LOCUS       NM_014641               6968 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), mRNA.
ACCESSION   NM_014641 XM_376479
VERSION     NM_014641.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6968)
  AUTHORS   Lafontaine,N., Shore,C.J., Campbell,P.J., Mullin,B.H., Brown,S.J.,
            Panicker,V., Dudbridge,F., Brix,T.H., Hegedus,L., Wilson,S.G.,
            Bell,J.T. and Walsh,J.P.
  TITLE     Epigenome-wide Association Study Shows Differential DNA Methylation
            of MDC1, KLF9, and CUTA in Autoimmune Thyroid Disease
  JOURNAL   J Clin Endocrinol Metab 109 (4), 992-999 (2024)
   PUBMED   37962983
  REMARK    GeneRIF: Epigenome-wide Association Study Shows Differential DNA
            Methylation of MDC1, KLF9, and CUTA in Autoimmune Thyroid Disease.
REFERENCE   2  (bases 1 to 6968)
  AUTHORS   Huang,C., Wang,L., Chen,H., Fu,W., Shao,L., Zhou,D., Wu,J. and
            Ye,Y.
  TITLE     A positive feedback loop between ID3 and PPARgamma via DNA damage
            repair regulates the efficacy of radiotherapy for rectal cancer
  JOURNAL   BMC Cancer 23 (1), 429 (2023)
   PUBMED   37170184
  REMARK    GeneRIF: A positive feedback loop between ID3 and PPARgamma via DNA
            damage repair regulates the efficacy of radiotherapy for rectal
            cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6968)
  AUTHORS   Pappas,G., Munk,S.H.N., Watanabe,K., Thomas,Q., Gal,Z., Gram,H.H.,
            Lee,M., Gomez-Cabello,D., Kanellis,D.C., Olivares-Chauvet,P.,
            Larsen,D.H., Gregersen,L.H., Maya-Mendoza,A., Galanos,P. and
            Bartek,J.
  TITLE     MDC1 maintains active elongation complexes of RNA polymerase II
  JOURNAL   Cell Rep 42 (1), 111979 (2023)
   PUBMED   36640322
  REMARK    GeneRIF: MDC1 maintains active elongation complexes of RNA
            polymerase II.
REFERENCE   4  (bases 1 to 6968)
  AUTHORS   Liang,Y., Qin,Y., Jiang,G., Feng,W. and Yuan,Y.
  TITLE     Targeting MDC1 promotes apoptosis and sensitizes Imatinib
            resistance in CML cells by mainly disrupting non-homologous
            end-joining repair
  JOURNAL   Med Oncol 39 (12), 226 (2022)
   PUBMED   36175703
  REMARK    GeneRIF: Targeting MDC1 promotes apoptosis and sensitizes Imatinib
            resistance in CML cells by mainly disrupting non-homologous
            end-joining repair.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6968)
  AUTHORS   Xie,R., Yan,Z., Jing,J., Wang,Y., Zhang,J., Li,Y., Liu,X., Yu,X.
            and Wu,C.
  TITLE     Functional defects of cancer-associated MDC1 mutations in DNA
            damage repair
  JOURNAL   DNA Repair (Amst) 114, 103330 (2022)
   PUBMED   35378404
  REMARK    GeneRIF: Functional defects of cancer-associated MDC1 mutations in
            DNA damage repair.
REFERENCE   6  (bases 1 to 6968)
  AUTHORS   Peng,A. and Chen,P.L.
  TITLE     NFBD1, like 53BP1, is an early and redundant transducer mediating
            Chk2 phosphorylation in response to DNA damage
  JOURNAL   J Biol Chem 278 (11), 8873-8876 (2003)
   PUBMED   12551934
  REMARK    GeneRIF: NFBD1 is parallel to 53BP1 in regulating Chk2 and
            downstream of H2AX in the recruitment of repair and signaling
            proteins to sites of DNA damage in tumor cells
REFERENCE   7  (bases 1 to 6968)
  AUTHORS   Xu,X. and Stern,D.F.
