LOCUS       NM_024596               8008 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens microcephalin 1 (MCPH1), transcript variant 1, mRNA.
ACCESSION   NM_024596
VERSION     NM_024596.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8008)
  AUTHORS   Caraffi,S.G., Pollazzon,M., Farooq,M., Fatima,A., Larsen,L.A.,
            Zuntini,R., Napoli,M. and Garavelli,L.
  TITLE     MCPH1: A Novel Case Report and a Review of the Literature
  JOURNAL   Genes (Basel) 13 (4), 634 (2022)
   PUBMED   35456440
  REMARK    GeneRIF: MCPH1: A Novel Case Report and a Review of the Literature.
            Review article
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8008)
  AUTHORS   Khan,N.M., Masoud,M.S., Baig,S.M., Qasim,M. and Chang,J.
  TITLE     Identification of Pathogenic Mutations in Primary Microcephaly-
            (MCPH-) Related Three Genes CENPJ, CASK, and MCPH1 in
            Consanguineous Pakistani Families
  JOURNAL   Biomed Res Int 2022, 3769948 (2022)
   PUBMED   35281599
  REMARK    GeneRIF: Identification of Pathogenic Mutations in Primary
            Microcephaly- (MCPH-) Related Three Genes CENPJ, CASK, and MCPH1 in
            Consanguineous Pakistani Families.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8008)
  AUTHORS   Kristofova,M. and Wang,Z.Q.
  TITLE     MCPH1, beyond its role deciding the brain size
  JOURNAL   Aging (Albany NY) 13 (20), 23437-23439 (2021)
   PUBMED   34705666
  REMARK    GeneRIF: MCPH1, beyond its role deciding the brain size.
REFERENCE   4  (bases 1 to 8008)
  AUTHORS   Gavvovidis,I., Rost,I., Trimborn,M., Kaiser,F.J., Purps,J.,
            Wiek,C., Hanenberg,H., Neitzel,H. and Schindler,D.
  TITLE     A novel MCPH1 isoform complements the defective chromosome
            condensation of human MCPH1-deficient cells
  JOURNAL   PLoS One 7 (8), e40387 (2012)
   PUBMED   22952573
  REMARK    GeneRIF: MCPH1 encodes different isoforms that are differentially
            regulated at the transcript level and have different functions at
            the protein level
REFERENCE   5  (bases 1 to 8008)
  AUTHORS   Wang,Y.Q. and Su,B.
  TITLE     Molecular evolution of microcephalin, a gene determining human
            brain size
  JOURNAL   Hum Mol Genet 13 (11), 1131-1137 (2004)
   PUBMED   15056608
REFERENCE   6  (bases 1 to 8008)
  AUTHORS   Kumar,A., Markandaya,M. and Girimaji,S.C.
  TITLE     Primary microcephaly: microcephalin and ASPM determine the size of
            the human brain
  JOURNAL   J Biosci 27 (7), 629-632 (2002)
   PUBMED   12571366
  REMARK    GeneRIF: microcephalin and ASPM determine the size of the human
            brain
REFERENCE   7  (bases 1 to 8008)
  AUTHORS   Jackson,A.P., Eastwood,H., Bell,S.M., Adu,J., Toomes,C., Carr,I.M.,
            Roberts,E., Hampshire,D.J., Crow,Y.J., Mighell,A.J., Karbani,G.,
            Jafri,H., Rashid,Y., Mueller,R.F., Markham,A.F. and Woods,C.G.
  TITLE     Identification of microcephalin, a protein implicated in
            determining the size of the human brain
  JOURNAL   Am J Hum Genet 71 (1), 136-142 (2002)
   PUBMED   12046007
  REMARK    GeneRIF: Identification of microcephalin, a protein implicated in
            determining the size of the human brain, which is mapped to the
            MCPH1 locus and is mutated in primary microcephaly.
REFERENCE   8  (bases 1 to 8008)
  AUTHORS   Neitzel,H., Neumann,L.M., Schindler,D., Wirges,A., Tonnies,H.,
            Trimborn,M., Krebsova,A., Richter,R. and Sperling,K.
