1606)
  AUTHORS   Kraut,N., Snider,L., Chen,C.M., Tapscott,S.J. and Groudine,M.
  TITLE     Requirement of the mouse I-mfa gene for placental development and
            skeletal patterning
  JOURNAL   EMBO J 17 (21), 6276-6288 (1998)
   PUBMED   9799236
REFERENCE   9  (bases 1 to 1606)
  AUTHORS   Kraut,N.
  TITLE     The gene encoding I-mf (Mdfi) maps to human chromosome 6p21 and
            mouse chromosome 17
  JOURNAL   Mamm Genome 8 (8), 618-619 (1997)
   PUBMED   9250874
REFERENCE   10 (bases 1 to 1606)
  AUTHORS   Chen,C.M., Kraut,N., Groudine,M. and Weintraub,H.
  TITLE     I-mf, a novel myogenic repressor, interacts with members of the
            MyoD family
  JOURNAL   Cell 86 (5), 731-741 (1996)
   PUBMED   8797820
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CA393148.1 and BC007836.2.
            
            On Jun 2, 2019 this sequence version replaced NM_005586.3.
            
            Summary: This protein is a transcription factor that negatively
            regulates other myogenic family proteins. Studies of the mouse
            homolog, I-mf, show that it interferes with myogenic factor
            function by masking nuclear localization signals and preventing DNA
            binding. Knockout mouse studies show defects in the formation of
            vertebrae and ribs that also involve cartilage formation in these
            structures. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (3) differs in the 5' UTR,
            compared to variant 1. It encodes the longer isoform (1). Variants
            1, 3, and 4 encode the same isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC007836.2, U78313.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000230321.11/ ENSP00000230321.6
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-40                CA393148.1         3-42
            41-1606             BC007836.2         1-1566
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p21.1"
     gene            1..1606
                     /gene="MDFI"
                     /gene_synonym="I-MF; I-mfa"
                     /note="MyoD family inhibitor"
                     /db_xref="GeneID:4188"
                     /db_xref="HGNC:HGNC:6967"
                     /db_xref="MIM:604971"
     exon            1..185
                     /gene="MDFI"
                     /gene_synonym="I-MF; I-mfa"
                     /inference="alignment:Splign:2.1.0"
     exon            186..272
                     /gene="MDFI"
                     /gene_synonym="I-MF; I-mfa"
                     /inference="alignment:Splign:2.1.0"
     CDS             197..937
                     /gene="MDFI"
                     /gene_synonym="I-MF; I-mfa"
                     /note="isoform 1 is encoded by transcript variant 3;
                     inhibitor of MyoD family a; myogenic repressor I-mf"
                     /codon_start=1
                     /product="myoD family inhibitor isoform 1"
                     /protein_id="NP_005577.1"
                     /db_xref="CCDS:CCDS4857.1"
                     /db_xref="GeneID:4188"
                     /db_xref="HGNC:HGNC:6967"
                     /db_xref="MIM:604971"
                     /translation="MYQVSGQRPSGCDAPYGAPSAAPGPAQTLSLLPGLEVVTGSTHP
                     AEAAPEEGSLEEAATPMPQGNGPGIPQGLDSTDLDVPTEAVTCQPQGNPLGCTPLLPN
                     DSGHPSELGGTRRAGNGALGGPKAHRKLQTHPSLASQGSKKSKSSSKSTTSQIPLQAQ
                     EDCCVHCILSCLFCEFLTLCNIVLDCATCGSCSSEDSCLCCCCCGSGECADCDLPCDL
                     DCGILDACCESADCLEICMECCGLCFSS"
     exon            273..455
                     /gene="MDFI"
                     /gene_synonym="I-MF; I-mfa"
                     /inference="alignment:Splign:2.1.0"
     exon            456..680
                     /gene="MDFI"
                     /gene_synonym="I-MF; I-mfa"
                     /inference="alignment:Splign:2.1.0"
     exon            681..1606
                     /gene="MDFI"
                     /gene_synonym="I-MF; I-mfa"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1584..1589
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MDFI"
                     /gene_synonym="I-MF; I-mfa"
                     /note="hexamer: AATAAA"
     polyA_site      1606
                     /gene="MDFI"
                     /gene_synonym="I-MF; I-mfa"
                     /note="major polyA site"
ORIGIN      
        1 agaaggaagg ggcgaacggc gtgagctggc gccgaaatgg gagaaagcag cgagtgagag
       61 gggaaggggc gccaggcgag cacccgggag ccagcgggac ctgggcaggg gcgcccggag
      121 caggcgcgca tggcgggccc cgcgcgggga tccggctgga agagagcgta gcacggctcg
      181 cacgagtccg gggccgatgt accaggtgag cggccagcgc ccctctggct gcgacgcgcc
      241 ctatggagcc cccagcgcag ccccgggccc agcccagacc ctatccctcc ttcctgggct
      301 ggaggtagta acaggatcca ctcaccctgc ggaggcagca ccagaggagg gctccctgga
      361 ggaggcggca acccccatgc cccaaggcaa tggccctggc atcccccagg gcctggacag
      421 cactgacctc gacgtcccca cagaagctgt gacatgccag cctcagggga accccttggg
      481 ctgcacccca cttctgccga atgactctgg ccacccctca gagctgggcg gcaccagacg
      541 ggcggggaat ggtgccctgg gtggccccaa ggcccaccgg aagttgcaga cacacccatc
      601 tctcgccagc cagggcagca agaagagtaa gagcagcagc aaatccacca cctcccagat
      661 ccccctccag gcacaggaag actgctgtgt ccactgcatc ctgtcctgcc tgttctgcga
      721 gttcctgacg ctgtgcaaca tcgtcctgga ctgcgccacc tgtggctcct gcagctcgga
      781 ggactcgtgc ctctgctgct gctgctgtgg ctctggcgag tgtgccgact gcgacctgcc
      841 ctgcgacctg gactgcggca tcctggatgc ctgctgcgag tccgcggact gcctggagat
      901 ctgcatggag tgctgtgggc tctgcttctc ctcctgagcc tctgtcgggg gctaagccag
      961 cctggcgccc ctgcagattc cagcagggtc cctctgagtg gggccaggcc caggactgtc
     1021 acacaaggct tgagaagccc cctctccctg gtcctctcct acccacccat gtcctctcag
     1081 aaccccagcc ttgaaaatag tggggggcac tcagaggggc cacctcctca gccgtgggtg
     1141 gtgggcccat ggcagagaag cctgaactct ttactgggtt accaggttca tacattgctg
     1201 aggacctgac aggacaacct aggggcaggg ctggggtggg gaccgcaggg ggcagccagg
     1261 gctggggaac actgtgaaag ttacttgggg agggtgggcc ggtggggccg tagctctcta
     1321 cctctccctg ctcctggtgc ctgcctctct cctccacccc aggcttagag gacagaaaaa
     1381 tgtgaagaga cgccccaccc accctcagcc agccctctcc agtctccttt cctaggcttt
     1441 tttgggggcc taacccacgc agtgacccca gagggcaggg ctaggcgaga gcctggggtg
     1501 gggcgggagg gggaacagta tggaaaagac tggaagggga aaggaaggga agggagggag
     1561 ggtctgttct atctgttgct gtaaataaag atatttgtcc atctca
//