  TITLE     NFBD1/KIAA0170 is a chromatin-associated protein involved in DNA
            damage signaling pathways
  JOURNAL   J Biol Chem 278 (10), 8795-8803 (2003)
   PUBMED   12499369
  REMARK    GeneRIF: role in DNA damage signaling pathways
REFERENCE   8  (bases 1 to 6968)
  AUTHORS   Shang,Y.L., Bodero,A.J. and Chen,P.L.
  TITLE     NFBD1, a novel nuclear protein with signature motifs of FHA and
            BRCT, and an internal 41-amino acid repeat sequence, is an early
            participant in DNA damage response
  JOURNAL   J Biol Chem 278 (8), 6323-6329 (2003)
   PUBMED   12475977
  REMARK    GeneRIF: This nuclear protein with signature motifs of FHA and
            BRCT, and an internal 41-amino acid repeat sequence, is an early
            participant in DNA damage response.
REFERENCE   9  (bases 1 to 6968)
  AUTHORS   Bakkenist,C.J. and Kastan,M.B.
  TITLE     DNA damage activates ATM through intermolecular autophosphorylation
            and dimer dissociation
  JOURNAL   Nature 421 (6922), 499-506 (2003)
   PUBMED   12556884
REFERENCE   10 (bases 1 to 6968)
  AUTHORS   Ozaki,T., Nagase,T., Ichimiya,S., Seki,N., Ohiri,M., Nomura,N.,
            Takada,N., Sakiyama,S., Weber,B.L. and Nakagawara,A.
  TITLE     NFBD1/KIAA0170 is a novel nuclear transcriptional transactivator
            with BRCT domain
  JOURNAL   DNA Cell Biol 19 (8), 475-485 (2000)
   PUBMED   10975465
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from EF177823.1, D79992.1,
            AL662797.7 and BU621433.1.
            
            On Nov 22, 2018 this sequence version replaced NM_014641.2.
            
            Summary: The protein encoded by this gene contains an N-terminal
            forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central
            domain with 13 repetitions of an approximately 41-amino acid
            sequence. The encoded protein is required to activate the intra-S
            phase and G2/M phase cell cycle checkpoints in response to DNA
            damage. This nuclear protein interacts with phosphorylated histone
            H2AX near sites of DNA double-strand breaks through its BRCT
            motifs, and facilitates recruitment of the ATM kinase and meiotic
            recombination 11 protein complex to DNA damage foci. [provided by
            RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D79992.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000376406.8/ ENSP00000365588.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-27                EF177823.1         643-669
            28-1647             D79992.1           1-1620
            1648-1648           AL662797.7         35419-35419         c
            1649-3160           D79992.1           1622-3133
            3161-4674           AL662797.7         27634-29147         c
            4675-6967           D79992.1           4648-6940
            6968-6968           BU621433.1         18-18               c
FEATURES             Location/Qualifiers
     source          1..6968
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p21.33"
     gene            1..6968
                     /gene="MDC1"
                     /gene_synonym="NFBD1"
                     /note="mediator of DNA damage checkpoint 1"
                     /db_xref="GeneID:9656"
                     /db_xref="HGNC:HGNC:21163"
                     /db_xref="MIM:607593"
     exon            1..37
                     /gene="MDC1"
                     /gene_synonym="NFBD1"
                     /inference="alignment:Splign:2.1.0"
     exon            38..176
                     /gene="MDC1"
                     /gene_synonym="NFBD1"
                     /inference="alignment:Splign:2.1.0"