  TITLE     Premature chromosome condensation in humans associated with
            microcephaly and mental retardation: a novel autosomal recessive
            condition
  JOURNAL   Am J Hum Genet 70 (4), 1015-1022 (2002)
   PUBMED   11857108
REFERENCE   9  (bases 1 to 8008)
  AUTHORS   Jackson,A.P., McHale,D.P., Campbell,D.A., Jafri,H., Rashid,Y.,
            Mannan,J., Karbani,G., Corry,P., Levene,M.I., Mueller,R.F.,
            Markham,A.F., Lench,N.J. and Woods,C.G.
  TITLE     Primary autosomal recessive microcephaly (MCPH1) maps to chromosome
            8p22-pter
  JOURNAL   Am J Hum Genet 63 (2), 541-546 (1998)
   PUBMED   9683597
REFERENCE   10 (bases 1 to 8008)
  AUTHORS   Verloes,A., Drunat,S., Gressens,P. and Passemard,S.
  TITLE     Primary Autosomal Recessive Microcephalies and Seckel Syndrome
            Spectrum Disorders - RETIRED CHAPTER, FOR HISTORICAL REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301772
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC016065.14, AC018398.10 and
            AF287957.6.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_024596.4.
            
            Summary: This gene encodes a DNA damage response protein. The
            encoded protein may play a role in G2/M checkpoint arrest via
            maintenance of inhibitory phosphorylation of cyclin-dependent
            kinase 1. Mutations in this gene have been associated with primary
            autosomal recessive microcephaly 1 and premature chromosome
            condensation syndrome. Alternatively spliced transcript variants
            have been described. [provided by RefSeq, Feb 2010].
            
            Transcript Variant: This variant (1, also known as MCPH1-FL)
            encodes isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.183559.1,
                                           SRR1660807.17759.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000344683.10/ ENSP00000342924.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-63                AC016065.14        87578-87640         c
            64-155              AC016065.14        84897-84988         c
            156-274             AC016065.14        79384-79502         c
            275-362             AC016065.14        62681-62768         c
            363-477             AC016065.14        58105-58219         c
            478-621             AC016065.14        55171-55314         c
            622-711             AC016065.14        52111-52200         c
            712-1866            AC016065.14        48720-49874         c
            1867-1976           AC016065.14        39015-39124         c
            1977-2014           AC016065.14        16636-16673         c
            2015-2177           AC016065.14        13391-13553         c
            2178-2255           AC018398.10        104802-104879       c
            2256-2493           AF287957.6         26947-27184
            2494-8008           AF287957.6         48487-54001
FEATURES             Location/Qualifiers
     source          1..8008
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p23.1"
     gene            1..8008
                     /gene="MCPH1"
                     /gene_synonym="BRIT1; MCT"
                     /note="microcephalin 1"
                     /db_xref="GeneID:79648"
                     /db_xref="HGNC:HGNC:6954"
                     /db_xref="MIM:607117"
     exon            1..63
                     /gene="MCPH1"
                     /gene_synonym="BRIT1; MCT"
                     /inference="alignment:Splign:2.1.0"
     CDS             42..2549
                     /gene="MCPH1"
                     /gene_synonym="BRIT1; MCT"
                     /note="isoform 1 is encoded by transcript variant 1;
                     BRCT-repeat inhibitor of TERT expression 1"
                     /codon_start=1
                     /product="microcephalin isoform 1"
                     /protein_id="NP_078872.3"
                     /db_xref="CCDS:CCDS43689.1"
                     /db_xref="GeneID:79648"
                     /db_xref="HGNC:HGNC:6954"
                     /db_xref="MIM:607117"
                     /translation="MAAPILKDVVAYVEVWSSNGTENYSKTFTTQLVDMGAKVSKTFN
                     KQVTHVIFKDGYQSTWDKAQKRGVKLVSVLWVEKCRTAGAHIDESLFPAANMNEHLSS
                     LIKKKRKCMQPKDFNFKTPENDKRFQKKFEKMAKELQRQKTNLDDDVPILLFESNGSL
                     IYTPTIEINSRHHSAMEKRLQEMKEKRENLSPTSSQMIQQSHDNPSNSLCEAPLNISR
                     DTLCSDEYFAGGLHSSFDDLCGNSGCGNQERKLEGSINDIKSDVCISSLVLKANNIHS
                     SPSFTHLDKSSPQKFLSNLSKEEINLQRNIAGKVVTPDQKQAAGMSQETFEEKYRLSP
                     TLSSTKGHLLIHSRPRSSSVKRKRVSHGSHSPPKEKCKRKRSTRRSIMPRLQLCRSED
                     RLQHVAGPALEALSCGESSYDDYFSPDNLKERYSENLPPESQLPSSPAQLSCRSLSKK
                     ERTSIFEMSDFSCVGKKTRTVDITNFTAKTISSPRKTGNGEGRATSSCVTSAPEEALR
                     CCRQAGKEDACPEGNGFSYTIEDPALPKGHDDDLTPLEGSLEEMKEAVGLKSTQNKGT
                     TSKISNSSEGEAQSEHEPCFIVDCNMETSTEEKENLPGGYSGSVKNRPTRHDVLDDSC
                     DGFKDLIKPHEELKKSGRGKKPTRTLVMTSMPSEKQNVVIQVVDKLKGFSIAPDVCET
                     TTHVLSGKPLRTLNVLLGIARGCWVLSYDWVLWSLELGHWISEEPFELSHHFPAAPLC
                     RSECHLSAGPYRGTLFADQPAMFVSPASSPPVAKLCELVHLCGGRVSQVPRQASIVIG
                     PYSGKKKATVKYLSEKWVLDSITQHKVCAPENYLLSQ"
     exon            64..155
                     /gene="MCPH1"
                     /gene_synonym="BRIT1; MCT"
                     /inference="alignment:Splign:2.1.0"
     exon            156..274
                     /gene="MCPH1"
                     /gene_synonym="BRIT1; MCT"
                     /inference="alignment:Splign:2.1.0"
     exon            275..362
                     /gene="MCPH1"
                     /gene_synonym="BRIT1; MCT"
                     /inference="alignment:Splign:2.1.0"
     exon            363..477
                     /gene="MCPH1"
                     /gene_synonym="BRIT1; MCT"
                     /inference="alignment:Splign:2.1.0"
     exon            478..621
                     /gene="MCPH1"
                     /gene_synonym="BRIT1; MCT"
                     /inference="alignment:Splign:2.1.0"
     exon            622..711
                     /gene="MCPH1"
                     /gene_synonym="BRIT1; MCT"