     CDS             41..6310
                     /gene="MDC1"
                     /gene_synonym="NFBD1"
                     /note="nuclear factor with BRCT domains 1; homologue to
                     Drosophila photoreceptor protein calphotin"
                     /codon_start=1
                     /product="mediator of DNA damage checkpoint protein 1"
                     /protein_id="NP_055456.2"
                     /db_xref="CCDS:CCDS34384.1"
                     /db_xref="GeneID:9656"
                     /db_xref="HGNC:HGNC:21163"
                     /db_xref="MIM:607593"
                     /translation="MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPE
                     KDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILR
                     PPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQ
                     RLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNS
                     DTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKR
                     GAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAE
                     EERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLE
                     KSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTE
                     RDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGI
                     HLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVS
                     LEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVG
                     AQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTK
                     DSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTT
                     GTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTE
                     PMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGK
                     QDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKA
                     FEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVG
                     AGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGD
                     QESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQE
                     APEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQAT
                     RSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVP
                     TALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTS
                     EPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVK
                     TPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTS
                     TEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGR
                     TNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAP
                     ELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPT
                     SRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPE
                     PVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQ
                     SVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRA
                     AGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIH
                     ASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEE
                     EEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGV
                     VDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRK
                     AGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGE
                     IISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEA
                     KPEAFVLSPLEMSST"
     exon            177..557
                     /gene="MDC1"
                     /gene_synonym="NFBD1"
                     /inference="alignment:Splign:2.1.0"
     exon            558..627
                     /gene="MDC1"
                     /gene_synonym="NFBD1"
                     /inference="alignment:Splign:2.1.0"
     exon            628..2108
                     /gene="MDC1"
                     /gene_synonym="NFBD1"
                     /inference="alignment:Splign:2.1.0"
     exon            2109..2168
                     /gene="MDC1"
                     /gene_synonym="NFBD1"
                     /inference="alignment:Splign:2.1.0"
     exon            2169..2261
                     /gene="MDC1"
                     /gene_synonym="NFBD1"
                     /inference="alignment:Splign:2.1.0"
     exon            2262..3053
                     /gene="MDC1"
                     /gene_synonym="NFBD1"
                     /inference="alignment:Splign:2.1.0"
     exon            3054..3124
                     /gene="MDC1"
                     /gene_synonym="NFBD1"
                     /inference="alignment:Splign:2.1.0"
     exon            3125..5602
                     /gene="MDC1"
                     /gene_synonym="NFBD1"
                     /inference="alignment:Splign:2.1.0"
     exon            5603..5722
                     /gene="MDC1"
                     /gene_synonym="NFBD1"
                     /inference="alignment:Splign:2.1.0"
     exon            5723..5905
                     /gene="MDC1"
                     /gene_synonym="NFBD1"
                     /inference="alignment:Splign:2.1.0"
     exon            5906..6031
                     /gene="MDC1"
                     /gene_synonym="NFBD1"
                     /inference="alignment:Splign:2.1.0"
     exon            6032..6142
                     /gene="MDC1"
                     /gene_synonym="NFBD1"
                     /inference="alignment:Splign:2.1.0"
     exon            6143..6968
                     /gene="MDC1"
                     /gene_synonym="NFBD1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6945..6950
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MDC1"
                     /gene_synonym="NFBD1"
                     /note="hexamer: AATAAA"
     polyA_site      6968
                     /gene="MDC1"
                     /gene_synonym="NFBD1"
                     /note="major polyA site"
ORIGIN      
        1 gaccgttact ggtggcgcgc gcggggactt aaagtagatc atggaggaca cccaggctat
       61 tgactgggat gttgaagaag aggaggagac agagcaatcc agtgaatcct tgaggtgtaa
      121 cgtggagcca gtagggcggc tacatatctt tagtggtgcc catggaccag aaaaagattt
      181 cccactacac ctcgggaaga atgtggtagg ccgaatgcct gactgctctg tggccctgcc
      241 ctttccatct atctccaaac aacatgcaga gattgaaatc ttagcctggg acaaggcacc
      301 tatcctccga gactgtggga gccttaatgg tactcaaatc ctgagacctc ctaaggtttt
      361 gagccctggg gtgagtcacc gtctgaggga ccaggaattg attctctttg ctgacttgct
      421 ctgccagtac catcgcctgg atgtctctct gccctttgtc tcccggggcc ctctgacagt
      481 agaagagaca cccagagtac agggagaaac tcaaccccag aggcttctgt tggctgagga
      541 ctcggaggag gaagtagatt ttctttctga aaggcgtatg gtaaaaaaat caaggaccac
      601 atcttcctct gtgatagttc cagagagtga tgaagagggg cattccccgg tcctgggcgg
      661 ccttgggccg ccttttgcct tcaatttgaa cagtgacaca gatgtggaag aaggtcagca
      721 accagccaca gaggaggcct cctcagctgc cagaagaggt gccactgtag aggcaaagca
      781 gtctgaagct gaagttgtaa ctgaaatcca gcttgaaaag gatcagcctt tagtgaagga
      841 gagggacaat gatacaaaag tcaagagggg tgcagggaat ggggtggttc cagctggggt
      901 gattctggag aggagccaac ctcctggaga ggacagtgac acagatgtgg atgatgacag
      961 caggcctcct ggaaggccag ctgaggtcca tttggaaagg gctcagcctt ttggcttcat
     1021 cgacagcgac actgatgcgg aagaagagag gatcccagca accccagttg tcattcctat
     1081 gaagaagagg aagatcttcc atggagtagg tacaaggggt cctggagcac caggcctggc