                     /inference="alignment:Splign:2.1.0"
     exon            712..1866
                     /gene="MCPH1"
                     /gene_synonym="BRIT1; MCT"
                     /inference="alignment:Splign:2.1.0"
     exon            1867..1976
                     /gene="MCPH1"
                     /gene_synonym="BRIT1; MCT"
                     /inference="alignment:Splign:2.1.0"
     exon            1977..2014
                     /gene="MCPH1"
                     /gene_synonym="BRIT1; MCT"
                     /inference="alignment:Splign:2.1.0"
     exon            2015..2177
                     /gene="MCPH1"
                     /gene_synonym="BRIT1; MCT"
                     /inference="alignment:Splign:2.1.0"
     exon            2178..2255
                     /gene="MCPH1"
                     /gene_synonym="BRIT1; MCT"
                     /inference="alignment:Splign:2.1.0"
     exon            2256..2493
                     /gene="MCPH1"
                     /gene_synonym="BRIT1; MCT"
                     /inference="alignment:Splign:2.1.0"
     exon            2494..8008
                     /gene="MCPH1"
                     /gene_synonym="BRIT1; MCT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3104..3109
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MCPH1"
                     /gene_synonym="BRIT1; MCT"
                     /note="hexamer: AATAAA"
     polyA_site      3123
                     /gene="MCPH1"
                     /gene_synonym="BRIT1; MCT"
                     /note="major polyA site"
     regulatory      3775..3780
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MCPH1"
                     /gene_synonym="BRIT1; MCT"
                     /note="hexamer: AATAAA"
     polyA_site      3793
                     /gene="MCPH1"
                     /gene_synonym="BRIT1; MCT"
     regulatory      6603..6608
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MCPH1"
                     /gene_synonym="BRIT1; MCT"
                     /note="hexamer: AATAAA"
     polyA_site      6622
                     /gene="MCPH1"
                     /gene_synonym="BRIT1; MCT"
     regulatory      7981..7986
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MCPH1"
                     /gene_synonym="BRIT1; MCT"
                     /note="hexamer: AATAAA"
     polyA_site      8008
                     /gene="MCPH1"
                     /gene_synonym="BRIT1; MCT"
ORIGIN      
        1 aagcaccgcg taggccagct ggccggatcc cgccgtctgt catggcggcc cccatcctga
       61 aagatgtagt ggcctatgtt gaagtgtggt catccaatgg aacagaaaat tattcaaaga
      121 catttacaac acagcttgtg gatatggggg caaaggtttc aaaaactttt aacaaacaag
      181 taactcacgt tatcttcaaa gatggctacc agagcacttg ggacaaagct cagaagagag
      241 gcgtaaagct cgtttcggtg ctctgggtgg aaaaatgcag gacagctgga gcacacattg
      301 atgaatcatt gttccctgca gctaatatga atgaacactt atcaagccta attaaaaaaa
      361 aacgtaaatg tatgcagccc aaagatttta attttaaaac accagaaaat gataagagat
      421 ttcagaagaa atttgagaaa atggctaaag agctacaaag gcaaaaaaca aatctagatg
      481 atgatgtacc tattctctta tttgaatcta atggttcatt aatatatact cccacaattg
      541 aaattaatag taggcaccac agcgcaatgg agaagagatt acaagagatg aaggagaaaa
      601 gggaaaatct ttcccccacc tcttcccaaa tgattcagca gtctcatgat aatccaagta
      661 actctctgtg tgaagcacct ttgaacattt cacgtgatac tttgtgttca gatgaatact
      721 ttgctggtgg cttacactca tcttttgatg atctttgtgg aaactcagga tgtggaaatc
      781 aggaaaggaa gttggaagga tccattaatg acattaaaag tgatgtgtgt atttcttcac
      841 ttgtattgaa agcaaataat attcattcat caccatcttt cactcacctc gataaatcaa
      901 gtcctcagaa atttctgagt aatctttcaa aggaagaaat aaacttgcaa agaaatattg
      961 caggtaaagt agtcacccct gaccaaaagc aggctgcagg tatgtctcag gagacgtttg
     1021 aagagaagta tcgtttgtct cctaccttat cttcaacaaa aggccacctt ttgatacatt
     1081 caagacccag gagttcctca gtaaagagaa aaagagtatc acatggctcc cattcacctc
     1141 cgaaggaaaa atgcaagaga aagaggagca ccaggagatc tatcatgccg aggctgcagc
     1201 tgtgcaggtc ggaagacagg ctgcagcacg tggcgggacc tgccctggag gctcttagct
     1261 gtggggagtc ttcatatgat gactattttt cacctgataa tcttaaggaa aggtattcag
     1321 agaatcttcc tcctgaatct cagctgccat caagccctgc tcagttgagc tgcagaagtc
     1381 tttctaagaa ggagagaaca agcatatttg aaatgtctga tttttcctgc gttggcaaaa
     1441 aaaccagaac agttgacatt accaatttca cagcaaaaac catctccagt cctcggaaaa
     1501 ctggaaatgg tgaaggccgt gcaacttcga gttgcgtgac ttctgcccct gaagaagccc
     1561 taaggtgttg tagacaggct gggaaagaag acgcatgccc agagggaaat ggcttttctt
     1621 acaccattga ggaccctgct cttccaaaag gacatgatga tgatttaact cctttggaag
     1681 gaagccttga agaaatgaaa gaagcggttg gtctgaaaag cacacagaac aaaggtacca
     1741 cttccaaaat atcaaactcc tctgaaggcg aagcccagag tgaacatgag ccatgtttta
     1801 tagttgactg taacatggag acgtctacag aagagaagga aaacttaccc ggaggataca
     1861 gtggaagtgt taaaaataga ccaacaaggc atgatgtttt agatgactca tgtgacggct
     1921 ttaaggacct catcaaacct catgaggaat tgaagaaaag tgggagaggc aaaaagccaa
     1981 caagaacatt agtcatgaca agcatgccat ctgaaaagca gaatgtcgtc atccaggttg
     2041 tggataaatt gaaaggcttt tcaattgcac cagacgtctg tgagaccacg actcacgtgc
     2101 tttccgggaa gccacttcgc accctgaatg tgctgctggg aattgcgcgt ggctgctggg
     2161 ttctctctta tgattgggtg ctatggtctt tagaattggg tcactggatt tctgaggagc
     2221 cgttcgaact gtctcaccac ttccctgcag ctcccctgtg ccgaagcgag tgccacttgt
     2281 ctgcagggcc gtaccgcgga accctctttg ccgaccagcc agcgatgttt gtctcgcctg
     2341 ccagcagccc cccagtggcc aagctctgtg aactagtcca cctgtgcgga ggccgggtca
     2401 gccaagtccc ccgccaggcc agcatcgtca tcgggcccta cagcggaaag aagaaagcca
     2461 cagtcaagta tctgtctgag aaatgggtct tagattccat cacccagcac aaggtctgtg
     2521 cccctgaaaa ctacctattg tcacaatgac agtgacctca ctggcctgtg gtgactgcac
     2581 acagctcgca aaactgtctt tggatgttca aatgagaaac aaaactgtga agagaaggaa
     2641 ctggcgtata caagatgact tctgatatca tgtttgccat gtgttgtggt tcttaagaac
     2701 tcataggtga ctttctgatg actgaatgtc tgtttcagag acgcttcggg cctttttatt
     2761 tttattttat tttttatttt ttgagacgga gtcctgccct gtttcccagg ctggagtgca
     2821 atggcacaat ctcggctcac tgcaacctcc acctcccagg ttcaagcgat tctgctgcct
     2881 cagcctcctg agtagctggg attacagatg tgtgccacca tgcctggcta atttttgtag
     2941 ttttagtaga gacagggttt cgccatgttg gccaggctgg tctcaaacgc ctgagctcag
     3001 gtgatctgtc aggcctcttc tatagaattc cagtctttgt gtcttagtca tgatcataat