     1141 ccatctgcag gagagccagg ctggtagtga tacagatgtg gaagaaggca aggccccaca
     1201 ggctgtccct ctggagaaaa gccaagcttc catggttatc aacagcgata cagatgacga
     1261 ggaagaagtc tcagcagcgc tgactttggc acatctgaaa gagagccagc ctgctatatg
     1321 gaacagagat gcagaagagg acatgcccca acgtgtggtc cttctgcagc gaagccaaac
     1381 caccactgag agagacagtg acacagacgt ggaggaggaa gagctcccag tggaaaatag
     1441 agaagctgtc ctcaaggatc acacaaagat tagagccctt gttagagcac attcagaaaa
     1501 ggaccaacct ccttttgggg acagtgatga cagtgtggaa gcagataaga gctcacctgg
     1561 gatccacctg gagagaagcc aagcctccac cacagtggac atcaacacac aagtggagaa
     1621 ggaagtcccg ccagggtcag ccattataca tataaagaag catcaggtgt ctgtggaggg
     1681 gacaaatcaa acagatgtga aagcagttgg gggaccagca aagctgcttg tggtatctct
     1741 agaggaagcc tggcctctgc atggggactg tgaaacagat gcagaggagg gcacctccct
     1801 aacagcctca gtagttgcag atgtaagaaa gagccagctt ccagcagaag gggatgctgg
     1861 ggcagagtgg gctgcagctg ttcttaagca ggagagagct catgaggtgg gggcccaggg
     1921 tgggccacct gtggcacaag tggagcagga cctccctatc tcaagagaga acctcacaga
     1981 tctggtggtg gacacagaca ctctagggga atccacccag ccacagagag agggagccca
     2041 ggtccccaca ggaagggaga gagaacaaca tgtgggtggg accaaggact ctgaagacaa
     2101 ctatggtgat tctgaagatc tggacctaca agctacccag tgctttctgg agaatcaggg
     2161 cctggaagca gtccagagca tggaggatga acctacccag gccttcatgt tgactccacc
     2221 ccaagagctt ggcccttccc attgcagctt ccagacaaca ggtaccctag atgaaccatg
     2281 ggaggtcctg gctacacagc cattctgtct gagagagtct gaggactctg agacccagcc
     2341 ttttgacacg caccttgagg cctatggacc ttgcctgtct ccacctaggg caataccagg
     2401 agaccaacat ccagagagcc cagttcacac agagccaatg gggattcaag gcagagggag
     2461 gcagactgtg gataaagtca tgggtatacc aaaagaaaca gcagagaggg tgggccctga
     2521 gagagggcca ttggagagag aaactgagaa actgctacca gaaagacaga cagatgtgac
     2581 aggagaggaa gaattaacca aggggaaaca ggacagagaa caaaaacagt tgttagctag
     2641 agacacccag agacaagaat ctgacaaaaa tggggaaagt gcaagtcctg aaagagatag
     2701 ggagagtttg aaggtagaaa ttgagacatc tgaggaaata caagagaaac aagtacagaa
     2761 gcagaccctt ccaagcaaag catttgagag agaagtagag agaccagtag caaacagaga
     2821 gtgcgatcca gccgagttag aagagaaggt gcccaaagtg atcctggaga gagatacaca
     2881 gagaggggag ccagagggag ggagccagga ccagaaaggg caggcctcca gcccaacacc
     2941 agagcctggg gtgggggcgg gggaccttcc gggacctacc tcagcccccg taccttctgg
     3001 gagccagtca ggtggaaggg gatccccagt gagccccagg aggcatcaga aaggcctcct
     3061 gaattgcaag atgccacctg ctgagaaggc ttccaggatc agagctgctg agaaggtttc
     3121 caggggcgat caggaatctc cagatgcttg tctgcctcct acagtacctg aagccccagc
     3181 cccaccccaa aagcccctta actctcagag ccagaaacat cttgcacctc cgccccttct
     3241 ttctcccctt ttaccttcta tcaagccaac cgttcgtaag accaggcaag atgggagtca
     3301 ggaagctcca gaggctccct tgtcctcaga gctggagcct ttccacccaa agcctaaaat
     3361 tagaactcgg aagtcctcca gaatgacacc ctttccagct acctctgctg cccctgagcc
     3421 ccacccttcc acctccacag cccagccagt cactcccaag cccacatctc aggccactag
     3481 gagcaggaca aataggtcct ctgtcaagac ccctgaacca gttgtcccca cagcccctga
     3541 gctccagcct tccacctcca cagaccagcc tgtcacctct gagcccacat ctcaggttac
     3601 taggggaaga aaaagtagat cctctgtcaa gacccctgaa acagttgtgc ccacagccct
     3661 tgagctccag ccttccacct ccaccgaccg acctgtcacc tctgaaccca cctctcaggc
     3721 tactagggga agaaaaaata gatcctctgt caagacccct gaaccagttg tccccacagc
     3781 ccctgagctc cagccttcca cctccacaga ccagcctgtc acttctgagc ccacatatca
     3841 ggctactagg ggaagaaaaa atagatcctc tgtcaagacc cctgaaccag ttgtgcccac
     3901 agcccctgag ctccggcctt ccacctccac agaccgacct gtcaccccca agcccacatc
     3961 tcggaccact aggagcagga caaatatgtc ctctgtcaag acccctgaaa cagttgtccc
     4021 cacagcccct gagctccaga tttccacctc cacagaccaa cctgtcaccc ctaagcccac