     3061 tgaaaggtca cagaaccttt gtcattagag cacagtactg ccaaataaag aatggaaatt
     3121 caatgacatt gttttattac tgagaacaac tagagaactc tgcaagtttc ttggcttaga
     3181 ctcgatcttt attaatacat tatctattag gtaggaaaga catttgtcag ctattaaggt
     3241 gacttttatc tagcggagat tcctctctta aagtaatgaa aggagatagg tatggggggt
     3301 gttatacagg ataattggtg acatctgagt gtcttacttc tgcaagcctg ctttatggtg
     3361 agcaaagcat caccagcaag tgatcacaat gtccactggc cgctttttgc ctgccgtcct
     3421 cgagatgaaa ttggcagttg gggctgattc acagaaacac cgatttgtgg ctgagcacgg
     3481 tggctcacac ctgtcatccc agccctttgg gaggctgagg tggacagatc acttgaggtc
     3541 aggagttcga gaccagcctg accaacgcag caaaacccat ctctactaaa aatacaaaaa
     3601 tcagctgggt gtggtggcac acacctgtgg tcccagctcc tcaggagtct gaggcagaag
     3661 aatcgcttga acccaagagg cagaggttgc agtgagccaa ggttgcagtg aatcaagatt
     3721 gctccactgc actccagcct gggcaacaga gtaactctcc ttctcaaata aataaataaa
     3781 taaataagaa acactgatgt gtctgtcacc ttctaaagaa atgaaatgct aggaagtcct
     3841 agccagagtg atcaggcaag aataagccat aaaaggcatc caaataggaa aagaagtcaa
     3901 actgtctctc ttcactgccg atatgattct atacctagaa aaccctaaag actctgccaa
     3961 aaggctcctg gaaccgataa atgacttaag taaagtttca ggatagtaaa tccatgtaca
     4021 aaaatcagca tttccaaaca cagtaacatt caagctgagc accaaatcaa gaacgcaatc
     4081 ccatttccaa tagccacgga atgaaatacc taggaacacg tataaccaag gaggcaaagg
     4141 atctctacaa ggagaaccat aaacgagatg ctgagtccca gcgaggtcgg aggtgccact
     4201 gagccctcat cgtggtgccg ttcccgctct gggttattta tctgttgctc atctcagctg
     4261 ttgttcctac ctcaaatttc aagtccctca acaaatataa cagaaccact tctagaatga
     4321 acctttgaga agggaggtag cagtgcattg tataggaatt ggcattctat agaaaaccac
     4381 agaaactgga aataatgaag ggttgtctct tggttttaaa ataatgtata cacctaaatc
     4441 atccccttat gatactcatc ctctaacagc aattgaactt caatacaatg agtcattcct
     4501 gagttcactc gcttcacatt acatatgttt ctctataacc acaagcatcc tggcttggta
     4561 gtgctcccac agcaccaaaa atccctgagg aggctgacaa acattgtgct gactcatgct
     4621 ggagacaagc cacagagaac ttccatcccc caccacatca gccacggagc cagcccagcc
     4681 tctgcccacc caggcctcag tccccagtgt taagttctga tccctgatgc tggcctgcca
     4741 gtggccagtc aagattctct ttctgaaagc tagtatttta tgaggactga ctgttgctag
     4801 acattacact aagcacatta tatgttgtac ttcattttac cctttcaaca atcctattag
     4861 tagcttactg tgggtctgca aagccttact caaaacatat agggctagag gttctcagga
     4921 ttctgaattt taaaaaaaat ttgtaaaggc ttatggctct caccactgtt attcaacgtt
     4981 gcattaaagt ttctacccag agaaggcaat aaaaggaaat taaagctata cagattggaa
     5041 gtgaagaaat aaaagtcttt attctcaaga atacaagaca ctatgtatag aaattgtaag
     5101 gaatgcaaaa aaaaaaaaaa aaaaaagccc tacaagaact tataacaagt ttagcaagat
     5161 tgcaatatac aatcttgcaa tcttcctaaa gattatatac aaacctaaca gaattgtatt
     5221 tatatatact gtcaataagc aattcaaaat gaaattaaga ccacgattcc atttaaaatt
     5281 gcatctaaaa ataaacaaaa taggaataga cttggcaaca gttgtaacat ctgtatactg
     5341 aaacctgtaa aacattgctg aaagaagtta aagacttctt taaatagaga catatacaaa
     5401 gttcatagat tagaagatgc aatattgtta agatgatagt cctcaaattg acgtatagat
     5461 tcaatgcaat ccattaaaat ctcagatggc tttttataga atttgaaaag ctgatgctaa