     4081 atctcggacc actaggagca ggacaaatat gtcctctgtg aagaaccctg aatcaactgt
     4141 ccctatagcc cctgagctcc caccttccac ctccacagag cagcctgtca cccctgagcc
     4201 cacatctcgg gctactaggg gaagaaaaaa tagatcctct ggcaagaccc ctgaaacact
     4261 tgtccccaca gcccctaagc tcgagccttc cacttccaca gaccaacctg tcactcctga
     4321 gcccacatct caggccacca ggggcaggac aaataggtcc tctgtgaaga cccctgaaac
     4381 agttgtcccc acagcccctg agctccagcc ttccacctcc acagaccagc ctgttacccc
     4441 tgagcctacg tctcaggcta ctaggggaag aacagataga tcctctgtca agactcctga
     4501 aacagttgtc cccacagccc ctgagctaca ggcttccgcc tccacagacc agcctgtcac
     4561 ctctgagccc acatctcgga ccactagggg aagaaaaaat cggtcctctg tcaagacccc
     4621 tgaaacagtt gtgcccgcag cccctgagct ccagccttcc acctccacag accaacctgt
     4681 cacccctgag cccacatctc gggccactag gggcaggaca aataggtcct ctgtcaagac
     4741 ccctgaatca attgtcccta tagcccctga gcttcagcct tccacctcca gaaaccagct
     4801 tgtcacccct gagcccacat ctcgggccac taggtgcagg acaaataggt cctctgtcaa
     4861 gacccctgag ccagttgtcc ccacagcccc tgagccccat cctaccacct ccacagacca
     4921 gcctgtcacc cccaagctca catctagggc cactaggaga aagacaaata ggtcctctgt
     4981 caagactccc aaaccagttg aaccagcagc ctctgatctt gagcctttta cccccacaga
     5041 ccagtccgtc acccctgagg ccatagctca gggtggtcag agcaaaacac tgaggtcttc
     5101 cacagtaaga gctatgccgg ttcctaccac ccctgaattc caatctcctg tcaccacaga
     5161 ccagcctatt tcccctgagc ctattactca acccagttgc atcaagaggc agagagccgc
     5221 tgggaaccct ggctccctcg cagctcccat tgaccataag ccttgctctg cacccttgga
     5281 acctaaatcc caggcctcaa ggaaccaaag atggggagca gtgagagcag ctgaatccct
     5341 tacagccatt cctgagcctg cctctcccca gcttcttgag acaccaattc atgcctccca
     5401 gatccaaaag gtggaaccag caggtagatc taggttcacc ccggagctcc agcctaaggc
     5461 ctctcaaagc cgcaagaggt ctttagctac catggattca ccaccacatc aaaaacagcc
     5521 ccaaagaggg gaagtctccc agaagacagt gattatcaag gaagaggaag aagatactgc
     5581 agagaagcca gggaaggaag aggatgtcgt gactccaaaa ccaggcaaga gaaagagaga
     5641 ccaggcagag gaggagccca acagaatacc aagccgcagc ctccgacgga ccaaacttaa
     5701 ccaagaatca acagccccca aagtgctctt cacaggagtg gtggatgctc ggggagagcg
     5761 ggctgtgctg gcactggggg gaagtctggc tggttcagcg gcagaggctt cccacctggt
     5821 cactgatcgc atccgccgga cagtcaagtt cctgtgtgcc ctggggcggg gaatccccat
     5881 tctgtccctg gactggctgc atcagtcccg caaggctggt ttcttcttac ccccggatga
     5941 atatgtggtg accgaccctg agcaagagaa gaactttggc tttagccttc aagacgcact
     6001 gagcagggct cgggagcgaa ggctgctaga gggctatgag atctatgtga cccctggagt
     6061 ccagccacca ccacctcaga tgggagagat tattagctgc tgtggaggca catacctacc
     6121 cagcatgcct cggtcctata agcctcagag agttgtgatc acatgccctc aggacttccc
     6181 tcattgctcc attccactac gggttgggct gcccctcctc tcgcctgagt tcctgctgac
     6241 tggagtgctg aagcaggaag ccaagccaga ggcctttgtc ctctcccctt tggagatgtc
     6301 atccacctga gaactccact acccttttcc ctcccagacc acgaattaga agatatgtgg
     6361 aagaaagaac tcagggcgtt agaaaggatt ggggtatatt gatacaactt gtcctggaac
     6421 atgggtggga ccagaaatct ttatgaataa atgaaaagat aagggatttg gaagccacag
     6481 gttgtttttt gtttgtttgt ttgttttttt aatggccatt ttattttatt tgtatttata
     6541 gttttttatt tgtatagatt taggggatac aagatttctt acatgcatgt attaaatggc
     6601 cattttaaaa ttagctagtt tcatgctcag atgtcataag tggcagctat ctttagccag
     6661 actgttgcag ttattgctcg atgccactca tggtgtccta cctcctattt ggaaaccatc
     6721 tctatttttt tcttactgag attcttactt tggggtcagg aacttgaagg gatgcttgga
     6781 gtgagtagat ttgagggtcc agttatggag tgctactaaa acattttctt ctctcctggc
     6841 ctctggaagc atctttagct ttgactttgg gcaagtctct gtacttttct ggccagcttt
     6901 tccaggattt ataaaattag agcttcggct tgacctctgt gataaataaa tattcactct
     6961 gtgcctta
//