     5521 atcttttatg aaaatgcaaa gaacctctag tagacaaaac aattttttta agagcaaagt
     5581 tggaggattt atagaacctg attccaaaac tgtcagtaaa actacaataa ttacaaagta
     5641 tcagccaggt gccgtggctc acatctgtaa taccagctct ctgggaggct gaggcgggtg
     5701 gatcacttga agtcgggagt ttaagaccag cctggccaac ttggtgaaac cttgtctcta
     5761 ctagaaatac aaaaaattag ccaggcatga tggcacacac ctgtaatctc agttactcag
     5821 gaggctgaag cagaatagct tgaacccaag agagtccagc tcaaaaacaa caacaagaac
     5881 aaaagtatca cattggcata agaatagaca tgtaaatcaa ataacaaaat agagaattca
     5941 ggtataaatc ttcatattta tggctgattg actttgaaca aaggtgacga ggcaagtcag
     6001 tatagcagca tattcttttc aacaaatggt gctggcagaa gaaaaaaaaa gtacttggat
     6061 ccttacctca caccatgcac aaaaattagc tcaacgtgga ctatatcagg gtttctcaac
     6121 atcagcagtg ttggcgtttg ctggggtgga taattctttc ctgtggagag ctgtcctgtg
     6181 cactgtagaa tgtttagctg catcctggcc tctgcctatt agatgccagt ggcatccctc
     6241 ccctgacaca cacctagttt tgacaatctc tgtctccaga tattcccaaa tgtgccctgg
     6301 agagcaaaac tgtttccagt tgagaaccat tgacctaatg taaaaaataa aatgtacaga
     6361 agaaagcata gcaggaaatc attctgacct caggttaggc aaagctttct tagatatgac
     6421 actaaaggca taattcatta aaaaaataag ttgggctttg tcaaaatttt aagttttgct
     6481 cttctgaaga aaccattaaa aaaaaatgaa aagacaagcc acagactatg aaaaaatact
     6541 tgcaaatcat atatctgata aaggacttgt acccagacca tgtaaagaac tcataactca
     6601 ttaataaaag gataaagaaa aagctgaaga caattcatgc aagaagatat acaaatatta
     6661 agcacattaa aatgctcaat attattagtc actagggaaa tacaaattaa aggcccaatg
     6721 aaatacctat tacacaccca ttagaatgac tgtaaacaac aagactgata attccaacat
     6781 ctgaccaaga tatggagaaa ctagaactct cctacattgc tggcatgagt gcaaaatgat
     6841 acagccactt tgaaacacca tttggcagtt gcttaaaaaa ttgaacattc aactaccata
     6901 tgacccagca atttcactct caggtatata accaaaatac atgaaaacat ggatctgcac
     6961 aaggtcttgt atacaaatgt tcatagcaac attattcata ataaccaaaa attagcccat
     7021 caactgaaaa gtagataaac aaaatgtata tccacaccat ggaacgctac tcagcaaact
     7081 tctaaaaagc aacaaactac taaacacgtg caacaacatg aatgagcctc agaaacataa
     7141 gtgaaagagg tcagacacaa aagactacaa attgtaagat tccatttaga taccatttct
     7201 acaaaccgca aaactgtaga ggcacgaaac agattcatgg tctgctgcag ctggggatgg
     7261 gagtgggaac agagagcaaa cattttggga tgatggaaac attctaaaac tggattgtga
     7321 ttttggttac acaactgtat acatttacta aaatcaatga acagaacact tttaatgggt
     7381 aagccttaag gcatgtgaat tatacaccaa taaagctatt ttttttaaaa aaaagagaga
     7441 gagagaacga gagctacatg gcagccccag ggcaatagct ctcagatctc ccttagagaa
     7501 agaactttct cttccacagc aatgagtgca ggtagctaac actgaccaac ctgcatctgg
     7561 tagttcattt aaggtcggcc ttggctttca agttaaggcc atggtctttc tctgcagtcc
     7621 ccagccaata cgtgggcaca gaaaggcaca gggcatggct gattcagccc aacatgaggc
     7681 tccacaccct agaactccct gtggagttgg ccgatactgc ctgtgacctg caatgtggtc
     7741 taagctgtca gacctccaca gtggccagaa ggttttccat acctgagcct gcttctaccc
     7801 acttttattt ttcccaggag tcacgcttag tcatctcttg ctctttgaac tccatgtctg
     7861 tatcagctac ccagaggacc catcagacag aaagtaatat gcagataaca gcccagggga
     7921 atctgggcag cactgtgaaa ccaacatttc ttcagtgaaa cctagaacaa aagtggaata
     7981 aataaaaact aactgtctca tttcagca
//
